MD08G1162100.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011016
Physical Location & Seq
Forward (+)
19021609 .. 19023083
1475 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1162100.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGCTTCCACCCTCTACCAATACTCAATCACTCAGCAAAACCAAGCCCTACCAAACACCCACCACCTCCAATCCTGCGACTGTTTCATCTTCCACTTCAACTTCACCCACCAACTCCAACTTTTCTTCCCCCAGGACTTGATTGTCCCACTCAATTCAATCCAACAATCGTTCCTTGTCCCCTTTCATGTGTTTTTCTCACAGACCCGAGAAACAATCGAGACCATTCTATCCGGGACTGGAGTTTCCATGGACTTTGTGGAGACTCTGGTGCCGGAAGTTTATGCTTTGGCCGTGGAGATTACCCGGAACTCTGAAAATGCTGAGCCGATAGTAGTCCCGCTGGATTTGGATGTCCTTGCTGTGACCCCTTTTGATGACCGGGAACAGATTGAGAGGGCTATTGTGGAATCTGGGCTGGTTTATAATCCTGTCCCGGCCGCCAAGTCATTGGGTGCCGGACTAAAGACTGTAAATATTGAGAATTTGGGTGTGAGCAAGGAATGTAGCATCTGTTTGGAGGAGCTTTCGACCGGATTGGAGGTTGTGCTCATGCCCTGCAATCATCTGTATCATAAAGATTGCATTGTTGAATGGCTGAAGAGAAGTCACATGTGCCCCTATTGCCGCTTCAAGATGCCCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

216

Amino Acids

24.31

Weight (kDa)

4.96

Isoelectric Point (pI)

57.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 165 - 210 3e-10 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 167 - 211 1.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 168 - 210 9.8e-15 Ring finger domain
zf-RING_11 PF17123 168 - 196 7.5e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 168 - 209 3.9e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 168 - 210 5.5e-06 zinc-RING finger domain
zf-C3HC4 PF00097 169 - 209 2.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 169 - 207 5e-08 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011016)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18760 AT1G18770 AT1G18780 AT1G21960 AT2G29840 AT3G13228
fragaria_vesca FvH4_2g27040
malus_domestica MD08G1162100.v1.1
prunus_persica Prupe.1G495200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0295911
rosa_laevigata RLG00000011757
rosa_multiflora Rmu_sc0003139.1_g000008
rosa_roxburghii Rroxscaffold_7G00172060
rosa_rugosa Rorug06G0255000 Rorug06G0255000
rosa_samantha Rh6AG368800 Rh6BG376500 Rh6CG382700 Rh6DG369300
rosa_wichuraiana Rw6G032160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 426
AasI GACNNNNNNGTC 1 cut(s) 143
AccB1I GGYRCC 2 cut(s) 271, 455
AciI CCGC 3 cut(s) 341, 441, 628
AcoI YGGCCR 2 cut(s) 291, 438
AcsI RAATTY 1 cut(s) 484
AcuI CTGAAG 1 cut(s) 620
AflIII ACRYGT 1 cut(s) 612
AgsI TTSAA 4 cut(s) 100, 159, 593, 634
AjnI CCWGG 1 cut(s) 132
AluBI AGCT 1 cut(s) 526
AluI AGCT 1 cut(s) 526
Alw21I GWGCWC 1 cut(s) 552
Alw26I GTCTC 2 cut(s) 215, 257
Ama87I CYCGRG 1 cut(s) 207
AoxI GGCC 2 cut(s) 291, 438
ApoI RAATTY 1 cut(s) 484
AsuC2I CCSGG 4 cut(s) 235, 307, 383, 437
AsuHPI GGTGA 1 cut(s) 97
AvaI CYCGRG 1 cut(s) 207
BaeGI GKGCMC 1 cut(s) 620
BanI GGYRCC 2 cut(s) 271, 455
Bbv12I GWGCWC 1 cut(s) 552
BceAI ACGGC 1 cut(s) 278
BciT130I CCWGG 1 cut(s) 134
BcnI CCSGG 4 cut(s) 235, 307, 383, 437
BcoDI GTCTC 2 cut(s) 215, 257
BisI GCNGC 2 cut(s) 441, 628
BlpI GCTNAGC 1 cut(s) 324
BlsI GCNGC 2 cut(s) 442, 629
Bme1390I CCNGG 5 cut(s) 134, 235, 307, 383, 437
BmeT110I CYCGRG 1 cut(s) 207
BmiI GGNNCC 2 cut(s) 273, 457
BmrFI CCNGG 5 cut(s) 134, 235, 307, 383, 437
BmsI GCATC 2 cut(s) 519, 627
BpmI CTGGAG 1 cut(s) 261
Bpu1102I GCTNAGC 1 cut(s) 324
BpuMI CCSGG 4 cut(s) 235, 307, 383, 437
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 3 cut(s) 132, 249, 294
BsaWI WCCGGW 1 cut(s) 533
Bse1I ACTGG 1 cut(s) 244
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 3 cut(s) 132, 249, 294
BseGI GGATG 1 cut(s) 358
BseMII CTCAG 2 cut(s) 47, 315
BseNI ACTGG 1 cut(s) 244
BseRI GAGGAG 1 cut(s) 536
BseSI GKGCMC 1 cut(s) 620
BseX3I CGGCCG 1 cut(s) 438
Bsh1285I CGRYCG 2 cut(s) 441, 534
BshFI GGCC 2 cut(s) 293, 440
BshNI GGYRCC 2 cut(s) 271, 455
BsiEI CGRYCG 2 cut(s) 441, 534
BsiHKAI GWGCWC 1 cut(s) 552
BsiHKCI CYCGRG 1 cut(s) 207
BsiSI CCGG 7 cut(s) 234, 275, 307, 382, 437, 459, 534
BslFI GGGAC 5 cut(s) 131, 164, 250, 323, 419
BsmAI GTCTC 2 cut(s) 215, 257
BsmFI GGGAC 5 cut(s) 131, 164, 250, 323, 419
BsnI GGCC 2 cut(s) 293, 440
Bso31I GGTCTC 1 cut(s) 215
BsoBI CYCGRG 1 cut(s) 207
Bsp1286I GDGCHC 2 cut(s) 552, 620
Bsp1720I GCTNAGC 1 cut(s) 324
Bsp19I CCATGG 1 cut(s) 249
BspACI CCGC 3 cut(s) 341, 441, 628
BspANI GGCC 2 cut(s) 293, 440
BspCNI CTCAG 2 cut(s) 46, 316
BspLI GGNNCC 2 cut(s) 273, 457
BspT107I GGYRCC 2 cut(s) 271, 455
BspTNI GGTCTC 1 cut(s) 215
BsrI ACTGG 1 cut(s) 244
BssECI CCNNGG 3 cut(s) 132, 249, 294
BssT1I CCWWGG 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 2 cut(s) 83, 472
Bst6I CTCTTC 1 cut(s) 596
BstDEI CTNAG 2 cut(s) 33, 324
BstDSI CCRYGG 2 cut(s) 249, 294
BstF5I GGATG 1 cut(s) 358
BstMAI GTCTC 2 cut(s) 215, 257
BstMCI CGRYCG 2 cut(s) 441, 534
BstMWI GCNNNNNNNGC 1 cut(s) 624
BstNI CCWGG 1 cut(s) 134
BstNSI RCATGY 1 cut(s) 616
BstSCI CCNGG 5 cut(s) 132, 233, 305, 381, 435
BstSLI GKGCMC 1 cut(s) 620
BstXI CCANNNNNNTGG 1 cut(s) 451
BstZI CGGCCG 1 cut(s) 438
BsuRI GGCC 2 cut(s) 293, 440
BtgI CCRYGG 2 cut(s) 249, 294
BtsCI GGATG 1 cut(s) 358
CviAII CATG 4 cut(s) 188, 250, 553, 613
CviJI RGCY 9 cut(s) 5, 47, 293, 328, 401, 418, 440, 526, 598
CviKI_1 RGCY 9 cut(s) 5, 47, 293, 328, 401, 418, 440, 526, 598
DdeI CTNAG 2 cut(s) 33, 324
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 2 cut(s) 291, 438
EagI CGGCCG 1 cut(s) 438
Eam1104I CTCTTC 1 cut(s) 596
EarI CTCTTC 1 cut(s) 596
EclXI CGGCCG 1 cut(s) 438
Eco130I CCWWGG 1 cut(s) 249
Eco31I GGTCTC 1 cut(s) 215
Eco52I CGGCCG 1 cut(s) 438
Eco57I CTGAAG 1 cut(s) 620
Eco88I CYCGRG 1 cut(s) 207
EcoRII CCWGG 1 cut(s) 132
EcoT14I CCWWGG 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 4 cut(s) 191, 253, 556, 616
FaiI YATR 7 cut(s) 189, 251, 285, 426, 554, 576, 614
FaqI GGGAC 5 cut(s) 131, 164, 250, 323, 419
FatI CATG 4 cut(s) 187, 249, 552, 612
FauI CCCGC 1 cut(s) 348
Fnu4HI GCNGC 2 cut(s) 441, 628
FokI GGATG 1 cut(s) 365
Fsp4HI GCNGC 2 cut(s) 441, 628
GluI GCNGC 2 cut(s) 441, 628
GsuI CTGGAG 1 cut(s) 261
HaeIII GGCC 2 cut(s) 293, 440
HapII CCGG 7 cut(s) 234, 275, 307, 382, 437, 459, 534
Hin1II CATG 4 cut(s) 191, 253, 556, 616
HinfI GANTC 2 cut(s) 265, 410
HpaII CCGG 7 cut(s) 234, 275, 307, 382, 437, 459, 534
HphI GGTGA 1 cut(s) 97
Hpy188I TCNGA 1 cut(s) 316
Hpy188III TCNNGA 2 cut(s) 220, 634
HpyCH4III ACNGT 2 cut(s) 83, 472
HpyCH4V TGCA 2 cut(s) 561, 585
HpyF10VI GCNNNNNNNGC 1 cut(s) 624
HpyF3I CTNAG 2 cut(s) 33, 324
Hsp92II CATG 4 cut(s) 191, 253, 556, 616
LmnI GCTCC 1 cut(s) 523
LweI GCATC 2 cut(s) 519, 627
MaeIII GTNAC 2 cut(s) 364, 608
MboII GAAGA 3 cut(s) 82, 118, 613
MhlI GDGCHC 2 cut(s) 552, 620
MluCI AATT 2 cut(s) 154, 484
MlyI GAGTC 1 cut(s) 259
MmeI TCCRAC 2 cut(s) 142, 187
MnlI CCTC 5 cut(s) 23, 77, 390, 514, 535
MspA1I CMGCKG 1 cut(s) 343
MspI CCGG 7 cut(s) 234, 275, 307, 382, 437, 459, 534
MspR9I CCNGG 5 cut(s) 134, 235, 307, 383, 437
MvaI CCWGG 1 cut(s) 134
MwoI GCNNNNNNNGC 1 cut(s) 624
NciI CCSGG 4 cut(s) 235, 307, 383, 437
NcoI CCATGG 1 cut(s) 249
NlaIII CATG 4 cut(s) 191, 253, 556, 616
NlaIV GGNNCC 2 cut(s) 273, 457
NmuCI GTSAC 2 cut(s) 364, 608
NspI RCATGY 1 cut(s) 616
PciI ACATGT 1 cut(s) 612
PfeI GAWTC 1 cut(s) 410
PfoI TCCNGGA 1 cut(s) 233
PkrI GCNGC 2 cut(s) 442, 629
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PscI ACATGT 1 cut(s) 612
PsiI TTATAA 1 cut(s) 426
Psp6I CCWGG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 2 cut(s) 273, 457
SatI GCNGC 2 cut(s) 441, 628
SchI GAGTC 1 cut(s) 259
ScrFI CCNGG 5 cut(s) 134, 235, 307, 383, 437
SduI GDGCHC 2 cut(s) 552, 620
SetI ASST 3 cut(s) 69, 528, 546
SfaNI GCATC 2 cut(s) 519, 627
Sse9I AATT 2 cut(s) 154, 484
SsiI CCGC 3 cut(s) 341, 441, 628
SspI AATATT 1 cut(s) 478
StyD4I CCNGG 5 cut(s) 132, 233, 305, 381, 435
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 2 cut(s) 83, 472
TaqI TCGA 2 cut(s) 219, 530
TasI AATT 2 cut(s) 154, 484
TauI GCSGC 2 cut(s) 443, 630
TfiI GAWTC 1 cut(s) 410
TseFI GTSAC 2 cut(s) 364, 608
Tsp45I GTSAC 2 cut(s) 364, 608
TspDTI ATGAA 2 cut(s) 76, 176
XapI RAATTY 1 cut(s) 484
XceI RCATGY 1 cut(s) 616
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.