AT1G22240

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012349
Physical Location & Seq
Forward (+)
7852904 .. 7855213
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G22240.1

Sequence Viewer

Length: 1548 bp
ATGATGAGAGGTGAATTTGGAGAAGCTTCGTCTCTTTCACGTTCTCCTTCTTCTCCTCTTCAAACAGAACCACATCCCCAATCTCCCAAGTTTTATCGTAATCATTATGTCTTTTCCGGTGATCACTCATCTTACAACAGTTCGAGTAGGTTCTGTCTCCGATCTCCGTCTGATTACTCGCTTTCGAGTTATTTCTCAAACGGGTTGTGTTCTTCTGAGGATGGTTCGTCTCAGTTCGCTTCTCCTCCACTCGATGGCTTGATGACCAAGTATAATTTTGGTGGTGATGATTTAGGTCTATGCGAAAGTTTCGATCTTCTGAATGTTGGAGAAGAAGACAAGACTCATCACCAAACTCAGAGGAGCAGTTTTGGTAACTATGAAGCTAATGGAGATGGTGGATTCCGATATTACTCTGGTTTTGGAGTTGATCAAAAGGATCATCAAATTTCTAATTCTTGGAATCTGGGAATGCAGAGTTGTAACATGAACAATTACTCTGTTCCGGTACATGGAAAAAGCGGTGTTGGAGCATTGTTTGATCATCAAGGAATTAGCAGTAACCCTAATGAGAGTCTGCCTAAAGTATCTGAATTCCAGGGATATGTTTATTTTATGGCTAAAGATCAACATGGTTGCAGATTCTTACAGTGGATCTTTGAAGATGGAAGTGCTTTAGATGCTCTGGTTATTTTCAGTGAAGTGATTCCTCATGTTGTTGAGCTTATGATGGATCCTTTTGGTAATTACTTGATGCAGAAACTATTAGATGTTTGTAATGAAGAACAAAGGACACAGATCATACTCATGGTCACTTCAGAACCTGGTCAGCTTATTCGAATATCTCTCAACGCTTATGGTACTCGAGTTGTTCAGAGATTAGTGGAATCAATCAAGACAAGGAAGCAGATTTCTCTGGTGAAATCAGCTTTGAGGCCTGGATTTCTTAATCTCATTAGGGATTTGAATGGGAATCATGTGATTCAACGTTGCTTGCAGTGCCTTAGTACAGAAGATAACGAGTTCATCTTTGAAGATGCTACAAAGTTCTGTATCGATATCGCTACACATAGACATGGATGTTGTGTGTTACAAAAGTGCATTGCTTATTCCAGTGGACTACAAAGAGAGAAGCTTGTAACCGAAATCTCAAGAAACAGTCTCTTCCTAGCTCAAGATCCTTATGGGAACTATGCTGTCCAATTTGTTCTTGAACTGAGGGATTTTTCTGCGATAGCCGCGATGCTTGCGCAGTTGAAAGGTCATTATGTAGAGCTTTCAATGCAGAAATTTAGTAGTCATATGGTGGAAAGATGCTTAACACATTGTCCAGAGAGCCGTCCTCAGATCGTGCGCGAACTCATCTCTGTTCCTCATTTTGATATTCTAATTCAAGACCCTTATGCTAACTTTGTCATCCAAGCTGCTCTTGCTGTTACCAAGGGTTCACTTCATGCCACACTCGTTGAAGTCATAAGGCCTCACTCGATTCTACGTAACAATCCTTATTGCAAAAGGATTTTCTCACGAAATCTCTTGAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

515

Amino Acids

58.01

Weight (kDa)

6.25

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 203 - 236 7.7e-07 Pumilio-family RNA binding repeat
PUF PF00806 238 - 269 5.3e-12 Pumilio-family RNA binding repeat
PUF PF00806 351 - 383 7.3e-13 Pumilio-family RNA binding repeat
PUF PF00806 422 - 455 2e-06 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012349)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22240 AT1G78160 AT1G78160
fragaria_vesca FvH4_5g37620
malus_domestica MD08G1181900.v1.1 MD15G1367200.v1.1
prunus_persica Prupe.1G515100_v2.0.a1
pyrus_communis pycom08g15510 pycom15g32920
rosa_chinensis RchiOBHm_Chr7g0239301
rosa_laevigata RLG00000000765
rosa_multiflora Rmu_sc0007840.1_g000004
rosa_roxburghii Rroxscaffold_3G00221040
rosa_rugosa Rorug07G0327100
rosa_samantha Rh7AG480500 Rh7BG453200 Rh7CG498400 Rh7DG466500
rosa_wichuraiana Rw7G040250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1251
AccB7I CCANNNNNTGG 1 cut(s) 254
AccII CGCG 2 cut(s) 1241, 1356
AciI CCGC 2 cut(s) 522, 1239
AclI AACGTT 1 cut(s) 988
AclWI GGATC 5 cut(s) 447, 662, 728, 741, 1172
AcsI RAATTY 4 cut(s) 14, 447, 593, 1289
AcuI CTGAAG 1 cut(s) 801
AfaI GTAC 3 cut(s) 510, 862, 1009
AfiI CCNNNNNNNGG 2 cut(s) 254, 512
AjnI CCWGG 3 cut(s) 597, 823, 937
AluBI AGCT 9 cut(s) 26, 386, 724, 832, 929, 1135, 1172, 1276, 1424
AluI AGCT 9 cut(s) 26, 386, 724, 832, 929, 1135, 1172, 1276, 1424
Alw26I GTCTC 4 cut(s) 36, 161, 234, 1166
AlwI GGATC 5 cut(s) 447, 662, 728, 741, 1172
AlwNI CAGNNNCTG 1 cut(s) 824
Ama87I CYCGRG 1 cut(s) 864
AoxI GGCC 2 cut(s) 935, 1478
ApeKI GCWGC 1 cut(s) 1424
ApoI RAATTY 4 cut(s) 14, 447, 593, 1289
Asp700I GAANNNNTTC 1 cut(s) 705
AspLEI GCGC 2 cut(s) 1252, 1356
AsuHPI GGTGA 5 cut(s) 23, 131, 296, 341, 931
AsuII TTCGAA 1 cut(s) 838
AvaI CYCGRG 1 cut(s) 864
BaeI ACNNNNGTAYC 2 cut(s) 852, 885
BamHI GGATCC 1 cut(s) 733
BbsI GAAGAC 1 cut(s) 342
BbvI GCAGC 1 cut(s) 1411
BccI CCATC 5 cut(s) 215, 248, 389, 659, 724
BceAI ACGGC 1 cut(s) 1323
BciT130I CCWGG 3 cut(s) 599, 825, 939
BclI TGATCA 3 cut(s) 121, 430, 541
BcoDI GTCTC 4 cut(s) 36, 161, 234, 1166
BfaI CTAG 1 cut(s) 1169
BisI GCNGC 2 cut(s) 1239, 1425
BlsI GCNGC 2 cut(s) 1240, 1426
Bme1390I CCNGG 3 cut(s) 599, 825, 939
BmeT110I CYCGRG 1 cut(s) 864
BmiI GGNNCC 1 cut(s) 735
BmrFI CCNGG 3 cut(s) 599, 825, 939
BmsI GCATC 5 cut(s) 670, 744, 1027, 1233, 1304
BpiI GAAGAC 1 cut(s) 342
BplI GAGNNNNNCTC 2 cut(s) 1327, 1359
Bpu14I TTCGAA 1 cut(s) 838
BpuEI CTTGAG 2 cut(s) 1135, 1158
Bsa29I ATCGAT 1 cut(s) 1056
BsaAI YACGTR 1 cut(s) 1496
BsaJI CCNNGG 2 cut(s) 598, 1440
BsaWI WCCGGW 2 cut(s) 116, 505
Bsc4I CCNNNNNNNGG 2 cut(s) 254, 512
Bse1I ACTGG 1 cut(s) 1113
Bse3DI GCAATG 1 cut(s) 1101
BseBI CCWGG 3 cut(s) 599, 825, 939
BseCI ATCGAT 1 cut(s) 1056
BseDI CCNNGG 2 cut(s) 598, 1440
BseGI GGATG 4 cut(s) 73, 226, 1085, 1416
BseLI CCNNNNNNNGG 2 cut(s) 254, 512
BseMI GCAATG 1 cut(s) 1101
BseMII CTCAG 5 cut(s) 207, 245, 371, 1208, 1358
BseNI ACTGG 1 cut(s) 1113
BseRI GAGGAG 3 cut(s) 45, 234, 376
BseXI GCAGC 1 cut(s) 1411
Bsh1236I CGCG 2 cut(s) 1241, 1356
BshFI GGCC 2 cut(s) 937, 1480
BshVI ATCGAT 1 cut(s) 1056
BsiHKCI CYCGRG 1 cut(s) 864
BsiSI CCGG 2 cut(s) 117, 506
BslI CCNNNNNNNGG 2 cut(s) 254, 512
BsmAI GTCTC 4 cut(s) 36, 161, 234, 1166
BsmBI CGTCTC 2 cut(s) 36, 234
BsmI GAATGC 1 cut(s) 477
BsnI GGCC 2 cut(s) 937, 1480
BsoBI CYCGRG 1 cut(s) 864
Bsp119I TTCGAA 1 cut(s) 838
BspACI CCGC 2 cut(s) 522, 1239
BspANI GGCC 2 cut(s) 937, 1480
BspCNI CTCAG 5 cut(s) 208, 244, 370, 1209, 1357
BspDI ATCGAT 1 cut(s) 1056
BspFNI CGCG 2 cut(s) 1241, 1356
BspLI GGNNCC 1 cut(s) 735
BspPI GGATC 5 cut(s) 447, 662, 728, 741, 1172
BspT104I TTCGAA 1 cut(s) 838
BsrDI GCAATG 1 cut(s) 1101
BsrI ACTGG 1 cut(s) 1113
BssECI CCNNGG 2 cut(s) 598, 1440
BssT1I CCWWGG 1 cut(s) 1440
Bst2UI CCWGG 3 cut(s) 599, 825, 939
Bst4CI ACNGT 3 cut(s) 140, 651, 1160
Bst6I CTCTTC 2 cut(s) 63, 1169
BstBAI YACGTR 1 cut(s) 1496
BstBI TTCGAA 1 cut(s) 838
BstC8I GCNNGC 2 cut(s) 995, 1248
BstDEI CTNAG 6 cut(s) 216, 231, 357, 1004, 1217, 1344
BstF5I GGATG 4 cut(s) 73, 226, 1085, 1416
BstFNI CGCG 2 cut(s) 1241, 1356
BstHHI GCGC 2 cut(s) 1252, 1356
BstMAI GTCTC 4 cut(s) 36, 161, 234, 1166
BstMWI GCNNNNNNNGC 6 cut(s) 680, 999, 1238, 1247, 1282, 1430
BstNI CCWGG 3 cut(s) 599, 825, 939
BstSCI CCNGG 3 cut(s) 597, 823, 937
BstSNI TACGTA 1 cut(s) 1496
BstUI CGCG 2 cut(s) 1241, 1356
BstV1I GCAGC 1 cut(s) 1411
BstV2I GAAGAC 1 cut(s) 342
BstX2I RGATCY 3 cut(s) 654, 733, 1177
BstYI RGATCY 3 cut(s) 654, 733, 1177
Bsu15I ATCGAT 1 cut(s) 1056
BsuRI GGCC 2 cut(s) 937, 1480
BsuTUI ATCGAT 1 cut(s) 1056
BtgZI GCGATG 1 cut(s) 1256
BtsCI GGATG 4 cut(s) 73, 226, 1085, 1416
BtsI GCAGTG 1 cut(s) 1004
BtsIMutI CAGTG 4 cut(s) 656, 703, 1004, 1120
Cac8I GCNNGC 2 cut(s) 995, 1248
CaiI CAGNNNCTG 1 cut(s) 824
CfoI GCGC 2 cut(s) 1252, 1356
ClaI ATCGAT 1 cut(s) 1056
CsiI ACCWGGT 1 cut(s) 823
Csp6I GTAC 3 cut(s) 509, 861, 1008
CspCI CAANNNNNGTGG 2 cut(s) 1447, 1482
CviAII CATG 8 cut(s) 487, 512, 632, 713, 808, 977, 1076, 1454
CviQI GTAC 3 cut(s) 509, 861, 1008
DdeI CTNAG 6 cut(s) 216, 231, 357, 1004, 1217, 1344
Eam1104I CTCTTC 2 cut(s) 63, 1169
EarI CTCTTC 2 cut(s) 63, 1169
Eco105I TACGTA 1 cut(s) 1496
Eco130I CCWWGG 1 cut(s) 1440
Eco147I AGGCCT 2 cut(s) 937, 1480
Eco32I GATATC 1 cut(s) 1060
Eco57I CTGAAG 1 cut(s) 801
Eco88I CYCGRG 1 cut(s) 864
EcoRI GAATTC 1 cut(s) 593
EcoRII CCWGG 3 cut(s) 597, 823, 937
EcoRV GATATC 1 cut(s) 1060
EcoT14I CCWWGG 1 cut(s) 1440
ErhI CCWWGG 1 cut(s) 1440
Esp3I CGTCTC 2 cut(s) 36, 234
FaeI CATG 8 cut(s) 490, 515, 635, 716, 811, 980, 1079, 1457
FalI AAGNNNNNCTT 2 cut(s) 1413, 1445
FatI CATG 8 cut(s) 486, 511, 631, 712, 807, 976, 1075, 1453
FauNDI CATATG 1 cut(s) 1302
FbaI TGATCA 3 cut(s) 121, 430, 541
Fnu4HI GCNGC 2 cut(s) 1239, 1425
FokI GGATG 4 cut(s) 60, 233, 1092, 1403
Fsp4HI GCNGC 2 cut(s) 1239, 1425
FspBI CTAG 1 cut(s) 1169
FspI TGCGCA 1 cut(s) 1251
GlaI GCGC 2 cut(s) 1251, 1355
GluI GCNGC 2 cut(s) 1239, 1425
HaeIII GGCC 2 cut(s) 937, 1480
HapII CCGG 2 cut(s) 117, 506
HhaI GCGC 2 cut(s) 1252, 1356
Hin1II CATG 8 cut(s) 490, 515, 635, 716, 811, 980, 1079, 1457
Hin6I GCGC 2 cut(s) 1250, 1354
HinP1I GCGC 2 cut(s) 1250, 1354
HindIII AAGCTT 2 cut(s) 24, 1133
HpaII CCGG 2 cut(s) 117, 506
HphI GGTGA 5 cut(s) 23, 131, 296, 341, 931
Hpy166II GTNNAC 2 cut(s) 1118, 1448
Hpy188III TCNNGA 8 cut(s) 895, 1152, 1175, 1211, 1331, 1394, 1527, 1537
Hpy8I GTNNAC 2 cut(s) 1118, 1448
HpyAV CCTTC 1 cut(s) 57
HpyCH4III ACNGT 3 cut(s) 140, 651, 1160
HpyCH4IV ACGT 3 cut(s) 40, 988, 1495
HpyCH4V TGCA 7 cut(s) 475, 639, 757, 997, 1101, 1285, 1512
HpyF10VI GCNNNNNNNGC 6 cut(s) 680, 999, 1238, 1247, 1282, 1430
HpyF3I CTNAG 6 cut(s) 216, 231, 357, 1004, 1217, 1344
HpySE526I ACGT 3 cut(s) 40, 988, 1495
Hsp92II CATG 8 cut(s) 490, 515, 635, 716, 811, 980, 1079, 1457
HspAI GCGC 2 cut(s) 1250, 1354
Ksp22I TGATCA 3 cut(s) 121, 430, 541
LmnI GCTCC 2 cut(s) 363, 530
Lsp1109I GCAGC 1 cut(s) 1411
LweI GCATC 5 cut(s) 670, 744, 1027, 1233, 1304
MabI ACCWGGT 1 cut(s) 823
MaeI CTAG 1 cut(s) 1169
MaeII ACGT 3 cut(s) 40, 988, 1495
MaeIII GTNAC 8 cut(s) 374, 482, 560, 811, 1089, 1138, 1435, 1496
MflI RGATCY 3 cut(s) 654, 733, 1177
MlyI GAGTC 2 cut(s) 337, 583
MmeI TCCRAC 2 cut(s) 307, 508
MroXI GAANNNNTTC 1 cut(s) 705
MseI TTAA 2 cut(s) 948, 1319
MslI CAYNNNNRTG 2 cut(s) 806, 1074
MspI CCGG 2 cut(s) 117, 506
MspR9I CCNGG 3 cut(s) 599, 825, 939
Mva1269I GAATGC 1 cut(s) 477
MvaI CCWGG 3 cut(s) 599, 825, 939
MvnI CGCG 2 cut(s) 1241, 1356
MwoI GCNNNNNNNGC 6 cut(s) 680, 999, 1238, 1247, 1282, 1430
NdeI CATATG 1 cut(s) 1302
NlaIII CATG 8 cut(s) 490, 515, 635, 716, 811, 980, 1079, 1457
NlaIV GGNNCC 1 cut(s) 735
NmuCI GTSAC 1 cut(s) 811
NsbI TGCGCA 1 cut(s) 1251
NspV TTCGAA 1 cut(s) 838
PaeR7I CTCGAG 1 cut(s) 864
PceI AGGCCT 2 cut(s) 937, 1480
PctI GAATGC 1 cut(s) 477
PdmI GAANNNNTTC 1 cut(s) 705
PfeI GAWTC 8 cut(s) 402, 463, 642, 706, 887, 973, 982, 1489
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 2 cut(s) 1240, 1426
PleI GAGTC 2 cut(s) 337, 582
PpsI GAGTC 2 cut(s) 337, 582
Ppu21I YACGTR 1 cut(s) 1496
Psp1406I AACGTT 1 cut(s) 988
Psp6I CCWGG 3 cut(s) 597, 823, 937
PspGI CCWGG 3 cut(s) 597, 823, 937
PspN4I GGNNCC 1 cut(s) 735
PspXI VCTCGAGB 1 cut(s) 864
PstNI CAGNNNCTG 1 cut(s) 824
PsuI RGATCY 3 cut(s) 654, 733, 1177
RsaI GTAC 3 cut(s) 510, 862, 1009
RsaNI GTAC 3 cut(s) 509, 861, 1008
RseI CAYNNNNRTG 2 cut(s) 806, 1074
SaqAI TTAA 2 cut(s) 948, 1319
SatI GCNGC 2 cut(s) 1239, 1425
SchI GAGTC 2 cut(s) 337, 583
ScrFI CCNGG 3 cut(s) 599, 825, 939
SexAI ACCWGGT 1 cut(s) 823
SfaNI GCATC 5 cut(s) 670, 744, 1027, 1233, 1304
Sfr274I CTCGAG 1 cut(s) 864
SfuI TTCGAA 1 cut(s) 838
SlaI CTCGAG 1 cut(s) 864
SmiMI CAYNNNNRTG 2 cut(s) 806, 1074
SmlI CTYRAG 3 cut(s) 864, 1150, 1173
SmoI CTYRAG 3 cut(s) 864, 1150, 1173
SnaBI TACGTA 1 cut(s) 1496
SseBI AGGCCT 2 cut(s) 937, 1480
SsiI CCGC 2 cut(s) 522, 1239
SspMI CTAG 1 cut(s) 1169
StuI AGGCCT 2 cut(s) 937, 1480
StyD4I CCNGG 3 cut(s) 597, 823, 937
StyI CCWWGG 1 cut(s) 1440
TaaI ACNGT 3 cut(s) 140, 651, 1160
TaiI ACGT 3 cut(s) 43, 991, 1498
TaqI TCGA 8 cut(s) 143, 185, 252, 312, 838, 865, 1056, 1487
TatI WGTACW 1 cut(s) 1007
TauI GCSGC 1 cut(s) 1241
TfiI GAWTC 8 cut(s) 402, 463, 642, 706, 887, 973, 982, 1489
Tru1I TTAA 2 cut(s) 948, 1319
Tru9I TTAA 2 cut(s) 948, 1319
TscAI CASTG 4 cut(s) 656, 703, 1004, 1120
TseFI GTSAC 1 cut(s) 811
TseI GCWGC 1 cut(s) 1424
Tsp45I GTSAC 1 cut(s) 811
TspDTI ATGAA 5 cut(s) 396, 503, 795, 1015, 1442
TspGWI ACGGA 1 cut(s) 156
TspRI CASTG 4 cut(s) 656, 703, 1004, 1120
Van91I CCANNNNNTGG 1 cut(s) 254
XapI RAATTY 4 cut(s) 14, 447, 593, 1289
XhoI CTCGAG 1 cut(s) 864
XmnI GAANNNNTTC 1 cut(s) 705
XspI CTAG 1 cut(s) 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.