MD08G1181900.v1.1

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012349
Physical Location & Seq
Forward (+)
22565213 .. 22569259
4047 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1181900.v1.1.491

Sequence Viewer

Length: 2154 bp
ATGTTCAAAATATCTGAGGAAGACAAGAGGGTTTTACAGAGGGAAGGAGGAGAGCTGGAAAAGCTCTGGAATGAGAATCCCACTAGGAATCAGCATTATTCCAGTCTTTTCCACCTCCTTCATCCTCCGCACCAACACCACCTTCTCGGCGGCTCTCCGGTGAGCAGAGCCTCTCTGCCGTCGGAGTTCTCTCCGTCGAGCTCTTTCTCAAATGGGTTCTATTCCTCCGGCTACGGTTCTCCGTCTCCGTATTCAACCCCATTTGAAGAGGCAAAGTATCAAACACCTTACGTGAACGGGTTGTGCCTCGACTCTAAGCCGCCCCCGGATGATCTGGGTTTGGCTGAGCGTTTCTATAGGATGCATATTGGGGAAGAACAGGAAAATGGGACTAAAAGGAGGGGATTTGAGAGAGGCCCAGATGGGATTGGACTCGGTGGTGGGTTTTTTTGGGGGACTAGTCCTTGGAATGCTGAAAACTATGGCCTATTTGAGAGTTCTAAGAACAATGTTTCGGATTTTGATGGGTTTCAATCTGCGGGTTTGGGAGGTAGTGGGAGATTTGGTAGGACTATGGAGTCAGCATTGCTTGGATTGCAGCCAAAATGCGCTGTTGGCGATTCAATGGGGTCCTTTCGCAATCATATGCAACCCAATGCTTTCTATTCTGGGCCTAGTTGTGTTAAAAGAATGGAACAGGGAGATGGCTGGTATCAGAGGAACCCATCTAGTGCTAGGTCTTACCCTGATGAGGACTATTTTTGCTTACAGCCACGTGGAACAGATTCTAGTGGCGGAAAGGGTCTTATGTATCCGATGAGTTCCCCTCAGTTCCCCTCGGTTGCTAAGTTGAATGTGGACAATCTTTCACATAACCATTCAATGATAAAACAAAGGACTGGAGTGAATCCAACTAGTGGGATTCCTGAATCTTTCAAGTCCATGAATTGTGCAGGGGACTTTGAGGGTTATGGTTGCGAGGATAGTTCCATCATGCAAGGAAACCATTTGAATCACGCCATATGTAAACCCAGCAAGTCTTCAAAGGGCTACAAGAAGAACTCTTGCACTAAGATGGCAATGCCGACAGGAGAGAAAAGCTCCAGACTTGATTCCGATTCATATGATGGTGGAAATGAATGGAGCCAGAGTGACAATAACTCATTTAGGTCATTTATAAGTTTGTGTTCATTGGCTGAGGTCCAGGAATATATATATTTCGTTGCAAAGGATCAGTACGGCTGTCGGTTCTTACAAGGGATGCTTGATGAGGGGACTAGCCAAGATTTGCAACTAATATTTGACAAAATAATCAACCATGTTTTCGAACTTATGACGAACCCATTTGGCAATTACCTTATGCAGAAGTTGTTGGATGTGTGCAATGAACAACAAAGAATGCAATTTGTAATCAAGGTGATAAAAGAACCAGGACAGCTTGTCAGAGTTTCCTTAGACACACATGGGACTCGTGTCGTACAGAAATTGATTGAGACTCTGAAACCTGGGAAACAGATAGCGTTAGTTAAATCGTCCCTTGAATGCGGTTTTCTTGATCTTATTAAGGATCCAAATGGCAATCATGTAGTACAACGTTGCTTGGATTGCTTTAGCGACGAAGACAATAAGTTTATTTTTGATGCTGCTGCAAGATATTGTGTCGAGATTGCAACTCAACGCCATGGATGCTGTGTGCTACAAAAATGCATAGCACATGCCATTGGGAGACATCGAGATAAGCTGATCAATGAAATTTCAAGAAATGGGCTTCTCCTTTCACAAGATCCTTATGGGAATTATGTCGTTCAATATGTGATAGAGCTGAAGATCCCATCTGCCATGGCCAAACTGACTACTCAGTTAAAAGGAAATTTTGTACGCCTCTCCATGCAGAAGTGCAGTAGTCATGTTGTTGAAAAGTGCCTCAAGTATTTTGAAGAGAGCCGACCTAAAATCATCCACGAATTGCTCTCATTTCAGCGTTTTGATCAGTTGCTACAAGACCCTTACGCAAACTACGTGATTCAATCTGCTCTTGGAGTTGCAAAGGGCCCTCTTCATGCTGCTCTGGTTGAAGCAGTGAAGCCTCATACAATCTTACGCACCAGTCCATACTGCAAGAGGATTTTCTCAAAGGACCTTCTGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

718

Amino Acids

80.29

Weight (kDa)

7.95

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 405 - 438 6.2e-09 Pumilio-family RNA binding repeat
PUF PF00806 440 - 470 4.3e-09 Pumilio-family RNA binding repeat
PUF PF00806 517 - 547 1.5e-08 Pumilio-family RNA binding repeat
PUF PF00806 552 - 585 1.9e-12 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012349)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22240 AT1G78160 AT1G78160
fragaria_vesca FvH4_5g37620
malus_domestica MD08G1181900.v1.1 MD15G1367200.v1.1
prunus_persica Prupe.1G515100_v2.0.a1
pyrus_communis pycom08g15510 pycom15g32920
rosa_chinensis RchiOBHm_Chr7g0239301
rosa_laevigata RLG00000000765
rosa_multiflora Rmu_sc0007840.1_g000004
rosa_roxburghii Rroxscaffold_3G00221040
rosa_rugosa Rorug07G0327100
rosa_samantha Rh7AG480500 Rh7BG453200 Rh7CG498400 Rh7DG466500
rosa_wichuraiana Rw7G040250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1178
AasI GACNNNNNNGTC 1 cut(s) 577
AccB7I CCANNNNNTGG 1 cut(s) 917
AciI CCGC 6 cut(s) 128, 150, 320, 539, 795, 1545
AclI AACGTT 1 cut(s) 1594
AclWI GGATC 5 cut(s) 1239, 1562, 1575, 1778, 1822
AcoI YGGCCR 1 cut(s) 1842
AcsI RAATTY 2 cut(s) 1752, 1870
AcuI CTGAAG 1 cut(s) 1844
AcvI CACGTG 1 cut(s) 776
AfaI GTAC 4 cut(s) 1238, 1479, 1590, 1878
AfiI CCNNNNNNNGG 2 cut(s) 751, 917
AhdI GACNNNNNGTC 1 cut(s) 1439
AhlI ACTAGT 2 cut(s) 458, 914
AjnI CCWGG 3 cut(s) 1203, 1429, 1504
AjuI GAANNNNNNNTTGG 2 cut(s) 126, 158
AluBI AGCT 7 cut(s) 55, 64, 201, 1101, 1438, 1741, 1822
AluI AGCT 7 cut(s) 55, 64, 201, 1101, 1438, 1741, 1822
Alw21I GWGCWC 1 cut(s) 203
Alw26I GTCTC 3 cut(s) 249, 1487, 1720
AlwI GGATC 5 cut(s) 1239, 1562, 1575, 1778, 1822
AoxI GGCC 5 cut(s) 415, 484, 671, 1842, 2050
ApaI GGGCCC 1 cut(s) 2054
ApeKI GCWGC 4 cut(s) 598, 1643, 1646, 2063
ApoI RAATTY 2 cut(s) 1752, 1870
Asp700I GAANNNNTTC 1 cut(s) 784
AspLEI GCGC 1 cut(s) 611
AspS9I GGNCC 7 cut(s) 416, 630, 671, 1201, 2050, 2051, 2137
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 2 cut(s) 172, 1429
AsuII TTCGAA 1 cut(s) 1326
AvaII GGWCC 3 cut(s) 630, 1201, 2137
BaeGI GKGCMC 1 cut(s) 2054
BalI TGGCCA 1 cut(s) 1844
BamHI GGATCC 1 cut(s) 1567
BanII GRGCYC 2 cut(s) 203, 2054
BauI CACGAG 1 cut(s) 1470
BbrPI CACGTG 1 cut(s) 776
BbsI GAAGAC 3 cut(s) 27, 1032, 1626
Bbv12I GWGCWC 1 cut(s) 203
BbvCI CCTCAGC 1 cut(s) 1197
BbvI GCAGC 4 cut(s) 610, 1630, 1633, 2050
BccI CCATC 8 cut(s) 416, 518, 698, 733, 998, 1069, 1121, 1840
BceAI ACGGC 2 cut(s) 163, 1255
BciT130I CCWGG 3 cut(s) 1205, 1431, 1506
BciVI GTATCC 1 cut(s) 822
BclI TGATCA 2 cut(s) 1743, 1987
BcnI CCSGG 1 cut(s) 326
BcoDI GTCTC 3 cut(s) 249, 1487, 1720
BcuI ACTAGT 2 cut(s) 458, 914
BfaI CTAG 8 cut(s) 84, 459, 675, 729, 735, 789, 915, 1278
BfmI CTRYAG 1 cut(s) 355
BfuI GTATCC 1 cut(s) 822
BisI GCNGC 6 cut(s) 151, 320, 599, 1644, 1647, 2064
BlpI GCTNAGC 1 cut(s) 345
BlsI GCNGC 6 cut(s) 152, 321, 600, 1645, 1648, 2065
Bme1390I CCNGG 4 cut(s) 326, 1205, 1431, 1506
Bme18I GGWCC 3 cut(s) 630, 1201, 2137
BmeRI GACNNNNNGTC 1 cut(s) 1439
BmgT120I GGNCC 7 cut(s) 416, 630, 671, 1201, 2050, 2051, 2137
BmiI GGNNCC 5 cut(s) 631, 722, 1145, 1569, 2052
BmrFI CCNGG 4 cut(s) 326, 1205, 1431, 1506
BmsI GCATC 4 cut(s) 351, 1251, 1630, 1676
BoxI GACNNNNGTC 1 cut(s) 1472
BpiI GAAGAC 3 cut(s) 27, 1032, 1626
BplI GAGNNNNNCTC 4 cut(s) 811, 843, 1085, 1117
BpmI CTGGAG 2 cut(s) 921, 1087
Bpu10I CCTNAGC 1 cut(s) 1197
Bpu1102I GCTNAGC 1 cut(s) 345
Bpu14I TTCGAA 1 cut(s) 1326
BpuEI CTTGAG 1 cut(s) 1910
BpuMI CCSGG 1 cut(s) 326
BsaAI YACGTR 3 cut(s) 292, 776, 2020
BsaJI CCNNGG 6 cut(s) 324, 464, 837, 1505, 1681, 1839
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 4 cut(s) 550, 580, 1135, 1165
Bsc4I CCNNNNNNNGG 2 cut(s) 751, 917
Bse1I ACTGG 3 cut(s) 102, 904, 2106
Bse3DI GCAATG 3 cut(s) 584, 1086, 1390
BseBI CCWGG 3 cut(s) 1205, 1431, 1506
BseDI CCNNGG 6 cut(s) 324, 464, 837, 1505, 1681, 1839
BseGI GGATG 7 cut(s) 121, 334, 366, 1266, 1381, 1691, 1956
BseLI CCNNNNNNNGG 2 cut(s) 751, 917
BseMI GCAATG 3 cut(s) 584, 1086, 1390
BseMII CTCAG 5 cut(s) 6, 336, 842, 1188, 1871
BseNI ACTGG 3 cut(s) 102, 904, 2106
BseRI GAGGAG 1 cut(s) 63
BseSI GKGCMC 1 cut(s) 2054
BseXI GCAGC 4 cut(s) 610, 1630, 1633, 2050
BseYI CCCAGC 1 cut(s) 1031
BsgI GTGCAG 2 cut(s) 972, 1918
BshFI GGCC 5 cut(s) 417, 486, 673, 1844, 2052
BsiHKAI GWGCWC 1 cut(s) 203
BsiSI CCGG 3 cut(s) 158, 228, 326
BslFI GGGAC 6 cut(s) 403, 469, 971, 1288, 1480, 1519
BslI CCNNNNNNNGG 2 cut(s) 751, 917
BsmAI GTCTC 3 cut(s) 249, 1487, 1720
BsmBI CGTCTC 1 cut(s) 249
BsmFI GGGAC 6 cut(s) 403, 469, 971, 1288, 1480, 1519
BsmI GAATGC 3 cut(s) 475, 1404, 1547
BsnI GGCC 5 cut(s) 417, 486, 673, 1844, 2052
Bsp119I TTCGAA 1 cut(s) 1326
Bsp120I GGGCCC 1 cut(s) 2050
Bsp1286I GDGCHC 2 cut(s) 203, 2054
Bsp143I GATC 8 cut(s) 331, 1231, 1555, 1567, 1743, 1783, 1827, 1987
Bsp1720I GCTNAGC 1 cut(s) 345
Bsp19I CCATGG 2 cut(s) 1681, 1839
BspACI CCGC 6 cut(s) 128, 150, 320, 539, 795, 1545
BspANI GGCC 5 cut(s) 417, 486, 673, 1844, 2052
BspCNI CTCAG 5 cut(s) 7, 337, 841, 1189, 1870
BspLI GGNNCC 5 cut(s) 631, 722, 1145, 1569, 2052
BspPI GGATC 5 cut(s) 1239, 1562, 1575, 1778, 1822
BspT104I TTCGAA 1 cut(s) 1326
BsrDI GCAATG 3 cut(s) 584, 1086, 1390
BsrI ACTGG 3 cut(s) 102, 904, 2106
BssECI CCNNGG 6 cut(s) 324, 464, 837, 1505, 1681, 1839
BssMI GATC 8 cut(s) 331, 1231, 1555, 1567, 1743, 1783, 1827, 1987
BssSI CACGAG 1 cut(s) 1470
BssT1I CCWWGG 3 cut(s) 464, 1681, 1839
Bst2BI CACGAG 1 cut(s) 1470
Bst2UI CCWGG 3 cut(s) 1205, 1431, 1506
Bst4CI ACNGT 1 cut(s) 236
Bst6I CTCTTC 3 cut(s) 261, 1932, 2061
BstBAI YACGTR 3 cut(s) 292, 776, 2020
BstBI TTCGAA 1 cut(s) 1326
BstDSI CCRYGG 2 cut(s) 1681, 1839
BstF5I GGATG 7 cut(s) 121, 334, 366, 1266, 1381, 1691, 1956
BstHHI GCGC 1 cut(s) 611
BstKTI GATC 8 cut(s) 334, 1234, 1558, 1570, 1746, 1786, 1830, 1990
BstMAI GTCTC 3 cut(s) 249, 1487, 1720
BstMBI GATC 8 cut(s) 331, 1231, 1555, 1567, 1743, 1783, 1827, 1987
BstMWI GCNNNNNNNGC 5 cut(s) 61, 595, 615, 1605, 1686
BstNI CCWGG 3 cut(s) 1205, 1431, 1506
BstNSI RCATGY 1 cut(s) 1718
BstPAI GACNNNNGTC 1 cut(s) 1472
BstSCI CCNGG 4 cut(s) 324, 1203, 1429, 1504
BstSFI CTRYAG 1 cut(s) 355
BstSLI GKGCMC 1 cut(s) 2054
BstV1I GCAGC 4 cut(s) 610, 1630, 1633, 2050
BstV2I GAAGAC 3 cut(s) 27, 1032, 1626
BstX2I RGATCY 3 cut(s) 1567, 1783, 1827
BstYI RGATCY 3 cut(s) 1567, 1783, 1827
BsuI GTATCC 1 cut(s) 822
BsuRI GGCC 5 cut(s) 417, 486, 673, 1844, 2052
BtgI CCRYGG 2 cut(s) 1681, 1839
BtsCI GGATG 7 cut(s) 121, 334, 366, 1266, 1381, 1691, 1956
BtsI GCAGTG 1 cut(s) 2085
BtsIMutI CAGTG 1 cut(s) 2085
CfoI GCGC 1 cut(s) 611
Cfr13I GGNCC 7 cut(s) 416, 630, 671, 1201, 2050, 2051, 2137
Csp6I GTAC 4 cut(s) 1237, 1478, 1589, 1877
CviQI GTAC 4 cut(s) 1237, 1478, 1589, 1877
DpnI GATC 8 cut(s) 333, 1233, 1557, 1569, 1745, 1785, 1829, 1989
DpnII GATC 8 cut(s) 331, 1231, 1555, 1567, 1743, 1783, 1827, 1987
DrdI GACNNNNNNGTC 1 cut(s) 577
DriI GACNNNNNGTC 1 cut(s) 1439
DseDI GACNNNNNNGTC 1 cut(s) 577
EaeI YGGCCR 1 cut(s) 1842
Eam1104I CTCTTC 3 cut(s) 261, 1932, 2061
Eam1105I GACNNNNNGTC 1 cut(s) 1439
EarI CTCTTC 3 cut(s) 261, 1932, 2061
EciI GGCGGA 1 cut(s) 810
Ecl136II GAGCTC 1 cut(s) 201
Eco130I CCWWGG 3 cut(s) 464, 1681, 1839
Eco24I GRGCYC 2 cut(s) 203, 2054
Eco47I GGWCC 3 cut(s) 630, 1201, 2137
Eco53kI GAGCTC 1 cut(s) 201
Eco57I CTGAAG 1 cut(s) 1844
Eco72I CACGTG 1 cut(s) 776
EcoICRI GAGCTC 1 cut(s) 201
EcoO109I RGGNCCY 4 cut(s) 630, 2050, 2051, 2137
EcoRII CCWGG 3 cut(s) 1203, 1429, 1504
EcoT14I CCWWGG 3 cut(s) 464, 1681, 1839
EcoT22I ATGCAT 2 cut(s) 366, 1709
EcoT38I GRGCYC 2 cut(s) 203, 2054
ErhI CCWWGG 3 cut(s) 464, 1681, 1839
Esp3I CGTCTC 1 cut(s) 249
FalI AAGNNNNNCTT 2 cut(s) 1248, 1280
FaqI GGGAC 6 cut(s) 403, 469, 971, 1288, 1480, 1519
FauI CCCGC 1 cut(s) 532
FauNDI CATATG 3 cut(s) 645, 1022, 1123
FbaI TGATCA 2 cut(s) 1743, 1987
Fnu4HI GCNGC 6 cut(s) 151, 320, 599, 1644, 1647, 2064
FokI GGATG 7 cut(s) 108, 341, 373, 1273, 1388, 1698, 1943
FriOI GRGCYC 2 cut(s) 203, 2054
Fsp4HI GCNGC 6 cut(s) 151, 320, 599, 1644, 1647, 2064
FspBI CTAG 8 cut(s) 84, 459, 675, 729, 735, 789, 915, 1278
GlaI GCGC 1 cut(s) 610
GluI GCNGC 6 cut(s) 151, 320, 599, 1644, 1647, 2064
GsaI CCCAGC 1 cut(s) 1035
GsuI CTGGAG 2 cut(s) 921, 1087
HaeIII GGCC 5 cut(s) 417, 486, 673, 1844, 2052
HapII CCGG 3 cut(s) 158, 228, 326
HhaI GCGC 1 cut(s) 611
Hin6I GCGC 1 cut(s) 609
HinP1I GCGC 1 cut(s) 609
HpaII CCGG 3 cut(s) 158, 228, 326
HphI GGTGA 2 cut(s) 172, 1429
Hpy166II GTNNAC 3 cut(s) 295, 859, 1028
Hpy188I TCNGA 9 cut(s) 16, 184, 517, 717, 816, 1117, 1445, 1500, 2145
Hpy188III TCNNGA 7 cut(s) 67, 926, 1104, 1553, 1663, 1733, 1758
Hpy8I GTNNAC 3 cut(s) 295, 859, 1028
Hpy99I CGWCG 3 cut(s) 184, 199, 1619
HpyAV CCTTC 4 cut(s) 38, 128, 152, 2150
HpyCH4III ACNGT 1 cut(s) 236
HpyCH4IV ACGT 4 cut(s) 291, 775, 1594, 2019
HpyF10VI GCNNNNNNNGC 5 cut(s) 61, 595, 615, 1605, 1686
HpySE526I ACGT 4 cut(s) 291, 775, 1594, 2019
HspAI GCGC 1 cut(s) 609
Ksp22I TGATCA 2 cut(s) 1743, 1987
Kzo9I GATC 8 cut(s) 331, 1231, 1555, 1567, 1743, 1783, 1827, 1987
LmnI GCTCC 2 cut(s) 1106, 1143
Lsp1109I GCAGC 4 cut(s) 610, 1630, 1633, 2050
LweI GCATC 4 cut(s) 351, 1251, 1630, 1676
MaeI CTAG 8 cut(s) 84, 459, 675, 729, 735, 789, 915, 1278
MaeII ACGT 4 cut(s) 291, 775, 1594, 2019
MaeIII GTNAC 1 cut(s) 1151
MalI GATC 8 cut(s) 333, 1233, 1557, 1569, 1745, 1785, 1829, 1989
MboI GATC 8 cut(s) 331, 1231, 1555, 1567, 1743, 1783, 1827, 1987
MboII GAAGA 9 cut(s) 32, 278, 386, 1032, 1069, 1631, 1837, 1949, 2048
MflI RGATCY 3 cut(s) 1567, 1783, 1827
MhlI GDGCHC 2 cut(s) 203, 2054
MlsI TGGCCA 1 cut(s) 1844
MluCI AATT 8 cut(s) 946, 1351, 1403, 1484, 1752, 1795, 1870, 1964
MluNI TGGCCA 1 cut(s) 1844
MlyI GAGTC 5 cut(s) 305, 426, 587, 1462, 1489
MmeI TCCRAC 3 cut(s) 162, 935, 1353
Mox20I TGGCCA 1 cut(s) 1844
Mph1103I ATGCAT 2 cut(s) 366, 1709
MroXI GAANNNNTTC 1 cut(s) 784
MscI TGGCCA 1 cut(s) 1844
MseI TTAA 4 cut(s) 684, 1527, 1563, 1862
MslI CAYNNNNRTG 1 cut(s) 1073
Msp20I TGGCCA 1 cut(s) 1844
MspI CCGG 3 cut(s) 158, 228, 326
MspR9I CCNGG 4 cut(s) 326, 1205, 1431, 1506
Mva1269I GAATGC 3 cut(s) 475, 1404, 1547
MvaI CCWGG 3 cut(s) 1205, 1431, 1506
MwoI GCNNNNNNNGC 5 cut(s) 61, 595, 615, 1605, 1686
NciI CCSGG 1 cut(s) 326
NcoI CCATGG 2 cut(s) 1681, 1839
NdeI CATATG 3 cut(s) 645, 1022, 1123
NdeII GATC 8 cut(s) 331, 1231, 1555, 1567, 1743, 1783, 1827, 1987
NlaIV GGNNCC 5 cut(s) 631, 722, 1145, 1569, 2052
NmeAIII GCCGAG 1 cut(s) 126
NmuCI GTSAC 1 cut(s) 1151
NsiI ATGCAT 2 cut(s) 366, 1709
NspI RCATGY 1 cut(s) 1718
NspV TTCGAA 1 cut(s) 1326
PcsI WCGNNNNNNNCGW 1 cut(s) 2016
PctI GAATGC 3 cut(s) 475, 1404, 1547
PdmI GAANNNNTTC 1 cut(s) 784
PflMI CCANNNNNTGG 1 cut(s) 917
PfoI TCCNGGA 1 cut(s) 1203
PkrI GCNGC 6 cut(s) 152, 321, 600, 1645, 1648, 2065
PleI GAGTC 5 cut(s) 305, 426, 586, 1462, 1489
PmaCI CACGTG 1 cut(s) 776
PmlI CACGTG 1 cut(s) 776
PpsI GAGTC 5 cut(s) 305, 426, 586, 1462, 1489
Ppu21I YACGTR 3 cut(s) 292, 776, 2020
PpuMI RGGWCCY 2 cut(s) 630, 2137
PshAI GACNNNNGTC 1 cut(s) 1472
PsiI TTATAA 1 cut(s) 1178
Psp124BI GAGCTC 1 cut(s) 203
Psp1406I AACGTT 1 cut(s) 1594
Psp5II RGGWCCY 2 cut(s) 630, 2137
Psp6I CCWGG 3 cut(s) 1203, 1429, 1504
PspCI CACGTG 1 cut(s) 776
PspFI CCCAGC 1 cut(s) 1031
PspGI CCWGG 3 cut(s) 1203, 1429, 1504
PspN4I GGNNCC 5 cut(s) 631, 722, 1145, 1569, 2052
PspOMI GGGCCC 1 cut(s) 2050
PspPI GGNCC 7 cut(s) 416, 630, 671, 1201, 2050, 2051, 2137
PspPPI RGGWCCY 2 cut(s) 630, 2137
PsuI RGATCY 3 cut(s) 1567, 1783, 1827
RsaI GTAC 4 cut(s) 1238, 1479, 1590, 1878
RsaNI GTAC 4 cut(s) 1237, 1478, 1589, 1877
RseI CAYNNNNRTG 1 cut(s) 1073
SacI GAGCTC 1 cut(s) 203
SaqAI TTAA 4 cut(s) 684, 1527, 1563, 1862
SatI GCNGC 6 cut(s) 151, 320, 599, 1644, 1647, 2064
Sau3AI GATC 8 cut(s) 331, 1231, 1555, 1567, 1743, 1783, 1827, 1987
Sau96I GGNCC 7 cut(s) 416, 630, 671, 1201, 2050, 2051, 2137
SchI GAGTC 5 cut(s) 305, 426, 587, 1462, 1489
ScrFI CCNGG 4 cut(s) 326, 1205, 1431, 1506
SduI GDGCHC 2 cut(s) 203, 2054
SfaNI GCATC 4 cut(s) 351, 1251, 1630, 1676
SfcI CTRYAG 1 cut(s) 355
SfuI TTCGAA 1 cut(s) 1326
SinI GGWCC 3 cut(s) 630, 1201, 2137
SmiMI CAYNNNNRTG 1 cut(s) 1073
SmlI CTYRAG 1 cut(s) 1925
SmoI CTYRAG 1 cut(s) 1925
SpeI ACTAGT 2 cut(s) 458, 914
Sse9I AATT 8 cut(s) 946, 1351, 1403, 1484, 1752, 1795, 1870, 1964
SsiI CCGC 6 cut(s) 128, 150, 320, 539, 795, 1545
SspI AATATT 1 cut(s) 1299
SspMI CTAG 8 cut(s) 84, 459, 675, 729, 735, 789, 915, 1278
SstI GAGCTC 1 cut(s) 203
StyD4I CCNGG 4 cut(s) 324, 1203, 1429, 1504
StyI CCWWGG 3 cut(s) 464, 1681, 1839
TaaI ACNGT 1 cut(s) 236
TaiI ACGT 4 cut(s) 294, 778, 1597, 2022
TaqI TCGA 5 cut(s) 197, 309, 1326, 1662, 1732
TasI AATT 8 cut(s) 946, 1351, 1403, 1484, 1752, 1795, 1870, 1964
TatI WGTACW 1 cut(s) 1588
TauI GCSGC 2 cut(s) 153, 322
Tru1I TTAA 4 cut(s) 684, 1527, 1563, 1862
Tru9I TTAA 4 cut(s) 684, 1527, 1563, 1862
TscAI CASTG 1 cut(s) 2085
TseFI GTSAC 1 cut(s) 1151
TseI GCWGC 4 cut(s) 598, 1643, 1646, 2063
Tsp45I GTSAC 1 cut(s) 1151
TspDTI ATGAA 8 cut(s) 110, 959, 1110, 1152, 1179, 1401, 1764, 2048
TspGWI ACGGA 3 cut(s) 183, 231, 237
TspRI CASTG 1 cut(s) 2085
Van91I CCANNNNNTGG 1 cut(s) 917
VpaK11BI GGWCC 3 cut(s) 630, 1201, 2137
XapI RAATTY 2 cut(s) 1752, 1870
XceI RCATGY 1 cut(s) 1718
XmnI GAANNNNTTC 1 cut(s) 784
XspI CTAG 8 cut(s) 84, 459, 675, 729, 735, 789, 915, 1278
Zsp2I ATGCAT 2 cut(s) 366, 1709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.