FvH4_5g37620

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012349
Physical Location & Seq
Reverse (-)
27688511 .. 27692960
4450 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g37620.t1

Sequence Viewer

Length: 2250 bp
ATGTTAAAATTAGCTGAGCAAGACCAGAGGGTCTTGATGAACATGAGGAATGATAACAACAACAATCTTAGAAACCCTGCTTCACCTTATTGTAATTCCAATCTTTTTCACCTTCTGCTTCAGCCTAACCAGCATCACAACCATGTGAGTGGTTCTTCACCTATTAGCAGAGCTTCTCTGCAATCTGAGTTTTCTCCTTCAAGCTCTTTCTCAAATGGGTGTTATTCCTCTGGGGATAGCTCTCCTTATTCTCCTTCAACCCCATTTGTGGAGGCAAAGTATCAGACACCTTTTCAGCATTACATGAATGGAGGAGGAGGGTTGTGGTTGGACTCCAAGCCAGCACCAGCATCACATGAGTCTCATTTCAATGAGATTTTGGGGGATGATCTGGGGTTGGCTGAGAGTTTTTACAGACTGAGTATTGGGGATGAACATGAGGGTGGTGTTAGAAGGAGGGGGTTTGAGAGAGACCCAGGTGGGTTTGGGCTTGGTGGTGGTGGTGGTTTCTTAGGGGGTAATGTTCCTTGGAATGTTGAGGACTATGGCTTGTTTGAAATTTCTAGGAACAATATGTCTGATGTTGAAGGTTATCAGTCCTCTCATTTTGGTGTTAGGGGTGGTTTTAATGGTTATGTGGAGCCTGAGTTTCTTGGGTTGGGGCCAGAATATGCTCTTGGTGATTCAATGGGGACTGTTCTTAATCATATGCAGCAGCAGCCTAGTGCTATGTGTTGTGGTCCCAGTGGTGTGAAGAACCAAATGGGTTACATGTTGGAGCAAAGAAAGGAACTGAGAGATGAATGGTATTACAGAAATGTTCAAGTAGAGAACCCATATTTTGCCAGGCCTTACCTTGATGATGAATTTGTTCATTCACAGTTATGTGGAACAGATTCTAATGGCGGAAAGGGTCATATGTATCCGATGAGCTCCCCTCAGTTCCCCTCAGTTGCTCAGTTGTGTGTGGACAATGGTTCACATAATCATCCAGTGATAAAACAAAGGACTAGAGGAACCCCAACTAGGAAATGTGCAGGGCACCTAGAGGGTCTTAGAGGTGAGGATAATCTTGTTATACAAGGTAAACATTTGAATCATGGTGTCACTAAGGGTTGCAAGTCTTCAAAGGCTAACAAGAAGAACTCTTGCACTAAGATGGCAATGAGGACAGAAGGACAGAAAAGCGCTAGACTAGATTTGGATTCATATAATGGAGGAAGCAGTGGAAATGAATGTAGTCAGAGCAGTAATGACTCAGTGAAGTCACTGCTTTCTTTGGGTTCATTGACTGACGTCCAGGGATACATATACTTCCTATCAAAGGATCAGCACGGTTGCCGCTTCTTACAGAGAATGCTCGATGAGGGAACCCGTCAAGATGTGCAGCTAATATTTGATAGGATAATCAATCATGTTGTTGAACTTATTACAGACCCGTTTGGCAATTACCTTATGCAGAAGTTATTGGACATGTGTAATGAACAGCAAAGAATGCAGCTTGTACTCAAGGTGACCAAAGAACCAGGACAGCTTGCCAGAGTTTCCTTGGACACACATGGCACTCGCGTGGTTCAGAAGTTGATCGAGACTGTGGAAAATGGGAAGCAGATCTCTTTGATCATATCATCCCTTGAATGTGGCTTTCTTGATCTCATTAAGGATCCAAATGGCAATCATGTGGTACAGCGTTGCTTGGACTGCTTCAGCATTGAAGAGAACAGGTTTATTTTTGATGCTGCTGCAAGATTTTGTGTTGAGATTGCAACTCAGCGTCATGGATGTTGTGTGCTACAAAAATGCATTGCACATGCCAGTGGGAGGCATCGAGATAAGCTGGTCACTGAAGTTTCCAGGAATGGGCTTCTCCTTTCCCAAGATCCTTATGGAAACTATGTTGTACAGTATGTCATAGAGCTGAAGATCCCAGCTGTCATGGCGAGATTGATCTCTCAGTTGAAGGGACATTTTGTACTCCTCTCCATGCAGAAATGCAGTAGTCATGTAGTTGAAAAGTGCCTCAAGCACTATGAAGAGAGCCGAGTGAAGGTCATCCAAGAATTGCTCTCAGTTTCTCGCTTTGAACAGCTTTTGCAAGACCCTTATGCTAACTATGTGATTCAATCTGCTCTTTCAGTTACCAAGGGCCCTCTTCATGCTGCACTGGTTGAAGCGGTTAAGCCTCATACAATCCTACGCCACAGCCCATACTGCAAGAGGATTTTCACAAAAGACCTCCTGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

750

Amino Acids

83.56

Weight (kDa)

7.35

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 437 - 470 5.4e-11 Pumilio-family RNA binding repeat
PUF PF00806 472 - 505 1.4e-09 Pumilio-family RNA binding repeat
PUF PF00806 549 - 579 1.1e-07 Pumilio-family RNA binding repeat
PUF PF00806 585 - 617 7.8e-13 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 662 - 727 4.9e-06 NOP9-like PUF repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012349)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22240 AT1G78160 AT1G78160
fragaria_vesca FvH4_5g37620
malus_domestica MD08G1181900.v1.1 MD15G1367200.v1.1
prunus_persica Prupe.1G515100_v2.0.a1
pyrus_communis pycom08g15510 pycom15g32920
rosa_chinensis RchiOBHm_Chr7g0239301
rosa_laevigata RLG00000000765
rosa_multiflora Rmu_sc0007840.1_g000004
rosa_roxburghii Rroxscaffold_3G00221040
rosa_rugosa Rorug07G0327100
rosa_samantha Rh7AG480500 Rh7BG453200 Rh7CG498400 Rh7DG466500
rosa_wichuraiana Rw7G040250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 29
AatII GACGTC 1 cut(s) 1299
AccB1I GGYRCC 1 cut(s) 1041
AccII CGCG 1 cut(s) 1569
AciI CCGC 3 cut(s) 906, 1342, 2174
AclWI GGATC 5 cut(s) 1335, 1658, 1671, 1874, 1918
AcsI RAATTY 2 cut(s) 558, 866
AcuI CTGAAG 4 cut(s) 104, 1690, 1866, 1940
AcyI GRCGYC 1 cut(s) 1296
AfaI GTAC 4 cut(s) 1506, 1686, 1902, 1974
AfeI AGCGCT 1 cut(s) 1189
AfiI CCNNNNNNNGG 6 cut(s) 268, 1026, 1324, 1821, 1860, 2047
AflIII ACRYGT 2 cut(s) 771, 1473
AjnI CCWGG 5 cut(s) 475, 845, 1299, 1525, 1853
AjuI GAANNNNNNNTTGG 2 cut(s) 660, 692
AleI CACNNNNGTG 1 cut(s) 1568
Alw21I GWGCWC 1 cut(s) 935
Alw26I GTCTC 3 cut(s) 366, 465, 1583
AlwI GGATC 5 cut(s) 1335, 1658, 1671, 1874, 1918
Aor51HI AGCGCT 1 cut(s) 1189
AoxI GGCC 3 cut(s) 662, 848, 2146
ApaI GGGCCC 1 cut(s) 2150
ApeKI GCWGC 8 cut(s) 712, 715, 718, 1387, 1498, 1739, 1742, 2159
ApoI RAATTY 2 cut(s) 558, 866
Asp700I GAANNNNTTC 2 cut(s) 870, 895
AspLEI GCGC 1 cut(s) 1190
AspS9I GGNCC 4 cut(s) 662, 740, 2146, 2147
AsuHPI GGTGA 6 cut(s) 75, 101, 150, 692, 1073, 1525
AvaII GGWCC 1 cut(s) 740
BaeGI GKGCMC 2 cut(s) 1044, 2150
BamHI GGATCC 1 cut(s) 1663
BanI GGYRCC 1 cut(s) 1041
BanII GRGCYC 2 cut(s) 935, 2150
BbsI GAAGAC 1 cut(s) 1116
Bbv12I GWGCWC 1 cut(s) 935
BbvI GCAGC 8 cut(s) 724, 727, 730, 1399, 1510, 1726, 1729, 2146
BccI CCATC 1 cut(s) 1153
BciT130I CCWGG 5 cut(s) 477, 847, 1301, 1527, 1855
BciVI GTATCC 2 cut(s) 933, 1298
BclI TGATCA 1 cut(s) 1620
BcoDI GTCTC 3 cut(s) 366, 465, 1583
BfaI CTAG 7 cut(s) 564, 723, 1011, 1026, 1046, 1191, 1196
BfoI RGCGCY 1 cut(s) 1191
BfuI GTATCC 2 cut(s) 933, 1298
BglII AGATCT 1 cut(s) 1611
BisI GCNGC 9 cut(s) 713, 716, 719, 1342, 1388, 1499, 1740, 1743, 2160
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 9 cut(s) 714, 717, 720, 1343, 1389, 1500, 1741, 1744, 2161
Bme1390I CCNGG 5 cut(s) 477, 847, 1301, 1527, 1855
Bme18I GGWCC 1 cut(s) 740
BmgT120I GGNCC 4 cut(s) 662, 740, 2146, 2147
BmiI GGNNCC 8 cut(s) 642, 663, 742, 1018, 1043, 1372, 1665, 2148
BmrFI CCNGG 5 cut(s) 477, 847, 1301, 1527, 1855
BmrI ACTGGG 1 cut(s) 738
BmsI GCATC 4 cut(s) 142, 359, 1726, 1834
BmuI ACTGGG 1 cut(s) 738
BoxI GACNNNNGTC 1 cut(s) 1295
BpiI GAAGAC 1 cut(s) 1116
BplI GAGNNNNNCTC 2 cut(s) 922, 954
Bpu1102I GCTNAGC 1 cut(s) 15
BpuEI CTTGAG 2 cut(s) 1493, 2006
BsaHI GRCGYC 1 cut(s) 1296
BsaI GGTCTC 1 cut(s) 465
BsaJI CCNNGG 5 cut(s) 475, 527, 1300, 1548, 2142
BsaXI ACNNNNNCTCC 4 cut(s) 263, 293, 632, 662
Bsc4I CCNNNNNNNGG 6 cut(s) 268, 1026, 1324, 1821, 1860, 2047
Bse1I ACTGG 4 cut(s) 744, 992, 1815, 2169
Bse3DI GCAATG 2 cut(s) 1170, 1803
BseBI CCWGG 5 cut(s) 477, 847, 1301, 1527, 1855
BseDI CCNNGG 5 cut(s) 475, 527, 1300, 1548, 2142
BseGI GGATG 6 cut(s) 391, 436, 988, 1628, 1787, 2052
BseLI CCNNNNNNNGG 6 cut(s) 268, 1026, 1324, 1821, 1860, 2047
BseMI GCAATG 2 cut(s) 1170, 1803
BseNI ACTGG 4 cut(s) 744, 992, 1815, 2169
BseRI GAGGAG 3 cut(s) 327, 330, 1967
BseSI GKGCMC 2 cut(s) 1044, 2150
BseXI GCAGC 8 cut(s) 724, 727, 730, 1399, 1510, 1726, 1729, 2146
BseYI CCCAGC 1 cut(s) 1927
BsgI GTGCAG 3 cut(s) 1056, 1406, 2145
Bsh1236I CGCG 1 cut(s) 1569
BshFI GGCC 3 cut(s) 664, 850, 2148
BshNI GGYRCC 1 cut(s) 1041
BsiHKAI GWGCWC 1 cut(s) 935
BslFI GGGAC 3 cut(s) 706, 726, 1977
BslI CCNNNNNNNGG 6 cut(s) 268, 1026, 1324, 1821, 1860, 2047
BsmAI GTCTC 3 cut(s) 366, 465, 1583
BsmFI GGGAC 3 cut(s) 706, 726, 1977
BsmI GAATGC 2 cut(s) 1362, 1500
BsnI GGCC 3 cut(s) 664, 850, 2148
Bso31I GGTCTC 1 cut(s) 465
Bsp120I GGGCCC 1 cut(s) 2146
Bsp1286I GDGCHC 3 cut(s) 935, 1044, 2150
Bsp1407I TGTACA 1 cut(s) 1900
Bsp1720I GCTNAGC 1 cut(s) 15
BspACI CCGC 3 cut(s) 906, 1342, 2174
BspANI GGCC 3 cut(s) 664, 850, 2148
BspFNI CGCG 1 cut(s) 1569
BspLI GGNNCC 8 cut(s) 642, 663, 742, 1018, 1043, 1372, 1665, 2148
BspPI GGATC 5 cut(s) 1335, 1658, 1671, 1874, 1918
BspT107I GGYRCC 1 cut(s) 1041
BspTNI GGTCTC 1 cut(s) 465
BsrDI GCAATG 2 cut(s) 1170, 1803
BsrGI TGTACA 1 cut(s) 1900
BsrI ACTGG 4 cut(s) 744, 992, 1815, 2169
BssECI CCNNGG 5 cut(s) 475, 527, 1300, 1548, 2142
BssNI GRCGYC 1 cut(s) 1296
BssT1I CCWWGG 3 cut(s) 527, 1548, 2142
Bst2UI CCWGG 5 cut(s) 477, 847, 1301, 1527, 1855
Bst4CI ACNGT 5 cut(s) 697, 882, 1337, 1594, 1905
Bst6I CTCTTC 3 cut(s) 1710, 2028, 2157
BstACI GRCGYC 1 cut(s) 1296
BstAPI GCANNNNNTGC 1 cut(s) 1495
BstAUI TGTACA 1 cut(s) 1900
BstC8I GCNNGC 2 cut(s) 342, 1536
BstEII GGTNACC 1 cut(s) 1513
BstENI CCTNNNNNAGG 1 cut(s) 1322
BstF5I GGATG 6 cut(s) 391, 436, 988, 1628, 1787, 2052
BstFNI CGCG 1 cut(s) 1569
BstH2I RGCGCY 1 cut(s) 1191
BstHHI GCGC 1 cut(s) 1190
BstMAI GTCTC 3 cut(s) 366, 465, 1583
BstMWI GCNNNNNNNGC 6 cut(s) 130, 718, 1495, 1701, 1937, 2211
BstNI CCWGG 5 cut(s) 477, 847, 1301, 1527, 1855
BstNSI RCATGY 3 cut(s) 775, 1477, 1814
BstPAI GACNNNNGTC 1 cut(s) 1295
BstPI GGTNACC 1 cut(s) 1513
BstSCI CCNGG 5 cut(s) 475, 845, 1299, 1525, 1853
BstSLI GKGCMC 2 cut(s) 1044, 2150
BstUI CGCG 1 cut(s) 1569
BstV1I GCAGC 8 cut(s) 724, 727, 730, 1399, 1510, 1726, 1729, 2146
BstV2I GAAGAC 1 cut(s) 1116
BstX2I RGATCY 4 cut(s) 1611, 1663, 1879, 1923
BstXI CCANNNNNNTGG 1 cut(s) 149
BstYI RGATCY 4 cut(s) 1611, 1663, 1879, 1923
BsuI GTATCC 2 cut(s) 933, 1298
BsuRI GGCC 3 cut(s) 664, 850, 2148
BtsCI GGATG 6 cut(s) 391, 436, 988, 1628, 1787, 2052
BtsI GCAGTG 2 cut(s) 1231, 1268
BtsIMutI CAGTG 8 cut(s) 751, 999, 1231, 1266, 1268, 1822, 1842, 2162
Cac8I GCNNGC 2 cut(s) 342, 1536
CfoI GCGC 1 cut(s) 1190
Cfr13I GGNCC 4 cut(s) 662, 740, 2146, 2147
CseI GACGC 1 cut(s) 1763
Csp6I GTAC 4 cut(s) 1505, 1685, 1901, 1973
CviQI GTAC 4 cut(s) 1505, 1685, 1901, 1973
DrdI GACNNNNNNGTC 1 cut(s) 29
DseDI GACNNNNNNGTC 1 cut(s) 29
Eam1104I CTCTTC 3 cut(s) 1710, 2028, 2157
EarI CTCTTC 3 cut(s) 1710, 2028, 2157
EciI GGCGGA 1 cut(s) 921
Ecl136II GAGCTC 1 cut(s) 933
Eco130I CCWWGG 3 cut(s) 527, 1548, 2142
Eco147I AGGCCT 1 cut(s) 850
Eco24I GRGCYC 2 cut(s) 935, 2150
Eco31I GGTCTC 1 cut(s) 465
Eco47I GGWCC 1 cut(s) 740
Eco47III AGCGCT 1 cut(s) 1189
Eco53kI GAGCTC 1 cut(s) 933
Eco57I CTGAAG 4 cut(s) 104, 1690, 1866, 1940
Eco91I GGTNACC 1 cut(s) 1513
EcoICRI GAGCTC 1 cut(s) 933
EcoNI CCTNNNNNAGG 1 cut(s) 1322
EcoO109I RGGNCCY 2 cut(s) 2146, 2147
EcoO65I GGTNACC 1 cut(s) 1513
EcoRII CCWGG 5 cut(s) 475, 845, 1299, 1525, 1853
EcoT14I CCWWGG 3 cut(s) 527, 1548, 2142
EcoT22I ATGCAT 1 cut(s) 1805
EcoT38I GRGCYC 2 cut(s) 935, 2150
ErhI CCWWGG 3 cut(s) 527, 1548, 2142
FaqI GGGAC 3 cut(s) 706, 726, 1977
FauNDI CATATG 2 cut(s) 708, 918
FbaI TGATCA 1 cut(s) 1620
Fnu4HI GCNGC 9 cut(s) 713, 716, 719, 1342, 1388, 1499, 1740, 1743, 2160
FokI GGATG 6 cut(s) 398, 443, 975, 1615, 1794, 2039
FriOI GRGCYC 2 cut(s) 935, 2150
Fsp4HI GCNGC 9 cut(s) 713, 716, 719, 1342, 1388, 1499, 1740, 1743, 2160
FspBI CTAG 7 cut(s) 564, 723, 1011, 1026, 1046, 1191, 1196
GlaI GCGC 1 cut(s) 1189
GluI GCNGC 9 cut(s) 713, 716, 719, 1342, 1388, 1499, 1740, 1743, 2160
GsaI CCCAGC 1 cut(s) 1931
HaeII RGCGCY 1 cut(s) 1191
HaeIII GGCC 3 cut(s) 664, 850, 2148
HgaI GACGC 1 cut(s) 1763
HhaI GCGC 1 cut(s) 1190
Hin1I GRCGYC 1 cut(s) 1296
Hin6I GCGC 1 cut(s) 1188
HinP1I GCGC 1 cut(s) 1188
HinfI GANTC 8 cut(s) 332, 359, 683, 896, 1096, 1205, 1256, 2119
HphI GGTGA 6 cut(s) 75, 101, 150, 692, 1073, 1525
Hpy166II GTNNAC 3 cut(s) 970, 980, 1088
Hpy188I TCNGA 6 cut(s) 187, 285, 580, 927, 1245, 1578
Hpy188III TCNNGA 6 cut(s) 34, 1379, 1588, 1649, 1829, 2239
Hpy8I GTNNAC 3 cut(s) 970, 980, 1088
HpyAV CCTTC 8 cut(s) 122, 207, 264, 447, 581, 1169, 1954, 2041
HpyCH4III ACNGT 5 cut(s) 697, 882, 1337, 1594, 1905
HpyCH4IV ACGT 1 cut(s) 1296
HpyF10VI GCNNNNNNNGC 6 cut(s) 130, 718, 1495, 1701, 1937, 2211
HpySE526I ACGT 1 cut(s) 1296
Hsp92I GRCGYC 1 cut(s) 1296
HspAI GCGC 1 cut(s) 1188
Ksp22I TGATCA 1 cut(s) 1620
LmnI GCTCC 3 cut(s) 640, 778, 938
Lsp1109I GCAGC 8 cut(s) 724, 727, 730, 1399, 1510, 1726, 1729, 2146
LweI GCATC 4 cut(s) 142, 359, 1726, 1834
MaeI CTAG 7 cut(s) 564, 723, 1011, 1026, 1046, 1191, 1196
MaeII ACGT 1 cut(s) 1296
MaeIII GTNAC 6 cut(s) 767, 1105, 1266, 1513, 1840, 2137
MboII GAAGA 8 cut(s) 147, 766, 1116, 1153, 1727, 1933, 2045, 2144
MflI RGATCY 4 cut(s) 1611, 1663, 1879, 1923
MhlI GDGCHC 3 cut(s) 935, 1044, 2150
MluCI AATT 6 cut(s) 8, 94, 558, 866, 1447, 2060
MlyI GAGTC 3 cut(s) 326, 368, 1250
MmeI TCCRAC 2 cut(s) 309, 756
Mph1103I ATGCAT 1 cut(s) 1805
MroXI GAANNNNTTC 2 cut(s) 870, 895
MseI TTAA 5 cut(s) 5, 627, 702, 1659, 2178
MslI CAYNNNNRTG 9 cut(s) 141, 147, 369, 441, 609, 883, 1157, 1568, 1815
MspA1I CMGCKG 1 cut(s) 1931
MspR9I CCNGG 5 cut(s) 477, 847, 1301, 1527, 1855
Mva1269I GAATGC 2 cut(s) 1362, 1500
MvaI CCWGG 5 cut(s) 477, 847, 1301, 1527, 1855
MvnI CGCG 1 cut(s) 1569
MwoI GCNNNNNNNGC 6 cut(s) 130, 718, 1495, 1701, 1937, 2211
NdeI CATATG 2 cut(s) 708, 918
NlaIV GGNNCC 8 cut(s) 642, 663, 742, 1018, 1043, 1372, 1665, 2148
NmeAIII GCCGAG 1 cut(s) 2066
NmuCI GTSAC 4 cut(s) 1105, 1266, 1513, 1840
NsiI ATGCAT 1 cut(s) 1805
NspI RCATGY 3 cut(s) 775, 1477, 1814
OliI CACNNNNGTG 1 cut(s) 1568
PceI AGGCCT 1 cut(s) 850
PciI ACATGT 2 cut(s) 771, 1473
PctI GAATGC 2 cut(s) 1362, 1500
PdmI GAANNNNTTC 2 cut(s) 870, 895
PfeI GAWTC 5 cut(s) 683, 896, 1096, 1205, 2119
PfoI TCCNGGA 1 cut(s) 1853
PkrI GCNGC 9 cut(s) 714, 717, 720, 1343, 1389, 1500, 1741, 1744, 2161
PleI GAGTC 3 cut(s) 326, 367, 1250
PpsI GAGTC 3 cut(s) 326, 367, 1250
PscI ACATGT 2 cut(s) 771, 1473
PshAI GACNNNNGTC 1 cut(s) 1295
Psp124BI GAGCTC 1 cut(s) 935
Psp6I CCWGG 5 cut(s) 475, 845, 1299, 1525, 1853
PspEI GGTNACC 1 cut(s) 1513
PspFI CCCAGC 1 cut(s) 1927
PspGI CCWGG 5 cut(s) 475, 845, 1299, 1525, 1853
PspN4I GGNNCC 8 cut(s) 642, 663, 742, 1018, 1043, 1372, 1665, 2148
PspOMI GGGCCC 1 cut(s) 2146
PspPI GGNCC 4 cut(s) 662, 740, 2146, 2147
PsuI RGATCY 4 cut(s) 1611, 1663, 1879, 1923
PvuII CAGCTG 1 cut(s) 1931
RsaI GTAC 4 cut(s) 1506, 1686, 1902, 1974
RsaNI GTAC 4 cut(s) 1505, 1685, 1901, 1973
RseI CAYNNNNRTG 9 cut(s) 141, 147, 369, 441, 609, 883, 1157, 1568, 1815
SacI GAGCTC 1 cut(s) 935
SaqAI TTAA 5 cut(s) 5, 627, 702, 1659, 2178
SatI GCNGC 9 cut(s) 713, 716, 719, 1342, 1388, 1499, 1740, 1743, 2160
Sau96I GGNCC 4 cut(s) 662, 740, 2146, 2147
SchI GAGTC 3 cut(s) 326, 368, 1250
ScrFI CCNGG 5 cut(s) 477, 847, 1301, 1527, 1855
SduI GDGCHC 3 cut(s) 935, 1044, 2150
SfaNI GCATC 4 cut(s) 142, 359, 1726, 1834
SinI GGWCC 1 cut(s) 740
SmiMI CAYNNNNRTG 9 cut(s) 141, 147, 369, 441, 609, 883, 1157, 1568, 1815
SmlI CTYRAG 2 cut(s) 1508, 2021
SmoI CTYRAG 2 cut(s) 1508, 2021
Sse9I AATT 6 cut(s) 8, 94, 558, 866, 1447, 2060
SseBI AGGCCT 1 cut(s) 850
SsiI CCGC 3 cut(s) 906, 1342, 2174
SspI AATATT 1 cut(s) 1395
SspMI CTAG 7 cut(s) 564, 723, 1011, 1026, 1046, 1191, 1196
SstI GAGCTC 1 cut(s) 935
StuI AGGCCT 1 cut(s) 850
StyD4I CCNGG 5 cut(s) 475, 845, 1299, 1525, 1853
StyI CCWWGG 3 cut(s) 527, 1548, 2142
TaaI ACNGT 5 cut(s) 697, 882, 1337, 1594, 1905
TaiI ACGT 1 cut(s) 1299
TaqI TCGA 3 cut(s) 1362, 1587, 1828
TasI AATT 6 cut(s) 8, 94, 558, 866, 1447, 2060
TatI WGTACW 3 cut(s) 1504, 1900, 1972
TauI GCSGC 1 cut(s) 1344
TfiI GAWTC 5 cut(s) 683, 896, 1096, 1205, 2119
Tru1I TTAA 5 cut(s) 5, 627, 702, 1659, 2178
Tru9I TTAA 5 cut(s) 5, 627, 702, 1659, 2178
TscAI CASTG 8 cut(s) 751, 999, 1231, 1266, 1275, 1822, 1849, 2169
TseFI GTSAC 4 cut(s) 1105, 1266, 1513, 1840
TseI GCWGC 8 cut(s) 712, 715, 718, 1387, 1498, 1739, 1742, 2159
Tsp45I GTSAC 4 cut(s) 1105, 1266, 1513, 1840
TspRI CASTG 8 cut(s) 751, 999, 1231, 1266, 1275, 1822, 1849, 2169
VpaK11BI GGWCC 1 cut(s) 740
XagI CCTNNNNNAGG 1 cut(s) 1322
XapI RAATTY 2 cut(s) 558, 866
XceI RCATGY 3 cut(s) 775, 1477, 1814
XcmI CCANNNNNNNNNTGG 2 cut(s) 1546, 1883
XmnI GAANNNNTTC 2 cut(s) 870, 895
XspI CTAG 7 cut(s) 564, 723, 1011, 1026, 1046, 1191, 1196
ZraI GACGTC 1 cut(s) 1297
Zsp2I ATGCAT 1 cut(s) 1805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.