Rw7G040250

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0012349
Physical Location & Seq
Reverse (-)
62384598 .. 62388670
4073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G040250.1

Sequence Viewer

Length: 2190 bp
ATGAACATGAAGAATGATAACAACAACAACAACCCAAGAATCCCTCCACCTTATTGTGATTCCAATCTTTTTCAGCTTCTGCTTCAGTCTAACCATCACCATGTGAGTGGTTCACCTATTAGCAGAGCTTCTCTGCAATCTGAGTTTTCTCCTTCGAGTTCCTTCTCAAATGGGTTTTATTCCTCTGAGGATAGCTCTCCTTATTCTCCAACCCCATTTGAGGAGGCAAAGTATCAAACACCTCTTAAGCATTACATGAATGGAGGGGGGTTGTGGTTGGACTCCAAGCCAGCACCACCACCACATGAGTCTCATTATAATGAGATTTTGGGGGATGATCTGGGGTTGGCTGAGAGTTTTTACAGGATGAATATGGGGGATGGACATGAGGGTGGTGCTAAAAGGAGGGGGTTTGAGAGAGACCCAGATGGGTTTGGGCTTGGTGGTGGTTTCTTAGGGGGTAATTTTCCTTGGAATGTTGAGAACTATGGTTTGTTTGAAACTTCTAGGAACAATATGTCTGATGTTGAAGCTTTTCAGTCCTCTCATTTTGGTGTTAGAGGTGGTTTTAATGGTTATGTGGAGCCTGAGTTTCTTGGGTTGGGGCCAGAATATGTTGTTGGTGATTCAATGGGGGCTGTTCTAAATCATATGCAGCAGCCTAGTGCTTTGTGTTGTGGTCCTAGTGGTGCTAAGAACCAAATGGGTTACATGGTAGAGCAAAGAAAGGAACTGAGAGATGATTGGTATTACAGAAATGTTCAAGTAGAGAACCCATATGTTGCCAGACCTTACCTTGATGATGAATTTGTTTATTCACAGCTATGTGGAACAGATTCTAGTGGCGGAAAGGGTCTTATGTATCCGATGAGCTCTCCTCAGTTCCCCTCAGTTGCTCAGCTGTGTGTAGACAATGGTGCACATAATCATCCAGTGATAAAACAAAGGACTAGAGGAAATCCGACTAGGAAATGTGCAGGGCACTTAGAGGGTTTTAGAGGTGAGGATAGTCTTGATGTACAAGGAAAACATTTGAACCATGGTGTCACTAAGGGTAGCAAGTCTTCAAAGGCTAACAAGAAGAACTCTTGCACTAAGATGCCAATGAGGATAGAAGGAGAGCAAAGCTCTAGATTAGATTTGGATTCATATAATGGAGGAAGTAGTGGAAATGAATGTAGTCAGAGCAGTAATGACTCAATGAAGTCGCTGCTTACTTTGGGTTCATTGACTGAGGTCCAGGGATACATATACTTCCTAGCAAAGGATCAGCATGGTTGCCGGTTCTTACAGAGAATGCTCGATGAAGGAACCCGTCAAGATGTGCAATTAATATTTGATAGGATAATCAATCATGTTCTTGAACTTATGACGGACCCGTTTGGCAATTACCTAATGCAGAAGTTCTTGGACATGTGTAATGAACAGCAAAGAATGCAGTTTGTACTTAAGGTGACCAAAGAACCGGGACAGCTTGCGAGAGTTTCCTTGGACACACATGGCACTCGTGTGGTGCAGAAGTTGATCGAGACTGTGGAAAATGGGAAACAGGTCTCCTTGATCATATCATCCCTTGAATGTGGTTTTCTTGATCTTATTAAGGATCCAAATGGCAATCATGTGGTACAGCGTTGCTTGGACTGCTTCAGCACAGAAGAGAACAGGTTTATATTTGATGCTGCTGCAAGATTCTGTGTTGAGATTGCAACTCAGCGTCATGGATGCTGTGTGCTACAAAAATGCATTGCACATGCCAGTGGGAGACATCGAGATAAGCTGGTCACTGAAGTTTCTAGGAATGGGCTTCTCCTTTCCCAAGATCCGTATGGAAACTATGTTGTTCAGTATGTCATAGAGCTGAAAATCCCATCAGTCATGGCCAGATTGATCTCTCAGTTGAAGGGACACTTTGTACTCCTCTCCATGCAGAAATGCAGTAGTCATGTTGTTGAAAAATGCCTCAAGCACTATGAAGAGAGCCGAGCAAAAGTCATCCAGGAATTGCTCTCAGTTTCTCACTTTGAACAGCTGTTGCAAGACCCTTATGCTAACTATGTCATTCAATCTGCTCTTTCAGTTACCAAGGGCCCTCTTCACGCTGCACTGGTTGAAGCTGTCAAGCCTCATACAATCTTGCGCCACAGCCCATATTGCAAGAGGATTTTCACAAAGGACCTCCTGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

729

Amino Acids

81.57

Weight (kDa)

6.64

Isoelectric Point (pI)

48.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 417 - 450 4.2e-11 Pumilio-family RNA binding repeat
PUF PF00806 452 - 484 5.7e-09 Pumilio-family RNA binding repeat
PUF PF00806 529 - 559 3.5e-08 Pumilio-family RNA binding repeat
PUF PF00806 565 - 597 7.6e-13 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012349)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22240 AT1G78160 AT1G78160
fragaria_vesca FvH4_5g37620
malus_domestica MD08G1181900.v1.1 MD15G1367200.v1.1
prunus_persica Prupe.1G515100_v2.0.a1
pyrus_communis pycom08g15510 pycom15g32920
rosa_chinensis RchiOBHm_Chr7g0239301
rosa_laevigata RLG00000000765
rosa_multiflora Rmu_sc0007840.1_g000004
rosa_roxburghii Rroxscaffold_3G00221040
rosa_rugosa Rorug07G0327100
rosa_samantha Rh7AG480500 Rh7BG453200 Rh7CG498400 Rh7DG466500
rosa_wichuraiana Rw7G040250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 318
AccI GTMKAC 1 cut(s) 909
AciI CCGC 1 cut(s) 846
AclWI GGATC 4 cut(s) 1275, 1598, 1611, 1814
AcoI YGGCCR 1 cut(s) 1878
AcsI RAATTY 1 cut(s) 806
AcuI CTGAAG 3 cut(s) 68, 1630, 1806
AdeI CACNNNGTG 1 cut(s) 103
AfaI GTAC 4 cut(s) 1020, 1446, 1626, 1914
AfiI CCNNNNNNNGG 2 cut(s) 220, 1264
AflII CTTAAG 2 cut(s) 245, 1448
AflIII ACRYGT 1 cut(s) 1413
AjnI CCWGG 2 cut(s) 1239, 1995
AjuI GAANNNNNNNTTGG 2 cut(s) 603, 635
AleI CACNNNNGTG 1 cut(s) 1508
Alw21I GWGCWC 2 cut(s) 875, 922
Alw26I GTCTC 5 cut(s) 315, 414, 1523, 1558, 1756
Alw44I GTGCAC 1 cut(s) 918
AlwI GGATC 4 cut(s) 1275, 1598, 1611, 1814
AlwNI CAGNNNCTG 1 cut(s) 79
AoxI GGCC 3 cut(s) 605, 1878, 2086
ApaI GGGCCC 1 cut(s) 2090
ApaLI GTGCAC 1 cut(s) 918
ApeKI GCWGC 6 cut(s) 655, 658, 1210, 1679, 1682, 2099
ApoI RAATTY 1 cut(s) 806
AseI ATTAAT 1 cut(s) 1331
Asp700I GAANNNNTTC 2 cut(s) 534, 835
AspLEI GCGC 1 cut(s) 2139
AspS9I GGNCC 7 cut(s) 605, 680, 1237, 1375, 2086, 2087, 2173
AsuC2I CCSGG 1 cut(s) 1467
AsuHPI GGTGA 5 cut(s) 89, 105, 635, 1013, 1465
AvaII GGWCC 4 cut(s) 680, 1237, 1375, 2173
BaeGI GKGCMC 3 cut(s) 922, 984, 2090
BalI TGGCCA 1 cut(s) 1880
BamHI GGATCC 1 cut(s) 1603
BanII GRGCYC 2 cut(s) 875, 2090
BarI GAAGNNNNNNTAC 2 cut(s) 1048, 1080
BauI CACGAG 1 cut(s) 1506
BbsI GAAGAC 1 cut(s) 1056
Bbv12I GWGCWC 2 cut(s) 875, 922
BbvI GCAGC 6 cut(s) 667, 670, 1197, 1666, 1669, 2086
BccI CCATC 4 cut(s) 102, 374, 422, 1876
BciT130I CCWGG 2 cut(s) 1241, 1997
BciVI GTATCC 2 cut(s) 873, 1238
BclI TGATCA 1 cut(s) 1560
BcnI CCSGG 1 cut(s) 1467
BcoDI GTCTC 5 cut(s) 315, 414, 1523, 1558, 1756
BfaI CTAG 9 cut(s) 507, 663, 684, 840, 951, 966, 1131, 1259, 1794
BfrI CTTAAG 2 cut(s) 245, 1448
BfuI GTATCC 2 cut(s) 873, 1238
BisI GCNGC 6 cut(s) 656, 659, 1211, 1680, 1683, 2100
BlpI GCTNAGC 1 cut(s) 897
BlsI GCNGC 6 cut(s) 657, 660, 1212, 1681, 1684, 2101
Bme1390I CCNGG 3 cut(s) 1241, 1467, 1997
Bme18I GGWCC 4 cut(s) 680, 1237, 1375, 2173
BmgT120I GGNCC 7 cut(s) 605, 680, 1237, 1375, 2086, 2087, 2173
BmiI GGNNCC 6 cut(s) 585, 606, 1312, 1377, 1605, 2088
BmrFI CCNGG 3 cut(s) 1241, 1467, 1997
BmsI GCATC 3 cut(s) 1089, 1666, 1712
BoxI GACNNNNGTC 1 cut(s) 1235
BpiI GAAGAC 1 cut(s) 1056
BplI GAGNNNNNCTC 6 cut(s) 179, 211, 862, 894, 1112, 1144
Bpu1102I GCTNAGC 1 cut(s) 897
BpuEI CTTGAG 1 cut(s) 1946
BpuMI CCSGG 1 cut(s) 1467
BsaBI GATNNNNATC 1 cut(s) 63
BsaI GGTCTC 2 cut(s) 414, 1558
BsaJI CCNNGG 5 cut(s) 470, 1039, 1240, 1488, 2082
BsaXI ACNNNNNCTCC 4 cut(s) 215, 245, 575, 605
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 1264
Bse118I RCCGGY 1 cut(s) 1281
Bse1I ACTGG 3 cut(s) 932, 1755, 2109
Bse3DI GCAATG 1 cut(s) 1743
Bse8I GATNNNNATC 1 cut(s) 63
BseBI CCWGG 2 cut(s) 1241, 1997
BseDI CCNNGG 5 cut(s) 470, 1039, 1240, 1488, 2082
BseGI GGATG 7 cut(s) 340, 372, 385, 928, 1568, 1727, 1992
BseJI GATNNNNATC 1 cut(s) 63
BseLI CCNNNNNNNGG 2 cut(s) 220, 1264
BseMI GCAATG 1 cut(s) 1743
BseNI ACTGG 3 cut(s) 932, 1755, 2109
BseRI GAGGAG 3 cut(s) 236, 867, 1907
BseSI GKGCMC 3 cut(s) 922, 984, 2090
BseXI GCAGC 6 cut(s) 667, 670, 1197, 1666, 1669, 2086
BsgI GTGCAG 3 cut(s) 996, 1535, 2085
BshFI GGCC 3 cut(s) 607, 1880, 2088
BsiHKAI GWGCWC 2 cut(s) 875, 922
BsiSI CCGG 2 cut(s) 1282, 1466
BslFI GGGAC 2 cut(s) 1482, 1917
BslI CCNNNNNNNGG 2 cut(s) 220, 1264
BsmAI GTCTC 5 cut(s) 315, 414, 1523, 1558, 1756
BsmFI GGGAC 2 cut(s) 1482, 1917
BsmI GAATGC 2 cut(s) 1302, 1440
BsnI GGCC 3 cut(s) 607, 1880, 2088
Bso31I GGTCTC 2 cut(s) 414, 1558
Bsp120I GGGCCC 1 cut(s) 2086
Bsp1286I GDGCHC 4 cut(s) 875, 922, 984, 2090
Bsp1407I TGTACA 1 cut(s) 1018
Bsp143I GATC 8 cut(s) 337, 1267, 1524, 1560, 1591, 1603, 1819, 1887
Bsp1720I GCTNAGC 1 cut(s) 897
Bsp19I CCATGG 1 cut(s) 1039
BspACI CCGC 1 cut(s) 846
BspANI GGCC 3 cut(s) 607, 1880, 2088
BspLI GGNNCC 6 cut(s) 585, 606, 1312, 1377, 1605, 2088
BspPI GGATC 4 cut(s) 1275, 1598, 1611, 1814
BspTI CTTAAG 2 cut(s) 245, 1448
BspTNI GGTCTC 2 cut(s) 414, 1558
BsrDI GCAATG 1 cut(s) 1743
BsrFI RCCGGY 1 cut(s) 1281
BsrGI TGTACA 1 cut(s) 1018
BsrI ACTGG 3 cut(s) 932, 1755, 2109
BssAI RCCGGY 1 cut(s) 1281
BssECI CCNNGG 5 cut(s) 470, 1039, 1240, 1488, 2082
BssMI GATC 8 cut(s) 337, 1267, 1524, 1560, 1591, 1603, 1819, 1887
BssSI CACGAG 1 cut(s) 1506
BssT1I CCWWGG 4 cut(s) 470, 1039, 1488, 2082
Bst2BI CACGAG 1 cut(s) 1506
Bst2UI CCWGG 2 cut(s) 1241, 1997
Bst4CI ACNGT 1 cut(s) 1534
Bst6I CTCTTC 3 cut(s) 1650, 1968, 2097
BstAFI CTTAAG 2 cut(s) 245, 1448
BstAPI GCANNNNNTGC 1 cut(s) 1435
BstAUI TGTACA 1 cut(s) 1018
BstC8I GCNNGC 2 cut(s) 291, 1476
BstDSI CCRYGG 1 cut(s) 1039
BstEII GGTNACC 1 cut(s) 1453
BstENI CCTNNNNNAGG 1 cut(s) 1262
BstF5I GGATG 7 cut(s) 340, 372, 385, 928, 1568, 1727, 1992
BstHHI GCGC 1 cut(s) 2139
BstKTI GATC 8 cut(s) 340, 1270, 1527, 1563, 1594, 1606, 1822, 1890
BstMAI GTCTC 5 cut(s) 315, 414, 1523, 1558, 1756
BstMBI GATC 8 cut(s) 337, 1267, 1524, 1560, 1591, 1603, 1819, 1887
BstMWI GCNNNNNNNGC 3 cut(s) 1435, 1641, 2151
BstNI CCWGG 2 cut(s) 1241, 1997
BstNSI RCATGY 2 cut(s) 1417, 1754
BstPAI GACNNNNGTC 1 cut(s) 1235
BstPI GGTNACC 1 cut(s) 1453
BstSCI CCNGG 3 cut(s) 1239, 1465, 1995
BstSLI GKGCMC 3 cut(s) 922, 984, 2090
BstV1I GCAGC 6 cut(s) 667, 670, 1197, 1666, 1669, 2086
BstV2I GAAGAC 1 cut(s) 1056
BstX2I RGATCY 2 cut(s) 1603, 1819
BstXI CCANNNNNNTGG 1 cut(s) 107
BstYI RGATCY 2 cut(s) 1603, 1819
BsuI GTATCC 2 cut(s) 873, 1238
BsuRI GGCC 3 cut(s) 607, 1880, 2088
BtgI CCRYGG 1 cut(s) 1039
BtsCI GGATG 7 cut(s) 340, 372, 385, 928, 1568, 1727, 1992
BtsIMutI CAGTG 4 cut(s) 939, 1762, 1782, 2102
Cac8I GCNNGC 2 cut(s) 291, 1476
CaiI CAGNNNCTG 1 cut(s) 79
CfoI GCGC 1 cut(s) 2139
Cfr10I RCCGGY 1 cut(s) 1281
Cfr13I GGNCC 7 cut(s) 605, 680, 1237, 1375, 2086, 2087, 2173
CseI GACGC 1 cut(s) 1703
Csp6I GTAC 4 cut(s) 1019, 1445, 1625, 1913
CviQI GTAC 4 cut(s) 1019, 1445, 1625, 1913
DpnI GATC 8 cut(s) 339, 1269, 1526, 1562, 1593, 1605, 1821, 1889
DpnII GATC 8 cut(s) 337, 1267, 1524, 1560, 1591, 1603, 1819, 1887
DraIII CACNNNGTG 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 1878
Eam1104I CTCTTC 3 cut(s) 1650, 1968, 2097
EarI CTCTTC 3 cut(s) 1650, 1968, 2097
EciI GGCGGA 1 cut(s) 861
Ecl136II GAGCTC 1 cut(s) 873
Eco130I CCWWGG 4 cut(s) 470, 1039, 1488, 2082
Eco24I GRGCYC 2 cut(s) 875, 2090
Eco31I GGTCTC 2 cut(s) 414, 1558
Eco47I GGWCC 4 cut(s) 680, 1237, 1375, 2173
Eco53kI GAGCTC 1 cut(s) 873
Eco57I CTGAAG 3 cut(s) 68, 1630, 1806
Eco91I GGTNACC 1 cut(s) 1453
EcoICRI GAGCTC 1 cut(s) 873
EcoNI CCTNNNNNAGG 1 cut(s) 1262
EcoO109I RGGNCCY 3 cut(s) 2086, 2087, 2173
EcoO65I GGTNACC 1 cut(s) 1453
EcoRII CCWGG 2 cut(s) 1239, 1995
EcoT14I CCWWGG 4 cut(s) 470, 1039, 1488, 2082
EcoT22I ATGCAT 1 cut(s) 1745
EcoT38I GRGCYC 2 cut(s) 875, 2090
ErhI CCWWGG 4 cut(s) 470, 1039, 1488, 2082
FalI AAGNNNNNCTT 2 cut(s) 1892, 1924
FaqI GGGAC 2 cut(s) 1482, 1917
FauNDI CATATG 2 cut(s) 651, 778
FbaI TGATCA 1 cut(s) 1560
FblI GTMKAC 1 cut(s) 909
Fnu4HI GCNGC 6 cut(s) 656, 659, 1211, 1680, 1683, 2100
FokI GGATG 7 cut(s) 347, 379, 392, 915, 1555, 1734, 1979
FriOI GRGCYC 2 cut(s) 875, 2090
Fsp4HI GCNGC 6 cut(s) 656, 659, 1211, 1680, 1683, 2100
FspBI CTAG 9 cut(s) 507, 663, 684, 840, 951, 966, 1131, 1259, 1794
GlaI GCGC 1 cut(s) 2138
GluI GCNGC 6 cut(s) 656, 659, 1211, 1680, 1683, 2100
HaeIII GGCC 3 cut(s) 607, 1880, 2088
HapII CCGG 2 cut(s) 1282, 1466
HgaI GACGC 1 cut(s) 1703
HhaI GCGC 1 cut(s) 2139
Hin6I GCGC 1 cut(s) 2137
HinP1I GCGC 1 cut(s) 2137
HindIII AAGCTT 1 cut(s) 531
HinfI GANTC 9 cut(s) 39, 59, 281, 308, 626, 836, 1145, 1196, 1689
HpaII CCGG 2 cut(s) 1282, 1466
HphI GGTGA 5 cut(s) 89, 105, 635, 1013, 1465
Hpy166II GTNNAC 3 cut(s) 113, 910, 920
Hpy188I TCNGA 6 cut(s) 142, 187, 523, 867, 963, 1185
Hpy188III TCNNGA 8 cut(s) 1013, 1131, 1319, 1361, 1528, 1589, 1769, 2179
Hpy8I GTNNAC 3 cut(s) 113, 910, 920
HpyAV CCTTC 5 cut(s) 162, 172, 1109, 1301, 1894
HpyCH4III ACNGT 1 cut(s) 1534
HpyF10VI GCNNNNNNNGC 3 cut(s) 1435, 1641, 2151
HspAI GCGC 1 cut(s) 2137
Ksp22I TGATCA 1 cut(s) 1560
Kzo9I GATC 8 cut(s) 337, 1267, 1524, 1560, 1591, 1603, 1819, 1887
LmnI GCTCC 1 cut(s) 583
Lsp1109I GCAGC 6 cut(s) 667, 670, 1197, 1666, 1669, 2086
LweI GCATC 3 cut(s) 1089, 1666, 1712
MaeI CTAG 9 cut(s) 507, 663, 684, 840, 951, 966, 1131, 1259, 1794
MaeIII GTNAC 5 cut(s) 707, 1045, 1453, 1780, 2077
MalI GATC 8 cut(s) 339, 1269, 1526, 1562, 1593, 1605, 1821, 1889
MboI GATC 8 cut(s) 337, 1267, 1524, 1560, 1591, 1603, 1819, 1887
MboII GAAGA 6 cut(s) 22, 1056, 1093, 1667, 1985, 2084
MflI RGATCY 2 cut(s) 1603, 1819
MhlI GDGCHC 4 cut(s) 875, 922, 984, 2090
MlsI TGGCCA 1 cut(s) 1880
MluCI AATT 5 cut(s) 463, 806, 1328, 1387, 2000
MluNI TGGCCA 1 cut(s) 1880
MlyI GAGTC 3 cut(s) 275, 317, 1190
MmeI TCCRAC 3 cut(s) 233, 258, 986
Mox20I TGGCCA 1 cut(s) 1880
Mph1103I ATGCAT 1 cut(s) 1745
MroXI GAANNNNTTC 2 cut(s) 534, 835
MscI TGGCCA 1 cut(s) 1880
MseI TTAA 5 cut(s) 246, 570, 1331, 1449, 1599
MslI CAYNNNNRTG 9 cut(s) 99, 105, 318, 390, 552, 823, 1097, 1508, 1755
Msp20I TGGCCA 1 cut(s) 1880
MspA1I CMGCKG 2 cut(s) 901, 2029
MspCI CTTAAG 2 cut(s) 245, 1448
MspI CCGG 2 cut(s) 1282, 1466
MspR9I CCNGG 3 cut(s) 1241, 1467, 1997
Mva1269I GAATGC 2 cut(s) 1302, 1440
MvaI CCWGG 2 cut(s) 1241, 1997
MwoI GCNNNNNNNGC 3 cut(s) 1435, 1641, 2151
NciI CCSGG 1 cut(s) 1467
NcoI CCATGG 1 cut(s) 1039
NdeI CATATG 2 cut(s) 651, 778
NdeII GATC 8 cut(s) 337, 1267, 1524, 1560, 1591, 1603, 1819, 1887
NlaIV GGNNCC 6 cut(s) 585, 606, 1312, 1377, 1605, 2088
NmeAIII GCCGAG 1 cut(s) 2006
NmuCI GTSAC 3 cut(s) 1045, 1453, 1780
NsiI ATGCAT 1 cut(s) 1745
NspI RCATGY 2 cut(s) 1417, 1754
OliI CACNNNNGTG 1 cut(s) 1508
PciI ACATGT 1 cut(s) 1413
PctI GAATGC 2 cut(s) 1302, 1440
PdmI GAANNNNTTC 2 cut(s) 534, 835
PfeI GAWTC 6 cut(s) 39, 59, 626, 836, 1145, 1689
PfoI TCCNGGA 1 cut(s) 1995
PkrI GCNGC 6 cut(s) 657, 660, 1212, 1681, 1684, 2101
PleI GAGTC 3 cut(s) 275, 316, 1190
PpsI GAGTC 3 cut(s) 275, 316, 1190
PpuMI RGGWCCY 1 cut(s) 2173
PscI ACATGT 1 cut(s) 1413
PshAI GACNNNNGTC 1 cut(s) 1235
PshBI ATTAAT 1 cut(s) 1331
PsiI TTATAA 1 cut(s) 318
Psp124BI GAGCTC 1 cut(s) 875
Psp5II RGGWCCY 1 cut(s) 2173
Psp6I CCWGG 2 cut(s) 1239, 1995
PspEI GGTNACC 1 cut(s) 1453
PspGI CCWGG 2 cut(s) 1239, 1995
PspN4I GGNNCC 6 cut(s) 585, 606, 1312, 1377, 1605, 2088
PspOMI GGGCCC 1 cut(s) 2086
PspPI GGNCC 7 cut(s) 605, 680, 1237, 1375, 2086, 2087, 2173
PspPPI RGGWCCY 1 cut(s) 2173
PstNI CAGNNNCTG 1 cut(s) 79
PsuI RGATCY 2 cut(s) 1603, 1819
PvuII CAGCTG 2 cut(s) 901, 2029
RsaI GTAC 4 cut(s) 1020, 1446, 1626, 1914
RsaNI GTAC 4 cut(s) 1019, 1445, 1625, 1913
RseI CAYNNNNRTG 9 cut(s) 99, 105, 318, 390, 552, 823, 1097, 1508, 1755
SacI GAGCTC 1 cut(s) 875
SaqAI TTAA 5 cut(s) 246, 570, 1331, 1449, 1599
SatI GCNGC 6 cut(s) 656, 659, 1211, 1680, 1683, 2100
Sau3AI GATC 8 cut(s) 337, 1267, 1524, 1560, 1591, 1603, 1819, 1887
Sau96I GGNCC 7 cut(s) 605, 680, 1237, 1375, 2086, 2087, 2173
SchI GAGTC 3 cut(s) 275, 317, 1190
ScrFI CCNGG 3 cut(s) 1241, 1467, 1997
SduI GDGCHC 4 cut(s) 875, 922, 984, 2090
SfaNI GCATC 3 cut(s) 1089, 1666, 1712
SinI GGWCC 4 cut(s) 680, 1237, 1375, 2173
SmiMI CAYNNNNRTG 9 cut(s) 99, 105, 318, 390, 552, 823, 1097, 1508, 1755
SmlI CTYRAG 3 cut(s) 245, 1448, 1961
SmoI CTYRAG 3 cut(s) 245, 1448, 1961
Sse9I AATT 5 cut(s) 463, 806, 1328, 1387, 2000
SsiI CCGC 1 cut(s) 846
SspI AATATT 1 cut(s) 1335
SspMI CTAG 9 cut(s) 507, 663, 684, 840, 951, 966, 1131, 1259, 1794
SstI GAGCTC 1 cut(s) 875
StyD4I CCNGG 3 cut(s) 1239, 1465, 1995
StyI CCWWGG 4 cut(s) 470, 1039, 1488, 2082
TaaI ACNGT 1 cut(s) 1534
TaqI TCGA 4 cut(s) 155, 1302, 1527, 1768
TasI AATT 5 cut(s) 463, 806, 1328, 1387, 2000
TatI WGTACW 3 cut(s) 1018, 1444, 1912
TfiI GAWTC 6 cut(s) 39, 59, 626, 836, 1145, 1689
Tru1I TTAA 5 cut(s) 246, 570, 1331, 1449, 1599
Tru9I TTAA 5 cut(s) 246, 570, 1331, 1449, 1599
TscAI CASTG 4 cut(s) 939, 1762, 1789, 2109
TseFI GTSAC 3 cut(s) 1045, 1453, 1780
TseI GCWGC 6 cut(s) 655, 658, 1210, 1679, 1682, 2099
Tsp45I GTSAC 3 cut(s) 1045, 1453, 1780
TspGWI ACGGA 2 cut(s) 1388, 1812
TspRI CASTG 4 cut(s) 939, 1762, 1789, 2109
Vha464I CTTAAG 2 cut(s) 245, 1448
VneI GTGCAC 1 cut(s) 918
VpaK11BI GGWCC 4 cut(s) 680, 1237, 1375, 2173
VspI ATTAAT 1 cut(s) 1331
XagI CCTNNNNNAGG 1 cut(s) 1262
XapI RAATTY 1 cut(s) 806
XbaI TCTAGA 1 cut(s) 1130
XceI RCATGY 2 cut(s) 1417, 1754
XcmI CCANNNNNNNNNTGG 1 cut(s) 1823
XmiI GTMKAC 1 cut(s) 909
XmnI GAANNNNTTC 2 cut(s) 534, 835
XspI CTAG 9 cut(s) 507, 663, 684, 840, 951, 966, 1131, 1259, 1794
Zsp2I ATGCAT 1 cut(s) 1745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.