Rh7BG453200

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0012349
Physical Location & Seq
Reverse (-)
53362060 .. 53367009
4950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG453200.1

Sequence Viewer

Length: 2217 bp
ATGTTGAGAATAGCTGAGCAAGACCAGAGGGTTTTGATGAACATGAAGAATGATAACAACAACAACCCAAGAATCCCTCCACCTTATTGTGATTCCAATCTTTTTCAGCTTCTGCTTCAGTCTAACCATCACCATGTGAGTGGTTCACCTATTAGCAGAGCTTCTCTGCAATCTGAGTTTTCTCCTTCTAGTTCCTTCTCAAATGGGTTTTATTCCTCTGAGGATAGCTCTCCTTATTCTCCAATCCCATTTGAGGAGGCAAAGTATCAAACACCTCTTAAGCATTACATGAATGGAGGAGGGTTGTGGTTGGACTCCAAGCCAGCACCACACCATGAGTCTCATTATAATGAGATTTTGGGGGATGATCTGGGGTTGGCTGAGAGTTTTTACAGGATGAATATGGGGGATGGACATGAGGGTGGTGCTAAAAGGAGGGGGTTTGAGAGAGACCCAGATGGGTTTGGGCTTGGTGGTGGTTTCCTAGGGGGTAATATTCCTTGGAATGTTGAGAACTATGGCTTGTTTGAAACTTCTAGGAACAATATGTCTGATGTTGAAGCTTTTCAGTCCTCTCATTTTGGTGTTAGAGGTGGGTTTAATGGTTATGTGGAGCCTGAGTTTCTTGGGTTGGGGCCAGAATATGTTGTTGGTGATTCAATGGGGGCTGTTCTAAATCATATGCAGCAGCCTAGTGCTTTGTGTTGTGGTCCTAGTGGTGCTAAGAACCAAATGGGTTACATGGTAGAGCAAAGAAAGGAACTGAGAGATGATTGGTATTACAGAAATGTTCAAGTAGAGAATCCATATGTTGCCAGACCTTACCTTGATGATGAATTTGTTTATTCACAGCTATGTGGAACAGATTCTAGTGCCGGAAAGGGTCTTATGTATCGGATGAGCCCTCAGTTCCCCTCAGTTGCTCAGCTGTGTGTGGACAATGGTTCACATAATCATTCAGTGATAAGACAAAGGACTAGAGGAAACCCAACAGGGAAATGTGCAGGGCACGTAGAGGGTTTTAGGGGTGAGGATAGTCTTGCTGTACAAGGAAAACATTTGAACCATGGTGTCACTAAGGGTGGCAAGTCTTCAAAGGCTAACAAGAAGAACTCTTGCACTAAGATGCCAATGAGGACAGAAGGAGAGAAAAGCTCTAGACTAGATTTGGATTCATATAATGGAGGAAGTAGTGGAAATGAATGTAGTCAGAGCAGTAATGACTCAATGAAGTCACTGCTTACTTTGGGTTCATTGACTGAGGTCCAGGGATACATATACTTCCTAGCAAAGGATCAGCATGGTTGCCGGTTCTTACAGAGAATGCTCGATGAGGGAACCCTTCAAGATGTGCAACTAATATTTGATAGGATAATCAATCATGTTCTTGAACTTATGACAGACCCGTTTGGCAATTACCTAATGCAGAAGTTCTTGGACATGTGTAATGAACAGCAAAGAATGCAATTTGTACTTAAGGTGACCAAAGAACCGGGACAGCTTGTGAGAGTTTCCTTGGACACACATGGCACTCGTGTGGTGCAGAAGTTGATCGAGACTGTGGAAAATGGGAAACAGGTCTCTTTGATCATATCATCCCTTGAATGTGGTTTTCTTGATCTTATTAAGGATCCAAATGGCAATCATGTGGTACAGCGTTGCTTGGACTGCTTCAGCACTGAAGAGAACAGGTTTATATTTGATGCTGCTGCAAGATTCTGTGTTGAGATTGCAACTCAGCGTCATGGATGCTGTGTGCTACAAAAATGCATTGCACATGCCAGTGGGAGACATCGAGATAAGCTGGTCACTGAAGTTTCCAGGAATGGGCTTCTCCTTTCCCAAGATCCGTATGGAAACTATGTAGTTCAGTATGTCATAGAGCTGAAGATCCCATCTGTCATGGCCCGATTGATCTCTCAGTTGAAGGGGCACTTTGTACTTCTCTCCATGCAGAAATGCAGTAGTCATGTTGTTGAAAAATGCCTCAAGCACTATGAAGAGAGCCGAGCAAAAGTCATCCAGGAATTGCTCTCAGTTTCTCACTTTGAACAGCTGTTGCAAGACCCTTATGCTAACTATGTCATTCAATCTGCTCTTTCAGTTACCAAGGGCCCTCTTCACGCTGCACTGGTTGAAGCTGTCAAGCCTCATACAATCTTGCGCCACAGCCCATACTGCAAGAGGATTTTCACAAAGGACCTCCTGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

738

Amino Acids

82.64

Weight (kDa)

6.75

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 426 - 459 7e-11 Pumilio-family RNA binding repeat
PUF PF00806 461 - 493 5.7e-09 Pumilio-family RNA binding repeat
PUF PF00806 538 - 568 3.5e-08 Pumilio-family RNA binding repeat
PUF PF00806 574 - 606 7.7e-13 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012349)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22240 AT1G78160 AT1G78160
fragaria_vesca FvH4_5g37620
malus_domestica MD08G1181900.v1.1 MD15G1367200.v1.1
prunus_persica Prupe.1G515100_v2.0.a1
pyrus_communis pycom08g15510 pycom15g32920
rosa_chinensis RchiOBHm_Chr7g0239301
rosa_laevigata RLG00000000765
rosa_multiflora Rmu_sc0007840.1_g000004
rosa_roxburghii Rroxscaffold_3G00221040
rosa_rugosa Rorug07G0327100
rosa_samantha Rh7AG480500 Rh7BG453200 Rh7CG498400 Rh7DG466500
rosa_wichuraiana Rw7G040250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 348
AclWI GGATC 5 cut(s) 1302, 1625, 1638, 1841, 1885
AcsI RAATTY 1 cut(s) 836
AcuI CTGAAG 5 cut(s) 101, 1657, 1701, 1833, 1907
AdeI CACNNNGTG 1 cut(s) 136
AfaI GTAC 4 cut(s) 1047, 1473, 1653, 1941
AfiI CCNNNNNNNGG 3 cut(s) 253, 1291, 1827
AflII CTTAAG 2 cut(s) 278, 1475
AflIII ACRYGT 1 cut(s) 1440
AjnI CCWGG 3 cut(s) 1266, 1820, 2022
AjuI GAANNNNNNNTTGG 2 cut(s) 633, 665
AleI CACNNNNGTG 1 cut(s) 1535
Alw26I GTCTC 5 cut(s) 345, 444, 1550, 1585, 1783
AlwI GGATC 5 cut(s) 1302, 1625, 1638, 1841, 1885
AlwNI CAGNNNCTG 1 cut(s) 112
AoxI GGCC 3 cut(s) 635, 1905, 2113
ApaI GGGCCC 1 cut(s) 2117
ApeKI GCWGC 5 cut(s) 685, 688, 1706, 1709, 2126
ApoI RAATTY 1 cut(s) 836
Asp700I GAANNNNTTC 2 cut(s) 564, 865
AspA2I CCTAGG 1 cut(s) 484
AspLEI GCGC 1 cut(s) 2166
AspS9I GGNCC 7 cut(s) 635, 710, 1264, 1906, 2113, 2114, 2200
AsuC2I CCSGG 1 cut(s) 1494
AsuHPI GGTGA 5 cut(s) 122, 138, 665, 1040, 1492
AvaII GGWCC 3 cut(s) 710, 1264, 2200
AvrII CCTAGG 1 cut(s) 484
BaeGI GKGCMC 3 cut(s) 1011, 1935, 2117
BamHI GGATCC 1 cut(s) 1630
BanII GRGCYC 2 cut(s) 905, 2117
BauI CACGAG 1 cut(s) 1533
BbsI GAAGAC 1 cut(s) 1083
BbvI GCAGC 5 cut(s) 697, 700, 1693, 1696, 2113
BccI CCATC 4 cut(s) 135, 404, 452, 1903
BciT130I CCWGG 3 cut(s) 1268, 1822, 2024
BciVI GTATCC 1 cut(s) 1265
BclI TGATCA 1 cut(s) 1587
BcnI CCSGG 1 cut(s) 1494
BcoDI GTCTC 5 cut(s) 345, 444, 1550, 1585, 1783
BfrI CTTAAG 2 cut(s) 278, 1475
BfuI GTATCC 1 cut(s) 1265
BisI GCNGC 5 cut(s) 686, 689, 1707, 1710, 2127
BlnI CCTAGG 1 cut(s) 484
BlpI GCTNAGC 2 cut(s) 15, 924
BlsI GCNGC 5 cut(s) 687, 690, 1708, 1711, 2128
Bme1390I CCNGG 4 cut(s) 1268, 1494, 1822, 2024
Bme18I GGWCC 3 cut(s) 710, 1264, 2200
BmgT120I GGNCC 7 cut(s) 635, 710, 1264, 1906, 2113, 2114, 2200
BmiI GGNNCC 5 cut(s) 615, 636, 1339, 1632, 2115
BmrFI CCNGG 4 cut(s) 1268, 1494, 1822, 2024
BmsI GCATC 3 cut(s) 1116, 1693, 1739
BoxI GACNNNNGTC 1 cut(s) 1262
BpiI GAAGAC 1 cut(s) 1083
BplI GAGNNNNNCTC 4 cut(s) 212, 244, 1139, 1171
Bpu1102I GCTNAGC 2 cut(s) 15, 924
BpuEI CTTGAG 1 cut(s) 1973
BpuMI CCSGG 1 cut(s) 1494
BsaAI YACGTR 1 cut(s) 1012
BsaBI GATNNNNATC 1 cut(s) 96
BsaI GGTCTC 2 cut(s) 444, 1585
BsaJI CCNNGG 6 cut(s) 484, 500, 1066, 1267, 1515, 2109
BsaXI ACNNNNNCTCC 4 cut(s) 248, 278, 605, 635
Bsc4I CCNNNNNNNGG 3 cut(s) 253, 1291, 1827
Bse118I RCCGGY 1 cut(s) 1308
Bse1I ACTGG 2 cut(s) 1782, 2136
Bse3DI GCAATG 1 cut(s) 1770
Bse8I GATNNNNATC 1 cut(s) 96
BseBI CCWGG 3 cut(s) 1268, 1822, 2024
BseDI CCNNGG 6 cut(s) 484, 500, 1066, 1267, 1515, 2109
BseGI GGATG 7 cut(s) 370, 402, 415, 903, 1595, 1754, 2019
BseJI GATNNNNATC 1 cut(s) 96
BseLI CCNNNNNNNGG 3 cut(s) 253, 1291, 1827
BseMI GCAATG 1 cut(s) 1770
BseNI ACTGG 2 cut(s) 1782, 2136
BseRI GAGGAG 2 cut(s) 269, 312
BseSI GKGCMC 3 cut(s) 1011, 1935, 2117
BseXI GCAGC 5 cut(s) 697, 700, 1693, 1696, 2113
BsgI GTGCAG 3 cut(s) 1023, 1562, 2112
BshFI GGCC 3 cut(s) 637, 1907, 2115
BsiSI CCGG 3 cut(s) 876, 1309, 1493
BslFI GGGAC 1 cut(s) 1509
BslI CCNNNNNNNGG 3 cut(s) 253, 1291, 1827
BsmAI GTCTC 5 cut(s) 345, 444, 1550, 1585, 1783
BsmFI GGGAC 1 cut(s) 1509
BsmI GAATGC 2 cut(s) 1329, 1467
BsnI GGCC 3 cut(s) 637, 1907, 2115
Bso31I GGTCTC 2 cut(s) 444, 1585
Bsp120I GGGCCC 1 cut(s) 2113
Bsp1286I GDGCHC 4 cut(s) 905, 1011, 1935, 2117
Bsp1407I TGTACA 1 cut(s) 1045
Bsp143I GATC 9 cut(s) 367, 1294, 1551, 1587, 1618, 1630, 1846, 1890, 1914
Bsp1720I GCTNAGC 2 cut(s) 15, 924
Bsp19I CCATGG 1 cut(s) 1066
BspANI GGCC 3 cut(s) 637, 1907, 2115
BspLI GGNNCC 5 cut(s) 615, 636, 1339, 1632, 2115
BspPI GGATC 5 cut(s) 1302, 1625, 1638, 1841, 1885
BspTI CTTAAG 2 cut(s) 278, 1475
BspTNI GGTCTC 2 cut(s) 444, 1585
BsrDI GCAATG 1 cut(s) 1770
BsrFI RCCGGY 1 cut(s) 1308
BsrGI TGTACA 1 cut(s) 1045
BsrI ACTGG 2 cut(s) 1782, 2136
BssAI RCCGGY 1 cut(s) 1308
BssECI CCNNGG 6 cut(s) 484, 500, 1066, 1267, 1515, 2109
BssMI GATC 9 cut(s) 367, 1294, 1551, 1587, 1618, 1630, 1846, 1890, 1914
BssSI CACGAG 1 cut(s) 1533
BssT1I CCWWGG 5 cut(s) 484, 500, 1066, 1515, 2109
Bst2BI CACGAG 1 cut(s) 1533
Bst2UI CCWGG 3 cut(s) 1268, 1822, 2024
Bst4CI ACNGT 1 cut(s) 1561
Bst6I CTCTTC 3 cut(s) 1677, 1995, 2124
BstAFI CTTAAG 2 cut(s) 278, 1475
BstAPI GCANNNNNTGC 1 cut(s) 1462
BstAUI TGTACA 1 cut(s) 1045
BstBAI YACGTR 1 cut(s) 1012
BstC8I GCNNGC 1 cut(s) 324
BstDSI CCRYGG 1 cut(s) 1066
BstEII GGTNACC 1 cut(s) 1480
BstENI CCTNNNNNAGG 1 cut(s) 1289
BstF5I GGATG 7 cut(s) 370, 402, 415, 903, 1595, 1754, 2019
BstHHI GCGC 1 cut(s) 2166
BstKTI GATC 9 cut(s) 370, 1297, 1554, 1590, 1621, 1633, 1849, 1893, 1917
BstMAI GTCTC 5 cut(s) 345, 444, 1550, 1585, 1783
BstMBI GATC 9 cut(s) 367, 1294, 1551, 1587, 1618, 1630, 1846, 1890, 1914
BstMWI GCNNNNNNNGC 3 cut(s) 1462, 1668, 2178
BstNI CCWGG 3 cut(s) 1268, 1822, 2024
BstNSI RCATGY 2 cut(s) 1444, 1781
BstPAI GACNNNNGTC 1 cut(s) 1262
BstPI GGTNACC 1 cut(s) 1480
BstSCI CCNGG 4 cut(s) 1266, 1492, 1820, 2022
BstSLI GKGCMC 3 cut(s) 1011, 1935, 2117
BstV1I GCAGC 5 cut(s) 697, 700, 1693, 1696, 2113
BstV2I GAAGAC 1 cut(s) 1083
BstX2I RGATCY 3 cut(s) 1630, 1846, 1890
BstXI CCANNNNNNTGG 1 cut(s) 140
BstYI RGATCY 3 cut(s) 1630, 1846, 1890
BsuI GTATCC 1 cut(s) 1265
BsuRI GGCC 3 cut(s) 637, 1907, 2115
BtgI CCRYGG 1 cut(s) 1066
BtsCI GGATG 7 cut(s) 370, 402, 415, 903, 1595, 1754, 2019
BtsI GCAGTG 1 cut(s) 1235
BtsIMutI CAGTG 6 cut(s) 966, 1235, 1677, 1789, 1809, 2129
Cac8I GCNNGC 1 cut(s) 324
CaiI CAGNNNCTG 1 cut(s) 112
CfoI GCGC 1 cut(s) 2166
Cfr10I RCCGGY 1 cut(s) 1308
Cfr13I GGNCC 7 cut(s) 635, 710, 1264, 1906, 2113, 2114, 2200
CseI GACGC 1 cut(s) 1730
Csp6I GTAC 4 cut(s) 1046, 1472, 1652, 1940
CviQI GTAC 4 cut(s) 1046, 1472, 1652, 1940
DpnI GATC 9 cut(s) 369, 1296, 1553, 1589, 1620, 1632, 1848, 1892, 1916
DpnII GATC 9 cut(s) 367, 1294, 1551, 1587, 1618, 1630, 1846, 1890, 1914
DraIII CACNNNGTG 1 cut(s) 136
Eam1104I CTCTTC 3 cut(s) 1677, 1995, 2124
EarI CTCTTC 3 cut(s) 1677, 1995, 2124
Eco130I CCWWGG 5 cut(s) 484, 500, 1066, 1515, 2109
Eco24I GRGCYC 2 cut(s) 905, 2117
Eco31I GGTCTC 2 cut(s) 444, 1585
Eco47I GGWCC 3 cut(s) 710, 1264, 2200
Eco57I CTGAAG 5 cut(s) 101, 1657, 1701, 1833, 1907
Eco91I GGTNACC 1 cut(s) 1480
EcoNI CCTNNNNNAGG 1 cut(s) 1289
EcoO109I RGGNCCY 3 cut(s) 2113, 2114, 2200
EcoO65I GGTNACC 1 cut(s) 1480
EcoRII CCWGG 3 cut(s) 1266, 1820, 2022
EcoT14I CCWWGG 5 cut(s) 484, 500, 1066, 1515, 2109
EcoT22I ATGCAT 1 cut(s) 1772
EcoT38I GRGCYC 2 cut(s) 905, 2117
ErhI CCWWGG 5 cut(s) 484, 500, 1066, 1515, 2109
FalI AAGNNNNNCTT 2 cut(s) 1919, 1951
FaqI GGGAC 1 cut(s) 1509
FauNDI CATATG 2 cut(s) 681, 808
FbaI TGATCA 1 cut(s) 1587
Fnu4HI GCNGC 5 cut(s) 686, 689, 1707, 1710, 2127
FokI GGATG 7 cut(s) 377, 409, 422, 910, 1582, 1761, 2006
FriOI GRGCYC 2 cut(s) 905, 2117
Fsp4HI GCNGC 5 cut(s) 686, 689, 1707, 1710, 2127
GlaI GCGC 1 cut(s) 2165
GluI GCNGC 5 cut(s) 686, 689, 1707, 1710, 2127
HaeIII GGCC 3 cut(s) 637, 1907, 2115
HapII CCGG 3 cut(s) 876, 1309, 1493
HgaI GACGC 1 cut(s) 1730
HhaI GCGC 1 cut(s) 2166
Hin6I GCGC 1 cut(s) 2164
HinP1I GCGC 1 cut(s) 2164
HindIII AAGCTT 1 cut(s) 561
HpaII CCGG 3 cut(s) 876, 1309, 1493
HphI GGTGA 5 cut(s) 122, 138, 665, 1040, 1492
Hpy166II GTNNAC 3 cut(s) 146, 937, 947
Hpy188I TCNGA 5 cut(s) 175, 220, 553, 897, 1212
Hpy188III TCNNGA 7 cut(s) 1158, 1346, 1388, 1555, 1616, 1796, 2206
Hpy8I GTNNAC 3 cut(s) 146, 937, 947
HpyAV CCTTC 5 cut(s) 195, 205, 1136, 1352, 1921
HpyCH4III ACNGT 1 cut(s) 1561
HpyCH4IV ACGT 1 cut(s) 1011
HpyF10VI GCNNNNNNNGC 3 cut(s) 1462, 1668, 2178
HpySE526I ACGT 1 cut(s) 1011
HspAI GCGC 1 cut(s) 2164
Ksp22I TGATCA 1 cut(s) 1587
Kzo9I GATC 9 cut(s) 367, 1294, 1551, 1587, 1618, 1630, 1846, 1890, 1914
LmnI GCTCC 1 cut(s) 613
Lsp1109I GCAGC 5 cut(s) 697, 700, 1693, 1696, 2113
LweI GCATC 3 cut(s) 1116, 1693, 1739
MaeII ACGT 1 cut(s) 1011
MaeIII GTNAC 6 cut(s) 737, 1072, 1233, 1480, 1807, 2104
MalI GATC 9 cut(s) 369, 1296, 1553, 1589, 1620, 1632, 1848, 1892, 1916
MboI GATC 9 cut(s) 367, 1294, 1551, 1587, 1618, 1630, 1846, 1890, 1914
MboII GAAGA 7 cut(s) 58, 1083, 1120, 1694, 1900, 2012, 2111
MflI RGATCY 3 cut(s) 1630, 1846, 1890
MhlI GDGCHC 4 cut(s) 905, 1011, 1935, 2117
MluCI AATT 4 cut(s) 836, 1414, 1466, 2027
MlyI GAGTC 3 cut(s) 308, 347, 1217
MmeI TCCRAC 1 cut(s) 291
Mph1103I ATGCAT 1 cut(s) 1772
MroXI GAANNNNTTC 2 cut(s) 564, 865
MseI TTAA 4 cut(s) 279, 600, 1476, 1626
MslI CAYNNNNRTG 9 cut(s) 132, 138, 348, 420, 582, 853, 1124, 1535, 1782
MspA1I CMGCKG 2 cut(s) 928, 2056
MspCI CTTAAG 2 cut(s) 278, 1475
MspI CCGG 3 cut(s) 876, 1309, 1493
MspR9I CCNGG 4 cut(s) 1268, 1494, 1822, 2024
Mva1269I GAATGC 2 cut(s) 1329, 1467
MvaI CCWGG 3 cut(s) 1268, 1822, 2024
MwoI GCNNNNNNNGC 3 cut(s) 1462, 1668, 2178
NciI CCSGG 1 cut(s) 1494
NcoI CCATGG 1 cut(s) 1066
NdeI CATATG 2 cut(s) 681, 808
NdeII GATC 9 cut(s) 367, 1294, 1551, 1587, 1618, 1630, 1846, 1890, 1914
NlaIV GGNNCC 5 cut(s) 615, 636, 1339, 1632, 2115
NmeAIII GCCGAG 1 cut(s) 2033
NmuCI GTSAC 4 cut(s) 1072, 1233, 1480, 1807
NsiI ATGCAT 1 cut(s) 1772
NspI RCATGY 2 cut(s) 1444, 1781
OliI CACNNNNGTG 1 cut(s) 1535
PciI ACATGT 1 cut(s) 1440
PctI GAATGC 2 cut(s) 1329, 1467
PdmI GAANNNNTTC 2 cut(s) 564, 865
PfeI GAWTC 7 cut(s) 72, 92, 656, 802, 866, 1172, 1716
PfoI TCCNGGA 2 cut(s) 1820, 2022
PkrI GCNGC 5 cut(s) 687, 690, 1708, 1711, 2128
PleI GAGTC 3 cut(s) 308, 346, 1217
PpsI GAGTC 3 cut(s) 308, 346, 1217
Ppu21I YACGTR 1 cut(s) 1012
PpuMI RGGWCCY 1 cut(s) 2200
PscI ACATGT 1 cut(s) 1440
PshAI GACNNNNGTC 1 cut(s) 1262
PsiI TTATAA 1 cut(s) 348
Psp5II RGGWCCY 1 cut(s) 2200
Psp6I CCWGG 3 cut(s) 1266, 1820, 2022
PspEI GGTNACC 1 cut(s) 1480
PspGI CCWGG 3 cut(s) 1266, 1820, 2022
PspN4I GGNNCC 5 cut(s) 615, 636, 1339, 1632, 2115
PspOMI GGGCCC 1 cut(s) 2113
PspPI GGNCC 7 cut(s) 635, 710, 1264, 1906, 2113, 2114, 2200
PspPPI RGGWCCY 1 cut(s) 2200
PstNI CAGNNNCTG 1 cut(s) 112
PsuI RGATCY 3 cut(s) 1630, 1846, 1890
PvuII CAGCTG 2 cut(s) 928, 2056
RsaI GTAC 4 cut(s) 1047, 1473, 1653, 1941
RsaNI GTAC 4 cut(s) 1046, 1472, 1652, 1940
RseI CAYNNNNRTG 9 cut(s) 132, 138, 348, 420, 582, 853, 1124, 1535, 1782
SaqAI TTAA 4 cut(s) 279, 600, 1476, 1626
SatI GCNGC 5 cut(s) 686, 689, 1707, 1710, 2127
Sau3AI GATC 9 cut(s) 367, 1294, 1551, 1587, 1618, 1630, 1846, 1890, 1914
Sau96I GGNCC 7 cut(s) 635, 710, 1264, 1906, 2113, 2114, 2200
SchI GAGTC 3 cut(s) 308, 347, 1217
ScrFI CCNGG 4 cut(s) 1268, 1494, 1822, 2024
SduI GDGCHC 4 cut(s) 905, 1011, 1935, 2117
SfaNI GCATC 3 cut(s) 1116, 1693, 1739
SinI GGWCC 3 cut(s) 710, 1264, 2200
SmiMI CAYNNNNRTG 9 cut(s) 132, 138, 348, 420, 582, 853, 1124, 1535, 1782
SmlI CTYRAG 3 cut(s) 278, 1475, 1988
SmoI CTYRAG 3 cut(s) 278, 1475, 1988
Sse9I AATT 4 cut(s) 836, 1414, 1466, 2027
SspI AATATT 2 cut(s) 496, 1362
StyD4I CCNGG 4 cut(s) 1266, 1492, 1820, 2022
StyI CCWWGG 5 cut(s) 484, 500, 1066, 1515, 2109
TaaI ACNGT 1 cut(s) 1561
TaiI ACGT 1 cut(s) 1014
TaqI TCGA 3 cut(s) 1329, 1554, 1795
TasI AATT 4 cut(s) 836, 1414, 1466, 2027
TatI WGTACW 3 cut(s) 1045, 1471, 1939
TfiI GAWTC 7 cut(s) 72, 92, 656, 802, 866, 1172, 1716
Tru1I TTAA 4 cut(s) 279, 600, 1476, 1626
Tru9I TTAA 4 cut(s) 279, 600, 1476, 1626
TscAI CASTG 6 cut(s) 966, 1242, 1684, 1789, 1816, 2136
TseFI GTSAC 4 cut(s) 1072, 1233, 1480, 1807
TseI GCWGC 5 cut(s) 685, 688, 1706, 1709, 2126
Tsp45I GTSAC 4 cut(s) 1072, 1233, 1480, 1807
TspGWI ACGGA 1 cut(s) 1839
TspRI CASTG 6 cut(s) 966, 1242, 1684, 1789, 1816, 2136
Vha464I CTTAAG 2 cut(s) 278, 1475
VpaK11BI GGWCC 3 cut(s) 710, 1264, 2200
XagI CCTNNNNNAGG 1 cut(s) 1289
XapI RAATTY 1 cut(s) 836
XbaI TCTAGA 1 cut(s) 1157
XceI RCATGY 2 cut(s) 1444, 1781
XcmI CCANNNNNNNNNTGG 1 cut(s) 1850
XmaJI CCTAGG 1 cut(s) 484
XmnI GAANNNNTTC 2 cut(s) 564, 865
Zsp2I ATGCAT 1 cut(s) 1772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.