RchiOBHm_Chr7g0239301

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0012349
Physical Location & Seq
Reverse (-)
64780152 .. 64784650
4499 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21444

Sequence Viewer

Length: 2235 bp
ATGTTGAGAATAGCTGAGCAAGACCAGAGGGTTTTGATGAACATGAAGAATGATAACAACAACAACAACAACAACAACCCAAGAATCCCTCCACCTTATTGTGATTCCAATCTTTTTCAGCTTCTGCTTCAGTCTAACAACCATCACCATGTGAGTGGTTCACCTATTAGCAGAGCTTCTCTGCAGTCTGAGTTTTCTCCTTCGAGTTCTTTCTCAAATGGGTTTTATTCCTCTGAGGATAGCTCTCCTTATTCTCCAACCCCATTTGAGGAGGCAAAGTATCAAACACCTCTTAAGCATTACATGAATGGAGGAGGGTTGTGGTTGGACTCCAAGCCAGCACCACACCATGAGTCTCATTATAATGAGATTTTGGGGGATGATCTGGGGTTGGCTGAGAGTTTTTACAGAATGAATATTGGGGATGGACATGAGGGTGGTGGTAAAAGGAGGGGGTTTGAGAGAGACCCAGATGGGTTTGGGCTTGGTGGTGGTTTCTTAGGGGGTAATTTTCCTTGGAATGTTGAGAACTATGGCTTGTTTGAAACTTCTAGGAACAATATGTCTGATGTTGAAGCTTTTCAGTCCTCTCATTTTGGTGTTAGAGGTGGTTTTAATGGTTATGTGGAGCCTGAGTTTCTTGGGTTGGGGCCAGAATATGTTGTTGGTGATTCAATGGGGGCTGTTCTAAATCATATGCAGCAGCCTAGTGCTTTGTGTTGTGGTCCTAGTGGTGCTAAGAACCAAATGGGTTACATGGTAGAGCAAAGAAAGGAACTGAGAGATGATTGGTATTACAGAAATGTTCAAGTAGAGAACCCATATGTTGCCAGACCTTACCTTGATGATGAATTTGTTTATTCACAGCTATGTGGAACAGATTCTAGTGCCGGAAAGGGTCTTATGTATCCTATGAGCTCTCCTCAGTTCCCCTCAGTTGCTCAGCTGTGTGTGGACAATGGTGCACATAATCATCCAGTGATAAAACAAAGGACTAGAGGAAATCCGACTAGGAAATGTGCAGGGCACTTAGAGGGTTTTAGAGGTGAGGATAGTCTTGATGTACAAGGAAAACATTTGAACCATGGTGTCACTAGGGGTAGCAAGTCTTCAAAGGCTAACAAGAAGAACTCTTGCACTAAGATGCCAATGAGGACAGAAGGAGAGCAAAGCTCTAGATTAGATTTGGATTCATATAATGGAGGAAGTAGTGGAAATGAATGTAGTCAGAGCAGTAATGACTCAATGAAGTCGCTGCTTACTTTGGGTTCATTGACTGAGGTCCAGGGATACATATACTTCCTAGCAAAGGATCAGCATGGTTGCCGGTTCTTACAGAGAATGCTCGATGAAGGAACCCGTCAAGATGTGCAATTAATATTTGATAGGATAATCAATCATGTTCTTGAACTTATGACGGACCCGTTTGGCAATTACCTAATGCAGAAGTTCTTGGACATGTGTAATGAACAGCAAAGAATGCAGTTTGTACTTAAGGTGACCAAAGAACCGGGACAGCTTGCGAGAGTTTCCTTGGACACACATGGCACTCGTGTGGTGCAGAAGTTGATCGAGACTGTGGAAAATGGGAAGCAGGTCTCTTTGATCATATCATCCCTTGAATGTGGTTTTCTTGATCTTATTAAGGATCCAAATGGCAATCATGTGGTACAGCGTTGCTTGGACTGCTTCAGCACTGAAGAGAACAGGTTTATATTTGATGCTGCTGCAAGATTCTGTGTTGAGATTGCAACTCAGCGTCATGGATGCTGTGTGCTACAAAAATGCATTGCACATGCCAGTGGGAGACATCGAGATAAGCTGGTCACTGAAGTTTCTAGGAATGGGCTTCTCCTTTCCCAAGATCCGTATGGAAACTATGTTGTTCAGTATGTCATAGAGCTGAAAATCCCATCAGTCATGGCCAGATTGATCTCTCAGTTGAAGGGACACTTTGTACTCCTCTCCATGCAGAAATGCAGTAGTCATGTTGTTGAAAAATGCCTCAAGCACTATGAAGAGAGCCGAGCAAAAGTCATCCAGGAATTGCTCTCAGTTTCTCACTTTGAACAGCTGTTGCAAGACCCTTATGCTAACTATGTCATTCAATCTGCTCTTTCAGTTACCAAGGGCCCTCTTCACTCTGCACTGGTTGAAGCTGTCAAGCCTCATACAATCTTGCGCCACAGCCCATACTGCAAGAGGATTTTCACAAAGGACCTCCTGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

744

Amino Acids

83.44

Weight (kDa)

6.65

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 432 - 465 4.3e-11 Pumilio-family RNA binding repeat
PUF PF00806 467 - 499 5.8e-09 Pumilio-family RNA binding repeat
PUF PF00806 544 - 574 3.6e-08 Pumilio-family RNA binding repeat
PUF PF00806 580 - 612 7.8e-13 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012349)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22240 AT1G78160 AT1G78160
fragaria_vesca FvH4_5g37620
malus_domestica MD08G1181900.v1.1 MD15G1367200.v1.1
prunus_persica Prupe.1G515100_v2.0.a1
pyrus_communis pycom08g15510 pycom15g32920
rosa_chinensis RchiOBHm_Chr7g0239301
rosa_laevigata RLG00000000765
rosa_multiflora Rmu_sc0007840.1_g000004
rosa_roxburghii Rroxscaffold_3G00221040
rosa_rugosa Rorug07G0327100
rosa_samantha Rh7AG480500 Rh7BG453200 Rh7CG498400 Rh7DG466500
rosa_wichuraiana Rw7G040250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 363
Acc36I ACCTGC 1 cut(s) 1585
AclWI GGATC 4 cut(s) 1320, 1643, 1656, 1859
AcoI YGGCCR 1 cut(s) 1923
AcsI RAATTY 1 cut(s) 851
AcuI CTGAAG 4 cut(s) 113, 1675, 1719, 1851
AdeI CACNNNGTG 1 cut(s) 151
AfaI GTAC 4 cut(s) 1065, 1491, 1671, 1959
AfiI CCNNNNNNNGG 2 cut(s) 268, 1309
AflII CTTAAG 2 cut(s) 293, 1493
AflIII ACRYGT 1 cut(s) 1458
AjnI CCWGG 2 cut(s) 1284, 2040
AjuI GAANNNNNNNTTGG 2 cut(s) 648, 680
AleI CACNNNNGTG 1 cut(s) 1553
Alw21I GWGCWC 2 cut(s) 920, 967
Alw26I GTCTC 5 cut(s) 360, 459, 1568, 1603, 1801
Alw44I GTGCAC 1 cut(s) 963
AlwI GGATC 4 cut(s) 1320, 1643, 1656, 1859
AlwNI CAGNNNCTG 1 cut(s) 124
AoxI GGCC 3 cut(s) 650, 1923, 2131
ApaI GGGCCC 1 cut(s) 2135
ApaLI GTGCAC 1 cut(s) 963
ApeKI GCWGC 5 cut(s) 700, 703, 1255, 1724, 1727
ApoI RAATTY 1 cut(s) 851
AseI ATTAAT 1 cut(s) 1376
Asp700I GAANNNNTTC 2 cut(s) 579, 880
AspLEI GCGC 1 cut(s) 2184
AspS9I GGNCC 7 cut(s) 650, 725, 1282, 1420, 2131, 2132, 2218
AsuC2I CCSGG 1 cut(s) 1512
AsuHPI GGTGA 5 cut(s) 137, 153, 680, 1058, 1510
AvaII GGWCC 4 cut(s) 725, 1282, 1420, 2218
BaeGI GKGCMC 3 cut(s) 967, 1029, 2135
BalI TGGCCA 1 cut(s) 1925
BamHI GGATCC 1 cut(s) 1648
BanII GRGCYC 2 cut(s) 920, 2135
BarI GAAGNNNNNNTAC 2 cut(s) 1093, 1125
BauI CACGAG 1 cut(s) 1551
BbsI GAAGAC 1 cut(s) 1101
Bbv12I GWGCWC 2 cut(s) 920, 967
BbvI GCAGC 5 cut(s) 712, 715, 1242, 1711, 1714
BccI CCATC 4 cut(s) 150, 419, 467, 1921
BciT130I CCWGG 2 cut(s) 1286, 2042
BciVI GTATCC 2 cut(s) 918, 1283
BclI TGATCA 1 cut(s) 1605
BcnI CCSGG 1 cut(s) 1512
BcoDI GTCTC 5 cut(s) 360, 459, 1568, 1603, 1801
BfmI CTRYAG 1 cut(s) 182
BfrI CTTAAG 2 cut(s) 293, 1493
BfuAI ACCTGC 1 cut(s) 1585
BfuI GTATCC 2 cut(s) 918, 1283
BisI GCNGC 5 cut(s) 701, 704, 1256, 1725, 1728
BlpI GCTNAGC 2 cut(s) 15, 942
BlsI GCNGC 5 cut(s) 702, 705, 1257, 1726, 1729
Bme1390I CCNGG 3 cut(s) 1286, 1512, 2042
Bme18I GGWCC 4 cut(s) 725, 1282, 1420, 2218
BmgT120I GGNCC 7 cut(s) 650, 725, 1282, 1420, 2131, 2132, 2218
BmiI GGNNCC 6 cut(s) 630, 651, 1357, 1422, 1650, 2133
BmrFI CCNGG 3 cut(s) 1286, 1512, 2042
BmsI GCATC 3 cut(s) 1134, 1711, 1757
BoxI GACNNNNGTC 1 cut(s) 1280
BpiI GAAGAC 1 cut(s) 1101
BplI GAGNNNNNCTC 6 cut(s) 227, 259, 907, 939, 1157, 1189
Bpu1102I GCTNAGC 2 cut(s) 15, 942
BpuEI CTTGAG 1 cut(s) 1991
BpuMI CCSGG 1 cut(s) 1512
BsaBI GATNNNNATC 1 cut(s) 108
BsaI GGTCTC 2 cut(s) 459, 1603
BsaJI CCNNGG 5 cut(s) 515, 1084, 1285, 1533, 2127
BsaXI ACNNNNNCTCC 4 cut(s) 263, 293, 620, 650
Bsc4I CCNNNNNNNGG 2 cut(s) 268, 1309
Bse118I RCCGGY 1 cut(s) 1326
Bse1I ACTGG 3 cut(s) 977, 1800, 2154
Bse3DI GCAATG 1 cut(s) 1788
Bse8I GATNNNNATC 1 cut(s) 108
BseBI CCWGG 2 cut(s) 1286, 2042
BseDI CCNNGG 5 cut(s) 515, 1084, 1285, 1533, 2127
BseGI GGATG 6 cut(s) 385, 430, 973, 1613, 1772, 2037
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 2 cut(s) 268, 1309
BseMI GCAATG 1 cut(s) 1788
BseNI ACTGG 3 cut(s) 977, 1800, 2154
BseRI GAGGAG 4 cut(s) 284, 327, 912, 1952
BseSI GKGCMC 3 cut(s) 967, 1029, 2135
BseXI GCAGC 5 cut(s) 712, 715, 1242, 1711, 1714
BsgI GTGCAG 3 cut(s) 1041, 1580, 2130
BshFI GGCC 3 cut(s) 652, 1925, 2133
BsiHKAI GWGCWC 2 cut(s) 920, 967
BsiSI CCGG 3 cut(s) 891, 1327, 1511
BslFI GGGAC 2 cut(s) 1527, 1962
BslI CCNNNNNNNGG 2 cut(s) 268, 1309
BsmAI GTCTC 5 cut(s) 360, 459, 1568, 1603, 1801
BsmFI GGGAC 2 cut(s) 1527, 1962
BsmI GAATGC 2 cut(s) 1347, 1485
BsnI GGCC 3 cut(s) 652, 1925, 2133
Bso31I GGTCTC 2 cut(s) 459, 1603
Bsp120I GGGCCC 1 cut(s) 2131
Bsp1286I GDGCHC 4 cut(s) 920, 967, 1029, 2135
Bsp1407I TGTACA 1 cut(s) 1063
Bsp143I GATC 8 cut(s) 382, 1312, 1569, 1605, 1636, 1648, 1864, 1932
Bsp1720I GCTNAGC 2 cut(s) 15, 942
Bsp19I CCATGG 1 cut(s) 1084
BspANI GGCC 3 cut(s) 652, 1925, 2133
BspLI GGNNCC 6 cut(s) 630, 651, 1357, 1422, 1650, 2133
BspMAI CTGCAG 1 cut(s) 186
BspMI ACCTGC 1 cut(s) 1585
BspPI GGATC 4 cut(s) 1320, 1643, 1656, 1859
BspTI CTTAAG 2 cut(s) 293, 1493
BspTNI GGTCTC 2 cut(s) 459, 1603
BsrDI GCAATG 1 cut(s) 1788
BsrFI RCCGGY 1 cut(s) 1326
BsrGI TGTACA 1 cut(s) 1063
BsrI ACTGG 3 cut(s) 977, 1800, 2154
BssAI RCCGGY 1 cut(s) 1326
BssECI CCNNGG 5 cut(s) 515, 1084, 1285, 1533, 2127
BssMI GATC 8 cut(s) 382, 1312, 1569, 1605, 1636, 1648, 1864, 1932
BssSI CACGAG 1 cut(s) 1551
BssT1I CCWWGG 4 cut(s) 515, 1084, 1533, 2127
Bst2BI CACGAG 1 cut(s) 1551
Bst2UI CCWGG 2 cut(s) 1286, 2042
Bst4CI ACNGT 1 cut(s) 1579
Bst6I CTCTTC 3 cut(s) 1695, 2013, 2142
BstAFI CTTAAG 2 cut(s) 293, 1493
BstAPI GCANNNNNTGC 1 cut(s) 1480
BstAUI TGTACA 1 cut(s) 1063
BstC8I GCNNGC 2 cut(s) 339, 1521
BstDSI CCRYGG 1 cut(s) 1084
BstEII GGTNACC 1 cut(s) 1498
BstENI CCTNNNNNAGG 1 cut(s) 1307
BstF5I GGATG 6 cut(s) 385, 430, 973, 1613, 1772, 2037
BstHHI GCGC 1 cut(s) 2184
BstKTI GATC 8 cut(s) 385, 1315, 1572, 1608, 1639, 1651, 1867, 1935
BstMAI GTCTC 5 cut(s) 360, 459, 1568, 1603, 1801
BstMBI GATC 8 cut(s) 382, 1312, 1569, 1605, 1636, 1648, 1864, 1932
BstMWI GCNNNNNNNGC 3 cut(s) 1480, 1686, 2196
BstNI CCWGG 2 cut(s) 1286, 2042
BstNSI RCATGY 2 cut(s) 1462, 1799
BstPAI GACNNNNGTC 1 cut(s) 1280
BstPI GGTNACC 1 cut(s) 1498
BstSCI CCNGG 3 cut(s) 1284, 1510, 2040
BstSFI CTRYAG 1 cut(s) 182
BstSLI GKGCMC 3 cut(s) 967, 1029, 2135
BstV1I GCAGC 5 cut(s) 712, 715, 1242, 1711, 1714
BstV2I GAAGAC 1 cut(s) 1101
BstX2I RGATCY 2 cut(s) 1648, 1864
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 2 cut(s) 1648, 1864
BsuI GTATCC 2 cut(s) 918, 1283
BsuRI GGCC 3 cut(s) 652, 1925, 2133
BtgI CCRYGG 1 cut(s) 1084
BtsCI GGATG 6 cut(s) 385, 430, 973, 1613, 1772, 2037
BtsIMutI CAGTG 5 cut(s) 984, 1695, 1807, 1827, 2147
BveI ACCTGC 1 cut(s) 1585
Cac8I GCNNGC 2 cut(s) 339, 1521
CaiI CAGNNNCTG 1 cut(s) 124
CfoI GCGC 1 cut(s) 2184
Cfr10I RCCGGY 1 cut(s) 1326
Cfr13I GGNCC 7 cut(s) 650, 725, 1282, 1420, 2131, 2132, 2218
CseI GACGC 1 cut(s) 1748
Csp6I GTAC 4 cut(s) 1064, 1490, 1670, 1958
CviQI GTAC 4 cut(s) 1064, 1490, 1670, 1958
DpnI GATC 8 cut(s) 384, 1314, 1571, 1607, 1638, 1650, 1866, 1934
DpnII GATC 8 cut(s) 382, 1312, 1569, 1605, 1636, 1648, 1864, 1932
DraIII CACNNNGTG 1 cut(s) 151
EaeI YGGCCR 1 cut(s) 1923
Eam1104I CTCTTC 3 cut(s) 1695, 2013, 2142
EarI CTCTTC 3 cut(s) 1695, 2013, 2142
Ecl136II GAGCTC 1 cut(s) 918
Eco130I CCWWGG 4 cut(s) 515, 1084, 1533, 2127
Eco24I GRGCYC 2 cut(s) 920, 2135
Eco31I GGTCTC 2 cut(s) 459, 1603
Eco47I GGWCC 4 cut(s) 725, 1282, 1420, 2218
Eco53kI GAGCTC 1 cut(s) 918
Eco57I CTGAAG 4 cut(s) 113, 1675, 1719, 1851
Eco91I GGTNACC 1 cut(s) 1498
EcoICRI GAGCTC 1 cut(s) 918
EcoNI CCTNNNNNAGG 1 cut(s) 1307
EcoO109I RGGNCCY 3 cut(s) 2131, 2132, 2218
EcoO65I GGTNACC 1 cut(s) 1498
EcoRII CCWGG 2 cut(s) 1284, 2040
EcoT14I CCWWGG 4 cut(s) 515, 1084, 1533, 2127
EcoT22I ATGCAT 1 cut(s) 1790
EcoT38I GRGCYC 2 cut(s) 920, 2135
ErhI CCWWGG 4 cut(s) 515, 1084, 1533, 2127
FalI AAGNNNNNCTT 2 cut(s) 1937, 1969
FaqI GGGAC 2 cut(s) 1527, 1962
FauNDI CATATG 2 cut(s) 696, 823
FbaI TGATCA 1 cut(s) 1605
Fnu4HI GCNGC 5 cut(s) 701, 704, 1256, 1725, 1728
FokI GGATG 6 cut(s) 392, 437, 960, 1600, 1779, 2024
FriOI GRGCYC 2 cut(s) 920, 2135
Fsp4HI GCNGC 5 cut(s) 701, 704, 1256, 1725, 1728
GlaI GCGC 1 cut(s) 2183
GluI GCNGC 5 cut(s) 701, 704, 1256, 1725, 1728
HaeIII GGCC 3 cut(s) 652, 1925, 2133
HapII CCGG 3 cut(s) 891, 1327, 1511
HgaI GACGC 1 cut(s) 1748
HhaI GCGC 1 cut(s) 2184
Hin6I GCGC 1 cut(s) 2182
HinP1I GCGC 1 cut(s) 2182
HindIII AAGCTT 1 cut(s) 576
HinfI GANTC 9 cut(s) 84, 104, 329, 353, 671, 881, 1190, 1241, 1734
HpaII CCGG 3 cut(s) 891, 1327, 1511
HphI GGTGA 5 cut(s) 137, 153, 680, 1058, 1510
Hpy166II GTNNAC 3 cut(s) 161, 955, 965
Hpy188I TCNGA 5 cut(s) 190, 235, 568, 1008, 1230
Hpy188III TCNNGA 8 cut(s) 1058, 1176, 1364, 1406, 1573, 1634, 1814, 2224
Hpy8I GTNNAC 3 cut(s) 161, 955, 965
HpyAV CCTTC 4 cut(s) 210, 1154, 1346, 1939
HpyCH4III ACNGT 1 cut(s) 1579
HpyF10VI GCNNNNNNNGC 3 cut(s) 1480, 1686, 2196
HspAI GCGC 1 cut(s) 2182
Ksp22I TGATCA 1 cut(s) 1605
Kzo9I GATC 8 cut(s) 382, 1312, 1569, 1605, 1636, 1648, 1864, 1932
LmnI GCTCC 1 cut(s) 628
Lsp1109I GCAGC 5 cut(s) 712, 715, 1242, 1711, 1714
LweI GCATC 3 cut(s) 1134, 1711, 1757
MaeIII GTNAC 5 cut(s) 752, 1090, 1498, 1825, 2122
MalI GATC 8 cut(s) 384, 1314, 1571, 1607, 1638, 1650, 1866, 1934
MboI GATC 8 cut(s) 382, 1312, 1569, 1605, 1636, 1648, 1864, 1932
MboII GAAGA 6 cut(s) 58, 1101, 1138, 1712, 2030, 2129
MflI RGATCY 2 cut(s) 1648, 1864
MhlI GDGCHC 4 cut(s) 920, 967, 1029, 2135
MlsI TGGCCA 1 cut(s) 1925
MluCI AATT 5 cut(s) 508, 851, 1373, 1432, 2045
MluNI TGGCCA 1 cut(s) 1925
MlyI GAGTC 3 cut(s) 323, 362, 1235
MmeI TCCRAC 3 cut(s) 281, 306, 1031
Mox20I TGGCCA 1 cut(s) 1925
Mph1103I ATGCAT 1 cut(s) 1790
MroXI GAANNNNTTC 2 cut(s) 579, 880
MscI TGGCCA 1 cut(s) 1925
MseI TTAA 5 cut(s) 294, 615, 1376, 1494, 1644
MslI CAYNNNNRTG 9 cut(s) 147, 153, 363, 435, 597, 868, 1142, 1553, 1800
Msp20I TGGCCA 1 cut(s) 1925
MspA1I CMGCKG 2 cut(s) 946, 2074
MspCI CTTAAG 2 cut(s) 293, 1493
MspI CCGG 3 cut(s) 891, 1327, 1511
MspR9I CCNGG 3 cut(s) 1286, 1512, 2042
Mva1269I GAATGC 2 cut(s) 1347, 1485
MvaI CCWGG 2 cut(s) 1286, 2042
MwoI GCNNNNNNNGC 3 cut(s) 1480, 1686, 2196
NciI CCSGG 1 cut(s) 1512
NcoI CCATGG 1 cut(s) 1084
NdeI CATATG 2 cut(s) 696, 823
NdeII GATC 8 cut(s) 382, 1312, 1569, 1605, 1636, 1648, 1864, 1932
NlaIV GGNNCC 6 cut(s) 630, 651, 1357, 1422, 1650, 2133
NmeAIII GCCGAG 1 cut(s) 2051
NmuCI GTSAC 3 cut(s) 1090, 1498, 1825
NsiI ATGCAT 1 cut(s) 1790
NspI RCATGY 2 cut(s) 1462, 1799
OliI CACNNNNGTG 1 cut(s) 1553
PciI ACATGT 1 cut(s) 1458
PctI GAATGC 2 cut(s) 1347, 1485
PdmI GAANNNNTTC 2 cut(s) 579, 880
PfeI GAWTC 6 cut(s) 84, 104, 671, 881, 1190, 1734
PfoI TCCNGGA 1 cut(s) 2040
PkrI GCNGC 5 cut(s) 702, 705, 1257, 1726, 1729
PleI GAGTC 3 cut(s) 323, 361, 1235
PpsI GAGTC 3 cut(s) 323, 361, 1235
PpuMI RGGWCCY 1 cut(s) 2218
PscI ACATGT 1 cut(s) 1458
PshAI GACNNNNGTC 1 cut(s) 1280
PshBI ATTAAT 1 cut(s) 1376
PsiI TTATAA 1 cut(s) 363
Psp124BI GAGCTC 1 cut(s) 920
Psp5II RGGWCCY 1 cut(s) 2218
Psp6I CCWGG 2 cut(s) 1284, 2040
PspEI GGTNACC 1 cut(s) 1498
PspGI CCWGG 2 cut(s) 1284, 2040
PspN4I GGNNCC 6 cut(s) 630, 651, 1357, 1422, 1650, 2133
PspOMI GGGCCC 1 cut(s) 2131
PspPI GGNCC 7 cut(s) 650, 725, 1282, 1420, 2131, 2132, 2218
PspPPI RGGWCCY 1 cut(s) 2218
PstI CTGCAG 1 cut(s) 186
PstNI CAGNNNCTG 1 cut(s) 124
PsuI RGATCY 2 cut(s) 1648, 1864
PvuII CAGCTG 2 cut(s) 946, 2074
RsaI GTAC 4 cut(s) 1065, 1491, 1671, 1959
RsaNI GTAC 4 cut(s) 1064, 1490, 1670, 1958
RseI CAYNNNNRTG 9 cut(s) 147, 153, 363, 435, 597, 868, 1142, 1553, 1800
SacI GAGCTC 1 cut(s) 920
SaqAI TTAA 5 cut(s) 294, 615, 1376, 1494, 1644
SatI GCNGC 5 cut(s) 701, 704, 1256, 1725, 1728
Sau3AI GATC 8 cut(s) 382, 1312, 1569, 1605, 1636, 1648, 1864, 1932
Sau96I GGNCC 7 cut(s) 650, 725, 1282, 1420, 2131, 2132, 2218
SchI GAGTC 3 cut(s) 323, 362, 1235
ScrFI CCNGG 3 cut(s) 1286, 1512, 2042
SduI GDGCHC 4 cut(s) 920, 967, 1029, 2135
SfaNI GCATC 3 cut(s) 1134, 1711, 1757
SfcI CTRYAG 1 cut(s) 182
SinI GGWCC 4 cut(s) 725, 1282, 1420, 2218
SmiMI CAYNNNNRTG 9 cut(s) 147, 153, 363, 435, 597, 868, 1142, 1553, 1800
SmlI CTYRAG 3 cut(s) 293, 1493, 2006
SmoI CTYRAG 3 cut(s) 293, 1493, 2006
Sse9I AATT 5 cut(s) 508, 851, 1373, 1432, 2045
SspI AATATT 2 cut(s) 418, 1380
SstI GAGCTC 1 cut(s) 920
StyD4I CCNGG 3 cut(s) 1284, 1510, 2040
StyI CCWWGG 4 cut(s) 515, 1084, 1533, 2127
TaaI ACNGT 1 cut(s) 1579
TaqI TCGA 4 cut(s) 203, 1347, 1572, 1813
TasI AATT 5 cut(s) 508, 851, 1373, 1432, 2045
TatI WGTACW 3 cut(s) 1063, 1489, 1957
TfiI GAWTC 6 cut(s) 84, 104, 671, 881, 1190, 1734
Tru1I TTAA 5 cut(s) 294, 615, 1376, 1494, 1644
Tru9I TTAA 5 cut(s) 294, 615, 1376, 1494, 1644
TscAI CASTG 5 cut(s) 984, 1702, 1807, 1834, 2154
TseFI GTSAC 3 cut(s) 1090, 1498, 1825
TseI GCWGC 5 cut(s) 700, 703, 1255, 1724, 1727
Tsp45I GTSAC 3 cut(s) 1090, 1498, 1825
TspGWI ACGGA 2 cut(s) 1433, 1857
TspRI CASTG 5 cut(s) 984, 1702, 1807, 1834, 2154
Vha464I CTTAAG 2 cut(s) 293, 1493
VneI GTGCAC 1 cut(s) 963
VpaK11BI GGWCC 4 cut(s) 725, 1282, 1420, 2218
VspI ATTAAT 1 cut(s) 1376
XagI CCTNNNNNAGG 1 cut(s) 1307
XapI RAATTY 1 cut(s) 851
XbaI TCTAGA 1 cut(s) 1175
XceI RCATGY 2 cut(s) 1462, 1799
XcmI CCANNNNNNNNNTGG 1 cut(s) 1868
XmnI GAANNNNTTC 2 cut(s) 579, 880
Zsp2I ATGCAT 1 cut(s) 1790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.