Rh7DG466500

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0012349
Physical Location & Seq
Reverse (-)
66573747 .. 66578729
4983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG466500.1

Sequence Viewer

Length: 2220 bp
ATGTTGAGAATAGCTGAGCAAGACCAGAGGGTTTTGATGAACATGAAGAATGATAACAACAACAACAACAACCCAAGAATCCCTCCACCTTATTGTGATTCCAATCTTTTTCAGCTTCTGCTTCAGTCTAACCATCACCATGTGAGTGGTTCACCTATTAGCAGAGCTTCTCTGCAATCTGAGTTTTCTCCTTCGAGTTCCTTCTCAAATGGGTTTTATTCCTCTGAGGATAGCTCTCCTTATTCTCCAACCCCATTTGAGGAGGCAAAGTACCAAACACCTCTTAAGCATTACATGAATGGAGGAGGGTTGTGGTTGGACTCCAAGCCAGCACCACCACACCATGAGTCTCATTATAATGAGATTTTGGGGGATGATCTGGGGTTGGCTGAGAGTTTTTACAGGATGAATATGGGGGATGGACATGAGGGTGGTGCTAAAAGGAGGGGGTTTGAGAGAGATCCAGATGGGTTTGGGCTTGGTGGTGGTTTCTTAGGGGGTAATATTCCTTGGAATGTTGAGAACTATGGCTTGTTTGAAACTTCTAGGAACAATATGTCTGATGTTGAAGCTTTTCAGTCCTCTCATTTTGGTGTTAGAGGTGGGTTTAATGGTTATGTGGAGCCTGAGTTTCTTGGATTGGGGCCAGAATATGTTATTGGTGATTCAATGGGGGCTGTTCTAAATCATATGCAGCAGCCTAGTGCTTTGTGTTGTGGTCCTAGTGGTGCTAAGAACCAAATGGGTTACATGGTAGAGCAAAGAAAGGAACTGAGAGATGATTGGTATTACAGAAATGTTCAAGTAGAGAACCCATATGTTGCCAGACCTTACCTTGATGATGAATTTGTTTATTCACAGCTATGTGGAACAGATTCTAGTGCCGGAAAGGGTCTTATGTATCCGATGAGCTCTCCTCAGTTCCCCTCAGTTGCTCAGCTGTGTGTGGACAATAGTGCACATAATCATCCAGTGATAAAACAAAGGACTAGAGGAAATCCGACTAGGAAATGTGCAGGGCACTTAGAGGGTTTTAGAGGTGAGGATAGTCTTGCTGTCCAAGGAAAACATTTGAACCATGGTGTCACCAAGGGTAGCAAGTCTGCAAAGGCTAACAAGAAGAACTGTTGCACTAAGATGCCAATGACGACAGAAGGAGAGAAAAGCTCTAGACTAGATTTGGATTCATATAATGGAGGAAATGAATGTAGTCAGAGCAGTAATGACTCAATGAAGTCACTGCTTACTTTGGGTTCATTGACTGAGGTCCAGGGATACATATACTTCCTAGCAAAGGATCAGCATGGTTGCCGGTTCTTACAGAGAATGCTCGATGAGGGAACCCGTCAAGATGTGCAATTAATATTTGACAGGATAATCAATCATGTTCTTGAACTTATGACGGACCCGTTTGGCAATTACCTAATGCAGAAGTTCTTGGACATGTGTAATGAACAGCAAAGAATGCAGTTTGTACTTAAGGTGACCAAAGAACCGGGACAGCTTGTGAGAGTTTCCTTGGACACACATGGCACTCGTGTGGTGCAGAAGTTGATCGAGACTGTTGAAAATGGGAAACTGGTCTCTTTGATCATATCATCCCTTGAATGTGGTTTTCTTGATCTTATTAAGGATCCAAATGGCAATCATGTGGTACAGCGTTGCTTGGACTGCTTCAGCACTGAAGAGAACAGGTTTATATTTGATGCTGCTGCAAGATTCTGTGTTGAGATTGCAACTCAGCGTCATGGATGCTGTGTGCTACAAAAATGCATTGCACATGCCAGTGGGAGACATCGAGATAAGCTGGTCACTGAAGTTTCCAGGAATGGGCTTCTCCTTTCCCAGGATCCGTATGGAAACTATGTAGTTCAGTATGTCATAGAGCTGAAGATCCCATCTGTCATGGCCCGATTGATCTCTCAGTTGAAGGGACACTTTGTACTCCTCTCCATGCAGAAATGCAGTAGTCATGTTGTTGAAAAATGCCTCAAGCACTATGAAGAGAGCCGAGCAAAAGTCATCCAGGAATTGCTCTCAGTTTCTCACTTTGAACAGCTGTTGCAAGACCCTTATGCTAACTATGTCATTCAGTCTGCTCTTTCAGTTACCAAGGGCCCTCTTCACGCTGCACTGGTTGATGCTGTCAAGCCTCATACAATCTTGCGCCACAGCCCATATTGCAAGAGGATTTTCACAAAGGACCTCCTGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

739

Amino Acids

82.86

Weight (kDa)

6.75

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 427 - 460 4.3e-11 Pumilio-family RNA binding repeat
PUF PF00806 462 - 494 5.7e-09 Pumilio-family RNA binding repeat
PUF PF00806 539 - 569 3.6e-08 Pumilio-family RNA binding repeat
PUF PF00806 575 - 607 7.7e-13 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012349)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22240 AT1G78160 AT1G78160
fragaria_vesca FvH4_5g37620
malus_domestica MD08G1181900.v1.1 MD15G1367200.v1.1
prunus_persica Prupe.1G515100_v2.0.a1
pyrus_communis pycom08g15510 pycom15g32920
rosa_chinensis RchiOBHm_Chr7g0239301
rosa_laevigata RLG00000000765
rosa_multiflora Rmu_sc0007840.1_g000004
rosa_roxburghii Rroxscaffold_3G00221040
rosa_rugosa Rorug07G0327100
rosa_samantha Rh7AG480500 Rh7BG453200 Rh7CG498400 Rh7DG466500
rosa_wichuraiana Rw7G040250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 357
AclWI GGATC 7 cut(s) 455, 1305, 1628, 1641, 1844, 1857, 1888
AcsI RAATTY 1 cut(s) 845
AcuI CTGAAG 5 cut(s) 107, 1660, 1704, 1836, 1910
AdeI CACNNNGTG 1 cut(s) 142
AfaI GTAC 4 cut(s) 272, 1476, 1656, 1944
AfiI CCNNNNNNNGG 4 cut(s) 259, 1294, 1830, 1846
AflII CTTAAG 2 cut(s) 284, 1478
AflIII ACRYGT 1 cut(s) 1443
AjnI CCWGG 4 cut(s) 1269, 1823, 1845, 2025
AjuI GAANNNNNNNTTGG 2 cut(s) 642, 674
AleI CACNNNNGTG 1 cut(s) 1538
Alw21I GWGCWC 2 cut(s) 914, 961
Alw26I GTCTC 4 cut(s) 354, 1553, 1588, 1786
Alw44I GTGCAC 1 cut(s) 957
AlwI GGATC 7 cut(s) 455, 1305, 1628, 1641, 1844, 1857, 1888
AlwNI CAGNNNCTG 1 cut(s) 118
AoxI GGCC 3 cut(s) 644, 1908, 2116
ApaI GGGCCC 1 cut(s) 2120
ApaLI GTGCAC 1 cut(s) 957
ApeKI GCWGC 5 cut(s) 694, 697, 1709, 1712, 2129
ApoI RAATTY 1 cut(s) 845
AseI ATTAAT 1 cut(s) 1361
Asp700I GAANNNNTTC 2 cut(s) 573, 874
AspLEI GCGC 1 cut(s) 2169
AspS9I GGNCC 8 cut(s) 644, 719, 1267, 1405, 1909, 2116, 2117, 2203
AsuC2I CCSGG 1 cut(s) 1497
AsuHPI GGTGA 6 cut(s) 128, 144, 674, 1052, 1078, 1495
AvaII GGWCC 4 cut(s) 719, 1267, 1405, 2203
BaeGI GKGCMC 3 cut(s) 961, 1023, 2120
BamHI GGATCC 2 cut(s) 1633, 1849
BanII GRGCYC 2 cut(s) 914, 2120
BauI CACGAG 1 cut(s) 1536
Bbv12I GWGCWC 2 cut(s) 914, 961
BbvI GCAGC 5 cut(s) 706, 709, 1696, 1699, 2116
BccI CCATC 4 cut(s) 141, 413, 461, 1906
BciT130I CCWGG 4 cut(s) 1271, 1825, 1847, 2027
BciVI GTATCC 2 cut(s) 912, 1268
BclI TGATCA 1 cut(s) 1590
BcnI CCSGG 1 cut(s) 1497
BcoDI GTCTC 4 cut(s) 354, 1553, 1588, 1786
BfaI CTAG 9 cut(s) 546, 702, 723, 879, 990, 1005, 1170, 1175, 1289
BfrI CTTAAG 2 cut(s) 284, 1478
BfuI GTATCC 2 cut(s) 912, 1268
BisI GCNGC 5 cut(s) 695, 698, 1710, 1713, 2130
BlpI GCTNAGC 2 cut(s) 15, 936
BlsI GCNGC 5 cut(s) 696, 699, 1711, 1714, 2131
Bme1390I CCNGG 5 cut(s) 1271, 1497, 1825, 1847, 2027
Bme18I GGWCC 4 cut(s) 719, 1267, 1405, 2203
BmgT120I GGNCC 8 cut(s) 644, 719, 1267, 1405, 1909, 2116, 2117, 2203
BmiI GGNNCC 7 cut(s) 624, 645, 1342, 1407, 1635, 1851, 2118
BmrFI CCNGG 5 cut(s) 1271, 1497, 1825, 1847, 2027
BmsI GCATC 4 cut(s) 1128, 1696, 1742, 2131
BoxI GACNNNNGTC 1 cut(s) 1265
BplI GAGNNNNNCTC 6 cut(s) 218, 250, 901, 933, 1151, 1183
Bpu1102I GCTNAGC 2 cut(s) 15, 936
BpuEI CTTGAG 1 cut(s) 1976
BpuMI CCSGG 1 cut(s) 1497
BsaBI GATNNNNATC 1 cut(s) 102
BsaI GGTCTC 1 cut(s) 1588
BsaJI CCNNGG 8 cut(s) 509, 1060, 1078, 1089, 1270, 1518, 1845, 2112
BsaXI ACNNNNNCTCC 4 cut(s) 254, 284, 614, 644
Bsc4I CCNNNNNNNGG 4 cut(s) 259, 1294, 1830, 1846
Bse118I RCCGGY 1 cut(s) 1311
Bse1I ACTGG 4 cut(s) 971, 1584, 1785, 2139
Bse3DI GCAATG 1 cut(s) 1773
Bse8I GATNNNNATC 1 cut(s) 102
BseBI CCWGG 4 cut(s) 1271, 1825, 1847, 2027
BseDI CCNNGG 8 cut(s) 509, 1060, 1078, 1089, 1270, 1518, 1845, 2112
BseGI GGATG 7 cut(s) 379, 411, 424, 967, 1598, 1757, 2022
BseJI GATNNNNATC 1 cut(s) 102
BseLI CCNNNNNNNGG 4 cut(s) 259, 1294, 1830, 1846
BseMI GCAATG 1 cut(s) 1773
BseNI ACTGG 4 cut(s) 971, 1584, 1785, 2139
BseRI GAGGAG 4 cut(s) 275, 318, 906, 1937
BseSI GKGCMC 3 cut(s) 961, 1023, 2120
BseXI GCAGC 5 cut(s) 706, 709, 1696, 1699, 2116
BsgI GTGCAG 3 cut(s) 1035, 1565, 2115
BshFI GGCC 3 cut(s) 646, 1910, 2118
BsiHKAI GWGCWC 2 cut(s) 914, 961
BsiSI CCGG 3 cut(s) 885, 1312, 1496
BslFI GGGAC 2 cut(s) 1512, 1947
BslI CCNNNNNNNGG 4 cut(s) 259, 1294, 1830, 1846
BsmAI GTCTC 4 cut(s) 354, 1553, 1588, 1786
BsmFI GGGAC 2 cut(s) 1512, 1947
BsmI GAATGC 2 cut(s) 1332, 1470
BsnI GGCC 3 cut(s) 646, 1910, 2118
Bso31I GGTCTC 1 cut(s) 1588
Bsp120I GGGCCC 1 cut(s) 2116
Bsp1286I GDGCHC 4 cut(s) 914, 961, 1023, 2120
Bsp1720I GCTNAGC 2 cut(s) 15, 936
Bsp19I CCATGG 1 cut(s) 1078
BspANI GGCC 3 cut(s) 646, 1910, 2118
BspLI GGNNCC 7 cut(s) 624, 645, 1342, 1407, 1635, 1851, 2118
BspPI GGATC 7 cut(s) 455, 1305, 1628, 1641, 1844, 1857, 1888
BspTI CTTAAG 2 cut(s) 284, 1478
BspTNI GGTCTC 1 cut(s) 1588
BsrDI GCAATG 1 cut(s) 1773
BsrFI RCCGGY 1 cut(s) 1311
BsrI ACTGG 4 cut(s) 971, 1584, 1785, 2139
BssAI RCCGGY 1 cut(s) 1311
BssECI CCNNGG 8 cut(s) 509, 1060, 1078, 1089, 1270, 1518, 1845, 2112
BssSI CACGAG 1 cut(s) 1536
BssT1I CCWWGG 6 cut(s) 509, 1060, 1078, 1089, 1518, 2112
Bst2BI CACGAG 1 cut(s) 1536
Bst2UI CCWGG 4 cut(s) 1271, 1825, 1847, 2027
Bst4CI ACNGT 2 cut(s) 1127, 1564
Bst6I CTCTTC 3 cut(s) 1680, 1998, 2127
BstAFI CTTAAG 2 cut(s) 284, 1478
BstAPI GCANNNNNTGC 1 cut(s) 1465
BstC8I GCNNGC 1 cut(s) 330
BstDSI CCRYGG 1 cut(s) 1078
BstEII GGTNACC 1 cut(s) 1483
BstENI CCTNNNNNAGG 2 cut(s) 1292, 1844
BstF5I GGATG 7 cut(s) 379, 411, 424, 967, 1598, 1757, 2022
BstHHI GCGC 1 cut(s) 2169
BstMAI GTCTC 4 cut(s) 354, 1553, 1588, 1786
BstMWI GCNNNNNNNGC 3 cut(s) 1465, 1671, 2181
BstNI CCWGG 4 cut(s) 1271, 1825, 1847, 2027
BstNSI RCATGY 2 cut(s) 1447, 1784
BstPAI GACNNNNGTC 1 cut(s) 1265
BstPI GGTNACC 1 cut(s) 1483
BstSCI CCNGG 5 cut(s) 1269, 1495, 1823, 1845, 2025
BstSLI GKGCMC 3 cut(s) 961, 1023, 2120
BstV1I GCAGC 5 cut(s) 706, 709, 1696, 1699, 2116
BstX2I RGATCY 4 cut(s) 460, 1633, 1849, 1893
BstXI CCANNNNNNTGG 1 cut(s) 146
BstYI RGATCY 4 cut(s) 460, 1633, 1849, 1893
BsuI GTATCC 2 cut(s) 912, 1268
BsuRI GGCC 3 cut(s) 646, 1910, 2118
BtgI CCRYGG 1 cut(s) 1078
BtsCI GGATG 7 cut(s) 379, 411, 424, 967, 1598, 1757, 2022
BtsI GCAGTG 1 cut(s) 1238
BtsIMutI CAGTG 6 cut(s) 978, 1238, 1680, 1792, 1812, 2132
Cac8I GCNNGC 1 cut(s) 330
CaiI CAGNNNCTG 1 cut(s) 118
CfoI GCGC 1 cut(s) 2169
Cfr10I RCCGGY 1 cut(s) 1311
Cfr13I GGNCC 8 cut(s) 644, 719, 1267, 1405, 1909, 2116, 2117, 2203
CseI GACGC 1 cut(s) 1733
Csp6I GTAC 4 cut(s) 271, 1475, 1655, 1943
CviQI GTAC 4 cut(s) 271, 1475, 1655, 1943
DraIII CACNNNGTG 1 cut(s) 142
Eam1104I CTCTTC 3 cut(s) 1680, 1998, 2127
EarI CTCTTC 3 cut(s) 1680, 1998, 2127
Ecl136II GAGCTC 1 cut(s) 912
Eco130I CCWWGG 6 cut(s) 509, 1060, 1078, 1089, 1518, 2112
Eco24I GRGCYC 2 cut(s) 914, 2120
Eco31I GGTCTC 1 cut(s) 1588
Eco47I GGWCC 4 cut(s) 719, 1267, 1405, 2203
Eco53kI GAGCTC 1 cut(s) 912
Eco57I CTGAAG 5 cut(s) 107, 1660, 1704, 1836, 1910
Eco91I GGTNACC 1 cut(s) 1483
EcoICRI GAGCTC 1 cut(s) 912
EcoNI CCTNNNNNAGG 2 cut(s) 1292, 1844
EcoO109I RGGNCCY 3 cut(s) 2116, 2117, 2203
EcoO65I GGTNACC 1 cut(s) 1483
EcoRII CCWGG 4 cut(s) 1269, 1823, 1845, 2025
EcoT14I CCWWGG 6 cut(s) 509, 1060, 1078, 1089, 1518, 2112
EcoT22I ATGCAT 1 cut(s) 1775
EcoT38I GRGCYC 2 cut(s) 914, 2120
ErhI CCWWGG 6 cut(s) 509, 1060, 1078, 1089, 1518, 2112
FalI AAGNNNNNCTT 2 cut(s) 1922, 1954
FaqI GGGAC 2 cut(s) 1512, 1947
FauNDI CATATG 2 cut(s) 690, 817
FbaI TGATCA 1 cut(s) 1590
Fnu4HI GCNGC 5 cut(s) 695, 698, 1710, 1713, 2130
FokI GGATG 7 cut(s) 386, 418, 431, 954, 1585, 1764, 2009
FriOI GRGCYC 2 cut(s) 914, 2120
Fsp4HI GCNGC 5 cut(s) 695, 698, 1710, 1713, 2130
FspBI CTAG 9 cut(s) 546, 702, 723, 879, 990, 1005, 1170, 1175, 1289
GlaI GCGC 1 cut(s) 2168
GluI GCNGC 5 cut(s) 695, 698, 1710, 1713, 2130
HaeIII GGCC 3 cut(s) 646, 1910, 2118
HapII CCGG 3 cut(s) 885, 1312, 1496
HgaI GACGC 1 cut(s) 1733
HhaI GCGC 1 cut(s) 2169
Hin6I GCGC 1 cut(s) 2167
HinP1I GCGC 1 cut(s) 2167
HindIII AAGCTT 1 cut(s) 570
HinfI GANTC 9 cut(s) 78, 98, 320, 347, 665, 875, 1184, 1226, 1719
HpaII CCGG 3 cut(s) 885, 1312, 1496
HphI GGTGA 6 cut(s) 128, 144, 674, 1052, 1078, 1495
Hpy166II GTNNAC 3 cut(s) 152, 949, 959
Hpy188I TCNGA 6 cut(s) 181, 226, 562, 906, 1002, 1215
Hpy188III TCNNGA 8 cut(s) 464, 1170, 1349, 1391, 1558, 1619, 1799, 2209
Hpy8I GTNNAC 3 cut(s) 152, 949, 959
HpyAV CCTTC 4 cut(s) 201, 211, 1148, 1924
HpyCH4III ACNGT 2 cut(s) 1127, 1564
HpyF10VI GCNNNNNNNGC 3 cut(s) 1465, 1671, 2181
HspAI GCGC 1 cut(s) 2167
Ksp22I TGATCA 1 cut(s) 1590
LmnI GCTCC 1 cut(s) 622
Lsp1109I GCAGC 5 cut(s) 706, 709, 1696, 1699, 2116
LweI GCATC 4 cut(s) 1128, 1696, 1742, 2131
MaeI CTAG 9 cut(s) 546, 702, 723, 879, 990, 1005, 1170, 1175, 1289
MaeIII GTNAC 6 cut(s) 746, 1084, 1236, 1483, 1810, 2107
MboII GAAGA 6 cut(s) 58, 1132, 1697, 1903, 2015, 2114
MflI RGATCY 4 cut(s) 460, 1633, 1849, 1893
MhlI GDGCHC 4 cut(s) 914, 961, 1023, 2120
MluCI AATT 4 cut(s) 845, 1358, 1417, 2030
MlyI GAGTC 3 cut(s) 314, 356, 1220
MmeI TCCRAC 3 cut(s) 272, 297, 1025
Mph1103I ATGCAT 1 cut(s) 1775
MroXI GAANNNNTTC 2 cut(s) 573, 874
MseI TTAA 5 cut(s) 285, 609, 1361, 1479, 1629
MslI CAYNNNNRTG 9 cut(s) 138, 144, 357, 429, 591, 862, 1136, 1538, 1785
MspA1I CMGCKG 2 cut(s) 940, 2059
MspCI CTTAAG 2 cut(s) 284, 1478
MspI CCGG 3 cut(s) 885, 1312, 1496
MspR9I CCNGG 5 cut(s) 1271, 1497, 1825, 1847, 2027
Mva1269I GAATGC 2 cut(s) 1332, 1470
MvaI CCWGG 4 cut(s) 1271, 1825, 1847, 2027
MwoI GCNNNNNNNGC 3 cut(s) 1465, 1671, 2181
NciI CCSGG 1 cut(s) 1497
NcoI CCATGG 1 cut(s) 1078
NdeI CATATG 2 cut(s) 690, 817
NlaIV GGNNCC 7 cut(s) 624, 645, 1342, 1407, 1635, 1851, 2118
NmeAIII GCCGAG 1 cut(s) 2036
NmuCI GTSAC 4 cut(s) 1084, 1236, 1483, 1810
NsiI ATGCAT 1 cut(s) 1775
NspI RCATGY 2 cut(s) 1447, 1784
OliI CACNNNNGTG 1 cut(s) 1538
PciI ACATGT 1 cut(s) 1443
PctI GAATGC 2 cut(s) 1332, 1470
PdmI GAANNNNTTC 2 cut(s) 573, 874
PfeI GAWTC 6 cut(s) 78, 98, 665, 875, 1184, 1719
PfoI TCCNGGA 2 cut(s) 1823, 2025
PkrI GCNGC 5 cut(s) 696, 699, 1711, 1714, 2131
PleI GAGTC 3 cut(s) 314, 355, 1220
PpsI GAGTC 3 cut(s) 314, 355, 1220
PpuMI RGGWCCY 1 cut(s) 2203
PscI ACATGT 1 cut(s) 1443
PshAI GACNNNNGTC 1 cut(s) 1265
PshBI ATTAAT 1 cut(s) 1361
PsiI TTATAA 1 cut(s) 357
Psp124BI GAGCTC 1 cut(s) 914
Psp5II RGGWCCY 1 cut(s) 2203
Psp6I CCWGG 4 cut(s) 1269, 1823, 1845, 2025
PspEI GGTNACC 1 cut(s) 1483
PspGI CCWGG 4 cut(s) 1269, 1823, 1845, 2025
PspN4I GGNNCC 7 cut(s) 624, 645, 1342, 1407, 1635, 1851, 2118
PspOMI GGGCCC 1 cut(s) 2116
PspPI GGNCC 8 cut(s) 644, 719, 1267, 1405, 1909, 2116, 2117, 2203
PspPPI RGGWCCY 1 cut(s) 2203
PstNI CAGNNNCTG 1 cut(s) 118
PsuI RGATCY 4 cut(s) 460, 1633, 1849, 1893
PvuII CAGCTG 2 cut(s) 940, 2059
RsaI GTAC 4 cut(s) 272, 1476, 1656, 1944
RsaNI GTAC 4 cut(s) 271, 1475, 1655, 1943
RseI CAYNNNNRTG 9 cut(s) 138, 144, 357, 429, 591, 862, 1136, 1538, 1785
SacI GAGCTC 1 cut(s) 914
SaqAI TTAA 5 cut(s) 285, 609, 1361, 1479, 1629
SatI GCNGC 5 cut(s) 695, 698, 1710, 1713, 2130
Sau96I GGNCC 8 cut(s) 644, 719, 1267, 1405, 1909, 2116, 2117, 2203
SchI GAGTC 3 cut(s) 314, 356, 1220
ScrFI CCNGG 5 cut(s) 1271, 1497, 1825, 1847, 2027
SduI GDGCHC 4 cut(s) 914, 961, 1023, 2120
SfaNI GCATC 4 cut(s) 1128, 1696, 1742, 2131
SinI GGWCC 4 cut(s) 719, 1267, 1405, 2203
SmiMI CAYNNNNRTG 9 cut(s) 138, 144, 357, 429, 591, 862, 1136, 1538, 1785
SmlI CTYRAG 3 cut(s) 284, 1478, 1991
SmoI CTYRAG 3 cut(s) 284, 1478, 1991
Sse9I AATT 4 cut(s) 845, 1358, 1417, 2030
SspI AATATT 2 cut(s) 505, 1365
SspMI CTAG 9 cut(s) 546, 702, 723, 879, 990, 1005, 1170, 1175, 1289
SstI GAGCTC 1 cut(s) 914
StyD4I CCNGG 5 cut(s) 1269, 1495, 1823, 1845, 2025
StyI CCWWGG 6 cut(s) 509, 1060, 1078, 1089, 1518, 2112
TaaI ACNGT 2 cut(s) 1127, 1564
TaqI TCGA 4 cut(s) 194, 1332, 1557, 1798
TasI AATT 4 cut(s) 845, 1358, 1417, 2030
TatI WGTACW 2 cut(s) 1474, 1942
TfiI GAWTC 6 cut(s) 78, 98, 665, 875, 1184, 1719
Tru1I TTAA 5 cut(s) 285, 609, 1361, 1479, 1629
Tru9I TTAA 5 cut(s) 285, 609, 1361, 1479, 1629
TscAI CASTG 6 cut(s) 978, 1245, 1687, 1792, 1819, 2139
TseFI GTSAC 4 cut(s) 1084, 1236, 1483, 1810
TseI GCWGC 5 cut(s) 694, 697, 1709, 1712, 2129
Tsp45I GTSAC 4 cut(s) 1084, 1236, 1483, 1810
TspGWI ACGGA 2 cut(s) 1418, 1842
TspRI CASTG 6 cut(s) 978, 1245, 1687, 1792, 1819, 2139
Vha464I CTTAAG 2 cut(s) 284, 1478
VneI GTGCAC 1 cut(s) 957
VpaK11BI GGWCC 4 cut(s) 719, 1267, 1405, 2203
VspI ATTAAT 1 cut(s) 1361
XagI CCTNNNNNAGG 2 cut(s) 1292, 1844
XapI RAATTY 1 cut(s) 845
XbaI TCTAGA 1 cut(s) 1169
XceI RCATGY 2 cut(s) 1447, 1784
XcmI CCANNNNNNNNNTGG 1 cut(s) 1853
XmnI GAANNNNTTC 2 cut(s) 573, 874
XspI CTAG 9 cut(s) 546, 702, 723, 879, 990, 1005, 1170, 1175, 1289
Zsp2I ATGCAT 1 cut(s) 1775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.