MD15G1367200.v1.1

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012349
Physical Location & Seq
Forward (+)
44691574 .. 44696121
4548 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1367200.v1.1.491

Sequence Viewer

Length: 2157 bp
ATGATGTTCAAACTATCTGAGGAAGACAAGAGGGTTTTACAGAGGGAAGGAGGAGAGCTGGAAAAGCTCTGGAATGAGAATCCCACCAGGAATCAGCATTATTCCAGTCTTTTCCACCTCCTTCATCCTCCGCATCACCGCCACCTTCTCGGCGGCTCTCCGGTGAGCAGAGCCTCTCTGCCGTCGGAGTTCTTTCCGTCGAGCTCTTTCTCAAGTGGGTTATATTCCTCCGAGGACGGTTCTCCGTCTCTGTATTCAACCCCATTGGAAGAGGCAAAGTATCAAACACCTTACGTGAATGGGTTGTGGCTGGACTCTAAGCCGCCTGAGGATGGTCTGGGTTTGCCTGAGAGTTTCTATAGGCTGCATATTGGGGAGGAACAGGAGGGTGGGAATAAAAGGATGGGATTTGACAGAGGCCCGGATGGGATTGGACTCGGTGGTGGGTTTTTTGGGGGGAATAGTCCTTGGAATGCTGAAAACCATGGCCTATTTGAGAGTTCTAAGAACAATGTTTCGGATTTTGATGGTTTCCAACCTGCGGGATTCGGCCGTGGAGGGAGATATGATGGGAGTACGGAGTCAGCATTGCTTGGATTGCAGCCGAAATGCGCTGTTGGCGAATCAATGGGGTCTTTTTGTAATCGTGTGTACCCGAATGCATTGTATGGGCCTAGTGGTGTTAAAAGAATGGAACAGGGAGATGGCTGGTATCAGAGGAACCCATCTAGTGCTATGTCTTACCCTGATGAGGATTATTTTAGCTTACTACATGCAATGGGTTTTAATGGCGGAAAGAGTCTTACATATCCGATGACTTCCCCTCAGTTTCCCTCGGTTGAACAGTTGAATGTGGACAATCTTTCACATAACCATTCAATGATAAAACAATGGACTAGAGTGAATCCCACTAATGGGGTTCCTGAATCTTTCCAGTCCATGAATTGTGCAGGGGAACTTGAGGGTTACGGTTGCGAGGATAGTTTCATCATGCAAGGAAAACGGTTGAATCATGCCATCAATAAAGGTAGCAAGTCTTCGAAGGGATACAAGAACTCTTGCACTAAGATGGCAATGCAGACAGGAGAGAAAAGCTCTAGACTTGATCCCGATTCATATGATGGCGGAATGAGTGGAAATGAATGGAGCCAGAGTGACAATAGCTCGTTTAGGTCAGTACCAAGTTTAAGTTCATTGGCCGAGGTTCAGGGATATATATATTTCATTGCAAAGGATCAGTATGGCTGTCGGTTCTTACAAAGGATGCTTGATGAGGGGACCAGCCAAGATTTGCAATTAATATTTGACAAAATAATCAATCATGTTTTTGAACTTATGACGAACCCGTTTGGCAATTACCTAATGCAAAAGTTGTTGGATGTGTGCAATGAACAACAAAGAATGCAATTTGTAATCGGCGTGACAAAAGAACCTGGACAGCTTGTCAGAGTTTCCTTAGACACACATGGGACTCGTGTGGTACAGAAGTTGATCGAGACTGTGAGAACAGGGAAACAGACCGTTTTAGTTAAATCGTCCCTTGAATGCGGTTTTCTTGATCTTATTAAGGATCCAAATGGTAATCATGTGGTACAACGTTGCTTGGACTGCTTTAGCAACGAAGACAATAAGTTTATTTTTGATGCTGCTGCAAGATTTTGTGTTGAGATTGCAACTCAACGCCATGGATGCTGTGTACTACAAAAATGCATTGCACATGCCATTGGGAGACATCGAGATAAGCTGATCAATGAAATTTCAAGAAATGGGCTTCTCCTTTCGCAAGATCCTTATGGGAATTATGTTGTTCAATATATCATAGAGCTGAAGATCCCATCTGCCATGGCCAAACTGACTAATCAGTTAAAAGGAAATTTTGTACGCCTCTCCATGCAGAAGTGCAGTAGTCATGTTGTTGAAAAGTGCCTCAAGTATTTTGAAGAGAGCCGGCCAAAAATCATCCATGAATTGCTCTCAGTTCCGCGTTTTGATCAGTTGTTGCAAGACCCTTATGCCAACTATGTCATTCAATCTGCTCTTGGAGTTACCAAGGGCCCTCTTCATGCTGCTCTGGTCCAAGCAGTTAGGCCTCATACAATCTTACGCACCAGTCCATACTGCAAGAGGATTTTCTCAAAGGACCTGAAGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

719

Amino Acids

80.32

Weight (kDa)

7.29

Isoelectric Point (pI)

46.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 406 - 439 3.6e-11 Pumilio-family RNA binding repeat
PUF PF00806 441 - 469 8.4e-09 Pumilio-family RNA binding repeat
PUF PF00806 518 - 548 4.5e-08 Pumilio-family RNA binding repeat
PUF PF00806 554 - 586 3.2e-12 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012349)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22240 AT1G78160 AT1G78160
fragaria_vesca FvH4_5g37620
malus_domestica MD08G1181900.v1.1 MD15G1367200.v1.1
prunus_persica Prupe.1G515100_v2.0.a1
pyrus_communis pycom08g15510 pycom15g32920
rosa_chinensis RchiOBHm_Chr7g0239301
rosa_laevigata RLG00000000765
rosa_multiflora Rmu_sc0007840.1_g000004
rosa_roxburghii Rroxscaffold_3G00221040
rosa_rugosa Rorug07G0327100
rosa_samantha Rh7AG480500 Rh7BG453200 Rh7CG498400 Rh7DG466500
rosa_wichuraiana Rw7G040250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 547
AccII CGCG 1 cut(s) 1984
AciI CCGC 9 cut(s) 131, 139, 153, 323, 542, 792, 1125, 1548, 1982
AclI AACGTT 1 cut(s) 1597
AclWI GGATC 6 cut(s) 1100, 1242, 1565, 1578, 1781, 1825
AcoI YGGCCR 4 cut(s) 550, 1197, 1845, 1949
AcsI RAATTY 2 cut(s) 1755, 1873
AcuI CTGAAG 1 cut(s) 1847
AfaI GTAC 7 cut(s) 577, 653, 1179, 1482, 1593, 1698, 1881
AfiI CCNNNNNNNGG 5 cut(s) 332, 541, 751, 914, 915
AhdI GACNNNNNGTC 1 cut(s) 1442
AjnI CCWGG 2 cut(s) 86, 1432
AjuI GAANNNNNNNTTGG 2 cut(s) 1174, 1206
AluBI AGCT 9 cut(s) 58, 67, 204, 765, 1095, 1164, 1441, 1744, 1825
AluI AGCT 9 cut(s) 58, 67, 204, 765, 1095, 1164, 1441, 1744, 1825
Alw21I GWGCWC 1 cut(s) 206
Alw26I GTCTC 3 cut(s) 252, 1490, 1723
AlwI GGATC 6 cut(s) 1100, 1242, 1565, 1578, 1781, 1825
AoxI GGCC 9 cut(s) 418, 487, 550, 671, 1197, 1845, 1949, 2053, 2087
ApaI GGGCCC 1 cut(s) 2057
ApeKI GCWGC 5 cut(s) 364, 601, 1646, 1649, 2066
ApoI RAATTY 2 cut(s) 1755, 1873
AseI ATTAAT 1 cut(s) 1298
AspLEI GCGC 1 cut(s) 614
AspS9I GGNCC 7 cut(s) 419, 671, 1278, 2053, 2054, 2074, 2140
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 2 cut(s) 128, 175
AsuII TTCGAA 1 cut(s) 1040
AvaII GGWCC 3 cut(s) 1278, 2074, 2140
AxyI CCTNAGG 1 cut(s) 327
BaeGI GKGCMC 1 cut(s) 2057
BaeI ACNNNNGTAYC 2 cut(s) 1472, 1505
BalI TGGCCA 1 cut(s) 1847
BamHI GGATCC 1 cut(s) 1570
BanII GRGCYC 2 cut(s) 206, 2057
BarI GAAGNNNNNNTAC 2 cut(s) 1021, 1053
BauI CACGAG 1 cut(s) 1473
BbsI GAAGAC 3 cut(s) 30, 1029, 1629
Bbv12I GWGCWC 1 cut(s) 206
BbvI GCAGC 5 cut(s) 351, 613, 1633, 1636, 2053
BceAI ACGGC 2 cut(s) 166, 537
BciT130I CCWGG 2 cut(s) 88, 1434
BciVI GTATCC 1 cut(s) 1040
BclI TGATCA 2 cut(s) 1746, 1990
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 3 cut(s) 252, 1490, 1723
BfaI CTAG 4 cut(s) 675, 729, 897, 1098
BfmI CTRYAG 1 cut(s) 358
BfuAI ACCTGC 1 cut(s) 547
BfuI GTATCC 1 cut(s) 1040
BisI GCNGC 7 cut(s) 154, 323, 365, 602, 1647, 1650, 2067
BlsI GCNGC 7 cut(s) 155, 324, 366, 603, 1648, 1651, 2068
Bme1390I CCNGG 3 cut(s) 88, 422, 1434
Bme18I GGWCC 3 cut(s) 1278, 2074, 2140
BmeRI GACNNNNNGTC 1 cut(s) 1442
BmgT120I GGNCC 7 cut(s) 419, 671, 1278, 2053, 2054, 2074, 2140
BmiI GGNNCC 6 cut(s) 722, 921, 1148, 1279, 1572, 2055
BmrFI CCNGG 3 cut(s) 88, 422, 1434
BmsI GCATC 4 cut(s) 142, 1254, 1633, 1679
BpiI GAAGAC 3 cut(s) 30, 1029, 1629
BplI GAGNNNNNCTC 2 cut(s) 1079, 1111
Bpu14I TTCGAA 1 cut(s) 1040
BpuEI CTTGAG 3 cut(s) 196, 980, 1913
BpuMI CCSGG 1 cut(s) 422
BsaAI YACGTR 1 cut(s) 295
BsaJI CCNNGG 9 cut(s) 231, 467, 484, 553, 834, 1200, 1684, 1842, 2049
BsaWI WCCGGW 1 cut(s) 160
BsaXI ACNNNNNCTCC 2 cut(s) 1138, 1168
Bsc4I CCNNNNNNNGG 5 cut(s) 332, 541, 751, 914, 915
Bse118I RCCGGY 1 cut(s) 1947
Bse1I ACTGG 3 cut(s) 105, 934, 2109
Bse21I CCTNAGG 1 cut(s) 327
Bse3DI GCAATG 6 cut(s) 587, 783, 1080, 1224, 1393, 1710
BseBI CCWGG 2 cut(s) 88, 1434
BseDI CCNNGG 9 cut(s) 231, 467, 484, 553, 834, 1200, 1684, 1842, 2049
BseGI GGATG 8 cut(s) 124, 337, 408, 430, 1269, 1384, 1694, 1959
BseLI CCNNNNNNNGG 5 cut(s) 332, 541, 751, 914, 915
BseMI GCAATG 6 cut(s) 587, 783, 1080, 1224, 1393, 1710
BseMII CTCAG 5 cut(s) 9, 318, 339, 839, 1989
BseNI ACTGG 3 cut(s) 105, 934, 2109
BseRI GAGGAG 1 cut(s) 66
BseSI GKGCMC 1 cut(s) 2057
BseX3I CGGCCG 1 cut(s) 550
BseXI GCAGC 5 cut(s) 351, 613, 1633, 1636, 2053
BsgI GTGCAG 2 cut(s) 969, 1921
Bsh1236I CGCG 1 cut(s) 1984
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 9 cut(s) 420, 489, 552, 673, 1199, 1847, 1951, 2055, 2089
BsiEI CGRYCG 1 cut(s) 553
BsiHKAI GWGCWC 1 cut(s) 206
BsiSI CCGG 3 cut(s) 161, 422, 1948
BslFI GGGAC 3 cut(s) 1291, 1483, 1522
BslI CCNNNNNNNGG 5 cut(s) 332, 541, 751, 914, 915
BsmAI GTCTC 3 cut(s) 252, 1490, 1723
BsmBI CGTCTC 1 cut(s) 252
BsmFI GGGAC 3 cut(s) 1291, 1483, 1522
BsmI GAATGC 4 cut(s) 478, 664, 1407, 1550
BsnI GGCC 9 cut(s) 420, 489, 552, 673, 1199, 1847, 1951, 2055, 2089
Bsp119I TTCGAA 1 cut(s) 1040
Bsp120I GGGCCC 1 cut(s) 2053
Bsp1286I GDGCHC 2 cut(s) 206, 2057
Bsp143I GATC 9 cut(s) 1105, 1234, 1491, 1558, 1570, 1746, 1786, 1830, 1990
Bsp19I CCATGG 3 cut(s) 484, 1684, 1842
BspACI CCGC 9 cut(s) 131, 139, 153, 323, 542, 792, 1125, 1548, 1982
BspANI GGCC 9 cut(s) 420, 489, 552, 673, 1199, 1847, 1951, 2055, 2089
BspCNI CTCAG 5 cut(s) 10, 319, 340, 838, 1988
BspFNI CGCG 1 cut(s) 1984
BspLI GGNNCC 6 cut(s) 722, 921, 1148, 1279, 1572, 2055
BspMI ACCTGC 1 cut(s) 547
BspPI GGATC 6 cut(s) 1100, 1242, 1565, 1578, 1781, 1825
BspT104I TTCGAA 1 cut(s) 1040
BsrDI GCAATG 6 cut(s) 587, 783, 1080, 1224, 1393, 1710
BsrFI RCCGGY 1 cut(s) 1947
BsrI ACTGG 3 cut(s) 105, 934, 2109
BssAI RCCGGY 1 cut(s) 1947
BssECI CCNNGG 9 cut(s) 231, 467, 484, 553, 834, 1200, 1684, 1842, 2049
BssMI GATC 9 cut(s) 1105, 1234, 1491, 1558, 1570, 1746, 1786, 1830, 1990
BssSI CACGAG 1 cut(s) 1473
BssT1I CCWWGG 5 cut(s) 467, 484, 1684, 1842, 2049
Bst2BI CACGAG 1 cut(s) 1473
Bst2UI CCWGG 2 cut(s) 88, 1434
Bst4CI ACNGT 6 cut(s) 239, 846, 971, 1005, 1501, 1522
Bst6I CTCTTC 3 cut(s) 264, 1935, 2064
BstBAI YACGTR 1 cut(s) 295
BstBI TTCGAA 1 cut(s) 1040
BstC8I GCNNGC 1 cut(s) 1949
BstDEI CTNAG 9 cut(s) 18, 318, 327, 348, 504, 825, 1065, 1456, 1975
BstDSI CCRYGG 4 cut(s) 484, 553, 1684, 1842
BstF5I GGATG 8 cut(s) 124, 337, 408, 430, 1269, 1384, 1694, 1959
BstFNI CGCG 1 cut(s) 1984
BstHHI GCGC 1 cut(s) 614
BstKTI GATC 9 cut(s) 1108, 1237, 1494, 1561, 1573, 1749, 1789, 1833, 1993
BstMAI GTCTC 3 cut(s) 252, 1490, 1723
BstMBI GATC 9 cut(s) 1105, 1234, 1491, 1558, 1570, 1746, 1786, 1830, 1990
BstMCI CGRYCG 1 cut(s) 553
BstMWI GCNNNNNNNGC 5 cut(s) 64, 598, 618, 1608, 1689
BstNI CCWGG 2 cut(s) 88, 1434
BstNSI RCATGY 2 cut(s) 776, 1721
BstSCI CCNGG 3 cut(s) 86, 420, 1432
BstSFI CTRYAG 1 cut(s) 358
BstSLI GKGCMC 1 cut(s) 2057
BstUI CGCG 1 cut(s) 1984
BstV1I GCAGC 5 cut(s) 351, 613, 1633, 1636, 2053
BstV2I GAAGAC 3 cut(s) 30, 1029, 1629
BstX2I RGATCY 3 cut(s) 1570, 1786, 1830
BstYI RGATCY 3 cut(s) 1570, 1786, 1830
BstZI CGGCCG 1 cut(s) 550
Bsu36I CCTNAGG 1 cut(s) 327
BsuI GTATCC 1 cut(s) 1040
BsuRI GGCC 9 cut(s) 420, 489, 552, 673, 1199, 1847, 1951, 2055, 2089
BtgI CCRYGG 4 cut(s) 484, 553, 1684, 1842
BtsCI GGATG 8 cut(s) 124, 337, 408, 430, 1269, 1384, 1694, 1959
BveI ACCTGC 1 cut(s) 547
Cac8I GCNNGC 1 cut(s) 1949
CfoI GCGC 1 cut(s) 614
Cfr10I RCCGGY 1 cut(s) 1947
Cfr13I GGNCC 7 cut(s) 419, 671, 1278, 2053, 2054, 2074, 2140
Csp6I GTAC 7 cut(s) 576, 652, 1178, 1481, 1592, 1697, 1880
CviQI GTAC 7 cut(s) 576, 652, 1178, 1481, 1592, 1697, 1880
DdeI CTNAG 9 cut(s) 18, 318, 327, 348, 504, 825, 1065, 1456, 1975
DpnI GATC 9 cut(s) 1107, 1236, 1493, 1560, 1572, 1748, 1788, 1832, 1992
DpnII GATC 9 cut(s) 1105, 1234, 1491, 1558, 1570, 1746, 1786, 1830, 1990
DriI GACNNNNNGTC 1 cut(s) 1442
EaeI YGGCCR 4 cut(s) 550, 1197, 1845, 1949
EagI CGGCCG 1 cut(s) 550
Eam1104I CTCTTC 3 cut(s) 264, 1935, 2064
Eam1105I GACNNNNNGTC 1 cut(s) 1442
EarI CTCTTC 3 cut(s) 264, 1935, 2064
EciI GGCGGA 2 cut(s) 807, 1140
Ecl136II GAGCTC 1 cut(s) 204
EclXI CGGCCG 1 cut(s) 550
Eco130I CCWWGG 5 cut(s) 467, 484, 1684, 1842, 2049
Eco147I AGGCCT 1 cut(s) 2089
Eco24I GRGCYC 2 cut(s) 206, 2057
Eco47I GGWCC 3 cut(s) 1278, 2074, 2140
Eco52I CGGCCG 1 cut(s) 550
Eco53kI GAGCTC 1 cut(s) 204
Eco57I CTGAAG 1 cut(s) 1847
Eco81I CCTNAGG 1 cut(s) 327
EcoICRI GAGCTC 1 cut(s) 204
EcoO109I RGGNCCY 3 cut(s) 2053, 2054, 2140
EcoRII CCWGG 2 cut(s) 86, 1432
EcoT14I CCWWGG 5 cut(s) 467, 484, 1684, 1842, 2049
EcoT22I ATGCAT 2 cut(s) 664, 1712
EcoT38I GRGCYC 2 cut(s) 206, 2057
ErhI CCWWGG 5 cut(s) 467, 484, 1684, 1842, 2049
Esp3I CGTCTC 1 cut(s) 252
FaqI GGGAC 3 cut(s) 1291, 1483, 1522
FauI CCCGC 1 cut(s) 535
FauNDI CATATG 1 cut(s) 1117
FbaI TGATCA 2 cut(s) 1746, 1990
Fnu4HI GCNGC 7 cut(s) 154, 323, 365, 602, 1647, 1650, 2067
FokI GGATG 8 cut(s) 111, 344, 415, 437, 1276, 1391, 1701, 1946
FriOI GRGCYC 2 cut(s) 206, 2057
Fsp4HI GCNGC 7 cut(s) 154, 323, 365, 602, 1647, 1650, 2067
FspBI CTAG 4 cut(s) 675, 729, 897, 1098
GlaI GCGC 1 cut(s) 613
GluI GCNGC 7 cut(s) 154, 323, 365, 602, 1647, 1650, 2067
HaeIII GGCC 9 cut(s) 420, 489, 552, 673, 1199, 1847, 1951, 2055, 2089
HapII CCGG 3 cut(s) 161, 422, 1948
HhaI GCGC 1 cut(s) 614
Hin6I GCGC 1 cut(s) 612
HinP1I GCGC 1 cut(s) 612
HpaII CCGG 3 cut(s) 161, 422, 1948
HphI GGTGA 2 cut(s) 128, 175
Hpy166II GTNNAC 3 cut(s) 652, 856, 1697
Hpy188I TCNGA 7 cut(s) 19, 187, 232, 520, 717, 813, 1448
Hpy188III TCNNGA 8 cut(s) 70, 923, 1098, 1109, 1495, 1556, 1736, 1761
Hpy8I GTNNAC 3 cut(s) 652, 856, 1697
Hpy99I CGWCG 2 cut(s) 187, 202
HpyAV CCTTC 4 cut(s) 41, 131, 155, 1036
HpyCH4III ACNGT 6 cut(s) 239, 846, 971, 1005, 1501, 1522
HpyCH4IV ACGT 2 cut(s) 294, 1597
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 598, 618, 1608, 1689
HpyF3I CTNAG 9 cut(s) 18, 318, 327, 348, 504, 825, 1065, 1456, 1975
HpySE526I ACGT 2 cut(s) 294, 1597
HspAI GCGC 1 cut(s) 612
KroI GCCGGC 1 cut(s) 1947
KroNI GCCGGC 1 cut(s) 1949
Ksp22I TGATCA 2 cut(s) 1746, 1990
Kzo9I GATC 9 cut(s) 1105, 1234, 1491, 1558, 1570, 1746, 1786, 1830, 1990
LmnI GCTCC 1 cut(s) 1146
Lsp1109I GCAGC 5 cut(s) 351, 613, 1633, 1636, 2053
LweI GCATC 4 cut(s) 142, 1254, 1633, 1679
MaeI CTAG 4 cut(s) 675, 729, 897, 1098
MaeII ACGT 2 cut(s) 294, 1597
MaeIII GTNAC 4 cut(s) 965, 1154, 1420, 2044
MalI GATC 9 cut(s) 1107, 1236, 1493, 1560, 1572, 1748, 1788, 1832, 1992
MboI GATC 9 cut(s) 1105, 1234, 1491, 1558, 1570, 1746, 1786, 1830, 1990
MboII GAAGA 7 cut(s) 35, 281, 1029, 1634, 1840, 1952, 2051
MflI RGATCY 3 cut(s) 1570, 1786, 1830
MhlI GDGCHC 2 cut(s) 206, 2057
MlsI TGGCCA 1 cut(s) 1847
MluCI AATT 8 cut(s) 943, 1295, 1354, 1406, 1755, 1798, 1873, 1967
MluNI TGGCCA 1 cut(s) 1847
MlyI GAGTC 5 cut(s) 308, 429, 590, 808, 1465
MmeI TCCRAC 3 cut(s) 165, 559, 1356
Mox20I TGGCCA 1 cut(s) 1847
Mph1103I ATGCAT 2 cut(s) 664, 1712
MroNI GCCGGC 1 cut(s) 1947
MscI TGGCCA 1 cut(s) 1847
MseI TTAA 7 cut(s) 684, 786, 1187, 1298, 1530, 1566, 1865
MslI CAYNNNNRTG 1 cut(s) 1067
Msp20I TGGCCA 1 cut(s) 1847
MspI CCGG 3 cut(s) 161, 422, 1948
MspR9I CCNGG 3 cut(s) 88, 422, 1434
Mva1269I GAATGC 4 cut(s) 478, 664, 1407, 1550
MvaI CCWGG 2 cut(s) 88, 1434
MvnI CGCG 1 cut(s) 1984
MwoI GCNNNNNNNGC 5 cut(s) 64, 598, 618, 1608, 1689
NaeI GCCGGC 1 cut(s) 1949
NciI CCSGG 1 cut(s) 422
NcoI CCATGG 3 cut(s) 484, 1684, 1842
NdeI CATATG 1 cut(s) 1117
NdeII GATC 9 cut(s) 1105, 1234, 1491, 1558, 1570, 1746, 1786, 1830, 1990
NgoMIV GCCGGC 1 cut(s) 1947
NlaIV GGNNCC 6 cut(s) 722, 921, 1148, 1279, 1572, 2055
NmeAIII GCCGAG 2 cut(s) 129, 1225
NmuCI GTSAC 2 cut(s) 1154, 1420
NsiI ATGCAT 2 cut(s) 664, 1712
NspI RCATGY 2 cut(s) 776, 1721
NspV TTCGAA 1 cut(s) 1040
PceI AGGCCT 1 cut(s) 2089
PctI GAATGC 4 cut(s) 478, 664, 1407, 1550
PdiI GCCGGC 1 cut(s) 1949
PfeI GAWTC 8 cut(s) 79, 91, 546, 623, 904, 926, 1009, 1112
PkrI GCNGC 7 cut(s) 155, 324, 366, 603, 1648, 1651, 2068
PleI GAGTC 5 cut(s) 308, 429, 589, 807, 1465
PpsI GAGTC 5 cut(s) 308, 429, 589, 807, 1465
Ppu21I YACGTR 1 cut(s) 295
PpuMI RGGWCCY 1 cut(s) 2140
PshBI ATTAAT 1 cut(s) 1298
Psp124BI GAGCTC 1 cut(s) 206
Psp1406I AACGTT 1 cut(s) 1597
Psp5II RGGWCCY 1 cut(s) 2140
Psp6I CCWGG 2 cut(s) 86, 1432
PspGI CCWGG 2 cut(s) 86, 1432
PspN4I GGNNCC 6 cut(s) 722, 921, 1148, 1279, 1572, 2055
PspOMI GGGCCC 1 cut(s) 2053
PspPI GGNCC 7 cut(s) 419, 671, 1278, 2053, 2054, 2074, 2140
PspPPI RGGWCCY 1 cut(s) 2140
PsuI RGATCY 3 cut(s) 1570, 1786, 1830
RsaI GTAC 7 cut(s) 577, 653, 1179, 1482, 1593, 1698, 1881
RsaNI GTAC 7 cut(s) 576, 652, 1178, 1481, 1592, 1697, 1880
RseI CAYNNNNRTG 1 cut(s) 1067
SacI GAGCTC 1 cut(s) 206
SaqAI TTAA 7 cut(s) 684, 786, 1187, 1298, 1530, 1566, 1865
SatI GCNGC 7 cut(s) 154, 323, 365, 602, 1647, 1650, 2067
Sau3AI GATC 9 cut(s) 1105, 1234, 1491, 1558, 1570, 1746, 1786, 1830, 1990
Sau96I GGNCC 7 cut(s) 419, 671, 1278, 2053, 2054, 2074, 2140
SchI GAGTC 5 cut(s) 308, 429, 590, 808, 1465
ScrFI CCNGG 3 cut(s) 88, 422, 1434
SduI GDGCHC 2 cut(s) 206, 2057
SfaNI GCATC 4 cut(s) 142, 1254, 1633, 1679
SfcI CTRYAG 1 cut(s) 358
SfuI TTCGAA 1 cut(s) 1040
SinI GGWCC 3 cut(s) 1278, 2074, 2140
SmiMI CAYNNNNRTG 1 cut(s) 1067
SmlI CTYRAG 3 cut(s) 211, 959, 1928
SmoI CTYRAG 3 cut(s) 211, 959, 1928
Sse9I AATT 8 cut(s) 943, 1295, 1354, 1406, 1755, 1798, 1873, 1967
SseBI AGGCCT 1 cut(s) 2089
SsiI CCGC 9 cut(s) 131, 139, 153, 323, 542, 792, 1125, 1548, 1982
SspI AATATT 1 cut(s) 1302
SspMI CTAG 4 cut(s) 675, 729, 897, 1098
SstI GAGCTC 1 cut(s) 206
StuI AGGCCT 1 cut(s) 2089
StyD4I CCNGG 3 cut(s) 86, 420, 1432
StyI CCWWGG 5 cut(s) 467, 484, 1684, 1842, 2049
TaaI ACNGT 6 cut(s) 239, 846, 971, 1005, 1501, 1522
TaiI ACGT 2 cut(s) 297, 1600
TaqI TCGA 4 cut(s) 200, 1040, 1494, 1735
TasI AATT 8 cut(s) 943, 1295, 1354, 1406, 1755, 1798, 1873, 1967
TatI WGTACW 1 cut(s) 1696
TauI GCSGC 2 cut(s) 156, 325
TfiI GAWTC 8 cut(s) 79, 91, 546, 623, 904, 926, 1009, 1112
Tru1I TTAA 7 cut(s) 684, 786, 1187, 1298, 1530, 1566, 1865
Tru9I TTAA 7 cut(s) 684, 786, 1187, 1298, 1530, 1566, 1865
TseFI GTSAC 2 cut(s) 1154, 1420
TseI GCWGC 5 cut(s) 364, 601, 1646, 1649, 2066
Tsp45I GTSAC 2 cut(s) 1154, 1420
TspGWI ACGGA 3 cut(s) 186, 234, 593
VpaK11BI GGWCC 3 cut(s) 1278, 2074, 2140
VspI ATTAAT 1 cut(s) 1298
XapI RAATTY 2 cut(s) 1755, 1873
XbaI TCTAGA 1 cut(s) 1097
XceI RCATGY 2 cut(s) 776, 1721
XspI CTAG 4 cut(s) 675, 729, 897, 1098
Zsp2I ATGCAT 2 cut(s) 664, 1712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.