AT1G22510

Protein of unknown function (DUF1232)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007874
Physical Location & Seq
Reverse (-)
7950818 .. 7952832
2015 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G22510.1

Sequence Viewer

Length: 558 bp
ATGAATGCGCCACCGGAGAACGAACTTTGTTCGATATGCCATGGCCACTTCAACGCTCCTTGCCAATCCAATTGCTCTCACTGGTTCTGCGGGAACTGCATTATGCTAGTTTGGAGGCATGGATCTACATTACGGCCATGCAAATGTCCCTTGTGTCGTCGTCCGATAAGTTTGTTAGTTCCTTCTGAGGAAACAGTGAGAAGTCGGAATGATGCTACAGTTTCTGAGGTTCTTCATGATGTTGAAACTTACAACCGTGTCTTTGGTGGACAGTCAAGTGGCCTTATTCAGAGGATGCAAGACCTTCCCTTCTTACTCCGAAGGTTGTTGCGAGAAATGATGGATCCACAAAGAACGCTTCCACTCGTCATCAGAGCTCGTGTTTATATTGCGTTGATACTCAGTGCGATTTACATAATCAGCCCAATAGATATCATTCCGGAAGGAGTCTTGGGGGTTATCGGTTTACTAGATGATCTTCTCATAGCCCTGATTTGTTTCCTCCATGTCGCTGCTCTCTACCGATCCGTTCTCTATTCCCGTCATGGTGGTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0007275 GO:0008150 GO:0009790 GO:0009792 GO:0032501 GO:0032502 GO:0043009 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1232 zf-C3HC4_3

Protein Analysis

185

Amino Acids

20.78

Weight (kDa)

8.08

Isoelectric Point (pI)

53.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1232 PF06803 130 - 164 4.3e-09 Protein of unknown function (DUF1232)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 1 cut(s) 90
AclWI GGATC 4 cut(s) 130, 338, 351, 519
AcoI YGGCCR 2 cut(s) 43, 134
AgsI TTSAA 2 cut(s) 52, 245
AluBI AGCT 1 cut(s) 377
AluI AGCT 1 cut(s) 377
Alw21I GWGCWC 1 cut(s) 379
AlwI GGATC 4 cut(s) 130, 338, 351, 519
AlwNI CAGNNNCTG 1 cut(s) 224
Aor13HI TCCGGA 1 cut(s) 439
AoxI GGCC 3 cut(s) 43, 134, 280
ApeKI GCWGC 1 cut(s) 512
AspLEI GCGC 1 cut(s) 10
BalI TGGCCA 1 cut(s) 45
BamHI GGATCC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 379
BauI CACGAG 1 cut(s) 378
Bbv12I GWGCWC 1 cut(s) 379
BbvI GCAGC 1 cut(s) 499
BccI CCATC 1 cut(s) 334
BceAI ACGGC 1 cut(s) 149
BfaI CTAG 2 cut(s) 107, 470
BfmI CTRYAG 1 cut(s) 216
BisI GCNGC 1 cut(s) 513
BlsI GCNGC 1 cut(s) 514
BmiI GGNNCC 1 cut(s) 345
BmsI GCATC 2 cut(s) 202, 285
BsaJI CCNNGG 1 cut(s) 40
BsaWI WCCGGW 2 cut(s) 13, 439
Bse1I ACTGG 1 cut(s) 86
BseAI TCCGGA 1 cut(s) 439
BseDI CCNNGG 1 cut(s) 40
BseGI GGATG 1 cut(s) 300
BseMII CTCAG 3 cut(s) 177, 216, 415
BseNI ACTGG 1 cut(s) 86
BseXI GCAGC 1 cut(s) 499
BshFI GGCC 3 cut(s) 45, 136, 282
BsiHKAI GWGCWC 1 cut(s) 379
BsiSI CCGG 2 cut(s) 14, 440
BslFI GGGAC 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 132
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 3 cut(s) 45, 136, 282
Bsp1286I GDGCHC 1 cut(s) 379
Bsp13I TCCGGA 1 cut(s) 439
Bsp143I GATC 4 cut(s) 122, 343, 475, 524
Bsp19I CCATGG 1 cut(s) 40
BspACI CCGC 1 cut(s) 90
BspANI GGCC 3 cut(s) 45, 136, 282
BspCNI CTCAG 3 cut(s) 178, 217, 414
BspEI TCCGGA 1 cut(s) 439
BspHI TCATGA 1 cut(s) 235
BspLI GGNNCC 1 cut(s) 345
BspPI GGATC 4 cut(s) 130, 338, 351, 519
BsrI ACTGG 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 40
BssMI GATC 4 cut(s) 122, 343, 475, 524
BssSI CACGAG 1 cut(s) 378
BssT1I CCWWGG 1 cut(s) 40
Bst2BI CACGAG 1 cut(s) 378
Bst4CI ACNGT 4 cut(s) 196, 220, 257, 273
BstDEI CTNAG 3 cut(s) 186, 225, 401
BstDSI CCRYGG 1 cut(s) 40
BstF5I GGATG 1 cut(s) 300
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 4 cut(s) 125, 346, 478, 527
BstMBI GATC 4 cut(s) 122, 343, 475, 524
BstMWI GCNNNNNNNGC 1 cut(s) 96
BstSFI CTRYAG 1 cut(s) 216
BstV1I GCAGC 1 cut(s) 499
BstX2I RGATCY 2 cut(s) 122, 343
BstYI RGATCY 2 cut(s) 122, 343
BsuRI GGCC 3 cut(s) 45, 136, 282
BtgI CCRYGG 1 cut(s) 40
BtsCI GGATG 1 cut(s) 300
BtsIMutI CAGTG 3 cut(s) 79, 201, 409
CaiI CAGNNNCTG 1 cut(s) 224
CciI TCATGA 1 cut(s) 235
CfoI GCGC 1 cut(s) 10
CviAII CATG 6 cut(s) 41, 119, 138, 236, 506, 545
CviJI RGCY 6 cut(s) 45, 136, 282, 377, 423, 488
CviKI_1 RGCY 6 cut(s) 45, 136, 282, 377, 423, 488
DdeI CTNAG 3 cut(s) 186, 225, 401
DpnI GATC 4 cut(s) 124, 345, 477, 526
DpnII GATC 4 cut(s) 122, 343, 475, 524
EaeI YGGCCR 2 cut(s) 43, 134
Ecl136II GAGCTC 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 40
Eco24I GRGCYC 1 cut(s) 379
Eco32I GATATC 1 cut(s) 433
Eco53kI GAGCTC 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 377
EcoRV GATATC 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 40
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 6 cut(s) 44, 122, 141, 239, 509, 548
FaqI GGGAC 1 cut(s) 132
FatI CATG 6 cut(s) 40, 118, 137, 235, 505, 544
FauI CCCGC 1 cut(s) 83
Fnu4HI GCNGC 1 cut(s) 513
FokI GGATG 1 cut(s) 307
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 1 cut(s) 513
FspBI CTAG 2 cut(s) 107, 470
GlaI GCGC 1 cut(s) 9
GluI GCNGC 1 cut(s) 513
HaeIII GGCC 3 cut(s) 45, 136, 282
HapII CCGG 2 cut(s) 14, 440
HhaI GCGC 1 cut(s) 10
Hin1II CATG 6 cut(s) 44, 122, 141, 239, 509, 548
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HinfI GANTC 1 cut(s) 447
HpaII CCGG 2 cut(s) 14, 440
Hpy166II GTNNAC 2 cut(s) 269, 467
Hpy188I TCNGA 7 cut(s) 165, 187, 207, 226, 291, 320, 374
Hpy188III TCNNGA 3 cut(s) 236, 440, 555
Hpy8I GTNNAC 2 cut(s) 269, 467
Hpy99I CGWCG 1 cut(s) 162
HpyAV CCTTC 5 cut(s) 192, 314, 315, 319, 437
HpyCH4III ACNGT 4 cut(s) 196, 220, 257, 273
HpyCH4V TGCA 3 cut(s) 99, 141, 298
HpyF10VI GCNNNNNNNGC 1 cut(s) 96
HpyF3I CTNAG 3 cut(s) 186, 225, 401
Hsp92II CATG 6 cut(s) 44, 122, 141, 239, 509, 548
HspAI GCGC 1 cut(s) 8
Kpn2I TCCGGA 1 cut(s) 439
Kzo9I GATC 4 cut(s) 122, 343, 475, 524
LmnI GCTCC 1 cut(s) 61
LpnPI CCDG 4 cut(s) 27, 67, 453, 503
Lsp1109I GCAGC 1 cut(s) 499
LweI GCATC 2 cut(s) 202, 285
MaeI CTAG 2 cut(s) 107, 470
MalI GATC 4 cut(s) 124, 345, 477, 526
MboI GATC 4 cut(s) 122, 343, 475, 524
MboII GAAGA 2 cut(s) 224, 470
MfeI CAATTG 1 cut(s) 70
MflI RGATCY 2 cut(s) 122, 343
MhlI GDGCHC 1 cut(s) 379
MlsI TGGCCA 1 cut(s) 45
MluCI AATT 1 cut(s) 70
MluNI TGGCCA 1 cut(s) 45
MlyI GAGTC 1 cut(s) 456
MmeI TCCRAC 1 cut(s) 185
MnlI CCTC 5 cut(s) 108, 181, 220, 285, 512
Mox20I TGGCCA 1 cut(s) 45
MroI TCCGGA 1 cut(s) 439
MscI TGGCCA 1 cut(s) 45
MslI CAYNNNNRTG 1 cut(s) 142
Msp20I TGGCCA 1 cut(s) 45
MspI CCGG 2 cut(s) 14, 440
MunI CAATTG 1 cut(s) 70
Mva1269I GAATGC 1 cut(s) 10
MwoI GCNNNNNNNGC 1 cut(s) 96
NcoI CCATGG 1 cut(s) 40
NdeII GATC 4 cut(s) 122, 343, 475, 524
NlaIII CATG 6 cut(s) 44, 122, 141, 239, 509, 548
NlaIV GGNNCC 1 cut(s) 345
PagI TCATGA 1 cut(s) 235
PctI GAATGC 1 cut(s) 10
PkrI GCNGC 1 cut(s) 514
PleI GAGTC 1 cut(s) 455
PpsI GAGTC 1 cut(s) 455
Psp124BI GAGCTC 1 cut(s) 379
PspN4I GGNNCC 1 cut(s) 345
PstNI CAGNNNCTG 1 cut(s) 224
PsuI RGATCY 2 cut(s) 122, 343
RseI CAYNNNNRTG 1 cut(s) 142
SacI GAGCTC 1 cut(s) 379
SatI GCNGC 1 cut(s) 513
Sau3AI GATC 4 cut(s) 122, 343, 475, 524
SchI GAGTC 1 cut(s) 456
SduI GDGCHC 1 cut(s) 379
SetI ASST 4 cut(s) 231, 306, 326, 379
SfaNI GCATC 2 cut(s) 202, 285
SfcI CTRYAG 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 142
Sse9I AATT 1 cut(s) 70
SsiI CCGC 1 cut(s) 90
SspMI CTAG 2 cut(s) 107, 470
SstI GAGCTC 1 cut(s) 379
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 4 cut(s) 196, 220, 257, 273
TaqI TCGA 1 cut(s) 32
TasI AATT 1 cut(s) 70
TscAI CASTG 3 cut(s) 86, 201, 409
TseI GCWGC 1 cut(s) 512
TspDTI ATGAA 2 cut(s) 17, 224
TspGWI ACGGA 1 cut(s) 517
TspRI CASTG 3 cut(s) 86, 201, 409
XspI CTAG 2 cut(s) 107, 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.