FvH4_5g31590

E3 ubiquitin-protein ligase RNF170-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007874
Physical Location & Seq
Reverse (-)
22754791 .. 22761197
6407 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g31590.t1

Sequence Viewer

Length: 558 bp
ATGGACGGTCCTCCTGAAAACGATCAGTGCTCCGTCTGCCACAGCGGCTTCAACACTCCATGCCAAGCCAATTGCTCTCACTGGTTTTGCGGAAAGTGTATTATGCTTGTTTGGGATCACGGATCAGCTCTTCAGCCTTGTAAATGCCCTCTCTGTCGTCGCCAGATAACCTTGCTGGTTCCAACTGAGGCTTCATTGAGCCAACGTAATACGCCTGAGGTGGCTGAGATTCTAGGCAAGGTTGAAACGTATAATCGCTATTTTGGAGGTCAATCCAGAGGACTTATTCAGAGAATGCAAGACCTTCCTTTTCTTTTGCGGAGGCTGTTACGAGAAGTATTGGATCCGCGTAGATCGCTTCCCCTTGTTATAAGGGCTCGTGTCATTATTGCAACGATACTAACTGGTGCCTATATACTCAGTCCTGTTGACTTTATTCCGGAAGCAATATTTGGTATAGTTGGTCTGTTAGATGATCTCCTCATCACACTAGTGGTCTTCCTCCACATTGCTACCATTTATCAATCGGTACTCTATCAACGCCATGGAGGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1232 zf-C3HC4 zf-C3HC4_3

Protein Analysis

186

Amino Acids

20.72

Weight (kDa)

8.1

Isoelectric Point (pI)

49.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1232 PF06803 129 - 163 1.3e-08 Protein of unknown function (DUF1232)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 371
AccB1I GGYRCC 1 cut(s) 407
AccII CGCG 1 cut(s) 349
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 4 cut(s) 45, 90, 319, 347
AclWI GGATC 4 cut(s) 123, 130, 338, 351
AcuI CTGAAG 1 cut(s) 116
AfaI GTAC 1 cut(s) 531
AgsI TTSAA 2 cut(s) 52, 245
AhlI ACTAGT 1 cut(s) 490
AjuI GAANNNNNNNTTGG 4 cut(s) 175, 207, 435, 467
AleI CACNNNNGTG 1 cut(s) 491
AluBI AGCT 1 cut(s) 128
AluI AGCT 1 cut(s) 128
Alw21I GWGCWC 1 cut(s) 32
AlwI GGATC 4 cut(s) 123, 130, 338, 351
Aor13HI TCCGGA 1 cut(s) 439
AspS9I GGNCC 1 cut(s) 8
AvaII GGWCC 1 cut(s) 8
AxyI CCTNAGG 1 cut(s) 216
BamHI GGATCC 1 cut(s) 343
BanI GGYRCC 1 cut(s) 407
BanII GRGCYC 1 cut(s) 379
BauI CACGAG 1 cut(s) 378
BbsI GAAGAC 1 cut(s) 490
Bbv12I GWGCWC 1 cut(s) 32
BcuI ACTAGT 1 cut(s) 490
BfaI CTAG 2 cut(s) 233, 491
BglI GCCNNNNNGGC 1 cut(s) 45
BisI GCNGC 1 cut(s) 46
BlsI GCNGC 1 cut(s) 47
Bme18I GGWCC 1 cut(s) 8
BmgT120I GGNCC 1 cut(s) 8
BmiI GGNNCC 3 cut(s) 180, 345, 409
BpiI GAAGAC 1 cut(s) 490
BsaJI CCNNGG 1 cut(s) 544
BsaWI WCCGGW 1 cut(s) 439
Bse1I ACTGG 2 cut(s) 86, 409
Bse21I CCTNAGG 1 cut(s) 216
Bse3DI GCAATG 1 cut(s) 507
BseAI TCCGGA 1 cut(s) 439
BseDI CCNNGG 1 cut(s) 544
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 4 cut(s) 177, 207, 216, 433
BseNI ACTGG 2 cut(s) 86, 409
BseRI GAGGAG 1 cut(s) 470
Bsh1236I CGCG 1 cut(s) 349
BshNI GGYRCC 1 cut(s) 407
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 1 cut(s) 440
BsmI GAATGC 1 cut(s) 300
Bsp1286I GDGCHC 2 cut(s) 32, 379
Bsp13I TCCGGA 1 cut(s) 439
Bsp143I GATC 6 cut(s) 22, 115, 122, 343, 353, 475
Bsp19I CCATGG 1 cut(s) 544
BspACI CCGC 4 cut(s) 45, 90, 319, 347
BspCNI CTCAG 4 cut(s) 178, 208, 217, 432
BspEI TCCGGA 1 cut(s) 439
BspFNI CGCG 1 cut(s) 349
BspLI GGNNCC 3 cut(s) 180, 345, 409
BspPI GGATC 4 cut(s) 123, 130, 338, 351
BspQI GCTCTTC 1 cut(s) 135
BspT107I GGYRCC 1 cut(s) 407
BsrDI GCAATG 1 cut(s) 507
BsrI ACTGG 2 cut(s) 86, 409
BssECI CCNNGG 1 cut(s) 544
BssMI GATC 6 cut(s) 22, 115, 122, 343, 353, 475
BssSI CACGAG 1 cut(s) 378
BssT1I CCWWGG 1 cut(s) 544
Bst2BI CACGAG 1 cut(s) 378
Bst4CI ACNGT 1 cut(s) 8
Bst6I CTCTTC 1 cut(s) 135
BstDEI CTNAG 4 cut(s) 186, 216, 225, 419
BstDSI CCRYGG 1 cut(s) 544
BstFNI CGCG 1 cut(s) 349
BstKTI GATC 6 cut(s) 25, 118, 125, 346, 356, 478
BstMBI GATC 6 cut(s) 22, 115, 122, 343, 353, 475
BstMWI GCNNNNNNNGC 3 cut(s) 36, 45, 355
BstUI CGCG 1 cut(s) 349
BstV2I GAAGAC 1 cut(s) 490
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
Bsu36I CCTNAGG 1 cut(s) 216
BtgI CCRYGG 1 cut(s) 544
BtsIMutI CAGTG 2 cut(s) 32, 79
Cfr13I GGNCC 1 cut(s) 8
Csp6I GTAC 1 cut(s) 530
CviAII CATG 2 cut(s) 60, 545
CviJI RGCY 9 cut(s) 48, 68, 128, 136, 191, 201, 224, 325, 377
CviKI_1 RGCY 9 cut(s) 48, 68, 128, 136, 191, 201, 224, 325, 377
CviQI GTAC 1 cut(s) 530
DdeI CTNAG 4 cut(s) 186, 216, 225, 419
DpnI GATC 6 cut(s) 24, 117, 124, 345, 355, 477
DpnII GATC 6 cut(s) 22, 115, 122, 343, 353, 475
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
Eco130I CCWWGG 1 cut(s) 544
Eco24I GRGCYC 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 8
Eco57I CTGAAG 1 cut(s) 116
Eco81I CCTNAGG 1 cut(s) 216
EcoT14I CCWWGG 1 cut(s) 544
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 544
FaeI CATG 2 cut(s) 63, 548
FaiI YATR 8 cut(s) 61, 104, 252, 371, 414, 416, 458, 546
FatI CATG 2 cut(s) 59, 544
Fnu4HI GCNGC 1 cut(s) 46
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 1 cut(s) 46
FspBI CTAG 2 cut(s) 233, 491
GluI GCNGC 1 cut(s) 46
HapII CCGG 1 cut(s) 440
Hin1II CATG 2 cut(s) 63, 548
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HinfI GANTC 1 cut(s) 229
HpaII CCGG 1 cut(s) 440
Hpy166II GTNNAC 1 cut(s) 430
Hpy188I TCNGA 1 cut(s) 291
Hpy188III TCNNGA 3 cut(s) 14, 276, 440
Hpy8I GTNNAC 1 cut(s) 430
Hpy99I CGWCG 1 cut(s) 162
HpyAV CCTTC 1 cut(s) 314
HpyCH4III ACNGT 1 cut(s) 8
HpyCH4IV ACGT 2 cut(s) 205, 248
HpyCH4V TGCA 2 cut(s) 298, 392
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 45, 355
HpyF3I CTNAG 4 cut(s) 186, 216, 225, 419
HpySE526I ACGT 2 cut(s) 205, 248
Hsp92II CATG 2 cut(s) 63, 548
Kpn2I TCCGGA 1 cut(s) 439
Kzo9I GATC 6 cut(s) 22, 115, 122, 343, 353, 475
LguI GCTCTTC 1 cut(s) 135
LmnI GCTCC 1 cut(s) 35
LpnPI CCDG 9 cut(s) 27, 67, 161, 176, 228, 289, 390, 438, 453
MaeI CTAG 2 cut(s) 233, 491
MaeII ACGT 2 cut(s) 205, 248
MaeIII GTNAC 1 cut(s) 327
MalI GATC 6 cut(s) 24, 117, 124, 345, 355, 477
MboI GATC 6 cut(s) 22, 115, 122, 343, 353, 475
MboII GAAGA 2 cut(s) 122, 490
MfeI CAATTG 1 cut(s) 70
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 2 cut(s) 32, 379
MluCI AATT 1 cut(s) 70
MmeI TCCRAC 1 cut(s) 206
MroI TCCGGA 1 cut(s) 439
MseI TTAA 1 cut(s) 556
MslI CAYNNNNRTG 1 cut(s) 491
MspA1I CMGCKG 1 cut(s) 45
MspI CCGG 1 cut(s) 440
MunI CAATTG 1 cut(s) 70
Mva1269I GAATGC 1 cut(s) 300
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 3 cut(s) 36, 45, 355
NcoI CCATGG 1 cut(s) 544
NdeII GATC 6 cut(s) 22, 115, 122, 343, 353, 475
NlaIII CATG 2 cut(s) 63, 548
NlaIV GGNNCC 3 cut(s) 180, 345, 409
OliI CACNNNNGTG 1 cut(s) 491
PciSI GCTCTTC 1 cut(s) 135
PctI GAATGC 1 cut(s) 300
PfeI GAWTC 1 cut(s) 229
PkrI GCNGC 1 cut(s) 47
PsiI TTATAA 1 cut(s) 371
PspN4I GGNNCC 3 cut(s) 180, 345, 409
PspPI GGNCC 1 cut(s) 8
PsuI RGATCY 1 cut(s) 343
RsaI GTAC 1 cut(s) 531
RsaNI GTAC 1 cut(s) 530
RseI CAYNNNNRTG 1 cut(s) 491
SapI GCTCTTC 1 cut(s) 135
SaqAI TTAA 1 cut(s) 556
SatI GCNGC 1 cut(s) 46
Sau3AI GATC 6 cut(s) 22, 115, 122, 343, 353, 475
Sau96I GGNCC 1 cut(s) 8
SduI GDGCHC 2 cut(s) 32, 379
SetI ASST 9 cut(s) 130, 173, 208, 222, 243, 251, 271, 306, 553
SinI GGWCC 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 491
SpeI ACTAGT 1 cut(s) 490
Sse9I AATT 1 cut(s) 70
SsiI CCGC 4 cut(s) 45, 90, 319, 347
SspI AATATT 1 cut(s) 450
SspMI CTAG 2 cut(s) 233, 491
StyI CCWWGG 1 cut(s) 544
TaaI ACNGT 1 cut(s) 8
TaiI ACGT 2 cut(s) 208, 251
TasI AATT 1 cut(s) 70
TauI GCSGC 1 cut(s) 48
TfiI GAWTC 1 cut(s) 229
Tru1I TTAA 1 cut(s) 556
Tru9I TTAA 1 cut(s) 556
TscAI CASTG 2 cut(s) 32, 86
TspDTI ATGAA 1 cut(s) 183
TspGWI ACGGA 2 cut(s) 22, 135
TspRI CASTG 2 cut(s) 32, 86
VpaK11BI GGWCC 1 cut(s) 8
XspI CTAG 2 cut(s) 233, 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.