Rw7G032650

E3 ubiquitin-protein ligase RNF170-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007874
Physical Location & Seq
Reverse (-)
47683766 .. 47690127
6362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G032650.1

Sequence Viewer

Length: 558 bp
ATGGAGGCTCCTCCTGAAAACGATCTGTGCTCCGTCTGCCATGGCGTCTTCAACACTCCTTGTCAAGCCAATTGCTCTCACTGGTTTTGCGGAAATTGTATTATGCTTGTTTGGGATCATGGATCAGCACTTCAGCCTTGTAAATGCCCTCTCTGTCGTCGCCAGATAACCTTGCTGGTTCCAACTGAGGCTTCATTGAGCCAACGTGATATGCCTGAGGTGGCTGAGATTCTAGGCAGGGTTGAAACATATAATCGCTATTTTGGAGGTCAACCCAGAGGTCTTATTCAGAGAATGCAAGACCTTCCTTTTCTTTTGCGGAGGCTGTTACGAGAACTGTTGGATCCCCATAGATCGCTTCCCCTTGTTATAAGGGCTCGTGTCATTATTGCAATGATCCTAAGCGGTGCCTATGTACTCAGTCCTGTTGACTTTATTCCAGAAGCAATATTTGGTATAGTCGGTCTATTTGATGATCTCCTCATAGTACTCGTGGTCTTCCTCCACGTTGCTACCATTTATCGGTCGGTACTCTATCGACGCCATGGAGGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1232 zf-C3HC4 zf-C3HC4_3

Protein Analysis

185

Amino Acids

20.87

Weight (kDa)

7.59

Isoelectric Point (pI)

46.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1232 PF06803 129 - 164 3.9e-08 Protein of unknown function (DUF1232)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 371
AccB1I GGYRCC 1 cut(s) 407
AciI CCGC 3 cut(s) 90, 319, 405
AclWI GGATC 5 cut(s) 123, 130, 338, 351, 391
AcuI CTGAAG 1 cut(s) 116
AcyI GRCGYC 2 cut(s) 45, 541
AfaI GTAC 3 cut(s) 417, 489, 531
AfiI CCNNNNNNNGG 1 cut(s) 522
AgsI TTSAA 2 cut(s) 52, 245
AjuI GAANNNNNNNTTGG 4 cut(s) 175, 207, 435, 467
Alw21I GWGCWC 1 cut(s) 32
AlwI GGATC 5 cut(s) 123, 130, 338, 351, 391
AxyI CCTNAGG 1 cut(s) 216
BamHI GGATCC 1 cut(s) 343
BanI GGYRCC 1 cut(s) 407
BanII GRGCYC 1 cut(s) 379
BauI CACGAG 2 cut(s) 378, 491
BbsI GAAGAC 2 cut(s) 40, 490
Bbv12I GWGCWC 1 cut(s) 32
BfaI CTAG 1 cut(s) 233
BmcAI AGTACT 1 cut(s) 489
BmiI GGNNCC 4 cut(s) 9, 180, 345, 409
BpiI GAAGAC 2 cut(s) 40, 490
Bpu10I CCTNAGC 1 cut(s) 401
BsaHI GRCGYC 2 cut(s) 45, 541
BsaJI CCNNGG 2 cut(s) 40, 544
Bsc4I CCNNNNNNNGG 1 cut(s) 522
Bse1I ACTGG 1 cut(s) 86
Bse21I CCTNAGG 1 cut(s) 216
Bse3DI GCAATG 1 cut(s) 399
BseDI CCNNGG 2 cut(s) 40, 544
BseLI CCNNNNNNNGG 1 cut(s) 522
BseMI GCAATG 1 cut(s) 399
BseMII CTCAG 4 cut(s) 177, 207, 216, 433
BseNI ACTGG 1 cut(s) 86
BseRI GAGGAG 1 cut(s) 470
Bsh1285I CGRYCG 1 cut(s) 527
BshNI GGYRCC 1 cut(s) 407
BsiEI CGRYCG 1 cut(s) 527
BsiHKAI GWGCWC 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 522
BsmI GAATGC 1 cut(s) 300
Bsp1286I GDGCHC 2 cut(s) 32, 379
Bsp143I GATC 7 cut(s) 22, 115, 122, 343, 353, 396, 475
Bsp19I CCATGG 2 cut(s) 40, 544
BspACI CCGC 3 cut(s) 90, 319, 405
BspCNI CTCAG 4 cut(s) 178, 208, 217, 432
BspLI GGNNCC 4 cut(s) 9, 180, 345, 409
BspPI GGATC 5 cut(s) 123, 130, 338, 351, 391
BspT107I GGYRCC 1 cut(s) 407
BsrDI GCAATG 1 cut(s) 399
BsrI ACTGG 1 cut(s) 86
BssECI CCNNGG 2 cut(s) 40, 544
BssMI GATC 7 cut(s) 22, 115, 122, 343, 353, 396, 475
BssNI GRCGYC 2 cut(s) 45, 541
BssSI CACGAG 2 cut(s) 378, 491
BssT1I CCWWGG 2 cut(s) 40, 544
Bst2BI CACGAG 2 cut(s) 378, 491
Bst4CI ACNGT 1 cut(s) 339
BstACI GRCGYC 2 cut(s) 45, 541
BstDEI CTNAG 5 cut(s) 186, 216, 225, 401, 419
BstDSI CCRYGG 2 cut(s) 40, 544
BstKTI GATC 7 cut(s) 25, 118, 125, 346, 356, 399, 478
BstMBI GATC 7 cut(s) 22, 115, 122, 343, 353, 396, 475
BstMCI CGRYCG 1 cut(s) 527
BstMWI GCNNNNNNNGC 1 cut(s) 36
BstV2I GAAGAC 2 cut(s) 40, 490
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
Bsu36I CCTNAGG 1 cut(s) 216
BtgI CCRYGG 2 cut(s) 40, 544
BtsIMutI CAGTG 1 cut(s) 79
CseI GACGC 2 cut(s) 34, 549
Csp6I GTAC 3 cut(s) 416, 488, 530
CviAII CATG 3 cut(s) 41, 119, 545
CviJI RGCY 8 cut(s) 8, 68, 136, 191, 201, 224, 325, 377
CviKI_1 RGCY 8 cut(s) 8, 68, 136, 191, 201, 224, 325, 377
CviQI GTAC 3 cut(s) 416, 488, 530
DdeI CTNAG 5 cut(s) 186, 216, 225, 401, 419
DpnI GATC 7 cut(s) 24, 117, 124, 345, 355, 398, 477
DpnII GATC 7 cut(s) 22, 115, 122, 343, 353, 396, 475
Eco130I CCWWGG 2 cut(s) 40, 544
Eco24I GRGCYC 1 cut(s) 379
Eco57I CTGAAG 1 cut(s) 116
Eco81I CCTNAGG 1 cut(s) 216
EcoT14I CCWWGG 2 cut(s) 40, 544
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 2 cut(s) 40, 544
FaeI CATG 3 cut(s) 44, 122, 548
FatI CATG 3 cut(s) 40, 118, 544
FriOI GRGCYC 1 cut(s) 379
FspBI CTAG 1 cut(s) 233
HgaI GACGC 2 cut(s) 34, 549
Hin1I GRCGYC 2 cut(s) 45, 541
Hin1II CATG 3 cut(s) 44, 122, 548
HincII GTYRAC 2 cut(s) 272, 430
HindII GTYRAC 2 cut(s) 272, 430
HinfI GANTC 1 cut(s) 229
Hpy166II GTNNAC 2 cut(s) 272, 430
Hpy188I TCNGA 1 cut(s) 291
Hpy188III TCNNGA 2 cut(s) 14, 440
Hpy8I GTNNAC 2 cut(s) 272, 430
Hpy99I CGWCG 2 cut(s) 162, 543
HpyAV CCTTC 1 cut(s) 314
HpyCH4III ACNGT 1 cut(s) 339
HpyCH4IV ACGT 2 cut(s) 205, 507
HpyCH4V TGCA 2 cut(s) 298, 392
HpyF10VI GCNNNNNNNGC 1 cut(s) 36
HpyF3I CTNAG 5 cut(s) 186, 216, 225, 401, 419
HpySE526I ACGT 2 cut(s) 205, 507
Hsp92I GRCGYC 2 cut(s) 45, 541
Hsp92II CATG 3 cut(s) 44, 122, 548
Kzo9I GATC 7 cut(s) 22, 115, 122, 343, 353, 396, 475
LmnI GCTCC 2 cut(s) 13, 35
LpnPI CCDG 9 cut(s) 27, 67, 161, 176, 223, 228, 289, 438, 453
MaeI CTAG 1 cut(s) 233
MaeII ACGT 2 cut(s) 205, 507
MaeIII GTNAC 1 cut(s) 327
MalI GATC 7 cut(s) 24, 117, 124, 345, 355, 398, 477
MboI GATC 7 cut(s) 22, 115, 122, 343, 353, 396, 475
MboII GAAGA 2 cut(s) 40, 490
MfeI CAATTG 1 cut(s) 70
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 2 cut(s) 32, 379
MluCI AATT 2 cut(s) 70, 94
MmeI TCCRAC 2 cut(s) 206, 321
MseI TTAA 1 cut(s) 556
MunI CAATTG 1 cut(s) 70
Mva1269I GAATGC 1 cut(s) 300
MwoI GCNNNNNNNGC 1 cut(s) 36
NcoI CCATGG 2 cut(s) 40, 544
NdeII GATC 7 cut(s) 22, 115, 122, 343, 353, 396, 475
NlaIII CATG 3 cut(s) 44, 122, 548
NlaIV GGNNCC 4 cut(s) 9, 180, 345, 409
PctI GAATGC 1 cut(s) 300
PfeI GAWTC 1 cut(s) 229
PsiI TTATAA 1 cut(s) 371
PspN4I GGNNCC 4 cut(s) 9, 180, 345, 409
PsuI RGATCY 1 cut(s) 343
RsaI GTAC 3 cut(s) 417, 489, 531
RsaNI GTAC 3 cut(s) 416, 488, 530
SaqAI TTAA 1 cut(s) 556
Sau3AI GATC 7 cut(s) 22, 115, 122, 343, 353, 396, 475
ScaI AGTACT 1 cut(s) 489
SduI GDGCHC 2 cut(s) 32, 379
SetI ASST 8 cut(s) 173, 208, 222, 271, 283, 306, 510, 553
Sse9I AATT 2 cut(s) 70, 94
SsiI CCGC 3 cut(s) 90, 319, 405
SspI AATATT 1 cut(s) 450
SspMI CTAG 1 cut(s) 233
StyI CCWWGG 2 cut(s) 40, 544
TaaI ACNGT 1 cut(s) 339
TaiI ACGT 2 cut(s) 208, 510
TaqI TCGA 1 cut(s) 538
TaqII GACCGA 2 cut(s) 452, 513
TasI AATT 2 cut(s) 70, 94
TatI WGTACW 2 cut(s) 415, 487
TfiI GAWTC 1 cut(s) 229
Tru1I TTAA 1 cut(s) 556
Tru9I TTAA 1 cut(s) 556
TscAI CASTG 1 cut(s) 86
TspDTI ATGAA 1 cut(s) 183
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 1 cut(s) 86
XspI CTAG 1 cut(s) 233
ZrmI AGTACT 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.