Prupe.1G555800_v2.0.a1

E3 ubiquitin-protein ligase RNF170-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0007874
Physical Location & Seq
Forward (+)
45357647 .. 45363679
6033 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G555800.2

Sequence Viewer

Length: 504 bp
ATGGACGATCCTCCAGGCAGCGATATGTGCTCGGTCTGCCATGGCAACTTCGACACTCCTTGTCAAGCCAATTGTTCTCACTGGTTTTGCGAAATGGAGTGTGCTGTTTGGCATCATGGATCAGCACTTCAGCCTGCTTCATTAAGGCAGGCTGACAGACCAGAGGTGGCTGAGATTCTAGGCAAAGTTGAAAGGTACAATCGTTATTTTGGAGATCATCCCAGAAGTCTTATTCAGAGAGGGCAGGACCTTCCTTTTTTCCTGCGGAGGCTGCTGCGAGAACTAATTGATCCTAATAGATCTCTTCCTGCTCGTAGGATGGCTCAGATCTGTTTTGCAATGATATTGTTAGCAATCTACCTAGTGAGTCCTGTTGACCTTATTCCGGAAGCAATGTTTGGTTTACTTGGTTACGTTGATGATTTCATCGTGGCACTGGTCATCTTGTTTAATCTTGCTGCCATTTCTCGGTCGATACTCTATCACCGTCATGGAGGTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1232 zf-C3HC4 zf-C3HC4_3

Protein Analysis

168

Amino Acids

18.92

Weight (kDa)

6.13

Isoelectric Point (pI)

51.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 385
AciI CCGC 1 cut(s) 265
AclWI GGATC 3 cut(s) 2, 127, 284
AcuI CTGAAG 1 cut(s) 113
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 2 cut(s) 385, 468
AgsI TTSAA 1 cut(s) 191
AjnI CCWGG 1 cut(s) 13
AjuI GAANNNNNNNTTGG 2 cut(s) 381, 413
Alw21I GWGCWC 1 cut(s) 32
AlwI GGATC 3 cut(s) 2, 127, 284
Aor13HI TCCGGA 1 cut(s) 385
ApeKI GCWGC 4 cut(s) 18, 271, 274, 458
AspS9I GGNCC 1 cut(s) 247
AsuHPI GGTGA 1 cut(s) 476
AvaII GGWCC 1 cut(s) 247
BaeI ACNNNNGTAYC 2 cut(s) 187, 220
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 4 cut(s) 30, 258, 261, 445
BccI CCATC 1 cut(s) 313
BciT130I CCWGG 1 cut(s) 15
BfaI CTAG 2 cut(s) 179, 362
BglII AGATCT 2 cut(s) 299, 327
BisI GCNGC 4 cut(s) 19, 272, 275, 459
BlsI GCNGC 4 cut(s) 20, 273, 276, 460
Bme1390I CCNGG 1 cut(s) 15
Bme18I GGWCC 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 247
BmiI GGNNCC 1 cut(s) 499
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 1 cut(s) 121
BsaJI CCNNGG 1 cut(s) 40
BsaWI WCCGGW 1 cut(s) 385
Bsc4I CCNNNNNNNGG 2 cut(s) 385, 468
Bse1I ACTGG 2 cut(s) 86, 441
Bse3DI GCAATG 2 cut(s) 345, 399
BseAI TCCGGA 1 cut(s) 385
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 40
BseGI GGATG 2 cut(s) 217, 324
BseLI CCNNNNNNNGG 2 cut(s) 385, 468
BseMI GCAATG 2 cut(s) 345, 399
BseMII CTCAG 2 cut(s) 162, 338
BseNI ACTGG 2 cut(s) 86, 441
BseXI GCAGC 4 cut(s) 30, 258, 261, 445
Bsh1285I CGRYCG 1 cut(s) 473
BsiEI CGRYCG 1 cut(s) 473
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 1 cut(s) 386
BslI CCNNNNNNNGG 2 cut(s) 385, 468
Bsp1286I GDGCHC 1 cut(s) 32
Bsp13I TCCGGA 1 cut(s) 385
Bsp143I GATC 6 cut(s) 7, 119, 214, 289, 299, 327
Bsp19I CCATGG 1 cut(s) 40
BspACI CCGC 1 cut(s) 265
BspCNI CTCAG 2 cut(s) 163, 337
BspEI TCCGGA 1 cut(s) 385
BspLI GGNNCC 1 cut(s) 499
BspPI GGATC 3 cut(s) 2, 127, 284
BsrDI GCAATG 2 cut(s) 345, 399
BsrI ACTGG 2 cut(s) 86, 441
BssECI CCNNGG 1 cut(s) 40
BssMI GATC 6 cut(s) 7, 119, 214, 289, 299, 327
BssT1I CCWWGG 1 cut(s) 40
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 1 cut(s) 488
Bst6I CTCTTC 1 cut(s) 309
BstC8I GCNNGC 2 cut(s) 135, 150
BstDEI CTNAG 2 cut(s) 171, 324
BstDSI CCRYGG 1 cut(s) 40
BstF5I GGATG 2 cut(s) 217, 324
BstKTI GATC 6 cut(s) 10, 122, 217, 292, 302, 330
BstMBI GATC 6 cut(s) 7, 119, 214, 289, 299, 327
BstMCI CGRYCG 1 cut(s) 473
BstMWI GCNNNNNNNGC 3 cut(s) 27, 36, 271
BstNI CCWGG 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 13
BstV1I GCAGC 4 cut(s) 30, 258, 261, 445
BstX2I RGATCY 2 cut(s) 299, 327
BstYI RGATCY 2 cut(s) 299, 327
BtgI CCRYGG 1 cut(s) 40
BtsCI GGATG 2 cut(s) 217, 324
BtsIMutI CAGTG 2 cut(s) 79, 434
Cac8I GCNNGC 2 cut(s) 135, 150
Cfr13I GGNCC 1 cut(s) 247
Csp6I GTAC 1 cut(s) 196
CviAII CATG 3 cut(s) 41, 116, 491
CviJI RGCY 6 cut(s) 68, 133, 152, 170, 271, 323
CviKI_1 RGCY 6 cut(s) 68, 133, 152, 170, 271, 323
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 2 cut(s) 171, 324
DpnI GATC 6 cut(s) 9, 121, 216, 291, 301, 329
DpnII GATC 6 cut(s) 7, 119, 214, 289, 299, 327
Eam1104I CTCTTC 1 cut(s) 309
EarI CTCTTC 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 40
Eco47I GGWCC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 113
EcoO109I RGGNCCY 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 13
EcoT14I CCWWGG 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 3 cut(s) 44, 119, 494
FaiI YATR 4 cut(s) 26, 42, 117, 492
FatI CATG 3 cut(s) 40, 115, 490
Fnu4HI GCNGC 4 cut(s) 19, 272, 275, 459
FokI GGATG 2 cut(s) 204, 331
Fsp4HI GCNGC 4 cut(s) 19, 272, 275, 459
FspBI CTAG 2 cut(s) 179, 362
GluI GCNGC 4 cut(s) 19, 272, 275, 459
HapII CCGG 1 cut(s) 386
Hin1II CATG 3 cut(s) 44, 119, 494
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HinfI GANTC 2 cut(s) 175, 367
HpaII CCGG 1 cut(s) 386
HphI GGTGA 1 cut(s) 476
Hpy166II GTNNAC 2 cut(s) 376, 404
Hpy188I TCNGA 2 cut(s) 237, 327
Hpy188III TCNNGA 1 cut(s) 386
Hpy8I GTNNAC 2 cut(s) 376, 404
HpyAV CCTTC 1 cut(s) 260
HpyCH4III ACNGT 1 cut(s) 488
HpyCH4IV ACGT 1 cut(s) 414
HpyCH4V TGCA 1 cut(s) 338
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 36, 271
HpyF3I CTNAG 2 cut(s) 171, 324
HpySE526I ACGT 1 cut(s) 414
Hsp92II CATG 3 cut(s) 44, 119, 494
Kpn2I TCCGGA 1 cut(s) 385
Kzo9I GATC 6 cut(s) 7, 119, 214, 289, 299, 327
Lsp1109I GCAGC 4 cut(s) 30, 258, 261, 445
LweI GCATC 1 cut(s) 121
MaeI CTAG 2 cut(s) 179, 362
MaeII ACGT 1 cut(s) 414
MaeIII GTNAC 1 cut(s) 410
MalI GATC 6 cut(s) 9, 121, 216, 291, 301, 329
MboI GATC 6 cut(s) 7, 119, 214, 289, 299, 327
MboII GAAGA 1 cut(s) 296
MfeI CAATTG 1 cut(s) 70
MflI RGATCY 2 cut(s) 299, 327
MhlI GDGCHC 1 cut(s) 32
MluCI AATT 2 cut(s) 70, 285
MlyI GAGTC 1 cut(s) 376
MnlI CCTC 5 cut(s) 21, 157, 233, 261, 488
MroI TCCGGA 1 cut(s) 385
MseI TTAA 2 cut(s) 143, 450
MslI CAYNNNNRTG 1 cut(s) 489
MspI CCGG 1 cut(s) 386
MspR9I CCNGG 1 cut(s) 15
MunI CAATTG 1 cut(s) 70
MvaI CCWGG 1 cut(s) 15
MwoI GCNNNNNNNGC 3 cut(s) 27, 36, 271
NcoI CCATGG 1 cut(s) 40
NdeII GATC 6 cut(s) 7, 119, 214, 289, 299, 327
NlaIII CATG 3 cut(s) 44, 119, 494
NlaIV GGNNCC 1 cut(s) 499
PfeI GAWTC 1 cut(s) 175
PkrI GCNGC 4 cut(s) 20, 273, 276, 460
PleI GAGTC 1 cut(s) 375
PpsI GAGTC 1 cut(s) 375
PpuMI RGGWCCY 1 cut(s) 247
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 13
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 1 cut(s) 499
PspPI GGNCC 1 cut(s) 247
PspPPI RGGWCCY 1 cut(s) 247
PsuI RGATCY 2 cut(s) 299, 327
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 1 cut(s) 489
SaqAI TTAA 2 cut(s) 143, 450
SatI GCNGC 4 cut(s) 19, 272, 275, 459
Sau3AI GATC 6 cut(s) 7, 119, 214, 289, 299, 327
Sau96I GGNCC 1 cut(s) 247
SchI GAGTC 1 cut(s) 376
ScrFI CCNGG 1 cut(s) 15
SduI GDGCHC 1 cut(s) 32
SetI ASST 7 cut(s) 168, 197, 252, 363, 381, 417, 499
SfaNI GCATC 1 cut(s) 121
SinI GGWCC 1 cut(s) 247
SmiMI CAYNNNNRTG 1 cut(s) 489
Sse9I AATT 2 cut(s) 70, 285
SsiI CCGC 1 cut(s) 265
SspMI CTAG 2 cut(s) 179, 362
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 1 cut(s) 488
TaiI ACGT 1 cut(s) 417
TaqI TCGA 2 cut(s) 51, 473
TaqII GACCGA 2 cut(s) 22, 459
TasI AATT 2 cut(s) 70, 285
TfiI GAWTC 1 cut(s) 175
Tru1I TTAA 2 cut(s) 143, 450
Tru9I TTAA 2 cut(s) 143, 450
TscAI CASTG 2 cut(s) 86, 441
TseI GCWGC 4 cut(s) 18, 271, 274, 458
TspDTI ATGAA 2 cut(s) 129, 415
TspRI CASTG 2 cut(s) 86, 441
VpaK11BI GGWCC 1 cut(s) 247
XspI CTAG 2 cut(s) 179, 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.