MD08G1217700.v1.1

E3 ubiquitin-protein ligase RNF170-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0007874
Physical Location & Seq
Reverse (-)
28042909 .. 28046222
3314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1217700.v1.1.491

Sequence Viewer

Length: 558 bp
ATGGACGGCCCTCCAGGCGGCGATCTGTGTTCGGTGTGCCATGGCAACTTCGACACTCCTTGTCAGGCCAATTGCTCTCACTGGTTTTGCGGAAATTGTATTATGCTTGTTTGGGATCATGGATCAACAGTTCGCCCTTGTAAATGCCCGCTATGCCGTCGTCCCATCACTTTGTTGGTTCCAAGTAGGGCTTCTTCAAGGCAGCTTGATAGGCCAGAGGTTGCGGAGATTTTAGGCAAGATAGAAAGGTACAATCGTGTTTTTGGAGGTCATTCCAGAAGTCTTATTCAGAGAGGGCAGGACCTTCCTTTTCTCCTGCGGAGGCTGCTGCGAGAACTAATTAATCCTGATAGATCTCTTCCCCTTGTTATAAGGGCTCGTGTCTGGATTTCAATGATAATGGGTGCTGCGTACCTATTCAGCCCTTTTGACTTCATCCCGGAAGCAATATTTGGTATAGCTGGTCTCCTGGACGATTTCTTCATAGTGCTCATCGTCTTCTTCCACGTTGCTGCCATTTATCGGTCTGTACTCTATCGCCGCCATGGAGGTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1232 zf-C3HC4 zf-C3HC4_3

Protein Analysis

186

Amino Acids

20.9

Weight (kDa)

8.99

Isoelectric Point (pI)

47.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1232 PF06803 129 - 164 4e-09 Protein of unknown function (DUF1232)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 371
AciI CCGC 6 cut(s) 18, 90, 149, 224, 319, 541
AclWI GGATC 2 cut(s) 123, 130
AfaI GTAC 3 cut(s) 251, 413, 531
AfiI CCNNNNNNNGG 2 cut(s) 17, 522
AgsI TTSAA 2 cut(s) 198, 393
AjnI CCWGG 2 cut(s) 13, 468
AjuI GAANNNNNNNTTGG 4 cut(s) 175, 207, 435, 467
AloI GAACNNNNNNTCC 2 cut(s) 114, 146
AluBI AGCT 2 cut(s) 205, 461
AluI AGCT 2 cut(s) 205, 461
Alw21I GWGCWC 1 cut(s) 492
Alw26I GTCTC 1 cut(s) 470
AlwI GGATC 2 cut(s) 123, 130
AoxI GGCC 3 cut(s) 7, 66, 212
ApeKI GCWGC 5 cut(s) 202, 325, 328, 407, 512
AseI ATTAAT 1 cut(s) 342
AspS9I GGNCC 2 cut(s) 8, 301
AsuC2I CCSGG 1 cut(s) 440
AvaII GGWCC 1 cut(s) 301
BaeI ACNNNNGTAYC 2 cut(s) 241, 274
BanII GRGCYC 1 cut(s) 379
BarI GAAGNNNNNNTAC 2 cut(s) 178, 210
BauI CACGAG 1 cut(s) 378
BbsI GAAGAC 1 cut(s) 490
Bbv12I GWGCWC 1 cut(s) 492
BbvI GCAGC 5 cut(s) 214, 312, 315, 394, 499
BccI CCATC 1 cut(s) 173
BceAI ACGGC 2 cut(s) 22, 141
BciT130I CCWGG 2 cut(s) 15, 470
BcnI CCSGG 1 cut(s) 440
BcoDI GTCTC 1 cut(s) 470
BglI GCCNNNNNGGC 1 cut(s) 15
BglII AGATCT 1 cut(s) 353
BisI GCNGC 7 cut(s) 19, 203, 326, 329, 408, 513, 541
BlsI GCNGC 7 cut(s) 20, 204, 327, 330, 409, 514, 542
Bme1390I CCNGG 3 cut(s) 15, 440, 470
Bme18I GGWCC 1 cut(s) 301
BmgT120I GGNCC 2 cut(s) 8, 301
BmiI GGNNCC 2 cut(s) 180, 553
BmrFI CCNGG 3 cut(s) 15, 440, 470
BpiI GAAGAC 1 cut(s) 490
BpuMI CCSGG 1 cut(s) 440
BsaI GGTCTC 1 cut(s) 470
BsaJI CCNNGG 2 cut(s) 40, 544
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 522
Bse1I ACTGG 1 cut(s) 86
BseBI CCWGG 2 cut(s) 15, 470
BseDI CCNNGG 2 cut(s) 40, 544
BseGI GGATG 1 cut(s) 435
BseLI CCNNNNNNNGG 2 cut(s) 17, 522
BseNI ACTGG 1 cut(s) 86
BseXI GCAGC 5 cut(s) 214, 312, 315, 394, 499
BshFI GGCC 3 cut(s) 9, 68, 214
BsiHKAI GWGCWC 1 cut(s) 492
BsiSI CCGG 1 cut(s) 440
BslFI GGGAC 1 cut(s) 147
BslI CCNNNNNNNGG 2 cut(s) 17, 522
BsmAI GTCTC 1 cut(s) 470
BsmFI GGGAC 1 cut(s) 147
BsnI GGCC 3 cut(s) 9, 68, 214
Bso31I GGTCTC 1 cut(s) 470
Bsp1286I GDGCHC 2 cut(s) 379, 492
Bsp143I GATC 4 cut(s) 22, 115, 122, 353
Bsp19I CCATGG 2 cut(s) 40, 544
BspACI CCGC 6 cut(s) 18, 90, 149, 224, 319, 541
BspANI GGCC 3 cut(s) 9, 68, 214
BspLI GGNNCC 2 cut(s) 180, 553
BspPI GGATC 2 cut(s) 123, 130
BspTNI GGTCTC 1 cut(s) 470
BsrI ACTGG 1 cut(s) 86
BssECI CCNNGG 2 cut(s) 40, 544
BssMI GATC 4 cut(s) 22, 115, 122, 353
BssSI CACGAG 1 cut(s) 378
BssT1I CCWWGG 2 cut(s) 40, 544
Bst2BI CACGAG 1 cut(s) 378
Bst2UI CCWGG 2 cut(s) 15, 470
Bst4CI ACNGT 1 cut(s) 130
Bst6I CTCTTC 1 cut(s) 363
BstC8I GCNNGC 1 cut(s) 149
BstDSI CCRYGG 2 cut(s) 40, 544
BstF5I GGATG 1 cut(s) 435
BstKTI GATC 4 cut(s) 25, 118, 125, 356
BstMAI GTCTC 1 cut(s) 470
BstMBI GATC 4 cut(s) 22, 115, 122, 353
BstMWI GCNNNNNNNGC 4 cut(s) 15, 153, 211, 325
BstNI CCWGG 2 cut(s) 15, 470
BstSCI CCNGG 3 cut(s) 13, 438, 468
BstV1I GCAGC 5 cut(s) 214, 312, 315, 394, 499
BstV2I GAAGAC 1 cut(s) 490
BstX2I RGATCY 1 cut(s) 353
BstYI RGATCY 1 cut(s) 353
BsuRI GGCC 3 cut(s) 9, 68, 214
BtgI CCRYGG 2 cut(s) 40, 544
BtsCI GGATG 1 cut(s) 435
BtsIMutI CAGTG 1 cut(s) 79
Cac8I GCNNGC 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 8, 301
Csp6I GTAC 3 cut(s) 250, 412, 530
CviAII CATG 3 cut(s) 41, 119, 545
CviJI RGCY 9 cut(s) 9, 68, 191, 205, 214, 325, 377, 423, 461
CviKI_1 RGCY 9 cut(s) 9, 68, 191, 205, 214, 325, 377, 423, 461
CviQI GTAC 3 cut(s) 250, 412, 530
DpnI GATC 4 cut(s) 24, 117, 124, 355
DpnII GATC 4 cut(s) 22, 115, 122, 353
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
Eco130I CCWWGG 2 cut(s) 40, 544
Eco24I GRGCYC 1 cut(s) 379
Eco31I GGTCTC 1 cut(s) 470
Eco47I GGWCC 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 301
EcoRII CCWGG 2 cut(s) 13, 468
EcoT14I CCWWGG 2 cut(s) 40, 544
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 2 cut(s) 40, 544
FaeI CATG 3 cut(s) 44, 122, 548
FaiI YATR 8 cut(s) 42, 104, 120, 154, 371, 458, 485, 546
FalI AAGNNNNNCTT 2 cut(s) 175, 207
FaqI GGGAC 1 cut(s) 147
FatI CATG 3 cut(s) 40, 118, 544
FauI CCCGC 1 cut(s) 156
Fnu4HI GCNGC 7 cut(s) 19, 203, 326, 329, 408, 513, 541
FokI GGATG 1 cut(s) 422
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 7 cut(s) 19, 203, 326, 329, 408, 513, 541
GluI GCNGC 7 cut(s) 19, 203, 326, 329, 408, 513, 541
HaeIII GGCC 3 cut(s) 9, 68, 214
HapII CCGG 1 cut(s) 440
Hin1II CATG 3 cut(s) 44, 122, 548
HpaII CCGG 1 cut(s) 440
Hpy188I TCNGA 1 cut(s) 291
Hpy188III TCNNGA 4 cut(s) 276, 347, 385, 555
Hpy99I CGWCG 1 cut(s) 162
HpyAV CCTTC 1 cut(s) 314
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4IV ACGT 1 cut(s) 507
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 153, 211, 325
HpySE526I ACGT 1 cut(s) 507
Hsp92II CATG 3 cut(s) 44, 122, 548
Kzo9I GATC 4 cut(s) 22, 115, 122, 353
Lsp1109I GCAGC 5 cut(s) 214, 312, 315, 394, 499
MaeII ACGT 1 cut(s) 507
MalI GATC 4 cut(s) 24, 117, 124, 355
MboI GATC 4 cut(s) 22, 115, 122, 353
MboII GAAGA 5 cut(s) 186, 350, 472, 490, 493
MfeI CAATTG 1 cut(s) 70
MflI RGATCY 1 cut(s) 353
MhlI GDGCHC 2 cut(s) 379, 492
MluCI AATT 3 cut(s) 70, 94, 339
MnlI CCTC 6 cut(s) 21, 211, 260, 287, 315, 542
MseI TTAA 1 cut(s) 342
MspI CCGG 1 cut(s) 440
MspR9I CCNGG 3 cut(s) 15, 440, 470
MunI CAATTG 1 cut(s) 70
MvaI CCWGG 2 cut(s) 15, 470
MwoI GCNNNNNNNGC 4 cut(s) 15, 153, 211, 325
NciI CCSGG 1 cut(s) 440
NcoI CCATGG 2 cut(s) 40, 544
NdeII GATC 4 cut(s) 22, 115, 122, 353
NlaIII CATG 3 cut(s) 44, 122, 548
NlaIV GGNNCC 2 cut(s) 180, 553
PfoI TCCNGGA 2 cut(s) 438, 468
PkrI GCNGC 7 cut(s) 20, 204, 327, 330, 409, 514, 542
PpuMI RGGWCCY 1 cut(s) 301
PshBI ATTAAT 1 cut(s) 342
PsiI TTATAA 1 cut(s) 371
Psp5II RGGWCCY 1 cut(s) 301
Psp6I CCWGG 2 cut(s) 13, 468
PspGI CCWGG 2 cut(s) 13, 468
PspN4I GGNNCC 2 cut(s) 180, 553
PspPI GGNCC 2 cut(s) 8, 301
PspPPI RGGWCCY 1 cut(s) 301
PsuI RGATCY 1 cut(s) 353
RsaI GTAC 3 cut(s) 251, 413, 531
RsaNI GTAC 3 cut(s) 250, 412, 530
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 7 cut(s) 19, 203, 326, 329, 408, 513, 541
Sau3AI GATC 4 cut(s) 22, 115, 122, 353
Sau96I GGNCC 2 cut(s) 8, 301
ScrFI CCNGG 3 cut(s) 15, 440, 470
SduI GDGCHC 2 cut(s) 379, 492
SetI ASST 9 cut(s) 207, 222, 251, 271, 306, 417, 463, 510, 553
SinI GGWCC 1 cut(s) 301
Sse9I AATT 3 cut(s) 70, 94, 339
SsiI CCGC 6 cut(s) 18, 90, 149, 224, 319, 541
SspI AATATT 1 cut(s) 450
StyD4I CCNGG 3 cut(s) 13, 438, 468
StyI CCWWGG 2 cut(s) 40, 544
TaaI ACNGT 1 cut(s) 130
TaiI ACGT 1 cut(s) 510
TaqI TCGA 1 cut(s) 51
TaqII GACCGA 1 cut(s) 513
TasI AATT 3 cut(s) 70, 94, 339
TatI WGTACW 1 cut(s) 529
TauI GCSGC 2 cut(s) 21, 543
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 1 cut(s) 86
TseI GCWGC 5 cut(s) 202, 325, 328, 407, 512
TspDTI ATGAA 2 cut(s) 424, 472
TspRI CASTG 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 301
VspI ATTAAT 1 cut(s) 342
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.