AT1G22520

Domain of unknown function (DUF543)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010209
Physical Location & Seq
Reverse (-)
7952974 .. 7954546
1573 bp
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UTR
Exon/CDS
Intron
AT1G22520.1

Sequence Viewer

Length: 300 bp
ATGGAAACGACGAAGAGTAACAGCAGCGAGTCCGATGTCAACGCCAAATGGGACGCTTGTCTCGATCTCACTGCTCGTCGCTTTGTCTACTCTTCCCTCGGCGGCGCTTTCGCCGGTCTTCTCTTCTTCAGGAGTCCGGTTACGAGATGGGCGTCGATTGCTTTTGGTGCTGGAATTGGTATTGGTTCTGCATACACAGATTGTTCTCGTGTTTTTGATGCGTCTTCTTCAACTTCAGCTACTTTATTAGCAGCTCCCAAGAGTACGGAGACTTCTGTATCTCAGGCAGCAGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF543

Protein Analysis

99

Amino Acids

10.36

Weight (kDa)

4.99

Isoelectric Point (pI)

56.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF543 PF04418 4 - 73 3e-23 Domain of unknown function (DUF543)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 87
AciI CCGC 1 cut(s) 102
AcuI CTGAAG 2 cut(s) 112, 219
AcyI GRCGYC 1 cut(s) 152
AfaI GTAC 1 cut(s) 265
AgsI TTSAA 1 cut(s) 231
AluBI AGCT 2 cut(s) 239, 254
AluI AGCT 2 cut(s) 239, 254
Alw26I GTCTC 2 cut(s) 65, 263
ApeKI GCWGC 3 cut(s) 24, 251, 287
AspLEI GCGC 1 cut(s) 107
BaeI ACNNNNGTAYC 2 cut(s) 261, 294
BarI GAAGNNNNNNTAC 2 cut(s) 256, 288
BauI CACGAG 1 cut(s) 207
BbsI GAAGAC 2 cut(s) 110, 216
BbvI GCAGC 2 cut(s) 36, 263
BccI CCATC 1 cut(s) 141
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BcoDI GTCTC 2 cut(s) 65, 263
BfoI RGCGCY 1 cut(s) 108
BisI GCNGC 4 cut(s) 25, 103, 252, 288
BlsI GCNGC 4 cut(s) 26, 104, 253, 289
BmsI GCATC 1 cut(s) 208
BoxI GACNNNNGTC 1 cut(s) 57
BpiI GAAGAC 2 cut(s) 110, 216
BsaHI GRCGYC 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 97
BsaWI WCCGGW 1 cut(s) 136
Bse118I RCCGGY 1 cut(s) 113
BseDI CCNNGG 1 cut(s) 97
BseMII CTCAG 1 cut(s) 296
BseXI GCAGC 2 cut(s) 36, 263
BsiSI CCGG 2 cut(s) 114, 137
BslFI GGGAC 1 cut(s) 65
BsmAI GTCTC 2 cut(s) 65, 263
BsmFI GGGAC 1 cut(s) 65
Bsp143I GATC 1 cut(s) 64
BspACI CCGC 1 cut(s) 102
BspCNI CTCAG 1 cut(s) 295
BsrFI RCCGGY 1 cut(s) 113
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 1 cut(s) 97
BssMI GATC 1 cut(s) 64
BssNI GRCGYC 1 cut(s) 152
BssSI CACGAG 1 cut(s) 207
Bst2BI CACGAG 1 cut(s) 207
Bst6I CTCTTC 4 cut(s) 8, 97, 128, 288
BstACI GRCGYC 1 cut(s) 152
BstDEI CTNAG 1 cut(s) 282
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 1 cut(s) 67
BstMAI GTCTC 2 cut(s) 65, 263
BstMBI GATC 1 cut(s) 64
BstMWI GCNNNNNNNGC 2 cut(s) 158, 167
BstPAI GACNNNNGTC 1 cut(s) 57
BstV1I GCAGC 2 cut(s) 36, 263
BstV2I GAAGAC 2 cut(s) 110, 216
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 1 cut(s) 69
CfoI GCGC 1 cut(s) 107
Cfr10I RCCGGY 1 cut(s) 113
CseI GACGC 3 cut(s) 62, 141, 210
Csp6I GTAC 1 cut(s) 264
CviJI RGCY 2 cut(s) 239, 254
CviKI_1 RGCY 2 cut(s) 239, 254
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 1 cut(s) 282
DpnI GATC 1 cut(s) 66
DpnII GATC 1 cut(s) 64
Eam1104I CTCTTC 4 cut(s) 8, 97, 128, 288
EarI CTCTTC 4 cut(s) 8, 97, 128, 288
Eco57I CTGAAG 2 cut(s) 112, 219
FaiI YATR 1 cut(s) 193
FaqI GGGAC 1 cut(s) 65
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 4 cut(s) 25, 103, 252, 288
Fsp4HI GCNGC 4 cut(s) 25, 103, 252, 288
GlaI GCGC 1 cut(s) 106
GluI GCNGC 4 cut(s) 25, 103, 252, 288
HaeII RGCGCY 1 cut(s) 108
HapII CCGG 2 cut(s) 114, 137
HgaI GACGC 3 cut(s) 62, 141, 210
HhaI GCGC 1 cut(s) 107
Hin1I GRCGYC 1 cut(s) 152
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HinfI GANTC 2 cut(s) 29, 133
HpaII CCGG 2 cut(s) 114, 137
Hpy166II GTNNAC 2 cut(s) 40, 88
Hpy188I TCNGA 1 cut(s) 34
Hpy188III TCNNGA 2 cut(s) 62, 130
Hpy8I GTNNAC 2 cut(s) 40, 88
Hpy99I CGWCG 3 cut(s) 13, 81, 157
HpyCH4V TGCA 1 cut(s) 191
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 167
HpyF3I CTNAG 1 cut(s) 282
Hsp92I GRCGYC 1 cut(s) 152
HspAI GCGC 1 cut(s) 105
Kzo9I GATC 1 cut(s) 64
LmnI GCTCC 1 cut(s) 259
LpnPI CCDG 5 cut(s) 115, 127, 150, 156, 269
Lsp1109I GCAGC 2 cut(s) 36, 263
LweI GCATC 1 cut(s) 208
MaeIII GTNAC 2 cut(s) 17, 139
MalI GATC 1 cut(s) 66
MboI GATC 1 cut(s) 64
MboII GAAGA 7 cut(s) 25, 84, 110, 115, 118, 216, 219
MluCI AATT 1 cut(s) 174
MlyI GAGTC 2 cut(s) 38, 142
MnlI CCTC 1 cut(s) 107
MspI CCGG 2 cut(s) 114, 137
MwoI GCNNNNNNNGC 2 cut(s) 158, 167
NdeII GATC 1 cut(s) 64
NmeAIII GCCGAG 1 cut(s) 78
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 149
PkrI GCNGC 4 cut(s) 26, 104, 253, 289
PleI GAGTC 2 cut(s) 37, 141
PpsI GAGTC 2 cut(s) 37, 141
PshAI GACNNNNGTC 1 cut(s) 57
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
SatI GCNGC 4 cut(s) 25, 103, 252, 288
Sau3AI GATC 1 cut(s) 64
SchI GAGTC 2 cut(s) 38, 142
SetI ASST 2 cut(s) 241, 256
SfaNI GCATC 1 cut(s) 208
Sse9I AATT 1 cut(s) 174
SsiI CCGC 1 cut(s) 102
TaqI TCGA 2 cut(s) 63, 155
TasI AATT 1 cut(s) 174
TauI GCSGC 1 cut(s) 105
TscAI CASTG 1 cut(s) 76
TseI GCWGC 3 cut(s) 24, 251, 287
TspGWI ACGGA 1 cut(s) 281
TspRI CASTG 1 cut(s) 76
XmiI GTMKAC 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.