Rorug07G0241700

Domain of unknown function (DUF543)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0010209
Physical Location & Seq
Forward (+)
22232288 .. 22233837
1550 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0241700.1

Sequence Viewer

Length: 738 bp
ATGGTGGTTTTCTTCAAAGGAGGAGTTAAGCAACGACTGGGAAGATCGATTAGTTTGAAGACAAAGCGAGCATTGCTAGACGATCTCCTGTTGCAGGAGTCACCAGGTTCGGCAAATACAGCTCGACATGTGATCATCGTCATGGATGCCATGAAAGAGTTCAACACGGCGCCTCTCGAGTGGGCACTGGAGCATGTAATCAAGGCTGGCTCTGTTGTTATTCTTCTCGGTGTGATGCCATGGCTCAACATTCCTCTGTCCTCCAAGACATGGCTGGATATTTGGCCAGTTGGGTTAGAGGAACTATCAGCCGTGAAAGAGAAAATTGAGTGGAAGAGCGATGTCAAATATCTGAAGTTGCAGGCAGTTGTGGATCTTTGCAAAAACTATGGGGTTGTACTACAGAAGAAGGTTATAATGGGTTATCCCTCAAGACTGCTTGTTGTTGAAGAAATTATAAGCTTTCATGCCAAATGGGTCGTCTTCGACAGACATCAAAAGAATGACAGGAAGTTTTACAATGGGAAACTCCCATGCAACATGGTGATGATGAACGAAGAAGGGGAGGCTGACATGATCAAAGGGAGGACTATGATAGATAGCGACGTCGGAGGCTCAACACCTTCTCTGTTATCTACTCCGATTCTCATGATCTCTGATCATTTGAAGGAATTTCTTAACAAACCGAAACGTGCTCGAGAAGACCGCTCTAGATCCAGATCCCAAGGCGATGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF543

Protein Analysis

245

Amino Acids

27.76

Weight (kDa)

9.32

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 416, 458
AatII GACGTC 1 cut(s) 609
AccB1I GGYRCC 1 cut(s) 169
AccB7I CCANNNNNTGG 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 708
AciI CCGC 1 cut(s) 706
AclWI GGATC 3 cut(s) 381, 708, 714
AcoI YGGCCR 1 cut(s) 284
AcsI RAATTY 1 cut(s) 671
AcuI CTGAAG 1 cut(s) 374
AcyI GRCGYC 2 cut(s) 170, 606
AfaI GTAC 1 cut(s) 399
AfiI CCNNNNNNNGG 2 cut(s) 94, 270
AflIII ACRYGT 1 cut(s) 127
AgsI TTSAA 5 cut(s) 16, 58, 163, 449, 667
AjnI CCWGG 1 cut(s) 103
AluBI AGCT 2 cut(s) 122, 462
AluI AGCT 2 cut(s) 122, 462
Alw21I GWGCWC 1 cut(s) 697
AlwI GGATC 3 cut(s) 381, 708, 714
Ama87I CYCGRG 2 cut(s) 176, 696
AoxI GGCC 1 cut(s) 284
ApoI RAATTY 1 cut(s) 671
ArsI GACNNNNNNTTYG 2 cut(s) 465, 497
Asp700I GAANNNNTTC 1 cut(s) 158
AspLEI GCGC 1 cut(s) 172
AsuHPI GGTGA 2 cut(s) 93, 556
AvaI CYCGRG 2 cut(s) 176, 696
BaeGI GKGCMC 1 cut(s) 187
BalI TGGCCA 1 cut(s) 286
BanI GGYRCC 1 cut(s) 169
BbsI GAAGAC 3 cut(s) 65, 475, 708
Bbv12I GWGCWC 1 cut(s) 697
BceAI ACGGC 2 cut(s) 183, 296
BciT130I CCWGG 1 cut(s) 105
BclI TGATCA 3 cut(s) 132, 576, 658
BfaI CTAG 3 cut(s) 77, 711, 736
BfmI CTRYAG 1 cut(s) 401
BfoI RGCGCY 1 cut(s) 173
Bme1390I CCNGG 1 cut(s) 105
BmeT110I CYCGRG 2 cut(s) 176, 696
BmiI GGNNCC 1 cut(s) 171
BmrFI CCNGG 1 cut(s) 105
BmrI ACTGGG 1 cut(s) 47
BmsI GCATC 2 cut(s) 136, 225
BmuI ACTGGG 1 cut(s) 47
BpiI GAAGAC 3 cut(s) 65, 475, 708
BpmI CTGGAG 1 cut(s) 209
BpuEI CTTGAG 1 cut(s) 415
Bsa29I ATCGAT 1 cut(s) 47
BsaBI GATNNNNATC 1 cut(s) 718
BsaHI GRCGYC 2 cut(s) 170, 606
BsaJI CCNNGG 2 cut(s) 239, 724
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 270
Bse1I ACTGG 3 cut(s) 42, 192, 287
Bse3DI GCAATG 1 cut(s) 71
Bse8I GATNNNNATC 1 cut(s) 718
BseBI CCWGG 1 cut(s) 105
BseCI ATCGAT 1 cut(s) 47
BseDI CCNNGG 2 cut(s) 239, 724
BseGI GGATG 1 cut(s) 151
BseJI GATNNNNATC 1 cut(s) 718
BseLI CCNNNNNNNGG 2 cut(s) 94, 270
BseMI GCAATG 1 cut(s) 71
BseNI ACTGG 3 cut(s) 42, 192, 287
BseRI GAGGAG 1 cut(s) 36
BseSI GKGCMC 1 cut(s) 187
BshFI GGCC 1 cut(s) 286
BshNI GGYRCC 1 cut(s) 169
BshVI ATCGAT 1 cut(s) 47
BsiHKAI GWGCWC 1 cut(s) 697
BsiHKCI CYCGRG 2 cut(s) 176, 696
BslI CCNNNNNNNGG 2 cut(s) 94, 270
BsnI GGCC 1 cut(s) 286
BsoBI CYCGRG 2 cut(s) 176, 696
Bsp1286I GDGCHC 2 cut(s) 187, 697
Bsp143I GATC 9 cut(s) 44, 82, 132, 373, 576, 651, 658, 713, 719
Bsp19I CCATGG 1 cut(s) 239
BspACI CCGC 1 cut(s) 706
BspANI GGCC 1 cut(s) 286
BspDI ATCGAT 1 cut(s) 47
BspHI TCATGA 1 cut(s) 648
BspLI GGNNCC 1 cut(s) 171
BspPI GGATC 3 cut(s) 381, 708, 714
BspQI GCTCTTC 1 cut(s) 329
BspT107I GGYRCC 1 cut(s) 169
BsrBI CCGCTC 1 cut(s) 708
BsrDI GCAATG 1 cut(s) 71
BsrI ACTGG 3 cut(s) 42, 192, 287
BssECI CCNNGG 2 cut(s) 239, 724
BssMI GATC 9 cut(s) 44, 82, 132, 373, 576, 651, 658, 713, 719
BssNI GRCGYC 2 cut(s) 170, 606
BssT1I CCWWGG 2 cut(s) 239, 724
Bst2UI CCWGG 1 cut(s) 105
Bst6I CTCTTC 1 cut(s) 329
BstACI GRCGYC 2 cut(s) 170, 606
BstC8I GCNNGC 3 cut(s) 69, 208, 363
BstDSI CCRYGG 1 cut(s) 239
BstENI CCTNNNNNAGG 1 cut(s) 92
BstF5I GGATG 1 cut(s) 151
BstH2I RGCGCY 1 cut(s) 173
BstHHI GCGC 1 cut(s) 172
BstKTI GATC 9 cut(s) 47, 85, 135, 376, 579, 654, 661, 716, 722
BstMBI GATC 9 cut(s) 44, 82, 132, 373, 576, 651, 658, 713, 719
BstMWI GCNNNNNNNGC 2 cut(s) 73, 119
BstNI CCWGG 1 cut(s) 105
BstNSI RCATGY 2 cut(s) 131, 197
BstSCI CCNGG 1 cut(s) 103
BstSFI CTRYAG 1 cut(s) 401
BstSLI GKGCMC 1 cut(s) 187
BstV2I GAAGAC 3 cut(s) 65, 475, 708
BstX2I RGATCY 3 cut(s) 373, 713, 719
BstYI RGATCY 3 cut(s) 373, 713, 719
Bsu15I ATCGAT 1 cut(s) 47
BsuRI GGCC 1 cut(s) 286
BsuTUI ATCGAT 1 cut(s) 47
BtgI CCRYGG 1 cut(s) 239
BtgZI GCGATG 1 cut(s) 354
BtsCI GGATG 1 cut(s) 151
BtsIMutI CAGTG 1 cut(s) 185
Cac8I GCNNGC 3 cut(s) 69, 208, 363
CciI TCATGA 1 cut(s) 648
CfoI GCGC 1 cut(s) 172
ClaI ATCGAT 1 cut(s) 47
CsiI ACCWGGT 1 cut(s) 103
Csp6I GTAC 1 cut(s) 398
CviQI GTAC 1 cut(s) 398
DinI GGCGCC 1 cut(s) 171
DpnI GATC 9 cut(s) 46, 84, 134, 375, 578, 653, 660, 715, 721
DpnII GATC 9 cut(s) 44, 82, 132, 373, 576, 651, 658, 713, 719
EaeI YGGCCR 1 cut(s) 284
Eam1104I CTCTTC 1 cut(s) 329
EarI CTCTTC 1 cut(s) 329
Eco130I CCWWGG 2 cut(s) 239, 724
Eco57I CTGAAG 1 cut(s) 374
Eco88I CYCGRG 2 cut(s) 176, 696
EcoNI CCTNNNNNAGG 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 103
EcoT14I CCWWGG 2 cut(s) 239, 724
EgeI GGCGCC 1 cut(s) 171
EheI GGCGCC 1 cut(s) 171
ErhI CCWWGG 2 cut(s) 239, 724
FbaI TGATCA 3 cut(s) 132, 576, 658
FokI GGATG 1 cut(s) 158
FspBI CTAG 3 cut(s) 77, 711, 736
GlaI GCGC 1 cut(s) 171
GsuI CTGGAG 1 cut(s) 209
HaeII RGCGCY 1 cut(s) 173
HaeIII GGCC 1 cut(s) 286
HhaI GCGC 1 cut(s) 172
Hin1I GRCGYC 2 cut(s) 170, 606
Hin6I GCGC 1 cut(s) 170
HinP1I GCGC 1 cut(s) 170
HindIII AAGCTT 1 cut(s) 460
HinfI GANTC 2 cut(s) 98, 643
HphI GGTGA 2 cut(s) 93, 556
Hpy188I TCNGA 4 cut(s) 354, 611, 642, 658
Hpy188III TCNNGA 6 cut(s) 176, 432, 649, 698, 711, 717
Hpy99I CGWCG 2 cut(s) 608, 611
HpyAV CCTTC 4 cut(s) 403, 554, 633, 661
HpyCH4IV ACGT 2 cut(s) 606, 691
HpyCH4V TGCA 4 cut(s) 94, 361, 381, 537
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 119
HpySE526I ACGT 2 cut(s) 606, 691
Hsp92I GRCGYC 2 cut(s) 170, 606
HspAI GCGC 1 cut(s) 170
KasI GGCGCC 1 cut(s) 169
Ksp22I TGATCA 3 cut(s) 132, 576, 658
Kzo9I GATC 9 cut(s) 44, 82, 132, 373, 576, 651, 658, 713, 719
LguI GCTCTTC 1 cut(s) 329
LmnI GCTCC 1 cut(s) 190
LweI GCATC 2 cut(s) 136, 225
MabI ACCWGGT 1 cut(s) 103
MaeI CTAG 3 cut(s) 77, 711, 736
MaeII ACGT 2 cut(s) 606, 691
MaeIII GTNAC 1 cut(s) 99
MalI GATC 9 cut(s) 46, 84, 134, 375, 578, 653, 660, 715, 721
MbiI CCGCTC 1 cut(s) 708
MboI GATC 9 cut(s) 44, 82, 132, 373, 576, 651, 658, 713, 719
MflI RGATCY 3 cut(s) 373, 713, 719
MhlI GDGCHC 2 cut(s) 187, 697
MlsI TGGCCA 1 cut(s) 286
MluCI AATT 3 cut(s) 324, 453, 671
MluNI TGGCCA 1 cut(s) 286
Mly113I GGCGCC 1 cut(s) 170
MlyI GAGTC 1 cut(s) 107
MmeI TCCRAC 1 cut(s) 589
MnlI CCTC 9 cut(s) 14, 183, 264, 271, 292, 439, 559, 579, 605
Mox20I TGGCCA 1 cut(s) 286
MroXI GAANNNNTTC 1 cut(s) 158
MscI TGGCCA 1 cut(s) 286
MseI TTAA 2 cut(s) 27, 678
MslI CAYNNNNRTG 2 cut(s) 140, 545
Msp20I TGGCCA 1 cut(s) 286
MspR9I CCNGG 1 cut(s) 105
MvaI CCWGG 1 cut(s) 105
MwoI GCNNNNNNNGC 2 cut(s) 73, 119
NarI GGCGCC 1 cut(s) 170
NcoI CCATGG 1 cut(s) 239
NdeII GATC 9 cut(s) 44, 82, 132, 373, 576, 651, 658, 713, 719
NlaIV GGNNCC 1 cut(s) 171
NmuCI GTSAC 1 cut(s) 99
NspI RCATGY 2 cut(s) 131, 197
PaeR7I CTCGAG 2 cut(s) 176, 696
PagI TCATGA 1 cut(s) 648
PciI ACATGT 1 cut(s) 127
PciSI GCTCTTC 1 cut(s) 329
PdmI GAANNNNTTC 1 cut(s) 158
PfeI GAWTC 1 cut(s) 643
PflMI CCANNNNNTGG 1 cut(s) 270
PleI GAGTC 1 cut(s) 106
PluTI GGCGCC 1 cut(s) 173
PpsI GAGTC 1 cut(s) 106
PscI ACATGT 1 cut(s) 127
PsiI TTATAA 2 cut(s) 416, 458
Psp6I CCWGG 1 cut(s) 103
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 1 cut(s) 171
PsuI RGATCY 3 cut(s) 373, 713, 719
RsaI GTAC 1 cut(s) 399
RsaNI GTAC 1 cut(s) 398
RseI CAYNNNNRTG 2 cut(s) 140, 545
SapI GCTCTTC 1 cut(s) 329
SaqAI TTAA 2 cut(s) 27, 678
Sau3AI GATC 9 cut(s) 44, 82, 132, 373, 576, 651, 658, 713, 719
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 2 cut(s) 187, 697
SetI ASST 7 cut(s) 109, 124, 414, 464, 609, 625, 694
SexAI ACCWGGT 1 cut(s) 103
SfaNI GCATC 2 cut(s) 136, 225
SfcI CTRYAG 1 cut(s) 401
SfoI GGCGCC 1 cut(s) 171
Sfr274I CTCGAG 2 cut(s) 176, 696
SlaI CTCGAG 2 cut(s) 176, 696
SmiMI CAYNNNNRTG 2 cut(s) 140, 545
SmlI CTYRAG 3 cut(s) 176, 430, 696
SmoI CTYRAG 3 cut(s) 176, 430, 696
Sse9I AATT 3 cut(s) 324, 453, 671
SsiI CCGC 1 cut(s) 706
SspDI GGCGCC 1 cut(s) 169
SspMI CTAG 3 cut(s) 77, 711, 736
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 2 cut(s) 239, 724
TaiI ACGT 2 cut(s) 609, 694
TaqI TCGA 5 cut(s) 47, 124, 177, 486, 697
TasI AATT 3 cut(s) 324, 453, 671
TatI WGTACW 1 cut(s) 397
TfiI GAWTC 1 cut(s) 643
Tru1I TTAA 2 cut(s) 27, 678
Tru9I TTAA 2 cut(s) 27, 678
TscAI CASTG 1 cut(s) 192
TseFI GTSAC 1 cut(s) 99
Tsp45I GTSAC 1 cut(s) 99
TspDTI ATGAA 3 cut(s) 167, 455, 566
TspRI CASTG 1 cut(s) 192
Van91I CCANNNNNTGG 1 cut(s) 270
XagI CCTNNNNNAGG 1 cut(s) 92
XapI RAATTY 1 cut(s) 671
XbaI TCTAGA 1 cut(s) 710
XceI RCATGY 2 cut(s) 131, 197
XcmI CCANNNNNNNNNTGG 1 cut(s) 271
XhoI CTCGAG 2 cut(s) 176, 696
XmnI GAANNNNTTC 1 cut(s) 158
XspI CTAG 3 cut(s) 77, 711, 736
ZraI GACGTC 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.