MD15G1405800.v1.1

Domain of unknown function (DUF543)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010209
Physical Location & Seq
Reverse (-)
50650499 .. 50654388
3890 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1405800.v1.1.491

Sequence Viewer

Length: 291 bp
ATGACAGACAGCAAGGAAATCATCCCACCGCCGTACGACCTGAACGCCAAATGGGACGCATGTCTCGATCTGACGGTCCGGCGCTTCGTCTACTCCTCCTTGGGCGGTGCCTTTGGCGGCCTCCTTCTGTTCAGGAGTCCTGTGACTCGTTGGGCTTCTGTAGCTTTTGGCGCTGGAGTGGGCATTGGATGTGCATACACAGAGTGCTCCCGTTTGTTTGAGGGATCTCCTGCAAAGTTGGCTGCTCCTAAGATTATTGAGGTCCCTGCTCCTCAGGACGGACACGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF543

Protein Analysis

97

Amino Acids

10.26

Weight (kDa)

5.71

Isoelectric Point (pI)

76.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF543 PF04418 5 - 73 1.2e-22 Domain of unknown function (DUF543)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 107
AccI GTMKAC 1 cut(s) 90
AciI CCGC 3 cut(s) 29, 105, 117
AclWI GGATC 1 cut(s) 232
AdeI CACNNNGTG 1 cut(s) 204
AfaI GTAC 1 cut(s) 35
AfiI CCNNNNNNNGG 1 cut(s) 278
AluBI AGCT 1 cut(s) 164
AluI AGCT 1 cut(s) 164
Alw21I GWGCWC 1 cut(s) 209
Alw26I GTCTC 1 cut(s) 68
AlwI GGATC 1 cut(s) 232
AoxI GGCC 1 cut(s) 118
ApeKI GCWGC 1 cut(s) 242
AspLEI GCGC 2 cut(s) 84, 173
AspS9I GGNCC 2 cut(s) 76, 262
AvaII GGWCC 2 cut(s) 76, 262
AxyI CCTNAGG 1 cut(s) 273
BanI GGYRCC 1 cut(s) 107
Bbv12I GWGCWC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 229
BceAI ACGGC 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 68
BfmI CTRYAG 1 cut(s) 159
BfoI RGCGCY 2 cut(s) 85, 174
BisI GCNGC 2 cut(s) 118, 243
BlsI GCNGC 2 cut(s) 119, 244
Bme18I GGWCC 2 cut(s) 76, 262
BmgT120I GGNCC 2 cut(s) 76, 262
BmiI GGNNCC 2 cut(s) 109, 264
BoxI GACNNNNGTC 1 cut(s) 60
BpmI CTGGAG 1 cut(s) 195
BsaJI CCNNGG 1 cut(s) 99
Bsc4I CCNNNNNNNGG 1 cut(s) 278
Bse21I CCTNAGG 1 cut(s) 273
BseDI CCNNGG 1 cut(s) 99
BseGI GGATG 2 cut(s) 21, 194
BseLI CCNNNNNNNGG 1 cut(s) 278
BseMII CTCAG 1 cut(s) 287
BseRI GAGGAG 2 cut(s) 85, 261
BseXI GCAGC 1 cut(s) 229
BshFI GGCC 1 cut(s) 120
BshNI GGYRCC 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 209
BsiSI CCGG 1 cut(s) 79
BsiWI CGTACG 1 cut(s) 33
BslFI GGGAC 2 cut(s) 68, 248
BslI CCNNNNNNNGG 1 cut(s) 278
BsmAI GTCTC 1 cut(s) 68
BsmFI GGGAC 2 cut(s) 68, 248
BsnI GGCC 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 209
Bsp143I GATC 2 cut(s) 67, 224
BspACI CCGC 3 cut(s) 29, 105, 117
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 1 cut(s) 286
BspLI GGNNCC 2 cut(s) 109, 264
BspPI GGATC 1 cut(s) 232
BspT107I GGYRCC 1 cut(s) 107
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 2 cut(s) 67, 224
BssT1I CCWWGG 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 76
BstDEI CTNAG 2 cut(s) 249, 273
BstF5I GGATG 2 cut(s) 21, 194
BstH2I RGCGCY 2 cut(s) 85, 174
BstHHI GCGC 2 cut(s) 84, 173
BstKTI GATC 2 cut(s) 70, 227
BstMAI GTCTC 1 cut(s) 68
BstMBI GATC 2 cut(s) 67, 224
BstMWI GCNNNNNNNGC 3 cut(s) 161, 170, 239
BstNSI RCATGY 1 cut(s) 63
BstPAI GACNNNNGTC 1 cut(s) 60
BstSFI CTRYAG 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 229
BstX2I RGATCY 1 cut(s) 224
BstYI RGATCY 1 cut(s) 224
Bsu36I CCTNAGG 1 cut(s) 273
BsuRI GGCC 1 cut(s) 120
BtsCI GGATG 2 cut(s) 21, 194
CfoI GCGC 2 cut(s) 84, 173
Cfr13I GGNCC 2 cut(s) 76, 262
CpoI CGGWCCG 1 cut(s) 76
CseI GACGC 1 cut(s) 65
Csp6I GTAC 1 cut(s) 34
CspI CGGWCCG 1 cut(s) 76
CviAII CATG 1 cut(s) 60
CviJI RGCY 4 cut(s) 120, 155, 164, 242
CviKI_1 RGCY 4 cut(s) 120, 155, 164, 242
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 2 cut(s) 249, 273
DpnI GATC 2 cut(s) 69, 226
DpnII GATC 2 cut(s) 67, 224
DraIII CACNNNGTG 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 99
Eco47I GGWCC 2 cut(s) 76, 262
Eco81I CCTNAGG 1 cut(s) 273
EcoO109I RGGNCCY 1 cut(s) 262
EcoT14I CCWWGG 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 1 cut(s) 63
FaiI YATR 2 cut(s) 61, 196
FaqI GGGAC 2 cut(s) 68, 248
FatI CATG 1 cut(s) 59
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 2 cut(s) 118, 243
FokI GGATG 2 cut(s) 8, 201
Fsp4HI GCNGC 2 cut(s) 118, 243
GlaI GCGC 2 cut(s) 83, 172
GluI GCNGC 2 cut(s) 118, 243
GsuI CTGGAG 1 cut(s) 195
HaeII RGCGCY 2 cut(s) 85, 174
HaeIII GGCC 1 cut(s) 120
HapII CCGG 1 cut(s) 79
HgaI GACGC 1 cut(s) 65
HhaI GCGC 2 cut(s) 84, 173
Hin1II CATG 1 cut(s) 63
Hin6I GCGC 2 cut(s) 82, 171
HinP1I GCGC 2 cut(s) 82, 171
HinfI GANTC 2 cut(s) 136, 145
HpaII CCGG 1 cut(s) 79
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 1 cut(s) 72
Hpy188III TCNNGA 3 cut(s) 65, 133, 275
Hpy8I GTNNAC 1 cut(s) 91
HpyAV CCTTC 1 cut(s) 134
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4V TGCA 2 cut(s) 194, 233
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 170, 239
HpyF3I CTNAG 2 cut(s) 249, 273
Hsp92II CATG 1 cut(s) 63
HspAI GCGC 2 cut(s) 82, 171
Kzo9I GATC 2 cut(s) 67, 224
LmnI GCTCC 3 cut(s) 212, 250, 274
LpnPI CCDG 8 cut(s) 53, 92, 118, 153, 159, 243, 260, 279
Lsp1109I GCAGC 1 cut(s) 229
MaeIII GTNAC 1 cut(s) 142
MalI GATC 2 cut(s) 69, 226
MboI GATC 2 cut(s) 67, 224
MflI RGATCY 1 cut(s) 224
MhlI GDGCHC 1 cut(s) 209
MlyI GAGTC 2 cut(s) 139, 145
MnlI CCTC 5 cut(s) 106, 131, 214, 253, 282
MspI CCGG 1 cut(s) 79
MwoI GCNNNNNNNGC 3 cut(s) 161, 170, 239
NdeII GATC 2 cut(s) 67, 224
NlaIII CATG 1 cut(s) 63
NlaIV GGNNCC 2 cut(s) 109, 264
NmuCI GTSAC 1 cut(s) 142
NspI RCATGY 1 cut(s) 63
PcsI WCGNNNNNNNCGW 1 cut(s) 63
Pfl23II CGTACG 1 cut(s) 33
PkrI GCNGC 2 cut(s) 119, 244
PleI GAGTC 2 cut(s) 139, 144
PpsI GAGTC 2 cut(s) 139, 144
PpuMI RGGWCCY 1 cut(s) 262
PshAI GACNNNNGTC 1 cut(s) 60
Psp5II RGGWCCY 1 cut(s) 262
PspLI CGTACG 1 cut(s) 33
PspN4I GGNNCC 2 cut(s) 109, 264
PspPI GGNCC 2 cut(s) 76, 262
PspPPI RGGWCCY 1 cut(s) 262
PsuI RGATCY 1 cut(s) 224
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
Rsr2I CGGWCCG 1 cut(s) 76
RsrII CGGWCCG 1 cut(s) 76
SatI GCNGC 2 cut(s) 118, 243
Sau3AI GATC 2 cut(s) 67, 224
Sau96I GGNCC 2 cut(s) 76, 262
SchI GAGTC 2 cut(s) 139, 145
SduI GDGCHC 1 cut(s) 209
SetI ASST 3 cut(s) 42, 166, 264
SfcI CTRYAG 1 cut(s) 159
SinI GGWCC 2 cut(s) 76, 262
SsiI CCGC 3 cut(s) 29, 105, 117
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 1 cut(s) 76
TaqI TCGA 1 cut(s) 66
TauI GCSGC 1 cut(s) 120
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 1 cut(s) 242
Tsp45I GTSAC 1 cut(s) 142
VpaK11BI GGWCC 2 cut(s) 76, 262
XceI RCATGY 1 cut(s) 63
XmiI GTMKAC 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.