AT1G72170

Domain of unknown function (DUF543)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010209
Physical Location & Seq
Forward (+)
27156321 .. 27157809
1489 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G72170.1

Sequence Viewer

Length: 309 bp
ATGGCGGAGAAAAAGGATAGTGTGCCACCGGAATACGATGTGAATGCTAAGTGGGACGCATGTCTCGATCTCACTGTCCGCCGCTTCGTTTACTCTTCCCTCGGCGGCGCATTTGCCGGTCTTCTGTTCTTCAGGAGTCCTGTTACAAGATGGGCATCGATTGCTTTAGGTGCTGGAATTGGTATTGGCTCTGCATATTCAGATTGTTCTCGTGCTTTTGATTCACCTTCTTCATCTTCAGCTAATTTAGCAGCTCCCAAGAATAATATTACAGAGACTTCTCCTGTATCTCAGGCTGCAGACGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF543

Protein Analysis

102

Amino Acids

10.75

Weight (kDa)

4.98

Isoelectric Point (pI)

77.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF543 PF04418 3 - 74 1.7e-24 Domain of unknown function (DUF543)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 5, 79, 82, 105
AcuI CTGAAG 2 cut(s) 115, 222
AluBI AGCT 2 cut(s) 242, 254
AluI AGCT 2 cut(s) 242, 254
Alw26I GTCTC 2 cut(s) 68, 269
ApeKI GCWGC 2 cut(s) 251, 296
AspLEI GCGC 1 cut(s) 110
AsuHPI GGTGA 1 cut(s) 216
BauI CACGAG 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 113
BbvI GCAGC 2 cut(s) 263, 283
BccI CCATC 1 cut(s) 144
BcgI CGANNNNNNTGC 2 cut(s) 26, 60
BcoDI GTCTC 2 cut(s) 68, 269
BfmI CTRYAG 1 cut(s) 297
BisI GCNGC 4 cut(s) 82, 106, 252, 297
BlsI GCNGC 4 cut(s) 83, 107, 253, 298
BmsI GCATC 1 cut(s) 164
BoxI GACNNNNGTC 1 cut(s) 60
BpiI GAAGAC 1 cut(s) 113
Bsa29I ATCGAT 1 cut(s) 158
BsaBI GATNNNNATC 1 cut(s) 154
BsaJI CCNNGG 1 cut(s) 100
BsaWI WCCGGW 1 cut(s) 28
Bse118I RCCGGY 1 cut(s) 116
Bse8I GATNNNNATC 1 cut(s) 154
BseCI ATCGAT 1 cut(s) 158
BseDI CCNNGG 1 cut(s) 100
BseJI GATNNNNATC 1 cut(s) 154
BseMII CTCAG 1 cut(s) 305
BseXI GCAGC 2 cut(s) 263, 283
BshVI ATCGAT 1 cut(s) 158
BsiSI CCGG 2 cut(s) 29, 117
BslFI GGGAC 1 cut(s) 68
BsmAI GTCTC 2 cut(s) 68, 269
BsmFI GGGAC 1 cut(s) 68
BsmI GAATGC 1 cut(s) 49
Bsp143I GATC 1 cut(s) 67
BspACI CCGC 4 cut(s) 5, 79, 82, 105
BspCNI CTCAG 1 cut(s) 304
BspDI ATCGAT 1 cut(s) 158
BspMAI CTGCAG 1 cut(s) 301
BsrFI RCCGGY 1 cut(s) 116
BssAI RCCGGY 1 cut(s) 116
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 1 cut(s) 67
BssSI CACGAG 1 cut(s) 210
Bst2BI CACGAG 1 cut(s) 210
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 1 cut(s) 100
BstAPI GCANNNNNTGC 1 cut(s) 161
BstDEI CTNAG 2 cut(s) 48, 291
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 1 cut(s) 70
BstMAI GTCTC 2 cut(s) 68, 269
BstMBI GATC 1 cut(s) 67
BstMWI GCNNNNNNNGC 3 cut(s) 161, 170, 248
BstNSI RCATGY 1 cut(s) 63
BstPAI GACNNNNGTC 1 cut(s) 60
BstSFI CTRYAG 1 cut(s) 297
BstV1I GCAGC 2 cut(s) 263, 283
BstV2I GAAGAC 1 cut(s) 113
Bsu15I ATCGAT 1 cut(s) 158
BsuTUI ATCGAT 1 cut(s) 158
BtsIMutI CAGTG 1 cut(s) 72
CfoI GCGC 1 cut(s) 110
Cfr10I RCCGGY 1 cut(s) 116
ClaI ATCGAT 1 cut(s) 158
CseI GACGC 1 cut(s) 65
CviAII CATG 1 cut(s) 60
CviJI RGCY 4 cut(s) 189, 242, 254, 296
CviKI_1 RGCY 4 cut(s) 189, 242, 254, 296
DdeI CTNAG 2 cut(s) 48, 291
DpnI GATC 1 cut(s) 69
DpnII GATC 1 cut(s) 67
Eam1104I CTCTTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 100
EciI GGCGGA 2 cut(s) 20, 68
Eco57I CTGAAG 2 cut(s) 115, 222
FaeI CATG 1 cut(s) 63
FaiI YATR 2 cut(s) 61, 196
FaqI GGGAC 1 cut(s) 68
FatI CATG 1 cut(s) 59
Fnu4HI GCNGC 4 cut(s) 82, 106, 252, 297
Fsp4HI GCNGC 4 cut(s) 82, 106, 252, 297
GlaI GCGC 1 cut(s) 109
GluI GCNGC 4 cut(s) 82, 106, 252, 297
HapII CCGG 2 cut(s) 29, 117
HgaI GACGC 1 cut(s) 65
HhaI GCGC 1 cut(s) 110
Hin1II CATG 1 cut(s) 63
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HinfI GANTC 2 cut(s) 136, 221
HpaII CCGG 2 cut(s) 29, 117
HphI GGTGA 1 cut(s) 216
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 1 cut(s) 202
Hpy188III TCNNGA 2 cut(s) 65, 133
Hpy8I GTNNAC 1 cut(s) 91
HpyAV CCTTC 1 cut(s) 237
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4V TGCA 2 cut(s) 194, 299
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 170, 248
HpyF3I CTNAG 2 cut(s) 48, 291
Hsp92II CATG 1 cut(s) 63
HspAI GCGC 1 cut(s) 108
Kzo9I GATC 1 cut(s) 67
LmnI GCTCC 1 cut(s) 259
LpnPI CCDG 7 cut(s) 42, 118, 130, 153, 159, 278, 297
Lsp1109I GCAGC 2 cut(s) 263, 283
LweI GCATC 1 cut(s) 164
MaeIII GTNAC 1 cut(s) 142
MalI GATC 1 cut(s) 69
MboI GATC 1 cut(s) 67
MboII GAAGA 5 cut(s) 87, 113, 121, 222, 228
MluCI AATT 2 cut(s) 177, 244
MlyI GAGTC 1 cut(s) 145
MnlI CCTC 1 cut(s) 110
MspI CCGG 2 cut(s) 29, 117
Mva1269I GAATGC 1 cut(s) 49
MwoI GCNNNNNNNGC 3 cut(s) 161, 170, 248
NdeII GATC 1 cut(s) 67
NlaIII CATG 1 cut(s) 63
NmeAIII GCCGAG 1 cut(s) 81
NspI RCATGY 1 cut(s) 63
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 1 cut(s) 49
PfeI GAWTC 1 cut(s) 221
PkrI GCNGC 4 cut(s) 83, 107, 253, 298
PleI GAGTC 1 cut(s) 144
PpsI GAGTC 1 cut(s) 144
PshAI GACNNNNGTC 1 cut(s) 60
PstI CTGCAG 1 cut(s) 301
SatI GCNGC 4 cut(s) 82, 106, 252, 297
Sau3AI GATC 1 cut(s) 67
SchI GAGTC 1 cut(s) 145
SetI ASST 4 cut(s) 172, 229, 244, 256
SfaNI GCATC 1 cut(s) 164
SfcI CTRYAG 1 cut(s) 297
Sse9I AATT 2 cut(s) 177, 244
SsiI CCGC 4 cut(s) 5, 79, 82, 105
SspI AATATT 1 cut(s) 268
TaaI ACNGT 1 cut(s) 76
TaqI TCGA 2 cut(s) 66, 158
TasI AATT 2 cut(s) 177, 244
TauI GCSGC 2 cut(s) 84, 108
TfiI GAWTC 1 cut(s) 221
TscAI CASTG 1 cut(s) 79
TseI GCWGC 2 cut(s) 251, 296
TspDTI ATGAA 1 cut(s) 222
TspRI CASTG 1 cut(s) 79
XceI RCATGY 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.