FvH4_5g31700

Domain of unknown function (DUF543)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010209
Physical Location & Seq
Reverse (-)
22848947 .. 22851605
2659 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g31700.t2

Sequence Viewer

Length: 297 bp
ATGGTCGATAACAAAGAAATCCTGCCGCCGCAGTACGACGTCGACGCCAAAATGGACGCCTGTATCGATCTCACCGTCCGCCGCTTCGTCTACTCCACCCTCGGCGGTGCCATCGGCGGTCTTCTTTTGTTCAGGAGTCCTGTGACTCGCTGGGCTTCTGTAGCCTTTGGTGCTGGAATGGGCATTGGATCTGCATACACCGATTGTTCTCGTTTATTTGAGGGGTTGCCTTCAACTGCAAAGGTGGCATCACCTAAGATTGATGAGACTCCTGCTACTCAGGATGGACAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF543

Protein Analysis

99

Amino Acids

10.44

Weight (kDa)

4.73

Isoelectric Point (pI)

60.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF543 PF04418 7 - 73 3.4e-20 Domain of unknown function (DUF543)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 42
AccB1I GGYRCC 1 cut(s) 107
AccI GTMKAC 2 cut(s) 42, 90
AciI CCGC 6 cut(s) 26, 29, 79, 82, 105, 117
AclWI GGATC 1 cut(s) 196
AcyI GRCGYC 3 cut(s) 39, 45, 57
AfaI GTAC 1 cut(s) 35
AgsI TTSAA 1 cut(s) 234
Alw26I GTCTC 1 cut(s) 260
AlwI GGATC 1 cut(s) 196
AsuHPI GGTGA 2 cut(s) 64, 243
BaeI ACNNNNGTAYC 2 cut(s) 46, 79
BanI GGYRCC 1 cut(s) 107
BbsI GAAGAC 1 cut(s) 113
BccI CCATC 2 cut(s) 119, 278
BcoDI GTCTC 1 cut(s) 260
BfmI CTRYAG 1 cut(s) 159
BisI GCNGC 3 cut(s) 26, 29, 82
BlsI GCNGC 3 cut(s) 27, 30, 83
BmiI GGNNCC 1 cut(s) 109
BmsI GCATC 1 cut(s) 257
BpiI GAAGAC 1 cut(s) 113
Bsa29I ATCGAT 1 cut(s) 66
BsaHI GRCGYC 3 cut(s) 39, 45, 57
BsaJI CCNNGG 1 cut(s) 100
BseCI ATCGAT 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 1 cut(s) 289
BseMII CTCAG 1 cut(s) 293
BseYI CCCAGC 1 cut(s) 150
BshNI GGYRCC 1 cut(s) 107
BshVI ATCGAT 1 cut(s) 66
BsmAI GTCTC 1 cut(s) 260
Bsp143I GATC 2 cut(s) 67, 188
BspACI CCGC 6 cut(s) 26, 29, 79, 82, 105, 117
BspCNI CTCAG 1 cut(s) 292
BspDI ATCGAT 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 109
BspPI GGATC 1 cut(s) 196
BspT107I GGYRCC 1 cut(s) 107
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 2 cut(s) 67, 188
BssNI GRCGYC 3 cut(s) 39, 45, 57
Bst4CI ACNGT 1 cut(s) 76
BstACI GRCGYC 3 cut(s) 39, 45, 57
BstDEI CTNAG 2 cut(s) 255, 279
BstF5I GGATG 1 cut(s) 289
BstKTI GATC 2 cut(s) 70, 191
BstMAI GTCTC 1 cut(s) 260
BstMBI GATC 2 cut(s) 67, 188
BstMWI GCNNNNNNNGC 3 cut(s) 161, 170, 245
BstSFI CTRYAG 1 cut(s) 159
BstV2I GAAGAC 1 cut(s) 113
BstX2I RGATCY 1 cut(s) 188
BstYI RGATCY 1 cut(s) 188
Bsu15I ATCGAT 1 cut(s) 66
BsuTUI ATCGAT 1 cut(s) 66
BtsCI GGATG 1 cut(s) 289
ClaI ATCGAT 1 cut(s) 66
CseI GACGC 2 cut(s) 53, 65
Csp6I GTAC 1 cut(s) 34
CviJI RGCY 2 cut(s) 155, 164
CviKI_1 RGCY 2 cut(s) 155, 164
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 2 cut(s) 255, 279
DpnI GATC 2 cut(s) 69, 190
DpnII GATC 2 cut(s) 67, 188
EciI GGCGGA 1 cut(s) 68
FaiI YATR 1 cut(s) 196
FblI GTMKAC 2 cut(s) 42, 90
Fnu4HI GCNGC 3 cut(s) 26, 29, 82
Fsp4HI GCNGC 3 cut(s) 26, 29, 82
GluI GCNGC 3 cut(s) 26, 29, 82
GsaI CCCAGC 1 cut(s) 154
HgaI GACGC 2 cut(s) 53, 65
Hin1I GRCGYC 3 cut(s) 39, 45, 57
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 3 cut(s) 136, 145, 268
HphI GGTGA 2 cut(s) 64, 243
Hpy166II GTNNAC 2 cut(s) 43, 91
Hpy188III TCNNGA 2 cut(s) 133, 281
Hpy8I GTNNAC 2 cut(s) 43, 91
Hpy99I CGWCG 3 cut(s) 41, 44, 47
HpyAV CCTTC 1 cut(s) 240
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 2 cut(s) 194, 239
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 170, 245
HpyF3I CTNAG 2 cut(s) 255, 279
HpySE526I ACGT 1 cut(s) 39
Hsp92I GRCGYC 3 cut(s) 39, 45, 57
Kzo9I GATC 2 cut(s) 67, 188
LpnPI CCDG 9 cut(s) 35, 73, 118, 136, 153, 159, 266, 275, 285
LweI GCATC 1 cut(s) 257
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 142
MalI GATC 2 cut(s) 69, 190
MboI GATC 2 cut(s) 67, 188
MboII GAAGA 1 cut(s) 113
MflI RGATCY 1 cut(s) 188
MlyI GAGTC 3 cut(s) 139, 145, 262
MnlI CCTC 2 cut(s) 110, 214
MseI TTAA 1 cut(s) 295
MwoI GCNNNNNNNGC 3 cut(s) 161, 170, 245
NdeII GATC 2 cut(s) 67, 188
NlaIV GGNNCC 1 cut(s) 109
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 1 cut(s) 142
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 72
PkrI GCNGC 3 cut(s) 27, 30, 83
PleI GAGTC 3 cut(s) 139, 144, 262
PpsI GAGTC 3 cut(s) 139, 144, 262
PspFI CCCAGC 1 cut(s) 150
PspN4I GGNNCC 1 cut(s) 109
PsuI RGATCY 1 cut(s) 188
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 295
SatI GCNGC 3 cut(s) 26, 29, 82
Sau3AI GATC 2 cut(s) 67, 188
SchI GAGTC 3 cut(s) 139, 145, 262
SetI ASST 3 cut(s) 42, 246, 256
SfaNI GCATC 1 cut(s) 257
SfcI CTRYAG 1 cut(s) 159
SgrDI CGTCGACG 1 cut(s) 41
SsiI CCGC 6 cut(s) 26, 29, 79, 82, 105, 117
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 1 cut(s) 42
TaqI TCGA 3 cut(s) 6, 42, 66
TauI GCSGC 3 cut(s) 28, 31, 84
Tru1I TTAA 1 cut(s) 295
Tru9I TTAA 1 cut(s) 295
TseFI GTSAC 1 cut(s) 142
Tsp45I GTSAC 1 cut(s) 142
XmiI GTMKAC 2 cut(s) 42, 90
ZraI GACGTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.