MD08G1217900.v1.1

Domain of unknown function (DUF543)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010209
Physical Location & Seq
Reverse (-)
28051344 .. 28054775
3432 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1217900.v1.1.491

Sequence Viewer

Length: 315 bp
ATGGCGGACAACAAAGAAATCATCCCACCGCCGTACGACGTGAACGCCAAATGGGACGCCTGTCTCGATCTGACCGTCCGCCGCTTCGTCTACTCCTCCTTGGGTGGTGCCTTTGGCGGTCTCCTTTTGTTCAGAAGTCCTGTGTCTCGTTGGGCTTCTGTAGCTTTTGGTGCTGGATTGGGCATTGGATCTGCATACACAGAGTGCTCTCGTTTGTTTGAGGGATCTCCAGCAAAGTCGGCTGCTCCTAAGATGGTTGAGAATCCTGCTCCTAAGATTGTTGAGAATCCTGCTCCTCAGGACGGACAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF543

Protein Analysis

105

Amino Acids

11.06

Weight (kDa)

5.33

Isoelectric Point (pI)

77.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF543 PF04418 5 - 73 5.8e-22 Domain of unknown function (DUF543)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 107
AccI GTMKAC 1 cut(s) 90
AciI CCGC 5 cut(s) 5, 29, 79, 82, 117
AclWI GGATC 2 cut(s) 196, 232
AcyI GRCGYC 1 cut(s) 57
AdeI CACNNNGTG 1 cut(s) 204
AfaI GTAC 1 cut(s) 35
AfiI CCNNNNNNNGG 1 cut(s) 302
AjiI CACGTC 1 cut(s) 40
AluBI AGCT 1 cut(s) 164
AluI AGCT 1 cut(s) 164
Alw21I GWGCWC 1 cut(s) 209
Alw26I GTCTC 3 cut(s) 68, 125, 150
AlwI GGATC 2 cut(s) 196, 232
ApeKI GCWGC 1 cut(s) 242
AxyI CCTNAGG 1 cut(s) 297
BanI GGYRCC 1 cut(s) 107
Bbv12I GWGCWC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 229
BccI CCATC 1 cut(s) 247
BceAI ACGGC 1 cut(s) 16
BcoDI GTCTC 3 cut(s) 68, 125, 150
BfmI CTRYAG 1 cut(s) 159
BisI GCNGC 2 cut(s) 82, 243
BlsI GCNGC 2 cut(s) 83, 244
BmgBI CACGTC 1 cut(s) 40
BmiI GGNNCC 1 cut(s) 109
BoxI GACNNNNGTC 1 cut(s) 60
BpmI CTGGAG 1 cut(s) 213
BsaHI GRCGYC 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 1 cut(s) 99
Bsc4I CCNNNNNNNGG 1 cut(s) 302
Bse21I CCTNAGG 1 cut(s) 297
BseDI CCNNGG 1 cut(s) 99
BseGI GGATG 1 cut(s) 21
BseLI CCNNNNNNNGG 1 cut(s) 302
BseMII CTCAG 1 cut(s) 311
BseRI GAGGAG 2 cut(s) 85, 285
BseXI GCAGC 1 cut(s) 229
BshNI GGYRCC 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 209
BsiWI CGTACG 1 cut(s) 33
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 1 cut(s) 302
BsmAI GTCTC 3 cut(s) 68, 125, 150
BsmFI GGGAC 1 cut(s) 68
Bso31I GGTCTC 1 cut(s) 125
Bsp1286I GDGCHC 1 cut(s) 209
Bsp143I GATC 3 cut(s) 67, 188, 224
BspACI CCGC 5 cut(s) 5, 29, 79, 82, 117
BspCNI CTCAG 1 cut(s) 310
BspLI GGNNCC 1 cut(s) 109
BspPI GGATC 2 cut(s) 196, 232
BspT107I GGYRCC 1 cut(s) 107
BspTNI GGTCTC 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 3 cut(s) 67, 188, 224
BssNI GRCGYC 1 cut(s) 57
BssT1I CCWWGG 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 76
BstACI GRCGYC 1 cut(s) 57
BstDEI CTNAG 3 cut(s) 249, 273, 297
BstF5I GGATG 1 cut(s) 21
BstKTI GATC 3 cut(s) 70, 191, 227
BstMAI GTCTC 3 cut(s) 68, 125, 150
BstMBI GATC 3 cut(s) 67, 188, 224
BstMWI GCNNNNNNNGC 3 cut(s) 161, 170, 239
BstPAI GACNNNNGTC 1 cut(s) 60
BstSFI CTRYAG 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 229
BstX2I RGATCY 2 cut(s) 188, 224
BstYI RGATCY 2 cut(s) 188, 224
Bsu36I CCTNAGG 1 cut(s) 297
BtrI CACGTC 1 cut(s) 40
BtsCI GGATG 1 cut(s) 21
CseI GACGC 1 cut(s) 65
Csp6I GTAC 1 cut(s) 34
CviJI RGCY 3 cut(s) 155, 164, 242
CviKI_1 RGCY 3 cut(s) 155, 164, 242
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 3 cut(s) 249, 273, 297
DpnI GATC 3 cut(s) 69, 190, 226
DpnII GATC 3 cut(s) 67, 188, 224
DraIII CACNNNGTG 1 cut(s) 204
EciI GGCGGA 2 cut(s) 20, 68
Eco130I CCWWGG 1 cut(s) 99
Eco31I GGTCTC 1 cut(s) 125
Eco81I CCTNAGG 1 cut(s) 297
EcoT14I CCWWGG 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 99
FaiI YATR 1 cut(s) 196
FaqI GGGAC 1 cut(s) 68
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 2 cut(s) 82, 243
FokI GGATG 1 cut(s) 8
Fsp4HI GCNGC 2 cut(s) 82, 243
GluI GCNGC 2 cut(s) 82, 243
GsuI CTGGAG 1 cut(s) 213
HgaI GACGC 1 cut(s) 65
Hin1I GRCGYC 1 cut(s) 57
HinfI GANTC 2 cut(s) 262, 286
Hpy166II GTNNAC 2 cut(s) 43, 91
Hpy188I TCNGA 2 cut(s) 72, 134
Hpy188III TCNNGA 2 cut(s) 65, 299
Hpy8I GTNNAC 2 cut(s) 43, 91
Hpy99I CGWCG 1 cut(s) 41
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 1 cut(s) 194
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 170, 239
HpyF3I CTNAG 3 cut(s) 249, 273, 297
HpySE526I ACGT 1 cut(s) 39
Hsp92I GRCGYC 1 cut(s) 57
Kzo9I GATC 3 cut(s) 67, 188, 224
LmnI GCTCC 3 cut(s) 250, 274, 298
LpnPI CCDG 7 cut(s) 73, 153, 159, 243, 279, 284, 303
Lsp1109I GCAGC 1 cut(s) 229
MaeII ACGT 1 cut(s) 39
MalI GATC 3 cut(s) 69, 190, 226
MboI GATC 3 cut(s) 67, 188, 224
MflI RGATCY 2 cut(s) 188, 224
MhlI GDGCHC 1 cut(s) 209
MnlI CCTC 3 cut(s) 106, 214, 306
MwoI GCNNNNNNNGC 3 cut(s) 161, 170, 239
NdeII GATC 3 cut(s) 67, 188, 224
NlaIV GGNNCC 1 cut(s) 109
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 72
PfeI GAWTC 2 cut(s) 262, 286
Pfl23II CGTACG 1 cut(s) 33
PkrI GCNGC 2 cut(s) 83, 244
PshAI GACNNNNGTC 1 cut(s) 60
PspLI CGTACG 1 cut(s) 33
PspN4I GGNNCC 1 cut(s) 109
PsuI RGATCY 2 cut(s) 188, 224
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
SatI GCNGC 2 cut(s) 82, 243
Sau3AI GATC 3 cut(s) 67, 188, 224
SduI GDGCHC 1 cut(s) 209
SetI ASST 2 cut(s) 42, 166
SfcI CTRYAG 1 cut(s) 159
SsiI CCGC 5 cut(s) 5, 29, 79, 82, 117
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 1 cut(s) 42
TaqI TCGA 1 cut(s) 66
TauI GCSGC 1 cut(s) 84
TfiI GAWTC 2 cut(s) 262, 286
TseI GCWGC 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.