Rh7BG377000

Domain of unknown function (DUF543)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0010209
Physical Location & Seq
Reverse (-)
40547048 .. 40552070
5023 bp
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UTR
Exon/CDS
Intron
Rh7BG377000.1

Sequence Viewer

Length: 297 bp
ATGGCCGATAACCGAGAAATCCTGCCGCCGCAGTACGACGTCAACGCCAAATGGGACGCCTGTATCGATCTCACCGTCCGCCGCTTCGTCTACTCTACCCTCGGCGGAGCCGTCGGCGGTCTTCTTCTCTTCAGGAGTCCTGTGACTCGCTGGGCTGCTGTAGCCTTTGGTGCTGGATTGGGCATTGGATCTGCATACACAGATTGTTCTCGTTTATTTGAGGGATCTCCTGCAACTGCAAAGTTGGCATCTCATAAGATTGTTGAGACTCCTGCTCCTCAGGATGGACAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF543

Protein Analysis

98

Amino Acids

10.44

Weight (kDa)

5.69

Isoelectric Point (pI)

61.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF543 PF04418 8 - 73 7.9e-22 Domain of unknown function (DUF543)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 42
AccI GTMKAC 1 cut(s) 90
AciI CCGC 6 cut(s) 26, 29, 79, 82, 105, 117
AclWI GGATC 2 cut(s) 196, 232
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 115
AcyI GRCGYC 2 cut(s) 39, 57
AfaI GTAC 1 cut(s) 35
AfiI CCNNNNNNNGG 1 cut(s) 284
Alw26I GTCTC 1 cut(s) 260
AlwI GGATC 2 cut(s) 196, 232
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 155
AsuHPI GGTGA 1 cut(s) 64
AxyI CCTNAGG 1 cut(s) 279
BaeI ACNNNNGTAYC 2 cut(s) 46, 79
BbsI GAAGAC 1 cut(s) 113
BbvI GCAGC 1 cut(s) 142
BccI CCATC 1 cut(s) 278
BceAI ACGGC 1 cut(s) 95
BcoDI GTCTC 1 cut(s) 260
BfmI CTRYAG 1 cut(s) 159
BisI GCNGC 4 cut(s) 26, 29, 82, 156
BlsI GCNGC 4 cut(s) 27, 30, 83, 157
BmiI GGNNCC 1 cut(s) 109
BmsI GCATC 1 cut(s) 257
BpiI GAAGAC 1 cut(s) 113
Bsa29I ATCGAT 1 cut(s) 66
BsaHI GRCGYC 2 cut(s) 39, 57
BsaJI CCNNGG 1 cut(s) 100
BsaXI ACNNNNNCTCC 2 cut(s) 259, 289
Bsc4I CCNNNNNNNGG 1 cut(s) 284
Bse21I CCTNAGG 1 cut(s) 279
BseCI ATCGAT 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 1 cut(s) 284
BseMII CTCAG 1 cut(s) 293
BseRI GAGGAG 1 cut(s) 267
BseXI GCAGC 1 cut(s) 142
BseYI CCCAGC 1 cut(s) 150
BshFI GGCC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 66
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 1 cut(s) 284
BsmAI GTCTC 1 cut(s) 260
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 3 cut(s) 67, 188, 224
BspACI CCGC 6 cut(s) 26, 29, 79, 82, 105, 117
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 292
BspDI ATCGAT 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 109
BspPI GGATC 2 cut(s) 196, 232
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 3 cut(s) 67, 188, 224
BssNI GRCGYC 2 cut(s) 39, 57
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 1 cut(s) 134
BstACI GRCGYC 2 cut(s) 39, 57
BstDEI CTNAG 1 cut(s) 279
BstF5I GGATG 1 cut(s) 289
BstKTI GATC 3 cut(s) 70, 191, 227
BstMAI GTCTC 1 cut(s) 260
BstMBI GATC 3 cut(s) 67, 188, 224
BstMWI GCNNNNNNNGC 3 cut(s) 161, 170, 245
BstSFI CTRYAG 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 142
BstV2I GAAGAC 1 cut(s) 113
BstX2I RGATCY 2 cut(s) 188, 224
BstYI RGATCY 2 cut(s) 188, 224
Bsu15I ATCGAT 1 cut(s) 66
Bsu36I CCTNAGG 1 cut(s) 279
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 1 cut(s) 66
BtsCI GGATG 1 cut(s) 289
ClaI ATCGAT 1 cut(s) 66
CseI GACGC 1 cut(s) 65
Csp6I GTAC 1 cut(s) 34
CviJI RGCY 4 cut(s) 5, 110, 155, 164
CviKI_1 RGCY 4 cut(s) 5, 110, 155, 164
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 1 cut(s) 279
DpnI GATC 3 cut(s) 69, 190, 226
DpnII GATC 3 cut(s) 67, 188, 224
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
EciI GGCGGA 2 cut(s) 68, 120
Eco57I CTGAAG 1 cut(s) 115
Eco81I CCTNAGG 1 cut(s) 279
FaiI YATR 2 cut(s) 196, 255
FaqI GGGAC 1 cut(s) 68
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 4 cut(s) 26, 29, 82, 156
Fsp4HI GCNGC 4 cut(s) 26, 29, 82, 156
GluI GCNGC 4 cut(s) 26, 29, 82, 156
GsaI CCCAGC 1 cut(s) 154
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 1 cut(s) 65
Hin1I GRCGYC 2 cut(s) 39, 57
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 3 cut(s) 136, 145, 268
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 2 cut(s) 43, 91
Hpy188III TCNNGA 2 cut(s) 133, 281
Hpy8I GTNNAC 2 cut(s) 43, 91
Hpy99I CGWCG 2 cut(s) 41, 116
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 3 cut(s) 194, 233, 239
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 170, 245
HpyF3I CTNAG 1 cut(s) 279
HpySE526I ACGT 1 cut(s) 39
Hsp92I GRCGYC 2 cut(s) 39, 57
Kzo9I GATC 3 cut(s) 67, 188, 224
LmnI GCTCC 2 cut(s) 107, 280
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 1 cut(s) 257
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 142
MalI GATC 3 cut(s) 69, 190, 226
MboI GATC 3 cut(s) 67, 188, 224
MboII GAAGA 3 cut(s) 113, 116, 121
MflI RGATCY 2 cut(s) 188, 224
MlyI GAGTC 3 cut(s) 139, 145, 262
MnlI CCTC 3 cut(s) 110, 214, 288
MwoI GCNNNNNNNGC 3 cut(s) 161, 170, 245
NdeII GATC 3 cut(s) 67, 188, 224
NlaIV GGNNCC 1 cut(s) 109
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 1 cut(s) 142
PcsI WCGNNNNNNNCGW 3 cut(s) 63, 72, 108
PkrI GCNGC 4 cut(s) 27, 30, 83, 157
PleI GAGTC 3 cut(s) 139, 144, 262
PpsI GAGTC 3 cut(s) 139, 144, 262
PspFI CCCAGC 1 cut(s) 150
PspN4I GGNNCC 1 cut(s) 109
PsuI RGATCY 2 cut(s) 188, 224
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
SatI GCNGC 4 cut(s) 26, 29, 82, 156
Sau3AI GATC 3 cut(s) 67, 188, 224
SchI GAGTC 3 cut(s) 139, 145, 262
SetI ASST 1 cut(s) 42
SfaNI GCATC 1 cut(s) 257
SfcI CTRYAG 1 cut(s) 159
SsiI CCGC 6 cut(s) 26, 29, 79, 82, 105, 117
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 1 cut(s) 42
TaqI TCGA 1 cut(s) 66
TauI GCSGC 3 cut(s) 28, 31, 84
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 1 cut(s) 155
Tsp45I GTSAC 1 cut(s) 142
XmiI GTMKAC 1 cut(s) 90
ZraI GACGTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.