AT1G22600

Late embryogenesis abundant domain-containing protein LEA domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010354
Physical Location & Seq
Reverse (-)
7987141 .. 7989217
2077 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G22600.1

Sequence Viewer

Length: 1158 bp
ATGACAAAACTATTGGCCTTGTCTCTTGTGTTGAGTGCGTTATTTACAGTGGGCCACTGCACGGGTTCTGAGGATGACATCATCGTGAGAGACGGCCACCGTGTTGTTGTGGTGGAGTACGACAGAGACGGCGAAACAAACACTAGAGTCTTGATTTCACCTCCGGGGAAAGAAGTAGATCAGAAGAGCAGAGACGAAGGAGAAGTGTTTGGAAATGAGAAGAGAGAAACGGCGTCGTCTCTTCCCGAAGAAGAGGAGCGTGAACACCACGCGACGCCAGGGGAACTTATATGCGATGCAATTGGTAAGTGTAAGCACAAGCTGGGGACTGTGTTAGGGAGAGTCAAAGATCGAACGGCTAGTGATCTTTCCGACGAAACGCCTGAAATGACGGTCGCACGTGAGGCACTTGAAGTGGAAGAGAAGGTCTCCTGGAAAGCCCGTGAAGCTAGGGGAAAGGTAAACGAGAGGGCCACAAAGAAGGCTCATAGGGTCCAAAAAGTTTTGGAGAAGGTACAGATCGCGGTGCGGGGGATAGGAACGGTGGTTGCGACGGCGCTGGGGCTGACGAAGATCGGGAGTGTGGTGGGAATTGTCGGGATCGCGGCGGCGTATGGGATGTGCGTGTGGGTGACGTTTGTGTCGGGCTACGTGTTGGCTTCGGTTCTTGGGGAGCAGCAATTCGGTGTGGTGCAGAGCAAGATGTATCCGGTGTACTTCAAGGCAGTCTCTGTAGGGATTTTGGTTGGGTTGTTAGGACACGTCATAGGCCGACGCCGGAAGGTTTTTACCGATGCGGTGGATATGTGGCAATCCGTTAACCTCTTGTCCTCTATCTTGATGGTCGAAGCTAATGCGTCATTTGTTTACACACGAGCCACCAAGGCCATGTTTGAGCTGATAAAAGCTGAAAAGGAAGATGGAAGAGGATTTGACACGTCAGACCAGTCACAGTCTAGTGAATCTGCCGGTCGAACCAGAGGAAAGAAGAAGGTTACAGAGAAGACGGATGAGGATGTGGTTAAGCAGAGACTGACAAAGCTGAGCGAGAGGATGCGTAAGTTAAATGCGTACTCTTCGCGGCTAAATTTACTTACTCTCATGTCTCTCACTTGGCACTTTGTGTACCTTGGCTACCGACTTAGCCTTACTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4149

Protein Analysis

385

Amino Acids

42.53

Weight (kDa)

8.9

Isoelectric Point (pI)

26.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4149 PF13664 201 - 299 7e-21 Domain of unknown function (DUF4149)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22600 AT1G72100
fragaria_vesca FvH4_5g31970
malus_domestica MD15G1396400.v1.1 MD15G1407700.v1.1
prunus_persica Prupe.1G554000_v2.0.a1
pyrus_communis pycom15g36370
rosa_chinensis RchiOBHm_Chr7g0227991
rosa_laevigata RLG00000001601
rosa_multiflora Rmu_co8508245.1_g000001 Rmu_sc0006493.1_g000005
rosa_roxburghii Rroxscaffold_3G00231680
rosa_rugosa Rorug07G0244900
rosa_samantha Rh7AG398000 Rh7AG398300 Rh7AG398800 Rh7BG380100 Rh7CG416400 Rh7CG416900 Rh7DG394400
rosa_wichuraiana Rw7G033150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 640
AccII CGCG 4 cut(s) 272, 524, 605, 1081
AciI CCGC 6 cut(s) 524, 529, 605, 608, 797, 1081
AclWI GGATC 1 cut(s) 608
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 1 cut(s) 1087
AcvI CACGTG 1 cut(s) 401
AcyI GRCGYC 3 cut(s) 233, 275, 775
AdeI CACNNNGTG 1 cut(s) 1123
AfaI GTAC 5 cut(s) 119, 516, 716, 1073, 1127
AfiI CCNNNNNNNGG 1 cut(s) 61
AflIII ACRYGT 3 cut(s) 651, 760, 936
AgsI TTSAA 2 cut(s) 413, 721
AjiI CACGTC 2 cut(s) 763, 939
AjnI CCWGG 2 cut(s) 277, 431
AluBI AGCT 6 cut(s) 322, 449, 851, 898, 908, 1042
AluI AGCT 6 cut(s) 322, 449, 851, 898, 908, 1042
Alw26I GTCTC 9 cut(s) 27, 84, 120, 186, 243, 433, 733, 1024, 1110
AlwI GGATC 1 cut(s) 608
AlwNI CAGNNNCTG 2 cut(s) 731, 1033
AoxI GGCC 6 cut(s) 15, 52, 94, 471, 769, 885
ApeKI GCWGC 1 cut(s) 676
ApoI RAATTY 1 cut(s) 1087
AspLEI GCGC 1 cut(s) 559
AspS9I GGNCC 3 cut(s) 52, 471, 493
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 2 cut(s) 150, 643
AvaII GGWCC 1 cut(s) 493
BauI CACGAG 1 cut(s) 873
BbrPI CACGTG 1 cut(s) 401
BbsI GAAGAC 1 cut(s) 1010
BbvI GCAGC 1 cut(s) 688
BccI CCATC 2 cut(s) 835, 914
BceAI ACGGC 5 cut(s) 109, 145, 246, 372, 570
BcgI CGANNNNNNTGC 2 cut(s) 836, 870
BciT130I CCWGG 2 cut(s) 279, 433
BciVI GTATCC 1 cut(s) 717
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 9 cut(s) 27, 84, 120, 186, 243, 433, 733, 1024, 1110
BfaI CTAG 4 cut(s) 144, 360, 450, 957
BfmI CTRYAG 1 cut(s) 732
BfoI RGCGCY 1 cut(s) 560
BfuI GTATCC 1 cut(s) 717
BglI GCCNNNNNGGC 1 cut(s) 884
BisI GCNGC 4 cut(s) 606, 609, 677, 1082
BlpI GCTNAGC 1 cut(s) 1043
BlsI GCNGC 4 cut(s) 607, 610, 678, 1083
Bme1390I CCNGG 3 cut(s) 165, 279, 433
Bme18I GGWCC 1 cut(s) 493
BmgBI CACGTC 2 cut(s) 763, 939
BmgT120I GGNCC 3 cut(s) 52, 471, 493
BmiI GGNNCC 1 cut(s) 494
BmrFI CCNGG 3 cut(s) 165, 279, 433
BmsI GCATC 3 cut(s) 286, 784, 1044
BpiI GAAGAC 1 cut(s) 1010
BplI GAGNNNNNCTC 2 cut(s) 413, 445
Bpu1102I GCTNAGC 1 cut(s) 1043
BpuMI CCSGG 1 cut(s) 165
BsaAI YACGTR 2 cut(s) 401, 652
BsaHI GRCGYC 3 cut(s) 233, 275, 775
BsaI GGTCTC 1 cut(s) 433
BsaJI CCNNGG 4 cut(s) 164, 278, 882, 1129
BsaWI WCCGGW 1 cut(s) 709
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse118I RCCGGY 1 cut(s) 968
Bse1I ACTGG 1 cut(s) 946
BseBI CCWGG 2 cut(s) 279, 433
BseDI CCNNGG 4 cut(s) 164, 278, 882, 1129
BseGI GGATG 5 cut(s) 79, 624, 1015, 1021, 1059
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMII CTCAG 2 cut(s) 60, 1034
BseNI ACTGG 1 cut(s) 946
BseRI GAGGAG 1 cut(s) 269
BseXI GCAGC 1 cut(s) 688
BseYI CCCAGC 2 cut(s) 322, 559
BsgI GTGCAG 2 cut(s) 43, 713
Bsh1236I CGCG 4 cut(s) 272, 524, 605, 1081
Bsh1285I CGRYCG 2 cut(s) 396, 973
BshFI GGCC 6 cut(s) 17, 54, 96, 473, 771, 887
BsiEI CGRYCG 2 cut(s) 396, 973
BsiSI CCGG 4 cut(s) 164, 710, 778, 969
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 9 cut(s) 27, 84, 120, 186, 243, 433, 733, 1024, 1110
BsmBI CGTCTC 4 cut(s) 84, 120, 186, 243
BsmFI GGGAC 1 cut(s) 340
BsnI GGCC 6 cut(s) 17, 54, 96, 473, 771, 887
Bso31I GGTCTC 1 cut(s) 433
Bsp143I GATC 6 cut(s) 178, 349, 364, 519, 573, 600
Bsp1720I GCTNAGC 1 cut(s) 1043
BspACI CCGC 6 cut(s) 524, 529, 605, 608, 797, 1081
BspANI GGCC 6 cut(s) 17, 54, 96, 473, 771, 887
BspCNI CTCAG 2 cut(s) 61, 1035
BspFNI CGCG 4 cut(s) 272, 524, 605, 1081
BspLI GGNNCC 1 cut(s) 494
BspPI GGATC 1 cut(s) 608
BspQI GCTCTTC 1 cut(s) 179
BspTNI GGTCTC 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 968
BsrI ACTGG 1 cut(s) 946
BssAI RCCGGY 1 cut(s) 968
BssECI CCNNGG 4 cut(s) 164, 278, 882, 1129
BssMI GATC 6 cut(s) 178, 349, 364, 519, 573, 600
BssNI GRCGYC 3 cut(s) 233, 275, 775
BssSI CACGAG 1 cut(s) 873
BssT1I CCWWGG 2 cut(s) 882, 1129
Bst2BI CACGAG 1 cut(s) 873
Bst2UI CCWGG 2 cut(s) 279, 433
Bst4CI ACNGT 6 cut(s) 49, 101, 331, 394, 544, 954
Bst6I CTCTTC 7 cut(s) 179, 215, 246, 246, 414, 919, 1081
BstACI GRCGYC 3 cut(s) 233, 275, 775
BstBAI YACGTR 2 cut(s) 401, 652
BstDEI CTNAG 3 cut(s) 69, 1043, 1142
BstF5I GGATG 5 cut(s) 79, 624, 1015, 1021, 1059
BstFNI CGCG 4 cut(s) 272, 524, 605, 1081
BstH2I RGCGCY 1 cut(s) 560
BstHHI GCGC 1 cut(s) 559
BstKTI GATC 6 cut(s) 181, 352, 367, 522, 576, 603
BstMAI GTCTC 9 cut(s) 27, 84, 120, 186, 243, 433, 733, 1024, 1110
BstMBI GATC 6 cut(s) 178, 349, 364, 519, 573, 600
BstMCI CGRYCG 2 cut(s) 396, 973
BstMWI GCNNNNNNNGC 3 cut(s) 404, 446, 884
BstNI CCWGG 2 cut(s) 279, 433
BstSCI CCNGG 3 cut(s) 163, 277, 431
BstSFI CTRYAG 1 cut(s) 732
BstUI CGCG 4 cut(s) 272, 524, 605, 1081
BstV1I GCAGC 1 cut(s) 688
BstV2I GAAGAC 1 cut(s) 1010
BsuI GTATCC 1 cut(s) 717
BsuRI GGCC 6 cut(s) 17, 54, 96, 473, 771, 887
BtgZI GCGATG 1 cut(s) 309
BtrI CACGTC 2 cut(s) 763, 939
BtsCI GGATG 5 cut(s) 79, 624, 1015, 1021, 1059
BtsI GCAGTG 1 cut(s) 55
BtsIMutI CAGTG 2 cut(s) 54, 55
CaiI CAGNNNCTG 2 cut(s) 731, 1033
CfoI GCGC 1 cut(s) 559
Cfr10I RCCGGY 1 cut(s) 968
Cfr13I GGNCC 3 cut(s) 52, 471, 493
CseI GACGC 4 cut(s) 222, 283, 783, 846
Csp6I GTAC 5 cut(s) 118, 515, 715, 1072, 1126
CviAII CATG 2 cut(s) 889, 1102
CviQI GTAC 5 cut(s) 118, 515, 715, 1072, 1126
DdeI CTNAG 3 cut(s) 69, 1043, 1142
DpnI GATC 6 cut(s) 180, 351, 366, 521, 575, 602
DpnII GATC 6 cut(s) 178, 349, 364, 519, 573, 600
DraIII CACNNNGTG 1 cut(s) 1123
DrdI GACNNNNNNGTC 1 cut(s) 640
DseDI GACNNNNNNGTC 1 cut(s) 640
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 7 cut(s) 179, 215, 246, 246, 414, 919, 1081
EarI CTCTTC 7 cut(s) 179, 215, 246, 246, 414, 919, 1081
Eco130I CCWWGG 2 cut(s) 882, 1129
Eco31I GGTCTC 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 493
Eco72I CACGTG 1 cut(s) 401
EcoRII CCWGG 2 cut(s) 277, 431
EcoT14I CCWWGG 2 cut(s) 882, 1129
ErhI CCWWGG 2 cut(s) 882, 1129
Esp3I CGTCTC 4 cut(s) 84, 120, 186, 243
FaeI CATG 2 cut(s) 892, 1105
FaiI YATR 8 cut(s) 290, 292, 489, 615, 767, 806, 890, 1103
FaqI GGGAC 1 cut(s) 340
FatI CATG 2 cut(s) 888, 1101
FauI CCCGC 1 cut(s) 522
Fnu4HI GCNGC 4 cut(s) 606, 609, 677, 1082
FokI GGATG 5 cut(s) 86, 631, 1022, 1028, 1066
Fsp4HI GCNGC 4 cut(s) 606, 609, 677, 1082
FspBI CTAG 4 cut(s) 144, 360, 450, 957
GlaI GCGC 1 cut(s) 558
GluI GCNGC 4 cut(s) 606, 609, 677, 1082
GsaI CCCAGC 2 cut(s) 326, 563
HaeII RGCGCY 1 cut(s) 560
HaeIII GGCC 6 cut(s) 17, 54, 96, 473, 771, 887
HapII CCGG 4 cut(s) 164, 710, 778, 969
HgaI GACGC 4 cut(s) 222, 283, 783, 846
HhaI GCGC 1 cut(s) 559
Hin1I GRCGYC 3 cut(s) 233, 275, 775
Hin1II CATG 2 cut(s) 892, 1105
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HinfI GANTC 3 cut(s) 147, 342, 962
HpaI GTTAAC 1 cut(s) 820
HpaII CCGG 4 cut(s) 164, 710, 778, 969
HphI GGTGA 2 cut(s) 150, 643
Hpy166II GTNNAC 6 cut(s) 263, 463, 715, 820, 868, 1126
Hpy188I TCNGA 4 cut(s) 70, 183, 373, 943
Hpy188III TCNNGA 6 cut(s) 85, 151, 245, 577, 598, 838
Hpy8I GTNNAC 6 cut(s) 263, 463, 715, 820, 868, 1126
Hpy99I CGWCG 5 cut(s) 238, 277, 377, 556, 777
HpyAV CCTTC 6 cut(s) 191, 418, 475, 505, 775, 985
HpyCH4III ACNGT 6 cut(s) 49, 101, 331, 394, 544, 954
HpyCH4IV ACGT 5 cut(s) 400, 635, 651, 762, 938
HpyCH4V TGCA 3 cut(s) 60, 299, 694
HpyF10VI GCNNNNNNNGC 3 cut(s) 404, 446, 884
HpyF3I CTNAG 3 cut(s) 69, 1043, 1142
HpySE526I ACGT 5 cut(s) 400, 635, 651, 762, 938
Hsp92I GRCGYC 3 cut(s) 233, 275, 775
Hsp92II CATG 2 cut(s) 892, 1105
HspAI GCGC 1 cut(s) 557
KspAI GTTAAC 1 cut(s) 820
Kzo9I GATC 6 cut(s) 178, 349, 364, 519, 573, 600
LguI GCTCTTC 1 cut(s) 179
LmnI GCTCC 2 cut(s) 256, 673
Lsp1109I GCAGC 1 cut(s) 688
LweI GCATC 3 cut(s) 286, 784, 1044
MaeI CTAG 4 cut(s) 144, 360, 450, 957
MaeII ACGT 5 cut(s) 400, 635, 651, 762, 938
MaeIII GTNAC 3 cut(s) 631, 948, 994
MalI GATC 6 cut(s) 180, 351, 366, 521, 575, 602
MboI GATC 6 cut(s) 178, 349, 364, 519, 573, 600
MfeI CAATTG 1 cut(s) 300
MluCI AATT 4 cut(s) 300, 591, 680, 1087
MlyI GAGTC 2 cut(s) 156, 351
MmeI TCCRAC 1 cut(s) 396
MseI TTAA 3 cut(s) 819, 1023, 1064
MslI CAYNNNNRTG 1 cut(s) 83
MspI CCGG 4 cut(s) 164, 710, 778, 969
MspR9I CCNGG 3 cut(s) 165, 279, 433
MunI CAATTG 1 cut(s) 300
MvaI CCWGG 2 cut(s) 279, 433
MvnI CGCG 4 cut(s) 272, 524, 605, 1081
MwoI GCNNNNNNNGC 3 cut(s) 404, 446, 884
NciI CCSGG 1 cut(s) 165
NdeII GATC 6 cut(s) 178, 349, 364, 519, 573, 600
NlaIII CATG 2 cut(s) 892, 1105
NlaIV GGNNCC 1 cut(s) 494
NmuCI GTSAC 2 cut(s) 631, 948
PciSI GCTCTTC 1 cut(s) 179
PcsI WCGNNNNNNNCGW 1 cut(s) 548
PfeI GAWTC 1 cut(s) 962
PfoI TCCNGGA 1 cut(s) 431
PkrI GCNGC 4 cut(s) 607, 610, 678, 1083
PleI GAGTC 2 cut(s) 155, 350
PmaCI CACGTG 1 cut(s) 401
PmlI CACGTG 1 cut(s) 401
PpsI GAGTC 2 cut(s) 155, 350
Ppu21I YACGTR 2 cut(s) 401, 652
Psp6I CCWGG 2 cut(s) 277, 431
PspCI CACGTG 1 cut(s) 401
PspFI CCCAGC 2 cut(s) 322, 559
PspGI CCWGG 2 cut(s) 277, 431
PspN4I GGNNCC 1 cut(s) 494
PspPI GGNCC 3 cut(s) 52, 471, 493
PstNI CAGNNNCTG 2 cut(s) 731, 1033
RsaI GTAC 5 cut(s) 119, 516, 716, 1073, 1127
RsaNI GTAC 5 cut(s) 118, 515, 715, 1072, 1126
RseI CAYNNNNRTG 1 cut(s) 83
SapI GCTCTTC 1 cut(s) 179
SaqAI TTAA 3 cut(s) 819, 1023, 1064
SatI GCNGC 4 cut(s) 606, 609, 677, 1082
Sau3AI GATC 6 cut(s) 178, 349, 364, 519, 573, 600
Sau96I GGNCC 3 cut(s) 52, 471, 493
SchI GAGTC 2 cut(s) 156, 351
ScrFI CCNGG 3 cut(s) 165, 279, 433
SfaNI GCATC 3 cut(s) 286, 784, 1044
SfcI CTRYAG 1 cut(s) 732
SinI GGWCC 1 cut(s) 493
SmiMI CAYNNNNRTG 1 cut(s) 83
Sse9I AATT 4 cut(s) 300, 591, 680, 1087
SsiI CCGC 6 cut(s) 524, 529, 605, 608, 797, 1081
SspMI CTAG 4 cut(s) 144, 360, 450, 957
StyD4I CCNGG 3 cut(s) 163, 277, 431
StyI CCWWGG 2 cut(s) 882, 1129
TaaI ACNGT 6 cut(s) 49, 101, 331, 394, 544, 954
TaiI ACGT 5 cut(s) 403, 638, 654, 765, 941
TaqI TCGA 3 cut(s) 352, 846, 973
TasI AATT 4 cut(s) 300, 591, 680, 1087
TatI WGTACW 1 cut(s) 714
TauI GCSGC 3 cut(s) 608, 611, 1084
TfiI GAWTC 1 cut(s) 962
Tru1I TTAA 3 cut(s) 819, 1023, 1064
Tru9I TTAA 3 cut(s) 819, 1023, 1064
TscAI CASTG 2 cut(s) 54, 62
TseFI GTSAC 2 cut(s) 631, 948
TseI GCWGC 1 cut(s) 676
Tsp45I GTSAC 2 cut(s) 631, 948
TspGWI ACGGA 2 cut(s) 805, 1022
TspRI CASTG 2 cut(s) 54, 62
VpaK11BI GGWCC 1 cut(s) 493
XapI RAATTY 1 cut(s) 1087
XspI CTAG 4 cut(s) 144, 360, 450, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.