Rw7G033150

Domain of unknown function (DUF4149)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0010354
Physical Location & Seq
Reverse (-)
48713822 .. 48717172
3351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G033150.1

Sequence Viewer

Length: 1731 bp
ATGATGAATGTTGTCGCTTTGTTCCTCGTTGTGACTTCACTAGTCAGCGCGGGAATTTGGTCCCCGAGCCCAGATCAACAGAACCAAAAGAAGCAAGGAGAGGAAGTCATAGTGAAAAACGGGCACAGAGTGGTTGTTGTTGAGTACGAGGATCAACAAGGCCACAACCCCAACACCAAGATCTCCATCTCGGCGGATCAAGATGGCCGCCGCAAATCCAGCTCCGCTATTTCCAACAATGACATTTCGACGCCCTCCAAGGTGACGAGGGCCTTGGACAATGCTATGGAGAACATCAAAGAAGCCGCCGCGTCGGTTCTTCCAAATCGAGGGCAGGGACAAGGCCAAGGCCTTTCGAAATCGAAAACTGATGAAGATGGCCACCACTATAGTAGCCCGAAAGAGCTGATATGCGACGCGTATGGCAAATGCAAGCACAAGATTGCCAGTGCTTTGGAGACCAAGAAATATATTTTGAAGGAGACTGCACATGAAGCCAAAGAGACAGCACACAAGGTCATAGAGATCAAGAAGGATATTGACAAGCGGACTGCACATGAAGTCAAGGAGACGGCTCAGAAGGTCGGAGAGGCGGTGGAAGATGCATACGACGCCGTTTCAGAGAGAGCTCAGGAGGTTGGAGAGCGTGCCAAGGAGGCTGCACACAAGGCCAAAGATACAGTAGGAGGTGCATATGGAAGAGCCAAGGATACAGTAGAAGATGCCTATCAGAGAGCCAAAGAGACGGTTTCGGATACAACACATGAGGTTGAAGAACGAGCCAAAGAGTCGTTGGACAAAGCAATGAACACAGCCAAGACAGCAAAGGAAATGGGAAAGACCTTAAGTGGAGACGTACAAAGAAATGTGTACAAATTGAAAACAGCTCCGGAACATGTTAAAGAGAAAGCAAAAGAGGCCAAAGAGACAGTAGAGAATGCAGCAGAAAGAGCCAAAGAGACCGTGGAGCACGCTGCTGATAAGGTTAAGGCCGGGGCAGAGGATATAAAGGATGTGACCGATTGGAGGCTGAATCGAGTGTTTAAGAAAGGAAGAGATATCTTGTATGGTGTGTTAAGGTCTATGGGGTCATTGTTTCCAGCGGTTGATCCTTTGTTATCCGGGCTGAGTTTGTTTGGGTTTGCCACTGCTTATGGTATGTCTATGTGGATAACATTTGTTTCAAGCCATGTGTTGTCTCGGGTGCTTTCCCCGCACCAGTTTGGGATGGTGCAGAGCAAGATTTACAAGGTTTATTTCGCGGCTATGGCCAGTACTGTTGGGATGGCATTGTTGGGCCATCTATTGAACCATTGGAAGAAGTTGACAGGGAGGAATGTTGACGCCTTACAGAACTACAACCTTTTGCTTGCCGAGTTGATGGTTCTGTTCAATATGCTATACTTGGAGCCAAGATCCACTAAGGTGATGTTTGAGAAACTGAAGTTGGAGAAAGAAGAAGGAAGAGGAAGAGAGCATGTTGGTGCCGCAACCAGCACCGAGGTTGAGCGGCATTCACTTACTGCTGAACCCTCAACAACAACTCCCTCAGAAGTCCCAGAAGGGAGGGAGCGTGAGGTAGTTCAGCCTCAGGTTGACAGACCTAACGAGGCACATCAGAGGCTCAAGAAGCTGAATTCCTACTCATCTATCCTCAACATCTTGTCACTAATGGCTCTTACTTGGCATCTAGTATACCTTGCTAAGCGACTGCAAAGTTCTTGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4149

Protein Analysis

576

Amino Acids

63.88

Weight (kDa)

8.62

Isoelectric Point (pI)

31.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4149 PF13664 380 - 480 3.5e-22 Domain of unknown function (DUF4149)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22600 AT1G72100
fragaria_vesca FvH4_5g31970
malus_domestica MD15G1396400.v1.1 MD15G1407700.v1.1
prunus_persica Prupe.1G554000_v2.0.a1
pyrus_communis pycom15g36370
rosa_chinensis RchiOBHm_Chr7g0227991
rosa_laevigata RLG00000001601
rosa_multiflora Rmu_co8508245.1_g000001 Rmu_sc0006493.1_g000005
rosa_roxburghii Rroxscaffold_3G00231680
rosa_rugosa Rorug07G0244900
rosa_samantha Rh7AG398000 Rh7AG398300 Rh7AG398800 Rh7BG380100 Rh7CG416400 Rh7CG416900 Rh7DG394400
rosa_wichuraiana Rw7G033150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1484
AccBSI CCGCTC 1 cut(s) 1510
AccI GTMKAC 1 cut(s) 1695
AccII CGCG 4 cut(s) 50, 311, 419, 1262
AccIII TCCGGA 1 cut(s) 889
AclWI GGATC 4 cut(s) 159, 204, 1103, 1410
AcoI YGGCCR 3 cut(s) 205, 379, 1269
AcsI RAATTY 2 cut(s) 54, 1636
AcuI CTGAAG 1 cut(s) 1463
AcyI GRCGYC 3 cut(s) 251, 612, 1344
AdeI CACNNNGTG 1 cut(s) 130
AfaI GTAC 4 cut(s) 146, 858, 872, 1276
AfiI CCNNNNNNNGG 2 cut(s) 329, 1026
AflII CTTAAG 1 cut(s) 844
AflIII ACRYGT 2 cut(s) 417, 895
AgsI TTSAA 6 cut(s) 478, 773, 880, 1185, 1309, 1393
AhlI ACTAGT 1 cut(s) 40
AjuI GAANNNNNNNTTGG 2 cut(s) 1430, 1462
AleI CACNNNNGTG 1 cut(s) 1424
AluBI AGCT 5 cut(s) 222, 406, 629, 887, 1633
AluI AGCT 5 cut(s) 222, 406, 629, 887, 1633
Alw21I GWGCWC 2 cut(s) 631, 972
Alw26I GTCTC 9 cut(s) 452, 476, 497, 563, 737, 846, 920, 953, 1203
AlwI GGATC 4 cut(s) 159, 204, 1103, 1410
Ama87I CYCGRG 2 cut(s) 64, 1200
Aor13HI TCCGGA 1 cut(s) 889
ApeKI GCWGC 4 cut(s) 659, 941, 974, 1725
ApoI RAATTY 2 cut(s) 54, 1636
AspLEI GCGC 1 cut(s) 50
AspS9I GGNCC 3 cut(s) 60, 270, 1297
AsuC2I CCSGG 2 cut(s) 994, 1123
AsuHPI GGTGA 2 cut(s) 274, 1438
AsuII TTCGAA 1 cut(s) 356
AvaI CYCGRG 2 cut(s) 64, 1200
AvaII GGWCC 1 cut(s) 60
AxyI CCTNAGG 1 cut(s) 1590
BaeGI GKGCMC 1 cut(s) 126
BalI TGGCCA 2 cut(s) 381, 1271
BanI GGYRCC 1 cut(s) 1484
BanII GRGCYC 2 cut(s) 71, 631
Bbv12I GWGCWC 2 cut(s) 631, 972
BbvI GCAGC 4 cut(s) 646, 953, 961, 1712
BccI CCATC 7 cut(s) 194, 197, 371, 1222, 1279, 1308, 1375
BceAI ACGGC 2 cut(s) 588, 599
BciVI GTATCC 2 cut(s) 703, 748
BcnI CCSGG 2 cut(s) 994, 1123
BcoDI GTCTC 9 cut(s) 452, 476, 497, 563, 737, 846, 920, 953, 1203
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 2 cut(s) 41, 1691
BfmI CTRYAG 1 cut(s) 388
BfrI CTTAAG 1 cut(s) 844
BfuI GTATCC 2 cut(s) 703, 748
BglI GCCNNNNNGGC 1 cut(s) 656
BglII AGATCT 1 cut(s) 180
BlpI GCTNAGC 1 cut(s) 1704
BmcAI AGTACT 1 cut(s) 1276
Bme1390I CCNGG 2 cut(s) 994, 1123
Bme18I GGWCC 1 cut(s) 60
BmeT110I CYCGRG 2 cut(s) 64, 1200
BmgT120I GGNCC 3 cut(s) 60, 270, 1297
BmiI GGNNCC 3 cut(s) 62, 1410, 1486
BmrFI CCNGG 2 cut(s) 994, 1123
BmsI GCATC 3 cut(s) 592, 712, 1696
Bpu10I CCTNAGC 1 cut(s) 630
Bpu1102I GCTNAGC 1 cut(s) 1704
Bpu14I TTCGAA 1 cut(s) 356
BpuEI CTTGAG 1 cut(s) 1610
BpuMI CCSGG 2 cut(s) 994, 1123
BsaBI GATNNNNATC 2 cut(s) 185, 726
BsaHI GRCGYC 3 cut(s) 251, 612, 1344
BsaI GGTCTC 2 cut(s) 452, 953
BsaJI CCNNGG 8 cut(s) 258, 273, 346, 651, 705, 963, 993, 1500
BsaWI WCCGGW 1 cut(s) 889
BsaXI ACNNNNNCTCC 8 cut(s) 90, 120, 579, 609, 1528, 1558, 1558, 1588
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 1026
Bse1I ACTGG 3 cut(s) 447, 1219, 1272
Bse21I CCTNAGG 1 cut(s) 1590
Bse3DI GCAATG 1 cut(s) 810
Bse8I GATNNNNATC 2 cut(s) 185, 726
BseAI TCCGGA 1 cut(s) 889
BseDI CCNNGG 8 cut(s) 258, 273, 346, 651, 705, 963, 993, 1500
BseGI GGATG 3 cut(s) 1018, 1233, 1290
BseJI GATNNNNATC 2 cut(s) 185, 726
BseLI CCNNNNNNNGG 2 cut(s) 329, 1026
BseMI GCAATG 1 cut(s) 810
BseMII CTCAG 5 cut(s) 590, 644, 1118, 1563, 1604
BseNI ACTGG 3 cut(s) 447, 1219, 1272
BseSI GKGCMC 1 cut(s) 126
BseXI GCAGC 4 cut(s) 646, 953, 961, 1712
BsgI GTGCAG 4 cut(s) 471, 537, 645, 1253
Bsh1236I CGCG 4 cut(s) 50, 311, 419, 1262
BshNI GGYRCC 1 cut(s) 1484
BsiHKAI GWGCWC 2 cut(s) 631, 972
BsiHKCI CYCGRG 2 cut(s) 64, 1200
BsiSI CCGG 3 cut(s) 890, 993, 1122
BslFI GGGAC 3 cut(s) 46, 351, 1541
BslI CCNNNNNNNGG 2 cut(s) 329, 1026
BsmAI GTCTC 9 cut(s) 452, 476, 497, 563, 737, 846, 920, 953, 1203
BsmBI CGTCTC 3 cut(s) 563, 737, 846
BsmFI GGGAC 3 cut(s) 46, 351, 1541
BsmI GAATGC 2 cut(s) 943, 1513
Bso31I GGTCTC 2 cut(s) 452, 953
BsoBI CYCGRG 2 cut(s) 64, 1200
Bsp119I TTCGAA 1 cut(s) 356
Bsp1286I GDGCHC 4 cut(s) 71, 126, 631, 972
Bsp13I TCCGGA 1 cut(s) 889
Bsp1407I TGTACA 1 cut(s) 870
Bsp143I GATC 7 cut(s) 73, 151, 180, 196, 525, 1108, 1415
Bsp1720I GCTNAGC 1 cut(s) 1704
BspCNI CTCAG 5 cut(s) 589, 643, 1119, 1562, 1603
BspEI TCCGGA 1 cut(s) 889
BspFNI CGCG 4 cut(s) 50, 311, 419, 1262
BspLI GGNNCC 3 cut(s) 62, 1410, 1486
BspPI GGATC 4 cut(s) 159, 204, 1103, 1410
BspQI GCTCTTC 1 cut(s) 694
BspT104I TTCGAA 1 cut(s) 356
BspT107I GGYRCC 1 cut(s) 1484
BspTI CTTAAG 1 cut(s) 844
BspTNI GGTCTC 2 cut(s) 452, 953
BsrBI CCGCTC 1 cut(s) 1510
BsrDI GCAATG 1 cut(s) 810
BsrGI TGTACA 1 cut(s) 870
BsrI ACTGG 3 cut(s) 447, 1219, 1272
BssECI CCNNGG 8 cut(s) 258, 273, 346, 651, 705, 963, 993, 1500
BssMI GATC 7 cut(s) 73, 151, 180, 196, 525, 1108, 1415
BssNAI GTATAC 1 cut(s) 1696
BssNI GRCGYC 3 cut(s) 251, 612, 1344
BssT1I CCWWGG 5 cut(s) 258, 273, 346, 651, 705
Bst1107I GTATAC 1 cut(s) 1696
Bst4CI ACNGT 6 cut(s) 682, 715, 748, 931, 964, 1279
Bst6I CTCTTC 4 cut(s) 694, 1048, 1459, 1465
BstACI GRCGYC 3 cut(s) 251, 612, 1344
BstAFI CTTAAG 1 cut(s) 844
BstAUI TGTACA 1 cut(s) 870
BstBI TTCGAA 1 cut(s) 356
BstC8I GCNNGC 4 cut(s) 434, 648, 972, 1371
BstDEI CTNAG 7 cut(s) 576, 630, 1127, 1422, 1549, 1590, 1704
BstDSI CCRYGG 1 cut(s) 963
BstF5I GGATG 3 cut(s) 1018, 1233, 1290
BstFNI CGCG 4 cut(s) 50, 311, 419, 1262
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 7 cut(s) 76, 154, 183, 199, 528, 1111, 1418
BstMAI GTCTC 9 cut(s) 452, 476, 497, 563, 737, 846, 920, 953, 1203
BstMBI GATC 7 cut(s) 73, 151, 180, 196, 525, 1108, 1415
BstNSI RCATGY 2 cut(s) 899, 1481
BstSCI CCNGG 2 cut(s) 992, 1121
BstSFI CTRYAG 1 cut(s) 388
BstSLI GKGCMC 1 cut(s) 126
BstUI CGCG 4 cut(s) 50, 311, 419, 1262
BstV1I GCAGC 4 cut(s) 646, 953, 961, 1712
BstX2I RGATCY 2 cut(s) 180, 1415
BstXI CCANNNNNNTGG 1 cut(s) 454
BstYI RGATCY 2 cut(s) 180, 1415
BstZ17I GTATAC 1 cut(s) 1696
Bsu36I CCTNAGG 1 cut(s) 1590
BsuI GTATCC 2 cut(s) 703, 748
BtgI CCRYGG 1 cut(s) 963
BtsCI GGATG 3 cut(s) 1018, 1233, 1290
BtsI GCAGTG 1 cut(s) 1146
BtsIMutI CAGTG 2 cut(s) 454, 1146
Cac8I GCNNGC 4 cut(s) 434, 648, 972, 1371
CfoI GCGC 1 cut(s) 50
Cfr13I GGNCC 3 cut(s) 60, 270, 1297
CseI GACGC 5 cut(s) 259, 300, 425, 620, 1352
Csp6I GTAC 4 cut(s) 145, 857, 871, 1275
CviAII CATG 6 cut(s) 491, 557, 764, 896, 1190, 1478
CviQI GTAC 4 cut(s) 145, 857, 871, 1275
DdeI CTNAG 7 cut(s) 576, 630, 1127, 1422, 1549, 1590, 1704
DpnI GATC 7 cut(s) 75, 153, 182, 198, 527, 1110, 1417
DpnII GATC 7 cut(s) 73, 151, 180, 196, 525, 1108, 1415
DraIII CACNNNGTG 1 cut(s) 130
EaeI YGGCCR 3 cut(s) 205, 379, 1269
Eam1104I CTCTTC 4 cut(s) 694, 1048, 1459, 1465
EarI CTCTTC 4 cut(s) 694, 1048, 1459, 1465
EciI GGCGGA 1 cut(s) 209
Ecl136II GAGCTC 1 cut(s) 629
Eco130I CCWWGG 5 cut(s) 258, 273, 346, 651, 705
Eco147I AGGCCT 1 cut(s) 351
Eco24I GRGCYC 2 cut(s) 71, 631
Eco31I GGTCTC 2 cut(s) 452, 953
Eco32I GATATC 1 cut(s) 1060
Eco47I GGWCC 1 cut(s) 60
Eco53kI GAGCTC 1 cut(s) 629
Eco57I CTGAAG 1 cut(s) 1463
Eco81I CCTNAGG 1 cut(s) 1590
Eco88I CYCGRG 2 cut(s) 64, 1200
EcoICRI GAGCTC 1 cut(s) 629
EcoO109I RGGNCCY 1 cut(s) 270
EcoRI GAATTC 1 cut(s) 1636
EcoRV GATATC 1 cut(s) 1060
EcoT14I CCWWGG 5 cut(s) 258, 273, 346, 651, 705
EcoT22I ATGCAT 1 cut(s) 607
EcoT38I GRGCYC 2 cut(s) 71, 631
ErhI CCWWGG 5 cut(s) 258, 273, 346, 651, 705
Esp3I CGTCTC 3 cut(s) 563, 737, 846
FaeI CATG 6 cut(s) 494, 560, 767, 899, 1193, 1481
FaqI GGGAC 3 cut(s) 46, 351, 1541
FatI CATG 6 cut(s) 490, 556, 763, 895, 1189, 1477
FauI CCCGC 2 cut(s) 43, 1221
FauNDI CATATG 1 cut(s) 694
FblI GTMKAC 1 cut(s) 1695
FokI GGATG 3 cut(s) 1025, 1240, 1297
FriOI GRGCYC 2 cut(s) 71, 631
FspBI CTAG 2 cut(s) 41, 1691
GlaI GCGC 1 cut(s) 49
HapII CCGG 3 cut(s) 890, 993, 1122
HgaI GACGC 5 cut(s) 259, 300, 425, 620, 1352
HhaI GCGC 1 cut(s) 50
Hin1I GRCGYC 3 cut(s) 251, 612, 1344
Hin1II CATG 6 cut(s) 494, 560, 767, 899, 1193, 1481
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HincII GTYRAC 3 cut(s) 1326, 1342, 1597
HindII GTYRAC 3 cut(s) 1326, 1342, 1597
HinfI GANTC 2 cut(s) 788, 1033
HpaII CCGG 3 cut(s) 890, 993, 1122
HphI GGTGA 2 cut(s) 274, 1438
Hpy166II GTNNAC 5 cut(s) 871, 1326, 1342, 1597, 1696
Hpy188I TCNGA 7 cut(s) 579, 587, 622, 732, 754, 1552, 1620
Hpy188III TCNNGA 5 cut(s) 200, 529, 632, 890, 1627
Hpy8I GTNNAC 5 cut(s) 871, 1326, 1342, 1597, 1696
Hpy99I CGWCG 4 cut(s) 253, 316, 419, 614
HpyAV CCTTC 5 cut(s) 472, 526, 574, 1454, 1556
HpyCH4III ACNGT 6 cut(s) 682, 715, 748, 931, 964, 1279
HpyCH4IV ACGT 1 cut(s) 855
HpyCH4V TGCA 9 cut(s) 432, 488, 554, 605, 662, 692, 941, 1234, 1714
HpyF3I CTNAG 7 cut(s) 576, 630, 1127, 1422, 1549, 1590, 1704
HpySE526I ACGT 1 cut(s) 855
Hsp92I GRCGYC 3 cut(s) 251, 612, 1344
Hsp92II CATG 6 cut(s) 494, 560, 767, 899, 1193, 1481
HspAI GCGC 1 cut(s) 48
Kpn2I TCCGGA 1 cut(s) 889
Kzo9I GATC 7 cut(s) 73, 151, 180, 196, 525, 1108, 1415
LguI GCTCTTC 1 cut(s) 694
LmnI GCTCC 5 cut(s) 227, 892, 967, 1408, 1570
Lsp1109I GCAGC 4 cut(s) 646, 953, 961, 1712
LweI GCATC 3 cut(s) 592, 712, 1696
MaeI CTAG 2 cut(s) 41, 1691
MaeII ACGT 1 cut(s) 855
MaeIII GTNAC 4 cut(s) 31, 262, 1015, 1665
MalI GATC 7 cut(s) 75, 153, 182, 198, 527, 1110, 1417
MbiI CCGCTC 1 cut(s) 1510
MboI GATC 7 cut(s) 73, 151, 180, 196, 525, 1108, 1415
MflI RGATCY 2 cut(s) 180, 1415
MhlI GDGCHC 4 cut(s) 71, 126, 631, 972
MlsI TGGCCA 2 cut(s) 381, 1271
MluCI AATT 3 cut(s) 54, 875, 1636
MluI ACGCGT 1 cut(s) 417
MluNI TGGCCA 2 cut(s) 381, 1271
MlyI GAGTC 1 cut(s) 797
MmeI TCCRAC 5 cut(s) 258, 565, 619, 774, 1428
Mox20I TGGCCA 2 cut(s) 381, 1271
Mph1103I ATGCAT 1 cut(s) 607
MroI TCCGGA 1 cut(s) 889
MscI TGGCCA 2 cut(s) 381, 1271
MseI TTAA 5 cut(s) 845, 900, 987, 1044, 1076
MslI CAYNNNNRTG 2 cut(s) 1424, 1482
Msp20I TGGCCA 2 cut(s) 381, 1271
MspA1I CMGCKG 1 cut(s) 1103
MspCI CTTAAG 1 cut(s) 844
MspI CCGG 3 cut(s) 890, 993, 1122
MspR9I CCNGG 2 cut(s) 994, 1123
Mva1269I GAATGC 2 cut(s) 943, 1513
MvnI CGCG 4 cut(s) 50, 311, 419, 1262
NciI CCSGG 2 cut(s) 994, 1123
NdeI CATATG 1 cut(s) 694
NdeII GATC 7 cut(s) 73, 151, 180, 196, 525, 1108, 1415
NlaIII CATG 6 cut(s) 494, 560, 767, 899, 1193, 1481
NlaIV GGNNCC 3 cut(s) 62, 1410, 1486
NmeAIII GCCGAG 2 cut(s) 170, 1399
NmuCI GTSAC 4 cut(s) 31, 262, 1015, 1665
NsiI ATGCAT 1 cut(s) 607
NspI RCATGY 2 cut(s) 899, 1481
NspV TTCGAA 1 cut(s) 356
OliI CACNNNNGTG 1 cut(s) 1424
PceI AGGCCT 1 cut(s) 351
PciI ACATGT 1 cut(s) 895
PciSI GCTCTTC 1 cut(s) 694
PctI GAATGC 2 cut(s) 943, 1513
PfeI GAWTC 1 cut(s) 1033
PleI GAGTC 1 cut(s) 796
PpsI GAGTC 1 cut(s) 796
PscI ACATGT 1 cut(s) 895
Psp124BI GAGCTC 1 cut(s) 631
PspN4I GGNNCC 3 cut(s) 62, 1410, 1486
PspPI GGNCC 3 cut(s) 60, 270, 1297
PsuI RGATCY 2 cut(s) 180, 1415
RsaI GTAC 4 cut(s) 146, 858, 872, 1276
RsaNI GTAC 4 cut(s) 145, 857, 871, 1275
RseI CAYNNNNRTG 2 cut(s) 1424, 1482
SacI GAGCTC 1 cut(s) 631
SapI GCTCTTC 1 cut(s) 694
SaqAI TTAA 5 cut(s) 845, 900, 987, 1044, 1076
Sau3AI GATC 7 cut(s) 73, 151, 180, 196, 525, 1108, 1415
Sau96I GGNCC 3 cut(s) 60, 270, 1297
ScaI AGTACT 1 cut(s) 1276
SchI GAGTC 1 cut(s) 797
ScrFI CCNGG 2 cut(s) 994, 1123
SduI GDGCHC 4 cut(s) 71, 126, 631, 972
SfaNI GCATC 3 cut(s) 592, 712, 1696
SfcI CTRYAG 1 cut(s) 388
SfuI TTCGAA 1 cut(s) 356
SinI GGWCC 1 cut(s) 60
SmiMI CAYNNNNRTG 2 cut(s) 1424, 1482
SmlI CTYRAG 2 cut(s) 844, 1625
SmoI CTYRAG 2 cut(s) 844, 1625
SpeI ACTAGT 1 cut(s) 40
Sse9I AATT 3 cut(s) 54, 875, 1636
SseBI AGGCCT 1 cut(s) 351
SspMI CTAG 2 cut(s) 41, 1691
SstI GAGCTC 1 cut(s) 631
StuI AGGCCT 1 cut(s) 351
StyD4I CCNGG 2 cut(s) 992, 1121
StyI CCWWGG 5 cut(s) 258, 273, 346, 651, 705
TaaI ACNGT 6 cut(s) 682, 715, 748, 931, 964, 1279
TaiI ACGT 1 cut(s) 858
TaqI TCGA 5 cut(s) 248, 328, 356, 362, 1036
TaqII GACCGA 1 cut(s) 1034
TasI AATT 3 cut(s) 54, 875, 1636
TatI WGTACW 2 cut(s) 870, 1274
TauI GCSGC 7 cut(s) 210, 213, 308, 311, 1265, 1490, 1513
TfiI GAWTC 1 cut(s) 1033
Tru1I TTAA 5 cut(s) 845, 900, 987, 1044, 1076
Tru9I TTAA 5 cut(s) 845, 900, 987, 1044, 1076
TscAI CASTG 2 cut(s) 454, 1153
TseFI GTSAC 4 cut(s) 31, 262, 1015, 1665
TseI GCWGC 4 cut(s) 659, 941, 974, 1725
Tsp45I GTSAC 4 cut(s) 31, 262, 1015, 1665
TspDTI ATGAA 5 cut(s) 20, 387, 507, 573, 821
TspRI CASTG 2 cut(s) 454, 1153
Vha464I CTTAAG 1 cut(s) 844
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 2 cut(s) 54, 1636
XceI RCATGY 2 cut(s) 899, 1481
XcmI CCANNNNNNNNNTGG 2 cut(s) 790, 961
XmiI GTMKAC 1 cut(s) 1695
XspI CTAG 2 cut(s) 41, 1691
ZrmI AGTACT 1 cut(s) 1276
Zsp2I ATGCAT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.