MD15G1407700.v1.1

Domain of unknown function (DUF4149)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010354
Physical Location & Seq
Reverse (-)
50782029 .. 50784586
2558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1407700.v1.1.491

Sequence Viewer

Length: 1584 bp
ATGATGAACTTTGTGGCTCTGTTCCTTGTTGTGACTTCACTGGTAACCGCTGGGGTGTGGTTTCCGTCTGCCAGCCCACCAAGTGATCAGACCCAGCAGCAGAAGCAGAGGCAAGAAGGTCCTCAGCAGCAGCATGCTAGGGATCAGGAAGTCATTGTCAAAAATGGGCACAGGGTGGTTGTCGTGGAGTACGATGAGCATGGCCACCACAAAACCAAAGTCTCAATCTCGCCGGAACACGGCCATCCACCCCGCAGAGAGTCTTCTTCCTTCTCCGAAAATGAAGTATCGTCGCCCTCGAAGATTTCTAGGGCTTTCGACAACTCCATGGAAAACATCAAAGAAGCGGCCTCTGGTCTCGCTAACTTTGGCCAAGGCCATGGCGAGGGTCACAGTCCGAAAGAGCTCATGTGCGATGCCTACGGAAGATGCAAGCACAACATTGCAAGTGCTATCGAGACCAAAAAGGAGATTTGTAAGGAGACAGCACACGGGGCAAAGGAGGCGGCGCATAAGGTAGGTGAGGCGGCACACAGGGCAGGCGAGGCGGTCGAAAATGTGTACGAGAGGGCGAAAGATACGGTGTCGGATAAGGCGCACGAGGTTGAGGAAACTGCTAGAGACCCAATGGAAAAAGCAAAGAACGCCTCCAAGACGGCCAAGGACATGGGGGACACGTTGGCGGAAAAAGCAAAAGAAGCTAAAGAGACCGCGGAGGAAGCAGCTTTTAAGGTGAAGGCTGGAGCGGAGGAGCTCAAAAGTGACTTGGCTGAAAAAGCGAAAGAAGCGAAAGAGACTGCGGAGGAAGCAGCTGGTAAGGTTAAGGCTGGAGCGGAGGAGCTCAAAAGTGGGACCCAGAAGAGGCTGCATCAGTCGAGGGACAAAGTAAGAGAGTTCTGGTACGGTGCATTCAGGTCCATAGCATCGGGTGACTTACTGGAGGGTTTGATGTCCGTGGCGCATCTATTCGGGCTGGCTACGGCTTACGGAATGTGCATGTGGATTACGTTCGTGTCGAGCCACGTGTTGGCGTGGAGTCTGCCGCCGCAGCAGTTTGGGATGGTACAGAGCAAGATTTACAAGGTTTATTTCAAGGCCATGGCGTATAGTGTTGGGATGGCATTGTTGGGTCATCTGTGGAGACATAGAAGCTCATTGTTTCGAGGGAAGGCGGCGGATATGTTGCAGAACTATAATCTTATCGCTGCTCTTTCCATGATCTTATTCAATATGATGTACTTGGAGCCTCGATCCACTAAGGTGATGTTTGAGAGAATGAAGATCGAGAAGGAAGAAGGAAGAGGAAGAGAGAGAGTACATGTTGATGCCAGAATCAGCAGGGAGGCTGGCCAGCAGCAGCAGCAGCAGACTACCACTGAAGGCGAACCCTCAATCACGACGCCTCCACAAGTCTCGAGGAGGGAGCAACAGGTGGTTGAGCCTGATATTGAGAGGCTTAACGAGATGCGTGAGAGCCTGAAGAAGCTAAATACATGTTCGTCGTTCCTCAACATCGCGTCACTAATGGCTCTTAGCTGGCATCTTGTGTATCTTGCCCAGCACCTCCCCAGTTCATGCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4149

Protein Analysis

528

Amino Acids

58.7

Weight (kDa)

7.35

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4149 PF13664 324 - 426 5e-23 Domain of unknown function (DUF4149)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22600 AT1G72100
fragaria_vesca FvH4_5g31970
malus_domestica MD15G1396400.v1.1 MD15G1407700.v1.1
prunus_persica Prupe.1G554000_v2.0.a1
pyrus_communis pycom15g36370
rosa_chinensis RchiOBHm_Chr7g0227991
rosa_laevigata RLG00000001601
rosa_multiflora Rmu_co8508245.1_g000001 Rmu_sc0006493.1_g000005
rosa_roxburghii Rroxscaffold_3G00231680
rosa_rugosa Rorug07G0244900
rosa_samantha Rh7AG398000 Rh7AG398300 Rh7AG398800 Rh7BG380100 Rh7CG416400 Rh7CG416900 Rh7DG394400
rosa_wichuraiana Rw7G033150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1027
AccBSI CCGCTC 2 cut(s) 746, 833
AccII CGCG 2 cut(s) 713, 1517
AclWI GGATC 2 cut(s) 150, 1245
AcoI YGGCCR 5 cut(s) 202, 241, 370, 657, 1348
AcuI CTGAAG 2 cut(s) 1398, 1499
AcvI CACGTG 1 cut(s) 1024
AcyI GRCGYC 1 cut(s) 1400
AdeI CACNNNGTG 2 cut(s) 83, 175
AfaI GTAC 6 cut(s) 191, 563, 902, 1065, 1238, 1317
AfiI CCNNNNNNNGG 4 cut(s) 239, 385, 861, 1027
AflIII ACRYGT 4 cut(s) 675, 1023, 1318, 1493
AgsI TTSAA 2 cut(s) 1093, 1228
AleI CACNNNNGTG 1 cut(s) 1259
AluBI AGCT 9 cut(s) 406, 701, 725, 754, 812, 841, 1152, 1486, 1536
AluI AGCT 9 cut(s) 406, 701, 725, 754, 812, 841, 1152, 1486, 1536
Alw21I GWGCWC 3 cut(s) 408, 756, 843
Alw26I GTCTC 9 cut(s) 226, 362, 452, 476, 615, 701, 788, 1135, 1417
AlwI GGATC 2 cut(s) 150, 1245
Ama87I CYCGRG 1 cut(s) 1414
AoxI GGCC 8 cut(s) 202, 241, 348, 370, 376, 657, 1095, 1348
ArsI GACNNNNNNTTYG 2 cut(s) 204, 236
AspLEI GCGC 3 cut(s) 511, 598, 961
AspS9I GGNCC 3 cut(s) 119, 852, 915
AsuHPI GGTGA 4 cut(s) 533, 745, 941, 1273
AvaI CYCGRG 1 cut(s) 1414
AvaII GGWCC 3 cut(s) 119, 852, 915
BaeGI GKGCMC 1 cut(s) 171
BalI TGGCCA 3 cut(s) 204, 372, 1350
BanII GRGCYC 3 cut(s) 408, 756, 843
BauI CACGAG 1 cut(s) 599
BbrPI CACGTG 1 cut(s) 1024
BbsI GAAGAC 1 cut(s) 255
Bbv12I GWGCWC 3 cut(s) 408, 756, 843
BbvCI CCTCAGC 1 cut(s) 123
BccI CCATC 3 cut(s) 252, 1054, 1111
BceAI ACGGC 3 cut(s) 256, 672, 996
BclI TGATCA 1 cut(s) 85
BcoDI GTCTC 9 cut(s) 226, 362, 452, 476, 615, 701, 788, 1135, 1417
BfaI CTAG 3 cut(s) 138, 309, 618
Bme18I GGWCC 3 cut(s) 119, 852, 915
BmeT110I CYCGRG 1 cut(s) 1414
BmgT120I GGNCC 3 cut(s) 119, 852, 915
BmiI GGNNCC 3 cut(s) 853, 854, 1245
BmrI ACTGGG 1 cut(s) 1563
BmsI GCATC 8 cut(s) 406, 419, 877, 932, 970, 1315, 1455, 1549
BmuI ACTGGG 1 cut(s) 1563
BpiI GAAGAC 1 cut(s) 255
BpmI CTGGAG 3 cut(s) 762, 849, 959
Bpu10I CCTNAGC 1 cut(s) 123
BsaAI YACGTR 1 cut(s) 1024
BsaHI GRCGYC 1 cut(s) 1400
BsaI GGTCTC 4 cut(s) 362, 452, 615, 701
BsaJI CCNNGG 7 cut(s) 327, 373, 379, 660, 711, 954, 1098
BsaXI ACNNNNNCTCC 2 cut(s) 1387, 1417
Bsc4I CCNNNNNNNGG 4 cut(s) 239, 385, 861, 1027
Bse1I ACTGG 3 cut(s) 45, 942, 1569
Bse3DI GCAATG 1 cut(s) 441
BseDI CCNNGG 7 cut(s) 327, 373, 379, 660, 711, 954, 1098
BseGI GGATG 3 cut(s) 244, 1065, 1122
BseLI CCNNNNNNNGG 4 cut(s) 239, 385, 861, 1027
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 1 cut(s) 137
BseNI ACTGG 3 cut(s) 45, 942, 1569
BseRI GAGGAG 3 cut(s) 764, 851, 1432
BseSI GKGCMC 1 cut(s) 171
BseYI CCCAGC 3 cut(s) 50, 93, 1557
Bsh1236I CGCG 2 cut(s) 713, 1517
Bsh1285I CGRYCG 1 cut(s) 552
BshFI GGCC 8 cut(s) 204, 243, 350, 372, 378, 659, 1097, 1350
BsiEI CGRYCG 1 cut(s) 552
BsiHKAI GWGCWC 3 cut(s) 408, 756, 843
BsiHKCI CYCGRG 1 cut(s) 1414
BsiSI CCGG 1 cut(s) 233
BslFI GGGAC 3 cut(s) 686, 865, 893
BslI CCNNNNNNNGG 4 cut(s) 239, 385, 861, 1027
BsmAI GTCTC 9 cut(s) 226, 362, 452, 476, 615, 701, 788, 1135, 1417
BsmFI GGGAC 3 cut(s) 686, 865, 893
BsmI GAATGC 1 cut(s) 908
BsnI GGCC 8 cut(s) 204, 243, 350, 372, 378, 659, 1097, 1350
Bso31I GGTCTC 4 cut(s) 362, 452, 615, 701
BsoBI CYCGRG 1 cut(s) 1414
Bsp1286I GDGCHC 4 cut(s) 171, 408, 756, 843
Bsp143I GATC 5 cut(s) 85, 142, 1218, 1250, 1281
Bsp19I CCATGG 3 cut(s) 327, 379, 1098
BspANI GGCC 8 cut(s) 204, 243, 350, 372, 378, 659, 1097, 1350
BspCNI CTCAG 1 cut(s) 136
BspFNI CGCG 2 cut(s) 713, 1517
BspLI GGNNCC 3 cut(s) 853, 854, 1245
BspPI GGATC 2 cut(s) 150, 1245
BspTNI GGTCTC 4 cut(s) 362, 452, 615, 701
BsrBI CCGCTC 2 cut(s) 746, 833
BsrDI GCAATG 1 cut(s) 441
BsrI ACTGG 3 cut(s) 45, 942, 1569
BssECI CCNNGG 7 cut(s) 327, 373, 379, 660, 711, 954, 1098
BssMI GATC 5 cut(s) 85, 142, 1218, 1250, 1281
BssNI GRCGYC 1 cut(s) 1400
BssSI CACGAG 1 cut(s) 599
BssT1I CCWWGG 5 cut(s) 327, 373, 379, 660, 1098
Bst2BI CACGAG 1 cut(s) 599
Bst4CI ACNGT 3 cut(s) 395, 583, 905
Bst6I CTCTTC 3 cut(s) 854, 1294, 1300
BstACI GRCGYC 1 cut(s) 1400
BstBAI YACGTR 1 cut(s) 1024
BstC8I GCNNGC 8 cut(s) 73, 135, 434, 541, 975, 1348, 1352, 1538
BstDEI CTNAG 3 cut(s) 123, 1257, 1532
BstDSI CCRYGG 5 cut(s) 327, 379, 711, 954, 1098
BstEII GGTNACC 1 cut(s) 43
BstF5I GGATG 3 cut(s) 244, 1065, 1122
BstFNI CGCG 2 cut(s) 713, 1517
BstHHI GCGC 3 cut(s) 511, 598, 961
BstKTI GATC 5 cut(s) 88, 145, 1221, 1253, 1284
BstMAI GTCTC 9 cut(s) 226, 362, 452, 476, 615, 701, 788, 1135, 1417
BstMBI GATC 5 cut(s) 85, 142, 1218, 1250, 1281
BstMCI CGRYCG 1 cut(s) 552
BstNSI RCATGY 4 cut(s) 137, 1000, 1322, 1497
BstPI GGTNACC 1 cut(s) 43
BstSLI GKGCMC 1 cut(s) 171
BstUI CGCG 2 cut(s) 713, 1517
BstV2I GAAGAC 1 cut(s) 255
BstXI CCANNNNNNTGG 2 cut(s) 380, 667
BsuRI GGCC 8 cut(s) 204, 243, 350, 372, 378, 659, 1097, 1350
BtgI CCRYGG 5 cut(s) 327, 379, 711, 954, 1098
BtgZI GCGATG 2 cut(s) 429, 1498
BtsCI GGATG 3 cut(s) 244, 1065, 1122
BtsIMutI CAGTG 2 cut(s) 38, 1374
Cac8I GCNNGC 8 cut(s) 73, 135, 434, 541, 975, 1348, 1352, 1538
CfoI GCGC 3 cut(s) 511, 598, 961
Cfr13I GGNCC 3 cut(s) 119, 852, 915
Cfr42I CCGCGG 1 cut(s) 714
CseI GACGC 2 cut(s) 1408, 1506
Csp6I GTAC 6 cut(s) 190, 562, 901, 1064, 1237, 1316
CviQI GTAC 6 cut(s) 190, 562, 901, 1064, 1237, 1316
DdeI CTNAG 3 cut(s) 123, 1257, 1532
DpnI GATC 5 cut(s) 87, 144, 1220, 1252, 1283
DpnII GATC 5 cut(s) 85, 142, 1218, 1250, 1281
DraIII CACNNNGTG 2 cut(s) 83, 175
EaeI YGGCCR 5 cut(s) 202, 241, 370, 657, 1348
Eam1104I CTCTTC 3 cut(s) 854, 1294, 1300
EarI CTCTTC 3 cut(s) 854, 1294, 1300
EciI GGCGGA 2 cut(s) 698, 1190
Ecl136II GAGCTC 3 cut(s) 406, 754, 841
Eco130I CCWWGG 5 cut(s) 327, 373, 379, 660, 1098
Eco24I GRGCYC 3 cut(s) 408, 756, 843
Eco31I GGTCTC 4 cut(s) 362, 452, 615, 701
Eco47I GGWCC 3 cut(s) 119, 852, 915
Eco53kI GAGCTC 3 cut(s) 406, 754, 841
Eco57I CTGAAG 2 cut(s) 1398, 1499
Eco72I CACGTG 1 cut(s) 1024
Eco88I CYCGRG 1 cut(s) 1414
Eco91I GGTNACC 1 cut(s) 43
EcoICRI GAGCTC 3 cut(s) 406, 754, 841
EcoO109I RGGNCCY 2 cut(s) 119, 852
EcoO65I GGTNACC 1 cut(s) 43
EcoT14I CCWWGG 5 cut(s) 327, 373, 379, 660, 1098
EcoT38I GRGCYC 3 cut(s) 408, 756, 843
ErhI CCWWGG 5 cut(s) 327, 373, 379, 660, 1098
FaqI GGGAC 3 cut(s) 686, 865, 893
FauI CCCGC 1 cut(s) 260
FbaI TGATCA 1 cut(s) 85
FokI GGATG 3 cut(s) 231, 1072, 1129
FriOI GRGCYC 3 cut(s) 408, 756, 843
FspBI CTAG 3 cut(s) 138, 309, 618
GlaI GCGC 3 cut(s) 510, 597, 960
GsaI CCCAGC 3 cut(s) 54, 97, 1561
GsuI CTGGAG 3 cut(s) 762, 849, 959
HaeIII GGCC 8 cut(s) 204, 243, 350, 372, 378, 659, 1097, 1350
HapII CCGG 1 cut(s) 233
HgaI GACGC 2 cut(s) 1408, 1506
HhaI GCGC 3 cut(s) 511, 598, 961
Hin1I GRCGYC 1 cut(s) 1400
Hin6I GCGC 3 cut(s) 509, 596, 959
HinP1I GCGC 3 cut(s) 509, 596, 959
HinfI GANTC 3 cut(s) 260, 1036, 1332
HpaII CCGG 1 cut(s) 233
HphI GGTGA 4 cut(s) 533, 745, 941, 1273
Hpy166II GTNNAC 1 cut(s) 562
Hpy188I TCNGA 4 cut(s) 90, 277, 399, 589
Hpy188III TCNNGA 5 cut(s) 146, 457, 1285, 1396, 1414
Hpy8I GTNNAC 1 cut(s) 562
Hpy99I CGWCG 3 cut(s) 295, 1402, 1504
HpyAV CCTTC 7 cut(s) 110, 280, 730, 1162, 1282, 1289, 1373
HpyCH4III ACNGT 3 cut(s) 395, 583, 905
HpyCH4IV ACGT 3 cut(s) 677, 1007, 1023
HpyCH4V TGCA 6 cut(s) 432, 446, 868, 908, 996, 1186
HpyF3I CTNAG 3 cut(s) 123, 1257, 1532
HpySE526I ACGT 3 cut(s) 677, 1007, 1023
Hsp92I GRCGYC 1 cut(s) 1400
HspAI GCGC 3 cut(s) 509, 596, 959
KflI GGGWCCC 1 cut(s) 852
Ksp22I TGATCA 1 cut(s) 85
KspI CCGCGG 1 cut(s) 714
Kzo9I GATC 5 cut(s) 85, 142, 1218, 1250, 1281
LmnI GCTCC 6 cut(s) 743, 751, 830, 838, 1243, 1423
LweI GCATC 8 cut(s) 406, 419, 877, 932, 970, 1315, 1455, 1549
MaeI CTAG 3 cut(s) 138, 309, 618
MaeII ACGT 3 cut(s) 677, 1007, 1023
MaeIII GTNAC 6 cut(s) 31, 43, 389, 761, 929, 1518
MalI GATC 5 cut(s) 87, 144, 1220, 1252, 1283
MbiI CCGCTC 2 cut(s) 746, 833
MboI GATC 5 cut(s) 85, 142, 1218, 1250, 1281
MhlI GDGCHC 4 cut(s) 171, 408, 756, 843
MlsI TGGCCA 3 cut(s) 204, 372, 1350
MluNI TGGCCA 3 cut(s) 204, 372, 1350
MlyI GAGTC 2 cut(s) 269, 1045
MmeI TCCRAC 1 cut(s) 567
Mox20I TGGCCA 3 cut(s) 204, 372, 1350
MscI TGGCCA 3 cut(s) 204, 372, 1350
MseI TTAA 3 cut(s) 729, 822, 1458
MslI CAYNNNNRTG 2 cut(s) 1259, 1323
Msp20I TGGCCA 3 cut(s) 204, 372, 1350
MspA1I CMGCKG 3 cut(s) 50, 713, 812
MspI CCGG 1 cut(s) 233
Mva1269I GAATGC 1 cut(s) 908
MvnI CGCG 2 cut(s) 713, 1517
NcoI CCATGG 3 cut(s) 327, 379, 1098
NdeII GATC 5 cut(s) 85, 142, 1218, 1250, 1281
NlaIV GGNNCC 3 cut(s) 853, 854, 1245
NmuCI GTSAC 5 cut(s) 31, 389, 761, 929, 1518
NspI RCATGY 4 cut(s) 137, 1000, 1322, 1497
OliI CACNNNNGTG 1 cut(s) 1259
PaeI GCATGC 1 cut(s) 137
PaeR7I CTCGAG 1 cut(s) 1414
PciI ACATGT 2 cut(s) 1318, 1493
PcsI WCGNNNNNNNCGW 3 cut(s) 189, 296, 1013
PctI GAATGC 1 cut(s) 908
PfeI GAWTC 1 cut(s) 1332
PflMI CCANNNNNTGG 1 cut(s) 1027
PleI GAGTC 2 cut(s) 268, 1044
PmaCI CACGTG 1 cut(s) 1024
PmlI CACGTG 1 cut(s) 1024
PpsI GAGTC 2 cut(s) 268, 1044
Ppu21I YACGTR 1 cut(s) 1024
PpuMI RGGWCCY 2 cut(s) 119, 852
PscI ACATGT 2 cut(s) 1318, 1493
Psp124BI GAGCTC 3 cut(s) 408, 756, 843
Psp5II RGGWCCY 2 cut(s) 119, 852
PspCI CACGTG 1 cut(s) 1024
PspEI GGTNACC 1 cut(s) 43
PspFI CCCAGC 3 cut(s) 50, 93, 1557
PspN4I GGNNCC 3 cut(s) 853, 854, 1245
PspPI GGNCC 3 cut(s) 119, 852, 915
PspPPI RGGWCCY 2 cut(s) 119, 852
PvuII CAGCTG 1 cut(s) 812
RsaI GTAC 6 cut(s) 191, 563, 902, 1065, 1238, 1317
RsaNI GTAC 6 cut(s) 190, 562, 901, 1064, 1237, 1316
RseI CAYNNNNRTG 2 cut(s) 1259, 1323
SacI GAGCTC 3 cut(s) 408, 756, 843
SacII CCGCGG 1 cut(s) 714
SaqAI TTAA 3 cut(s) 729, 822, 1458
Sau3AI GATC 5 cut(s) 85, 142, 1218, 1250, 1281
Sau96I GGNCC 3 cut(s) 119, 852, 915
SchI GAGTC 2 cut(s) 269, 1045
SduI GDGCHC 4 cut(s) 171, 408, 756, 843
SfaNI GCATC 8 cut(s) 406, 419, 877, 932, 970, 1315, 1455, 1549
Sfr274I CTCGAG 1 cut(s) 1414
Sfr303I CCGCGG 1 cut(s) 714
SgrBI CCGCGG 1 cut(s) 714
SinI GGWCC 3 cut(s) 119, 852, 915
SlaI CTCGAG 1 cut(s) 1414
SmiMI CAYNNNNRTG 2 cut(s) 1259, 1323
SmlI CTYRAG 1 cut(s) 1414
SmoI CTYRAG 1 cut(s) 1414
SphI GCATGC 1 cut(s) 137
SspMI CTAG 3 cut(s) 138, 309, 618
SstI GAGCTC 3 cut(s) 408, 756, 843
StyI CCWWGG 5 cut(s) 327, 373, 379, 660, 1098
TaaI ACNGT 3 cut(s) 395, 583, 905
TaiI ACGT 3 cut(s) 680, 1010, 1026
TatI WGTACW 2 cut(s) 1236, 1315
TauI GCSGC 6 cut(s) 350, 509, 530, 1045, 1048, 1175
TfiI GAWTC 1 cut(s) 1332
Tru1I TTAA 3 cut(s) 729, 822, 1458
Tru9I TTAA 3 cut(s) 729, 822, 1458
TscAI CASTG 2 cut(s) 45, 1381
TseFI GTSAC 5 cut(s) 31, 389, 761, 929, 1518
Tsp45I GTSAC 5 cut(s) 31, 389, 761, 929, 1518
TspDTI ATGAA 4 cut(s) 20, 297, 1292, 1563
TspGWI ACGGA 4 cut(s) 54, 438, 943, 1002
TspRI CASTG 2 cut(s) 45, 1381
Van91I CCANNNNNTGG 1 cut(s) 1027
VpaK11BI GGWCC 3 cut(s) 119, 852, 915
XceI RCATGY 4 cut(s) 137, 1000, 1322, 1497
XhoI CTCGAG 1 cut(s) 1414
XspI CTAG 3 cut(s) 138, 309, 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.