Rroxscaffold_3G00231680

Domain of unknown function (DUF4149)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0010354
Physical Location & Seq
Forward (+)
17028707 .. 17032213
3507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00231680.1

Sequence Viewer

Length: 1587 bp
ATGCACAAGATTACACCCGGACTTCAAGATTCTTCACCGACCGCCGATGTTCGGATTACACCCGGACTTCAATCGCCGCGGGAATTTGGTCTCCGAGCCCGGATCAACGAACCAAAAGAGGCAAGGAGAGGAAGTCATAGTGAAAAACGGGCGCGAGTGGTCGTTGTTGAGTACGAGGATCAACAAGGCCACAACCCCAACACCAAGGTCTCCATCTCGGCGGATCAAGATGGCCGCCGCAAATCCAACTCCGCTATTTCCAACAATGACATTTCAACGCCCTCTAAGGTGACGAGGGCCTTGGACAATGCTATGGAGAACATCAAAGAAGCCGCCTCGGTTCTTCCAAATCGAGGCCGGGGACAAGGCCAAGGCCTTTCGAAATCGAAAACCGATGAAGATGGTCACCACTATAGTAGCCCGAAAGAGCCGATATGCGACGCGTATGGGAAATGCAAGCACAAGATTGCCGTTGTTTTGGAGACCAAGAAAGATATTTTGAAGGAGACCGCACATGAAGCCAAAGAGACAAGACACAAGGTCATAGAGATCAAGACGGATATTGACAAGGGGACTGCACATGAAGTCAAGGAGACGGCTCGGAAGGTCGGAGAGGCGGTAGAAGATGCATACGACGCCGTTTACGAGAGAGCTCGAGGAGAAGATGCCTATGAGAGAGCCAAAGAGACGGTTTCGGATACAACACATGAGGTTGAAGAACGAGCCAAAGAGTCGGTGGACAAAGCAATGAACACGGCCAAGACAAGAAAGGAAATGGGAAAGACCTTAAGTGGAGACGTACAAAGAAACGTGTCCAAATTGAAAACAGCTCCGGAACATGTTAAAGAGAAAGCAAAAGAGGCCAAAGAGACAGTGGAGGATGCAGCAGAAAGAGCCAAAGAGACCATGGAGCACGCTACTTATAAGGTTAAGGCCGGGGCGAGAGGATATAAAGGATCGGTTGATCCTTTGTTATCCGGGCTGAGTTTGTTTGGGTTTGCCACTGCTTATGGCATGTCTATGTGGATAACATTTGCTTCAAGCTATGTGTTGTCTCGGGTGCTTTCCCCGCACCAGTTTGGGATGGTGCAGAGCAAGATTTACAAGGTTTATTTCGCGGCGATGGCCAGTACTGTTGGGATGGCATTGTTGGGCCATCTATTGAACCATTGGAAGAAGTTGACAGGGAGGAATGTTGACGCCTTACAGAACTACAATCTTTTGCTTGCCGAGTTGATGGTTCTGTTCAATATGCTATACTTGGAGCCAAGATCCACTAAGGTGATGTTTGAGAAAATGAAGTTGGAGAAAGAAGAAGGAAGAGGAAGAGAGCATGTTGGTGCCGAAACCAGCACCGAGGCTGAGCGGCATTCACTAACTGCCGAACCATCAACAACAACTCCCTCAGAAGTCCCAGAAGGGAGGGAGCGTAAGGTAGTTCAGCCTCAGGTTGACAGACTTAACGAGGCACATCAGAGGCTCAAGAAACTGAATTCCTACTCATCTATCCTCAACATCTTGTCACTAATGGCTCTTACTTGGCATCTAGTATACCTTGCTAAGCGACTGCAAAGTTCTTGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4149

Protein Analysis

528

Amino Acids

58.92

Weight (kDa)

9.06

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4149 PF13664 332 - 433 4.3e-22 Domain of unknown function (DUF4149)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22600 AT1G72100
fragaria_vesca FvH4_5g31970
malus_domestica MD15G1396400.v1.1 MD15G1407700.v1.1
prunus_persica Prupe.1G554000_v2.0.a1
pyrus_communis pycom15g36370
rosa_chinensis RchiOBHm_Chr7g0227991
rosa_laevigata RLG00000001601
rosa_multiflora Rmu_co8508245.1_g000001 Rmu_sc0006493.1_g000005
rosa_roxburghii Rroxscaffold_3G00231680
rosa_rugosa Rorug07G0244900
rosa_samantha Rh7AG398000 Rh7AG398300 Rh7AG398800 Rh7BG380100 Rh7CG416400 Rh7CG416900 Rh7DG394400
rosa_wichuraiana Rw7G033150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 924
AccB1I GGYRCC 1 cut(s) 1340
AccBSI CCGCTC 1 cut(s) 1366
AccI GTMKAC 1 cut(s) 1551
AccII CGCG 4 cut(s) 79, 154, 443, 1118
AccIII TCCGGA 1 cut(s) 832
AclWI GGATC 6 cut(s) 110, 186, 231, 959, 964, 1266
AcoI YGGCCR 3 cut(s) 232, 756, 1125
AcsI RAATTY 2 cut(s) 83, 1492
AcyI GRCGYC 2 cut(s) 636, 1200
AfaI GTAC 3 cut(s) 173, 801, 1132
AfiI CCNNNNNNNGG 2 cut(s) 51, 353
AflII CTTAAG 1 cut(s) 787
AflIII ACRYGT 3 cut(s) 441, 810, 838
AgsI TTSAA 9 cut(s) 26, 71, 276, 502, 716, 823, 1041, 1165, 1249
AhdI GACNNNNNGTC 1 cut(s) 539
AjuI GAANNNNNNNTTGG 2 cut(s) 1286, 1318
AleI CACNNNNGTG 1 cut(s) 1280
AluBI AGCT 3 cut(s) 653, 830, 1044
AluI AGCT 3 cut(s) 653, 830, 1044
Alw21I GWGCWC 2 cut(s) 655, 915
AlwI GGATC 6 cut(s) 110, 186, 231, 959, 964, 1266
Ama87I CYCGRG 2 cut(s) 654, 1056
Aor13HI TCCGGA 1 cut(s) 832
ApeKI GCWGC 2 cut(s) 884, 1581
ApoI RAATTY 2 cut(s) 83, 1492
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 2 cut(s) 297, 1153
AsuC2I CCSGG 6 cut(s) 18, 63, 100, 359, 937, 979
AsuHPI GGTGA 4 cut(s) 27, 301, 398, 1294
AsuII TTCGAA 1 cut(s) 380
AvaI CYCGRG 2 cut(s) 654, 1056
AxyI CCTNAGG 1 cut(s) 1446
BalI TGGCCA 1 cut(s) 1127
BanI GGYRCC 1 cut(s) 1340
BanII GRGCYC 2 cut(s) 100, 655
Bbv12I GWGCWC 2 cut(s) 655, 915
BbvI GCAGC 2 cut(s) 896, 1568
BccI CCATC 9 cut(s) 221, 224, 395, 1078, 1117, 1135, 1164, 1231, 1396
BceAI ACGGC 4 cut(s) 455, 612, 623, 771
BciVI GTATCC 1 cut(s) 691
BcnI CCSGG 6 cut(s) 18, 63, 100, 359, 937, 979
BfaI CTAG 1 cut(s) 1547
BfmI CTRYAG 1 cut(s) 412
BfrI CTTAAG 1 cut(s) 787
BfuI GTATCC 1 cut(s) 691
BisI GCNGC 8 cut(s) 77, 235, 238, 333, 885, 1119, 1367, 1582
BlpI GCTNAGC 2 cut(s) 1362, 1560
BlsI GCNGC 8 cut(s) 78, 236, 239, 334, 886, 1120, 1368, 1583
BmcAI AGTACT 1 cut(s) 1132
Bme1390I CCNGG 6 cut(s) 18, 63, 100, 359, 937, 979
BmeRI GACNNNNNGTC 1 cut(s) 539
BmeT110I CYCGRG 2 cut(s) 654, 1056
BmgT120I GGNCC 2 cut(s) 297, 1153
BmiI GGNNCC 2 cut(s) 1266, 1342
BmrFI CCNGG 6 cut(s) 18, 63, 100, 359, 937, 979
BmsI GCATC 4 cut(s) 616, 655, 871, 1552
Bpu1102I GCTNAGC 2 cut(s) 1362, 1560
Bpu14I TTCGAA 1 cut(s) 380
BpuEI CTTGAG 1 cut(s) 1466
BpuMI CCSGG 6 cut(s) 18, 63, 100, 359, 937, 979
BsaHI GRCGYC 2 cut(s) 636, 1200
BsaI GGTCTC 5 cut(s) 95, 214, 476, 500, 896
BsaJI CCNNGG 9 cut(s) 77, 204, 300, 336, 358, 370, 906, 936, 1356
BsaWI WCCGGW 1 cut(s) 832
BsaXI ACNNNNNCTCC 8 cut(s) 118, 148, 603, 633, 1384, 1414, 1414, 1444
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 353
Bse1I ACTGG 2 cut(s) 1075, 1128
Bse21I CCTNAGG 1 cut(s) 1446
Bse3DI GCAATG 1 cut(s) 753
BseAI TCCGGA 1 cut(s) 832
BseDI CCNNGG 9 cut(s) 77, 204, 300, 336, 358, 370, 906, 936, 1356
BseGI GGATG 3 cut(s) 886, 1089, 1146
BseLI CCNNNNNNNGG 2 cut(s) 51, 353
BseMI GCAATG 1 cut(s) 753
BseMII CTCAG 4 cut(s) 974, 1353, 1419, 1460
BseNI ACTGG 2 cut(s) 1075, 1128
BseRI GAGGAG 1 cut(s) 672
BseXI GCAGC 2 cut(s) 896, 1568
BsgI GTGCAG 2 cut(s) 561, 1109
Bsh1236I CGCG 4 cut(s) 79, 154, 443, 1118
Bsh1285I CGRYCG 1 cut(s) 42
BshNI GGYRCC 1 cut(s) 1340
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 2 cut(s) 655, 915
BsiHKCI CYCGRG 2 cut(s) 654, 1056
BsiSI CCGG 7 cut(s) 18, 63, 100, 358, 833, 936, 978
BslFI GGGAC 3 cut(s) 375, 586, 1397
BslI CCNNNNNNNGG 2 cut(s) 51, 353
BsmBI CGTCTC 3 cut(s) 587, 680, 789
BsmFI GGGAC 3 cut(s) 375, 586, 1397
BsmI GAATGC 1 cut(s) 1369
Bso31I GGTCTC 5 cut(s) 95, 214, 476, 500, 896
BsoBI CYCGRG 2 cut(s) 654, 1056
Bsp119I TTCGAA 1 cut(s) 380
Bsp1286I GDGCHC 3 cut(s) 100, 655, 915
Bsp13I TCCGGA 1 cut(s) 832
Bsp143I GATC 7 cut(s) 102, 178, 223, 549, 956, 964, 1271
Bsp1720I GCTNAGC 2 cut(s) 1362, 1560
Bsp19I CCATGG 1 cut(s) 906
BspCNI CTCAG 4 cut(s) 975, 1354, 1418, 1459
BspEI TCCGGA 1 cut(s) 832
BspFNI CGCG 4 cut(s) 79, 154, 443, 1118
BspLI GGNNCC 2 cut(s) 1266, 1342
BspPI GGATC 6 cut(s) 110, 186, 231, 959, 964, 1266
BspT104I TTCGAA 1 cut(s) 380
BspT107I GGYRCC 1 cut(s) 1340
BspTI CTTAAG 1 cut(s) 787
BspTNI GGTCTC 5 cut(s) 95, 214, 476, 500, 896
BsrBI CCGCTC 1 cut(s) 1366
BsrDI GCAATG 1 cut(s) 753
BsrI ACTGG 2 cut(s) 1075, 1128
BssECI CCNNGG 9 cut(s) 77, 204, 300, 336, 358, 370, 906, 936, 1356
BssMI GATC 7 cut(s) 102, 178, 223, 549, 956, 964, 1271
BssNAI GTATAC 1 cut(s) 1552
BssNI GRCGYC 2 cut(s) 636, 1200
BssT1I CCWWGG 4 cut(s) 204, 300, 370, 906
Bst1107I GTATAC 1 cut(s) 1552
Bst4CI ACNGT 3 cut(s) 691, 874, 1135
Bst6I CTCTTC 2 cut(s) 1315, 1321
BstACI GRCGYC 2 cut(s) 636, 1200
BstAFI CTTAAG 1 cut(s) 787
BstBI TTCGAA 1 cut(s) 380
BstC8I GCNNGC 3 cut(s) 458, 915, 1227
BstDEI CTNAG 7 cut(s) 285, 983, 1278, 1362, 1405, 1446, 1560
BstDSI CCRYGG 2 cut(s) 77, 906
BstEII GGTNACC 1 cut(s) 404
BstF5I GGATG 3 cut(s) 886, 1089, 1146
BstFNI CGCG 4 cut(s) 79, 154, 443, 1118
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 7 cut(s) 105, 181, 226, 552, 959, 967, 1274
BstMBI GATC 7 cut(s) 102, 178, 223, 549, 956, 964, 1271
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 6 cut(s) 518, 635, 860, 893, 1069, 1124
BstNSI RCATGY 3 cut(s) 842, 1018, 1337
BstPI GGTNACC 1 cut(s) 404
BstSCI CCNGG 6 cut(s) 16, 61, 98, 357, 935, 977
BstSFI CTRYAG 1 cut(s) 412
BstUI CGCG 4 cut(s) 79, 154, 443, 1118
BstV1I GCAGC 2 cut(s) 896, 1568
BstX2I RGATCY 1 cut(s) 1271
BstYI RGATCY 1 cut(s) 1271
BstZ17I GTATAC 1 cut(s) 1552
Bsu36I CCTNAGG 1 cut(s) 1446
BsuI GTATCC 1 cut(s) 691
BtgI CCRYGG 2 cut(s) 77, 906
BtgZI GCGATG 1 cut(s) 1136
BtsCI GGATG 3 cut(s) 886, 1089, 1146
BtsI GCAGTG 1 cut(s) 1002
BtsIMutI CAGTG 2 cut(s) 879, 1002
Cac8I GCNNGC 3 cut(s) 458, 915, 1227
CfoI GCGC 1 cut(s) 154
Cfr13I GGNCC 2 cut(s) 297, 1153
Cfr42I CCGCGG 1 cut(s) 80
CseI GACGC 3 cut(s) 449, 644, 1208
Csp6I GTAC 3 cut(s) 172, 800, 1131
CviAII CATG 7 cut(s) 515, 581, 707, 839, 907, 1015, 1334
CviQI GTAC 3 cut(s) 172, 800, 1131
DdeI CTNAG 7 cut(s) 285, 983, 1278, 1362, 1405, 1446, 1560
DpnI GATC 7 cut(s) 104, 180, 225, 551, 958, 966, 1273
DpnII GATC 7 cut(s) 102, 178, 223, 549, 956, 964, 1271
DriI GACNNNNNGTC 1 cut(s) 539
EaeI YGGCCR 3 cut(s) 232, 756, 1125
Eam1104I CTCTTC 2 cut(s) 1315, 1321
Eam1105I GACNNNNNGTC 1 cut(s) 539
EarI CTCTTC 2 cut(s) 1315, 1321
EciI GGCGGA 1 cut(s) 236
Ecl136II GAGCTC 1 cut(s) 653
Eco130I CCWWGG 4 cut(s) 204, 300, 370, 906
Eco147I AGGCCT 1 cut(s) 375
Eco24I GRGCYC 2 cut(s) 100, 655
Eco31I GGTCTC 5 cut(s) 95, 214, 476, 500, 896
Eco53kI GAGCTC 1 cut(s) 653
Eco81I CCTNAGG 1 cut(s) 1446
Eco88I CYCGRG 2 cut(s) 654, 1056
Eco91I GGTNACC 1 cut(s) 404
EcoICRI GAGCTC 1 cut(s) 653
EcoO109I RGGNCCY 1 cut(s) 297
EcoO65I GGTNACC 1 cut(s) 404
EcoRI GAATTC 1 cut(s) 1492
EcoT14I CCWWGG 4 cut(s) 204, 300, 370, 906
EcoT22I ATGCAT 1 cut(s) 631
EcoT38I GRGCYC 2 cut(s) 100, 655
ErhI CCWWGG 4 cut(s) 204, 300, 370, 906
Esp3I CGTCTC 3 cut(s) 587, 680, 789
FaeI CATG 7 cut(s) 518, 584, 710, 842, 910, 1018, 1337
FaqI GGGAC 3 cut(s) 375, 586, 1397
FatI CATG 7 cut(s) 514, 580, 706, 838, 906, 1014, 1333
FauI CCCGC 2 cut(s) 72, 1077
FblI GTMKAC 1 cut(s) 1551
Fnu4HI GCNGC 8 cut(s) 77, 235, 238, 333, 885, 1119, 1367, 1582
FokI GGATG 3 cut(s) 893, 1096, 1153
FriOI GRGCYC 2 cut(s) 100, 655
Fsp4HI GCNGC 8 cut(s) 77, 235, 238, 333, 885, 1119, 1367, 1582
FspBI CTAG 1 cut(s) 1547
GlaI GCGC 1 cut(s) 153
GluI GCNGC 8 cut(s) 77, 235, 238, 333, 885, 1119, 1367, 1582
HapII CCGG 7 cut(s) 18, 63, 100, 358, 833, 936, 978
HgaI GACGC 3 cut(s) 449, 644, 1208
HhaI GCGC 1 cut(s) 154
Hin1I GRCGYC 2 cut(s) 636, 1200
Hin1II CATG 7 cut(s) 518, 584, 710, 842, 910, 1018, 1337
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HincII GTYRAC 3 cut(s) 1182, 1198, 1453
HindII GTYRAC 3 cut(s) 1182, 1198, 1453
HinfI GANTC 2 cut(s) 29, 731
HpaII CCGG 7 cut(s) 18, 63, 100, 358, 833, 936, 978
HphI GGTGA 4 cut(s) 27, 301, 398, 1294
Hpy166II GTNNAC 6 cut(s) 643, 739, 1182, 1198, 1453, 1552
Hpy188I TCNGA 7 cut(s) 54, 95, 603, 611, 697, 1408, 1476
Hpy188III TCNNGA 5 cut(s) 26, 227, 553, 833, 1483
Hpy8I GTNNAC 6 cut(s) 643, 739, 1182, 1198, 1453, 1552
Hpy99I CGWCG 2 cut(s) 443, 638
HpyAV CCTTC 4 cut(s) 496, 598, 1310, 1412
HpyCH4III ACNGT 3 cut(s) 691, 874, 1135
HpyCH4IV ACGT 2 cut(s) 798, 810
HpyCH4V TGCA 7 cut(s) 4, 456, 578, 629, 884, 1090, 1570
HpyF10VI GCNNNNNNNGC 6 cut(s) 518, 635, 860, 893, 1069, 1124
HpyF3I CTNAG 7 cut(s) 285, 983, 1278, 1362, 1405, 1446, 1560
HpySE526I ACGT 2 cut(s) 798, 810
Hsp92I GRCGYC 2 cut(s) 636, 1200
Hsp92II CATG 7 cut(s) 518, 584, 710, 842, 910, 1018, 1337
HspAI GCGC 1 cut(s) 152
Kpn2I TCCGGA 1 cut(s) 832
KspI CCGCGG 1 cut(s) 80
Kzo9I GATC 7 cut(s) 102, 178, 223, 549, 956, 964, 1271
LmnI GCTCC 4 cut(s) 835, 910, 1264, 1426
Lsp1109I GCAGC 2 cut(s) 896, 1568
LweI GCATC 4 cut(s) 616, 655, 871, 1552
MaeI CTAG 1 cut(s) 1547
MaeII ACGT 2 cut(s) 798, 810
MaeIII GTNAC 3 cut(s) 289, 404, 1521
MalI GATC 7 cut(s) 104, 180, 225, 551, 958, 966, 1273
MbiI CCGCTC 1 cut(s) 1366
MboI GATC 7 cut(s) 102, 178, 223, 549, 956, 964, 1271
MflI RGATCY 1 cut(s) 1271
MhlI GDGCHC 3 cut(s) 100, 655, 915
MlsI TGGCCA 1 cut(s) 1127
MluCI AATT 3 cut(s) 83, 818, 1492
MluI ACGCGT 1 cut(s) 441
MluNI TGGCCA 1 cut(s) 1127
MlyI GAGTC 1 cut(s) 740
MmeI TCCRAC 4 cut(s) 270, 285, 589, 1284
Mox20I TGGCCA 1 cut(s) 1127
Mph1103I ATGCAT 1 cut(s) 631
MroI TCCGGA 1 cut(s) 832
MscI TGGCCA 1 cut(s) 1127
MseI TTAA 4 cut(s) 788, 843, 930, 1461
MslI CAYNNNNRTG 3 cut(s) 1019, 1280, 1338
Msp20I TGGCCA 1 cut(s) 1127
MspA1I CMGCKG 1 cut(s) 79
MspCI CTTAAG 1 cut(s) 787
MspI CCGG 7 cut(s) 18, 63, 100, 358, 833, 936, 978
MspR9I CCNGG 6 cut(s) 18, 63, 100, 359, 937, 979
Mva1269I GAATGC 1 cut(s) 1369
MvnI CGCG 4 cut(s) 79, 154, 443, 1118
MwoI GCNNNNNNNGC 6 cut(s) 518, 635, 860, 893, 1069, 1124
NciI CCSGG 6 cut(s) 18, 63, 100, 359, 937, 979
NcoI CCATGG 1 cut(s) 906
NdeII GATC 7 cut(s) 102, 178, 223, 549, 956, 964, 1271
NlaIII CATG 7 cut(s) 518, 584, 710, 842, 910, 1018, 1337
NlaIV GGNNCC 2 cut(s) 1266, 1342
NmeAIII GCCGAG 2 cut(s) 197, 1255
NmuCI GTSAC 3 cut(s) 289, 404, 1521
NsiI ATGCAT 1 cut(s) 631
NspI RCATGY 3 cut(s) 842, 1018, 1337
NspV TTCGAA 1 cut(s) 380
OliI CACNNNNGTG 1 cut(s) 1280
PaeR7I CTCGAG 1 cut(s) 654
PceI AGGCCT 1 cut(s) 375
PciI ACATGT 1 cut(s) 838
PcsI WCGNNNNNNNCGW 1 cut(s) 642
PctI GAATGC 1 cut(s) 1369
PfeI GAWTC 1 cut(s) 29
PkrI GCNGC 8 cut(s) 78, 236, 239, 334, 886, 1120, 1368, 1583
PleI GAGTC 1 cut(s) 739
PpsI GAGTC 1 cut(s) 739
PscI ACATGT 1 cut(s) 838
PsiI TTATAA 1 cut(s) 924
Psp124BI GAGCTC 1 cut(s) 655
PspEI GGTNACC 1 cut(s) 404
PspN4I GGNNCC 2 cut(s) 1266, 1342
PspPI GGNCC 2 cut(s) 297, 1153
PspXI VCTCGAGB 1 cut(s) 654
PsuI RGATCY 1 cut(s) 1271
RsaI GTAC 3 cut(s) 173, 801, 1132
RsaNI GTAC 3 cut(s) 172, 800, 1131
RseI CAYNNNNRTG 3 cut(s) 1019, 1280, 1338
SacI GAGCTC 1 cut(s) 655
SacII CCGCGG 1 cut(s) 80
SaqAI TTAA 4 cut(s) 788, 843, 930, 1461
SatI GCNGC 8 cut(s) 77, 235, 238, 333, 885, 1119, 1367, 1582
Sau3AI GATC 7 cut(s) 102, 178, 223, 549, 956, 964, 1271
Sau96I GGNCC 2 cut(s) 297, 1153
ScaI AGTACT 1 cut(s) 1132
SchI GAGTC 1 cut(s) 740
ScrFI CCNGG 6 cut(s) 18, 63, 100, 359, 937, 979
SduI GDGCHC 3 cut(s) 100, 655, 915
SfaNI GCATC 4 cut(s) 616, 655, 871, 1552
SfcI CTRYAG 1 cut(s) 412
Sfr274I CTCGAG 1 cut(s) 654
Sfr303I CCGCGG 1 cut(s) 80
SfuI TTCGAA 1 cut(s) 380
SgrBI CCGCGG 1 cut(s) 80
SlaI CTCGAG 1 cut(s) 654
SmiMI CAYNNNNRTG 3 cut(s) 1019, 1280, 1338
SmlI CTYRAG 3 cut(s) 654, 787, 1481
SmoI CTYRAG 3 cut(s) 654, 787, 1481
Sse9I AATT 3 cut(s) 83, 818, 1492
SseBI AGGCCT 1 cut(s) 375
SspMI CTAG 1 cut(s) 1547
SstI GAGCTC 1 cut(s) 655
StuI AGGCCT 1 cut(s) 375
StyD4I CCNGG 6 cut(s) 16, 61, 98, 357, 935, 977
StyI CCWWGG 4 cut(s) 204, 300, 370, 906
TaaI ACNGT 3 cut(s) 691, 874, 1135
TaiI ACGT 2 cut(s) 801, 813
TaqI TCGA 4 cut(s) 352, 380, 386, 655
TasI AATT 3 cut(s) 83, 818, 1492
TatI WGTACW 1 cut(s) 1130
TauI GCSGC 6 cut(s) 79, 237, 240, 335, 1121, 1369
TfiI GAWTC 1 cut(s) 29
Tru1I TTAA 4 cut(s) 788, 843, 930, 1461
Tru9I TTAA 4 cut(s) 788, 843, 930, 1461
TscAI CASTG 2 cut(s) 879, 1009
TseFI GTSAC 3 cut(s) 289, 404, 1521
TseI GCWGC 2 cut(s) 884, 1581
Tsp45I GTSAC 3 cut(s) 289, 404, 1521
TspDTI ATGAA 5 cut(s) 411, 531, 597, 764, 1313
TspGWI ACGGA 1 cut(s) 572
TspRI CASTG 2 cut(s) 879, 1009
Vha464I CTTAAG 1 cut(s) 787
XapI RAATTY 2 cut(s) 83, 1492
XceI RCATGY 3 cut(s) 842, 1018, 1337
XcmI CCANNNNNNNNNTGG 3 cut(s) 733, 871, 904
XhoI CTCGAG 1 cut(s) 654
XmiI GTMKAC 1 cut(s) 1551
XspI CTAG 1 cut(s) 1547
ZrmI AGTACT 1 cut(s) 1132
Zsp2I ATGCAT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.