RLG00000001601

Domain of unknown function (DUF4149)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0010354
Physical Location & Seq
Forward (+)
18013167 .. 18015695
2529 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001601

Sequence Viewer

Length: 1764 bp
ATGATGAATGTTGTCGCTTTGTTCCTCGTTGTGACTTCACTAGTCAGCGCCGGAATTTGGTCTCCGAGCCCAGATCAACAGAACCAAAAGAGGCAAGGAGAGGAAGTCATAGTGAAAAACGGGCACAGAGTGGTGGTTGTTGAGTACGAGGATCAACAAGGCCACAACCCCAACACCAAGGTCTTCATCTCGCCGGATCAAGATGGCCGCCGCAAATCCAGCTCCGCTATTTCCAACAATGACATTTCGACGCCCTCCAAGGTGACGAGGGCCTTGGACAATGCTATGGAGAACATCAAAGAAGCCGCCGCGTCGGTTCTTCCAAATCGAGGCCAGGGACAAGGCCAAGGCCTTTCGAAATCGAAAACTGATGAAGATGGCCACCACTATAGTAGCCCGAAAGAGCTGATATGCGACGCGTATGGGAAATGCAAGCACAAGATTGCCAGTGCTTTGGAGACCAAGAAAGATATTTTGAAGGAGACTGCACATGAAGCCAAAGAGACAGCACACAAGGTCATAGAGATCAAGAAGGATATTGACAAGCGGACTGCACATGAAGTCAAGGAGACGGCTCAGAAGGTCGGAGAGGCGGTGGAAGATGCATACGACGCCGTTTCCGAAAGAGCTCAGGAGGTTGGAGAGCGTGCCAAGGAGGCTGCACACAGGGCCAAAGATACAGTAGGAGGGGCATATGGAAGGGCCAAGGATACAGTAGGAGGGGCATATGGAAGAGCCAAGGATACAGTAGAAGATGCCTATGAGAGAGCCAAAGAGACGGTTTCGGATACAACACATGAGGTTGAAGAACGAGACAAAGAGTCGGTGGACAAAGCAATGAACACGGCCAAGACAGCAAAGGAAATGGGAAAGACCTTAAGTGGAGACGTACAAAGAAATGTGTCCAAATTGAAAACAGCTCCGGAACATGTTAAAGAGAAAGCAAAAGAGGCCAAAGACACAGTGGAGGATGCAGCAGAAGGAGCCAAAGAGACCATGGAGCACGCTGCTTGTAAGGTTAAGGCCGGGGCAGAGGATATAAAGGATGCGACCGATAGGAGGTTGAATCGAGTGTTTAAGAAAGGAAGAGATATCTTGTATGGTGTGTTAAGGTCTATGGGGTCATTGTTTCCAGCGGTTGATCCTTTGTTATCCGGGCTGAGTTTGTTTGGGTTTGCCACTGCTTATGGCATGTCTATGTGGATAACATTTGCTTCAAGCTATGTGTTGTCTCGGGTGCTTTCCCCGCACCAGTTTGGGATGGTGCAGAGCAAGATTTACAAGGTTTATTTCGCAGCGATGGCCAGTACTGTTGGGATGGCATTGTTGGGCCATCTATTGAACCATTGGAAGAAGTTGACAGGGAGGAATGTTGACGCCTTACAGAACTACAATCTTTTGCTTGCCGAGTTAATGGTTCTGTTCAATATGTTACACTTGGAGCCAAGATCCACTAAGATGATGTTTGAGAAACTGAAGTTGGAGAAAGAAGAAGGAAGAGGAAGAGAGCATGTTCGTGCCAAAACCAGCACGGAGGCTGAGCGGCAGCCACTTACTGCCGAACCCTCAACTACAACTCCCTCAGAAGTCCCAGAAGGGAGGGAGCGTGAGGTAGTTCAGCCTCAGGTTGACAGACTTAAGGAGGCACATCAGACGCTCAAGAAGCTGAATTCCTACTCGTCTATCCTCAACATCTTGTCACTAATGGCTCTTACTTGGCATCTAGTATACCTTGCTAAGCGACTGCAAAGTTCTTGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4149

Protein Analysis

588

Amino Acids

64.95

Weight (kDa)

8.59

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4149 PF13664 391 - 491 5.7e-21 Domain of unknown function (DUF4149)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22600 AT1G72100
fragaria_vesca FvH4_5g31970
malus_domestica MD15G1396400.v1.1 MD15G1407700.v1.1
prunus_persica Prupe.1G554000_v2.0.a1
pyrus_communis pycom15g36370
rosa_chinensis RchiOBHm_Chr7g0227991
rosa_laevigata RLG00000001601
rosa_multiflora Rmu_co8508245.1_g000001 Rmu_sc0006493.1_g000005
rosa_roxburghii Rroxscaffold_3G00231680
rosa_rugosa Rorug07G0244900
rosa_samantha Rh7AG398000 Rh7AG398300 Rh7AG398800 Rh7BG380100 Rh7CG416400 Rh7CG416900 Rh7DG394400
rosa_wichuraiana Rw7G033150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1543
AccI GTMKAC 1 cut(s) 1728
AccII CGCG 2 cut(s) 311, 419
AccIII TCCGGA 1 cut(s) 922
AclWI GGATC 4 cut(s) 159, 204, 1136, 1443
AcoI YGGCCR 4 cut(s) 205, 379, 846, 1302
AcsI RAATTY 2 cut(s) 54, 1669
AcuI CTGAAG 1 cut(s) 1496
AcyI GRCGYC 3 cut(s) 251, 612, 1377
AdeI CACNNNGTG 1 cut(s) 130
AfaI GTAC 3 cut(s) 146, 891, 1309
AfiI CCNNNNNNNGG 3 cut(s) 57, 329, 1059
AflII CTTAAG 2 cut(s) 877, 1637
AflIII ACRYGT 2 cut(s) 417, 928
AgsI TTSAA 7 cut(s) 478, 806, 913, 1066, 1218, 1342, 1426
AhdI GACNNNNNGTC 1 cut(s) 820
AhlI ACTAGT 1 cut(s) 40
AjnI CCWGG 1 cut(s) 333
AjuI GAANNNNNNNTTGG 2 cut(s) 1463, 1495
AluBI AGCT 6 cut(s) 222, 406, 629, 920, 1221, 1666
AluI AGCT 6 cut(s) 222, 406, 629, 920, 1221, 1666
Alw21I GWGCWC 2 cut(s) 631, 1005
AlwI GGATC 4 cut(s) 159, 204, 1136, 1443
Ama87I CYCGRG 1 cut(s) 1233
Aor13HI TCCGGA 1 cut(s) 922
ApeKI GCWGC 6 cut(s) 659, 974, 1007, 1295, 1546, 1758
ApoI RAATTY 2 cut(s) 54, 1669
AspLEI GCGC 1 cut(s) 50
AspS9I GGNCC 4 cut(s) 270, 669, 702, 1330
AsuC2I CCSGG 2 cut(s) 1027, 1156
AsuHPI GGTGA 1 cut(s) 274
AsuII TTCGAA 1 cut(s) 356
AvaI CYCGRG 1 cut(s) 1233
AxyI CCTNAGG 1 cut(s) 1623
BaeGI GKGCMC 1 cut(s) 126
BalI TGGCCA 2 cut(s) 381, 1304
BanII GRGCYC 2 cut(s) 71, 631
BbsI GAAGAC 1 cut(s) 175
Bbv12I GWGCWC 2 cut(s) 631, 1005
BbvI GCAGC 6 cut(s) 646, 986, 994, 1307, 1558, 1745
BccI CCATC 6 cut(s) 197, 371, 1255, 1294, 1312, 1341
BceAI ACGGC 3 cut(s) 588, 599, 861
BciT130I CCWGG 1 cut(s) 335
BciVI GTATCC 3 cut(s) 703, 736, 781
BcnI CCSGG 2 cut(s) 1027, 1156
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 2 cut(s) 41, 1724
BfmI CTRYAG 1 cut(s) 388
BfoI RGCGCY 1 cut(s) 51
BfrI CTTAAG 2 cut(s) 877, 1637
BfuI GTATCC 3 cut(s) 703, 736, 781
BglI GCCNNNNNGGC 1 cut(s) 656
BlpI GCTNAGC 2 cut(s) 1539, 1737
BmcAI AGTACT 1 cut(s) 1309
Bme1390I CCNGG 3 cut(s) 335, 1027, 1156
BmeRI GACNNNNNGTC 1 cut(s) 820
BmeT110I CYCGRG 1 cut(s) 1233
BmgT120I GGNCC 4 cut(s) 270, 669, 702, 1330
BmiI GGNNCC 2 cut(s) 985, 1443
BmrFI CCNGG 3 cut(s) 335, 1027, 1156
BmsI GCATC 5 cut(s) 592, 745, 961, 1036, 1729
BpiI GAAGAC 1 cut(s) 175
Bpu10I CCTNAGC 1 cut(s) 630
Bpu1102I GCTNAGC 2 cut(s) 1539, 1737
Bpu14I TTCGAA 1 cut(s) 356
BpuEI CTTGAG 1 cut(s) 1643
BpuMI CCSGG 2 cut(s) 1027, 1156
BsaHI GRCGYC 3 cut(s) 251, 612, 1377
BsaI GGTCTC 3 cut(s) 66, 452, 986
BsaWI WCCGGW 1 cut(s) 922
BsaXI ACNNNNNCTCC 8 cut(s) 90, 120, 579, 609, 1561, 1591, 1591, 1621
Bsc4I CCNNNNNNNGG 3 cut(s) 57, 329, 1059
Bse1I ACTGG 3 cut(s) 447, 1252, 1305
Bse21I CCTNAGG 1 cut(s) 1623
Bse3DI GCAATG 1 cut(s) 843
BseAI TCCGGA 1 cut(s) 922
BseBI CCWGG 1 cut(s) 335
BseGI GGATG 4 cut(s) 976, 1051, 1266, 1323
BseLI CCNNNNNNNGG 3 cut(s) 57, 329, 1059
BseMI GCAATG 1 cut(s) 843
BseMII CTCAG 6 cut(s) 590, 644, 1151, 1530, 1596, 1637
BseNI ACTGG 3 cut(s) 447, 1252, 1305
BseSI GKGCMC 1 cut(s) 126
BseXI GCAGC 6 cut(s) 646, 986, 994, 1307, 1558, 1745
BsgI GTGCAG 4 cut(s) 471, 537, 645, 1286
Bsh1236I CGCG 2 cut(s) 311, 419
Bsh1285I CGRYCG 1 cut(s) 1053
BsiEI CGRYCG 1 cut(s) 1053
BsiHKAI GWGCWC 2 cut(s) 631, 1005
BsiHKCI CYCGRG 1 cut(s) 1233
BsiSI CCGG 5 cut(s) 51, 194, 923, 1026, 1155
BslFI GGGAC 2 cut(s) 351, 1574
BslI CCNNNNNNNGG 3 cut(s) 57, 329, 1059
BsmBI CGTCTC 3 cut(s) 563, 770, 879
BsmFI GGGAC 2 cut(s) 351, 1574
Bso31I GGTCTC 3 cut(s) 66, 452, 986
BsoBI CYCGRG 1 cut(s) 1233
Bsp119I TTCGAA 1 cut(s) 356
Bsp1286I GDGCHC 4 cut(s) 71, 126, 631, 1005
Bsp13I TCCGGA 1 cut(s) 922
Bsp143I GATC 6 cut(s) 73, 151, 196, 525, 1141, 1448
Bsp1720I GCTNAGC 2 cut(s) 1539, 1737
Bsp19I CCATGG 1 cut(s) 996
BspCNI CTCAG 6 cut(s) 589, 643, 1152, 1531, 1595, 1636
BspEI TCCGGA 1 cut(s) 922
BspFNI CGCG 2 cut(s) 311, 419
BspLI GGNNCC 2 cut(s) 985, 1443
BspPI GGATC 4 cut(s) 159, 204, 1136, 1443
BspQI GCTCTTC 1 cut(s) 727
BspT104I TTCGAA 1 cut(s) 356
BspTI CTTAAG 2 cut(s) 877, 1637
BspTNI GGTCTC 3 cut(s) 66, 452, 986
BsrBI CCGCTC 1 cut(s) 1543
BsrDI GCAATG 1 cut(s) 843
BsrI ACTGG 3 cut(s) 447, 1252, 1305
BssMI GATC 6 cut(s) 73, 151, 196, 525, 1141, 1448
BssNAI GTATAC 1 cut(s) 1729
BssNI GRCGYC 3 cut(s) 251, 612, 1377
BssT1I CCWWGG 8 cut(s) 177, 258, 273, 346, 651, 705, 738, 996
Bst1107I GTATAC 1 cut(s) 1729
Bst2UI CCWGG 1 cut(s) 335
Bst4CI ACNGT 6 cut(s) 682, 715, 748, 781, 964, 1312
Bst6I CTCTTC 4 cut(s) 727, 1081, 1492, 1498
BstACI GRCGYC 3 cut(s) 251, 612, 1377
BstAFI CTTAAG 2 cut(s) 877, 1637
BstBI TTCGAA 1 cut(s) 356
BstC8I GCNNGC 4 cut(s) 434, 648, 1005, 1404
BstDEI CTNAG 8 cut(s) 576, 630, 1160, 1455, 1539, 1582, 1623, 1737
BstDSI CCRYGG 1 cut(s) 996
BstF5I GGATG 4 cut(s) 976, 1051, 1266, 1323
BstFNI CGCG 2 cut(s) 311, 419
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 6 cut(s) 76, 154, 199, 528, 1144, 1451
BstMBI GATC 6 cut(s) 73, 151, 196, 525, 1141, 1448
BstMCI CGRYCG 1 cut(s) 1053
BstNI CCWGG 1 cut(s) 335
BstNSI RCATGY 3 cut(s) 932, 1195, 1514
BstSCI CCNGG 3 cut(s) 333, 1025, 1154
BstSFI CTRYAG 1 cut(s) 388
BstSLI GKGCMC 1 cut(s) 126
BstUI CGCG 2 cut(s) 311, 419
BstV1I GCAGC 6 cut(s) 646, 986, 994, 1307, 1558, 1745
BstV2I GAAGAC 1 cut(s) 175
BstX2I RGATCY 1 cut(s) 1448
BstXI CCANNNNNNTGG 1 cut(s) 454
BstYI RGATCY 1 cut(s) 1448
BstZ17I GTATAC 1 cut(s) 1729
Bsu36I CCTNAGG 1 cut(s) 1623
BsuI GTATCC 3 cut(s) 703, 736, 781
BtgI CCRYGG 1 cut(s) 996
BtgZI GCGATG 1 cut(s) 1313
BtsCI GGATG 4 cut(s) 976, 1051, 1266, 1323
BtsI GCAGTG 1 cut(s) 1179
BtsIMutI CAGTG 3 cut(s) 454, 969, 1179
Cac8I GCNNGC 4 cut(s) 434, 648, 1005, 1404
CfoI GCGC 1 cut(s) 50
Cfr13I GGNCC 4 cut(s) 270, 669, 702, 1330
CseI GACGC 6 cut(s) 259, 300, 425, 620, 1385, 1663
Csp6I GTAC 3 cut(s) 145, 890, 1308
CviAII CATG 7 cut(s) 491, 557, 797, 929, 997, 1192, 1511
CviQI GTAC 3 cut(s) 145, 890, 1308
DdeI CTNAG 8 cut(s) 576, 630, 1160, 1455, 1539, 1582, 1623, 1737
DpnI GATC 6 cut(s) 75, 153, 198, 527, 1143, 1450
DpnII GATC 6 cut(s) 73, 151, 196, 525, 1141, 1448
DraIII CACNNNGTG 1 cut(s) 130
DriI GACNNNNNGTC 1 cut(s) 820
EaeI YGGCCR 4 cut(s) 205, 379, 846, 1302
Eam1104I CTCTTC 4 cut(s) 727, 1081, 1492, 1498
Eam1105I GACNNNNNGTC 1 cut(s) 820
EarI CTCTTC 4 cut(s) 727, 1081, 1492, 1498
Ecl136II GAGCTC 1 cut(s) 629
Eco130I CCWWGG 8 cut(s) 177, 258, 273, 346, 651, 705, 738, 996
Eco147I AGGCCT 1 cut(s) 351
Eco24I GRGCYC 2 cut(s) 71, 631
Eco31I GGTCTC 3 cut(s) 66, 452, 986
Eco32I GATATC 1 cut(s) 1093
Eco53kI GAGCTC 1 cut(s) 629
Eco57I CTGAAG 1 cut(s) 1496
Eco81I CCTNAGG 1 cut(s) 1623
Eco88I CYCGRG 1 cut(s) 1233
EcoICRI GAGCTC 1 cut(s) 629
EcoO109I RGGNCCY 1 cut(s) 270
EcoRI GAATTC 1 cut(s) 1669
EcoRII CCWGG 1 cut(s) 333
EcoRV GATATC 1 cut(s) 1093
EcoT14I CCWWGG 8 cut(s) 177, 258, 273, 346, 651, 705, 738, 996
EcoT22I ATGCAT 1 cut(s) 607
EcoT38I GRGCYC 2 cut(s) 71, 631
ErhI CCWWGG 8 cut(s) 177, 258, 273, 346, 651, 705, 738, 996
Esp3I CGTCTC 3 cut(s) 563, 770, 879
FaeI CATG 7 cut(s) 494, 560, 800, 932, 1000, 1195, 1514
FaqI GGGAC 2 cut(s) 351, 1574
FatI CATG 7 cut(s) 490, 556, 796, 928, 996, 1191, 1510
FauI CCCGC 1 cut(s) 1254
FauNDI CATATG 2 cut(s) 694, 727
FblI GTMKAC 1 cut(s) 1728
FokI GGATG 4 cut(s) 983, 1058, 1273, 1330
FriOI GRGCYC 2 cut(s) 71, 631
FspBI CTAG 2 cut(s) 41, 1724
GlaI GCGC 1 cut(s) 49
HaeII RGCGCY 1 cut(s) 51
HapII CCGG 5 cut(s) 51, 194, 923, 1026, 1155
HgaI GACGC 6 cut(s) 259, 300, 425, 620, 1385, 1663
HhaI GCGC 1 cut(s) 50
Hin1I GRCGYC 3 cut(s) 251, 612, 1377
Hin1II CATG 7 cut(s) 494, 560, 800, 932, 1000, 1195, 1514
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HincII GTYRAC 3 cut(s) 1359, 1375, 1630
HindII GTYRAC 3 cut(s) 1359, 1375, 1630
HinfI GANTC 2 cut(s) 821, 1066
HpaII CCGG 5 cut(s) 51, 194, 923, 1026, 1155
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 5 cut(s) 829, 1359, 1375, 1630, 1729
Hpy188I TCNGA 7 cut(s) 66, 579, 587, 622, 787, 1585, 1653
Hpy188III TCNNGA 5 cut(s) 200, 529, 632, 923, 1660
Hpy8I GTNNAC 5 cut(s) 829, 1359, 1375, 1630, 1729
Hpy99I CGWCG 4 cut(s) 253, 316, 419, 614
HpyAV CCTTC 7 cut(s) 472, 526, 574, 693, 974, 1487, 1589
HpyCH4III ACNGT 6 cut(s) 682, 715, 748, 781, 964, 1312
HpyCH4IV ACGT 1 cut(s) 888
HpyCH4V TGCA 8 cut(s) 432, 488, 554, 605, 662, 974, 1267, 1747
HpyF3I CTNAG 8 cut(s) 576, 630, 1160, 1455, 1539, 1582, 1623, 1737
HpySE526I ACGT 1 cut(s) 888
Hsp92I GRCGYC 3 cut(s) 251, 612, 1377
Hsp92II CATG 7 cut(s) 494, 560, 800, 932, 1000, 1195, 1514
HspAI GCGC 1 cut(s) 48
Kpn2I TCCGGA 1 cut(s) 922
Kzo9I GATC 6 cut(s) 73, 151, 196, 525, 1141, 1448
LguI GCTCTTC 1 cut(s) 727
LmnI GCTCC 6 cut(s) 227, 925, 983, 1000, 1441, 1603
Lsp1109I GCAGC 6 cut(s) 646, 986, 994, 1307, 1558, 1745
LweI GCATC 5 cut(s) 592, 745, 961, 1036, 1729
MaeI CTAG 2 cut(s) 41, 1724
MaeII ACGT 1 cut(s) 888
MaeIII GTNAC 4 cut(s) 31, 262, 1431, 1698
MalI GATC 6 cut(s) 75, 153, 198, 527, 1143, 1450
MbiI CCGCTC 1 cut(s) 1543
MboI GATC 6 cut(s) 73, 151, 196, 525, 1141, 1448
MflI RGATCY 1 cut(s) 1448
MhlI GDGCHC 4 cut(s) 71, 126, 631, 1005
MlsI TGGCCA 2 cut(s) 381, 1304
MluCI AATT 3 cut(s) 54, 908, 1669
MluI ACGCGT 1 cut(s) 417
MluNI TGGCCA 2 cut(s) 381, 1304
MlyI GAGTC 1 cut(s) 830
MmeI TCCRAC 4 cut(s) 258, 565, 619, 1461
Mox20I TGGCCA 2 cut(s) 381, 1304
Mph1103I ATGCAT 1 cut(s) 607
MroI TCCGGA 1 cut(s) 922
MscI TGGCCA 2 cut(s) 381, 1304
MseI TTAA 7 cut(s) 878, 933, 1020, 1077, 1109, 1412, 1638
MslI CAYNNNNRTG 3 cut(s) 1196, 1457, 1515
Msp20I TGGCCA 2 cut(s) 381, 1304
MspA1I CMGCKG 1 cut(s) 1136
MspCI CTTAAG 2 cut(s) 877, 1637
MspI CCGG 5 cut(s) 51, 194, 923, 1026, 1155
MspR9I CCNGG 3 cut(s) 335, 1027, 1156
MvaI CCWGG 1 cut(s) 335
MvnI CGCG 2 cut(s) 311, 419
NciI CCSGG 2 cut(s) 1027, 1156
NcoI CCATGG 1 cut(s) 996
NdeI CATATG 2 cut(s) 694, 727
NdeII GATC 6 cut(s) 73, 151, 196, 525, 1141, 1448
NlaIII CATG 7 cut(s) 494, 560, 800, 932, 1000, 1195, 1514
NlaIV GGNNCC 2 cut(s) 985, 1443
NmeAIII GCCGAG 1 cut(s) 1432
NmuCI GTSAC 3 cut(s) 31, 262, 1698
NsiI ATGCAT 1 cut(s) 607
NspI RCATGY 3 cut(s) 932, 1195, 1514
NspV TTCGAA 1 cut(s) 356
PceI AGGCCT 1 cut(s) 351
PciI ACATGT 1 cut(s) 928
PciSI GCTCTTC 1 cut(s) 727
PcsI WCGNNNNNNNCGW 1 cut(s) 618
PfeI GAWTC 1 cut(s) 1066
PleI GAGTC 1 cut(s) 829
PpsI GAGTC 1 cut(s) 829
PscI ACATGT 1 cut(s) 928
Psp124BI GAGCTC 1 cut(s) 631
Psp6I CCWGG 1 cut(s) 333
PspGI CCWGG 1 cut(s) 333
PspN4I GGNNCC 2 cut(s) 985, 1443
PspPI GGNCC 4 cut(s) 270, 669, 702, 1330
PsuI RGATCY 1 cut(s) 1448
RsaI GTAC 3 cut(s) 146, 891, 1309
RsaNI GTAC 3 cut(s) 145, 890, 1308
RseI CAYNNNNRTG 3 cut(s) 1196, 1457, 1515
SacI GAGCTC 1 cut(s) 631
SapI GCTCTTC 1 cut(s) 727
SaqAI TTAA 7 cut(s) 878, 933, 1020, 1077, 1109, 1412, 1638
Sau3AI GATC 6 cut(s) 73, 151, 196, 525, 1141, 1448
Sau96I GGNCC 4 cut(s) 270, 669, 702, 1330
ScaI AGTACT 1 cut(s) 1309
SchI GAGTC 1 cut(s) 830
ScrFI CCNGG 3 cut(s) 335, 1027, 1156
SduI GDGCHC 4 cut(s) 71, 126, 631, 1005
SfaNI GCATC 5 cut(s) 592, 745, 961, 1036, 1729
SfcI CTRYAG 1 cut(s) 388
SfuI TTCGAA 1 cut(s) 356
SmiMI CAYNNNNRTG 3 cut(s) 1196, 1457, 1515
SmlI CTYRAG 3 cut(s) 877, 1637, 1658
SmoI CTYRAG 3 cut(s) 877, 1637, 1658
SpeI ACTAGT 1 cut(s) 40
Sse9I AATT 3 cut(s) 54, 908, 1669
SseBI AGGCCT 1 cut(s) 351
SspMI CTAG 2 cut(s) 41, 1724
SstI GAGCTC 1 cut(s) 631
StuI AGGCCT 1 cut(s) 351
StyD4I CCNGG 3 cut(s) 333, 1025, 1154
StyI CCWWGG 8 cut(s) 177, 258, 273, 346, 651, 705, 738, 996
TaaI ACNGT 6 cut(s) 682, 715, 748, 781, 964, 1312
TaiI ACGT 1 cut(s) 891
TaqI TCGA 5 cut(s) 248, 328, 356, 362, 1069
TaqII GACCGA 1 cut(s) 1067
TasI AATT 3 cut(s) 54, 908, 1669
TatI WGTACW 1 cut(s) 1307
TauI GCSGC 5 cut(s) 210, 213, 308, 311, 1546
TfiI GAWTC 1 cut(s) 1066
Tru1I TTAA 7 cut(s) 878, 933, 1020, 1077, 1109, 1412, 1638
Tru9I TTAA 7 cut(s) 878, 933, 1020, 1077, 1109, 1412, 1638
TscAI CASTG 3 cut(s) 454, 969, 1186
TseFI GTSAC 3 cut(s) 31, 262, 1698
TseI GCWGC 6 cut(s) 659, 974, 1007, 1295, 1546, 1758
Tsp45I GTSAC 3 cut(s) 31, 262, 1698
TspDTI ATGAA 6 cut(s) 20, 175, 387, 507, 573, 854
TspGWI ACGGA 1 cut(s) 1547
TspRI CASTG 3 cut(s) 454, 969, 1186
Vha464I CTTAAG 2 cut(s) 877, 1637
XapI RAATTY 2 cut(s) 54, 1669
XceI RCATGY 3 cut(s) 932, 1195, 1514
XcmI CCANNNNNNNNNTGG 2 cut(s) 961, 994
XmiI GTMKAC 1 cut(s) 1728
XspI CTAG 2 cut(s) 41, 1724
ZrmI AGTACT 1 cut(s) 1309
Zsp2I ATGCAT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.