Prupe.1G554000_v2.0.a1

Domain of unknown function (DUF4149)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0010354
Physical Location & Seq
Forward (+)
45265793 .. 45268048
2256 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G554000.1

Sequence Viewer

Length: 1491 bp
ATGATGAATTTTGTGGCGGTCTTCCTGGTTGTGAGCTCGCTGGCCAGCGCTGGAATGTGGTCTCCGAGACCACAAAATGATAATCAGAACCAGGAGAAGAAGCAAGGAGATCATCAGAGGGAGGTTATAGTGAAAAATGGGCACAGGGTTGTAGTCGTGGAGTATGACGAGCATGGCCATCCCATCACCAAAGTCTCAATCTCATCGGAACAAGACCGTCAAGCCGACAAGGATTCTTCCATTTCCGACAATGACGTGTCGTCGCCCTCTATGGTCTCGAGGGCATTCGACAAAGCTATGGACAACATGAAGGAAGCGACCTCTGCTCTCCCCAACCTCGGCCAAGAGGGTCACAGCCCGAAAGAGCTTATATGCGACGCGTATGGAAAATGCAAGCACCAGATTGCAAGGGATCTCGAGATGAAGAAGAAGATTGTGAAGGGGACCGTGCACGGGGCGAAGGAGACGGCACATAAGGTCGGGGAGGCGGTGGAAAGTGCGTACGAGAAGACGGGCGAGGCGGTGGGGAATGTATATGAAAAGGCGAAAGAAATGGTGTCCGATAAAGCTCATGAGGTTGAGGAAACTGCCAAGGACTCGGTGGGGAAAGCGAAGGGCGCCGCTAAAACGGCCAGGGAAATGGGGGAGACCTTGGCCGCGGATATAAAGAGCAACGCGTCGAACCTGTGCGAAGACTTGGCAAGCAAAGCTCAAGAGGCCAAAGAGACTGCAGAGCATGCTGCTGCGAAAGTCAAGACTGGAGCAGAGGAGCTCGAGAGTGGGACCCAGAAGAGGTTTCATGAACTGGGTCGCAAAGGGAGAGAGATTTGGTACGGTGCCCTGCGGTCTACGGGATTGAGTGATGCTTTGGACGCTTTGATGTCTGTGGCTAATTTGTTTGGGTTGGCCACTGCTTATGGGACGTGCATGTGGATTACGTTCGTTTCAAGCCACGTGTTGTTGGGAAATTTGGCACCGCAGCAGTTTGGGATGGTCCAGAGCAAGATTTACAGGGTTTATTTCAGGGCCATGGCTTCTAGTGTTGGGATGGCGTTGTTGGGACATCTTTGGAGACACGGAAATAAAAAGTTCACAGGGAATATGTTGCAGTTGCAGAACTTTAATCTTATGGCTGCCCTGTTGATGATTTTTGTCAATATGTTATACTTGGAGCCTCGATCCACCAAGGTGATGTTTGAGAGAATGAAGATGGAGAAGGAAGAAGGAAGAGAAAGAGTACATGTTGGTACCAGAGTCAGCAGCGAGTCTGAGCAGCAACGAATTACCGCCGAAGTCGAACCCACAACCACGGTTGCCTCACAAGTCACCGAAAGAAGGGAGCAAGAAGTAGCTCAGGCTGATACTGATAGGCTCGACGAGATGCGCGAGAGGCTTAAGAAGCTAAACTCATATTCGTCGTTGCTTAACGTCATGTCGCTAATGTCTCTGAGCTGGCATCTCGTGTACCTCGCCCAGCGCCTGCACAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4149

Protein Analysis

497

Amino Acids

55.12

Weight (kDa)

7.01

Isoelectric Point (pI)

31.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22600 AT1G72100
fragaria_vesca FvH4_5g31970
malus_domestica MD15G1396400.v1.1 MD15G1407700.v1.1
prunus_persica Prupe.1G554000_v2.0.a1
pyrus_communis pycom15g36370
rosa_chinensis RchiOBHm_Chr7g0227991
rosa_laevigata RLG00000001601
rosa_multiflora Rmu_co8508245.1_g000001 Rmu_sc0006493.1_g000005
rosa_roxburghii Rroxscaffold_3G00231680
rosa_rugosa Rorug07G0244900
rosa_samantha Rh7AG398000 Rh7AG398300 Rh7AG398800 Rh7BG380100 Rh7CG416400 Rh7CG416900 Rh7DG394400
rosa_wichuraiana Rw7G033150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1247
AccB1I GGYRCC 4 cut(s) 617, 836, 973, 1247
AccI GTMKAC 1 cut(s) 848
AccII CGCG 4 cut(s) 380, 659, 677, 1386
AciI CCGC 9 cut(s) 17, 488, 521, 621, 657, 659, 844, 977, 1287
AclWI GGATC 2 cut(s) 420, 1173
AcoI YGGCCR 6 cut(s) 42, 175, 340, 630, 654, 906
AcsI RAATTY 2 cut(s) 7, 967
AcvI CACGTG 1 cut(s) 955
AcyI GRCGYC 1 cut(s) 618
AfaI GTAC 5 cut(s) 503, 833, 1239, 1249, 1466
AfeI AGCGCT 1 cut(s) 49
AfiI CCNNNNNNNGG 4 cut(s) 338, 453, 792, 1335
AflII CTTAAG 1 cut(s) 1394
AflIII ACRYGT 5 cut(s) 255, 378, 675, 954, 1240
AgsI TTSAA 1 cut(s) 948
AhdI GACNNNNNGTC 1 cut(s) 259
AjiI CACGTC 2 cut(s) 256, 924
AjnI CCWGG 3 cut(s) 24, 90, 632
AleI CACNNNNGTG 1 cut(s) 1187
AluBI AGCT 9 cut(s) 36, 296, 367, 569, 710, 772, 1352, 1402, 1452
AluI AGCT 9 cut(s) 36, 296, 367, 569, 710, 772, 1352, 1402, 1452
Alw21I GWGCWC 3 cut(s) 38, 453, 774
Alw26I GTCTC 9 cut(s) 61, 66, 199, 280, 458, 641, 719, 1066, 1449
Alw44I GTGCAC 1 cut(s) 449
AlwI GGATC 2 cut(s) 420, 1173
AlwNI CAGNNNCTG 1 cut(s) 1480
Ama87I CYCGRG 3 cut(s) 277, 416, 773
Aor51HI AGCGCT 1 cut(s) 49
AoxI GGCC 8 cut(s) 42, 175, 340, 630, 654, 717, 906, 1026
ApaLI GTGCAC 1 cut(s) 449
ApeKI GCWGC 6 cut(s) 740, 743, 979, 1133, 1260, 1273
ApoI RAATTY 2 cut(s) 7, 967
Asp718I GGTACC 1 cut(s) 1247
AspLEI GCGC 4 cut(s) 50, 620, 1386, 1479
AspS9I GGNCC 4 cut(s) 444, 783, 994, 1026
AsuHPI GGTGA 3 cut(s) 178, 1201, 1318
AvaI CYCGRG 3 cut(s) 277, 416, 773
AvaII GGWCC 3 cut(s) 444, 783, 994
BaeGI GKGCMC 3 cut(s) 144, 453, 841
BaeI ACNNNNGTAYC 2 cut(s) 1239, 1272
BalI TGGCCA 3 cut(s) 44, 177, 908
BanI GGYRCC 4 cut(s) 617, 836, 973, 1247
BanII GRGCYC 2 cut(s) 38, 774
BauI CACGAG 1 cut(s) 1460
BbrPI CACGTG 1 cut(s) 955
BbsI GAAGAC 3 cut(s) 13, 515, 699
Bbv12I GWGCWC 3 cut(s) 38, 453, 774
BbvI GCAGC 6 cut(s) 727, 730, 991, 1120, 1272, 1285
BccI CCATC 5 cut(s) 186, 191, 985, 1042, 1204
BceAI ACGGC 2 cut(s) 483, 645
BciT130I CCWGG 3 cut(s) 26, 92, 634
BcoDI GTCTC 9 cut(s) 61, 66, 199, 280, 458, 641, 719, 1066, 1449
BfaI CTAG 1 cut(s) 1038
BfmI CTRYAG 1 cut(s) 729
BfoI RGCGCY 3 cut(s) 51, 621, 1480
BfrI CTTAAG 1 cut(s) 1394
BisI GCNGC 8 cut(s) 621, 657, 741, 744, 980, 1134, 1261, 1274
BlsI GCNGC 8 cut(s) 622, 658, 742, 745, 981, 1135, 1262, 1275
Bme1390I CCNGG 3 cut(s) 26, 92, 634
Bme18I GGWCC 3 cut(s) 444, 783, 994
BmeRI GACNNNNNGTC 1 cut(s) 259
BmeT110I CYCGRG 3 cut(s) 277, 416, 773
BmgBI CACGTC 2 cut(s) 256, 924
BmgT120I GGNCC 4 cut(s) 444, 783, 994, 1026
BmiI GGNNCC 8 cut(s) 445, 619, 784, 785, 838, 975, 1173, 1249
BmrFI CCNGG 3 cut(s) 26, 92, 634
BmrI ACTGGG 1 cut(s) 815
BmsI GCATC 3 cut(s) 853, 1371, 1465
BmuI ACTGGG 1 cut(s) 815
BpiI GAAGAC 3 cut(s) 13, 515, 699
BpmI CTGGAG 1 cut(s) 780
Bpu10I CCTNAGC 1 cut(s) 1353
BpuEI CTTGAG 1 cut(s) 696
BsaAI YACGTR 1 cut(s) 955
BsaHI GRCGYC 1 cut(s) 618
BsaI GGTCTC 4 cut(s) 61, 66, 280, 641
BsaJI CCNNGG 8 cut(s) 337, 591, 633, 651, 657, 1029, 1185, 1308
Bsc4I CCNNNNNNNGG 4 cut(s) 338, 453, 792, 1335
Bse1I ACTGG 2 cut(s) 763, 810
BseBI CCWGG 3 cut(s) 26, 92, 634
BseDI CCNNGG 8 cut(s) 337, 591, 633, 651, 657, 1029, 1185, 1308
BseGI GGATG 3 cut(s) 178, 996, 1053
BseLI CCNNNNNNNGG 4 cut(s) 338, 453, 792, 1335
BseMII CTCAG 3 cut(s) 1260, 1367, 1439
BseNI ACTGG 2 cut(s) 763, 810
BseRI GAGGAG 1 cut(s) 782
BseSI GKGCMC 3 cut(s) 144, 453, 841
BseXI GCAGC 6 cut(s) 727, 730, 991, 1120, 1272, 1285
BseYI CCCAGC 1 cut(s) 1473
BsgI GTGCAG 1 cut(s) 1466
Bsh1236I CGCG 4 cut(s) 380, 659, 677, 1386
BshFI GGCC 8 cut(s) 44, 177, 342, 632, 656, 719, 908, 1028
BshNI GGYRCC 4 cut(s) 617, 836, 973, 1247
BsiHKAI GWGCWC 3 cut(s) 38, 453, 774
BsiHKCI CYCGRG 3 cut(s) 277, 416, 773
BsiWI CGTACG 1 cut(s) 501
BslFI GGGAC 4 cut(s) 457, 796, 934, 1074
BslI CCNNNNNNNGG 4 cut(s) 338, 453, 792, 1335
BsmAI GTCTC 9 cut(s) 61, 66, 199, 280, 458, 641, 719, 1066, 1449
BsmBI CGTCTC 1 cut(s) 458
BsmFI GGGAC 4 cut(s) 457, 796, 934, 1074
BsmI GAATGC 1 cut(s) 284
BsnI GGCC 8 cut(s) 44, 177, 342, 632, 656, 719, 908, 1028
Bso31I GGTCTC 4 cut(s) 61, 66, 280, 641
BsoBI CYCGRG 3 cut(s) 277, 416, 773
Bsp1286I GDGCHC 5 cut(s) 38, 144, 453, 774, 841
Bsp143I GATC 3 cut(s) 109, 412, 1178
Bsp19I CCATGG 1 cut(s) 1029
BspACI CCGC 9 cut(s) 17, 488, 521, 621, 657, 659, 844, 977, 1287
BspANI GGCC 8 cut(s) 44, 177, 342, 632, 656, 719, 908, 1028
BspCNI CTCAG 3 cut(s) 1261, 1366, 1440
BspFNI CGCG 4 cut(s) 380, 659, 677, 1386
BspHI TCATGA 2 cut(s) 571, 799
BspLI GGNNCC 8 cut(s) 445, 619, 784, 785, 838, 975, 1173, 1249
BspMAI CTGCAG 1 cut(s) 733
BspPI GGATC 2 cut(s) 420, 1173
BspT107I GGYRCC 4 cut(s) 617, 836, 973, 1247
BspTI CTTAAG 1 cut(s) 1394
BspTNI GGTCTC 4 cut(s) 61, 66, 280, 641
BsrI ACTGG 2 cut(s) 763, 810
BssECI CCNNGG 8 cut(s) 337, 591, 633, 651, 657, 1029, 1185, 1308
BssMI GATC 3 cut(s) 109, 412, 1178
BssNI GRCGYC 1 cut(s) 618
BssSI CACGAG 1 cut(s) 1460
BssT1I CCWWGG 4 cut(s) 591, 651, 1029, 1185
Bst2BI CACGAG 1 cut(s) 1460
Bst2UI CCWGG 3 cut(s) 26, 92, 634
Bst4CI ACNGT 5 cut(s) 218, 448, 836, 1312, 1487
Bst6I CTCTTC 2 cut(s) 785, 1222
BstACI GRCGYC 1 cut(s) 618
BstAFI CTTAAG 1 cut(s) 1394
BstAPI GCANNNNNTGC 1 cut(s) 737
BstBAI YACGTR 1 cut(s) 955
BstC8I GCNNGC 8 cut(s) 38, 42, 46, 395, 703, 738, 1454, 1481
BstDEI CTNAG 3 cut(s) 1269, 1353, 1448
BstDSI CCRYGG 3 cut(s) 657, 1029, 1308
BstF5I GGATG 3 cut(s) 178, 996, 1053
BstFNI CGCG 4 cut(s) 380, 659, 677, 1386
BstH2I RGCGCY 3 cut(s) 51, 621, 1480
BstHHI GCGC 4 cut(s) 50, 620, 1386, 1479
BstKTI GATC 3 cut(s) 112, 415, 1181
BstMAI GTCTC 9 cut(s) 61, 66, 199, 280, 458, 641, 719, 1066, 1449
BstMBI GATC 3 cut(s) 109, 412, 1178
BstMWI GCNNNNNNNGC 9 cut(s) 323, 617, 629, 707, 716, 737, 872, 1390, 1399
BstNI CCWGG 3 cut(s) 26, 92, 634
BstNSI RCATGY 3 cut(s) 740, 931, 1244
BstSCI CCNGG 3 cut(s) 24, 90, 632
BstSFI CTRYAG 1 cut(s) 729
BstSLI GKGCMC 3 cut(s) 144, 453, 841
BstUI CGCG 4 cut(s) 380, 659, 677, 1386
BstV1I GCAGC 6 cut(s) 727, 730, 991, 1120, 1272, 1285
BstV2I GAAGAC 3 cut(s) 13, 515, 699
BstX2I RGATCY 1 cut(s) 412
BstXI CCANNNNNNTGG 1 cut(s) 640
BstYI RGATCY 1 cut(s) 412
BsuRI GGCC 8 cut(s) 44, 177, 342, 632, 656, 719, 908, 1028
BtgI CCRYGG 3 cut(s) 657, 1029, 1308
BtrI CACGTC 2 cut(s) 256, 924
BtsCI GGATG 3 cut(s) 178, 996, 1053
BtsI GCAGTG 1 cut(s) 909
BtsIMutI CAGTG 1 cut(s) 909
Cac8I GCNNGC 8 cut(s) 38, 42, 46, 395, 703, 738, 1454, 1481
CaiI CAGNNNCTG 1 cut(s) 1480
CciI TCATGA 2 cut(s) 571, 799
CfoI GCGC 4 cut(s) 50, 620, 1386, 1479
Cfr13I GGNCC 4 cut(s) 444, 783, 994, 1026
Cfr42I CCGCGG 1 cut(s) 660
CseI GACGC 3 cut(s) 386, 666, 881
Csp6I GTAC 5 cut(s) 502, 832, 1238, 1248, 1465
CviAII CATG 9 cut(s) 173, 307, 572, 737, 800, 928, 1030, 1241, 1432
CviQI GTAC 5 cut(s) 502, 832, 1238, 1248, 1465
DdeI CTNAG 3 cut(s) 1269, 1353, 1448
DinI GGCGCC 1 cut(s) 619
DpnI GATC 3 cut(s) 111, 414, 1180
DpnII GATC 3 cut(s) 109, 412, 1178
DriI GACNNNNNGTC 1 cut(s) 259
EaeI YGGCCR 6 cut(s) 42, 175, 340, 630, 654, 906
Eam1104I CTCTTC 2 cut(s) 785, 1222
Eam1105I GACNNNNNGTC 1 cut(s) 259
EarI CTCTTC 2 cut(s) 785, 1222
Ecl136II GAGCTC 2 cut(s) 36, 772
Eco130I CCWWGG 4 cut(s) 591, 651, 1029, 1185
Eco24I GRGCYC 2 cut(s) 38, 774
Eco31I GGTCTC 4 cut(s) 61, 66, 280, 641
Eco47I GGWCC 3 cut(s) 444, 783, 994
Eco47III AGCGCT 1 cut(s) 49
Eco53kI GAGCTC 2 cut(s) 36, 772
Eco72I CACGTG 1 cut(s) 955
Eco88I CYCGRG 3 cut(s) 277, 416, 773
EcoICRI GAGCTC 2 cut(s) 36, 772
EcoO109I RGGNCCY 1 cut(s) 783
EcoRII CCWGG 3 cut(s) 24, 90, 632
EcoT14I CCWWGG 4 cut(s) 591, 651, 1029, 1185
EcoT38I GRGCYC 2 cut(s) 38, 774
EgeI GGCGCC 1 cut(s) 619
EheI GGCGCC 1 cut(s) 619
ErhI CCWWGG 4 cut(s) 591, 651, 1029, 1185
Esp3I CGTCTC 1 cut(s) 458
FaeI CATG 9 cut(s) 176, 310, 575, 740, 803, 931, 1033, 1244, 1435
FaqI GGGAC 4 cut(s) 457, 796, 934, 1074
FatI CATG 9 cut(s) 172, 306, 571, 736, 799, 927, 1029, 1240, 1431
FblI GTMKAC 1 cut(s) 848
Fnu4HI GCNGC 8 cut(s) 621, 657, 741, 744, 980, 1134, 1261, 1274
FokI GGATG 3 cut(s) 165, 1003, 1060
FriOI GRGCYC 2 cut(s) 38, 774
Fsp4HI GCNGC 8 cut(s) 621, 657, 741, 744, 980, 1134, 1261, 1274
FspBI CTAG 1 cut(s) 1038
GlaI GCGC 4 cut(s) 49, 619, 1385, 1478
GluI GCNGC 8 cut(s) 621, 657, 741, 744, 980, 1134, 1261, 1274
GsaI CCCAGC 1 cut(s) 1477
GsuI CTGGAG 1 cut(s) 780
HaeII RGCGCY 3 cut(s) 51, 621, 1480
HaeIII GGCC 8 cut(s) 44, 177, 342, 632, 656, 719, 908, 1028
HgaI GACGC 3 cut(s) 386, 666, 881
HhaI GCGC 4 cut(s) 50, 620, 1386, 1479
Hin1I GRCGYC 1 cut(s) 618
Hin1II CATG 9 cut(s) 176, 310, 575, 740, 803, 931, 1033, 1244, 1435
Hin6I GCGC 4 cut(s) 48, 618, 1384, 1477
HinP1I GCGC 4 cut(s) 48, 618, 1384, 1477
HinfI GANTC 4 cut(s) 233, 596, 1254, 1265
HphI GGTGA 3 cut(s) 178, 1201, 1318
Hpy166II GTNNAC 4 cut(s) 451, 849, 1092, 1465
Hpy188I TCNGA 8 cut(s) 66, 87, 117, 208, 247, 562, 1270, 1449
Hpy188III TCNNGA 9 cut(s) 277, 416, 418, 572, 713, 754, 775, 800, 997
Hpy8I GTNNAC 4 cut(s) 451, 849, 1092, 1465
Hpy99I CGWCG 5 cut(s) 265, 380, 682, 1379, 1420
HpyAV CCTTC 7 cut(s) 304, 433, 454, 607, 1210, 1217, 1329
HpyCH4III ACNGT 5 cut(s) 218, 448, 836, 1312, 1487
HpyCH4IV ACGT 5 cut(s) 255, 923, 938, 954, 1428
HpyCH4V TGCA 8 cut(s) 393, 407, 451, 731, 927, 1108, 1114, 1483
HpyF10VI GCNNNNNNNGC 9 cut(s) 323, 617, 629, 707, 716, 737, 872, 1390, 1399
HpyF3I CTNAG 3 cut(s) 1269, 1353, 1448
HpySE526I ACGT 5 cut(s) 255, 923, 938, 954, 1428
Hsp92I GRCGYC 1 cut(s) 618
Hsp92II CATG 9 cut(s) 176, 310, 575, 740, 803, 931, 1033, 1244, 1435
HspAI GCGC 4 cut(s) 48, 618, 1384, 1477
KasI GGCGCC 1 cut(s) 617
KflI GGGWCCC 1 cut(s) 783
KpnI GGTACC 1 cut(s) 1251
KspI CCGCGG 1 cut(s) 660
Kzo9I GATC 3 cut(s) 109, 412, 1178
LmnI GCTCC 4 cut(s) 761, 769, 1171, 1339
Lsp1109I GCAGC 6 cut(s) 727, 730, 991, 1120, 1272, 1285
LweI GCATC 3 cut(s) 853, 1371, 1465
MaeI CTAG 1 cut(s) 1038
MaeII ACGT 5 cut(s) 255, 923, 938, 954, 1428
MaeIII GTNAC 2 cut(s) 350, 1324
MalI GATC 3 cut(s) 111, 414, 1180
MboI GATC 3 cut(s) 109, 412, 1178
MflI RGATCY 1 cut(s) 412
MhlI GDGCHC 5 cut(s) 38, 144, 453, 774, 841
MlsI TGGCCA 3 cut(s) 44, 177, 908
MluCI AATT 4 cut(s) 7, 892, 967, 1281
MluI ACGCGT 2 cut(s) 378, 675
MluNI TGGCCA 3 cut(s) 44, 177, 908
Mly113I GGCGCC 1 cut(s) 618
MlyI GAGTC 3 cut(s) 590, 1263, 1274
MmeI TCCRAC 1 cut(s) 270
Mox20I TGGCCA 3 cut(s) 44, 177, 908
MscI TGGCCA 3 cut(s) 44, 177, 908
MseI TTAA 3 cut(s) 1122, 1395, 1425
MslI CAYNNNNRTG 1 cut(s) 1187
Msp20I TGGCCA 3 cut(s) 44, 177, 908
MspA1I CMGCKG 1 cut(s) 659
MspCI CTTAAG 1 cut(s) 1394
MspR9I CCNGG 3 cut(s) 26, 92, 634
Mva1269I GAATGC 1 cut(s) 284
MvaI CCWGG 3 cut(s) 26, 92, 634
MvnI CGCG 4 cut(s) 380, 659, 677, 1386
MwoI GCNNNNNNNGC 9 cut(s) 323, 617, 629, 707, 716, 737, 872, 1390, 1399
NarI GGCGCC 1 cut(s) 618
NcoI CCATGG 1 cut(s) 1029
NdeII GATC 3 cut(s) 109, 412, 1178
NlaIII CATG 9 cut(s) 176, 310, 575, 740, 803, 931, 1033, 1244, 1435
NlaIV GGNNCC 8 cut(s) 445, 619, 784, 785, 838, 975, 1173, 1249
NmeAIII GCCGAG 1 cut(s) 318
NmuCI GTSAC 2 cut(s) 350, 1324
NspI RCATGY 3 cut(s) 740, 931, 1244
OliI CACNNNNGTG 1 cut(s) 1187
PaeI GCATGC 1 cut(s) 740
PaeR7I CTCGAG 3 cut(s) 277, 416, 773
PagI TCATGA 2 cut(s) 571, 799
PciI ACATGT 1 cut(s) 1240
PcsI WCGNNNNNNNCGW 1 cut(s) 1383
PctI GAATGC 1 cut(s) 284
PfeI GAWTC 1 cut(s) 233
Pfl23II CGTACG 1 cut(s) 501
PkrI GCNGC 8 cut(s) 622, 658, 742, 745, 981, 1135, 1262, 1275
PleI GAGTC 3 cut(s) 590, 1262, 1273
PluTI GGCGCC 1 cut(s) 621
PmaCI CACGTG 1 cut(s) 955
PmlI CACGTG 1 cut(s) 955
PpsI GAGTC 3 cut(s) 590, 1262, 1273
Ppu21I YACGTR 1 cut(s) 955
PpuMI RGGWCCY 1 cut(s) 783
PscI ACATGT 1 cut(s) 1240
Psp124BI GAGCTC 2 cut(s) 38, 774
Psp5II RGGWCCY 1 cut(s) 783
Psp6I CCWGG 3 cut(s) 24, 90, 632
PspCI CACGTG 1 cut(s) 955
PspFI CCCAGC 1 cut(s) 1473
PspGI CCWGG 3 cut(s) 24, 90, 632
PspLI CGTACG 1 cut(s) 501
PspN4I GGNNCC 8 cut(s) 445, 619, 784, 785, 838, 975, 1173, 1249
PspPI GGNCC 4 cut(s) 444, 783, 994, 1026
PspPPI RGGWCCY 1 cut(s) 783
PstI CTGCAG 1 cut(s) 733
PstNI CAGNNNCTG 1 cut(s) 1480
PsuI RGATCY 1 cut(s) 412
RsaI GTAC 5 cut(s) 503, 833, 1239, 1249, 1466
RsaNI GTAC 5 cut(s) 502, 832, 1238, 1248, 1465
RseI CAYNNNNRTG 1 cut(s) 1187
SacI GAGCTC 2 cut(s) 38, 774
SacII CCGCGG 1 cut(s) 660
SaqAI TTAA 3 cut(s) 1122, 1395, 1425
SatI GCNGC 8 cut(s) 621, 657, 741, 744, 980, 1134, 1261, 1274
Sau3AI GATC 3 cut(s) 109, 412, 1178
Sau96I GGNCC 4 cut(s) 444, 783, 994, 1026
SchI GAGTC 3 cut(s) 590, 1263, 1274
ScrFI CCNGG 3 cut(s) 26, 92, 634
SduI GDGCHC 5 cut(s) 38, 144, 453, 774, 841
SfaNI GCATC 3 cut(s) 853, 1371, 1465
SfcI CTRYAG 1 cut(s) 729
SfoI GGCGCC 1 cut(s) 619
Sfr274I CTCGAG 3 cut(s) 277, 416, 773
Sfr303I CCGCGG 1 cut(s) 660
SgrBI CCGCGG 1 cut(s) 660
SinI GGWCC 3 cut(s) 444, 783, 994
SlaI CTCGAG 3 cut(s) 277, 416, 773
SmiMI CAYNNNNRTG 1 cut(s) 1187
SmlI CTYRAG 5 cut(s) 277, 416, 711, 773, 1394
SmoI CTYRAG 5 cut(s) 277, 416, 711, 773, 1394
SphI GCATGC 1 cut(s) 740
Sse9I AATT 4 cut(s) 7, 892, 967, 1281
SsiI CCGC 9 cut(s) 17, 488, 521, 621, 657, 659, 844, 977, 1287
SspDI GGCGCC 1 cut(s) 617
SspMI CTAG 1 cut(s) 1038
SstI GAGCTC 2 cut(s) 38, 774
StyD4I CCNGG 3 cut(s) 24, 90, 632
StyI CCWWGG 4 cut(s) 591, 651, 1029, 1185
TaaI ACNGT 5 cut(s) 218, 448, 836, 1312, 1487
TaiI ACGT 5 cut(s) 258, 926, 941, 957, 1431
TaqI TCGA 8 cut(s) 278, 288, 417, 680, 774, 1177, 1296, 1374
TasI AATT 4 cut(s) 7, 892, 967, 1281
TatI WGTACW 1 cut(s) 1237
TauI GCSGC 2 cut(s) 623, 659
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 3 cut(s) 1122, 1395, 1425
Tru9I TTAA 3 cut(s) 1122, 1395, 1425
TscAI CASTG 1 cut(s) 916
TseFI GTSAC 2 cut(s) 350, 1324
TseI GCWGC 6 cut(s) 740, 743, 979, 1133, 1260, 1273
Tsp45I GTSAC 2 cut(s) 350, 1324
TspDTI ATGAA 7 cut(s) 20, 323, 437, 552, 788, 816, 1220
TspGWI ACGGA 1 cut(s) 1092
TspRI CASTG 1 cut(s) 916
Vha464I CTTAAG 1 cut(s) 1394
VneI GTGCAC 1 cut(s) 449
VpaK11BI GGWCC 3 cut(s) 444, 783, 994
XapI RAATTY 2 cut(s) 7, 967
XceI RCATGY 3 cut(s) 740, 931, 1244
XcmI CCANNNNNNNNNTGG 1 cut(s) 598
XhoI CTCGAG 3 cut(s) 277, 416, 773
XmiI GTMKAC 1 cut(s) 848
XspI CTAG 1 cut(s) 1038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.