MD15G1396400.v1.1

Domain of unknown function (DUF4149)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010354
Physical Location & Seq
Reverse (-)
49591419 .. 49593493
2075 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1396400.v1.1.491

Sequence Viewer

Length: 651 bp
ATGCAGCCTCTTCTGGGTCCCACTTTTGAGCTCCTCCGCTCCAGCCTTAACCTTACCAGCTGCTTCCTCCGCAGTCTCTTTCGCTTCTTTCGCTTTTTCAGCCAAGTCACTTTTGAGCTCCTCCGCTGCAGCCTTCACCTTAAAAGCTGCTTCCTCCGTAGTCTCTTTAGCTTCTTTTGCTTTTTCCGCCAAGTCACTTTTGAGCTCCCCCGCTCCAGCCTTCACCTTACCAACTGCTTCCTCCGCAGTCTCCTTCCCTTCTTTCGCTTTTTTCGCCAAGTGTTCGCGCTGTCTCGCCTTCATTTTCAGTCTTGTTCTATCTGCAGTGTGATGTTTGAGAGAATGAAGATCGAGAAGGAAGAAGGAAGAGGAAGAGAGAGGGTACATGTTGATGCCAGAATCAGCAGGGAGGCTGGCCACCAGCAGCAGCAGCAGACTACCACTGAAGGTGAACACTCAATCACGACGCCTCCACAAGTCTCGAGGAGGGAGCAACAGGTGGTTGAGCCTGATATTGAGAGGCTTAACGAGATGCGTGAGAGGCTGAAGAAGCTAAATAAATTTTCTTCATTCCTCAACATCGCGTCACTAATGGCTCTTAGCTGGCATCTTGTGTATCTTGCCCAGCACCTCCCCAGTTCATGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4149

Protein Analysis

217

Amino Acids

25.6

Weight (kDa)

9.71

Isoelectric Point (pI)

61.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22600 AT1G72100
fragaria_vesca FvH4_5g31970
malus_domestica MD15G1396400.v1.1 MD15G1407700.v1.1
prunus_persica Prupe.1G554000_v2.0.a1
pyrus_communis pycom15g36370
rosa_chinensis RchiOBHm_Chr7g0227991
rosa_laevigata RLG00000001601
rosa_multiflora Rmu_co8508245.1_g000001 Rmu_sc0006493.1_g000005
rosa_roxburghii Rroxscaffold_3G00231680
rosa_rugosa Rorug07G0244900
rosa_samantha Rh7AG398000 Rh7AG398300 Rh7AG398800 Rh7BG380100 Rh7CG416400 Rh7CG416900 Rh7DG394400
rosa_wichuraiana Rw7G033150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 39, 213
AccII CGCG 2 cut(s) 287, 584
AciI CCGC 6 cut(s) 37, 70, 124, 187, 211, 244
AcoI YGGCCR 1 cut(s) 415
AcsI RAATTY 1 cut(s) 560
AcuI CTGAAG 2 cut(s) 465, 566
AcyI GRCGYC 1 cut(s) 467
AfaI GTAC 1 cut(s) 384
AfiI CCNNNNNNNGG 1 cut(s) 14
AflIII ACRYGT 1 cut(s) 385
AluBI AGCT 8 cut(s) 31, 60, 118, 147, 171, 205, 553, 603
AluI AGCT 8 cut(s) 31, 60, 118, 147, 171, 205, 553, 603
Alw21I GWGCWC 3 cut(s) 33, 120, 207
Alw26I GTCTC 5 cut(s) 80, 167, 254, 297, 484
Ama87I CYCGRG 1 cut(s) 481
AoxI GGCC 1 cut(s) 415
ApeKI GCWGC 8 cut(s) 4, 60, 126, 129, 147, 424, 427, 430
ApoI RAATTY 1 cut(s) 560
AspLEI GCGC 1 cut(s) 289
AspS9I GGNCC 1 cut(s) 17
AsuHPI GGTGA 3 cut(s) 128, 215, 461
AvaI CYCGRG 1 cut(s) 481
AvaII GGWCC 1 cut(s) 17
BalI TGGCCA 1 cut(s) 417
BanII GRGCYC 3 cut(s) 33, 120, 207
Bbv12I GWGCWC 3 cut(s) 33, 120, 207
BbvI GCAGC 8 cut(s) 16, 47, 113, 134, 141, 436, 439, 442
BcoDI GTCTC 5 cut(s) 80, 167, 254, 297, 484
BfmI CTRYAG 2 cut(s) 127, 322
BisI GCNGC 8 cut(s) 5, 61, 127, 130, 148, 425, 428, 431
BlsI GCNGC 8 cut(s) 6, 62, 128, 131, 149, 426, 429, 432
Bme18I GGWCC 1 cut(s) 17
BmeT110I CYCGRG 1 cut(s) 481
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 2 cut(s) 18, 19
BmrI ACTGGG 1 cut(s) 630
BmsI GCATC 3 cut(s) 382, 522, 616
BmuI ACTGGG 1 cut(s) 630
BpmI CTGGAG 2 cut(s) 25, 199
BsaHI GRCGYC 1 cut(s) 467
BsaXI ACNNNNNCTCC 2 cut(s) 454, 484
Bsc4I CCNNNNNNNGG 1 cut(s) 14
Bse1I ACTGG 1 cut(s) 636
BseLI CCNNNNNNNGG 1 cut(s) 14
BseNI ACTGG 1 cut(s) 636
BseRI GAGGAG 3 cut(s) 23, 110, 499
BseXI GCAGC 8 cut(s) 16, 47, 113, 134, 141, 436, 439, 442
BseYI CCCAGC 1 cut(s) 624
Bsh1236I CGCG 2 cut(s) 287, 584
BshFI GGCC 1 cut(s) 417
BsiHKAI GWGCWC 3 cut(s) 33, 120, 207
BsiHKCI CYCGRG 1 cut(s) 481
BslFI GGGAC 1 cut(s) 3
BslI CCNNNNNNNGG 1 cut(s) 14
BsmAI GTCTC 5 cut(s) 80, 167, 254, 297, 484
BsmFI GGGAC 1 cut(s) 3
BsnI GGCC 1 cut(s) 417
BsoBI CYCGRG 1 cut(s) 481
Bsp1286I GDGCHC 3 cut(s) 33, 120, 207
Bsp143I GATC 1 cut(s) 348
BspACI CCGC 6 cut(s) 37, 70, 124, 187, 211, 244
BspANI GGCC 1 cut(s) 417
BspFNI CGCG 2 cut(s) 287, 584
BspLI GGNNCC 2 cut(s) 18, 19
BspMAI CTGCAG 2 cut(s) 131, 326
BsrBI CCGCTC 2 cut(s) 39, 213
BsrI ACTGG 1 cut(s) 636
BssMI GATC 1 cut(s) 348
BssNI GRCGYC 1 cut(s) 467
Bst6I CTCTTC 3 cut(s) 15, 361, 367
BstACI GRCGYC 1 cut(s) 467
BstC8I GCNNGC 2 cut(s) 415, 605
BstDEI CTNAG 1 cut(s) 599
BstFNI CGCG 2 cut(s) 287, 584
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 1 cut(s) 351
BstMAI GTCTC 5 cut(s) 80, 167, 254, 297, 484
BstMBI GATC 1 cut(s) 348
BstNSI RCATGY 1 cut(s) 389
BstSFI CTRYAG 2 cut(s) 127, 322
BstUI CGCG 2 cut(s) 287, 584
BstV1I GCAGC 8 cut(s) 16, 47, 113, 134, 141, 436, 439, 442
BsuRI GGCC 1 cut(s) 417
BtgZI GCGATG 1 cut(s) 565
BtsI GCAGTG 1 cut(s) 331
BtsIMutI CAGTG 2 cut(s) 331, 441
Cac8I GCNNGC 2 cut(s) 415, 605
CfoI GCGC 1 cut(s) 289
Cfr13I GGNCC 1 cut(s) 17
CseI GACGC 2 cut(s) 475, 573
Csp6I GTAC 1 cut(s) 383
CviAII CATG 2 cut(s) 386, 642
CviQI GTAC 1 cut(s) 383
DdeI CTNAG 1 cut(s) 599
DpnI GATC 1 cut(s) 350
DpnII GATC 1 cut(s) 348
EaeI YGGCCR 1 cut(s) 415
Eam1104I CTCTTC 3 cut(s) 15, 361, 367
EarI CTCTTC 3 cut(s) 15, 361, 367
EciI GGCGGA 1 cut(s) 176
Ecl136II GAGCTC 3 cut(s) 31, 118, 205
Eco24I GRGCYC 3 cut(s) 33, 120, 207
Eco47I GGWCC 1 cut(s) 17
Eco53kI GAGCTC 3 cut(s) 31, 118, 205
Eco57I CTGAAG 2 cut(s) 465, 566
Eco88I CYCGRG 1 cut(s) 481
EcoICRI GAGCTC 3 cut(s) 31, 118, 205
EcoO109I RGGNCCY 1 cut(s) 17
EcoT38I GRGCYC 3 cut(s) 33, 120, 207
FaeI CATG 2 cut(s) 389, 645
FaiI YATR 2 cut(s) 387, 643
FaqI GGGAC 1 cut(s) 3
FatI CATG 2 cut(s) 385, 641
FauI CCCGC 1 cut(s) 218
Fnu4HI GCNGC 8 cut(s) 5, 61, 127, 130, 148, 425, 428, 431
FriOI GRGCYC 3 cut(s) 33, 120, 207
Fsp4HI GCNGC 8 cut(s) 5, 61, 127, 130, 148, 425, 428, 431
GlaI GCGC 1 cut(s) 288
GluI GCNGC 8 cut(s) 5, 61, 127, 130, 148, 425, 428, 431
GsaI CCCAGC 1 cut(s) 628
GsuI CTGGAG 2 cut(s) 25, 199
HaeIII GGCC 1 cut(s) 417
HgaI GACGC 2 cut(s) 475, 573
HhaI GCGC 1 cut(s) 289
Hin1I GRCGYC 1 cut(s) 467
Hin1II CATG 2 cut(s) 389, 645
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HinfI GANTC 1 cut(s) 399
HphI GGTGA 3 cut(s) 128, 215, 461
Hpy166II GTNNAC 1 cut(s) 452
Hpy188III TCNNGA 3 cut(s) 352, 463, 481
Hpy8I GTNNAC 1 cut(s) 452
Hpy99I CGWCG 1 cut(s) 469
HpyAV CCTTC 8 cut(s) 143, 230, 263, 268, 308, 349, 356, 440
HpyCH4V TGCA 3 cut(s) 4, 129, 324
HpyF3I CTNAG 1 cut(s) 599
Hsp92I GRCGYC 1 cut(s) 467
Hsp92II CATG 2 cut(s) 389, 645
HspAI GCGC 1 cut(s) 287
KflI GGGWCCC 1 cut(s) 17
Kzo9I GATC 1 cut(s) 348
LmnI GCTCC 6 cut(s) 36, 44, 123, 210, 218, 490
Lsp1109I GCAGC 8 cut(s) 16, 47, 113, 134, 141, 436, 439, 442
LweI GCATC 3 cut(s) 382, 522, 616
MaeIII GTNAC 3 cut(s) 106, 193, 585
MalI GATC 1 cut(s) 350
MbiI CCGCTC 2 cut(s) 39, 213
MboI GATC 1 cut(s) 348
MboII GAAGA 6 cut(s) 358, 371, 378, 384, 558, 559
MhlI GDGCHC 3 cut(s) 33, 120, 207
MlsI TGGCCA 1 cut(s) 417
MluCI AATT 1 cut(s) 560
MluNI TGGCCA 1 cut(s) 417
Mox20I TGGCCA 1 cut(s) 417
MscI TGGCCA 1 cut(s) 417
MseI TTAA 3 cut(s) 48, 141, 525
MslI CAYNNNNRTG 1 cut(s) 390
Msp20I TGGCCA 1 cut(s) 417
MspA1I CMGCKG 2 cut(s) 60, 126
MvnI CGCG 2 cut(s) 287, 584
NdeII GATC 1 cut(s) 348
NlaIII CATG 2 cut(s) 389, 645
NlaIV GGNNCC 2 cut(s) 18, 19
NmuCI GTSAC 3 cut(s) 106, 193, 585
NspI RCATGY 1 cut(s) 389
PaeR7I CTCGAG 1 cut(s) 481
PciI ACATGT 1 cut(s) 385
PfeI GAWTC 1 cut(s) 399
PkrI GCNGC 8 cut(s) 6, 62, 128, 131, 149, 426, 429, 432
PpuMI RGGWCCY 1 cut(s) 17
PscI ACATGT 1 cut(s) 385
Psp124BI GAGCTC 3 cut(s) 33, 120, 207
Psp5II RGGWCCY 1 cut(s) 17
PspFI CCCAGC 1 cut(s) 624
PspN4I GGNNCC 2 cut(s) 18, 19
PspPI GGNCC 1 cut(s) 17
PspPPI RGGWCCY 1 cut(s) 17
PstI CTGCAG 2 cut(s) 131, 326
PvuII CAGCTG 1 cut(s) 60
RsaI GTAC 1 cut(s) 384
RsaNI GTAC 1 cut(s) 383
RseI CAYNNNNRTG 1 cut(s) 390
SacI GAGCTC 3 cut(s) 33, 120, 207
SaqAI TTAA 3 cut(s) 48, 141, 525
SatI GCNGC 8 cut(s) 5, 61, 127, 130, 148, 425, 428, 431
Sau3AI GATC 1 cut(s) 348
Sau96I GGNCC 1 cut(s) 17
SduI GDGCHC 3 cut(s) 33, 120, 207
SfaNI GCATC 3 cut(s) 382, 522, 616
SfcI CTRYAG 2 cut(s) 127, 322
Sfr274I CTCGAG 1 cut(s) 481
SinI GGWCC 1 cut(s) 17
SlaI CTCGAG 1 cut(s) 481
SmiMI CAYNNNNRTG 1 cut(s) 390
SmlI CTYRAG 1 cut(s) 481
SmoI CTYRAG 1 cut(s) 481
Sse9I AATT 1 cut(s) 560
SsiI CCGC 6 cut(s) 37, 70, 124, 187, 211, 244
SstI GAGCTC 3 cut(s) 33, 120, 207
TaqI TCGA 2 cut(s) 351, 482
TasI AATT 1 cut(s) 560
TfiI GAWTC 1 cut(s) 399
Tru1I TTAA 3 cut(s) 48, 141, 525
Tru9I TTAA 3 cut(s) 48, 141, 525
TscAI CASTG 2 cut(s) 331, 448
TseFI GTSAC 3 cut(s) 106, 193, 585
TseI GCWGC 8 cut(s) 4, 60, 126, 129, 147, 424, 427, 430
Tsp45I GTSAC 3 cut(s) 106, 193, 585
TspDTI ATGAA 4 cut(s) 290, 359, 558, 630
TspGWI ACGGA 1 cut(s) 146
TspRI CASTG 2 cut(s) 331, 448
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 1 cut(s) 560
XceI RCATGY 1 cut(s) 389
XhoI CTCGAG 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.