AT1G25500

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007935
Physical Location & Seq
Reverse (-)
8955195 .. 8958132
2938 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G25500.3

Sequence Viewer

Length: 921 bp
ATGATGAAAGTTTCGGTTCACATCTTAACAACCTATCTAGCTGTAGTTAGTGTACTATGTTTCTGGTGCAGACTGTTCTTTTGGGGCGGTGCATTCGCTGTTGGATCTCTGTTGCAGTTCTTGTATGTTATTTCAGTTATAGACAGACTTCCTTTCACAATGCTGGTCCTACGGAAAGCGCTGAAATTAGTCTGGGGACTTCCTAAGGTGATAATGGTGGCACATGCTTTCACAGTAGTCATGCTTTTGTGGATGTCTCTATGGTCTTTTGGAGCAGCTGGTGTTGTGGCATCGAGCATGGGCGATGAGGGACGATGGTGGCTTCTTGTGGTTCTTTCGGTTAGCTTATTCTGGACAGGCGCTGTGCTATGTAACACTGTCCATGTTATAGTTTCTGGGATGGTGTTTCATGTTCTCTTTCACTGTGGTCAAGAAGAGTCATCATCGTTGCCTCCTAGCTCTTTGGTAGACTCACTTAGGTATGCCGTGACAACATCTTTTGGGAGCATATGTTACGGTTCACTCTTCACTGCTGCCATTAGAACACTTCGCTGGGAGATAAGGGGATTCCGGTCCAAGATTTGTGGAAACGAATGTTTACTTTGCTGCGTTGATTTCCTGTTTCATCTAGTTGAAACACTAGTTCGTTTTTTTAACAAGTATGCATACGTCCAGATTGCAGTGTATGGAAAAGGATTCAACAAATCAGCAAGAGACGCATGGGAATTGTTTCAGTCGACAGGTGTAGAGGCACTTGTTGCATATGACTGCTCTGGTGCTGTACTTCTGATGGGAACCATCTTTGGAGGACTCATCACAGGCTCTTGCATTGGTATATGGGCTTGGATCAAGTACAGTGACCGAGTTATAATGGTTGCATCTACGGCTATGTTGATGGGAATGGTTCTTGAATGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

306

Amino Acids

34.12

Weight (kDa)

8.63

Isoelectric Point (pI)

31.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 100 - 394 3.9e-59 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 869
AccI GTMKAC 2 cut(s) 468, 737
AciI CCGC 1 cut(s) 87
AclWI GGATC 2 cut(s) 112, 854
AfaI GTAC 3 cut(s) 54, 783, 854
AfeI AGCGCT 1 cut(s) 180
AgsI TTSAA 3 cut(s) 635, 700, 911
AhlI ACTAGT 1 cut(s) 640
AluBI AGCT 4 cut(s) 41, 278, 345, 459
AluI AGCT 4 cut(s) 41, 278, 345, 459
Alw26I GTCTC 2 cut(s) 261, 708
AlwI GGATC 2 cut(s) 112, 854
AlwNI CAGNNNCTG 1 cut(s) 362
Aor51HI AGCGCT 1 cut(s) 180
ApeKI GCWGC 3 cut(s) 275, 533, 606
ArsI GACNNNNNNTTYG 2 cut(s) 63, 95
Asp700I GAANNNNTTC 1 cut(s) 729
AspLEI GCGC 2 cut(s) 181, 362
AspS9I GGNCC 2 cut(s) 166, 573
AsuHPI GGTGA 1 cut(s) 220
AvaII GGWCC 2 cut(s) 166, 573
AxyI CCTNAGG 1 cut(s) 204
BbvI GCAGC 3 cut(s) 287, 520, 593
BccI CCATC 5 cut(s) 309, 394, 784, 806, 889
BceAI ACGGC 2 cut(s) 470, 900
BcoDI GTCTC 2 cut(s) 261, 708
BcuI ACTAGT 1 cut(s) 640
BfaI CTAG 4 cut(s) 38, 456, 629, 641
BfmI CTRYAG 1 cut(s) 42
BfoI RGCGCY 2 cut(s) 182, 363
BisI GCNGC 3 cut(s) 276, 534, 607
BlsI GCNGC 3 cut(s) 277, 535, 608
Bme18I GGWCC 2 cut(s) 166, 573
BmgT120I GGNCC 2 cut(s) 166, 573
BmiI GGNNCC 1 cut(s) 796
BmsI GCATC 2 cut(s) 299, 887
BsaWI WCCGGW 1 cut(s) 570
BsaXI ACNNNNNCTCC 2 cut(s) 496, 526
Bse21I CCTNAGG 1 cut(s) 204
BseGI GGATG 2 cut(s) 258, 405
BseXI GCAGC 3 cut(s) 287, 520, 593
BseYI CCCAGC 1 cut(s) 552
BsgI GTGCAG 1 cut(s) 88
BsiSI CCGG 1 cut(s) 571
BslFI GGGAC 2 cut(s) 210, 324
BsmAI GTCTC 2 cut(s) 261, 708
BsmBI CGTCTC 1 cut(s) 708
BsmFI GGGAC 2 cut(s) 210, 324
BsmI GAATGC 1 cut(s) 92
Bsp143I GATC 2 cut(s) 104, 846
BspACI CCGC 1 cut(s) 87
BspLI GGNNCC 1 cut(s) 796
BspPI GGATC 2 cut(s) 112, 854
BssMI GATC 2 cut(s) 104, 846
Bst4CI ACNGT 6 cut(s) 75, 235, 379, 425, 518, 857
Bst6I CTCTTC 2 cut(s) 429, 530
BstAPI GCANNNNNTGC 1 cut(s) 758
BstDEI CTNAG 2 cut(s) 204, 476
BstF5I GGATG 2 cut(s) 258, 405
BstH2I RGCGCY 2 cut(s) 182, 363
BstHHI GCGC 2 cut(s) 181, 362
BstKTI GATC 2 cut(s) 107, 849
BstMAI GTCTC 2 cut(s) 261, 708
BstMBI GATC 2 cut(s) 104, 846
BstMWI GCNNNNNNNGC 3 cut(s) 716, 758, 884
BstNSI RCATGY 1 cut(s) 227
BstSFI CTRYAG 1 cut(s) 42
BstV1I GCAGC 3 cut(s) 287, 520, 593
BstX2I RGATCY 1 cut(s) 104
BstYI RGATCY 1 cut(s) 104
Bsu36I CCTNAGG 1 cut(s) 204
BtgZI GCGATG 1 cut(s) 318
BtsCI GGATG 2 cut(s) 258, 405
BtsI GCAGTG 2 cut(s) 528, 687
BtsIMutI CAGTG 5 cut(s) 375, 421, 528, 687, 862
CaiI CAGNNNCTG 1 cut(s) 362
CfoI GCGC 2 cut(s) 181, 362
Cfr13I GGNCC 2 cut(s) 166, 573
CseI GACGC 1 cut(s) 725
Csp6I GTAC 3 cut(s) 53, 782, 853
CspCI CAANNNNNGTGG 2 cut(s) 565, 600
CviAII CATG 6 cut(s) 224, 241, 298, 383, 410, 720
CviJI RGCY 8 cut(s) 41, 278, 322, 345, 459, 822, 842, 887
CviKI_1 RGCY 8 cut(s) 41, 278, 322, 345, 459, 822, 842, 887
CviQI GTAC 3 cut(s) 53, 782, 853
DdeI CTNAG 2 cut(s) 204, 476
DpnI GATC 2 cut(s) 106, 848
DpnII GATC 2 cut(s) 104, 846
Eam1104I CTCTTC 2 cut(s) 429, 530
EarI CTCTTC 2 cut(s) 429, 530
Eco47I GGWCC 2 cut(s) 166, 573
Eco47III AGCGCT 1 cut(s) 180
Eco81I CCTNAGG 1 cut(s) 204
EcoT22I ATGCAT 1 cut(s) 667
Esp3I CGTCTC 1 cut(s) 708
FaeI CATG 6 cut(s) 227, 244, 301, 386, 413, 723
FaqI GGGAC 2 cut(s) 210, 324
FatI CATG 6 cut(s) 223, 240, 297, 382, 409, 719
FauNDI CATATG 2 cut(s) 509, 763
FblI GTMKAC 2 cut(s) 468, 737
Fnu4HI GCNGC 3 cut(s) 276, 534, 607
FokI GGATG 2 cut(s) 265, 412
Fsp4HI GCNGC 3 cut(s) 276, 534, 607
FspBI CTAG 4 cut(s) 38, 456, 629, 641
GlaI GCGC 2 cut(s) 180, 361
GluI GCNGC 3 cut(s) 276, 534, 607
GsaI CCCAGC 1 cut(s) 556
HaeII RGCGCY 2 cut(s) 182, 363
HapII CCGG 1 cut(s) 571
HgaI GACGC 1 cut(s) 725
HhaI GCGC 2 cut(s) 181, 362
Hin1II CATG 6 cut(s) 227, 244, 301, 386, 413, 723
Hin6I GCGC 2 cut(s) 179, 360
HinP1I GCGC 2 cut(s) 179, 360
HincII GTYRAC 1 cut(s) 738
HindII GTYRAC 1 cut(s) 738
HinfI GANTC 5 cut(s) 437, 470, 567, 696, 810
HpaII CCGG 1 cut(s) 571
HphI GGTGA 1 cut(s) 220
Hpy166II GTNNAC 6 cut(s) 19, 53, 469, 521, 599, 738
Hpy188I TCNGA 1 cut(s) 789
Hpy188III TCNNGA 4 cut(s) 352, 431, 673, 908
Hpy8I GTNNAC 6 cut(s) 19, 53, 469, 521, 599, 738
HpyCH4III ACNGT 6 cut(s) 75, 235, 379, 425, 518, 857
HpyCH4IV ACGT 1 cut(s) 669
HpyCH4V TGCA 8 cut(s) 69, 92, 115, 665, 680, 761, 828, 878
HpyF10VI GCNNNNNNNGC 3 cut(s) 716, 758, 884
HpyF3I CTNAG 2 cut(s) 204, 476
HpySE526I ACGT 1 cut(s) 669
Hsp92II CATG 6 cut(s) 227, 244, 301, 386, 413, 723
HspAI GCGC 2 cut(s) 179, 360
Kzo9I GATC 2 cut(s) 104, 846
LmnI GCTCC 2 cut(s) 272, 504
Lsp1109I GCAGC 3 cut(s) 287, 520, 593
LweI GCATC 2 cut(s) 299, 887
MaeI CTAG 4 cut(s) 38, 456, 629, 641
MaeII ACGT 1 cut(s) 669
MaeIII GTNAC 4 cut(s) 371, 487, 512, 857
MalI GATC 2 cut(s) 106, 848
MboI GATC 2 cut(s) 104, 846
MboII GAAGA 2 cut(s) 446, 517
MflI RGATCY 1 cut(s) 104
MluCI AATT 2 cut(s) 185, 725
MlyI GAGTC 3 cut(s) 446, 464, 804
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 4 cut(s) 301, 462, 742, 800
Mph1103I ATGCAT 1 cut(s) 667
MroXI GAANNNNTTC 1 cut(s) 729
MseI TTAA 2 cut(s) 26, 654
MspA1I CMGCKG 1 cut(s) 278
MspI CCGG 1 cut(s) 571
Mva1269I GAATGC 1 cut(s) 92
MwoI GCNNNNNNNGC 3 cut(s) 716, 758, 884
NdeI CATATG 2 cut(s) 509, 763
NdeII GATC 2 cut(s) 104, 846
NlaIII CATG 6 cut(s) 227, 244, 301, 386, 413, 723
NlaIV GGNNCC 1 cut(s) 796
NmuCI GTSAC 2 cut(s) 487, 857
NsiI ATGCAT 1 cut(s) 667
NspI RCATGY 1 cut(s) 227
PctI GAATGC 1 cut(s) 92
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 2 cut(s) 567, 696
PkrI GCNGC 3 cut(s) 277, 535, 608
PleI GAGTC 3 cut(s) 445, 464, 804
PpsI GAGTC 3 cut(s) 445, 464, 804
PsiI TTATAA 1 cut(s) 869
PspFI CCCAGC 1 cut(s) 552
PspN4I GGNNCC 1 cut(s) 796
PspPI GGNCC 2 cut(s) 166, 573
PstNI CAGNNNCTG 1 cut(s) 362
PsuI RGATCY 1 cut(s) 104
PvuII CAGCTG 1 cut(s) 278
RsaI GTAC 3 cut(s) 54, 783, 854
RsaNI GTAC 3 cut(s) 53, 782, 853
SalI GTCGAC 1 cut(s) 736
SaqAI TTAA 2 cut(s) 26, 654
SatI GCNGC 3 cut(s) 276, 534, 607
Sau3AI GATC 2 cut(s) 104, 846
Sau96I GGNCC 2 cut(s) 166, 573
SchI GAGTC 3 cut(s) 446, 464, 804
SetI ASST 9 cut(s) 35, 43, 210, 280, 347, 461, 482, 672, 745
SfaNI GCATC 2 cut(s) 299, 887
SfcI CTRYAG 1 cut(s) 42
SinI GGWCC 2 cut(s) 166, 573
SpeI ACTAGT 1 cut(s) 640
Sse9I AATT 2 cut(s) 185, 725
SsiI CCGC 1 cut(s) 87
SspMI CTAG 4 cut(s) 38, 456, 629, 641
TaaI ACNGT 6 cut(s) 75, 235, 379, 425, 518, 857
TaiI ACGT 1 cut(s) 672
TaqI TCGA 2 cut(s) 293, 737
TaqII GACCGA 1 cut(s) 876
TasI AATT 2 cut(s) 185, 725
TatI WGTACW 3 cut(s) 52, 781, 852
TfiI GAWTC 2 cut(s) 567, 696
Tru1I TTAA 2 cut(s) 26, 654
Tru9I TTAA 2 cut(s) 26, 654
TscAI CASTG 5 cut(s) 382, 428, 535, 687, 862
TseFI GTSAC 2 cut(s) 487, 857
TseI GCWGC 3 cut(s) 275, 533, 606
Tsp45I GTSAC 2 cut(s) 487, 857
TspDTI ATGAA 3 cut(s) 20, 398, 614
TspGWI ACGGA 1 cut(s) 187
TspRI CASTG 5 cut(s) 382, 428, 535, 687, 862
VpaK11BI GGWCC 2 cut(s) 166, 573
XceI RCATGY 1 cut(s) 227
XmiI GTMKAC 2 cut(s) 468, 737
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 4 cut(s) 38, 456, 629, 641
Zsp2I ATGCAT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.