Prupe.1G307200_v2.0.a1

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0007935
Physical Location & Seq
Forward (+)
29999629 .. 30004762
5134 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G307200.3

Sequence Viewer

Length: 1512 bp
ATGAACATCTCGCAGAGCTCCTATTCTTCCTCGTCCTCCTCCGTCAATTCGGCCTCCGTCGCCACCGCCGATCCGAATGTGATCTCAAGTCGGAGGAGTGCAGACAGTAGTCAAACCCTGGCTTCTGAGCCGTCGCGTCGTTGGCGTGATATTTTTTGGTTGGGAGTATTTCTAATTCAATTGGTTTTAATGGGATGTGCTCTTACGGTTCTCGGGCTCAATAGGTTTAAGAAAACAGATAGGCTAAACATAGATAGGTACACCCAGCGTTTTTTGGAAAATCAAAGGGGATTGACGGAGAACTATTGGCCGCTTTATGCTCTTGCCGGTGGAGTTGGGACTGTTCTTGGTTGGACTTGGTTGTTATTCCTCAGTTCTCAAGCGAATCACATGATGAAGGTTGCGGTTCACATCTTGACTACTTATCTTGCTGTGATTAGCGTTCTATTATTTTGGATGGAACAGTTTTTCTGGGGCGTTGCATTTGCGATTGGTGCTGTGTTGCAGTTTTTGTATGTTATTTCAGTAATTGACAGACTTCCATTTACCATGCTGGTGCTACAAGCAGCTGTCAAGATGGTATGGAGTTTACGTGAAGTTATGAGAGTAGCATATGCATTCATGCTTATTATGCTTCTATGGATGGGAATATGGTCCTTTGGGGCAGCTGGTGTTGTAGCTTCAAGCATTGGAGATGGTGGACGTTGGTGGCTTCTTGTGGTTCTCTCTATAAGCTTATTTTGGACGGGTGCTGTACTCTGTAATACTGTACATGTTATAGTTTCTGGGATGGTGTTTCTTGTACTTATCCATGGTGGTAGAGAGGCATCATCAATGCCTCCGAACTCACTGATGAAGTCTCTTCGGTATGCAGTCACAACTTCTTTTGGCAGCATCTGCTATGGTTCACTTTTCACAGCTGCCATTCGGACACTTCGGTGGAAGATCAGGGGACTCCGATCCAAGATTGGCAACAATGAGTGTCTACTTTGCTGTGTTGATTTCCTCTTTCATCTAGTGGAGACTCTTGTCCGTTTCTTTAACAAGTATGCCTATGTCCAGATAGCTGTTTCTGGAAAAAGTTTTAATCATTCAGCGAGGGATGCCTGGGAGTTATTTCAGTCTACAGGAGTGGAGGCACTCGTTGCCTATGATTGTTCAGGTGCTGTTTTGTTGATGGGCACTCTATTGGGTGGACTTATCACTGGTACTTGCTCAGGGATCTGGACATGGATTAAATATAGGGACAGGGTGGTTATGGTGGGGTCCACTGCAATGCTGATGGGAATGGTCTTGGTGGGACTGGCAATGGTGGTTGTGGAAAGTGCTGTTACATCTATTTATATCTGCTATGCAGAAGACCCCTTGTTAATTCATAGGTGGGATGCTGAGTTCTTCCACCAGATGTCAGAGATGCTACACCAGCGTCTTCAACATAGGAGCTCGCGGGCGAGGGAAGTGTTAACTCACCACCGCTTTGATAACCACACACAAGAAACACTCCCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

504

Amino Acids

56.37

Weight (kDa)

9.2

Isoelectric Point (pI)

40.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 985, 1124
AccII CGCG 2 cut(s) 136, 1447
AciI CCGC 5 cut(s) 66, 311, 404, 1447, 1474
AclWI GGATC 3 cut(s) 65, 954, 1229
AcoI YGGCCR 1 cut(s) 308
AfaI GTAC 5 cut(s) 260, 756, 771, 804, 1210
AflIII ACRYGT 1 cut(s) 772
AgsI TTSAA 3 cut(s) 179, 684, 1433
AjnI CCWGG 2 cut(s) 117, 1106
AleI CACNNNNGTG 1 cut(s) 937
AloI GAACNNNNNNTCC 2 cut(s) 1376, 1408
AluBI AGCT 8 cut(s) 18, 569, 668, 680, 735, 920, 1067, 1443
AluI AGCT 8 cut(s) 18, 569, 668, 680, 735, 920, 1067, 1443
Alw21I GWGCWC 3 cut(s) 20, 202, 1445
Alw26I GTCTC 2 cut(s) 864, 1016
AlwI GGATC 3 cut(s) 65, 954, 1229
AlwNI CAGNNNCTG 2 cut(s) 897, 1166
Ama87I CYCGRG 1 cut(s) 212
AoxI GGCC 2 cut(s) 51, 308
ApeKI GCWGC 4 cut(s) 566, 665, 891, 920
AspS9I GGNCC 2 cut(s) 654, 1266
AsuHPI GGTGA 1 cut(s) 1460
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 2 cut(s) 654, 1266
BaeGI GKGCMC 1 cut(s) 1184
BanII GRGCYC 3 cut(s) 20, 219, 1445
BbsI GAAGAC 2 cut(s) 1365, 1421
Bbv12I GWGCWC 3 cut(s) 20, 202, 1445
BbvI GCAGC 4 cut(s) 578, 677, 903, 907
BccI CCATC 7 cut(s) 451, 571, 637, 689, 784, 1171, 1276
BceAI ACGGC 1 cut(s) 115
BciT130I CCWGG 2 cut(s) 119, 1108
BcoDI GTCTC 2 cut(s) 864, 1016
BfaI CTAG 1 cut(s) 1016
BfmI CTRYAG 1 cut(s) 1125
BisI GCNGC 5 cut(s) 311, 567, 666, 892, 921
BlsI GCNGC 5 cut(s) 312, 568, 667, 893, 922
Bme1390I CCNGG 2 cut(s) 119, 1108
Bme18I GGWCC 2 cut(s) 654, 1266
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 2 cut(s) 654, 1266
BmiI GGNNCC 1 cut(s) 1267
BmrFI CCNGG 2 cut(s) 119, 1108
BmsI GCATC 5 cut(s) 836, 903, 1093, 1375, 1404
BoxI GACNNNNGTC 2 cut(s) 108, 1028
BpiI GAAGAC 2 cut(s) 1365, 1421
Bpu10I CCTNAGC 1 cut(s) 1216
BpuEI CTTGAG 2 cut(s) 70, 363
BsaAI YACGTR 1 cut(s) 593
BsaJI CCNNGG 3 cut(s) 117, 811, 1107
BsaXI ACNNNNNCTCC 2 cut(s) 577, 607
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 2 cut(s) 1210, 1308
Bse3DI GCAATG 2 cut(s) 1281, 1314
BseBI CCWGG 2 cut(s) 119, 1108
BseDI CCNNGG 3 cut(s) 117, 811, 1107
BseGI GGATG 6 cut(s) 200, 462, 648, 795, 1108, 1390
BseMI GCAATG 2 cut(s) 1281, 1314
BseMII CTCAG 4 cut(s) 117, 385, 1230, 1380
BseNI ACTGG 2 cut(s) 1210, 1308
BseRI GAGGAG 2 cut(s) 28, 109
BseSI GKGCMC 1 cut(s) 1184
BseXI GCAGC 4 cut(s) 578, 677, 903, 907
BseYI CCCAGC 1 cut(s) 264
BsgI GTGCAG 1 cut(s) 120
Bsh1236I CGCG 2 cut(s) 136, 1447
BshFI GGCC 2 cut(s) 53, 310
BsiHKAI GWGCWC 3 cut(s) 20, 202, 1445
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 1 cut(s) 327
BslFI GGGAC 4 cut(s) 352, 966, 1259, 1314
BsmAI GTCTC 2 cut(s) 864, 1016
BsmFI GGGAC 4 cut(s) 352, 966, 1259, 1314
BsmI GAATGC 1 cut(s) 617
BsnI GGCC 2 cut(s) 53, 310
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 5 cut(s) 20, 202, 219, 1184, 1445
Bsp1407I TGTACA 1 cut(s) 769
Bsp143I GATC 5 cut(s) 70, 81, 945, 959, 1221
Bsp19I CCATGG 1 cut(s) 811
BspACI CCGC 5 cut(s) 66, 311, 404, 1447, 1474
BspANI GGCC 2 cut(s) 53, 310
BspCNI CTCAG 4 cut(s) 118, 384, 1229, 1381
BspFNI CGCG 2 cut(s) 136, 1447
BspLI GGNNCC 1 cut(s) 1267
BspPI GGATC 3 cut(s) 65, 954, 1229
BsrDI GCAATG 2 cut(s) 1281, 1314
BsrFI RCCGGY 1 cut(s) 326
BsrGI TGTACA 1 cut(s) 769
BsrI ACTGG 2 cut(s) 1210, 1308
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 3 cut(s) 117, 811, 1107
BssMI GATC 5 cut(s) 70, 81, 945, 959, 1221
BssT1I CCWWGG 1 cut(s) 811
Bst2UI CCWGG 2 cut(s) 119, 1108
Bst4CI ACNGT 5 cut(s) 107, 208, 343, 465, 769
Bst6I CTCTTC 1 cut(s) 867
BstAPI GCANNNNNTGC 2 cut(s) 897, 1145
BstAUI TGTACA 1 cut(s) 769
BstBAI YACGTR 1 cut(s) 593
BstC8I GCNNGC 2 cut(s) 1445, 1449
BstDEI CTNAG 4 cut(s) 126, 371, 1216, 1389
BstDSI CCRYGG 1 cut(s) 811
BstF5I GGATG 6 cut(s) 200, 462, 648, 795, 1108, 1390
BstFNI CGCG 2 cut(s) 136, 1447
BstKTI GATC 5 cut(s) 73, 84, 948, 962, 1224
BstMAI GTCTC 2 cut(s) 864, 1016
BstMBI GATC 5 cut(s) 70, 81, 945, 959, 1221
BstMWI GCNNNNNNNGC 8 cut(s) 59, 142, 494, 631, 897, 1103, 1145, 1423
BstNI CCWGG 2 cut(s) 119, 1108
BstNSI RCATGY 1 cut(s) 776
BstPAI GACNNNNGTC 2 cut(s) 108, 1028
BstSCI CCNGG 2 cut(s) 117, 1106
BstSFI CTRYAG 1 cut(s) 1125
BstSLI GKGCMC 1 cut(s) 1184
BstUI CGCG 2 cut(s) 136, 1447
BstV1I GCAGC 4 cut(s) 578, 677, 903, 907
BstV2I GAAGAC 2 cut(s) 1365, 1421
BstX2I RGATCY 1 cut(s) 1221
BstYI RGATCY 1 cut(s) 1221
BsuRI GGCC 2 cut(s) 53, 310
BtgI CCRYGG 1 cut(s) 811
BtsCI GGATG 6 cut(s) 200, 462, 648, 795, 1108, 1390
BtsI GCAGTG 1 cut(s) 1269
BtsIMutI CAGTG 3 cut(s) 848, 1203, 1269
Cac8I GCNNGC 2 cut(s) 1445, 1449
CaiI CAGNNNCTG 2 cut(s) 897, 1166
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 2 cut(s) 654, 1266
CseI GACGC 2 cut(s) 125, 1415
Csp6I GTAC 5 cut(s) 259, 755, 770, 803, 1209
CviAII CATG 6 cut(s) 391, 550, 622, 773, 812, 1230
CviQI GTAC 5 cut(s) 259, 755, 770, 803, 1209
DdeI CTNAG 4 cut(s) 126, 371, 1216, 1389
DpnI GATC 5 cut(s) 72, 83, 947, 961, 1223
DpnII GATC 5 cut(s) 70, 81, 945, 959, 1221
EaeI YGGCCR 1 cut(s) 308
Eam1104I CTCTTC 1 cut(s) 867
EarI CTCTTC 1 cut(s) 867
Ecl136II GAGCTC 2 cut(s) 18, 1443
Eco130I CCWWGG 1 cut(s) 811
Eco24I GRGCYC 3 cut(s) 20, 219, 1445
Eco47I GGWCC 2 cut(s) 654, 1266
Eco53kI GAGCTC 2 cut(s) 18, 1443
Eco88I CYCGRG 1 cut(s) 212
EcoICRI GAGCTC 2 cut(s) 18, 1443
EcoRII CCWGG 2 cut(s) 117, 1106
EcoT14I CCWWGG 1 cut(s) 811
EcoT22I ATGCAT 1 cut(s) 619
EcoT38I GRGCYC 3 cut(s) 20, 219, 1445
ErhI CCWWGG 1 cut(s) 811
FaeI CATG 6 cut(s) 394, 553, 625, 776, 815, 1233
FaqI GGGAC 4 cut(s) 352, 966, 1259, 1314
FatI CATG 6 cut(s) 390, 549, 621, 772, 811, 1229
FauI CCCGC 1 cut(s) 1440
FauNDI CATATG 1 cut(s) 613
FblI GTMKAC 2 cut(s) 985, 1124
Fnu4HI GCNGC 5 cut(s) 311, 567, 666, 892, 921
FokI GGATG 6 cut(s) 207, 469, 655, 802, 1115, 1397
FriOI GRGCYC 3 cut(s) 20, 219, 1445
Fsp4HI GCNGC 5 cut(s) 311, 567, 666, 892, 921
FspBI CTAG 1 cut(s) 1016
GluI GCNGC 5 cut(s) 311, 567, 666, 892, 921
GsaI CCCAGC 1 cut(s) 268
HaeIII GGCC 2 cut(s) 53, 310
HapII CCGG 1 cut(s) 327
HgaI GACGC 2 cut(s) 125, 1415
Hin1II CATG 6 cut(s) 394, 553, 625, 776, 815, 1233
HincII GTYRAC 1 cut(s) 1464
HindII GTYRAC 1 cut(s) 1464
HindIII AAGCTT 1 cut(s) 733
HinfI GANTC 3 cut(s) 385, 954, 1024
HpaI GTTAAC 1 cut(s) 1464
HpaII CCGG 1 cut(s) 327
HphI GGTGA 1 cut(s) 1460
Hpy188I TCNGA 7 cut(s) 75, 93, 127, 843, 930, 959, 1411
Hpy188III TCNNGA 5 cut(s) 415, 574, 1060, 1074, 1225
Hpy99I CGWCG 3 cut(s) 62, 136, 141
HpyAV CCTTC 1 cut(s) 391
HpyCH4III ACNGT 5 cut(s) 107, 208, 343, 465, 769
HpyCH4IV ACGT 2 cut(s) 592, 703
HpyCH4V TGCA 7 cut(s) 101, 482, 505, 617, 872, 1274, 1355
HpyF10VI GCNNNNNNNGC 8 cut(s) 59, 142, 494, 631, 897, 1103, 1145, 1423
HpyF3I CTNAG 4 cut(s) 126, 371, 1216, 1389
HpySE526I ACGT 2 cut(s) 592, 703
Hsp92II CATG 6 cut(s) 394, 553, 625, 776, 815, 1233
KspAI GTTAAC 1 cut(s) 1464
Kzo9I GATC 5 cut(s) 70, 81, 945, 959, 1221
LmnI GCTCC 2 cut(s) 23, 1440
Lsp1109I GCAGC 4 cut(s) 578, 677, 903, 907
LweI GCATC 5 cut(s) 836, 903, 1093, 1375, 1404
MaeI CTAG 1 cut(s) 1016
MaeII ACGT 2 cut(s) 592, 703
MaeIII GTNAC 2 cut(s) 874, 1330
MalI GATC 5 cut(s) 72, 83, 947, 961, 1223
MboI GATC 5 cut(s) 70, 81, 945, 959, 1221
MboII GAAGA 6 cut(s) 18, 854, 955, 1370, 1387, 1421
MfeI CAATTG 1 cut(s) 179
MflI RGATCY 1 cut(s) 1221
MhlI GDGCHC 5 cut(s) 20, 202, 219, 1184, 1445
MluCI AATT 5 cut(s) 46, 174, 179, 528, 1371
MlyI GAGTC 2 cut(s) 948, 1018
MmeI TCCRAC 2 cut(s) 71, 332
Mph1103I ATGCAT 1 cut(s) 619
MseI TTAA 7 cut(s) 188, 228, 1041, 1086, 1236, 1370, 1463
MslI CAYNNNNRTG 3 cut(s) 554, 937, 1274
MspA1I CMGCKG 3 cut(s) 569, 668, 920
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 2 cut(s) 119, 1108
MunI CAATTG 1 cut(s) 179
Mva1269I GAATGC 1 cut(s) 617
MvaI CCWGG 2 cut(s) 119, 1108
MvnI CGCG 2 cut(s) 136, 1447
MwoI GCNNNNNNNGC 8 cut(s) 59, 142, 494, 631, 897, 1103, 1145, 1423
NcoI CCATGG 1 cut(s) 811
NdeI CATATG 1 cut(s) 613
NdeII GATC 5 cut(s) 70, 81, 945, 959, 1221
NlaIII CATG 6 cut(s) 394, 553, 625, 776, 815, 1233
NlaIV GGNNCC 1 cut(s) 1267
NmuCI GTSAC 1 cut(s) 874
NsiI ATGCAT 1 cut(s) 619
NspI RCATGY 1 cut(s) 776
OliI CACNNNNGTG 1 cut(s) 937
PciI ACATGT 1 cut(s) 772
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 1 cut(s) 617
PfeI GAWTC 1 cut(s) 385
PkrI GCNGC 5 cut(s) 312, 568, 667, 893, 922
PleI GAGTC 2 cut(s) 948, 1018
PpsI GAGTC 2 cut(s) 948, 1018
Ppu21I YACGTR 1 cut(s) 593
PscI ACATGT 1 cut(s) 772
PshAI GACNNNNGTC 2 cut(s) 108, 1028
Psp124BI GAGCTC 2 cut(s) 20, 1445
Psp6I CCWGG 2 cut(s) 117, 1106
PspFI CCCAGC 1 cut(s) 264
PspGI CCWGG 2 cut(s) 117, 1106
PspN4I GGNNCC 1 cut(s) 1267
PspPI GGNCC 2 cut(s) 654, 1266
PstNI CAGNNNCTG 2 cut(s) 897, 1166
PsuI RGATCY 1 cut(s) 1221
PvuII CAGCTG 3 cut(s) 569, 668, 920
RsaI GTAC 5 cut(s) 260, 756, 771, 804, 1210
RsaNI GTAC 5 cut(s) 259, 755, 770, 803, 1209
RseI CAYNNNNRTG 3 cut(s) 554, 937, 1274
SacI GAGCTC 2 cut(s) 20, 1445
SaqAI TTAA 7 cut(s) 188, 228, 1041, 1086, 1236, 1370, 1463
SatI GCNGC 5 cut(s) 311, 567, 666, 892, 921
Sau3AI GATC 5 cut(s) 70, 81, 945, 959, 1221
Sau96I GGNCC 2 cut(s) 654, 1266
SchI GAGTC 2 cut(s) 948, 1018
ScrFI CCNGG 2 cut(s) 119, 1108
SduI GDGCHC 5 cut(s) 20, 202, 219, 1184, 1445
SfaNI GCATC 5 cut(s) 836, 903, 1093, 1375, 1404
SfcI CTRYAG 1 cut(s) 1125
SinI GGWCC 2 cut(s) 654, 1266
SmiMI CAYNNNNRTG 3 cut(s) 554, 937, 1274
SmlI CTYRAG 2 cut(s) 85, 378
SmoI CTYRAG 2 cut(s) 85, 378
Sse9I AATT 5 cut(s) 46, 174, 179, 528, 1371
SsiI CCGC 5 cut(s) 66, 311, 404, 1447, 1474
SspMI CTAG 1 cut(s) 1016
SstI GAGCTC 2 cut(s) 20, 1445
StyD4I CCNGG 2 cut(s) 117, 1106
StyI CCWWGG 1 cut(s) 811
TaaI ACNGT 5 cut(s) 107, 208, 343, 465, 769
TaiI ACGT 2 cut(s) 595, 706
TasI AATT 5 cut(s) 46, 174, 179, 528, 1371
TatI WGTACW 3 cut(s) 754, 769, 802
TauI GCSGC 1 cut(s) 313
TfiI GAWTC 1 cut(s) 385
Tru1I TTAA 7 cut(s) 188, 228, 1041, 1086, 1236, 1370, 1463
Tru9I TTAA 7 cut(s) 188, 228, 1041, 1086, 1236, 1370, 1463
TscAI CASTG 3 cut(s) 855, 1210, 1276
TseFI GTSAC 1 cut(s) 874
TseI GCWGC 4 cut(s) 566, 665, 891, 920
Tsp45I GTSAC 1 cut(s) 874
TspDTI ATGAA 6 cut(s) 17, 410, 610, 869, 1001, 1364
TspGWI ACGGA 4 cut(s) 31, 46, 311, 1022
TspRI CASTG 3 cut(s) 855, 1210, 1276
VpaK11BI GGWCC 2 cut(s) 654, 1266
XceI RCATGY 1 cut(s) 776
XmiI GTMKAC 2 cut(s) 985, 1124
XspI CTAG 1 cut(s) 1016
Zsp2I ATGCAT 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.