FvH4_4g27760

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007935
Physical Location & Seq
Reverse (-)
28597854 .. 28603844
5991 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27760.t3

Sequence Viewer

Length: 1509 bp
ATGAACAACGTCTCTCACAGCTCCTCCTCTTCCTCGTCCGTCAACTCCGCCTCCATTTCCGCCTCCGATCCGAATGTGGGCCGGAGCCGGAGTGGCGACGGCAGCCAAACCATGGCGGCGGAGCCGTCACGGCGGTGGCGTGATGTGTTCTGGTTGGGGATATTTATGATTCATTTGATTTTAGTGGGAGGTGCTCTGGCGGTTTTGGGTCTCAATAGGTTTAAGAAATCGGATAGGCTGAACTTGGATCAGTACACAAAGAATTACTTGGAAAATCAGAAAGGATTTACTGAGGATTATTGGCCGCTTTATGCTCTTGCTGGTGGAGTTGGGACTGTTCTTGGTTGGACTTGGTTGTTGTTCCTTGGATCTCAGGCGAGTCATATGATGAAGATTGCGGTGCATATCTTGACGACTTATCTTGCTGTGCTCAGTGTTCTGTGTTTTTGGATGGAGCAGTTCTTCTGGGGAGTTGCATTTGCGATCGGCGCTGGGTTGTTGTTCTTGTATGTGTTGGCAGTCATAGACAGACTACCCTTTACCATGCTGGTGCTACAAGCAGCTGTCAAGATGGTATGGAGTCTACCTGAAGTTATGAGAGTAGCATATGCATTCATGCTTATTATGCTTTTATGGATGGGAATATGGTCCTTTGGGGCTGCTGGTGTTGTGGCTTTAGGCATTGGAGATGGTGGACGCTGGTGGCTTCTTGTGGTTCTCTCTGTAAGCTTATTTTGGACGGGTGCTGTTCTCTGTAATACTGTACATGTTATAGTGTCTGGGATGGTGTTTCTTGTTCTTTCCCATGGTGGTCGCGAGGCCTCATCAATACCTTCAAAGTCACTAATGAAGTCTCTTCGGTATGCAGTCACAACTTCTTTTGGCAGCATCTGCTACGGTTCACTCTTCACAGCTGCCATTCGATCTTTGCGCTGGAAGATCAGGGGAGTTCGGTCCAAGATTGGTAACAATGAGTGTCTACTTTGCTGTGTCGATTTCCTCTTTCATCTTGTGGAGACACTTGTGCGATTCTTTAACAAGTATGCCTATGTTCAGATAGCTGTTTCTGGCAAAAGTTTCAATCATTCAGCGAGGGATGCTTGGGAGTTATTTCAGTCTACTGGAGTCGAGGCCCTCATTGCCTATGATTGCTCAGGTGCTGTATTATTGATGGGCACACTCTTGGGTGGGCTTATTACCGGAACTTGCTCAGGCGTCTGGACATGGGTTAAATATAGGGACAGAGTGGCCATGGTTGGTTCCACTGCAATGTTGATGGGAATGATCTTGGTGGGGATAGCAATGGTGGTTGTGGAAAGTGCTGTTACATCTATCTATATTTGTTATGCAGAAGACCCGTTATTAATTCATAGGTGGGATCCCGAATTCTTCAACCAGATGTCGGAGATGCTTCACCAGCGTCTTCAACACAGGAGTGCCCGGGCAAGGGAAGTGTTAACCCACAATCGGGTTGATAACCACCACACTCTCGAAACGCTCCCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

503

Amino Acids

55.75

Weight (kDa)

9.0

Isoelectric Point (pI)

35.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 159 - 230 7.6e-07 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 112
AccI GTMKAC 3 cut(s) 583, 979, 1118
AccII CGCG 1 cut(s) 816
AciI CCGC 8 cut(s) 48, 60, 116, 119, 133, 200, 305, 398
AclWI GGATC 5 cut(s) 62, 255, 376, 1373, 1386
AcoI YGGCCR 2 cut(s) 302, 1248
AcsI RAATTY 1 cut(s) 1385
AcuI CTGAAG 1 cut(s) 609
AcyI GRCGYC 1 cut(s) 1215
AfaI GTAC 2 cut(s) 254, 765
AfiI CCNNNNNNNGG 7 cut(s) 77, 112, 1402, 1446, 1447, 1467, 1468
AflIII ACRYGT 1 cut(s) 766
AgsI TTSAA 4 cut(s) 837, 1081, 1393, 1427
AleI CACNNNNGTG 2 cut(s) 133, 1434
AluBI AGCT 5 cut(s) 21, 563, 729, 914, 1061
AluI AGCT 5 cut(s) 21, 563, 729, 914, 1061
Alw21I GWGCWC 2 cut(s) 196, 432
Alw26I GTCTC 4 cut(s) 16, 215, 858, 1010
AlwI GGATC 5 cut(s) 62, 255, 376, 1373, 1386
AlwNI CAGNNNCTG 2 cut(s) 891, 1160
Ama87I CYCGRG 1 cut(s) 1440
AoxI GGCC 5 cut(s) 79, 302, 819, 1131, 1248
ApeKI GCWGC 5 cut(s) 102, 560, 659, 885, 914
ApoI RAATTY 1 cut(s) 1385
AseI ATTAAT 1 cut(s) 1364
AspLEI GCGC 2 cut(s) 491, 933
AspS9I GGNCC 4 cut(s) 79, 648, 954, 1132
AsuC2I CCSGG 2 cut(s) 1441, 1442
AsuHPI GGTGA 1 cut(s) 1406
AvaI CYCGRG 1 cut(s) 1440
AvaII GGWCC 2 cut(s) 648, 954
BaeGI GKGCMC 2 cut(s) 1178, 1441
BalI TGGCCA 1 cut(s) 1250
BamHI GGATCC 1 cut(s) 1378
BbsI GAAGAC 2 cut(s) 1359, 1415
Bbv12I GWGCWC 2 cut(s) 196, 432
BbvI GCAGC 5 cut(s) 114, 572, 646, 897, 901
BccI CCATC 7 cut(s) 445, 565, 631, 683, 778, 1165, 1270
BceAI ACGGC 3 cut(s) 109, 115, 146
BcnI CCSGG 2 cut(s) 1441, 1442
BcoDI GTCTC 4 cut(s) 16, 215, 858, 1010
BfoI RGCGCY 1 cut(s) 492
BglI GCCNNNNNGGC 2 cut(s) 93, 130
BisI GCNGC 7 cut(s) 103, 117, 305, 561, 660, 886, 915
BlsI GCNGC 7 cut(s) 104, 118, 306, 562, 661, 887, 916
Bme1390I CCNGG 2 cut(s) 1441, 1442
Bme18I GGWCC 2 cut(s) 648, 954
BmeT110I CYCGRG 1 cut(s) 1440
BmgT120I GGNCC 4 cut(s) 79, 648, 954, 1132
BmiI GGNNCC 4 cut(s) 86, 123, 1261, 1380
BmrFI CCNGG 2 cut(s) 1441, 1442
BmsI GCATC 3 cut(s) 897, 1087, 1398
BpiI GAAGAC 2 cut(s) 1359, 1415
BpmI CTGGAG 1 cut(s) 1143
Bpu10I CCTNAGC 2 cut(s) 1153, 1210
BpuMI CCSGG 2 cut(s) 1441, 1442
BsaHI GRCGYC 1 cut(s) 1215
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 5 cut(s) 111, 364, 805, 1251, 1440
BsaWI WCCGGW 1 cut(s) 1199
BsaXI ACNNNNNCTCC 6 cut(s) 8, 35, 38, 65, 76, 106
Bsc4I CCNNNNNNNGG 7 cut(s) 77, 112, 1402, 1446, 1447, 1467, 1468
Bse1I ACTGG 1 cut(s) 1126
Bse3DI GCAATG 3 cut(s) 1137, 1275, 1308
BseDI CCNNGG 5 cut(s) 111, 364, 805, 1251, 1440
BseGI GGATG 4 cut(s) 456, 642, 789, 1102
BseLI CCNNNNNNNGG 7 cut(s) 77, 112, 1402, 1446, 1447, 1467, 1468
BseMI GCAATG 3 cut(s) 1137, 1275, 1308
BseMII CTCAG 5 cut(s) 282, 386, 445, 1167, 1224
BseNI ACTGG 1 cut(s) 1126
BseRI GAGGAG 2 cut(s) 13, 16
BseSI GKGCMC 2 cut(s) 1178, 1441
BseXI GCAGC 5 cut(s) 114, 572, 646, 897, 901
BseYI CCCAGC 1 cut(s) 491
Bsh1236I CGCG 1 cut(s) 816
Bsh1285I CGRYCG 1 cut(s) 486
BshFI GGCC 5 cut(s) 81, 304, 821, 1133, 1250
BsiEI CGRYCG 1 cut(s) 486
BsiHKAI GWGCWC 2 cut(s) 196, 432
BsiHKCI CYCGRG 1 cut(s) 1440
BsiSI CCGG 4 cut(s) 82, 88, 1200, 1441
BslFI GGGAC 2 cut(s) 346, 1253
BslI CCNNNNNNNGG 7 cut(s) 77, 112, 1402, 1446, 1447, 1467, 1468
BsmAI GTCTC 4 cut(s) 16, 215, 858, 1010
BsmBI CGTCTC 1 cut(s) 16
BsmFI GGGAC 2 cut(s) 346, 1253
BsmI GAATGC 1 cut(s) 611
BsnI GGCC 5 cut(s) 81, 304, 821, 1133, 1250
Bso31I GGTCTC 1 cut(s) 215
BsoBI CYCGRG 1 cut(s) 1440
Bsp1286I GDGCHC 4 cut(s) 196, 432, 1178, 1441
Bsp1407I TGTACA 1 cut(s) 763
Bsp143I GATC 8 cut(s) 67, 247, 368, 483, 923, 939, 1284, 1378
Bsp19I CCATGG 3 cut(s) 111, 805, 1251
Bsp68I TCGCGA 1 cut(s) 816
BspACI CCGC 8 cut(s) 48, 60, 116, 119, 133, 200, 305, 398
BspANI GGCC 5 cut(s) 81, 304, 821, 1133, 1250
BspCNI CTCAG 5 cut(s) 283, 385, 444, 1166, 1223
BspFNI CGCG 1 cut(s) 816
BspLI GGNNCC 4 cut(s) 86, 123, 1261, 1380
BspPI GGATC 5 cut(s) 62, 255, 376, 1373, 1386
BspTNI GGTCTC 1 cut(s) 215
BsrDI GCAATG 3 cut(s) 1137, 1275, 1308
BsrGI TGTACA 1 cut(s) 763
BsrI ACTGG 1 cut(s) 1126
BssECI CCNNGG 5 cut(s) 111, 364, 805, 1251, 1440
BssMI GATC 8 cut(s) 67, 247, 368, 483, 923, 939, 1284, 1378
BssNI GRCGYC 1 cut(s) 1215
BssT1I CCWWGG 4 cut(s) 111, 364, 805, 1251
Bst4CI ACNGT 3 cut(s) 337, 763, 899
Bst6I CTCTTC 3 cut(s) 34, 861, 911
BstACI GRCGYC 1 cut(s) 1215
BstAPI GCANNNNNTGC 1 cut(s) 891
BstAUI TGTACA 1 cut(s) 763
BstDEI CTNAG 5 cut(s) 291, 372, 431, 1153, 1210
BstDSI CCRYGG 3 cut(s) 111, 805, 1251
BstF5I GGATG 4 cut(s) 456, 642, 789, 1102
BstFNI CGCG 1 cut(s) 816
BstH2I RGCGCY 1 cut(s) 492
BstHHI GCGC 2 cut(s) 491, 933
BstKTI GATC 8 cut(s) 70, 250, 371, 486, 926, 942, 1287, 1381
BstMAI GTCTC 4 cut(s) 16, 215, 858, 1010
BstMBI GATC 8 cut(s) 67, 247, 368, 483, 923, 939, 1284, 1378
BstMCI CGRYCG 1 cut(s) 486
BstMWI GCNNNNNNNGC 9 cut(s) 93, 102, 130, 488, 625, 891, 1097, 1139, 1417
BstNSI RCATGY 1 cut(s) 770
BstSCI CCNGG 2 cut(s) 1439, 1440
BstSLI GKGCMC 2 cut(s) 1178, 1441
BstUI CGCG 1 cut(s) 816
BstV1I GCAGC 5 cut(s) 114, 572, 646, 897, 901
BstV2I GAAGAC 2 cut(s) 1359, 1415
BstX2I RGATCY 2 cut(s) 368, 1378
BstYI RGATCY 2 cut(s) 368, 1378
BsuRI GGCC 5 cut(s) 81, 304, 821, 1133, 1250
BtgI CCRYGG 3 cut(s) 111, 805, 1251
BtsCI GGATG 4 cut(s) 456, 642, 789, 1102
BtsI GCAGTG 1 cut(s) 1263
BtsIMutI CAGTG 2 cut(s) 439, 1263
BtuMI TCGCGA 1 cut(s) 816
CaiI CAGNNNCTG 2 cut(s) 891, 1160
CfoI GCGC 2 cut(s) 491, 933
Cfr13I GGNCC 4 cut(s) 79, 648, 954, 1132
Cfr9I CCCGGG 1 cut(s) 1440
CseI GACGC 3 cut(s) 705, 1204, 1409
Csp6I GTAC 2 cut(s) 253, 764
CviAII CATG 7 cut(s) 112, 544, 616, 767, 806, 1224, 1252
CviQI GTAC 2 cut(s) 253, 764
DdeI CTNAG 5 cut(s) 291, 372, 431, 1153, 1210
DpnI GATC 8 cut(s) 69, 249, 370, 485, 925, 941, 1286, 1380
DpnII GATC 8 cut(s) 67, 247, 368, 483, 923, 939, 1284, 1378
EaeI YGGCCR 2 cut(s) 302, 1248
Eam1104I CTCTTC 3 cut(s) 34, 861, 911
EarI CTCTTC 3 cut(s) 34, 861, 911
EciI GGCGGA 3 cut(s) 37, 49, 134
Eco130I CCWWGG 4 cut(s) 111, 364, 805, 1251
Eco147I AGGCCT 1 cut(s) 821
Eco31I GGTCTC 1 cut(s) 215
Eco47I GGWCC 2 cut(s) 648, 954
Eco57I CTGAAG 1 cut(s) 609
Eco88I CYCGRG 1 cut(s) 1440
EcoO109I RGGNCCY 1 cut(s) 1132
EcoRI GAATTC 1 cut(s) 1385
EcoT14I CCWWGG 4 cut(s) 111, 364, 805, 1251
EcoT22I ATGCAT 1 cut(s) 613
ErhI CCWWGG 4 cut(s) 111, 364, 805, 1251
Esp3I CGTCTC 1 cut(s) 16
FaeI CATG 7 cut(s) 115, 547, 619, 770, 809, 1227, 1255
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FaqI GGGAC 2 cut(s) 346, 1253
FatI CATG 7 cut(s) 111, 543, 615, 766, 805, 1223, 1251
FauNDI CATATG 2 cut(s) 384, 607
FblI GTMKAC 3 cut(s) 583, 979, 1118
Fnu4HI GCNGC 7 cut(s) 103, 117, 305, 561, 660, 886, 915
FokI GGATG 4 cut(s) 463, 649, 796, 1109
Fsp4HI GCNGC 7 cut(s) 103, 117, 305, 561, 660, 886, 915
GlaI GCGC 2 cut(s) 490, 932
GluI GCNGC 7 cut(s) 103, 117, 305, 561, 660, 886, 915
GsaI CCCAGC 1 cut(s) 495
GsuI CTGGAG 1 cut(s) 1143
HaeII RGCGCY 1 cut(s) 492
HaeIII GGCC 5 cut(s) 81, 304, 821, 1133, 1250
HapII CCGG 4 cut(s) 82, 88, 1200, 1441
HgaI GACGC 3 cut(s) 705, 1204, 1409
HhaI GCGC 2 cut(s) 491, 933
Hin1I GRCGYC 1 cut(s) 1215
Hin1II CATG 7 cut(s) 115, 547, 619, 770, 809, 1227, 1255
Hin6I GCGC 2 cut(s) 489, 931
HinP1I GCGC 2 cut(s) 489, 931
HincII GTYRAC 2 cut(s) 43, 1458
HindII GTYRAC 2 cut(s) 43, 1458
HindIII AAGCTT 1 cut(s) 727
HinfI GANTC 5 cut(s) 169, 379, 580, 1029, 1125
HpaI GTTAAC 1 cut(s) 1458
HpaII CCGG 4 cut(s) 82, 88, 1200, 1441
HphI GGTGA 1 cut(s) 1406
Hpy166II GTNNAC 8 cut(s) 43, 255, 584, 695, 902, 980, 1119, 1458
Hpy188I TCNGA 6 cut(s) 67, 72, 232, 279, 1056, 1405
Hpy188III TCNNGA 6 cut(s) 409, 568, 815, 1219, 1382, 1490
Hpy8I GTNNAC 8 cut(s) 43, 255, 584, 695, 902, 980, 1119, 1458
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 1 cut(s) 843
HpyCH4III ACNGT 3 cut(s) 337, 763, 899
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 6 cut(s) 403, 476, 611, 866, 1268, 1349
HpyF10VI GCNNNNNNNGC 9 cut(s) 93, 102, 130, 488, 625, 891, 1097, 1139, 1417
HpyF3I CTNAG 5 cut(s) 291, 372, 431, 1153, 1210
HpySE526I ACGT 1 cut(s) 9
Hsp92I GRCGYC 1 cut(s) 1215
Hsp92II CATG 7 cut(s) 115, 547, 619, 770, 809, 1227, 1255
HspAI GCGC 2 cut(s) 489, 931
KspAI GTTAAC 1 cut(s) 1458
Kzo9I GATC 8 cut(s) 67, 247, 368, 483, 923, 939, 1284, 1378
LmnI GCTCC 5 cut(s) 26, 84, 121, 454, 1503
Lsp1109I GCAGC 5 cut(s) 114, 572, 646, 897, 901
LweI GCATC 3 cut(s) 897, 1087, 1398
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 5 cut(s) 126, 840, 868, 965, 1324
MalI GATC 8 cut(s) 69, 249, 370, 485, 925, 941, 1286, 1380
MboI GATC 8 cut(s) 67, 247, 368, 483, 923, 939, 1284, 1378
MboII GAAGA 9 cut(s) 21, 403, 454, 848, 898, 949, 1364, 1381, 1415
MflI RGATCY 2 cut(s) 368, 1378
MhlI GDGCHC 4 cut(s) 196, 432, 1178, 1441
MlsI TGGCCA 1 cut(s) 1250
MluCI AATT 3 cut(s) 262, 1365, 1385
MluNI TGGCCA 1 cut(s) 1250
MlyI GAGTC 3 cut(s) 388, 589, 1134
MmeI TCCRAC 2 cut(s) 326, 1383
Mox20I TGGCCA 1 cut(s) 1250
Mph1103I ATGCAT 1 cut(s) 613
MscI TGGCCA 1 cut(s) 1250
MseI TTAA 5 cut(s) 222, 1035, 1230, 1364, 1457
MslI CAYNNNNRTG 4 cut(s) 133, 548, 1268, 1434
Msp20I TGGCCA 1 cut(s) 1250
MspA1I CMGCKG 2 cut(s) 563, 914
MspI CCGG 4 cut(s) 82, 88, 1200, 1441
MspR9I CCNGG 2 cut(s) 1441, 1442
Mva1269I GAATGC 1 cut(s) 611
MvnI CGCG 1 cut(s) 816
MwoI GCNNNNNNNGC 9 cut(s) 93, 102, 130, 488, 625, 891, 1097, 1139, 1417
NciI CCSGG 2 cut(s) 1441, 1442
NcoI CCATGG 3 cut(s) 111, 805, 1251
NdeI CATATG 2 cut(s) 384, 607
NdeII GATC 8 cut(s) 67, 247, 368, 483, 923, 939, 1284, 1378
NlaIII CATG 7 cut(s) 115, 547, 619, 770, 809, 1227, 1255
NlaIV GGNNCC 4 cut(s) 86, 123, 1261, 1380
NmuCI GTSAC 3 cut(s) 126, 840, 868
NruI TCGCGA 1 cut(s) 816
NsiI ATGCAT 1 cut(s) 613
NspI RCATGY 1 cut(s) 770
OliI CACNNNNGTG 2 cut(s) 133, 1434
PceI AGGCCT 1 cut(s) 821
PciI ACATGT 1 cut(s) 766
PcsI WCGNNNNNNNCGW 1 cut(s) 136
PctI GAATGC 1 cut(s) 611
PfeI GAWTC 2 cut(s) 169, 1029
PflMI CCANNNNNTGG 1 cut(s) 112
PkrI GCNGC 7 cut(s) 104, 118, 306, 562, 661, 887, 916
Ple19I CGATCG 1 cut(s) 486
PleI GAGTC 3 cut(s) 387, 588, 1133
PpsI GAGTC 3 cut(s) 387, 588, 1133
PscI ACATGT 1 cut(s) 766
PshBI ATTAAT 1 cut(s) 1364
PspFI CCCAGC 1 cut(s) 491
PspN4I GGNNCC 4 cut(s) 86, 123, 1261, 1380
PspPI GGNCC 4 cut(s) 79, 648, 954, 1132
PstNI CAGNNNCTG 2 cut(s) 891, 1160
PsuI RGATCY 2 cut(s) 368, 1378
PvuI CGATCG 1 cut(s) 486
PvuII CAGCTG 2 cut(s) 563, 914
RruI TCGCGA 1 cut(s) 816
RsaI GTAC 2 cut(s) 254, 765
RsaNI GTAC 2 cut(s) 253, 764
RseI CAYNNNNRTG 4 cut(s) 133, 548, 1268, 1434
SaqAI TTAA 5 cut(s) 222, 1035, 1230, 1364, 1457
SatI GCNGC 7 cut(s) 103, 117, 305, 561, 660, 886, 915
Sau3AI GATC 8 cut(s) 67, 247, 368, 483, 923, 939, 1284, 1378
Sau96I GGNCC 4 cut(s) 79, 648, 954, 1132
SchI GAGTC 3 cut(s) 388, 589, 1134
ScrFI CCNGG 2 cut(s) 1441, 1442
SduI GDGCHC 4 cut(s) 196, 432, 1178, 1441
SfaNI GCATC 3 cut(s) 897, 1087, 1398
SinI GGWCC 2 cut(s) 648, 954
SmaI CCCGGG 1 cut(s) 1442
SmiMI CAYNNNNRTG 4 cut(s) 133, 548, 1268, 1434
SrfI GCCCGGGC 1 cut(s) 1442
Sse9I AATT 3 cut(s) 262, 1365, 1385
SseBI AGGCCT 1 cut(s) 821
SsiI CCGC 8 cut(s) 48, 60, 116, 119, 133, 200, 305, 398
StuI AGGCCT 1 cut(s) 821
StyD4I CCNGG 2 cut(s) 1439, 1440
StyI CCWWGG 4 cut(s) 111, 364, 805, 1251
TaaI ACNGT 3 cut(s) 337, 763, 899
TaiI ACGT 1 cut(s) 12
TaqI TCGA 4 cut(s) 922, 993, 1128, 1491
TaqII GACCGA 1 cut(s) 942
TasI AATT 3 cut(s) 262, 1365, 1385
TatI WGTACW 2 cut(s) 252, 763
TauI GCSGC 2 cut(s) 119, 307
TfiI GAWTC 2 cut(s) 169, 1029
Tru1I TTAA 5 cut(s) 222, 1035, 1230, 1364, 1457
Tru9I TTAA 5 cut(s) 222, 1035, 1230, 1364, 1457
TscAI CASTG 2 cut(s) 439, 1270
TseFI GTSAC 3 cut(s) 126, 840, 868
TseI GCWGC 5 cut(s) 102, 560, 659, 885, 914
Tsp45I GTSAC 3 cut(s) 126, 840, 868
TspDTI ATGAA 7 cut(s) 17, 161, 404, 604, 863, 995, 1358
TspGWI ACGGA 1 cut(s) 28
TspMI CCCGGG 1 cut(s) 1440
TspRI CASTG 2 cut(s) 439, 1270
Van91I CCANNNNNTGG 1 cut(s) 112
VpaK11BI GGWCC 2 cut(s) 648, 954
VspI ATTAAT 1 cut(s) 1364
XapI RAATTY 1 cut(s) 1385
XceI RCATGY 1 cut(s) 770
XmaI CCCGGG 1 cut(s) 1440
XmiI GTMKAC 3 cut(s) 583, 979, 1118
Zsp2I ATGCAT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.