Rmu_co8343623.1_g000001

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8343623.1
OG0007935
Physical Location & Seq
Forward (+)
49 .. 818
770 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8343623.1_g000001.1.cds

Sequence Viewer

Length: 525 bp
atgaacaacgtctctcagagctcctcctcttcctcctccgtcaactccgcctccgatccgaacgtgagccgcagccggaggagtgccgacggcagccaaaccatggcggctgagccgtcacggcggtggcgtgatgttttctggttggggatatttatgattcatttgattttattgggaggtgcgctggcggttctggggctcaataggtttaagagatcggataggctaaacttagataggttcaccagtaagtacttggaaaatcagaaaggattgaccgaggattattggccgctttatgctcttgctggtggagttgggactgttcttggttggacttggttgctgttccttggctcgcaggcgaatcacatgatgaagattgcggtgcatatcttgaccacttatcttgctgtgctcagtgttctgtgtttttggatggagcagttcttctggggagttgcatttgcgatcggcgctgggatgctcttcttgtatgttttggcagttatagacaggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

174

Amino Acids

19.46

Weight (kDa)

9.51

Isoelectric Point (pI)

53.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 103
AciI CCGC 7 cut(s) 48, 70, 107, 124, 191, 296, 389
AclWI GGATC 1 cut(s) 50
AcoI YGGCCR 1 cut(s) 293
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 1 cut(s) 103
AleI CACNNNNGTG 1 cut(s) 124
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw21I GWGCWC 2 cut(s) 23, 423
Alw26I GTCTC 1 cut(s) 16
AlwI GGATC 1 cut(s) 50
AoxI GGCC 1 cut(s) 293
ApeKI GCWGC 2 cut(s) 72, 93
AspLEI GCGC 2 cut(s) 187, 482
AsuHPI GGTGA 1 cut(s) 238
BanII GRGCYC 2 cut(s) 23, 204
Bbv12I GWGCWC 2 cut(s) 23, 423
BbvI GCAGC 2 cut(s) 84, 105
BccI CCATC 1 cut(s) 436
BceAI ACGGC 3 cut(s) 100, 106, 137
BcoDI GTCTC 1 cut(s) 16
BfoI RGCGCY 1 cut(s) 483
BglI GCCNNNNNGGC 1 cut(s) 121
BisI GCNGC 5 cut(s) 70, 73, 94, 108, 296
BlpI GCTNAGC 1 cut(s) 111
BlsI GCNGC 5 cut(s) 71, 74, 95, 109, 297
BmcAI AGTACT 1 cut(s) 257
BmsI GCATC 1 cut(s) 477
Bpu1102I GCTNAGC 1 cut(s) 111
BsaJI CCNNGG 3 cut(s) 102, 282, 355
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse1I ACTGG 1 cut(s) 249
BseDI CCNNGG 3 cut(s) 102, 282, 355
BseGI GGATG 2 cut(s) 447, 492
BseLI CCNNNNNNNGG 1 cut(s) 103
BseMII CTCAG 3 cut(s) 29, 102, 436
BseNI ACTGG 1 cut(s) 249
BseRI GAGGAG 4 cut(s) 13, 16, 25, 94
BseXI GCAGC 2 cut(s) 84, 105
BseYI CCCAGC 1 cut(s) 482
Bsh1285I CGRYCG 1 cut(s) 477
BshFI GGCC 1 cut(s) 295
BsiEI CGRYCG 1 cut(s) 477
BsiHKAI GWGCWC 2 cut(s) 23, 423
BsiSI CCGG 1 cut(s) 76
BslFI GGGAC 1 cut(s) 337
BslI CCNNNNNNNGG 1 cut(s) 103
BsmAI GTCTC 1 cut(s) 16
BsmBI CGTCTC 1 cut(s) 16
BsmFI GGGAC 1 cut(s) 337
BsnI GGCC 1 cut(s) 295
Bsp1286I GDGCHC 3 cut(s) 23, 204, 423
Bsp143I GATC 3 cut(s) 55, 218, 474
Bsp1720I GCTNAGC 1 cut(s) 111
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 7 cut(s) 48, 70, 107, 124, 191, 296, 389
BspANI GGCC 1 cut(s) 295
BspCNI CTCAG 3 cut(s) 28, 103, 435
BspPI GGATC 1 cut(s) 50
BspQI GCTCTTC 1 cut(s) 497
BsrI ACTGG 1 cut(s) 249
BssECI CCNNGG 3 cut(s) 102, 282, 355
BssMI GATC 3 cut(s) 55, 218, 474
BssT1I CCWWGG 2 cut(s) 102, 355
Bst4CI ACNGT 1 cut(s) 328
Bst6I CTCTTC 2 cut(s) 34, 497
BstC8I GCNNGC 3 cut(s) 189, 362, 366
BstDEI CTNAG 4 cut(s) 15, 111, 235, 422
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 2 cut(s) 447, 492
BstH2I RGCGCY 1 cut(s) 483
BstHHI GCGC 2 cut(s) 187, 482
BstKTI GATC 3 cut(s) 58, 221, 477
BstMAI GTCTC 1 cut(s) 16
BstMBI GATC 3 cut(s) 55, 218, 474
BstMCI CGRYCG 1 cut(s) 477
BstMWI GCNNNNNNNGC 2 cut(s) 121, 479
BstV1I GCAGC 2 cut(s) 84, 105
BsuRI GGCC 1 cut(s) 295
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 2 cut(s) 447, 492
BtsIMutI CAGTG 1 cut(s) 430
Cac8I GCNNGC 3 cut(s) 189, 362, 366
CfoI GCGC 2 cut(s) 187, 482
Csp6I GTAC 1 cut(s) 256
CspCI CAANNNNNGTGG 2 cut(s) 394, 429
CviAII CATG 2 cut(s) 103, 376
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 4 cut(s) 15, 111, 235, 422
DpnI GATC 3 cut(s) 57, 220, 476
DpnII GATC 3 cut(s) 55, 218, 474
EaeI YGGCCR 1 cut(s) 293
Eam1104I CTCTTC 2 cut(s) 34, 497
EarI CTCTTC 2 cut(s) 34, 497
EciI GGCGGA 1 cut(s) 37
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 2 cut(s) 102, 355
Eco24I GRGCYC 2 cut(s) 23, 204
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoT14I CCWWGG 2 cut(s) 102, 355
EcoT38I GRGCYC 2 cut(s) 23, 204
ErhI CCWWGG 2 cut(s) 102, 355
Esp3I CGTCTC 1 cut(s) 16
FaeI CATG 2 cut(s) 106, 379
FaiI YATR 7 cut(s) 104, 158, 303, 377, 396, 501, 515
FaqI GGGAC 1 cut(s) 337
FatI CATG 2 cut(s) 102, 375
Fnu4HI GCNGC 5 cut(s) 70, 73, 94, 108, 296
FokI GGATG 2 cut(s) 454, 499
FriOI GRGCYC 2 cut(s) 23, 204
Fsp4HI GCNGC 5 cut(s) 70, 73, 94, 108, 296
GlaI GCGC 2 cut(s) 186, 481
GluI GCNGC 5 cut(s) 70, 73, 94, 108, 296
GsaI CCCAGC 1 cut(s) 486
HaeII RGCGCY 1 cut(s) 483
HaeIII GGCC 1 cut(s) 295
HapII CCGG 1 cut(s) 76
HhaI GCGC 2 cut(s) 187, 482
Hin1II CATG 2 cut(s) 106, 379
Hin6I GCGC 2 cut(s) 185, 480
HinP1I GCGC 2 cut(s) 185, 480
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 2 cut(s) 160, 370
HpaII CCGG 1 cut(s) 76
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 2 cut(s) 43, 246
Hpy188I TCNGA 5 cut(s) 18, 55, 60, 223, 270
Hpy188III TCNNGA 1 cut(s) 400
Hpy8I GTNNAC 2 cut(s) 43, 246
Hpy99I CGWCG 1 cut(s) 92
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4IV ACGT 2 cut(s) 9, 63
HpyCH4V TGCA 2 cut(s) 394, 467
HpyF10VI GCNNNNNNNGC 2 cut(s) 121, 479
HpyF3I CTNAG 4 cut(s) 15, 111, 235, 422
HpySE526I ACGT 2 cut(s) 9, 63
Hsp92II CATG 2 cut(s) 106, 379
HspAI GCGC 2 cut(s) 185, 480
Kzo9I GATC 3 cut(s) 55, 218, 474
LguI GCTCTTC 1 cut(s) 497
LmnI GCTCC 2 cut(s) 26, 445
Lsp1109I GCAGC 2 cut(s) 84, 105
LweI GCATC 1 cut(s) 477
MaeII ACGT 2 cut(s) 9, 63
MaeIII GTNAC 1 cut(s) 117
MalI GATC 3 cut(s) 57, 220, 476
MboI GATC 3 cut(s) 55, 218, 474
MboII GAAGA 4 cut(s) 21, 394, 445, 484
MhlI GDGCHC 3 cut(s) 23, 204, 423
MmeI TCCRAC 1 cut(s) 317
MnlI CCTC 8 cut(s) 34, 37, 43, 46, 61, 72, 173, 277
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 1 cut(s) 124
MspI CCGG 1 cut(s) 76
MwoI GCNNNNNNNGC 2 cut(s) 121, 479
NcoI CCATGG 1 cut(s) 102
NdeII GATC 3 cut(s) 55, 218, 474
NlaIII CATG 2 cut(s) 106, 379
NmuCI GTSAC 1 cut(s) 117
OliI CACNNNNGTG 1 cut(s) 124
PciSI GCTCTTC 1 cut(s) 497
PcsI WCGNNNNNNNCGW 1 cut(s) 127
PfeI GAWTC 2 cut(s) 160, 370
PflMI CCANNNNNTGG 1 cut(s) 103
PkrI GCNGC 5 cut(s) 71, 74, 95, 109, 297
Ple19I CGATCG 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 23
PspFI CCCAGC 1 cut(s) 482
PvuI CGATCG 1 cut(s) 477
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
RseI CAYNNNNRTG 1 cut(s) 124
SacI GAGCTC 1 cut(s) 23
SapI GCTCTTC 1 cut(s) 497
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 5 cut(s) 70, 73, 94, 108, 296
Sau3AI GATC 3 cut(s) 55, 218, 474
ScaI AGTACT 1 cut(s) 257
SduI GDGCHC 3 cut(s) 23, 204, 423
SetI ASST 7 cut(s) 12, 23, 66, 184, 212, 245, 524
SfaNI GCATC 1 cut(s) 477
SmiMI CAYNNNNRTG 1 cut(s) 124
SsiI CCGC 7 cut(s) 48, 70, 107, 124, 191, 296, 389
SstI GAGCTC 1 cut(s) 23
StyI CCWWGG 2 cut(s) 102, 355
TaaI ACNGT 1 cut(s) 328
TaiI ACGT 2 cut(s) 12, 66
TaqII GACCGA 1 cut(s) 296
TatI WGTACW 1 cut(s) 255
TauI GCSGC 3 cut(s) 72, 110, 298
TfiI GAWTC 2 cut(s) 160, 370
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 1 cut(s) 430
TseFI GTSAC 1 cut(s) 117
TseI GCWGC 2 cut(s) 72, 93
Tsp45I GTSAC 1 cut(s) 117
TspDTI ATGAA 3 cut(s) 17, 152, 395
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 1 cut(s) 430
Van91I CCANNNNNTGG 1 cut(s) 103
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
ZrmI AGTACT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.