MD13G1044800.v1.1

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007935
Physical Location & Seq
Reverse (-)
3107349 .. 3110663
3315 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1044800.v1.1.491

Sequence Viewer

Length: 1509 bp
ATGAACAACATCTGTCACAGCTCCTCCGCCTCCTCCACCTCCACCTCCTCCTCCATCAACACCGCTGATCCGAATGTAGTCTCGAGTCGGAGGAGCGTGGATGGTGGTCAAGCCCTGGCAGCTGAGCCATCGCGTCGATGGCGTGACATTTTCTGGTTGGGAGTGTTTCTGATTCATTTGATTTTGGTGGGATGTGCTCTTACGGTTCTGGGGACCAATAGGTTTAAGAAAACAGATAGGCTAAACATAGATAGGTACACCCAGAGATTTTTGGAAAATGAAAGGGGGTTGACGGAGAACTATTGGCTGCTTTATGCTCTTGCCGGGGGAGTTGGGACACTTCTTGGTTGGACATGGTTGTTTTTCCTCCGTTCTCAAGCGACTCACATGATGAAGGTTGCGGTTCACATCTTGACTACTTATCTTGCTGTGATTAGTGTTCTATTGTTCTGGATGGAACAATTTTTCTGGGGTGTTGCATTTGCGATTGGTGCTGTTTTGCAGTTCATGTATGTTCTTTCAATTGTGGACAGACTTCCCTTTACCATGCTGGTGCTGCAAGCAGCTGTCAAGATGGTATGGAGTCTTCGTGAAGTTATGAGAGTAGCATATGCTTTCATGCTTATTATGCTTTTATGGATGGTATTATGGTCCTTCGGGGCAGCTGGTGTTGTAGCGTCAAGCATTGGAGATGGTGGACGCTGGTGGCTTCTTGTGGTTCTCTCTATAAGCTTATTTTGGACGGGTGCAGTACTCTGTAATACTGTGCATGTTATAGTGTCTGGGATGATGTTTCTTGTTCTTATCCATGGTGGTAGGGAGGCACCATCAATGCCTCCAAACTCACTGATGAAGTCTCTTCGGTATGCAGTCACAACTTCTTTTGGCAGTATCTGCTATGGTTCACTTTTCACAGCTTTCATTCGGACACTGCGGTGGAAGATCAGGGGAGTCCGGTCCAAGATAGGCAACAACGAGTGTCTACTTTGCTGTGTTGATTTTCTCTTTCATCTAGTGGAGACTCTTGTCCGTTTCTTTAACAAGTATGCCTATGTGCAGATAGCTGTTTCTGGCAAAGGATTCAATCATTCAGCGAGGGATGCCTGGGAGTTATTTCAGTCTACTGGAGTGGAGGCTCTTGTTGCCTACGATTGTTCAGGTGCGGTTTTATTGATGGGAACAGTTTTGGGCGGGCTTATTACTGGAACTTCCGCCGGGATCTGGACATGGATTAAGTACAGGGACAGAGTGGTTATGGTGGGGTCCACTGCAATGTTGATGGGAATGGTCTTGGTGGGGCTGGCGATGGTGGTTGTGGAAAGTGCCGTTACATCTATTTATATTTGTTATGCAGAAGACCCCTTGTTGATTCGCAGGTGGGATGCTGAGTTCTTCAACCAGATGTCGGAGATGCTACACCAGCGTCTTCAACATAGGAGCTCACGAGCGAGGGAAGTGTTGACCCACCATCACCTTGATAACCACACACAAGAAACACTCCCCGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

503

Amino Acids

56.35

Weight (kDa)

9.21

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 160 - 454 3.5e-58 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1365
Acc36I ACCTGC 1 cut(s) 1365
AccB1I GGYRCC 1 cut(s) 823
AccI GTMKAC 2 cut(s) 982, 1121
AccII CGCG 1 cut(s) 133
AciI CCGC 8 cut(s) 27, 63, 401, 934, 1163, 1191, 1212, 1503
AclWI GGATC 2 cut(s) 62, 1226
AfaI GTAC 3 cut(s) 257, 753, 1238
AfiI CCNNNNNNNGG 2 cut(s) 1221, 1405
AgsI TTSAA 4 cut(s) 522, 1084, 1396, 1430
AjnI CCWGG 2 cut(s) 114, 1103
AleI CACNNNNGTG 1 cut(s) 934
AloI GAACNNNNNNTCC 2 cut(s) 1373, 1405
AluBI AGCT 8 cut(s) 21, 122, 566, 665, 732, 917, 1064, 1440
AluI AGCT 8 cut(s) 21, 122, 566, 665, 732, 917, 1064, 1440
Alw21I GWGCWC 2 cut(s) 199, 1442
Alw26I GTCTC 3 cut(s) 85, 861, 1013
AlwI GGATC 2 cut(s) 62, 1226
AlwNI CAGNNNCTG 1 cut(s) 894
Ama87I CYCGRG 1 cut(s) 82
ApeKI GCWGC 5 cut(s) 119, 307, 556, 563, 662
AspS9I GGNCC 4 cut(s) 213, 651, 957, 1263
AsuC2I CCSGG 2 cut(s) 325, 1216
AsuHPI GGTGA 1 cut(s) 1463
AvaI CYCGRG 1 cut(s) 82
AvaII GGWCC 4 cut(s) 213, 651, 957, 1263
BanI GGYRCC 1 cut(s) 823
BanII GRGCYC 1 cut(s) 1442
BarI GAAGNNNNNNTAC 2 cut(s) 570, 602
BauI CACGAG 1 cut(s) 1443
BbsI GAAGAC 3 cut(s) 578, 1362, 1418
Bbv12I GWGCWC 2 cut(s) 199, 1442
BbvI GCAGC 5 cut(s) 131, 294, 543, 575, 674
BceAI ACGGC 1 cut(s) 1310
BciT130I CCWGG 2 cut(s) 116, 1105
BcnI CCSGG 2 cut(s) 325, 1216
BcoDI GTCTC 3 cut(s) 85, 861, 1013
BfaI CTAG 1 cut(s) 1013
BfuAI ACCTGC 1 cut(s) 1365
BisI GCNGC 5 cut(s) 120, 308, 557, 564, 663
BlpI GCTNAGC 1 cut(s) 123
BlsI GCNGC 5 cut(s) 121, 309, 558, 565, 664
BmcAI AGTACT 1 cut(s) 753
Bme1390I CCNGG 4 cut(s) 116, 325, 1105, 1216
Bme18I GGWCC 4 cut(s) 213, 651, 957, 1263
BmeT110I CYCGRG 1 cut(s) 82
BmgT120I GGNCC 4 cut(s) 213, 651, 957, 1263
BmiI GGNNCC 3 cut(s) 214, 825, 1264
BmrFI CCNGG 4 cut(s) 116, 325, 1105, 1216
BmsI GCATC 3 cut(s) 1090, 1372, 1401
BoxI GACNNNNGTC 1 cut(s) 1025
BpiI GAAGAC 3 cut(s) 578, 1362, 1418
BpmI CTGGAG 1 cut(s) 1146
Bpu1102I GCTNAGC 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 360
BpuMI CCSGG 2 cut(s) 325, 1216
BsaJI CCNNGG 4 cut(s) 114, 324, 808, 1104
BsaWI WCCGGW 1 cut(s) 954
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 574, 604
Bsc4I CCNNNNNNNGG 2 cut(s) 1221, 1405
Bse1I ACTGG 2 cut(s) 1129, 1207
Bse3DI GCAATG 1 cut(s) 1278
BseBI CCWGG 2 cut(s) 116, 1105
BseDI CCNNGG 4 cut(s) 114, 324, 808, 1104
BseGI GGATG 7 cut(s) 106, 197, 459, 645, 792, 1105, 1387
BseLI CCNNNNNNNGG 2 cut(s) 1221, 1405
BseMI GCAATG 1 cut(s) 1278
BseMII CTCAG 2 cut(s) 114, 1377
BseNI ACTGG 2 cut(s) 1129, 1207
BseRI GAGGAG 5 cut(s) 13, 22, 37, 40, 106
BseXI GCAGC 5 cut(s) 131, 294, 543, 575, 674
BsgI GTGCAG 2 cut(s) 768, 1076
Bsh1236I CGCG 1 cut(s) 133
BshNI GGYRCC 1 cut(s) 823
BsiHKAI GWGCWC 2 cut(s) 199, 1442
BsiHKCI CYCGRG 1 cut(s) 82
BsiSI CCGG 3 cut(s) 324, 955, 1215
BslFI GGGAC 3 cut(s) 226, 349, 1256
BslI CCNNNNNNNGG 2 cut(s) 1221, 1405
BsmAI GTCTC 3 cut(s) 85, 861, 1013
BsmFI GGGAC 3 cut(s) 226, 349, 1256
BsoBI CYCGRG 1 cut(s) 82
Bsp1286I GDGCHC 2 cut(s) 199, 1442
Bsp143I GATC 3 cut(s) 67, 942, 1218
Bsp1720I GCTNAGC 1 cut(s) 123
Bsp19I CCATGG 1 cut(s) 808
BspACI CCGC 8 cut(s) 27, 63, 401, 934, 1163, 1191, 1212, 1503
BspCNI CTCAG 2 cut(s) 115, 1378
BspFNI CGCG 1 cut(s) 133
BspLI GGNNCC 3 cut(s) 214, 825, 1264
BspMI ACCTGC 1 cut(s) 1365
BspPI GGATC 2 cut(s) 62, 1226
BspT107I GGYRCC 1 cut(s) 823
BsrDI GCAATG 1 cut(s) 1278
BsrI ACTGG 2 cut(s) 1129, 1207
BssECI CCNNGG 4 cut(s) 114, 324, 808, 1104
BssMI GATC 3 cut(s) 67, 942, 1218
BssSI CACGAG 1 cut(s) 1443
BssT1I CCWWGG 1 cut(s) 808
Bst2BI CACGAG 1 cut(s) 1443
Bst2UI CCWGG 2 cut(s) 116, 1105
Bst4CI ACNGT 3 cut(s) 205, 766, 1183
Bst6I CTCTTC 1 cut(s) 864
BstAPI GCANNNNNTGC 1 cut(s) 894
BstC8I GCNNGC 3 cut(s) 561, 1193, 1302
BstDEI CTNAG 2 cut(s) 123, 1386
BstDSI CCRYGG 1 cut(s) 808
BstF5I GGATG 7 cut(s) 106, 197, 459, 645, 792, 1105, 1387
BstFNI CGCG 1 cut(s) 133
BstKTI GATC 3 cut(s) 70, 945, 1221
BstMAI GTCTC 3 cut(s) 85, 861, 1013
BstMBI GATC 3 cut(s) 67, 942, 1218
BstMWI GCNNNNNNNGC 9 cut(s) 119, 139, 491, 556, 628, 894, 1100, 1142, 1420
BstNI CCWGG 2 cut(s) 116, 1105
BstNSI RCATGY 1 cut(s) 773
BstPAI GACNNNNGTC 1 cut(s) 1025
BstSCI CCNGG 4 cut(s) 114, 323, 1103, 1214
BstUI CGCG 1 cut(s) 133
BstV1I GCAGC 5 cut(s) 131, 294, 543, 575, 674
BstV2I GAAGAC 3 cut(s) 578, 1362, 1418
BstX2I RGATCY 1 cut(s) 1218
BstYI RGATCY 1 cut(s) 1218
BtgI CCRYGG 1 cut(s) 808
BtgZI GCGATG 2 cut(s) 114, 1319
BtsCI GGATG 7 cut(s) 106, 197, 459, 645, 792, 1105, 1387
BtsI GCAGTG 2 cut(s) 929, 1266
BtsIMutI CAGTG 3 cut(s) 845, 929, 1266
BveI ACCTGC 1 cut(s) 1365
Cac8I GCNNGC 3 cut(s) 561, 1193, 1302
CaiI CAGNNNCTG 1 cut(s) 894
Cfr13I GGNCC 4 cut(s) 213, 651, 957, 1263
CseI GACGC 4 cut(s) 122, 666, 708, 1412
Csp6I GTAC 3 cut(s) 256, 752, 1237
CviAII CATG 8 cut(s) 354, 388, 508, 547, 619, 770, 809, 1227
CviQI GTAC 3 cut(s) 256, 752, 1237
DdeI CTNAG 2 cut(s) 123, 1386
DpnI GATC 3 cut(s) 69, 944, 1220
DpnII GATC 3 cut(s) 67, 942, 1218
Eam1104I CTCTTC 1 cut(s) 864
EarI CTCTTC 1 cut(s) 864
EciI GGCGGA 2 cut(s) 16, 1201
Ecl136II GAGCTC 1 cut(s) 1440
Eco130I CCWWGG 1 cut(s) 808
Eco24I GRGCYC 1 cut(s) 1442
Eco47I GGWCC 4 cut(s) 213, 651, 957, 1263
Eco53kI GAGCTC 1 cut(s) 1440
Eco88I CYCGRG 1 cut(s) 82
EcoICRI GAGCTC 1 cut(s) 1440
EcoRII CCWGG 2 cut(s) 114, 1103
EcoT14I CCWWGG 1 cut(s) 808
EcoT38I GRGCYC 1 cut(s) 1442
ErhI CCWWGG 1 cut(s) 808
FaeI CATG 8 cut(s) 357, 391, 511, 550, 622, 773, 812, 1230
FaqI GGGAC 3 cut(s) 226, 349, 1256
FatI CATG 8 cut(s) 353, 387, 507, 546, 618, 769, 808, 1226
FauI CCCGC 1 cut(s) 1184
FauNDI CATATG 1 cut(s) 610
FblI GTMKAC 2 cut(s) 982, 1121
Fnu4HI GCNGC 5 cut(s) 120, 308, 557, 564, 663
FokI GGATG 7 cut(s) 113, 204, 466, 652, 799, 1112, 1394
FriOI GRGCYC 1 cut(s) 1442
Fsp4HI GCNGC 5 cut(s) 120, 308, 557, 564, 663
FspBI CTAG 1 cut(s) 1013
GluI GCNGC 5 cut(s) 120, 308, 557, 564, 663
GsuI CTGGAG 1 cut(s) 1146
HapII CCGG 3 cut(s) 324, 955, 1215
HgaI GACGC 4 cut(s) 122, 666, 708, 1412
Hin1II CATG 8 cut(s) 357, 391, 511, 550, 622, 773, 812, 1230
HincII GTYRAC 2 cut(s) 291, 1461
HindII GTYRAC 2 cut(s) 291, 1461
HindIII AAGCTT 1 cut(s) 730
HinfI GANTC 8 cut(s) 85, 172, 382, 583, 951, 1021, 1080, 1369
HpaII CCGG 3 cut(s) 324, 955, 1215
HphI GGTGA 1 cut(s) 1463
Hpy188I TCNGA 5 cut(s) 72, 90, 171, 927, 1408
Hpy188III TCNNGA 7 cut(s) 82, 412, 451, 571, 590, 1222, 1443
Hpy99I CGWCG 1 cut(s) 138
HpyAV CCTTC 2 cut(s) 388, 664
HpyCH4III ACNGT 3 cut(s) 205, 766, 1183
HpyCH4V TGCA 9 cut(s) 479, 502, 559, 749, 769, 869, 1057, 1271, 1352
HpyF10VI GCNNNNNNNGC 9 cut(s) 119, 139, 491, 556, 628, 894, 1100, 1142, 1420
HpyF3I CTNAG 2 cut(s) 123, 1386
Hsp92II CATG 8 cut(s) 357, 391, 511, 550, 622, 773, 812, 1230
Kzo9I GATC 3 cut(s) 67, 942, 1218
LmnI GCTCC 3 cut(s) 26, 93, 1437
Lsp1109I GCAGC 5 cut(s) 131, 294, 543, 575, 674
LweI GCATC 3 cut(s) 1090, 1372, 1401
MaeI CTAG 1 cut(s) 1013
MaeIII GTNAC 4 cut(s) 14, 143, 871, 1327
MalI GATC 3 cut(s) 69, 944, 1220
MboI GATC 3 cut(s) 67, 942, 1218
MboII GAAGA 6 cut(s) 578, 851, 952, 1367, 1384, 1418
MfeI CAATTG 1 cut(s) 522
MflI RGATCY 1 cut(s) 1218
MhlI GDGCHC 2 cut(s) 199, 1442
MluCI AATT 2 cut(s) 461, 522
MlyI GAGTC 5 cut(s) 94, 376, 592, 960, 1015
MmeI TCCRAC 3 cut(s) 68, 329, 1386
MseI TTAA 3 cut(s) 225, 1038, 1233
MslI CAYNNNNRTG 3 cut(s) 551, 934, 1271
MspA1I CMGCKG 4 cut(s) 65, 122, 566, 665
MspI CCGG 3 cut(s) 324, 955, 1215
MspR9I CCNGG 4 cut(s) 116, 325, 1105, 1216
MunI CAATTG 1 cut(s) 522
MvaI CCWGG 2 cut(s) 116, 1105
MvnI CGCG 1 cut(s) 133
MwoI GCNNNNNNNGC 9 cut(s) 119, 139, 491, 556, 628, 894, 1100, 1142, 1420
NciI CCSGG 2 cut(s) 325, 1216
NcoI CCATGG 1 cut(s) 808
NdeI CATATG 1 cut(s) 610
NdeII GATC 3 cut(s) 67, 942, 1218
NlaIII CATG 8 cut(s) 357, 391, 511, 550, 622, 773, 812, 1230
NlaIV GGNNCC 3 cut(s) 214, 825, 1264
NmuCI GTSAC 3 cut(s) 14, 143, 871
NspI RCATGY 1 cut(s) 773
OliI CACNNNNGTG 1 cut(s) 934
PaeR7I CTCGAG 1 cut(s) 82
PaqCI CACCTGC 1 cut(s) 1365
PfeI GAWTC 3 cut(s) 172, 1080, 1369
PkrI GCNGC 5 cut(s) 121, 309, 558, 565, 664
PleI GAGTC 5 cut(s) 93, 376, 591, 959, 1015
PpsI GAGTC 5 cut(s) 93, 376, 591, 959, 1015
PshAI GACNNNNGTC 1 cut(s) 1025
Psp124BI GAGCTC 1 cut(s) 1442
Psp6I CCWGG 2 cut(s) 114, 1103
PspGI CCWGG 2 cut(s) 114, 1103
PspN4I GGNNCC 3 cut(s) 214, 825, 1264
PspPI GGNCC 4 cut(s) 213, 651, 957, 1263
PstNI CAGNNNCTG 1 cut(s) 894
PsuI RGATCY 1 cut(s) 1218
PvuII CAGCTG 3 cut(s) 122, 566, 665
RsaI GTAC 3 cut(s) 257, 753, 1238
RsaNI GTAC 3 cut(s) 256, 752, 1237
RseI CAYNNNNRTG 3 cut(s) 551, 934, 1271
SacI GAGCTC 1 cut(s) 1442
SaqAI TTAA 3 cut(s) 225, 1038, 1233
SatI GCNGC 5 cut(s) 120, 308, 557, 564, 663
Sau3AI GATC 3 cut(s) 67, 942, 1218
Sau96I GGNCC 4 cut(s) 213, 651, 957, 1263
ScaI AGTACT 1 cut(s) 753
SchI GAGTC 5 cut(s) 94, 376, 592, 960, 1015
ScrFI CCNGG 4 cut(s) 116, 325, 1105, 1216
SduI GDGCHC 2 cut(s) 199, 1442
SfaNI GCATC 3 cut(s) 1090, 1372, 1401
Sfr274I CTCGAG 1 cut(s) 82
SinI GGWCC 4 cut(s) 213, 651, 957, 1263
SlaI CTCGAG 1 cut(s) 82
SmiMI CAYNNNNRTG 3 cut(s) 551, 934, 1271
SmlI CTYRAG 2 cut(s) 82, 375
SmoI CTYRAG 2 cut(s) 82, 375
Sse9I AATT 2 cut(s) 461, 522
SsiI CCGC 8 cut(s) 27, 63, 401, 934, 1163, 1191, 1212, 1503
SspMI CTAG 1 cut(s) 1013
SstI GAGCTC 1 cut(s) 1442
StyD4I CCNGG 4 cut(s) 114, 323, 1103, 1214
StyI CCWWGG 1 cut(s) 808
TaaI ACNGT 3 cut(s) 205, 766, 1183
TaqI TCGA 2 cut(s) 83, 136
TasI AATT 2 cut(s) 461, 522
TatI WGTACW 2 cut(s) 751, 1236
TfiI GAWTC 3 cut(s) 172, 1080, 1369
Tru1I TTAA 3 cut(s) 225, 1038, 1233
Tru9I TTAA 3 cut(s) 225, 1038, 1233
TscAI CASTG 3 cut(s) 852, 936, 1273
TseFI GTSAC 3 cut(s) 14, 143, 871
TseI GCWGC 5 cut(s) 119, 307, 556, 563, 662
Tsp45I GTSAC 3 cut(s) 14, 143, 871
TspDTI ATGAA 9 cut(s) 17, 164, 294, 407, 496, 607, 866, 910, 998
TspGWI ACGGA 3 cut(s) 308, 359, 1019
TspRI CASTG 3 cut(s) 852, 936, 1273
VpaK11BI GGWCC 4 cut(s) 213, 651, 957, 1263
XceI RCATGY 1 cut(s) 773
XcmI CCANNNNNNNNNTGG 1 cut(s) 135
XhoI CTCGAG 1 cut(s) 82
XmiI GTMKAC 2 cut(s) 982, 1121
XspI CTAG 1 cut(s) 1013
ZrmI AGTACT 1 cut(s) 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.