Rh4CG365500

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0007935
Physical Location & Seq
Reverse (-)
62100154 .. 62103569
3416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG365500.1

Sequence Viewer

Length: 1398 bp
ATGGCGGCTGAGCCGTCACGGCGGTGGCGTGATGTTTTCTGGTTGGGGATATTTATGATTCATTTGATTTTATTGGGAGGTGCGCTGGCGGTTCTGGGGCTCAATAGGTTTAAGAGATCGGATAGGCTAAACTTAGATAGGTTCACCAATAAGTACTTGGAAAATCAGAAGGGATTGACTGAGGATTATTGGCCGCTTTATGCTCTTGCTGGTGGAGTTGGGACTGTTCTTGGTTGGACTTGGTTGCTGTTCCTTGGCTCGCAGGCGAATCACATGATGAAGATTGCGGTGCATATCTTGACCACTTATCTTGCTGTGCTCAGTGTTCTGTGTTTTTGGATGGAGCAGTTCTTCTGGGGAGTTGCATTTGCGATCGGCGCTGGGATGCTCTTCTTGTATGTTTTGGCAGTTATAGACAGACTACCCTTTACCATGCTCGTGCTACAAGCAGCTGTCAAGATGGTATGGAGTCTACCTGAAGTTATGAGGGTAGCATATGCATTCATGCTTATTATGCTTTTATGGATGGGAATATGGTCCTTTGGGGCTGCTGGTGTTGTAGCTTTAAGCATTGGAGATGGTGGACGCTGGTGGCTTCTTGTGGTTCTCTCTGTAAGCTTATTTTGGACGGGTGCTGTTCTCTGTAATACTGTACATGTTATAGTGTCTGGGATGGTGTTTCTTGTTCTTTCCCATGGTGGTCGAGAGGCCTCATCAATGCCTCCGAAGTCACTAATGAAGTCTCTTCGGTATGCAGTCACAACTTCTTTTGGCAGCATCTGCTATGGTTCACTTTTCACAGCTGCCATTCGAACTTTGCGCTGGAAGATCAGGGGAGTTCGGTCCAAGATTGGCAACAATGAGTGTCTACTTTGCTGTGTTGATTTCCTCTTTCATCTTGTGGAGACACTTGTGCGATTCTTTAATAAGTATGCTTACGTCCAGATAGCTGTTTCTGGCAAAGGTTTCAATCATTCAGCGAGGGATGCTTGGGAGTTATTTCAGTCTACTGGAGTCGAGGCACTCATTGCCTATGATTGCTCAGGTGCTGTATTATTGATGGGCACACTGTTGGGTGGGCTTATTACCGGAACTTGCTCGGGGATCTGGACATGGATTAAATATAGGGACAGAGTGGCTATGGTCGGGTCCACTGCAATGTTGATGGGAATGATCTTGGTGGGATTAGCAATGGTGGTTGTGGAAAGCGCTGTCACATCTATCTACATCTGTTATGCAGAAGACCCGTTGTTGATTCATAGGTGGGATGCTGAATTCTTCAACCAGATGTCGGAGATGCTACACCAGCGTCTTCAACATAGGAGTGCCCGGGCAAGGGAAGTGTTGACCCACAATCGGGTTGATAACCACCACACAGTCGAAACACTCCCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

465

Amino Acids

52.22

Weight (kDa)

9.11

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 122 - 416 2.3e-58 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 472, 868, 1007
AciI CCGC 5 cut(s) 5, 22, 89, 194, 287
AclWI GGATC 1 cut(s) 1112
AcoI YGGCCR 1 cut(s) 191
AcsI RAATTY 1 cut(s) 1274
AcuI CTGAAG 1 cut(s) 498
AfaI GTAC 2 cut(s) 155, 654
AfeI AGCGCT 1 cut(s) 1210
AfiI CCNNNNNNNGG 5 cut(s) 1291, 1335, 1336, 1356, 1357
AflIII ACRYGT 1 cut(s) 655
AgsI TTSAA 3 cut(s) 970, 1282, 1316
AleI CACNNNNGTG 1 cut(s) 22
AluBI AGCT 5 cut(s) 452, 563, 618, 803, 950
AluI AGCT 5 cut(s) 452, 563, 618, 803, 950
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 2 cut(s) 747, 899
AlwI GGATC 1 cut(s) 1112
AlwNI CAGNNNCTG 2 cut(s) 780, 1049
Ama87I CYCGRG 2 cut(s) 1099, 1329
Aor51HI AGCGCT 1 cut(s) 1210
AoxI GGCC 2 cut(s) 191, 708
ApeKI GCWGC 4 cut(s) 449, 548, 774, 803
ApoI RAATTY 1 cut(s) 1274
AspLEI GCGC 4 cut(s) 85, 380, 822, 1211
AspS9I GGNCC 3 cut(s) 537, 843, 1149
AsuC2I CCSGG 2 cut(s) 1330, 1331
AsuHPI GGTGA 1 cut(s) 136
AsuII TTCGAA 1 cut(s) 811
AvaI CYCGRG 2 cut(s) 1099, 1329
AvaII GGWCC 3 cut(s) 537, 843, 1149
BaeGI GKGCMC 2 cut(s) 1067, 1330
BanII GRGCYC 1 cut(s) 102
BauI CACGAG 1 cut(s) 437
BbsI GAAGAC 2 cut(s) 1248, 1304
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 4 cut(s) 461, 535, 786, 790
BccI CCATC 7 cut(s) 334, 454, 520, 572, 667, 1054, 1159
BceAI ACGGC 1 cut(s) 35
BcnI CCSGG 2 cut(s) 1330, 1331
BcoDI GTCTC 2 cut(s) 747, 899
BfoI RGCGCY 2 cut(s) 381, 1212
BglI GCCNNNNNGGC 1 cut(s) 19
BisI GCNGC 6 cut(s) 6, 194, 450, 549, 775, 804
BlpI GCTNAGC 1 cut(s) 9
BlsI GCNGC 6 cut(s) 7, 195, 451, 550, 776, 805
BmcAI AGTACT 1 cut(s) 155
Bme1390I CCNGG 2 cut(s) 1330, 1331
Bme18I GGWCC 3 cut(s) 537, 843, 1149
BmeT110I CYCGRG 2 cut(s) 1099, 1329
BmgT120I GGNCC 3 cut(s) 537, 843, 1149
BmiI GGNNCC 1 cut(s) 1150
BmrFI CCNGG 2 cut(s) 1330, 1331
BmsI GCATC 5 cut(s) 375, 786, 976, 1258, 1287
BpiI GAAGAC 2 cut(s) 1248, 1304
BpmI CTGGAG 1 cut(s) 1032
Bpu10I CCTNAGC 1 cut(s) 1042
Bpu1102I GCTNAGC 1 cut(s) 9
Bpu14I TTCGAA 1 cut(s) 811
BpuMI CCSGG 2 cut(s) 1330, 1331
BsaJI CCNNGG 3 cut(s) 253, 694, 1329
BsaWI WCCGGW 1 cut(s) 1088
Bsc4I CCNNNNNNNGG 5 cut(s) 1291, 1335, 1336, 1356, 1357
Bse1I ACTGG 1 cut(s) 1015
Bse3DI GCAATG 3 cut(s) 1026, 1164, 1197
BseDI CCNNGG 3 cut(s) 253, 694, 1329
BseGI GGATG 6 cut(s) 345, 390, 531, 678, 991, 1273
BseLI CCNNNNNNNGG 5 cut(s) 1291, 1335, 1336, 1356, 1357
BseMI GCAATG 3 cut(s) 1026, 1164, 1197
BseMII CTCAG 3 cut(s) 171, 334, 1056
BseNI ACTGG 1 cut(s) 1015
BseSI GKGCMC 2 cut(s) 1067, 1330
BseXI GCAGC 4 cut(s) 461, 535, 786, 790
BseYI CCCAGC 1 cut(s) 380
Bsh1285I CGRYCG 1 cut(s) 375
BshFI GGCC 2 cut(s) 193, 710
BsiEI CGRYCG 1 cut(s) 375
BsiHKAI GWGCWC 1 cut(s) 321
BsiHKCI CYCGRG 2 cut(s) 1099, 1329
BsiSI CCGG 2 cut(s) 1089, 1330
BslFI GGGAC 2 cut(s) 235, 1142
BslI CCNNNNNNNGG 5 cut(s) 1291, 1335, 1336, 1356, 1357
BsmAI GTCTC 2 cut(s) 747, 899
BsmFI GGGAC 2 cut(s) 235, 1142
BsmI GAATGC 1 cut(s) 500
BsnI GGCC 2 cut(s) 193, 710
BsoBI CYCGRG 2 cut(s) 1099, 1329
Bsp119I TTCGAA 1 cut(s) 811
Bsp1286I GDGCHC 4 cut(s) 102, 321, 1067, 1330
Bsp1407I TGTACA 1 cut(s) 652
Bsp143I GATC 5 cut(s) 116, 372, 828, 1104, 1173
Bsp1720I GCTNAGC 1 cut(s) 9
Bsp19I CCATGG 1 cut(s) 694
BspACI CCGC 5 cut(s) 5, 22, 89, 194, 287
BspANI GGCC 2 cut(s) 193, 710
BspCNI CTCAG 3 cut(s) 172, 333, 1055
BspLI GGNNCC 1 cut(s) 1150
BspPI GGATC 1 cut(s) 1112
BspQI GCTCTTC 1 cut(s) 395
BspT104I TTCGAA 1 cut(s) 811
BsrDI GCAATG 3 cut(s) 1026, 1164, 1197
BsrGI TGTACA 1 cut(s) 652
BsrI ACTGG 1 cut(s) 1015
BssECI CCNNGG 3 cut(s) 253, 694, 1329
BssMI GATC 5 cut(s) 116, 372, 828, 1104, 1173
BssSI CACGAG 1 cut(s) 437
BssT1I CCWWGG 2 cut(s) 253, 694
Bst2BI CACGAG 1 cut(s) 437
Bst4CI ACNGT 4 cut(s) 226, 652, 1071, 1378
Bst6I CTCTTC 2 cut(s) 395, 750
BstAPI GCANNNNNTGC 2 cut(s) 780, 1028
BstAUI TGTACA 1 cut(s) 652
BstBI TTCGAA 1 cut(s) 811
BstC8I GCNNGC 3 cut(s) 87, 260, 264
BstDEI CTNAG 5 cut(s) 9, 133, 180, 320, 1042
BstDSI CCRYGG 1 cut(s) 694
BstF5I GGATG 6 cut(s) 345, 390, 531, 678, 991, 1273
BstH2I RGCGCY 2 cut(s) 381, 1212
BstHHI GCGC 4 cut(s) 85, 380, 822, 1211
BstKTI GATC 5 cut(s) 119, 375, 831, 1107, 1176
BstMAI GTCTC 2 cut(s) 747, 899
BstMBI GATC 5 cut(s) 116, 372, 828, 1104, 1173
BstMCI CGRYCG 1 cut(s) 375
BstMWI GCNNNNNNNGC 7 cut(s) 19, 377, 514, 780, 986, 1028, 1306
BstNSI RCATGY 1 cut(s) 659
BstSCI CCNGG 2 cut(s) 1328, 1329
BstSLI GKGCMC 2 cut(s) 1067, 1330
BstV1I GCAGC 4 cut(s) 461, 535, 786, 790
BstV2I GAAGAC 2 cut(s) 1248, 1304
BstX2I RGATCY 1 cut(s) 1104
BstYI RGATCY 1 cut(s) 1104
BsuRI GGCC 2 cut(s) 193, 710
BtgI CCRYGG 1 cut(s) 694
BtsCI GGATG 6 cut(s) 345, 390, 531, 678, 991, 1273
BtsI GCAGTG 1 cut(s) 1152
BtsIMutI CAGTG 3 cut(s) 328, 1067, 1152
Cac8I GCNNGC 3 cut(s) 87, 260, 264
CaiI CAGNNNCTG 2 cut(s) 780, 1049
CfoI GCGC 4 cut(s) 85, 380, 822, 1211
Cfr13I GGNCC 3 cut(s) 537, 843, 1149
Cfr9I CCCGGG 1 cut(s) 1329
CseI GACGC 2 cut(s) 594, 1298
Csp6I GTAC 2 cut(s) 154, 653
CspCI CAANNNNNGTGG 2 cut(s) 292, 327
CviAII CATG 6 cut(s) 274, 433, 505, 656, 695, 1113
CviQI GTAC 2 cut(s) 154, 653
DdeI CTNAG 5 cut(s) 9, 133, 180, 320, 1042
DpnI GATC 5 cut(s) 118, 374, 830, 1106, 1175
DpnII GATC 5 cut(s) 116, 372, 828, 1104, 1173
EaeI YGGCCR 1 cut(s) 191
Eam1104I CTCTTC 2 cut(s) 395, 750
EarI CTCTTC 2 cut(s) 395, 750
Eco130I CCWWGG 2 cut(s) 253, 694
Eco147I AGGCCT 1 cut(s) 710
Eco24I GRGCYC 1 cut(s) 102
Eco47I GGWCC 3 cut(s) 537, 843, 1149
Eco47III AGCGCT 1 cut(s) 1210
Eco57I CTGAAG 1 cut(s) 498
Eco88I CYCGRG 2 cut(s) 1099, 1329
EcoRI GAATTC 1 cut(s) 1274
EcoT14I CCWWGG 2 cut(s) 253, 694
EcoT22I ATGCAT 1 cut(s) 502
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 2 cut(s) 253, 694
FaeI CATG 6 cut(s) 277, 436, 508, 659, 698, 1116
FaqI GGGAC 2 cut(s) 235, 1142
FatI CATG 6 cut(s) 273, 432, 504, 655, 694, 1112
FauNDI CATATG 1 cut(s) 496
FblI GTMKAC 3 cut(s) 472, 868, 1007
Fnu4HI GCNGC 6 cut(s) 6, 194, 450, 549, 775, 804
FokI GGATG 6 cut(s) 352, 397, 538, 685, 998, 1280
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 6 cut(s) 6, 194, 450, 549, 775, 804
GlaI GCGC 4 cut(s) 84, 379, 821, 1210
GluI GCNGC 6 cut(s) 6, 194, 450, 549, 775, 804
GsaI CCCAGC 1 cut(s) 384
GsuI CTGGAG 1 cut(s) 1032
HaeII RGCGCY 2 cut(s) 381, 1212
HaeIII GGCC 2 cut(s) 193, 710
HapII CCGG 2 cut(s) 1089, 1330
HgaI GACGC 2 cut(s) 594, 1298
HhaI GCGC 4 cut(s) 85, 380, 822, 1211
Hin1II CATG 6 cut(s) 277, 436, 508, 659, 698, 1116
Hin6I GCGC 4 cut(s) 83, 378, 820, 1209
HinP1I GCGC 4 cut(s) 83, 378, 820, 1209
HincII GTYRAC 1 cut(s) 1347
HindII GTYRAC 1 cut(s) 1347
HindIII AAGCTT 1 cut(s) 616
HinfI GANTC 6 cut(s) 58, 268, 469, 918, 1014, 1255
HpaII CCGG 2 cut(s) 1089, 1330
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 8 cut(s) 144, 473, 584, 791, 869, 1008, 1152, 1347
Hpy188I TCNGA 4 cut(s) 121, 168, 726, 1294
Hpy188III TCNNGA 5 cut(s) 298, 457, 704, 943, 1108
Hpy8I GTNNAC 8 cut(s) 144, 473, 584, 791, 869, 1008, 1152, 1347
HpyAV CCTTC 1 cut(s) 163
HpyCH4III ACNGT 4 cut(s) 226, 652, 1071, 1378
HpyCH4IV ACGT 1 cut(s) 939
HpyCH4V TGCA 6 cut(s) 292, 365, 500, 755, 1157, 1238
HpyF10VI GCNNNNNNNGC 7 cut(s) 19, 377, 514, 780, 986, 1028, 1306
HpyF3I CTNAG 5 cut(s) 9, 133, 180, 320, 1042
HpySE526I ACGT 1 cut(s) 939
Hsp92II CATG 6 cut(s) 277, 436, 508, 659, 698, 1116
HspAI GCGC 4 cut(s) 83, 378, 820, 1209
Kzo9I GATC 5 cut(s) 116, 372, 828, 1104, 1173
LguI GCTCTTC 1 cut(s) 395
LmnI GCTCC 1 cut(s) 343
Lsp1109I GCAGC 4 cut(s) 461, 535, 786, 790
LweI GCATC 5 cut(s) 375, 786, 976, 1258, 1287
MaeII ACGT 1 cut(s) 939
MaeIII GTNAC 4 cut(s) 15, 729, 757, 1213
MalI GATC 5 cut(s) 118, 374, 830, 1106, 1175
MboI GATC 5 cut(s) 116, 372, 828, 1104, 1173
MboII GAAGA 8 cut(s) 292, 343, 382, 737, 838, 1253, 1270, 1304
MflI RGATCY 1 cut(s) 1104
MhlI GDGCHC 4 cut(s) 102, 321, 1067, 1330
MluCI AATT 1 cut(s) 1274
MlyI GAGTC 2 cut(s) 478, 1023
MmeI TCCRAC 2 cut(s) 215, 1272
MnlI CCTC 9 cut(s) 71, 175, 480, 700, 721, 732, 899, 975, 1012
Mph1103I ATGCAT 1 cut(s) 502
MseI TTAA 4 cut(s) 111, 566, 924, 1119
MslI CAYNNNNRTG 4 cut(s) 22, 437, 1157, 1323
MspA1I CMGCKG 2 cut(s) 452, 803
MspI CCGG 2 cut(s) 1089, 1330
MspR9I CCNGG 2 cut(s) 1330, 1331
Mva1269I GAATGC 1 cut(s) 500
MwoI GCNNNNNNNGC 7 cut(s) 19, 377, 514, 780, 986, 1028, 1306
NciI CCSGG 2 cut(s) 1330, 1331
NcoI CCATGG 1 cut(s) 694
NdeI CATATG 1 cut(s) 496
NdeII GATC 5 cut(s) 116, 372, 828, 1104, 1173
NlaIII CATG 6 cut(s) 277, 436, 508, 659, 698, 1116
NlaIV GGNNCC 1 cut(s) 1150
NmuCI GTSAC 4 cut(s) 15, 729, 757, 1213
NsiI ATGCAT 1 cut(s) 502
NspI RCATGY 1 cut(s) 659
NspV TTCGAA 1 cut(s) 811
OliI CACNNNNGTG 1 cut(s) 22
PceI AGGCCT 1 cut(s) 710
PciI ACATGT 1 cut(s) 655
PciSI GCTCTTC 1 cut(s) 395
PcsI WCGNNNNNNNCGW 1 cut(s) 25
PctI GAATGC 1 cut(s) 500
PfeI GAWTC 4 cut(s) 58, 268, 918, 1255
PkrI GCNGC 6 cut(s) 7, 195, 451, 550, 776, 805
Ple19I CGATCG 1 cut(s) 375
PleI GAGTC 2 cut(s) 477, 1022
PpsI GAGTC 2 cut(s) 477, 1022
PscI ACATGT 1 cut(s) 655
PspFI CCCAGC 1 cut(s) 380
PspN4I GGNNCC 1 cut(s) 1150
PspPI GGNCC 3 cut(s) 537, 843, 1149
PstNI CAGNNNCTG 2 cut(s) 780, 1049
PsuI RGATCY 1 cut(s) 1104
PvuI CGATCG 1 cut(s) 375
PvuII CAGCTG 2 cut(s) 452, 803
RsaI GTAC 2 cut(s) 155, 654
RsaNI GTAC 2 cut(s) 154, 653
RseI CAYNNNNRTG 4 cut(s) 22, 437, 1157, 1323
SapI GCTCTTC 1 cut(s) 395
SaqAI TTAA 4 cut(s) 111, 566, 924, 1119
SatI GCNGC 6 cut(s) 6, 194, 450, 549, 775, 804
Sau3AI GATC 5 cut(s) 116, 372, 828, 1104, 1173
Sau96I GGNCC 3 cut(s) 537, 843, 1149
ScaI AGTACT 1 cut(s) 155
SchI GAGTC 2 cut(s) 478, 1023
ScrFI CCNGG 2 cut(s) 1330, 1331
SduI GDGCHC 4 cut(s) 102, 321, 1067, 1330
SfaNI GCATC 5 cut(s) 375, 786, 976, 1258, 1287
SfuI TTCGAA 1 cut(s) 811
SinI GGWCC 3 cut(s) 537, 843, 1149
SmaI CCCGGG 1 cut(s) 1331
SmiMI CAYNNNNRTG 4 cut(s) 22, 437, 1157, 1323
SrfI GCCCGGGC 1 cut(s) 1331
Sse9I AATT 1 cut(s) 1274
SseBI AGGCCT 1 cut(s) 710
SsiI CCGC 5 cut(s) 5, 22, 89, 194, 287
StuI AGGCCT 1 cut(s) 710
StyD4I CCNGG 2 cut(s) 1328, 1329
StyI CCWWGG 2 cut(s) 253, 694
TaaI ACNGT 4 cut(s) 226, 652, 1071, 1378
TaiI ACGT 1 cut(s) 942
TaqI TCGA 4 cut(s) 703, 811, 1017, 1380
TaqII GACCGA 1 cut(s) 831
TasI AATT 1 cut(s) 1274
TatI WGTACW 2 cut(s) 153, 652
TauI GCSGC 2 cut(s) 8, 196
TfiI GAWTC 4 cut(s) 58, 268, 918, 1255
Tru1I TTAA 4 cut(s) 111, 566, 924, 1119
Tru9I TTAA 4 cut(s) 111, 566, 924, 1119
TscAI CASTG 3 cut(s) 328, 1074, 1159
TseFI GTSAC 4 cut(s) 15, 729, 757, 1213
TseI GCWGC 4 cut(s) 449, 548, 774, 803
Tsp45I GTSAC 4 cut(s) 15, 729, 757, 1213
TspDTI ATGAA 6 cut(s) 50, 293, 493, 752, 884, 1247
TspMI CCCGGG 1 cut(s) 1329
TspRI CASTG 3 cut(s) 328, 1074, 1159
VpaK11BI GGWCC 3 cut(s) 537, 843, 1149
XapI RAATTY 1 cut(s) 1274
XceI RCATGY 1 cut(s) 659
XcmI CCANNNNNNNNNTGG 1 cut(s) 154
XmaI CCCGGG 1 cut(s) 1329
XmiI GTMKAC 3 cut(s) 472, 868, 1007
ZrmI AGTACT 1 cut(s) 155
Zsp2I ATGCAT 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.