MD16G1046200.v1.1

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007935
Physical Location & Seq
Reverse (-)
3211379 .. 3215454
4076 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1046200.v1.1.491

Sequence Viewer

Length: 1524 bp
ATGAACAACATCTCTCACAGCTTCTCCTCCTCCACCTCCTCCTCCGTCAATTCCGCCTCCATCACCACCGCTGATCCGAATGCAATCTCGAGTCGGAGGAGCATGGATGGTAGTCAAGCCTTGGCTGCTGAGCCATCGCGTCGATGGCGTGACATTTTCTGGTTGGGAGTGTTTCTAATTCATTTGGTTTTGGTGGGATGTGCACTTATAGTTCTGGGAATCAATAGGTTTAAGAAAACAGATAGGCTAAACATAGATAGGTACACCCAGAGGTTTTTGGAAAATCAAAGGGGGTTGACGGAGAACTATTGGCTGCTTTATGCTCTTGCCGGTGGAGTTGGGACACTTCTTGGTTGGACATGGTTGTTCTTCCTCCGTTCTCAAGCGAATCACATGATGAAGTTTGCGGTTCACATCTTGACTACTTATCTTGCTGTGATTAGTGTTCTATTGTTTTGGATGGAACAGTTTTTCTGGGGAGTTGCATTTGCGATTGGTGCTGTATTGCAGTTCATGTATGTTCTTTCAATTGTGGACAGGTTGCAGAGACTTCCCTTTACCATGCTGGTGCTACAAGCCGCTGTCAAGATGGTATGGAGTCTACGAGAAGTTATGAGAGTAGCATATGCTTTCATGCTTATTATGCTTGTTTGGATGGTATTATGGTCGTTTGGGGCAGCTGGTGTTGTAGCTTCAAGCATTGGAGATGGTGGACGCTGGTGGCTTCTTGTGGTTCTCTCTATAAGCTTATTTTGGACGGGGGCAGTACTCTGTAATACTGTGCATGTTATAGTGTCTGGGATGGTGTTTCTTGTTCTTATCCATGGTGGTAGGGAGGCACCATCAATGCCTCCAAACTCACTGATGAAGTCTCTTCGGTATGCAGTCACAACTTCTTTTGGCAGTATCTGCTATGGTTCACTTTTCACAGCTTTCATTCGGACACTGCGGTGGAAGATCAGGGGAGTTCGGTCCAAGATAGGCAACAATGAGTGTCTACTTTGCTGTGTTGATTTTCTCTTTAATCTAGTGGAGACTCTTGTCCGTTTCTTTAACAAGTATGCCTATGTCCAGATAGCTGTTTCTGGCAAAGGCTTCAATCATTCAGCGAGGGATGCCTGGGAGTTATTTCAATCTACTGGAGTGGAGGCTCTTGTTGCCTATGATTGTTCAGGTGCTGTTCTATTGATGGGAACTGTTTTGGGCGGGCTTATTACAGGAACATCCGCGGGGATCTGGACATGGATTAAGTATAGGGACAGAGTGGTTATGGTGGGGTCCACTGCAATGTTGATGGGAATGGTCTTGGTGGGGATAGCAATGGTGGTAGTGGAAGGTGCCGTTACATCTATTTATATCTGTTATGCAGAAGACCCCTTGTTGATTCACAGGTGGGATGCTGAGTTCTTCAACCAGATGTCAGAGATGCTACACCAGCGTCTTCAACATAGGAGCTCACGAGCGAGGGAAGTGTTAACCCACCATCGCTTTGATAACCACACACAAGAAACACTCCCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

508

Amino Acids

57.03

Weight (kDa)

9.43

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 167 - 459 1.2e-56 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 838, 1337
AccI GTMKAC 2 cut(s) 601, 997
AccII CGCG 2 cut(s) 139, 1229
AciI CCGC 8 cut(s) 54, 69, 407, 579, 949, 1206, 1227, 1229
AclWI GGATC 2 cut(s) 68, 1241
AfaI GTAC 2 cut(s) 263, 768
AgsI TTSAA 6 cut(s) 528, 696, 1099, 1133, 1411, 1445
AjnI CCWGG 1 cut(s) 1118
AleI CACNNNNGTG 1 cut(s) 949
AloI GAACNNNNNNTCC 2 cut(s) 1388, 1420
AluBI AGCT 7 cut(s) 21, 680, 692, 747, 932, 1079, 1455
AluI AGCT 7 cut(s) 21, 680, 692, 747, 932, 1079, 1455
Alw21I GWGCWC 2 cut(s) 205, 1457
Alw26I GTCTC 3 cut(s) 541, 876, 1028
Alw44I GTGCAC 1 cut(s) 201
AlwI GGATC 2 cut(s) 68, 1241
AlwNI CAGNNNCTG 2 cut(s) 909, 1178
Ama87I CYCGRG 1 cut(s) 88
ApaLI GTGCAC 1 cut(s) 201
ApeKI GCWGC 3 cut(s) 125, 313, 677
AspS9I GGNCC 2 cut(s) 972, 1278
AsuHPI GGTGA 1 cut(s) 55
AvaI CYCGRG 1 cut(s) 88
AvaII GGWCC 2 cut(s) 972, 1278
BaeGI GKGCMC 1 cut(s) 205
BanI GGYRCC 2 cut(s) 838, 1337
BanII GRGCYC 1 cut(s) 1457
BauI CACGAG 1 cut(s) 1458
BbsI GAAGAC 2 cut(s) 1377, 1433
Bbv12I GWGCWC 2 cut(s) 205, 1457
BbvI GCAGC 3 cut(s) 112, 300, 689
BceAI ACGGC 1 cut(s) 1325
BciT130I CCWGG 1 cut(s) 1120
BcoDI GTCTC 3 cut(s) 541, 876, 1028
BfaI CTAG 1 cut(s) 1028
BisI GCNGC 4 cut(s) 126, 314, 579, 678
BlpI GCTNAGC 1 cut(s) 129
BlsI GCNGC 4 cut(s) 127, 315, 580, 679
BmcAI AGTACT 1 cut(s) 768
Bme1390I CCNGG 1 cut(s) 1120
Bme18I GGWCC 2 cut(s) 972, 1278
BmeT110I CYCGRG 1 cut(s) 88
BmgT120I GGNCC 2 cut(s) 972, 1278
BmiI GGNNCC 3 cut(s) 840, 1279, 1339
BmrFI CCNGG 1 cut(s) 1120
BmsI GCATC 3 cut(s) 1105, 1387, 1416
BoxI GACNNNNGTC 1 cut(s) 1040
BpiI GAAGAC 2 cut(s) 1377, 1433
BpmI CTGGAG 1 cut(s) 1161
Bpu1102I GCTNAGC 1 cut(s) 129
BpuEI CTTGAG 1 cut(s) 366
BsaJI CCNNGG 4 cut(s) 120, 823, 1119, 1227
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bse118I RCCGGY 1 cut(s) 329
Bse1I ACTGG 1 cut(s) 1144
Bse3DI GCAATG 2 cut(s) 1293, 1326
BseBI CCWGG 1 cut(s) 1120
BseDI CCNNGG 4 cut(s) 120, 823, 1119, 1227
BseGI GGATG 8 cut(s) 112, 203, 465, 660, 807, 1120, 1223, 1402
BseMI GCAATG 2 cut(s) 1293, 1326
BseMII CTCAG 2 cut(s) 120, 1392
BseNI ACTGG 1 cut(s) 1144
BseRI GAGGAG 5 cut(s) 16, 19, 28, 31, 112
BseSI GKGCMC 1 cut(s) 205
BseXI GCAGC 3 cut(s) 112, 300, 689
Bsh1236I CGCG 2 cut(s) 139, 1229
BshNI GGYRCC 2 cut(s) 838, 1337
BsiHKAI GWGCWC 2 cut(s) 205, 1457
BsiHKCI CYCGRG 1 cut(s) 88
BsiSI CCGG 1 cut(s) 330
BslFI GGGAC 2 cut(s) 355, 1271
BsmAI GTCTC 3 cut(s) 541, 876, 1028
BsmFI GGGAC 2 cut(s) 355, 1271
BsmI GAATGC 1 cut(s) 85
BsoBI CYCGRG 1 cut(s) 88
Bsp1286I GDGCHC 2 cut(s) 205, 1457
Bsp143I GATC 3 cut(s) 73, 957, 1233
Bsp1720I GCTNAGC 1 cut(s) 129
Bsp19I CCATGG 1 cut(s) 823
BspACI CCGC 8 cut(s) 54, 69, 407, 579, 949, 1206, 1227, 1229
BspCNI CTCAG 2 cut(s) 121, 1393
BspFNI CGCG 2 cut(s) 139, 1229
BspLI GGNNCC 3 cut(s) 840, 1279, 1339
BspPI GGATC 2 cut(s) 68, 1241
BspT107I GGYRCC 2 cut(s) 838, 1337
BsrDI GCAATG 2 cut(s) 1293, 1326
BsrFI RCCGGY 1 cut(s) 329
BsrI ACTGG 1 cut(s) 1144
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 4 cut(s) 120, 823, 1119, 1227
BssMI GATC 3 cut(s) 73, 957, 1233
BssSI CACGAG 1 cut(s) 1458
BssT1I CCWWGG 2 cut(s) 120, 823
Bst2BI CACGAG 1 cut(s) 1458
Bst2UI CCWGG 1 cut(s) 1120
Bst4CI ACNGT 3 cut(s) 468, 781, 1198
Bst6I CTCTTC 1 cut(s) 879
BstAPI GCANNNNNTGC 1 cut(s) 909
BstC8I GCNNGC 1 cut(s) 1208
BstDEI CTNAG 2 cut(s) 129, 1401
BstDSI CCRYGG 2 cut(s) 823, 1227
BstF5I GGATG 8 cut(s) 112, 203, 465, 660, 807, 1120, 1223, 1402
BstFNI CGCG 2 cut(s) 139, 1229
BstKTI GATC 3 cut(s) 76, 960, 1236
BstMAI GTCTC 3 cut(s) 541, 876, 1028
BstMBI GATC 3 cut(s) 73, 957, 1233
BstMWI GCNNNNNNNGC 8 cut(s) 125, 145, 497, 643, 909, 1115, 1157, 1435
BstNI CCWGG 1 cut(s) 1120
BstNSI RCATGY 1 cut(s) 788
BstPAI GACNNNNGTC 1 cut(s) 1040
BstSCI CCNGG 1 cut(s) 1118
BstSLI GKGCMC 1 cut(s) 205
BstUI CGCG 2 cut(s) 139, 1229
BstV1I GCAGC 3 cut(s) 112, 300, 689
BstV2I GAAGAC 2 cut(s) 1377, 1433
BstX2I RGATCY 1 cut(s) 1233
BstYI RGATCY 1 cut(s) 1233
BtgI CCRYGG 2 cut(s) 823, 1227
BtgZI GCGATG 2 cut(s) 120, 1469
BtsCI GGATG 8 cut(s) 112, 203, 465, 660, 807, 1120, 1223, 1402
BtsI GCAGTG 2 cut(s) 944, 1281
BtsIMutI CAGTG 3 cut(s) 860, 944, 1281
Cac8I GCNNGC 1 cut(s) 1208
CaiI CAGNNNCTG 2 cut(s) 909, 1178
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 2 cut(s) 972, 1278
Cfr42I CCGCGG 1 cut(s) 1230
CseI GACGC 3 cut(s) 128, 723, 1427
Csp6I GTAC 2 cut(s) 262, 767
CviAII CATG 9 cut(s) 103, 360, 394, 514, 562, 634, 785, 824, 1242
CviQI GTAC 2 cut(s) 262, 767
DdeI CTNAG 2 cut(s) 129, 1401
DpnI GATC 3 cut(s) 75, 959, 1235
DpnII GATC 3 cut(s) 73, 957, 1233
Eam1104I CTCTTC 1 cut(s) 879
EarI CTCTTC 1 cut(s) 879
EciI GGCGGA 1 cut(s) 43
Ecl136II GAGCTC 1 cut(s) 1455
Eco130I CCWWGG 2 cut(s) 120, 823
Eco24I GRGCYC 1 cut(s) 1457
Eco47I GGWCC 2 cut(s) 972, 1278
Eco53kI GAGCTC 1 cut(s) 1455
Eco88I CYCGRG 1 cut(s) 88
EcoICRI GAGCTC 1 cut(s) 1455
EcoRII CCWGG 1 cut(s) 1118
EcoT14I CCWWGG 2 cut(s) 120, 823
EcoT38I GRGCYC 1 cut(s) 1457
ErhI CCWWGG 2 cut(s) 120, 823
FaeI CATG 9 cut(s) 106, 363, 397, 517, 565, 637, 788, 827, 1245
FaqI GGGAC 2 cut(s) 355, 1271
FatI CATG 9 cut(s) 102, 359, 393, 513, 561, 633, 784, 823, 1241
FauI CCCGC 2 cut(s) 1199, 1222
FauNDI CATATG 1 cut(s) 625
FblI GTMKAC 2 cut(s) 601, 997
Fnu4HI GCNGC 4 cut(s) 126, 314, 579, 678
FokI GGATG 8 cut(s) 119, 210, 472, 667, 814, 1127, 1210, 1409
FriOI GRGCYC 1 cut(s) 1457
Fsp4HI GCNGC 4 cut(s) 126, 314, 579, 678
FspBI CTAG 1 cut(s) 1028
GluI GCNGC 4 cut(s) 126, 314, 579, 678
GsuI CTGGAG 1 cut(s) 1161
HapII CCGG 1 cut(s) 330
HgaI GACGC 3 cut(s) 128, 723, 1427
Hin1II CATG 9 cut(s) 106, 363, 397, 517, 565, 637, 788, 827, 1245
HincII GTYRAC 2 cut(s) 297, 1476
HindII GTYRAC 2 cut(s) 297, 1476
HindIII AAGCTT 1 cut(s) 745
HinfI GANTC 6 cut(s) 91, 219, 388, 598, 1036, 1384
HpaI GTTAAC 1 cut(s) 1476
HpaII CCGG 1 cut(s) 330
HphI GGTGA 1 cut(s) 55
Hpy188I TCNGA 4 cut(s) 78, 96, 942, 1423
Hpy188III TCNNGA 6 cut(s) 88, 418, 586, 1072, 1237, 1458
Hpy99I CGWCG 1 cut(s) 144
HpyAV CCTTC 1 cut(s) 1328
HpyCH4III ACNGT 3 cut(s) 468, 781, 1198
HpyCH4V TGCA 9 cut(s) 83, 203, 485, 508, 544, 784, 884, 1286, 1367
HpyF10VI GCNNNNNNNGC 8 cut(s) 125, 145, 497, 643, 909, 1115, 1157, 1435
HpyF3I CTNAG 2 cut(s) 129, 1401
Hsp92II CATG 9 cut(s) 106, 363, 397, 517, 565, 637, 788, 827, 1245
KspAI GTTAAC 1 cut(s) 1476
KspI CCGCGG 1 cut(s) 1230
Kzo9I GATC 3 cut(s) 73, 957, 1233
LmnI GCTCC 2 cut(s) 99, 1452
Lsp1109I GCAGC 3 cut(s) 112, 300, 689
LweI GCATC 3 cut(s) 1105, 1387, 1416
MaeI CTAG 1 cut(s) 1028
MaeIII GTNAC 3 cut(s) 149, 886, 1342
MalI GATC 3 cut(s) 75, 959, 1235
MboI GATC 3 cut(s) 73, 957, 1233
MboII GAAGA 6 cut(s) 361, 866, 967, 1382, 1399, 1433
MfeI CAATTG 1 cut(s) 528
MflI RGATCY 1 cut(s) 1233
MhlI GDGCHC 2 cut(s) 205, 1457
MluCI AATT 3 cut(s) 49, 177, 528
MlyI GAGTC 3 cut(s) 100, 607, 1030
MmeI TCCRAC 2 cut(s) 74, 335
MseI TTAA 5 cut(s) 231, 1023, 1053, 1248, 1475
MslI CAYNNNNRTG 3 cut(s) 566, 949, 1286
MspA1I CMGCKG 4 cut(s) 71, 581, 680, 1229
MspI CCGG 1 cut(s) 330
MspR9I CCNGG 1 cut(s) 1120
MunI CAATTG 1 cut(s) 528
Mva1269I GAATGC 1 cut(s) 85
MvaI CCWGG 1 cut(s) 1120
MvnI CGCG 2 cut(s) 139, 1229
MwoI GCNNNNNNNGC 8 cut(s) 125, 145, 497, 643, 909, 1115, 1157, 1435
NcoI CCATGG 1 cut(s) 823
NdeI CATATG 1 cut(s) 625
NdeII GATC 3 cut(s) 73, 957, 1233
NlaIII CATG 9 cut(s) 106, 363, 397, 517, 565, 637, 788, 827, 1245
NlaIV GGNNCC 3 cut(s) 840, 1279, 1339
NmuCI GTSAC 2 cut(s) 149, 886
NspI RCATGY 1 cut(s) 788
OliI CACNNNNGTG 1 cut(s) 949
PaeR7I CTCGAG 1 cut(s) 88
PctI GAATGC 1 cut(s) 85
PfeI GAWTC 3 cut(s) 219, 388, 1384
PkrI GCNGC 4 cut(s) 127, 315, 580, 679
PleI GAGTC 3 cut(s) 99, 606, 1030
PpsI GAGTC 3 cut(s) 99, 606, 1030
PshAI GACNNNNGTC 1 cut(s) 1040
Psp124BI GAGCTC 1 cut(s) 1457
Psp6I CCWGG 1 cut(s) 1118
PspGI CCWGG 1 cut(s) 1118
PspN4I GGNNCC 3 cut(s) 840, 1279, 1339
PspPI GGNCC 2 cut(s) 972, 1278
PstNI CAGNNNCTG 2 cut(s) 909, 1178
PsuI RGATCY 1 cut(s) 1233
PvuII CAGCTG 1 cut(s) 680
RsaI GTAC 2 cut(s) 263, 768
RsaNI GTAC 2 cut(s) 262, 767
RseI CAYNNNNRTG 3 cut(s) 566, 949, 1286
SacI GAGCTC 1 cut(s) 1457
SacII CCGCGG 1 cut(s) 1230
SaqAI TTAA 5 cut(s) 231, 1023, 1053, 1248, 1475
SatI GCNGC 4 cut(s) 126, 314, 579, 678
Sau3AI GATC 3 cut(s) 73, 957, 1233
Sau96I GGNCC 2 cut(s) 972, 1278
ScaI AGTACT 1 cut(s) 768
SchI GAGTC 3 cut(s) 100, 607, 1030
ScrFI CCNGG 1 cut(s) 1120
SduI GDGCHC 2 cut(s) 205, 1457
SfaNI GCATC 3 cut(s) 1105, 1387, 1416
Sfr274I CTCGAG 1 cut(s) 88
Sfr303I CCGCGG 1 cut(s) 1230
SgrBI CCGCGG 1 cut(s) 1230
SinI GGWCC 2 cut(s) 972, 1278
SlaI CTCGAG 1 cut(s) 88
SmiMI CAYNNNNRTG 3 cut(s) 566, 949, 1286
SmlI CTYRAG 2 cut(s) 88, 381
SmoI CTYRAG 2 cut(s) 88, 381
Sse9I AATT 3 cut(s) 49, 177, 528
SsiI CCGC 8 cut(s) 54, 69, 407, 579, 949, 1206, 1227, 1229
SspMI CTAG 1 cut(s) 1028
SstI GAGCTC 1 cut(s) 1457
StyD4I CCNGG 1 cut(s) 1118
StyI CCWWGG 2 cut(s) 120, 823
TaaI ACNGT 3 cut(s) 468, 781, 1198
TaqI TCGA 2 cut(s) 89, 142
TaqII GACCGA 1 cut(s) 960
TasI AATT 3 cut(s) 49, 177, 528
TatI WGTACW 1 cut(s) 766
TauI GCSGC 1 cut(s) 581
TfiI GAWTC 3 cut(s) 219, 388, 1384
Tru1I TTAA 5 cut(s) 231, 1023, 1053, 1248, 1475
Tru9I TTAA 5 cut(s) 231, 1023, 1053, 1248, 1475
TscAI CASTG 3 cut(s) 867, 951, 1288
TseFI GTSAC 2 cut(s) 149, 886
TseI GCWGC 3 cut(s) 125, 313, 677
Tsp45I GTSAC 2 cut(s) 149, 886
TspDTI ATGAA 7 cut(s) 17, 170, 413, 502, 622, 881, 925
TspGWI ACGGA 4 cut(s) 34, 314, 365, 1034
TspRI CASTG 3 cut(s) 867, 951, 1288
VneI GTGCAC 1 cut(s) 201
VpaK11BI GGWCC 2 cut(s) 972, 1278
XceI RCATGY 1 cut(s) 788
XcmI CCANNNNNNNNNTGG 1 cut(s) 141
XhoI CTCGAG 1 cut(s) 88
XmiI GTMKAC 2 cut(s) 601, 997
XspI CTAG 1 cut(s) 1028
ZrmI AGTACT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.