Rorug04G0287200

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007935
Physical Location & Seq
Reverse (-)
46032710 .. 46033392
683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0287200.1

Sequence Viewer

Length: 528 bp
ATGATGTTCACTGGCAACCCGCTTATGCTTTGCCTCTTCAGTTATTTTCTAAGTCCAAGCCATGAGCCTGAGAAGCTTCCTTTCGTCCTCCCCGCCGTCTTCACCATCGGTCCACGCGACGATGTGGAGGCCCTCCAGAACTACACCAAGCTCATTTCCAACCACGACAAGAACTCCAACCATGTCAAGGAGCTCATCCAGGGCATCATCGAGGGCGAGACACGTGTGCTCACAGCCTCGATGACGATGGAGGAGGTCTTCAAAGGGACCAAAGAGTTCAAGAAGGAGGTGTTTGAGAAGGTTCAGCTGGAGTTGGATCAGTTCGGGCTTTTGATATACAATGCCAATGTTAAACAATTGGTGGACGTTAGGGGACATGAGTATTTCGGTTACTTGGGACAGATGATTCAGATGGAGGCTGCCAACCAGGCCAAGGTTGATGTGGCCGAGGCCAAAATGAAGGGAGAGGTTGGCTCTAAGATGAGGGAGGGGCAGACATTGCAAAACGGAGGCAAGATCGACTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

175

Amino Acids

19.74

Weight (kDa)

5.43

Isoelectric Point (pI)

20.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 26 - 152 2.1e-13 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 117
AciI CCGC 2 cut(s) 20, 93
AclWI GGATC 1 cut(s) 324
AcoI YGGCCR 1 cut(s) 444
AcuI CTGAAG 1 cut(s) 22
AcvI CACGTG 1 cut(s) 224
AfiI CCNNNNNNNGG 2 cut(s) 187, 433
AflIII ACRYGT 2 cut(s) 221, 223
AgsI TTSAA 2 cut(s) 262, 280
AjnI CCWGG 2 cut(s) 198, 426
AluBI AGCT 4 cut(s) 76, 151, 193, 307
AluI AGCT 4 cut(s) 76, 151, 193, 307
Alw21I GWGCWC 2 cut(s) 195, 231
Alw26I GTCTC 1 cut(s) 212
AlwI GGATC 1 cut(s) 324
AoxI GGCC 4 cut(s) 129, 429, 444, 450
ApeKI GCWGC 1 cut(s) 419
AspS9I GGNCC 3 cut(s) 110, 130, 267
AsuHPI GGTGA 1 cut(s) 94
AvaII GGWCC 2 cut(s) 110, 267
BanII GRGCYC 1 cut(s) 195
BbrPI CACGTG 1 cut(s) 224
BbsI GAAGAC 2 cut(s) 91, 250
Bbv12I GWGCWC 2 cut(s) 195, 231
BbvI GCAGC 1 cut(s) 406
BccI CCATC 3 cut(s) 113, 241, 406
BceAI ACGGC 1 cut(s) 80
BciT130I CCWGG 2 cut(s) 200, 428
BcoDI GTCTC 1 cut(s) 212
BfaI CTAG 1 cut(s) 526
BglI GCCNNNNNGGC 1 cut(s) 428
BisI GCNGC 1 cut(s) 420
BlsI GCNGC 1 cut(s) 421
Bme1390I CCNGG 2 cut(s) 200, 428
Bme18I GGWCC 2 cut(s) 110, 267
BmgT120I GGNCC 3 cut(s) 110, 130, 267
BmiI GGNNCC 1 cut(s) 268
BmrFI CCNGG 2 cut(s) 200, 428
BmsI GCATC 1 cut(s) 213
BpiI GAAGAC 2 cut(s) 91, 250
BplI GAGNNNNNCTC 2 cut(s) 458, 490
BpmI CTGGAG 2 cut(s) 119, 329
BsaAI YACGTR 1 cut(s) 224
BsaJI CCNNGG 3 cut(s) 199, 432, 447
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 2 cut(s) 187, 433
Bse1I ACTGG 1 cut(s) 16
Bse3DI GCAATG 1 cut(s) 497
BseBI CCWGG 2 cut(s) 200, 428
BseDI CCNNGG 3 cut(s) 199, 432, 447
BseGI GGATG 1 cut(s) 195
BseLI CCNNNNNNNGG 2 cut(s) 187, 433
BseMI GCAATG 1 cut(s) 497
BseMII CTCAG 1 cut(s) 60
BseNI ACTGG 1 cut(s) 16
BseRI GAGGAG 1 cut(s) 266
BseXI GCAGC 1 cut(s) 406
Bsh1236I CGCG 1 cut(s) 117
BshFI GGCC 4 cut(s) 131, 431, 446, 452
BsiHKAI GWGCWC 2 cut(s) 195, 231
BslFI GGGAC 3 cut(s) 280, 387, 411
BslI CCNNNNNNNGG 2 cut(s) 187, 433
BsmAI GTCTC 1 cut(s) 212
BsmFI GGGAC 3 cut(s) 280, 387, 411
BsnI GGCC 4 cut(s) 131, 431, 446, 452
Bsp1286I GDGCHC 2 cut(s) 195, 231
Bsp143I GATC 2 cut(s) 316, 516
BspACI CCGC 2 cut(s) 20, 93
BspANI GGCC 4 cut(s) 131, 431, 446, 452
BspCNI CTCAG 1 cut(s) 61
BspFNI CGCG 1 cut(s) 117
BspLI GGNNCC 1 cut(s) 268
BspPI GGATC 1 cut(s) 324
BsrDI GCAATG 1 cut(s) 497
BsrI ACTGG 1 cut(s) 16
BssECI CCNNGG 3 cut(s) 199, 432, 447
BssMI GATC 2 cut(s) 316, 516
BssT1I CCWWGG 1 cut(s) 432
Bst2UI CCWGG 2 cut(s) 200, 428
Bst6I CTCTTC 1 cut(s) 41
BstAPI GCANNNNNTGC 1 cut(s) 499
BstBAI YACGTR 1 cut(s) 224
BstDEI CTNAG 3 cut(s) 50, 69, 477
BstF5I GGATG 1 cut(s) 195
BstFNI CGCG 1 cut(s) 117
BstKTI GATC 2 cut(s) 319, 519
BstMAI GTCTC 1 cut(s) 212
BstMBI GATC 2 cut(s) 316, 516
BstMWI GCNNNNNNNGC 3 cut(s) 73, 428, 499
BstNI CCWGG 2 cut(s) 200, 428
BstSCI CCNGG 2 cut(s) 198, 426
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 1 cut(s) 406
BstV2I GAAGAC 2 cut(s) 91, 250
BsuRI GGCC 4 cut(s) 131, 431, 446, 452
BtsCI GGATG 1 cut(s) 195
BtsIMutI CAGTG 1 cut(s) 9
Cfr13I GGNCC 3 cut(s) 110, 130, 267
CviAII CATG 3 cut(s) 62, 182, 377
DdeI CTNAG 3 cut(s) 50, 69, 477
DpnI GATC 2 cut(s) 318, 518
DpnII GATC 2 cut(s) 316, 516
EaeI YGGCCR 1 cut(s) 444
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Ecl136II GAGCTC 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 432
Eco24I GRGCYC 1 cut(s) 195
Eco47I GGWCC 2 cut(s) 110, 267
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 22
Eco72I CACGTG 1 cut(s) 224
EcoICRI GAGCTC 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 130
EcoRII CCWGG 2 cut(s) 198, 426
EcoT14I CCWWGG 1 cut(s) 432
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 3 cut(s) 65, 185, 380
FaiI YATR 5 cut(s) 26, 63, 183, 337, 378
FaqI GGGAC 3 cut(s) 280, 387, 411
FatI CATG 3 cut(s) 61, 181, 376
FauI CCCGC 2 cut(s) 27, 100
Fnu4HI GCNGC 1 cut(s) 420
FokI GGATG 1 cut(s) 182
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 1 cut(s) 420
FspBI CTAG 1 cut(s) 526
GluI GCNGC 1 cut(s) 420
GsuI CTGGAG 2 cut(s) 119, 329
HaeIII GGCC 4 cut(s) 131, 431, 446, 452
Hin1II CATG 3 cut(s) 65, 185, 380
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 2 cut(s) 406, 521
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 3 cut(s) 9, 113, 364
Hpy188I TCNGA 1 cut(s) 411
Hpy188III TCNNGA 2 cut(s) 136, 280
Hpy8I GTNNAC 3 cut(s) 9, 113, 364
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 3 cut(s) 277, 292, 454
HpyCH4IV ACGT 2 cut(s) 223, 366
HpyCH4V TGCA 1 cut(s) 502
HpyF10VI GCNNNNNNNGC 3 cut(s) 73, 428, 499
HpyF3I CTNAG 3 cut(s) 50, 69, 477
HpySE526I ACGT 2 cut(s) 223, 366
Hsp92II CATG 3 cut(s) 65, 185, 380
Kzo9I GATC 2 cut(s) 316, 516
LmnI GCTCC 1 cut(s) 190
LpnPI CCDG 7 cut(s) 81, 149, 185, 212, 293, 413, 440
Lsp1109I GCAGC 1 cut(s) 406
LweI GCATC 1 cut(s) 213
MaeI CTAG 1 cut(s) 526
MaeII ACGT 2 cut(s) 223, 366
MaeIII GTNAC 1 cut(s) 389
MalI GATC 2 cut(s) 318, 518
MboI GATC 2 cut(s) 316, 516
MboII GAAGA 3 cut(s) 28, 91, 250
MfeI CAATTG 1 cut(s) 356
MhlI GDGCHC 2 cut(s) 195, 231
MluCI AATT 1 cut(s) 356
MlyI GAGTC 1 cut(s) 515
MmeI TCCRAC 3 cut(s) 183, 201, 294
MseI TTAA 1 cut(s) 351
MspA1I CMGCKG 1 cut(s) 307
MspR9I CCNGG 2 cut(s) 200, 428
MunI CAATTG 1 cut(s) 356
MvaI CCWGG 2 cut(s) 200, 428
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 3 cut(s) 73, 428, 499
NdeII GATC 2 cut(s) 316, 516
NlaIII CATG 3 cut(s) 65, 185, 380
NlaIV GGNNCC 1 cut(s) 268
NmeAIII GCCGAG 1 cut(s) 472
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PfeI GAWTC 1 cut(s) 406
PkrI GCNGC 1 cut(s) 421
PleI GAGTC 1 cut(s) 515
PmaCI CACGTG 1 cut(s) 224
PmlI CACGTG 1 cut(s) 224
PpsI GAGTC 1 cut(s) 515
Ppu21I YACGTR 1 cut(s) 224
Psp124BI GAGCTC 1 cut(s) 195
Psp6I CCWGG 2 cut(s) 198, 426
PspCI CACGTG 1 cut(s) 224
PspGI CCWGG 2 cut(s) 198, 426
PspN4I GGNNCC 1 cut(s) 268
PspPI GGNCC 3 cut(s) 110, 130, 267
PvuII CAGCTG 1 cut(s) 307
SacI GAGCTC 1 cut(s) 195
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 1 cut(s) 420
Sau3AI GATC 2 cut(s) 316, 516
Sau96I GGNCC 3 cut(s) 110, 130, 267
SchI GAGTC 1 cut(s) 515
ScrFI CCNGG 2 cut(s) 200, 428
SduI GDGCHC 2 cut(s) 195, 231
SfaNI GCATC 1 cut(s) 213
SinI GGWCC 2 cut(s) 110, 267
Sse9I AATT 1 cut(s) 356
SsiI CCGC 2 cut(s) 20, 93
SspMI CTAG 1 cut(s) 526
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 2 cut(s) 198, 426
StyI CCWWGG 1 cut(s) 432
TaiI ACGT 2 cut(s) 226, 369
TaqI TCGA 3 cut(s) 210, 239, 519
TaqII GACCGA 1 cut(s) 98
TasI AATT 1 cut(s) 356
TfiI GAWTC 1 cut(s) 406
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 1 cut(s) 16
TseI GCWGC 1 cut(s) 419
TspDTI ATGAA 1 cut(s) 473
TspGWI ACGGA 1 cut(s) 522
TspRI CASTG 1 cut(s) 16
VpaK11BI GGWCC 2 cut(s) 110, 267
XcmI CCANNNNNNNNNTGG 1 cut(s) 439
XspI CTAG 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.