AT1G27920

Microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007124
Physical Location & Seq
Forward (+)
9726655 .. 9729848
3194 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27920.1

Sequence Viewer

Length: 1689 bp
ATGGGTTCGCTTCAGACACCGATAGAAATGAGAAGCTCATCGTTGTTGGATACGTCCTGCGGATATTTGCTCAGAGAGTTACAGATGATATGGGATGAAGTTGGAGAAGATAAGTTTGAGAGAGAAAAAGTGTTGCTTGATATAGAGCAGGAATGTGTAGAGGCTTACCGGAGAAAAGTTGACCATGCCAATGTTTCGAGGTCTCGTCTACATCAAGAGCTGGCAGAATCTGAAGCCGAACTCACCCATTTTCTTTTGTGCCTCGGTGAAAGATCAGTACCTGGAAGACCGGAGAAAAAGGGAGGAACACTGAGGGAGCAGCTGGATTCTATTGCGCCTGCACTGCGTGAGATGAGGCTTAGGAAAGACGAGAGAGTGAAGCAGTTCCGATCTGTCAAAGGGGAAATTCAGAAAATATCAGCAGAAATAGCAGGTAGATCGACATATGAAGACTCAACTAGAAAGATAACCATAGATGACAACGATCTTTCTAATAAAAAACTTGAGGAATATCAGAATGAACTGCATAGACTCCATGATGAGAAGAATGAGAGACTACAGAAGGTTGATATATACATATGCGCCATTCGAGATCTATCAGCAACATTGGGAACAGAAGCATCAATGATCATAACAAAGATTCACCCAAGCTTGAACGACCTCTATGGAATATCCAAAAACATCAGTGATGATATCTTAAAAAAGCTTAATGGCACTGTTGTGTCTCTGGAAGAGGAAAAGCACAAGCGCCTTGAAAAGCTTCACCATCTTGGTAGAGCTTTATCGAACCTGTGGAATCTGATGGATGCATCTTATGAAGATCGTCAAAAGTTTTTTCATGTCATTGATTTACTGTCGTCCGCACCATCAGACGTGTGTGCTCCTGGAAGCATTACATTGGACATAATTCAGCAGGCTGAGGCTGAAGTCAAGAGACTAGATCAACTGAAAGCAAGCAGAATCAAAGAACTTTTCATCAAGAAGCAAAAGGAACTAGAAGATACGTGCAACATGTCGCATATGGAGACTCCATCAACGGAAATGGGAAATATCACGAACCTTGTAGACTCTGGGGAAGTTGACCATGTTGACCTTCTAGCCGCCATGGATGAAAAGATAGCAAGAGCAAAGGAAGAAGCAGCAAGTAGAAAAGGAATAATTGAAAAAGTTGATAGGTGGATGTTGGCAAGTGATGAAGAGCGCTGGCTAGAAGAATATGACCAGGACGAGAATCGGTACTCAGTAAGTAGAAATGCTCATCGAAACCTAAGGAGAGCAGAACGGGCACGCATAACAGTCAGCAAAATCTCAGGATTAGTGGAATCTATATTGGTTAAGGCTAAGAGCTGGGAGGTAGAAAGACAGAAAGTCTTCTTATACAATGAGGTACCTCTTGTTGCAATGTTGCAAGAATACAATAAGCTGAGGCAGGAAAAAGAAATGGAGAAGCAAAGGCTCCGGGAAATGAAGAAGATGAGCATTCCACAACCTGTGGCTGAGGGAGATAATTTTTACATGGCAAGGCCAGCTAGCAGCAATAGGCGAATATCAAATCGAAGCATGAATGGTGGATTTGGAAGTGGAAGTCCTATAAACAGAAAGTATTCAGGAGGATTCAACAATACTAACAATAATTACACAGCACTTGGTACATCTATAAGAAGAGAAAGCAGAAAGAGTGAGGCATAA

Protein Analysis

562

Amino Acids

64.6

Weight (kDa)

6.42

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 23 - 493 1.3e-61 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 422
Acc65I GGTACC 1 cut(s) 1387
AccB1I GGYRCC 1 cut(s) 1387
AccI GTMKAC 2 cut(s) 208, 1065
AciI CCGC 3 cut(s) 60, 861, 1101
AcsI RAATTY 1 cut(s) 405
AcuI CTGAAG 2 cut(s) 252, 945
AdeI CACNNNGTG 1 cut(s) 347
AfaI GTAC 4 cut(s) 279, 1238, 1389, 1651
AfeI AGCGCT 1 cut(s) 1202
AflIII ACRYGT 2 cut(s) 873, 1011
AgsI TTSAA 4 cut(s) 655, 755, 1163, 1618
AhdI GACNNNNNGTC 1 cut(s) 1367
AjiI CACGTC 1 cut(s) 874
AjnI CCWGG 3 cut(s) 280, 883, 1221
AleI CACNNNNGTG 1 cut(s) 719
Alw21I GWGCWC 1 cut(s) 883
Alw26I GTCTC 5 cut(s) 207, 547, 729, 928, 1019
AlwNI CAGNNNCTG 2 cut(s) 230, 281
Aor51HI AGCGCT 1 cut(s) 1202
AoxI GGCC 1 cut(s) 1523
ApeKI GCWGC 3 cut(s) 319, 1139, 1533
ApoI RAATTY 1 cut(s) 405
Asp700I GAANNNNTTC 4 cut(s) 383, 759, 1370, 1603
Asp718I GGTACC 1 cut(s) 1387
AspLEI GCGC 4 cut(s) 337, 584, 750, 1203
AsuC2I CCSGG 1 cut(s) 1460
AsuHPI GGTGA 4 cut(s) 235, 278, 635, 755
AsuNHI GCTAGC 1 cut(s) 1529
AxyI CCTNAGG 1 cut(s) 1268
BaeGI GKGCMC 1 cut(s) 1288
BanI GGYRCC 1 cut(s) 1387
BbsI GAAGAC 3 cut(s) 292, 456, 1363
Bbv12I GWGCWC 1 cut(s) 883
BbvCI CCTCAGC 3 cut(s) 918, 1424, 1497
BbvI GCAGC 3 cut(s) 331, 1151, 1545
BccI CCATC 4 cut(s) 774, 796, 874, 1039
BcgI CGANNNNNNTGC 2 cut(s) 420, 454
BciT130I CCWGG 3 cut(s) 282, 885, 1223
BciVI GTATCC 1 cut(s) 43
BclI TGATCA 1 cut(s) 627
BcnI CCSGG 1 cut(s) 1460
BcoDI GTCTC 5 cut(s) 207, 547, 729, 928, 1019
BfaI CTAG 6 cut(s) 459, 938, 995, 1097, 1208, 1530
BfmI CTRYAG 1 cut(s) 557
BfoI RGCGCY 2 cut(s) 751, 1204
BfuAI ACCTGC 1 cut(s) 422
BfuI GTATCC 1 cut(s) 43
BglII AGATCT 1 cut(s) 592
BisI GCNGC 4 cut(s) 320, 1101, 1140, 1534
BlsI GCNGC 4 cut(s) 321, 1102, 1141, 1535
Bme1390I CCNGG 4 cut(s) 282, 885, 1223, 1460
BmeRI GACNNNNNGTC 1 cut(s) 1367
BmgBI CACGTC 1 cut(s) 874
BmiI GGNNCC 2 cut(s) 1389, 1457
BmrFI CCNGG 4 cut(s) 282, 885, 1223, 1460
BmsI GCATC 3 cut(s) 629, 796, 818
BmtI GCTAGC 1 cut(s) 1533
BpiI GAAGAC 3 cut(s) 292, 456, 1363
Bpu10I CCTNAGC 4 cut(s) 359, 918, 1424, 1497
BpuEI CTTGAG 1 cut(s) 524
BpuMI CCSGG 1 cut(s) 1460
BsaAI YACGTR 1 cut(s) 1005
BsaI GGTCTC 1 cut(s) 207
BsaJI CCNNGG 2 cut(s) 262, 1104
BsaWI WCCGGW 2 cut(s) 168, 289
Bse21I CCTNAGG 1 cut(s) 1268
Bse3DI GCAATG 1 cut(s) 1407
BseBI CCWGG 3 cut(s) 282, 885, 1223
BseDI CCNNGG 2 cut(s) 262, 1104
BseGI GGATG 4 cut(s) 100, 811, 1114, 1185
BseMI GCAATG 1 cut(s) 1407
BseMII CTCAG 7 cut(s) 85, 302, 909, 1254, 1323, 1415, 1488
BseSI GKGCMC 1 cut(s) 1288
BseXI GCAGC 3 cut(s) 331, 1151, 1545
BseYI CCCAGC 1 cut(s) 1347
BsgI GTGCAG 1 cut(s) 324
BshFI GGCC 1 cut(s) 1525
BshNI GGYRCC 1 cut(s) 1387
BsiHKAI GWGCWC 1 cut(s) 883
BsiSI CCGG 3 cut(s) 169, 290, 1459
BsmAI GTCTC 5 cut(s) 207, 547, 729, 928, 1019
BsmI GAATGC 1 cut(s) 1479
BsnI GGCC 1 cut(s) 1525
Bso31I GGTCTC 1 cut(s) 207
Bsp1286I GDGCHC 2 cut(s) 883, 1288
Bsp143I GATC 8 cut(s) 272, 389, 437, 484, 592, 627, 820, 940
Bsp19I CCATGG 1 cut(s) 1104
BspACI CCGC 3 cut(s) 60, 861, 1101
BspANI GGCC 1 cut(s) 1525
BspCNI CTCAG 7 cut(s) 84, 303, 910, 1253, 1322, 1416, 1489
BspLI GGNNCC 2 cut(s) 1389, 1457
BspMI ACCTGC 1 cut(s) 422
BspOI GCTAGC 1 cut(s) 1533
BspQI GCTCTTC 1 cut(s) 1191
BspT107I GGYRCC 1 cut(s) 1387
BspTNI GGTCTC 1 cut(s) 207
BsrDI GCAATG 1 cut(s) 1407
BssECI CCNNGG 2 cut(s) 262, 1104
BssMI GATC 8 cut(s) 272, 389, 437, 484, 592, 627, 820, 940
BssT1I CCWWGG 1 cut(s) 1104
Bst2UI CCWGG 3 cut(s) 282, 885, 1223
Bst4CI ACNGT 3 cut(s) 718, 855, 1297
Bst6I CTCTTC 3 cut(s) 726, 1191, 1657
BstBAI YACGTR 1 cut(s) 1005
BstC8I GCNNGC 8 cut(s) 222, 339, 915, 955, 1205, 1288, 1527, 1531
BstDSI CCRYGG 1 cut(s) 1104
BstF5I GGATG 4 cut(s) 100, 811, 1114, 1185
BstH2I RGCGCY 2 cut(s) 751, 1204
BstHHI GCGC 4 cut(s) 337, 584, 750, 1203
BstKTI GATC 8 cut(s) 275, 392, 440, 487, 595, 630, 823, 943
BstMAI GTCTC 5 cut(s) 207, 547, 729, 928, 1019
BstMBI GATC 8 cut(s) 272, 389, 437, 484, 592, 627, 820, 940
BstMWI GCNNNNNNNGC 4 cut(s) 343, 428, 1283, 1526
BstNI CCWGG 3 cut(s) 282, 885, 1223
BstNSI RCATGY 1 cut(s) 1015
BstSCI CCNGG 4 cut(s) 280, 883, 1221, 1458
BstSFI CTRYAG 1 cut(s) 557
BstSLI GKGCMC 1 cut(s) 1288
BstV1I GCAGC 3 cut(s) 331, 1151, 1545
BstV2I GAAGAC 3 cut(s) 292, 456, 1363
BstX2I RGATCY 1 cut(s) 592
BstYI RGATCY 1 cut(s) 592
Bsu36I CCTNAGG 1 cut(s) 1268
BsuI GTATCC 1 cut(s) 43
BsuRI GGCC 1 cut(s) 1525
BtgI CCRYGG 1 cut(s) 1104
BtrI CACGTC 1 cut(s) 874
BtsCI GGATG 4 cut(s) 100, 811, 1114, 1185
BtsI GCAGTG 1 cut(s) 341
BtsIMutI CAGTG 4 cut(s) 308, 341, 691, 714
BveI ACCTGC 1 cut(s) 422
Cac8I GCNNGC 8 cut(s) 222, 339, 915, 955, 1205, 1288, 1527, 1531
CaiI CAGNNNCTG 2 cut(s) 230, 281
CfoI GCGC 4 cut(s) 337, 584, 750, 1203
Csp6I GTAC 4 cut(s) 278, 1237, 1388, 1650
CviAII CATG 8 cut(s) 185, 536, 839, 1012, 1085, 1105, 1516, 1561
CviQI GTAC 4 cut(s) 278, 1237, 1388, 1650
DpnI GATC 8 cut(s) 274, 391, 439, 486, 594, 629, 822, 942
DpnII GATC 8 cut(s) 272, 389, 437, 484, 592, 627, 820, 940
DraIII CACNNNGTG 1 cut(s) 347
DriI GACNNNNNGTC 1 cut(s) 1367
Eam1104I CTCTTC 3 cut(s) 726, 1191, 1657
Eam1105I GACNNNNNGTC 1 cut(s) 1367
EarI CTCTTC 3 cut(s) 726, 1191, 1657
Eco130I CCWWGG 1 cut(s) 1104
Eco31I GGTCTC 1 cut(s) 207
Eco32I GATATC 1 cut(s) 694
Eco47III AGCGCT 1 cut(s) 1202
Eco57I CTGAAG 2 cut(s) 252, 945
Eco81I CCTNAGG 1 cut(s) 1268
EcoRII CCWGG 3 cut(s) 280, 883, 1221
EcoRV GATATC 1 cut(s) 694
EcoT14I CCWWGG 1 cut(s) 1104
EcoT22I ATGCAT 1 cut(s) 811
ErhI CCWWGG 1 cut(s) 1104
FaeI CATG 8 cut(s) 188, 539, 842, 1015, 1088, 1108, 1519, 1564
FalI AAGNNNNNCTT 2 cut(s) 120, 152
FatI CATG 8 cut(s) 184, 535, 838, 1011, 1084, 1104, 1515, 1560
FauNDI CATATG 3 cut(s) 445, 578, 1020
FbaI TGATCA 1 cut(s) 627
FblI GTMKAC 2 cut(s) 208, 1065
Fnu4HI GCNGC 4 cut(s) 320, 1101, 1140, 1534
FokI GGATG 4 cut(s) 107, 818, 1121, 1192
Fsp4HI GCNGC 4 cut(s) 320, 1101, 1140, 1534
FspBI CTAG 6 cut(s) 459, 938, 995, 1097, 1208, 1530
GlaI GCGC 4 cut(s) 336, 583, 749, 1202
GluI GCNGC 4 cut(s) 320, 1101, 1140, 1534
GsaI CCCAGC 1 cut(s) 1351
HaeII RGCGCY 2 cut(s) 751, 1204
HaeIII GGCC 1 cut(s) 1525
HapII CCGG 3 cut(s) 169, 290, 1459
HhaI GCGC 4 cut(s) 337, 584, 750, 1203
Hin1II CATG 8 cut(s) 188, 539, 842, 1015, 1088, 1108, 1519, 1564
Hin6I GCGC 4 cut(s) 335, 582, 748, 1201
HinP1I GCGC 4 cut(s) 335, 582, 748, 1201
HincII GTYRAC 3 cut(s) 181, 1081, 1090
HindII GTYRAC 3 cut(s) 181, 1081, 1090
HindIII AAGCTT 3 cut(s) 649, 704, 758
HpaII CCGG 3 cut(s) 169, 290, 1459
HphI GGTGA 4 cut(s) 235, 278, 635, 755
Hpy166II GTNNAC 5 cut(s) 181, 209, 1066, 1081, 1090
Hpy188I TCNGA 8 cut(s) 15, 74, 232, 389, 411, 516, 801, 871
Hpy188III TCNNGA 8 cut(s) 215, 590, 728, 931, 979, 1054, 1311, 1608
Hpy8I GTNNAC 5 cut(s) 181, 209, 1066, 1081, 1090
HpyAV CCTTC 2 cut(s) 556, 1103
HpyCH4III ACNGT 3 cut(s) 718, 855, 1297
HpyCH4IV ACGT 3 cut(s) 53, 873, 1004
HpyCH4V TGCA 6 cut(s) 341, 526, 809, 1008, 1400, 1408
HpyF10VI GCNNNNNNNGC 4 cut(s) 343, 428, 1283, 1526
HpySE526I ACGT 3 cut(s) 53, 873, 1004
Hsp92II CATG 8 cut(s) 188, 539, 842, 1015, 1088, 1108, 1519, 1564
HspAI GCGC 4 cut(s) 335, 582, 748, 1201
KpnI GGTACC 1 cut(s) 1391
Ksp22I TGATCA 1 cut(s) 627
Kzo9I GATC 8 cut(s) 272, 389, 437, 484, 592, 627, 820, 940
LguI GCTCTTC 1 cut(s) 1191
LmnI GCTCC 3 cut(s) 316, 886, 1461
Lsp1109I GCAGC 3 cut(s) 331, 1151, 1545
LweI GCATC 3 cut(s) 629, 796, 818
MaeI CTAG 6 cut(s) 459, 938, 995, 1097, 1208, 1530
MaeII ACGT 3 cut(s) 53, 873, 1004
MaeIII GTNAC 1 cut(s) 78
MalI GATC 8 cut(s) 274, 391, 439, 486, 594, 629, 822, 942
MboI GATC 8 cut(s) 272, 389, 437, 484, 592, 627, 820, 940
MflI RGATCY 1 cut(s) 592
MhlI GDGCHC 2 cut(s) 883, 1288
MluCI AATT 5 cut(s) 405, 906, 1158, 1507, 1633
MlyI GAGTC 4 cut(s) 446, 525, 1021, 1061
MmeI TCCRAC 2 cut(s) 27, 82
Mph1103I ATGCAT 1 cut(s) 811
MroXI GAANNNNTTC 4 cut(s) 383, 759, 1370, 1603
MseI TTAA 3 cut(s) 698, 708, 1335
MslI CAYNNNNRTG 2 cut(s) 189, 719
MspA1I CMGCKG 1 cut(s) 322
MspI CCGG 3 cut(s) 169, 290, 1459
MspR9I CCNGG 4 cut(s) 282, 885, 1223, 1460
Mva1269I GAATGC 1 cut(s) 1479
MvaI CCWGG 3 cut(s) 282, 885, 1223
MwoI GCNNNNNNNGC 4 cut(s) 343, 428, 1283, 1526
NciI CCSGG 1 cut(s) 1460
NcoI CCATGG 1 cut(s) 1104
NdeI CATATG 3 cut(s) 445, 578, 1020
NdeII GATC 8 cut(s) 272, 389, 437, 484, 592, 627, 820, 940
NheI GCTAGC 1 cut(s) 1529
NlaIII CATG 8 cut(s) 188, 539, 842, 1015, 1088, 1108, 1519, 1564
NlaIV GGNNCC 2 cut(s) 1389, 1457
NsiI ATGCAT 1 cut(s) 811
NspI RCATGY 1 cut(s) 1015
OliI CACNNNNGTG 1 cut(s) 719
PciI ACATGT 1 cut(s) 1011
PciSI GCTCTTC 1 cut(s) 1191
PctI GAATGC 1 cut(s) 1479
PdmI GAANNNNTTC 4 cut(s) 383, 759, 1370, 1603
PfeI GAWTC 8 cut(s) 227, 326, 640, 796, 960, 1231, 1322, 1614
PfoI TCCNGGA 2 cut(s) 883, 1458
PkrI GCNGC 4 cut(s) 321, 1102, 1141, 1535
PleI GAGTC 4 cut(s) 446, 525, 1021, 1061
PpsI GAGTC 4 cut(s) 446, 525, 1021, 1061
Ppu21I YACGTR 1 cut(s) 1005
PscI ACATGT 1 cut(s) 1011
Psp6I CCWGG 3 cut(s) 280, 883, 1221
PspFI CCCAGC 1 cut(s) 1347
PspGI CCWGG 3 cut(s) 280, 883, 1221
PspN4I GGNNCC 2 cut(s) 1389, 1457
PstNI CAGNNNCTG 2 cut(s) 230, 281
PsuI RGATCY 1 cut(s) 592
PvuII CAGCTG 1 cut(s) 322
RsaI GTAC 4 cut(s) 279, 1238, 1389, 1651
RsaNI GTAC 4 cut(s) 278, 1237, 1388, 1650
RseI CAYNNNNRTG 2 cut(s) 189, 719
SapI GCTCTTC 1 cut(s) 1191
SaqAI TTAA 3 cut(s) 698, 708, 1335
SatI GCNGC 4 cut(s) 320, 1101, 1140, 1534
Sau3AI GATC 8 cut(s) 272, 389, 437, 484, 592, 627, 820, 940
SchI GAGTC 4 cut(s) 446, 525, 1021, 1061
ScrFI CCNGG 4 cut(s) 282, 885, 1223, 1460
SduI GDGCHC 2 cut(s) 883, 1288
SfaNI GCATC 3 cut(s) 629, 796, 818
SfcI CTRYAG 1 cut(s) 557
SmiMI CAYNNNNRTG 2 cut(s) 189, 719
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
Sse9I AATT 5 cut(s) 405, 906, 1158, 1507, 1633
SsiI CCGC 3 cut(s) 60, 861, 1101
SspMI CTAG 6 cut(s) 459, 938, 995, 1097, 1208, 1530
StyD4I CCNGG 4 cut(s) 280, 883, 1221, 1458
StyI CCWWGG 1 cut(s) 1104
TaaI ACNGT 3 cut(s) 718, 855, 1297
TaiI ACGT 3 cut(s) 56, 876, 1007
TaqI TCGA 6 cut(s) 197, 440, 589, 785, 1261, 1555
TasI AATT 5 cut(s) 405, 906, 1158, 1507, 1633
TauI GCSGC 1 cut(s) 1103
TfiI GAWTC 8 cut(s) 227, 326, 640, 796, 960, 1231, 1322, 1614
Tru1I TTAA 3 cut(s) 698, 708, 1335
Tru9I TTAA 3 cut(s) 698, 708, 1335
TscAI CASTG 4 cut(s) 315, 348, 691, 721
TseI GCWGC 3 cut(s) 319, 1139, 1533
TspGWI ACGGA 1 cut(s) 1052
TspRI CASTG 4 cut(s) 315, 348, 691, 721
XapI RAATTY 1 cut(s) 405
XceI RCATGY 1 cut(s) 1015
XmiI GTMKAC 2 cut(s) 208, 1065
XmnI GAANNNNTTC 4 cut(s) 383, 759, 1370, 1603
XspI CTAG 6 cut(s) 459, 938, 995, 1097, 1208, 1530
Zsp2I ATGCAT 1 cut(s) 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.