Rh4DG303500

65-kDa microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007124
Physical Location & Seq
Reverse (-)
52537302 .. 52539407
2106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG303500.1

Sequence Viewer

Length: 1137 bp
ATGGGTTCATTTCGAGCACCAGTGGGGAGAAGCTCTGCGCTTCCTGAAACATCATGTGGATATTTGCTTCAAGAACTACAGATGATTTGGAATGAAATTGGACAAGATCAGTTGGAAAGGGAGAAGGTACTGGTGGATTTAGAGCAGGAATGCCTGGAGGTCTATAGGAGGAAGGTTGATGCTGCTAATACCTCAAGAGCTCGCCTGCACCAGGAGTTGGCAGAAGCTGAGGCTGAATTTACGCATCTTCTTTTGTCACTTGGCGAACGGTCTCTCCCCGGACGGCCAGAAAAAATGGTGGGAACACTGAAAGAGCAGCTAGATTCGATCACGCCAGCTCTGCGAGACATGAGATTGAGGAAAGAAGATAGGGTGAACAAGTTCCGAGCTGTACAAGGGCAAATTCAGAAGATCTCTGCAGAAATAGCAGGACAGTCAGAGTATGAGAACTCATCATCAGATGTCACGGTGAATGAGAATGATCTTTCCTTGAAGAAACTAGAGGAGTACCAGACGGAGCTACAAAGACTCCACAATGACAGGAATGAAAGGCTCAAGCGGGTGGAGAAATATATAAACTCCATCCAAAAGTTGTCTTCAATATTAGGAACAGACTCCTCTATGGTCATCACCAAGGTTCACCCAAGCTTGAATGAGTTATGTGGGATAGCCAAGAACATAAGCAACAGTATTTTGGCTAAACTCAACAGCACAGTGGAATCCCTAGAGGAAGAAAAGCAAAAGCGGCTTGAAAAGCTTCACCATCTTGGTAAAGCATTGACAAACTTATGGAATCTCATGGATACACCATATAAAGATCGTCAATTGTTTTCCCATGTTACCGGCTTATTATCAGTCTCATCAGCAGAAGTATCAGATCCAGGAAGCCTAACCCAAAAGATAATCCAGCAGGCTGAGGCTGAAGTCAAGAGACTGGATCAATTAAAAGTAAGCAAGATGAAAGAACTATTTCTCAAGAAACAAAATGAGCTCAAGGAGATATGCAAAAAATCACACATGGAGATTCCTGTGCAAGCAAAGATGGATAATATAATTAACCTCATGAAATCTGGTGAGATTCATCAATATGTGCTTACTCTAAGAATGAAGACATGTCATTGCTTAAAGATGTTGTAG

Protein Analysis

378

Amino Acids

43.19

Weight (kDa)

8.09

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 22 - 341 5.1e-44 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 217
AciI CCGC 2 cut(s) 559, 745
AclWI GGATC 2 cut(s) 872, 945
AcoI YGGCCR 1 cut(s) 284
AcsI RAATTY 2 cut(s) 236, 402
AcuI CTGAAG 1 cut(s) 942
AfaI GTAC 3 cut(s) 129, 393, 509
AfiI CCNNNNNNNGG 2 cut(s) 211, 217
AflIII ACRYGT 1 cut(s) 1112
AgsI TTSAA 5 cut(s) 71, 493, 600, 652, 752
AjnI CCWGG 3 cut(s) 153, 210, 880
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
Alw21I GWGCWC 3 cut(s) 19, 202, 993
Alw26I GTCTC 4 cut(s) 276, 339, 862, 925
AlwI GGATC 2 cut(s) 872, 945
AlwNI CAGNNNCTG 1 cut(s) 227
AoxI GGCC 1 cut(s) 284
ApeKI GCWGC 2 cut(s) 182, 316
ApoI RAATTY 2 cut(s) 236, 402
Asp700I GAANNNNTTC 3 cut(s) 380, 756, 969
AspLEI GCGC 1 cut(s) 40
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 6 cut(s) 385, 481, 622, 632, 752, 1085
BanII GRGCYC 2 cut(s) 202, 993
BbsI GAAGAC 2 cut(s) 588, 1115
Bbv12I GWGCWC 3 cut(s) 19, 202, 993
BbvCI CCTCAGC 2 cut(s) 228, 915
BbvI GCAGC 2 cut(s) 169, 328
BccI CCATC 3 cut(s) 590, 771, 1036
BceAI ACGGC 1 cut(s) 299
BciT130I CCWGG 3 cut(s) 155, 212, 882
BciVI GTATCC 1 cut(s) 796
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 4 cut(s) 276, 339, 862, 925
BfaI CTAG 3 cut(s) 320, 500, 725
BfmI CTRYAG 3 cut(s) 77, 163, 417
BfuI GTATCC 1 cut(s) 796
BglII AGATCT 1 cut(s) 411
BisI GCNGC 3 cut(s) 183, 317, 746
BlsI GCNGC 3 cut(s) 184, 318, 747
Bme1390I CCNGG 4 cut(s) 155, 212, 279, 882
BmrFI CCNGG 4 cut(s) 155, 212, 279, 882
BmsI GCATC 2 cut(s) 169, 253
BpiI GAAGAC 2 cut(s) 588, 1115
BpmI CTGGAG 1 cut(s) 176
Bpu10I CCTNAGC 2 cut(s) 228, 915
BpuEI CTTGAG 4 cut(s) 178, 539, 959, 977
BpuMI CCSGG 1 cut(s) 279
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 2 cut(s) 277, 633
BsaXI ACNNNNNCTCC 4 cut(s) 258, 288, 513, 543
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 217
Bse118I RCCGGY 1 cut(s) 842
Bse1I ACTGG 3 cut(s) 20, 135, 939
Bse3DI GCAATG 1 cut(s) 1117
BseBI CCWGG 3 cut(s) 155, 212, 882
BseDI CCNNGG 2 cut(s) 277, 633
BseGI GGATG 1 cut(s) 582
BseLI CCNNNNNNNGG 2 cut(s) 211, 217
BseMI GCAATG 1 cut(s) 1117
BseMII CTCAG 2 cut(s) 219, 906
BseNI ACTGG 3 cut(s) 20, 135, 939
BseRI GAGGAG 2 cut(s) 518, 607
BseXI GCAGC 2 cut(s) 169, 328
BsgI GTGCAG 1 cut(s) 191
BshFI GGCC 1 cut(s) 286
BsiHKAI GWGCWC 3 cut(s) 19, 202, 993
BsiSI CCGG 2 cut(s) 279, 843
BslI CCNNNNNNNGG 2 cut(s) 211, 217
BsmAI GTCTC 4 cut(s) 276, 339, 862, 925
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 1 cut(s) 286
Bso31I GGTCTC 1 cut(s) 276
Bsp1286I GDGCHC 3 cut(s) 19, 202, 993
Bsp1407I TGTACA 1 cut(s) 391
Bsp143I GATC 7 cut(s) 106, 327, 411, 481, 817, 877, 937
BspACI CCGC 2 cut(s) 559, 745
BspANI GGCC 1 cut(s) 286
BspCNI CTCAG 2 cut(s) 220, 907
BspHI TCATGA 1 cut(s) 1062
BspMAI CTGCAG 1 cut(s) 421
BspPI GGATC 2 cut(s) 872, 945
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 1117
BsrFI RCCGGY 1 cut(s) 842
BsrGI TGTACA 1 cut(s) 391
BsrI ACTGG 3 cut(s) 20, 135, 939
BssAI RCCGGY 1 cut(s) 842
BssECI CCNNGG 2 cut(s) 277, 633
BssMI GATC 7 cut(s) 106, 327, 411, 481, 817, 877, 937
BssT1I CCWWGG 1 cut(s) 633
Bst2UI CCWGG 3 cut(s) 155, 212, 882
Bst4CI ACNGT 5 cut(s) 270, 435, 469, 689, 715
BstAUI TGTACA 1 cut(s) 391
BstC8I GCNNGC 5 cut(s) 202, 206, 336, 912, 1035
BstDEI CTNAG 3 cut(s) 228, 915, 1100
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 1 cut(s) 582
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 7 cut(s) 109, 330, 414, 484, 820, 880, 940
BstMAI GTCTC 4 cut(s) 276, 339, 862, 925
BstMBI GATC 7 cut(s) 106, 327, 411, 481, 817, 877, 937
BstMWI GCNNNNNNNGC 4 cut(s) 340, 425, 745, 754
BstNI CCWGG 3 cut(s) 155, 212, 882
BstNSI RCATGY 1 cut(s) 1116
BstSCI CCNGG 4 cut(s) 153, 210, 277, 880
BstSFI CTRYAG 3 cut(s) 77, 163, 417
BstV1I GCAGC 2 cut(s) 169, 328
BstV2I GAAGAC 2 cut(s) 588, 1115
BstX2I RGATCY 2 cut(s) 411, 877
BstYI RGATCY 2 cut(s) 411, 877
BsuI GTATCC 1 cut(s) 796
BsuRI GGCC 1 cut(s) 286
BtsCI GGATG 1 cut(s) 582
BtsIMutI CAGTG 3 cut(s) 27, 305, 720
Cac8I GCNNGC 5 cut(s) 202, 206, 336, 912, 1035
CaiI CAGNNNCTG 1 cut(s) 227
CciI TCATGA 1 cut(s) 1062
CfoI GCGC 1 cut(s) 40
Cfr10I RCCGGY 1 cut(s) 842
Csp6I GTAC 3 cut(s) 128, 392, 508
CviAII CATG 7 cut(s) 54, 349, 799, 836, 1018, 1063, 1113
CviQI GTAC 3 cut(s) 128, 392, 508
DdeI CTNAG 3 cut(s) 228, 915, 1100
DpnI GATC 7 cut(s) 108, 329, 413, 483, 819, 879, 939
DpnII GATC 7 cut(s) 106, 327, 411, 481, 817, 877, 937
EaeI YGGCCR 1 cut(s) 284
Ecl136II GAGCTC 2 cut(s) 200, 991
Eco130I CCWWGG 1 cut(s) 633
Eco24I GRGCYC 2 cut(s) 202, 993
Eco31I GGTCTC 1 cut(s) 276
Eco53kI GAGCTC 2 cut(s) 200, 991
Eco57I CTGAAG 1 cut(s) 942
EcoICRI GAGCTC 2 cut(s) 200, 991
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoRII CCWGG 3 cut(s) 153, 210, 880
EcoT14I CCWWGG 1 cut(s) 633
EcoT38I GRGCYC 2 cut(s) 202, 993
ErhI CCWWGG 1 cut(s) 633
FaeI CATG 7 cut(s) 57, 352, 802, 839, 1021, 1066, 1116
FatI CATG 7 cut(s) 53, 348, 798, 835, 1017, 1062, 1112
FauI CCCGC 1 cut(s) 552
Fnu4HI GCNGC 3 cut(s) 183, 317, 746
FokI GGATG 1 cut(s) 569
FriOI GRGCYC 2 cut(s) 202, 993
Fsp4HI GCNGC 3 cut(s) 183, 317, 746
FspBI CTAG 3 cut(s) 320, 500, 725
GlaI GCGC 1 cut(s) 39
GluI GCNGC 3 cut(s) 183, 317, 746
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 1 cut(s) 286
HapII CCGG 2 cut(s) 279, 843
HhaI GCGC 1 cut(s) 40
Hin1II CATG 7 cut(s) 57, 352, 802, 839, 1021, 1066, 1116
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HindIII AAGCTT 2 cut(s) 646, 755
HinfI GANTC 7 cut(s) 323, 528, 614, 719, 793, 1024, 1078
HpaII CCGG 2 cut(s) 279, 843
HphI GGTGA 6 cut(s) 385, 481, 622, 632, 752, 1085
Hpy166II GTNNAC 2 cut(s) 376, 640
Hpy188I TCNGA 5 cut(s) 386, 408, 439, 460, 877
Hpy188III TCNNGA 6 cut(s) 44, 71, 195, 928, 976, 1063
Hpy8I GTNNAC 2 cut(s) 376, 640
HpyAV CCTTC 2 cut(s) 118, 166
HpyCH4III ACNGT 5 cut(s) 270, 435, 469, 689, 715
HpyCH4V TGCA 4 cut(s) 208, 419, 1005, 1033
HpyF10VI GCNNNNNNNGC 4 cut(s) 340, 425, 745, 754
HpyF3I CTNAG 3 cut(s) 228, 915, 1100
Hsp92II CATG 7 cut(s) 57, 352, 802, 839, 1021, 1066, 1116
HspAI GCGC 1 cut(s) 38
Kzo9I GATC 7 cut(s) 106, 327, 411, 481, 817, 877, 937
LmnI GCTCC 1 cut(s) 517
Lsp1109I GCAGC 2 cut(s) 169, 328
LweI GCATC 2 cut(s) 169, 253
MaeI CTAG 3 cut(s) 320, 500, 725
MaeIII GTNAC 3 cut(s) 255, 463, 838
MalI GATC 7 cut(s) 108, 329, 413, 483, 819, 879, 939
MboI GATC 7 cut(s) 106, 327, 411, 481, 817, 877, 937
MboII GAAGA 7 cut(s) 239, 377, 421, 505, 588, 743, 1120
MfeI CAATTG 1 cut(s) 824
MflI RGATCY 2 cut(s) 411, 877
MhlI GDGCHC 3 cut(s) 19, 202, 993
MluCI AATT 6 cut(s) 96, 236, 402, 824, 941, 1053
MlyI GAGTC 2 cut(s) 522, 608
MmeI TCCRAC 1 cut(s) 93
MroXI GAANNNNTTC 3 cut(s) 380, 756, 969
MseI TTAA 3 cut(s) 944, 1056, 1124
MslI CAYNNNNRTG 1 cut(s) 1086
MspI CCGG 2 cut(s) 279, 843
MspR9I CCNGG 4 cut(s) 155, 212, 279, 882
MunI CAATTG 1 cut(s) 824
Mva1269I GAATGC 1 cut(s) 155
MvaI CCWGG 3 cut(s) 155, 212, 882
MwoI GCNNNNNNNGC 4 cut(s) 340, 425, 745, 754
NciI CCSGG 1 cut(s) 279
NdeII GATC 7 cut(s) 106, 327, 411, 481, 817, 877, 937
NlaIII CATG 7 cut(s) 57, 352, 802, 839, 1021, 1066, 1116
NmuCI GTSAC 2 cut(s) 255, 463
NspI RCATGY 1 cut(s) 1116
PagI TCATGA 1 cut(s) 1062
PciI ACATGT 1 cut(s) 1112
PctI GAATGC 1 cut(s) 155
PdmI GAANNNNTTC 3 cut(s) 380, 756, 969
PfeI GAWTC 5 cut(s) 323, 719, 793, 1024, 1078
PflMI CCANNNNNTGG 1 cut(s) 217
PfoI TCCNGGA 1 cut(s) 880
PkrI GCNGC 3 cut(s) 184, 318, 747
PleI GAGTC 2 cut(s) 522, 608
PpsI GAGTC 2 cut(s) 522, 608
PscI ACATGT 1 cut(s) 1112
Psp124BI GAGCTC 2 cut(s) 202, 993
Psp6I CCWGG 3 cut(s) 153, 210, 880
PspGI CCWGG 3 cut(s) 153, 210, 880
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 227
PsuI RGATCY 2 cut(s) 411, 877
RsaI GTAC 3 cut(s) 129, 393, 509
RsaNI GTAC 3 cut(s) 128, 392, 508
RseI CAYNNNNRTG 1 cut(s) 1086
SacI GAGCTC 2 cut(s) 202, 993
SaqAI TTAA 3 cut(s) 944, 1056, 1124
SatI GCNGC 3 cut(s) 183, 317, 746
Sau3AI GATC 7 cut(s) 106, 327, 411, 481, 817, 877, 937
SchI GAGTC 2 cut(s) 522, 608
ScrFI CCNGG 4 cut(s) 155, 212, 279, 882
SduI GDGCHC 3 cut(s) 19, 202, 993
SfaNI GCATC 2 cut(s) 169, 253
SfcI CTRYAG 3 cut(s) 77, 163, 417
SmiMI CAYNNNNRTG 1 cut(s) 1086
SmlI CTYRAG 4 cut(s) 193, 554, 974, 992
SmoI CTYRAG 4 cut(s) 193, 554, 974, 992
Sse9I AATT 6 cut(s) 96, 236, 402, 824, 941, 1053
SsiI CCGC 2 cut(s) 559, 745
SspI AATATT 1 cut(s) 603
SspMI CTAG 3 cut(s) 320, 500, 725
SstI GAGCTC 2 cut(s) 202, 993
StyD4I CCNGG 4 cut(s) 153, 210, 277, 880
StyI CCWWGG 1 cut(s) 633
TaaI ACNGT 5 cut(s) 270, 435, 469, 689, 715
TaqI TCGA 2 cut(s) 13, 326
TasI AATT 6 cut(s) 96, 236, 402, 824, 941, 1053
TatI WGTACW 1 cut(s) 391
TauI GCSGC 1 cut(s) 748
TfiI GAWTC 5 cut(s) 323, 719, 793, 1024, 1078
Tru1I TTAA 3 cut(s) 944, 1056, 1124
Tru9I TTAA 3 cut(s) 944, 1056, 1124
TscAI CASTG 3 cut(s) 27, 312, 720
TseFI GTSAC 2 cut(s) 255, 463
TseI GCWGC 2 cut(s) 182, 316
Tsp45I GTSAC 2 cut(s) 255, 463
TspDTI ATGAA 6 cut(s) 108, 561, 974, 1070, 1079, 1121
TspGWI ACGGA 1 cut(s) 530
TspRI CASTG 3 cut(s) 27, 312, 720
Van91I CCANNNNNTGG 1 cut(s) 217
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 2 cut(s) 236, 402
XceI RCATGY 1 cut(s) 1116
XmnI GAANNNNTTC 3 cut(s) 380, 756, 969
XspI CTAG 3 cut(s) 320, 500, 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.