Rw4G025990

65-kDa microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0007124
Physical Location & Seq
Reverse (-)
52125668 .. 52129864
4197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025990.1

Sequence Viewer

Length: 1734 bp
ATGGGTTCATTTCGAGCACCAGTGGGGAGAAGCTCTGCGCTTCCTGAAACATCATGTGGATATTTGCTTCAAGAACTACAGATGATTTGGAATGAAATTGGACAAGATCAGTTGGAAAGGGAGAAGGTACTGGTGGATTTAGAGCAGGAATGCCTGGAGGTCTATAGGAGGAAGGTTGATGCTGCTAATACCTCAAGAGCTCGCCTGCACCAGGAGTTGGCAGAAGCTGAGGCTGAATTTACGCATCTTCTTTTGTCACTTGGCGAACGGTCTCTCCCCGGACGGCCAGAAAAAATGGTGGGAACACTGAAAGAGCAGCTAGATTCGATCACGCCAGCTCTGCAAGACATGAGATTGAGGAAAGAAGATAGGGTGAACAAGTTCCGAGCTTCAGAGTATGAGAACTCATCATCAGATGTCAGGGTGAATGAGAATGATCTTTCCTTGAAGAAACTAGAGGAGTACCAGACGGAGCTACAAAGACTCCACAATGACAGGAATGAAAGGCTCAAGCGGGTGGAGAAATATATAAACTCCATCCAAAAGTTGTCTTCAATATTAGGAACAGACTCCTCTATGGTCATCACCAAGGTTCACCCAAGCTTGAATGAGTTATGTGGGATAGCCAAGAACATAAGCAACAGTATTTTGGCTAAACTCAACAGCACAGTGGAATCCCTAGAGGAAGAAAAGCAAAAGCGGCTTGGAAAGCTTCACCATCTTGGTAAAGCATTGACAAACTTATGGAATCTCATGGATACACCATATAAAGATCGTCAATTGTTTTCCCATGTTACCGGCTTATTATCAGTCTCATCAGCAGAAGTATCAGATCCAGGAAGCCTAACCCAAAAGATAATCCAGCAGGCTGAGGCTGAAGTCAAGAGACTGGATCAATTAAAAGTAAGCAAGATGAAAGAACTATTTCTCAAGAAACAAAATGAGCTCAAGGAGATATGCAAAAAATCACACATGGAGATTCCTTTGCAAGCAAAGATGGATAATATAATTAACCTCATGAAATCTGGGGAAATTGACCATGCAGATCTTGTCATGAGCATGGATCAACAGATATCAAGAGCAGAAGATGAAGCTTGTAGCAGAAAGACAATTATGGAAAAGGTGGAAAAGTGGATCCTAGCGCGTGATGAGGAGCGCTGGTTGGAAGAATATGGAAGAGACGAAAACCGATACTCAGTCAGAAGAGGTGCACACAAGAACCTGAGACGTGCAGAACGTGCTCGAGTATTAGTTAGCAAAATTCCAGCTTTGGTGGATTTACTGATAGAAAACACTAGGAGTTGGGAAGAAGAAAGAAAGAAGACTTTCTTATATGATGAGGTACCTCTCCTAGCAATGTTGGAAGAGTATATTGTGTTGAGGCAGGAAAAGGAGGGGGAGAGGCAAAGACAAAGGGAAGTGAAGTTCCAAGGCCTAATAGGACAACATCAGCCAGAAAATTTGTTCCTGCCCAGTCCAAGCAGCAGTCCTAGGCGAATTTCAAACAGAAGTGTAAATGGAGGCTTTAGCACTGCTATGCCTCTAAACAGAAGACTTTCTCTGGGCCTTCAGAAACTGGGACCAAACAGCATAGATTTAGGCACTCAAGGCATATCAAAGGAAGGTAAGAAGGCACAGAGACAAAAGATGTTTGCAAGACCTGGTCTTGTTTCTCCTGCTAGAGATGAAACAGCTTCAGTGGTATCAACATTTTCTGGCCCACTGTCTCCCTGA

Protein Analysis

577

Amino Acids

65.99

Weight (kDa)

8.78

Isoelectric Point (pI)

55.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 22 - 474 6.4e-66 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1342
AccB1I GGYRCC 1 cut(s) 1342
AccB7I CCANNNNNTGG 1 cut(s) 217
AccII CGCG 1 cut(s) 1144
AciI CCGC 2 cut(s) 514, 700
AclWI GGATC 5 cut(s) 827, 900, 1071, 1129, 1142
AcoI YGGCCR 1 cut(s) 284
AcsI RAATTY 4 cut(s) 236, 1260, 1459, 1497
AcuI CTGAAG 4 cut(s) 375, 897, 1553, 1680
AfaI GTAC 3 cut(s) 129, 464, 1344
AfeI AGCGCT 1 cut(s) 1157
AfiI CCNNNNNNNGG 2 cut(s) 211, 217
AgsI TTSAA 5 cut(s) 71, 448, 555, 607, 1503
AjiI CACGTC 1 cut(s) 1229
AjnI CCWGG 4 cut(s) 153, 210, 835, 1660
AloI GAACNNNNNNTCC 4 cut(s) 258, 290, 1409, 1441
Alw21I GWGCWC 5 cut(s) 19, 202, 948, 1213, 1243
Alw26I GTCTC 6 cut(s) 276, 817, 880, 1173, 1219, 1633
Alw44I GTGCAC 1 cut(s) 1209
AlwI GGATC 5 cut(s) 827, 900, 1071, 1129, 1142
AlwNI CAGNNNCTG 2 cut(s) 227, 1576
Ama87I CYCGRG 1 cut(s) 1242
Aor51HI AGCGCT 1 cut(s) 1157
AoxI GGCC 4 cut(s) 284, 1432, 1564, 1717
ApaLI GTGCAC 1 cut(s) 1209
ApeKI GCWGC 3 cut(s) 182, 316, 1482
ApoI RAATTY 4 cut(s) 236, 1260, 1459, 1497
Asp700I GAANNNNTTC 4 cut(s) 380, 924, 1325, 1555
Asp718I GGTACC 1 cut(s) 1342
AspA2I CCTAGG 1 cut(s) 1490
AspLEI GCGC 3 cut(s) 40, 1144, 1158
AspS9I GGNCC 3 cut(s) 1564, 1580, 1718
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 5 cut(s) 385, 436, 577, 587, 707
AvaI CYCGRG 1 cut(s) 1242
AvaII GGWCC 1 cut(s) 1580
AvrII CCTAGG 1 cut(s) 1490
BaeGI GKGCMC 1 cut(s) 1213
BamHI GGATCC 1 cut(s) 1134
BanI GGYRCC 1 cut(s) 1342
BanII GRGCYC 2 cut(s) 202, 948
BbsI GAAGAC 3 cut(s) 543, 1328, 1558
Bbv12I GWGCWC 5 cut(s) 19, 202, 948, 1213, 1243
BbvCI CCTCAGC 2 cut(s) 228, 870
BbvI GCAGC 3 cut(s) 169, 328, 1494
BccI CCATC 3 cut(s) 545, 726, 991
BceAI ACGGC 1 cut(s) 299
BciT130I CCWGG 4 cut(s) 155, 212, 837, 1662
BciVI GTATCC 1 cut(s) 751
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 6 cut(s) 276, 817, 880, 1173, 1219, 1633
BfaI CTAG 8 cut(s) 320, 455, 680, 1139, 1296, 1352, 1491, 1680
BfmI CTRYAG 2 cut(s) 77, 163
BfoI RGCGCY 1 cut(s) 1159
BfuI GTATCC 1 cut(s) 751
BglII AGATCT 1 cut(s) 1045
BisI GCNGC 4 cut(s) 183, 317, 701, 1483
BlnI CCTAGG 1 cut(s) 1490
BlsI GCNGC 4 cut(s) 184, 318, 702, 1484
Bme1390I CCNGG 5 cut(s) 155, 212, 279, 837, 1662
Bme18I GGWCC 1 cut(s) 1580
BmeT110I CYCGRG 1 cut(s) 1242
BmgBI CACGTC 1 cut(s) 1229
BmgT120I GGNCC 3 cut(s) 1564, 1580, 1718
BmiI GGNNCC 3 cut(s) 1136, 1344, 1581
BmrFI CCNGG 5 cut(s) 155, 212, 279, 837, 1662
BmrI ACTGGG 2 cut(s) 1467, 1586
BmsI GCATC 2 cut(s) 169, 253
BmuI ACTGGG 2 cut(s) 1467, 1586
BpiI GAAGAC 3 cut(s) 543, 1328, 1558
BpmI CTGGAG 1 cut(s) 176
Bpu10I CCTNAGC 2 cut(s) 228, 870
BpuEI CTTGAG 5 cut(s) 178, 494, 914, 932, 1590
BpuMI CCSGG 1 cut(s) 279
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 4 cut(s) 277, 588, 1429, 1490
BsaXI ACNNNNNCTCC 6 cut(s) 258, 288, 468, 498, 1145, 1175
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 217
Bse118I RCCGGY 1 cut(s) 797
Bse1I ACTGG 5 cut(s) 20, 135, 894, 1473, 1581
Bse3DI GCAATG 1 cut(s) 1362
BseBI CCWGG 4 cut(s) 155, 212, 837, 1662
BseDI CCNNGG 4 cut(s) 277, 588, 1429, 1490
BseGI GGATG 1 cut(s) 537
BseLI CCNNNNNNNGG 2 cut(s) 211, 217
BseMI GCAATG 1 cut(s) 1362
BseMII CTCAG 4 cut(s) 219, 861, 1209, 1214
BseNI ACTGG 5 cut(s) 20, 135, 894, 1473, 1581
BseRI GAGGAG 3 cut(s) 473, 562, 1166
BseSI GKGCMC 1 cut(s) 1213
BseXI GCAGC 3 cut(s) 169, 328, 1494
BsgI GTGCAG 2 cut(s) 191, 1251
Bsh1236I CGCG 1 cut(s) 1144
BshFI GGCC 4 cut(s) 286, 1434, 1566, 1719
BshNI GGYRCC 1 cut(s) 1342
BsiHKAI GWGCWC 5 cut(s) 19, 202, 948, 1213, 1243
BsiHKCI CYCGRG 1 cut(s) 1242
BsiSI CCGG 2 cut(s) 279, 798
BslFI GGGAC 1 cut(s) 1593
BslI CCNNNNNNNGG 2 cut(s) 211, 217
BsmAI GTCTC 6 cut(s) 276, 817, 880, 1173, 1219, 1633
BsmBI CGTCTC 2 cut(s) 1173, 1219
BsmFI GGGAC 1 cut(s) 1593
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 4 cut(s) 286, 1434, 1566, 1719
Bso31I GGTCTC 1 cut(s) 276
BsoBI CYCGRG 1 cut(s) 1242
Bsp1286I GDGCHC 5 cut(s) 19, 202, 948, 1213, 1243
Bsp143I GATC 9 cut(s) 106, 327, 436, 772, 832, 892, 1045, 1063, 1134
BspACI CCGC 2 cut(s) 514, 700
BspANI GGCC 4 cut(s) 286, 1434, 1566, 1719
BspCNI CTCAG 4 cut(s) 220, 862, 1208, 1215
BspFNI CGCG 1 cut(s) 1144
BspHI TCATGA 2 cut(s) 1017, 1053
BspLI GGNNCC 3 cut(s) 1136, 1344, 1581
BspPI GGATC 5 cut(s) 827, 900, 1071, 1129, 1142
BspT107I GGYRCC 1 cut(s) 1342
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 1362
BsrFI RCCGGY 1 cut(s) 797
BsrI ACTGG 5 cut(s) 20, 135, 894, 1473, 1581
BssAI RCCGGY 1 cut(s) 797
BssECI CCNNGG 4 cut(s) 277, 588, 1429, 1490
BssMI GATC 9 cut(s) 106, 327, 436, 772, 832, 892, 1045, 1063, 1134
BssT1I CCWWGG 3 cut(s) 588, 1429, 1490
Bst2UI CCWGG 4 cut(s) 155, 212, 837, 1662
Bst4CI ACNGT 4 cut(s) 270, 644, 670, 1725
Bst6I CTCTTC 3 cut(s) 1171, 1198, 1359
BstAPI GCANNNNNTGC 1 cut(s) 1238
BstC8I GCNNGC 5 cut(s) 202, 206, 336, 867, 990
BstDEI CTNAG 4 cut(s) 228, 870, 1195, 1223
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 1 cut(s) 537
BstFNI CGCG 1 cut(s) 1144
BstH2I RGCGCY 1 cut(s) 1159
BstHHI GCGC 3 cut(s) 40, 1144, 1158
BstKTI GATC 9 cut(s) 109, 330, 439, 775, 835, 895, 1048, 1066, 1137
BstMAI GTCTC 6 cut(s) 276, 817, 880, 1173, 1219, 1633
BstMBI GATC 9 cut(s) 106, 327, 436, 772, 832, 892, 1045, 1063, 1134
BstMWI GCNNNNNNNGC 5 cut(s) 340, 700, 709, 1238, 1608
BstNI CCWGG 4 cut(s) 155, 212, 837, 1662
BstSCI CCNGG 5 cut(s) 153, 210, 277, 835, 1660
BstSFI CTRYAG 2 cut(s) 77, 163
BstSLI GKGCMC 1 cut(s) 1213
BstUI CGCG 1 cut(s) 1144
BstV1I GCAGC 3 cut(s) 169, 328, 1494
BstV2I GAAGAC 3 cut(s) 543, 1328, 1558
BstX2I RGATCY 3 cut(s) 832, 1045, 1134
BstYI RGATCY 3 cut(s) 832, 1045, 1134
BsuI GTATCC 1 cut(s) 751
BsuRI GGCC 4 cut(s) 286, 1434, 1566, 1719
BtrI CACGTC 1 cut(s) 1229
BtsCI GGATG 1 cut(s) 537
BtsI GCAGTG 1 cut(s) 1530
BtsIMutI CAGTG 6 cut(s) 27, 305, 675, 1530, 1704, 1721
Cac8I GCNNGC 5 cut(s) 202, 206, 336, 867, 990
CaiI CAGNNNCTG 2 cut(s) 227, 1576
CciI TCATGA 2 cut(s) 1017, 1053
CfoI GCGC 3 cut(s) 40, 1144, 1158
Cfr10I RCCGGY 1 cut(s) 797
Cfr13I GGNCC 3 cut(s) 1564, 1580, 1718
CsiI ACCWGGT 1 cut(s) 1660
Csp6I GTAC 3 cut(s) 128, 463, 1343
CviAII CATG 9 cut(s) 54, 349, 754, 791, 973, 1018, 1040, 1054, 1060
CviQI GTAC 3 cut(s) 128, 463, 1343
DdeI CTNAG 4 cut(s) 228, 870, 1195, 1223
DpnI GATC 9 cut(s) 108, 329, 438, 774, 834, 894, 1047, 1065, 1136
DpnII GATC 9 cut(s) 106, 327, 436, 772, 832, 892, 1045, 1063, 1134
EaeI YGGCCR 1 cut(s) 284
Eam1104I CTCTTC 3 cut(s) 1171, 1198, 1359
EarI CTCTTC 3 cut(s) 1171, 1198, 1359
Ecl136II GAGCTC 2 cut(s) 200, 946
Eco130I CCWWGG 3 cut(s) 588, 1429, 1490
Eco147I AGGCCT 1 cut(s) 1434
Eco24I GRGCYC 2 cut(s) 202, 948
Eco31I GGTCTC 1 cut(s) 276
Eco32I GATATC 1 cut(s) 1074
Eco47I GGWCC 1 cut(s) 1580
Eco47III AGCGCT 1 cut(s) 1157
Eco53kI GAGCTC 2 cut(s) 200, 946
Eco57I CTGAAG 4 cut(s) 375, 897, 1553, 1680
Eco88I CYCGRG 1 cut(s) 1242
EcoICRI GAGCTC 2 cut(s) 200, 946
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoRII CCWGG 4 cut(s) 153, 210, 835, 1660
EcoRV GATATC 1 cut(s) 1074
EcoT14I CCWWGG 3 cut(s) 588, 1429, 1490
EcoT38I GRGCYC 2 cut(s) 202, 948
ErhI CCWWGG 3 cut(s) 588, 1429, 1490
Esp3I CGTCTC 2 cut(s) 1173, 1219
FaeI CATG 9 cut(s) 57, 352, 757, 794, 976, 1021, 1043, 1057, 1063
FalI AAGNNNNNCTT 2 cut(s) 1313, 1345
FaqI GGGAC 1 cut(s) 1593
FatI CATG 9 cut(s) 53, 348, 753, 790, 972, 1017, 1039, 1053, 1059
FauI CCCGC 1 cut(s) 507
Fnu4HI GCNGC 4 cut(s) 183, 317, 701, 1483
FokI GGATG 1 cut(s) 524
FriOI GRGCYC 2 cut(s) 202, 948
Fsp4HI GCNGC 4 cut(s) 183, 317, 701, 1483
FspBI CTAG 8 cut(s) 320, 455, 680, 1139, 1296, 1352, 1491, 1680
GlaI GCGC 3 cut(s) 39, 1143, 1157
GluI GCNGC 4 cut(s) 183, 317, 701, 1483
GsuI CTGGAG 1 cut(s) 176
HaeII RGCGCY 1 cut(s) 1159
HaeIII GGCC 4 cut(s) 286, 1434, 1566, 1719
HapII CCGG 2 cut(s) 279, 798
HhaI GCGC 3 cut(s) 40, 1144, 1158
Hin1II CATG 9 cut(s) 57, 352, 757, 794, 976, 1021, 1043, 1057, 1063
Hin6I GCGC 3 cut(s) 38, 1142, 1156
HinP1I GCGC 3 cut(s) 38, 1142, 1156
HindIII AAGCTT 3 cut(s) 601, 710, 1092
HinfI GANTC 6 cut(s) 323, 483, 569, 674, 748, 979
HpaII CCGG 2 cut(s) 279, 798
HphI GGTGA 5 cut(s) 385, 436, 577, 587, 707
Hpy166II GTNNAC 3 cut(s) 376, 595, 1211
Hpy188I TCNGA 6 cut(s) 386, 394, 415, 832, 1202, 1572
Hpy188III TCNNGA 8 cut(s) 44, 71, 195, 883, 931, 1018, 1054, 1077
Hpy8I GTNNAC 3 cut(s) 376, 595, 1211
HpyAV CCTTC 5 cut(s) 118, 166, 1577, 1616, 1624
HpyCH4III ACNGT 4 cut(s) 270, 644, 670, 1725
HpyCH4IV ACGT 2 cut(s) 1228, 1237
HpyCH4V TGCA 8 cut(s) 208, 343, 960, 988, 1043, 1211, 1232, 1655
HpyF10VI GCNNNNNNNGC 5 cut(s) 340, 700, 709, 1238, 1608
HpyF3I CTNAG 4 cut(s) 228, 870, 1195, 1223
HpySE526I ACGT 2 cut(s) 1228, 1237
Hsp92II CATG 9 cut(s) 57, 352, 757, 794, 976, 1021, 1043, 1057, 1063
HspAI GCGC 3 cut(s) 38, 1142, 1156
KpnI GGTACC 1 cut(s) 1346
Kzo9I GATC 9 cut(s) 106, 327, 436, 772, 832, 892, 1045, 1063, 1134
LmnI GCTCC 2 cut(s) 472, 1153
Lsp1109I GCAGC 3 cut(s) 169, 328, 1494
LweI GCATC 2 cut(s) 169, 253
MabI ACCWGGT 1 cut(s) 1660
MaeI CTAG 8 cut(s) 320, 455, 680, 1139, 1296, 1352, 1491, 1680
MaeII ACGT 2 cut(s) 1228, 1237
MaeIII GTNAC 2 cut(s) 255, 793
MalI GATC 9 cut(s) 108, 329, 438, 774, 834, 894, 1047, 1065, 1136
MboI GATC 9 cut(s) 106, 327, 436, 772, 832, 892, 1045, 1063, 1134
MfeI CAATTG 1 cut(s) 779
MflI RGATCY 3 cut(s) 832, 1045, 1134
MhlI GDGCHC 5 cut(s) 19, 202, 948, 1213, 1243
MlyI GAGTC 2 cut(s) 477, 563
MmeI TCCRAC 3 cut(s) 93, 1143, 1341
MroXI GAANNNNTTC 4 cut(s) 380, 924, 1325, 1555
MseI TTAA 2 cut(s) 899, 1011
MslI CAYNNNNRTG 2 cut(s) 1058, 1535
MspI CCGG 2 cut(s) 279, 798
MspR9I CCNGG 5 cut(s) 155, 212, 279, 837, 1662
MunI CAATTG 1 cut(s) 779
Mva1269I GAATGC 1 cut(s) 155
MvaI CCWGG 4 cut(s) 155, 212, 837, 1662
MvnI CGCG 1 cut(s) 1144
MwoI GCNNNNNNNGC 5 cut(s) 340, 700, 709, 1238, 1608
NciI CCSGG 1 cut(s) 279
NdeII GATC 9 cut(s) 106, 327, 436, 772, 832, 892, 1045, 1063, 1134
NlaIII CATG 9 cut(s) 57, 352, 757, 794, 976, 1021, 1043, 1057, 1063
NlaIV GGNNCC 3 cut(s) 1136, 1344, 1581
NmuCI GTSAC 1 cut(s) 255
PaeR7I CTCGAG 1 cut(s) 1242
PagI TCATGA 2 cut(s) 1017, 1053
PceI AGGCCT 1 cut(s) 1434
PcsI WCGNNNNNNNCGW 1 cut(s) 1234
PctI GAATGC 1 cut(s) 155
PdmI GAANNNNTTC 4 cut(s) 380, 924, 1325, 1555
PfeI GAWTC 4 cut(s) 323, 674, 748, 979
PflFI GACNNNGTC 1 cut(s) 1662
PflMI CCANNNNNTGG 1 cut(s) 217
PfoI TCCNGGA 1 cut(s) 835
PkrI GCNGC 4 cut(s) 184, 318, 702, 1484
PleI GAGTC 2 cut(s) 477, 563
PpsI GAGTC 2 cut(s) 477, 563
Psp124BI GAGCTC 2 cut(s) 202, 948
Psp6I CCWGG 4 cut(s) 153, 210, 835, 1660
PspGI CCWGG 4 cut(s) 153, 210, 835, 1660
PspN4I GGNNCC 3 cut(s) 1136, 1344, 1581
PspPI GGNCC 3 cut(s) 1564, 1580, 1718
PspXI VCTCGAGB 1 cut(s) 1242
PstNI CAGNNNCTG 2 cut(s) 227, 1576
PsuI RGATCY 3 cut(s) 832, 1045, 1134
PsyI GACNNNGTC 1 cut(s) 1662
RsaI GTAC 3 cut(s) 129, 464, 1344
RsaNI GTAC 3 cut(s) 128, 463, 1343
RseI CAYNNNNRTG 2 cut(s) 1058, 1535
SacI GAGCTC 2 cut(s) 202, 948
SaqAI TTAA 2 cut(s) 899, 1011
SatI GCNGC 4 cut(s) 183, 317, 701, 1483
Sau3AI GATC 9 cut(s) 106, 327, 436, 772, 832, 892, 1045, 1063, 1134
Sau96I GGNCC 3 cut(s) 1564, 1580, 1718
SchI GAGTC 2 cut(s) 477, 563
ScrFI CCNGG 5 cut(s) 155, 212, 279, 837, 1662
SduI GDGCHC 5 cut(s) 19, 202, 948, 1213, 1243
SexAI ACCWGGT 1 cut(s) 1660
SfaNI GCATC 2 cut(s) 169, 253
SfcI CTRYAG 2 cut(s) 77, 163
Sfr274I CTCGAG 1 cut(s) 1242
SinI GGWCC 1 cut(s) 1580
SlaI CTCGAG 1 cut(s) 1242
SmiMI CAYNNNNRTG 2 cut(s) 1058, 1535
SmlI CTYRAG 6 cut(s) 193, 509, 929, 947, 1242, 1605
SmoI CTYRAG 6 cut(s) 193, 509, 929, 947, 1242, 1605
SseBI AGGCCT 1 cut(s) 1434
SsiI CCGC 2 cut(s) 514, 700
SspI AATATT 1 cut(s) 558
SspMI CTAG 8 cut(s) 320, 455, 680, 1139, 1296, 1352, 1491, 1680
SstI GAGCTC 2 cut(s) 202, 948
StuI AGGCCT 1 cut(s) 1434
StyD4I CCNGG 5 cut(s) 153, 210, 277, 835, 1660
StyI CCWWGG 3 cut(s) 588, 1429, 1490
TaaI ACNGT 4 cut(s) 270, 644, 670, 1725
TaiI ACGT 2 cut(s) 1231, 1240
TaqI TCGA 3 cut(s) 13, 326, 1243
TauI GCSGC 1 cut(s) 703
TfiI GAWTC 4 cut(s) 323, 674, 748, 979
Tru1I TTAA 2 cut(s) 899, 1011
Tru9I TTAA 2 cut(s) 899, 1011
TscAI CASTG 6 cut(s) 27, 312, 675, 1537, 1704, 1728
TseFI GTSAC 1 cut(s) 255
TseI GCWGC 3 cut(s) 182, 316, 1482
Tsp45I GTSAC 1 cut(s) 255
TspDTI ATGAA 6 cut(s) 108, 516, 929, 1034, 1104, 1701
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 6 cut(s) 27, 312, 675, 1537, 1704, 1728
Tth111I GACNNNGTC 1 cut(s) 1662
Van91I CCANNNNNTGG 1 cut(s) 217
VneI GTGCAC 1 cut(s) 1209
VpaK11BI GGWCC 1 cut(s) 1580
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 4 cut(s) 236, 1260, 1459, 1497
XhoI CTCGAG 1 cut(s) 1242
XmaJI CCTAGG 1 cut(s) 1490
XmnI GAANNNNTTC 4 cut(s) 380, 924, 1325, 1555
XspI CTAG 8 cut(s) 320, 455, 680, 1139, 1296, 1352, 1491, 1680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.