Rh4CG321900

65-kDa microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0007124
Physical Location & Seq
Reverse (-)
58418768 .. 58425336
6569 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG321900.1

Sequence Viewer

Length: 1233 bp
ATGGGTTCATTTCGAGCACCAGTGGGGAGAAGCTCTGCGCTTCCTGAAACATCATGTGGATATTTGCTTCAAGAACTACAGATGATTTGGAATGAAATTGGACAAGATCAGTTGGAAAGGGAGAAGGTACTGGTGGATTTAGAGCAGGAATGCCTGGAGGTCTATAGGAGGAAGGTTGATGCTGCTAACACCTCAAGAGCTCGCCTGCACCAGGAGTTGGCAGAAGCTGAGGCTGAATTTACGCATCTTCTTTTGTCACTTGGCGAACGGTCTCTCCCCGGACGGCCAGAAAAAATGGTGGGAACACTGAAAGAGCAGCTAGATTCGATCACGCCAGCTCTGCGAGACATGAGATTGAGGAAAGAAGATAGGGTGAACAAGTTCCGAGCTGTACAAGGGCAAATTCAGAAGATCTCTGCAGAAATAGCAGGACAGTCAGAGTATGAGAACTCATCATCAGATGTCAGGGTGAATGAGAATGATCTTTCCTTGAAGAAACTAGAGGAGTACCAGACGGAGCTACAAAGACTCCACAATGACAGGAATGAAAGGCTCAAGCGGGTGGAGAAATATATAAACTCCATCCAAAAGTTGTCTTCAATATTAGGAACAGACTCCTCTATGGTCATCACCAAGGTTCACCCAAGCTTGAATGAGTTATGTGGGATAGCCAAGAACATAAGCAACAGTATTTTGGCTAAACTCAACAGCACAGTGGAATCCCTAGAGGAAGAAAAGCAAAAGCGGCTTGAAAAGCTTCACCATCTTGGTAAAGCATTGACAAACTTATGGAATCTCATGGATACACCATATAAAGATCGTCAATTGTTTTCCCATGTTACCGGCTTATTATCAGTCTCATCAGCAGAAGTATCAGATCCAGGAAGCCTAACCCAAAAGATAATCCAGCAGGTACCTCTCCTAGCAATGTTGGAAGAGTATATTGTGTTGAGGCAGGAAAAGGAGGGGGAGAGGCAAAGACAAAGGGAAGTGAAGTTCCAAGGCCTAATAGGGCAACATCAGCCAGAAAATTTGTTCCTGCCCAGTCCAAGCAGCAGTCCTAGGCGAATTTCAAACAGAAGTGTAAATGGAGGCTTTAGCACTGCTATGCCTCTAAACAGAAGACTTTCTCTGGGCCTTCAGAAACTGGGACCAAACAGCATAGATTTAGGCACTCAGGGCATATCAAAGGAAGCGGCAAGAAAAAGTTGCACCCCAAAGACATTTACCTAG

Protein Analysis

410

Amino Acids

46.34

Weight (kDa)

8.71

Isoelectric Point (pI)

52.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 22 - 302 3.3e-33 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 901
Acc65I GGTACC 1 cut(s) 913
AccB1I GGYRCC 1 cut(s) 913
AccB7I CCANNNNNTGG 1 cut(s) 217
AciI CCGC 3 cut(s) 559, 745, 1196
AclWI GGATC 1 cut(s) 872
AcoI YGGCCR 1 cut(s) 284
AcsI RAATTY 4 cut(s) 236, 402, 1030, 1068
AcuI CTGAAG 1 cut(s) 1124
AfaI GTAC 4 cut(s) 129, 393, 509, 915
AfiI CCNNNNNNNGG 2 cut(s) 211, 217
AgsI TTSAA 6 cut(s) 71, 493, 600, 652, 752, 1074
AjnI CCWGG 3 cut(s) 153, 210, 880
AloI GAACNNNNNNTCC 4 cut(s) 258, 290, 980, 1012
AluBI AGCT 9 cut(s) 33, 200, 227, 319, 338, 389, 520, 648, 757
AluI AGCT 9 cut(s) 33, 200, 227, 319, 338, 389, 520, 648, 757
Alw21I GWGCWC 2 cut(s) 19, 202
Alw26I GTCTC 3 cut(s) 276, 339, 862
AlwI GGATC 1 cut(s) 872
AlwNI CAGNNNCTG 2 cut(s) 227, 1147
AoxI GGCC 3 cut(s) 284, 1003, 1135
ApeKI GCWGC 3 cut(s) 182, 316, 1053
ApoI RAATTY 4 cut(s) 236, 402, 1030, 1068
Asp700I GAANNNNTTC 3 cut(s) 380, 756, 1126
Asp718I GGTACC 1 cut(s) 913
AspA2I CCTAGG 1 cut(s) 1061
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 2 cut(s) 1135, 1151
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 5 cut(s) 385, 481, 622, 632, 752
AvaII GGWCC 1 cut(s) 1151
AvrII CCTAGG 1 cut(s) 1061
BanI GGYRCC 1 cut(s) 913
BanII GRGCYC 1 cut(s) 202
BbsI GAAGAC 2 cut(s) 588, 1129
Bbv12I GWGCWC 2 cut(s) 19, 202
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 3 cut(s) 169, 328, 1065
BccI CCATC 2 cut(s) 590, 771
BceAI ACGGC 1 cut(s) 299
BciT130I CCWGG 3 cut(s) 155, 212, 882
BciVI GTATCC 1 cut(s) 796
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 3 cut(s) 276, 339, 862
BfaI CTAG 6 cut(s) 320, 500, 725, 923, 1062, 1231
BfmI CTRYAG 3 cut(s) 77, 163, 417
BfuAI ACCTGC 1 cut(s) 901
BfuI GTATCC 1 cut(s) 796
BglII AGATCT 1 cut(s) 411
BisI GCNGC 5 cut(s) 183, 317, 746, 1054, 1197
BlnI CCTAGG 1 cut(s) 1061
BlsI GCNGC 5 cut(s) 184, 318, 747, 1055, 1198
Bme1390I CCNGG 4 cut(s) 155, 212, 279, 882
Bme18I GGWCC 1 cut(s) 1151
BmgT120I GGNCC 2 cut(s) 1135, 1151
BmiI GGNNCC 2 cut(s) 915, 1152
BmrFI CCNGG 4 cut(s) 155, 212, 279, 882
BmrI ACTGGG 2 cut(s) 1038, 1157
BmsI GCATC 2 cut(s) 169, 253
BmuI ACTGGG 2 cut(s) 1038, 1157
BpiI GAAGAC 2 cut(s) 588, 1129
BpmI CTGGAG 1 cut(s) 176
Bpu10I CCTNAGC 1 cut(s) 228
BpuEI CTTGAG 2 cut(s) 178, 539
BpuMI CCSGG 1 cut(s) 279
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 4 cut(s) 277, 633, 1000, 1061
BsaXI ACNNNNNCTCC 4 cut(s) 258, 288, 513, 543
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 217
Bse118I RCCGGY 1 cut(s) 842
Bse1I ACTGG 4 cut(s) 20, 135, 1044, 1152
Bse3DI GCAATG 1 cut(s) 933
BseBI CCWGG 3 cut(s) 155, 212, 882
BseDI CCNNGG 4 cut(s) 277, 633, 1000, 1061
BseGI GGATG 1 cut(s) 582
BseLI CCNNNNNNNGG 2 cut(s) 211, 217
BseMI GCAATG 1 cut(s) 933
BseMII CTCAG 2 cut(s) 219, 1190
BseNI ACTGG 4 cut(s) 20, 135, 1044, 1152
BseRI GAGGAG 2 cut(s) 518, 607
BseXI GCAGC 3 cut(s) 169, 328, 1065
BsgI GTGCAG 1 cut(s) 191
BshFI GGCC 3 cut(s) 286, 1005, 1137
BshNI GGYRCC 1 cut(s) 913
BsiHKAI GWGCWC 2 cut(s) 19, 202
BsiSI CCGG 2 cut(s) 279, 843
BslFI GGGAC 1 cut(s) 1164
BslI CCNNNNNNNGG 2 cut(s) 211, 217
BsmAI GTCTC 3 cut(s) 276, 339, 862
BsmFI GGGAC 1 cut(s) 1164
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 3 cut(s) 286, 1005, 1137
Bso31I GGTCTC 1 cut(s) 276
Bsp1286I GDGCHC 2 cut(s) 19, 202
Bsp1407I TGTACA 1 cut(s) 391
Bsp143I GATC 6 cut(s) 106, 327, 411, 481, 817, 877
BspACI CCGC 3 cut(s) 559, 745, 1196
BspANI GGCC 3 cut(s) 286, 1005, 1137
BspCNI CTCAG 2 cut(s) 220, 1189
BspLI GGNNCC 2 cut(s) 915, 1152
BspMAI CTGCAG 1 cut(s) 421
BspMI ACCTGC 1 cut(s) 901
BspPI GGATC 1 cut(s) 872
BspT107I GGYRCC 1 cut(s) 913
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 933
BsrFI RCCGGY 1 cut(s) 842
BsrGI TGTACA 1 cut(s) 391
BsrI ACTGG 4 cut(s) 20, 135, 1044, 1152
BssAI RCCGGY 1 cut(s) 842
BssECI CCNNGG 4 cut(s) 277, 633, 1000, 1061
BssMI GATC 6 cut(s) 106, 327, 411, 481, 817, 877
BssT1I CCWWGG 3 cut(s) 633, 1000, 1061
Bst2UI CCWGG 3 cut(s) 155, 212, 882
Bst4CI ACNGT 4 cut(s) 270, 435, 689, 715
Bst6I CTCTTC 1 cut(s) 930
BstAUI TGTACA 1 cut(s) 391
BstC8I GCNNGC 3 cut(s) 202, 206, 336
BstDEI CTNAG 2 cut(s) 228, 1176
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 1 cut(s) 582
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 6 cut(s) 109, 330, 414, 484, 820, 880
BstMAI GTCTC 3 cut(s) 276, 339, 862
BstMBI GATC 6 cut(s) 106, 327, 411, 481, 817, 877
BstMWI GCNNNNNNNGC 6 cut(s) 340, 425, 745, 754, 1021, 1179
BstNI CCWGG 3 cut(s) 155, 212, 882
BstSCI CCNGG 4 cut(s) 153, 210, 277, 880
BstSFI CTRYAG 3 cut(s) 77, 163, 417
BstV1I GCAGC 3 cut(s) 169, 328, 1065
BstV2I GAAGAC 2 cut(s) 588, 1129
BstX2I RGATCY 2 cut(s) 411, 877
BstYI RGATCY 2 cut(s) 411, 877
BsuI GTATCC 1 cut(s) 796
BsuRI GGCC 3 cut(s) 286, 1005, 1137
BtsCI GGATG 1 cut(s) 582
BtsI GCAGTG 1 cut(s) 1101
BtsIMutI CAGTG 4 cut(s) 27, 305, 720, 1101
BveI ACCTGC 1 cut(s) 901
Cac8I GCNNGC 3 cut(s) 202, 206, 336
CaiI CAGNNNCTG 2 cut(s) 227, 1147
CfoI GCGC 1 cut(s) 40
Cfr10I RCCGGY 1 cut(s) 842
Cfr13I GGNCC 2 cut(s) 1135, 1151
Csp6I GTAC 4 cut(s) 128, 392, 508, 914
CviAII CATG 4 cut(s) 54, 349, 799, 836
CviQI GTAC 4 cut(s) 128, 392, 508, 914
DdeI CTNAG 2 cut(s) 228, 1176
DpnI GATC 6 cut(s) 108, 329, 413, 483, 819, 879
DpnII GATC 6 cut(s) 106, 327, 411, 481, 817, 877
EaeI YGGCCR 1 cut(s) 284
Eam1104I CTCTTC 1 cut(s) 930
EarI CTCTTC 1 cut(s) 930
Ecl136II GAGCTC 1 cut(s) 200
Eco130I CCWWGG 3 cut(s) 633, 1000, 1061
Eco147I AGGCCT 1 cut(s) 1005
Eco24I GRGCYC 1 cut(s) 202
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 1151
Eco53kI GAGCTC 1 cut(s) 200
Eco57I CTGAAG 1 cut(s) 1124
EcoICRI GAGCTC 1 cut(s) 200
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoRII CCWGG 3 cut(s) 153, 210, 880
EcoT14I CCWWGG 3 cut(s) 633, 1000, 1061
EcoT38I GRGCYC 1 cut(s) 202
ErhI CCWWGG 3 cut(s) 633, 1000, 1061
FaeI CATG 4 cut(s) 57, 352, 802, 839
FaqI GGGAC 1 cut(s) 1164
FatI CATG 4 cut(s) 53, 348, 798, 835
FauI CCCGC 1 cut(s) 552
Fnu4HI GCNGC 5 cut(s) 183, 317, 746, 1054, 1197
FokI GGATG 1 cut(s) 569
FriOI GRGCYC 1 cut(s) 202
Fsp4HI GCNGC 5 cut(s) 183, 317, 746, 1054, 1197
FspBI CTAG 6 cut(s) 320, 500, 725, 923, 1062, 1231
GlaI GCGC 1 cut(s) 39
GluI GCNGC 5 cut(s) 183, 317, 746, 1054, 1197
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 3 cut(s) 286, 1005, 1137
HapII CCGG 2 cut(s) 279, 843
HhaI GCGC 1 cut(s) 40
Hin1II CATG 4 cut(s) 57, 352, 802, 839
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HindIII AAGCTT 2 cut(s) 646, 755
HinfI GANTC 5 cut(s) 323, 528, 614, 719, 793
HpaII CCGG 2 cut(s) 279, 843
HphI GGTGA 5 cut(s) 385, 481, 622, 632, 752
Hpy166II GTNNAC 2 cut(s) 376, 640
Hpy188I TCNGA 6 cut(s) 386, 408, 439, 460, 877, 1143
Hpy188III TCNNGA 3 cut(s) 44, 71, 195
Hpy8I GTNNAC 2 cut(s) 376, 640
HpyAV CCTTC 3 cut(s) 118, 166, 1148
HpyCH4III ACNGT 4 cut(s) 270, 435, 689, 715
HpyCH4V TGCA 3 cut(s) 208, 419, 1212
HpyF10VI GCNNNNNNNGC 6 cut(s) 340, 425, 745, 754, 1021, 1179
HpyF3I CTNAG 2 cut(s) 228, 1176
Hsp92II CATG 4 cut(s) 57, 352, 802, 839
HspAI GCGC 1 cut(s) 38
KpnI GGTACC 1 cut(s) 917
Kzo9I GATC 6 cut(s) 106, 327, 411, 481, 817, 877
LmnI GCTCC 1 cut(s) 517
Lsp1109I GCAGC 3 cut(s) 169, 328, 1065
LweI GCATC 2 cut(s) 169, 253
MaeI CTAG 6 cut(s) 320, 500, 725, 923, 1062, 1231
MaeIII GTNAC 2 cut(s) 255, 838
MalI GATC 6 cut(s) 108, 329, 413, 483, 819, 879
MboI GATC 6 cut(s) 106, 327, 411, 481, 817, 877
MboII GAAGA 8 cut(s) 239, 377, 421, 505, 588, 743, 947, 1134
MfeI CAATTG 1 cut(s) 824
MflI RGATCY 2 cut(s) 411, 877
MhlI GDGCHC 2 cut(s) 19, 202
MluCI AATT 6 cut(s) 96, 236, 402, 824, 1030, 1068
MlyI GAGTC 2 cut(s) 522, 608
MmeI TCCRAC 2 cut(s) 93, 912
MroXI GAANNNNTTC 3 cut(s) 380, 756, 1126
MslI CAYNNNNRTG 1 cut(s) 1106
MspI CCGG 2 cut(s) 279, 843
MspR9I CCNGG 4 cut(s) 155, 212, 279, 882
MunI CAATTG 1 cut(s) 824
Mva1269I GAATGC 1 cut(s) 155
MvaI CCWGG 3 cut(s) 155, 212, 882
MwoI GCNNNNNNNGC 6 cut(s) 340, 425, 745, 754, 1021, 1179
NciI CCSGG 1 cut(s) 279
NdeII GATC 6 cut(s) 106, 327, 411, 481, 817, 877
NlaIII CATG 4 cut(s) 57, 352, 802, 839
NlaIV GGNNCC 2 cut(s) 915, 1152
NmuCI GTSAC 1 cut(s) 255
PceI AGGCCT 1 cut(s) 1005
PctI GAATGC 1 cut(s) 155
PdmI GAANNNNTTC 3 cut(s) 380, 756, 1126
PfeI GAWTC 3 cut(s) 323, 719, 793
PflMI CCANNNNNTGG 1 cut(s) 217
PfoI TCCNGGA 1 cut(s) 880
PkrI GCNGC 5 cut(s) 184, 318, 747, 1055, 1198
PleI GAGTC 2 cut(s) 522, 608
PpsI GAGTC 2 cut(s) 522, 608
Psp124BI GAGCTC 1 cut(s) 202
Psp6I CCWGG 3 cut(s) 153, 210, 880
PspGI CCWGG 3 cut(s) 153, 210, 880
PspN4I GGNNCC 2 cut(s) 915, 1152
PspPI GGNCC 2 cut(s) 1135, 1151
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 2 cut(s) 227, 1147
PsuI RGATCY 2 cut(s) 411, 877
RsaI GTAC 4 cut(s) 129, 393, 509, 915
RsaNI GTAC 4 cut(s) 128, 392, 508, 914
RseI CAYNNNNRTG 1 cut(s) 1106
SacI GAGCTC 1 cut(s) 202
SatI GCNGC 5 cut(s) 183, 317, 746, 1054, 1197
Sau3AI GATC 6 cut(s) 106, 327, 411, 481, 817, 877
Sau96I GGNCC 2 cut(s) 1135, 1151
SchI GAGTC 2 cut(s) 522, 608
ScrFI CCNGG 4 cut(s) 155, 212, 279, 882
SduI GDGCHC 2 cut(s) 19, 202
SfaNI GCATC 2 cut(s) 169, 253
SfcI CTRYAG 3 cut(s) 77, 163, 417
SinI GGWCC 1 cut(s) 1151
SmiMI CAYNNNNRTG 1 cut(s) 1106
SmlI CTYRAG 2 cut(s) 193, 554
SmoI CTYRAG 2 cut(s) 193, 554
Sse9I AATT 6 cut(s) 96, 236, 402, 824, 1030, 1068
SseBI AGGCCT 1 cut(s) 1005
SsiI CCGC 3 cut(s) 559, 745, 1196
SspI AATATT 1 cut(s) 603
SspMI CTAG 6 cut(s) 320, 500, 725, 923, 1062, 1231
SstI GAGCTC 1 cut(s) 202
StuI AGGCCT 1 cut(s) 1005
StyD4I CCNGG 4 cut(s) 153, 210, 277, 880
StyI CCWWGG 3 cut(s) 633, 1000, 1061
TaaI ACNGT 4 cut(s) 270, 435, 689, 715
TaqI TCGA 2 cut(s) 13, 326
TasI AATT 6 cut(s) 96, 236, 402, 824, 1030, 1068
TatI WGTACW 1 cut(s) 391
TauI GCSGC 2 cut(s) 748, 1199
TfiI GAWTC 3 cut(s) 323, 719, 793
TscAI CASTG 4 cut(s) 27, 312, 720, 1108
TseFI GTSAC 1 cut(s) 255
TseI GCWGC 3 cut(s) 182, 316, 1053
Tsp45I GTSAC 1 cut(s) 255
TspDTI ATGAA 2 cut(s) 108, 561
TspGWI ACGGA 1 cut(s) 530
TspRI CASTG 4 cut(s) 27, 312, 720, 1108
Van91I CCANNNNNTGG 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 1151
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 4 cut(s) 236, 402, 1030, 1068
XmaJI CCTAGG 1 cut(s) 1061
XmnI GAANNNNTTC 3 cut(s) 380, 756, 1126
XspI CTAG 6 cut(s) 320, 500, 725, 923, 1062, 1231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.