Rorug04G0242800

65-kDa microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007124
Physical Location & Seq
Forward (+)
41171529 .. 41174846
3318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0242800.1

Sequence Viewer

Length: 3000 bp
ATGGTTGAAGCAGTTCCATTTGGCACTGCAGCTGACATACTCATCAAGCTCGGGTCTTCCACTTTCCGGGAGCTCGGGCTCGTCTACGGCGTCAACAAGGAAATGAAGAAGCTTGAGAACACTCTCTCCACCATCAAAGCTGTTCTCTTAGACGCAGAGGAGAAGCAAGAGAAGAGCCATCTGGTGCAGGACTGGATCAGGAAGCTCAGCGAGGTTGTGTACGAGGCCGATGATTTGTTGGATGCATTCGAAACCAAAGCTAGGCAGCATGAGAATCATCAGGTGCGTGAGTTCTTTTCAAAGACACTTGCTTTTAGATACAAGATGGGTCGAAAACTGAGAGAAACTAGAGAGAGATTGGATGAGATTGCAGGGGATATTTCAAAGTTCAACTTCAGGGATAGGTCGTTGACAATCTCAGATATTGGGATTGAGATGAATAATATGAGAGAAGAGACGCATTCTTTTATGGTGGAGTCAGCTGTAATTGGAAGAAAGAGAGAGAAGGAGGAGATGGTGGAGTTTTTGATATTCCCACCAAGTGAAGGAGAAAATGTCTCTATTACTGCCGTTGTTGGTATTGGGGGGCTTGGGAAGACCACCCTTGCTAAAATGTTGTATAATGATGATAGGGTAGTGAAGTATTTTGAGATTAAGATGTGGGTGTGGGCGTATGGAAGTTTTGATTTATATTCAGTTACGGAGAAAATCTTGAGATCTAGTGCTACGAATGAACTAGAAGATTCGGAGTTTTATTACCAGCATATTTTGCTGCAGAAGAAAATGTTGATGGAGGAGTTGCAGATTCTGCTTCGGAAGAATTTGGATGGGAAGCGCTACCTCCTTGTTCTGGATGATGTGTGGAATGTGAAGCGCGAAAGTTGGCTTCAGCTGAATGATTTGTTGAAAGGTGGTGCCAAGGGGAGTAAGATTGTTGTGACAACTCGTAGCACAAAAGTTACCGCGCTTATGGATGTTGATTTCCCTTTTGTGTTGCAAGATCTATCGGAGGAAGAGAGTTGGACATTGTTTAAGCAACTGGCTTTTAAAGATGGAAGGGAGGAGGAGCATCCAGAGTTGGTGCCAATAGGAAGAAGAATTGTGAAGAAATGCAGAGGTGTTCCTCTTGCAATAAGGACTCTAGGAAGCATGATGAGATACAAAGCTAATGAAAGAGAGTGGATTTCGATTCAGAATACAGAGATCTGGGAGTTACCAAAAGAGGAGAATGATGTTTTGAAGATACTGAAGTTAAGCTATGATCACATGCCAATTTATTTGAAACAATGCTTCTCCTACTGCGCATTGTTCCCCAAGGGATATGAAATTGAGAAAAAAATGTTGGTCGAACTTTGGATAGCTCAGGGTTACATTCACCTTCCTGGTAAAGAGAGGAACTTGGAAGAAATTGGTGACCTGTATTTCAAGGAATTGATAGAGAGATCATTATTTCAAGAGGTCAAGGAAGATGCCAATGGAAATTTAATTAGTTGTCATATGCATGATCTTATACACGATCTTTCACGGGCAGTTGCAGGATCTGAATGCTCAATTTGCAGTGTTGATGTTGGAGATATATCTGAAAGAGTTCATCATGTTTCATTTAGTTGTTATCTGCCTTCTTCATGGGAAGTTCCGATAGCGTTGCTCAAAGCTAAGAAGACTAGGACATTTATACTACCACTTCAGGATCAAGCATATAGAATTTCCAGTCACAATACAATCATTTCAAACTTGAGGTGCTTACGTGTGTTAGATCTACATAACCTAAGAATCGATGAGCTTTCAAATTCCGTAGGCAAGTTGATCCATCTTAGGTACCTTGACCTCTCTGGTAATGTTAGTATGACAGCACTCCCTGCTTCTATCAGCAATCTGCAGAACCTACAGACCCTTAAACTCAACAGGTGCTACAGACTTGAACACCTACCTAGAGATATCGGAAAGATGATCAGCCTTAGACATCTTGAGATCACTGGGTGCCGCAAATTGAATCACATGCCACCTGGGCTGGGAAAGTTGGTATCTCTTCAGACATTACCGTTATTCATCTTGGGCAGGAAAGCTCTCTTTTCCGGTGTGAGTGGTGGAATAGATGAGTTGAGTGGCTTGAACTGTCTTAAAGGGGTGCTTCATGTTGCCCATCTTGAACATGTTGACAATGTCACAATAGATTCAAGGAGTTCAAATTTGAGCAGCAAGCAATATCTTGAATCCTTAAAACTAATTTGGTCCAGAGATGACGACAGTGAAAGTAATGAAGATCACATGGTGTTGGAAGGCCTTCAGCCCCATCAAAATTTGAAAGTACTGCATATAGCTGGGTATTGCGACGTTCAATTCCCCAACTGGCTGATGAACAACGCTGCATCGTCTCTTCCAAGTCTAGTTGAGCTCACAATTGAGGGCTGTCTCAGATGCCAGCATCTTCCACCACTGGATCAACTGCCTTCTCTTAAGTTTCTCAAACTATATTCTCTAGGTTGCCTCGAGCACATTAACAGCAGTTACTCGTCTCCCTCTTGTGGTTTGGTTGGGGGAGGAAGAGAGCCTTTGTTTTTTCCATCATTGAAAGAGCTCACACTTTATGACTTGCCACTCTTGAAGGAGTGGCAAACCTGCAGTGATCAAGATGGGACAACAACAATGCCAAAGCAGCAGCAGCTTTCATTTCCTTCCCTCACCAAATTGACTATCGTGGACGCCCCTAACTTAATGTCCATGCCGATGCTTCCATTAGTTGAAGAATTGCTTATGGACACCGTCTCTGAGAAGTTGTTGCAGTCCATGTTGATGACAGCAGCCTCAGAAAGCCAAACAAGCTCTTCCTCCCTTGCTAGATTGAAATTTCTGATCATCAAATCATGCAATGATCTGGTCTCTTTCCCTGAAGATAGGATAAATAACCTCACTTCTCTTAAGCTTCTAAAGATTATAGATTGTGGAAACTTGACATCTCTACCACATAATCTGCACTGTGAATACGTACTTAGTAATTAG

Protein Analysis

999

Amino Acids

113.81

Weight (kDa)

6.08

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 15 - 97 4.2e-22 Rx N-terminal domain
NB-ARC PF00931 173 - 350 4.8e-30 NB-ARC domain
WHD_DRP PF23559 436 - 507 7.9e-26 Disease resistance protein Winged helix domain
LRR_14 PF23598 575 - 893 1e-33 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 698 - 826 2.5e-33 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1303
Acc36I ACCTGC 1 cut(s) 2627
Acc65I GGTACC 1 cut(s) 1818
AccB1I GGYRCC 4 cut(s) 914, 1081, 1818, 1980
AccI GTMKAC 1 cut(s) 84
AccII CGCG 2 cut(s) 876, 965
AciI CCGC 2 cut(s) 963, 1984
AclWI GGATC 5 cut(s) 203, 1546, 1698, 1801, 2448
AcsI RAATTY 7 cut(s) 820, 1480, 1704, 1789, 2188, 2299, 2846
AcuI CTGAAG 7 cut(s) 379, 872, 1268, 1670, 2015, 2270, 2910
AcyI GRCGYC 2 cut(s) 90, 2703
AdeI CACNNNGTG 3 cut(s) 542, 1980, 2272
AfaI GTAC 4 cut(s) 221, 1820, 2310, 2988
AfeI AGCGCT 1 cut(s) 836
AfiI CCNNNNNNNGG 6 cut(s) 66, 261, 545, 850, 2012, 2525
AflII CTTAAG 2 cut(s) 2456, 2918
AflIII ACRYGT 2 cut(s) 1747, 2152
AjnI CCWGG 2 cut(s) 1381, 2005
AjuI GAANNNNNNNTTGG 2 cut(s) 1325, 1357
AloI GAACNNNNNNTCC 2 cut(s) 110, 142
Alw21I GWGCWC 4 cut(s) 75, 2397, 2496, 2580
Alw26I GTCTC 7 cut(s) 449, 562, 2379, 2417, 2520, 2770, 2884
AlwI GGATC 5 cut(s) 203, 1546, 1698, 1801, 2448
AlwNI CAGNNNCTG 2 cut(s) 808, 1541
Ama87I CYCGRG 3 cut(s) 50, 74, 2489
Aor51HI AGCGCT 1 cut(s) 836
AoxI GGCC 2 cut(s) 225, 2281
ApeKI GCWGC 9 cut(s) 29, 265, 772, 2196, 2366, 2656, 2659, 2662, 2801
ApoI RAATTY 7 cut(s) 820, 1480, 1704, 1789, 2188, 2299, 2846
Asp700I GAANNNNTTC 3 cut(s) 12, 1587, 2283
Asp718I GGTACC 1 cut(s) 1818
AspLEI GCGC 4 cut(s) 837, 876, 967, 1304
AspS9I GGNCC 1 cut(s) 2232
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 3 cut(s) 1367, 1424, 2674
AsuII TTCGAA 1 cut(s) 249
AvaI CYCGRG 3 cut(s) 50, 74, 2489
AvaII GGWCC 1 cut(s) 2232
BaeI ACNNNNGTAYC 2 cut(s) 1235, 1268
BanI GGYRCC 4 cut(s) 914, 1081, 1818, 1980
BanII GRGCYC 4 cut(s) 75, 81, 2397, 2580
BbsI GAAGAC 3 cut(s) 48, 602, 1667
Bbv12I GWGCWC 4 cut(s) 75, 2397, 2496, 2580
BbvI GCAGC 9 cut(s) 41, 277, 759, 2208, 2353, 2668, 2671, 2674, 2813
BceAI ACGGC 2 cut(s) 103, 554
BcgI CGANNNNNNTGC 2 cut(s) 1627, 1661
BciT130I CCWGG 2 cut(s) 1383, 2007
BclI TGATCA 4 cut(s) 1261, 1950, 2626, 2853
BcnI CCSGG 1 cut(s) 68
BcoDI GTCTC 7 cut(s) 449, 562, 2379, 2417, 2520, 2770, 2884
BfmI CTRYAG 6 cut(s) 27, 773, 1877, 1886, 1912, 2620
BfoI RGCGCY 1 cut(s) 838
BfrI CTTAAG 2 cut(s) 2456, 2918
BfuAI ACCTGC 1 cut(s) 2627
BglI GCCNNNNNGGC 1 cut(s) 2008
BglII AGATCT 4 cut(s) 716, 1000, 1203, 1756
BlpI GCTNAGC 1 cut(s) 206
BmcAI AGTACT 1 cut(s) 2310
Bme1390I CCNGG 3 cut(s) 68, 1383, 2007
Bme18I GGWCC 1 cut(s) 2232
BmeT110I CYCGRG 3 cut(s) 50, 74, 2489
BmgT120I GGNCC 1 cut(s) 2232
BmiI GGNNCC 4 cut(s) 916, 1083, 1820, 1982
BmrFI CCNGG 3 cut(s) 68, 1383, 2007
BmrI ACTGGG 1 cut(s) 1986
BmsI GCATC 7 cut(s) 232, 1078, 1459, 2378, 2408, 2434, 2718
BmuI ACTGGG 1 cut(s) 1986
BpiI GAAGAC 3 cut(s) 48, 602, 1667
Bpu10I CCTNAGC 1 cut(s) 1362
Bpu1102I GCTNAGC 1 cut(s) 206
Bpu14I TTCGAA 1 cut(s) 249
BpuEI CTTGAG 4 cut(s) 134, 733, 1756, 1988
BpuMI CCSGG 1 cut(s) 68
Bsa29I ATCGAT 1 cut(s) 1776
BsaAI YACGTR 2 cut(s) 1748, 2986
BsaHI GRCGYC 2 cut(s) 90, 2703
BsaI GGTCTC 1 cut(s) 2884
BsaJI CCNNGG 3 cut(s) 918, 1314, 2006
BsaWI WCCGGW 1 cut(s) 2075
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 540, 570
Bsc4I CCNNNNNNNGG 6 cut(s) 66, 261, 545, 850, 2012, 2525
Bse1I ACTGG 6 cut(s) 197, 1044, 1710, 1981, 2354, 2442
Bse3DI GCAATG 1 cut(s) 2875
BseBI CCWGG 2 cut(s) 1383, 2007
BseCI ATCGAT 1 cut(s) 1776
BseDI CCNNGG 3 cut(s) 918, 1314, 2006
BseGI GGATG 6 cut(s) 247, 367, 832, 859, 979, 1069
BseLI CCNNNNNNNGG 6 cut(s) 66, 261, 545, 850, 2012, 2525
BseMI GCAATG 1 cut(s) 2875
BseMII CTCAG 7 cut(s) 220, 329, 432, 1376, 2428, 2760, 2820
BseNI ACTGG 6 cut(s) 197, 1044, 1710, 1981, 2354, 2442
BseRI GAGGAG 6 cut(s) 173, 524, 809, 1076, 1079, 1238
BseXI GCAGC 9 cut(s) 41, 277, 759, 2208, 2353, 2668, 2671, 2674, 2813
BseYI CCCAGC 2 cut(s) 2011, 2321
BsgI GTGCAG 2 cut(s) 206, 2957
Bsh1236I CGCG 2 cut(s) 876, 965
BshFI GGCC 2 cut(s) 227, 2283
BshNI GGYRCC 4 cut(s) 914, 1081, 1818, 1980
BshVI ATCGAT 1 cut(s) 1776
BsiHKAI GWGCWC 4 cut(s) 75, 2397, 2496, 2580
BsiHKCI CYCGRG 3 cut(s) 50, 74, 2489
BsiSI CCGG 2 cut(s) 67, 2076
BslFI GGGAC 1 cut(s) 2650
BslI CCNNNNNNNGG 6 cut(s) 66, 261, 545, 850, 2012, 2525
BsmAI GTCTC 7 cut(s) 449, 562, 2379, 2417, 2520, 2770, 2884
BsmBI CGTCTC 4 cut(s) 449, 2379, 2520, 2770
BsmFI GGGAC 1 cut(s) 2650
BsmI GAATGC 3 cut(s) 245, 460, 1550
BsnI GGCC 2 cut(s) 227, 2283
Bso31I GGTCTC 1 cut(s) 2884
BsoBI CYCGRG 3 cut(s) 50, 74, 2489
Bsp119I TTCGAA 1 cut(s) 249
Bsp1286I GDGCHC 5 cut(s) 75, 81, 2397, 2496, 2580
Bsp1720I GCTNAGC 1 cut(s) 206
BspACI CCGC 2 cut(s) 963, 1984
BspANI GGCC 2 cut(s) 227, 2283
BspCNI CTCAG 7 cut(s) 219, 330, 431, 1375, 2427, 2761, 2819
BspDI ATCGAT 1 cut(s) 1776
BspFNI CGCG 2 cut(s) 876, 965
BspLI GGNNCC 4 cut(s) 916, 1083, 1820, 1982
BspMAI CTGCAG 4 cut(s) 31, 777, 1881, 2624
BspMI ACCTGC 1 cut(s) 2627
BspPI GGATC 5 cut(s) 203, 1546, 1698, 1801, 2448
BspQI GCTCTTC 2 cut(s) 167, 2830
BspT104I TTCGAA 1 cut(s) 249
BspT107I GGYRCC 4 cut(s) 914, 1081, 1818, 1980
BspTI CTTAAG 2 cut(s) 2456, 2918
BspTNI GGTCTC 1 cut(s) 2884
BsrDI GCAATG 1 cut(s) 2875
BsrI ACTGG 6 cut(s) 197, 1044, 1710, 1981, 2354, 2442
BssECI CCNNGG 3 cut(s) 918, 1314, 2006
BssNI GRCGYC 2 cut(s) 90, 2703
BssT1I CCWWGG 2 cut(s) 918, 1314
Bst2UI CCWGG 2 cut(s) 1383, 2007
Bst4CI ACNGT 5 cut(s) 2043, 2117, 2249, 2764, 2978
Bst6I CTCTTC 7 cut(s) 167, 447, 1008, 2034, 2382, 2539, 2830
BstACI GRCGYC 2 cut(s) 90, 2703
BstAFI CTTAAG 2 cut(s) 2456, 2918
BstAPI GCANNNNNTGC 3 cut(s) 769, 808, 1859
BstBAI YACGTR 2 cut(s) 1748, 2986
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 2 cut(s) 2201, 2423
BstEII GGTNACC 1 cut(s) 1412
BstF5I GGATG 6 cut(s) 247, 367, 832, 859, 979, 1069
BstFNI CGCG 2 cut(s) 876, 965
BstH2I RGCGCY 1 cut(s) 838
BstHHI GCGC 4 cut(s) 837, 876, 967, 1304
BstMAI GTCTC 7 cut(s) 449, 562, 2379, 2417, 2520, 2770, 2884
BstMWI GCNNNNNNNGC 8 cut(s) 769, 808, 1554, 1859, 2008, 2656, 2662, 2820
BstNI CCWGG 2 cut(s) 1383, 2007
BstNSI RCATGY 3 cut(s) 1270, 2002, 2156
BstPI GGTNACC 1 cut(s) 1412
BstSCI CCNGG 3 cut(s) 66, 1381, 2005
BstSFI CTRYAG 6 cut(s) 27, 773, 1877, 1886, 1912, 2620
BstSNI TACGTA 1 cut(s) 2986
BstUI CGCG 2 cut(s) 876, 965
BstV1I GCAGC 9 cut(s) 41, 277, 759, 2208, 2353, 2668, 2671, 2674, 2813
BstV2I GAAGAC 3 cut(s) 48, 602, 1667
BstX2I RGATCY 5 cut(s) 716, 1000, 1203, 1538, 1756
BstYI RGATCY 5 cut(s) 716, 1000, 1203, 1538, 1756
Bsu15I ATCGAT 1 cut(s) 1776
BsuRI GGCC 2 cut(s) 227, 2283
BsuTUI ATCGAT 1 cut(s) 1776
BtsCI GGATG 6 cut(s) 247, 367, 832, 859, 979, 1069
BtsI GCAGTG 3 cut(s) 24, 1564, 2629
BtsIMutI CAGTG 7 cut(s) 24, 1564, 1974, 2254, 2435, 2629, 2974
BveI ACCTGC 1 cut(s) 2627
Cac8I GCNNGC 2 cut(s) 2201, 2423
CaiI CAGNNNCTG 2 cut(s) 808, 1541
CfoI GCGC 4 cut(s) 837, 876, 967, 1304
Cfr13I GGNCC 1 cut(s) 2232
ClaI ATCGAT 1 cut(s) 1776
CseI GACGC 4 cut(s) 79, 161, 466, 2711
Csp6I GTAC 4 cut(s) 220, 1819, 2309, 2987
CviQI GTAC 4 cut(s) 220, 1819, 2309, 2987
DraI TTTAAA 1 cut(s) 1048
DraIII CACNNNGTG 3 cut(s) 542, 1980, 2272
Eam1104I CTCTTC 7 cut(s) 167, 447, 1008, 2034, 2382, 2539, 2830
EarI CTCTTC 7 cut(s) 167, 447, 1008, 2034, 2382, 2539, 2830
Ecl136II GAGCTC 3 cut(s) 73, 2395, 2578
Eco105I TACGTA 1 cut(s) 2986
Eco130I CCWWGG 2 cut(s) 918, 1314
Eco147I AGGCCT 1 cut(s) 2283
Eco24I GRGCYC 4 cut(s) 75, 81, 2397, 2580
Eco31I GGTCTC 1 cut(s) 2884
Eco32I GATATC 1 cut(s) 1939
Eco47I GGWCC 1 cut(s) 2232
Eco47III AGCGCT 1 cut(s) 836
Eco53kI GAGCTC 3 cut(s) 73, 2395, 2578
Eco57I CTGAAG 7 cut(s) 379, 872, 1268, 1670, 2015, 2270, 2910
Eco88I CYCGRG 3 cut(s) 50, 74, 2489
Eco91I GGTNACC 1 cut(s) 1412
EcoICRI GAGCTC 3 cut(s) 73, 2395, 2578
EcoO65I GGTNACC 1 cut(s) 1412
EcoRII CCWGG 2 cut(s) 1381, 2005
EcoRV GATATC 1 cut(s) 1939
EcoT14I CCWWGG 2 cut(s) 918, 1314
EcoT22I ATGCAT 2 cut(s) 247, 1503
EcoT38I GRGCYC 4 cut(s) 75, 81, 2397, 2580
ErhI CCWWGG 2 cut(s) 918, 1314
Esp3I CGTCTC 4 cut(s) 449, 2379, 2520, 2770
FalI AAGNNNNNCTT 8 cut(s) 377, 409, 2115, 2147, 2536, 2568, 2736, 2768
FaqI GGGAC 1 cut(s) 2650
FauNDI CATATG 1 cut(s) 1497
FbaI TGATCA 4 cut(s) 1261, 1950, 2626, 2853
FblI GTMKAC 1 cut(s) 84
FokI GGATG 6 cut(s) 254, 374, 839, 866, 986, 1056
FriOI GRGCYC 4 cut(s) 75, 81, 2397, 2580
FspI TGCGCA 1 cut(s) 1303
GlaI GCGC 4 cut(s) 836, 875, 966, 1303
GsaI CCCAGC 2 cut(s) 2015, 2325
HaeII RGCGCY 1 cut(s) 838
HaeIII GGCC 2 cut(s) 227, 2283
HapII CCGG 2 cut(s) 67, 2076
HgaI GACGC 4 cut(s) 79, 161, 466, 2711
HhaI GCGC 4 cut(s) 837, 876, 967, 1304
Hin1I GRCGYC 2 cut(s) 90, 2703
Hin6I GCGC 4 cut(s) 835, 874, 965, 1302
HinP1I GCGC 4 cut(s) 835, 874, 965, 1302
HincII GTYRAC 3 cut(s) 94, 411, 2158
HindII GTYRAC 3 cut(s) 94, 411, 2158
HindIII AAGCTT 2 cut(s) 110, 2921
HpaII CCGG 2 cut(s) 67, 2076
HphI GGTGA 3 cut(s) 1367, 1424, 2674
Hpy166II GTNNAC 6 cut(s) 85, 94, 220, 411, 2158, 2701
Hpy8I GTNNAC 6 cut(s) 85, 94, 220, 411, 2158, 2701
Hpy99I CGWCG 1 cut(s) 2336
HpyCH4III ACNGT 5 cut(s) 2043, 2117, 2249, 2764, 2978
HpyCH4IV ACGT 3 cut(s) 1747, 2334, 2985
HpyF10VI GCNNNNNNNGC 8 cut(s) 769, 808, 1554, 1859, 2008, 2656, 2662, 2820
HpySE526I ACGT 3 cut(s) 1747, 2334, 2985
Hsp92I GRCGYC 2 cut(s) 90, 2703
HspAI GCGC 4 cut(s) 835, 874, 965, 1302
KpnI GGTACC 1 cut(s) 1822
Ksp22I TGATCA 4 cut(s) 1261, 1950, 2626, 2853
LguI GCTCTTC 2 cut(s) 167, 2830
LmnI GCTCC 2 cut(s) 70, 1066
Lsp1109I GCAGC 9 cut(s) 41, 277, 759, 2208, 2353, 2668, 2671, 2674, 2813
LweI GCATC 7 cut(s) 232, 1078, 1459, 2378, 2408, 2434, 2718
MaeII ACGT 3 cut(s) 1747, 2334, 2985
MaeIII GTNAC 9 cut(s) 697, 937, 958, 1212, 1367, 1412, 1712, 2164, 2507
MfeI CAATTG 1 cut(s) 2400
MflI RGATCY 5 cut(s) 716, 1000, 1203, 1538, 1756
MhlI GDGCHC 5 cut(s) 75, 81, 2397, 2496, 2580
MlyI GAGTC 2 cut(s) 485, 1132
MmeI TCCRAC 4 cut(s) 219, 1001, 1549, 2256
Mph1103I ATGCAT 2 cut(s) 247, 1503
MroXI GAANNNNTTC 3 cut(s) 12, 1587, 2283
MslI CAYNNNNRTG 3 cut(s) 1500, 2726, 2792
MspA1I CMGCKG 3 cut(s) 32, 482, 892
MspCI CTTAAG 2 cut(s) 2456, 2918
MspI CCGG 2 cut(s) 67, 2076
MspR9I CCNGG 3 cut(s) 68, 1383, 2007
MunI CAATTG 1 cut(s) 2400
Mva1269I GAATGC 3 cut(s) 245, 460, 1550
MvaI CCWGG 2 cut(s) 1383, 2007
MvnI CGCG 2 cut(s) 876, 965
MwoI GCNNNNNNNGC 8 cut(s) 769, 808, 1554, 1859, 2008, 2656, 2662, 2820
NciI CCSGG 1 cut(s) 68
NdeI CATATG 1 cut(s) 1497
NlaIV GGNNCC 4 cut(s) 916, 1083, 1820, 1982
NmuCI GTSAC 4 cut(s) 937, 1412, 1712, 2164
NsbI TGCGCA 1 cut(s) 1303
NsiI ATGCAT 2 cut(s) 247, 1503
NspI RCATGY 3 cut(s) 1270, 2002, 2156
NspV TTCGAA 1 cut(s) 249
PaeR7I CTCGAG 1 cut(s) 2489
PceI AGGCCT 1 cut(s) 2283
PciI ACATGT 1 cut(s) 2152
PciSI GCTCTTC 2 cut(s) 167, 2830
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PctI GAATGC 3 cut(s) 245, 460, 1550
PdmI GAANNNNTTC 3 cut(s) 12, 1587, 2283
PfeI GAWTC 8 cut(s) 274, 743, 805, 1189, 1773, 1993, 2174, 2213
PflFI GACNNNGTC 2 cut(s) 2162, 2762
PfoI TCCNGGA 1 cut(s) 66
PleI GAGTC 2 cut(s) 484, 1132
PpsI GAGTC 2 cut(s) 484, 1132
Ppu21I YACGTR 2 cut(s) 1748, 2986
PscI ACATGT 1 cut(s) 2152
Psp124BI GAGCTC 3 cut(s) 75, 2397, 2580
Psp6I CCWGG 2 cut(s) 1381, 2005
PspEI GGTNACC 1 cut(s) 1412
PspFI CCCAGC 2 cut(s) 2011, 2321
PspGI CCWGG 2 cut(s) 1381, 2005
PspN4I GGNNCC 4 cut(s) 916, 1083, 1820, 1982
PspPI GGNCC 1 cut(s) 2232
PspXI VCTCGAGB 1 cut(s) 2489
PstI CTGCAG 4 cut(s) 31, 777, 1881, 2624
PstNI CAGNNNCTG 2 cut(s) 808, 1541
PsuI RGATCY 5 cut(s) 716, 1000, 1203, 1538, 1756
PsyI GACNNNGTC 2 cut(s) 2162, 2762
PvuII CAGCTG 3 cut(s) 32, 482, 892
RsaI GTAC 4 cut(s) 221, 1820, 2310, 2988
RsaNI GTAC 4 cut(s) 220, 1819, 2309, 2987
RseI CAYNNNNRTG 3 cut(s) 1500, 2726, 2792
SacI GAGCTC 3 cut(s) 75, 2397, 2580
SapI GCTCTTC 2 cut(s) 167, 2830
Sau96I GGNCC 1 cut(s) 2232
ScaI AGTACT 1 cut(s) 2310
SchI GAGTC 2 cut(s) 485, 1132
ScrFI CCNGG 3 cut(s) 68, 1383, 2007
SduI GDGCHC 5 cut(s) 75, 81, 2397, 2496, 2580
SfaNI GCATC 7 cut(s) 232, 1078, 1459, 2378, 2408, 2434, 2718
SfcI CTRYAG 6 cut(s) 27, 773, 1877, 1886, 1912, 2620
Sfr274I CTCGAG 1 cut(s) 2489
SfuI TTCGAA 1 cut(s) 249
SinI GGWCC 1 cut(s) 2232
SlaI CTCGAG 1 cut(s) 2489
SmiMI CAYNNNNRTG 3 cut(s) 1500, 2726, 2792
SmlI CTYRAG 7 cut(s) 113, 712, 1735, 1967, 2456, 2489, 2918
SmoI CTYRAG 7 cut(s) 113, 712, 1735, 1967, 2456, 2489, 2918
SnaBI TACGTA 1 cut(s) 2986
SseBI AGGCCT 1 cut(s) 2283
SsiI CCGC 2 cut(s) 963, 1984
SstI GAGCTC 3 cut(s) 75, 2397, 2580
StuI AGGCCT 1 cut(s) 2283
StyD4I CCNGG 3 cut(s) 66, 1381, 2005
StyI CCWWGG 2 cut(s) 918, 1314
TaaI ACNGT 5 cut(s) 2043, 2117, 2249, 2764, 2978
TaiI ACGT 3 cut(s) 1750, 2337, 2988
TaqI TCGA 6 cut(s) 249, 331, 1187, 1347, 1776, 2490
TatI WGTACW 1 cut(s) 2308
TauI GCSGC 1 cut(s) 1986
TfiI GAWTC 8 cut(s) 274, 743, 805, 1189, 1773, 1993, 2174, 2213
TscAI CASTG 7 cut(s) 31, 1564, 1981, 2254, 2442, 2629, 2981
TseFI GTSAC 4 cut(s) 937, 1412, 1712, 2164
TseI GCWGC 9 cut(s) 29, 265, 772, 2196, 2366, 2656, 2659, 2662, 2801
Tsp45I GTSAC 4 cut(s) 937, 1412, 1712, 2164
TspGWI ACGGA 2 cut(s) 716, 1783
TspRI CASTG 7 cut(s) 31, 1564, 1981, 2254, 2442, 2629, 2981
Tth111I GACNNNGTC 2 cut(s) 2162, 2762
Vha464I CTTAAG 2 cut(s) 2456, 2918
VpaK11BI GGWCC 1 cut(s) 2232
XapI RAATTY 7 cut(s) 820, 1480, 1704, 1789, 2188, 2299, 2846
XceI RCATGY 3 cut(s) 1270, 2002, 2156
XhoI CTCGAG 1 cut(s) 2489
XmiI GTMKAC 1 cut(s) 84
XmnI GAANNNNTTC 3 cut(s) 12, 1587, 2283
ZrmI AGTACT 1 cut(s) 2310
Zsp2I ATGCAT 2 cut(s) 247, 1503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.