MD13G1118200.v1.1

65-kDa microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007124
Physical Location & Seq
Forward (+)
8640086 .. 8644341
4256 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1118200.v1.1.491

Sequence Viewer

Length: 1815 bp
ATGGAGAACACAGAGAAGAAAAACATTCAGATGGGTTCGTTTCAGACGCCGATTGGAATGCGAAGCTCCACGCTTTTGGAAACTTCGTGCGGATACTTGCTTCAGGAATTGCAGATTTGGAATGAAGTTGGACAAGATCAGTTTGAAAGGGAGAAGGTGCTGCTGGATTTGGAGCAGGAGTGTCTGGAGGTTTATAGGAAGAAGGTTGATACTGCGAATACCTCGAGAGCTCGACTGCACCAGGAGTTGGCTGAAGCGGAGGCTGAATTTACCCATCTTCTTCTGTCCCTTGGCGAACGGTCTCTCCCTGGACGGCCAGAAAAAATGGTGGGAACACTGAAAGAACAGTTGGATTCACTCACGCCGGCATTGAGGGAGATGAGATTGAGGAAAGAAGATAGGGTGAATAAGTTCCGAGCAGTACAAGGGCAAATTCAGAAAATTTCTGCAGAAATAGCAGGTCAGTCAGAGTACGAAGACTTATCATCGAATGTCATGGTGAATGAGAATGATCTTTCACCAAAGAAACTAGAGGAGTACCAAACAGAGCTACAAAGACTCCACAACGAGAAGAATGAAAGGCTCATGAGGGTCGAAAAATATATAGATGCAGTCCACAATTTGTCTTTGATATTAGGAACAGACGCCTCAATGGTTATCACCAAGGTTCATCCAAGCTTGAATGAATTATGTGGGATAGCCAAAAATATAAGCAACAGTATTTTGGCTAAACTTAACAGCACAGTGGAATCCCTTGGGGAAGAAAAGCAAAGGCGACTCGAAAAGCTTCACCATCTTGGCAAAGCATTGACAAACTTGTGGGATCTCATGGACACACCCTACAAAGATCGTCAATCGTTTTCCCATGTTACTGGCTTATTATCACTTTCATCAGCAGAAGTATCAGATCCAGGAAGCCTAACCCTACATATAATACAACAGGCTGAGGCTGAAGTCAAGAGACTGGATCAATTAAAAGCAAGCAAGATGAAAGAACTATTTTTCAAGAAACAGAATGAGCTCAAAGAGATACGCAATAAATCACACATGGAGATTCCTTCGCAATCAGAGATTGATAATCTAATTAACCTCATCAACTCGGGGGAGATCGACCATGCAGATCTCCTAACGAGCATGGATCAACAGATATCAAGAGCAAAAGAAGAAGCTTCTAGCAGAATGACTATTATGGAAAAGGTGGAGAAGTGGGTGCTAGCACGTGATGAGGAGCGTTGGTTGGAAGAATATAGTAGAGACGAGAACCGGTACTCAGTCAGCAGAGGTGCACACAAGAACCTGAAACGTGCAGAACGTGCCCGAGTATTAGTCAACAAAATCCCAGCTTTGGTGGATTTGCTAATAGCAAATACTAAGAGTTGGGAAGAAGAAAGAAAGAAAACTTTCCTGTATGATGAGGTACCTCTCCTAGCAATGTTGGAAGAGTATAATGTGCTAAGGCAAGAAAAGGAGGAGGACAAGCAAAGACAACGGGAAATGAAGAAGGTCCAAAGCCAAGTAGTGGTCGAGCAAGAAAATTTGTTCATAACCAGGCCAAGCACCAGTACAAGGCGAACTTCAATCAGGAGTGTGAACGGAGGTTTTAGCAACGCCATGCCTCTAAACAGAAGGCTTTCACTGGGCCTTCAGCAATTGGGGCCACACAGCATAAACTCAGCCACTCAAGGCATCTCCTTTATAAAGGAAGGTAAAAAGGCACATAGACAAAAGTTGTTCGCTCGACCTGGCCTTGTTTCTCACCTTAGAGATGAAACAGCTTCGGTGGTATCAACATTTTCTGGCCCACAATCTCCCTGA

Protein Analysis

605

Amino Acids

69.22

Weight (kDa)

6.78

Isoelectric Point (pI)

49.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 34 - 525 1.2e-70 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1697
Acc36I ACCTGC 1 cut(s) 449
Acc65I GGTACC 1 cut(s) 1417
AccB1I GGYRCC 1 cut(s) 1417
AccB7I CCANNNNNTGG 2 cut(s) 247, 1519
AciI CCGC 2 cut(s) 90, 257
AclWI GGATC 4 cut(s) 831, 902, 975, 1146
AcoI YGGCCR 1 cut(s) 314
AcsI RAATTY 4 cut(s) 266, 432, 441, 1534
AcuI CTGAAG 4 cut(s) 86, 273, 972, 1628
AcvI CACGTG 1 cut(s) 1220
AcyI GRCGYC 2 cut(s) 47, 645
AfaI GTAC 6 cut(s) 423, 473, 539, 1268, 1419, 1564
AfiI CCNNNNNNNGG 4 cut(s) 247, 1345, 1519, 1566
AgeI ACCGGT 1 cut(s) 1263
AgsI TTSAA 4 cut(s) 146, 682, 1006, 1578
AjnI CCWGG 5 cut(s) 240, 307, 910, 1547, 1741
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
AloI GAACNNNNNNTCC 2 cut(s) 288, 320
AluBI AGCT 9 cut(s) 66, 230, 550, 678, 787, 1021, 1169, 1343, 1775
AluI AGCT 9 cut(s) 66, 230, 550, 678, 787, 1021, 1169, 1343, 1775
Alw21I GWGCWC 3 cut(s) 232, 1023, 1288
Alw26I GTCTC 3 cut(s) 306, 955, 1248
Alw44I GTGCAC 1 cut(s) 1284
AlwI GGATC 4 cut(s) 831, 902, 975, 1146
Ama87I CYCGRG 3 cut(s) 223, 1099, 1317
AoxI GGCC 6 cut(s) 314, 1550, 1639, 1655, 1744, 1798
ApaLI GTGCAC 1 cut(s) 1284
ApeKI GCWGC 1 cut(s) 160
ApoI RAATTY 4 cut(s) 266, 432, 441, 1534
AsiGI ACCGGT 1 cut(s) 1263
Asp700I GAANNNNTTC 4 cut(s) 410, 786, 1400, 1630
Asp718I GGTACC 1 cut(s) 1417
AspS9I GGNCC 4 cut(s) 1504, 1639, 1655, 1799
AsuHPI GGTGA 6 cut(s) 415, 510, 511, 652, 782, 1748
AsuNHI GCTAGC 1 cut(s) 1213
AvaI CYCGRG 3 cut(s) 223, 1099, 1317
AvaII GGWCC 1 cut(s) 1504
BaeGI GKGCMC 2 cut(s) 1288, 1318
BanI GGYRCC 1 cut(s) 1417
BanII GRGCYC 2 cut(s) 232, 1023
BbrPI CACGTG 1 cut(s) 1220
BbsI GAAGAC 1 cut(s) 483
Bbv12I GWGCWC 3 cut(s) 232, 1023, 1288
BbvCI CCTCAGC 1 cut(s) 945
BbvI GCAGC 1 cut(s) 147
BccI CCATC 3 cut(s) 25, 282, 801
BceAI ACGGC 1 cut(s) 329
BcgI CGANNNNNNTGC 2 cut(s) 40, 74
BciT130I CCWGG 5 cut(s) 242, 309, 912, 1549, 1743
BciVI GTATCC 1 cut(s) 86
BcoDI GTCTC 3 cut(s) 306, 955, 1248
BfaI CTAG 4 cut(s) 530, 1173, 1214, 1427
BfmI CTRYAG 1 cut(s) 447
BfuAI ACCTGC 1 cut(s) 449
BfuI GTATCC 1 cut(s) 86
BglII AGATCT 1 cut(s) 1120
BisI GCNGC 1 cut(s) 161
BlsI GCNGC 1 cut(s) 162
Bme1390I CCNGG 5 cut(s) 242, 309, 912, 1549, 1743
Bme18I GGWCC 1 cut(s) 1504
BmeT110I CYCGRG 3 cut(s) 223, 1099, 1317
BmgT120I GGNCC 4 cut(s) 1504, 1639, 1655, 1799
BmiI GGNNCC 2 cut(s) 1419, 1656
BmrFI CCNGG 5 cut(s) 242, 309, 912, 1549, 1743
BmrI ACTGGG 1 cut(s) 1646
BmsI GCATC 2 cut(s) 598, 1695
BmtI GCTAGC 1 cut(s) 1217
BmuI ACTGGG 1 cut(s) 1646
BpiI GAAGAC 1 cut(s) 483
BpmI CTGGAG 1 cut(s) 206
Bpu10I CCTNAGC 2 cut(s) 945, 1454
BpuEI CTTGAG 1 cut(s) 1665
BsaAI YACGTR 1 cut(s) 1220
BsaHI GRCGYC 2 cut(s) 47, 645
BsaI GGTCTC 1 cut(s) 306
BsaJI CCNNGG 4 cut(s) 289, 307, 663, 754
BsaWI WCCGGW 1 cut(s) 1263
BsaXI ACNNNNNCTCC 8 cut(s) 164, 194, 288, 318, 543, 573, 1220, 1250
Bsc4I CCNNNNNNNGG 4 cut(s) 247, 1345, 1519, 1566
Bse118I RCCGGY 2 cut(s) 364, 1263
Bse1I ACTGG 4 cut(s) 877, 969, 1560, 1641
Bse3DI GCAATG 1 cut(s) 1437
BseBI CCWGG 5 cut(s) 242, 309, 912, 1549, 1743
BseDI CCNNGG 4 cut(s) 289, 307, 663, 754
BseGI GGATG 1 cut(s) 670
BseLI CCNNNNNNNGG 4 cut(s) 247, 1345, 1519, 1566
BseMI GCAATG 1 cut(s) 1437
BseMII CTCAG 3 cut(s) 936, 1284, 1686
BseNI ACTGG 4 cut(s) 877, 969, 1560, 1641
BseRI GAGGAG 3 cut(s) 548, 1241, 1484
BseSI GKGCMC 2 cut(s) 1288, 1318
BseXI GCAGC 1 cut(s) 147
BseYI CCCAGC 1 cut(s) 1339
BsgI GTGCAG 2 cut(s) 221, 1326
BshFI GGCC 6 cut(s) 316, 1552, 1641, 1657, 1746, 1800
BshNI GGYRCC 1 cut(s) 1417
BshTI ACCGGT 1 cut(s) 1263
BsiHKAI GWGCWC 3 cut(s) 232, 1023, 1288
BsiHKCI CYCGRG 3 cut(s) 223, 1099, 1317
BsiSI CCGG 2 cut(s) 365, 1264
BslFI GGGAC 1 cut(s) 271
BslI CCNNNNNNNGG 4 cut(s) 247, 1345, 1519, 1566
BsmAI GTCTC 3 cut(s) 306, 955, 1248
BsmBI CGTCTC 1 cut(s) 1248
BsmFI GGGAC 1 cut(s) 271
BsmI GAATGC 1 cut(s) 63
BsnI GGCC 6 cut(s) 316, 1552, 1641, 1657, 1746, 1800
Bso31I GGTCTC 1 cut(s) 306
BsoBI CYCGRG 3 cut(s) 223, 1099, 1317
Bsp1286I GDGCHC 4 cut(s) 232, 1023, 1288, 1318
Bsp143I GATC 9 cut(s) 136, 511, 823, 847, 907, 967, 1107, 1120, 1138
BspACI CCGC 2 cut(s) 90, 257
BspANI GGCC 6 cut(s) 316, 1552, 1641, 1657, 1746, 1800
BspCNI CTCAG 3 cut(s) 937, 1283, 1685
BspHI TCATGA 1 cut(s) 585
BspLI GGNNCC 2 cut(s) 1419, 1656
BspMAI CTGCAG 1 cut(s) 451
BspMI ACCTGC 1 cut(s) 449
BspOI GCTAGC 1 cut(s) 1217
BspPI GGATC 4 cut(s) 831, 902, 975, 1146
BspT107I GGYRCC 1 cut(s) 1417
BspTNI GGTCTC 1 cut(s) 306
BsrDI GCAATG 1 cut(s) 1437
BsrFI RCCGGY 2 cut(s) 364, 1263
BsrI ACTGG 4 cut(s) 877, 969, 1560, 1641
BssAI RCCGGY 2 cut(s) 364, 1263
BssECI CCNNGG 4 cut(s) 289, 307, 663, 754
BssMI GATC 9 cut(s) 136, 511, 823, 847, 907, 967, 1107, 1120, 1138
BssNI GRCGYC 2 cut(s) 47, 645
BssT1I CCWWGG 3 cut(s) 289, 663, 754
Bst2UI CCWGG 5 cut(s) 242, 309, 912, 1549, 1743
Bst4CI ACNGT 4 cut(s) 300, 348, 719, 745
Bst6I CTCTTC 1 cut(s) 1434
BstACI GRCGYC 2 cut(s) 47, 645
BstAPI GCANNNNNTGC 1 cut(s) 1313
BstBAI YACGTR 1 cut(s) 1220
BstC8I GCNNGC 3 cut(s) 366, 982, 1215
BstDEI CTNAG 6 cut(s) 945, 1270, 1371, 1454, 1672, 1760
BstF5I GGATG 1 cut(s) 670
BstKTI GATC 9 cut(s) 139, 514, 826, 850, 910, 970, 1110, 1123, 1141
BstMAI GTCTC 3 cut(s) 306, 955, 1248
BstMBI GATC 9 cut(s) 136, 511, 823, 847, 907, 967, 1107, 1120, 1138
BstMWI GCNNNNNNNGC 3 cut(s) 455, 1313, 1654
BstNI CCWGG 5 cut(s) 242, 309, 912, 1549, 1743
BstSCI CCNGG 5 cut(s) 240, 307, 910, 1547, 1741
BstSFI CTRYAG 1 cut(s) 447
BstSLI GKGCMC 2 cut(s) 1288, 1318
BstV1I GCAGC 1 cut(s) 147
BstV2I GAAGAC 1 cut(s) 483
BstX2I RGATCY 3 cut(s) 823, 907, 1120
BstXI CCANNNNNNTGG 2 cut(s) 76, 872
BstYI RGATCY 3 cut(s) 823, 907, 1120
BsuI GTATCC 1 cut(s) 86
BsuRI GGCC 6 cut(s) 316, 1552, 1641, 1657, 1746, 1800
BtsCI GGATG 1 cut(s) 670
BtsIMutI CAGTG 3 cut(s) 335, 750, 1634
BveI ACCTGC 1 cut(s) 449
Cac8I GCNNGC 3 cut(s) 366, 982, 1215
CciI TCATGA 1 cut(s) 585
Cfr10I RCCGGY 2 cut(s) 364, 1263
Cfr13I GGNCC 4 cut(s) 1504, 1639, 1655, 1799
CseI GACGC 2 cut(s) 55, 653
Csp6I GTAC 6 cut(s) 422, 472, 538, 1267, 1418, 1563
CspAI ACCGGT 1 cut(s) 1263
CviAII CATG 8 cut(s) 496, 586, 829, 866, 1048, 1115, 1135, 1612
CviQI GTAC 6 cut(s) 422, 472, 538, 1267, 1418, 1563
DdeI CTNAG 6 cut(s) 945, 1270, 1371, 1454, 1672, 1760
DpnI GATC 9 cut(s) 138, 513, 825, 849, 909, 969, 1109, 1122, 1140
DpnII GATC 9 cut(s) 136, 511, 823, 847, 907, 967, 1107, 1120, 1138
EaeI YGGCCR 1 cut(s) 314
Eam1104I CTCTTC 1 cut(s) 1434
EarI CTCTTC 1 cut(s) 1434
Ecl136II GAGCTC 2 cut(s) 230, 1021
Eco130I CCWWGG 3 cut(s) 289, 663, 754
Eco24I GRGCYC 2 cut(s) 232, 1023
Eco31I GGTCTC 1 cut(s) 306
Eco32I GATATC 1 cut(s) 1149
Eco47I GGWCC 1 cut(s) 1504
Eco53kI GAGCTC 2 cut(s) 230, 1021
Eco57I CTGAAG 4 cut(s) 86, 273, 972, 1628
Eco72I CACGTG 1 cut(s) 1220
Eco88I CYCGRG 3 cut(s) 223, 1099, 1317
EcoICRI GAGCTC 2 cut(s) 230, 1021
EcoRII CCWGG 5 cut(s) 240, 307, 910, 1547, 1741
EcoRV GATATC 1 cut(s) 1149
EcoT14I CCWWGG 3 cut(s) 289, 663, 754
EcoT38I GRGCYC 2 cut(s) 232, 1023
ErhI CCWWGG 3 cut(s) 289, 663, 754
Esp3I CGTCTC 1 cut(s) 1248
FaeI CATG 8 cut(s) 499, 589, 832, 869, 1051, 1118, 1138, 1615
FalI AAGNNNNNCTT 2 cut(s) 1558, 1590
FaqI GGGAC 1 cut(s) 271
FatI CATG 8 cut(s) 495, 585, 828, 865, 1047, 1114, 1134, 1611
Fnu4HI GCNGC 1 cut(s) 161
FokI GGATG 1 cut(s) 657
FriOI GRGCYC 2 cut(s) 232, 1023
Fsp4HI GCNGC 1 cut(s) 161
FspBI CTAG 4 cut(s) 530, 1173, 1214, 1427
GluI GCNGC 1 cut(s) 161
GsaI CCCAGC 1 cut(s) 1343
GsuI CTGGAG 1 cut(s) 206
HaeIII GGCC 6 cut(s) 316, 1552, 1641, 1657, 1746, 1800
HapII CCGG 2 cut(s) 365, 1264
HgaI GACGC 2 cut(s) 55, 653
Hin1I GRCGYC 2 cut(s) 47, 645
Hin1II CATG 8 cut(s) 499, 589, 832, 869, 1051, 1118, 1138, 1615
HincII GTYRAC 1 cut(s) 1330
HindII GTYRAC 1 cut(s) 1330
HindIII AAGCTT 3 cut(s) 676, 785, 1167
HinfI GANTC 5 cut(s) 353, 558, 749, 777, 1054
HpaII CCGG 2 cut(s) 365, 1264
HphI GGTGA 6 cut(s) 415, 510, 511, 652, 782, 1748
Hpy166II GTNNAC 4 cut(s) 616, 1286, 1330, 1591
Hpy188I TCNGA 7 cut(s) 30, 45, 416, 438, 469, 907, 1069
Hpy188III TCNNGA 8 cut(s) 104, 185, 225, 586, 958, 1006, 1152, 1582
Hpy8I GTNNAC 4 cut(s) 616, 1286, 1330, 1591
HpyAV CCTTC 7 cut(s) 148, 196, 1068, 1495, 1620, 1652, 1697
HpyCH4III ACNGT 4 cut(s) 300, 348, 719, 745
HpyCH4IV ACGT 3 cut(s) 1219, 1303, 1312
HpyCH4V TGCA 7 cut(s) 112, 238, 449, 611, 1118, 1286, 1307
HpyF10VI GCNNNNNNNGC 3 cut(s) 455, 1313, 1654
HpyF3I CTNAG 6 cut(s) 945, 1270, 1371, 1454, 1672, 1760
HpySE526I ACGT 3 cut(s) 1219, 1303, 1312
Hsp92I GRCGYC 2 cut(s) 47, 645
Hsp92II CATG 8 cut(s) 499, 589, 832, 869, 1051, 1118, 1138, 1615
KpnI GGTACC 1 cut(s) 1421
KroI GCCGGC 1 cut(s) 364
KroNI GCCGGC 1 cut(s) 366
Kzo9I GATC 9 cut(s) 136, 511, 823, 847, 907, 967, 1107, 1120, 1138
LmnI GCTCC 3 cut(s) 71, 172, 1228
Lsp1109I GCAGC 1 cut(s) 147
LweI GCATC 2 cut(s) 598, 1695
MaeI CTAG 4 cut(s) 530, 1173, 1214, 1427
MaeII ACGT 3 cut(s) 1219, 1303, 1312
MaeIII GTNAC 1 cut(s) 868
MalI GATC 9 cut(s) 138, 513, 825, 849, 909, 969, 1109, 1122, 1140
MboI GATC 9 cut(s) 136, 511, 823, 847, 907, 967, 1107, 1120, 1138
MfeI CAATTG 1 cut(s) 1649
MflI RGATCY 3 cut(s) 823, 907, 1120
MhlI GDGCHC 4 cut(s) 232, 1023, 1288, 1318
MlyI GAGTC 2 cut(s) 552, 771
MmeI TCCRAC 4 cut(s) 109, 330, 1218, 1416
MroNI GCCGGC 1 cut(s) 364
MroXI GAANNNNTTC 4 cut(s) 410, 786, 1400, 1630
MseI TTAA 3 cut(s) 735, 974, 1086
MslI CAYNNNNRTG 1 cut(s) 29
MspI CCGG 2 cut(s) 365, 1264
MspR9I CCNGG 5 cut(s) 242, 309, 912, 1549, 1743
MunI CAATTG 1 cut(s) 1649
Mva1269I GAATGC 1 cut(s) 63
MvaI CCWGG 5 cut(s) 242, 309, 912, 1549, 1743
MwoI GCNNNNNNNGC 3 cut(s) 455, 1313, 1654
NaeI GCCGGC 1 cut(s) 366
NdeII GATC 9 cut(s) 136, 511, 823, 847, 907, 967, 1107, 1120, 1138
NgoMIV GCCGGC 1 cut(s) 364
NheI GCTAGC 1 cut(s) 1213
NlaIII CATG 8 cut(s) 499, 589, 832, 869, 1051, 1118, 1138, 1615
NlaIV GGNNCC 2 cut(s) 1419, 1656
PaeR7I CTCGAG 1 cut(s) 223
PagI TCATGA 1 cut(s) 585
PcsI WCGNNNNNNNCGW 1 cut(s) 1309
PctI GAATGC 1 cut(s) 63
PdiI GCCGGC 1 cut(s) 366
PdmI GAANNNNTTC 4 cut(s) 410, 786, 1400, 1630
PfeI GAWTC 3 cut(s) 353, 749, 1054
PflMI CCANNNNNTGG 2 cut(s) 247, 1519
PfoI TCCNGGA 1 cut(s) 910
PinAI ACCGGT 1 cut(s) 1263
PkrI GCNGC 1 cut(s) 162
PleI GAGTC 2 cut(s) 552, 771
PmaCI CACGTG 1 cut(s) 1220
PmlI CACGTG 1 cut(s) 1220
PpsI GAGTC 2 cut(s) 552, 771
Ppu21I YACGTR 1 cut(s) 1220
PsiI TTATAA 1 cut(s) 1697
Psp124BI GAGCTC 2 cut(s) 232, 1023
Psp6I CCWGG 5 cut(s) 240, 307, 910, 1547, 1741
PspCI CACGTG 1 cut(s) 1220
PspFI CCCAGC 1 cut(s) 1339
PspGI CCWGG 5 cut(s) 240, 307, 910, 1547, 1741
PspN4I GGNNCC 2 cut(s) 1419, 1656
PspPI GGNCC 4 cut(s) 1504, 1639, 1655, 1799
PstI CTGCAG 1 cut(s) 451
PsuI RGATCY 3 cut(s) 823, 907, 1120
RsaI GTAC 6 cut(s) 423, 473, 539, 1268, 1419, 1564
RsaNI GTAC 6 cut(s) 422, 472, 538, 1267, 1418, 1563
RseI CAYNNNNRTG 1 cut(s) 29
SacI GAGCTC 2 cut(s) 232, 1023
SaqAI TTAA 3 cut(s) 735, 974, 1086
SatI GCNGC 1 cut(s) 161
Sau3AI GATC 9 cut(s) 136, 511, 823, 847, 907, 967, 1107, 1120, 1138
Sau96I GGNCC 4 cut(s) 1504, 1639, 1655, 1799
SchI GAGTC 2 cut(s) 552, 771
ScrFI CCNGG 5 cut(s) 242, 309, 912, 1549, 1743
SduI GDGCHC 4 cut(s) 232, 1023, 1288, 1318
SfaNI GCATC 2 cut(s) 598, 1695
SfcI CTRYAG 1 cut(s) 447
Sfr274I CTCGAG 1 cut(s) 223
SinI GGWCC 1 cut(s) 1504
SlaI CTCGAG 1 cut(s) 223
SmiMI CAYNNNNRTG 1 cut(s) 29
SmlI CTYRAG 2 cut(s) 223, 1680
SmoI CTYRAG 2 cut(s) 223, 1680
SsiI CCGC 2 cut(s) 90, 257
SspMI CTAG 4 cut(s) 530, 1173, 1214, 1427
SstI GAGCTC 2 cut(s) 232, 1023
StyD4I CCNGG 5 cut(s) 240, 307, 910, 1547, 1741
StyI CCWWGG 3 cut(s) 289, 663, 754
TaaI ACNGT 4 cut(s) 300, 348, 719, 745
TaiI ACGT 3 cut(s) 1222, 1306, 1315
TaqI TCGA 8 cut(s) 224, 232, 488, 594, 780, 1110, 1524, 1738
TatI WGTACW 2 cut(s) 421, 1562
TfiI GAWTC 3 cut(s) 353, 749, 1054
Tru1I TTAA 3 cut(s) 735, 974, 1086
Tru9I TTAA 3 cut(s) 735, 974, 1086
TscAI CASTG 3 cut(s) 342, 750, 1641
TseI GCWGC 1 cut(s) 160
TspDTI ATGAA 9 cut(s) 138, 591, 659, 699, 879, 1004, 1511, 1531, 1782
TspGWI ACGGA 1 cut(s) 1608
TspRI CASTG 3 cut(s) 342, 750, 1641
Van91I CCANNNNNTGG 2 cut(s) 247, 1519
VneI GTGCAC 1 cut(s) 1284
VpaK11BI GGWCC 1 cut(s) 1504
XapI RAATTY 4 cut(s) 266, 432, 441, 1534
XhoI CTCGAG 1 cut(s) 223
XmnI GAANNNNTTC 4 cut(s) 410, 786, 1400, 1630
XspI CTAG 4 cut(s) 530, 1173, 1214, 1427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.