Rh4BG306100

65-kDa microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0007124
Physical Location & Seq
Reverse (-)
47609535 .. 47617682
8148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG306100.1

Sequence Viewer

Length: 1353 bp
ATGGGTTCATTTCGAGCACCAGTGGGGAGAAGCTCTGCACTTCCTGAATCATCATGTGGATATTTGCTTCAAGAACTACAGATGATTTGGAATGAAATTGGACAAGATCAGTTGGAAAGGGAGAAGGTACTGGTGGATTTAGAGCAGGAATGCCTGGAGGTCTATAGGAGGAAGGTTGATGCTGCTAATACCTCAAGAGCTCGCCTGCACCAGGAGTTGGCAGAAGCTGAGGCTGAATTTACTCATCTTCTTTTGTCACTTGGCGAACGGTCTCTCCCCGGACGGCCAGAAAAAATGGTGGGAACACTGAAAGAGCAGCTAGATTCGATCACGCCAGCTCTGCGAGACATGAGATTGAGGAAAGAAGATAGGGTGAACAAGTTCCGAGCTGTACAAGGGCAAATTCAGAAGATCTCTGCAGAAATAGCAGGACAGTCAGAGTATGAGAACTCATCATCAGATGTCAGGGTGAATGAGAATGATCTTTCCTTGAAGAAACTAGAGGAGTACCAGACGGAGCTACAAAGACTCCACAATGACAGGAATGAAAGGCTCAAGCGGGTGGAGAAATATATAAACTCCATCCAAAAGTTGTCTTCAATATTAGGAACAGACTCCTCTATGATCATCACCAAGGTTCACCCAAGCTTGAATGAGTTATGTGGGATAGCCAAGAACATAAGCAACAGTATTTTGGCTAAACTCAACAGCACAGTGGAATCCCTAGAGGAAGAAAAGCAAAAGCGGCTTGAAAAGCTTCACCATCTTGGTAAAGCATTGACAAACTTATGGAATCTCATGGATACACCATATAAAGATCGTCAATTATTTTCCCATGTTACCGGCTTATTATCAGTCTCATCAGCAGAAGTATCAGATCCAGGAAGCCTAACCCAAAAGATAATCCAGCAGGCTGAGGCTGAAGTCAAGAGACTGGATCAATTAAAAGTAAGCAAGATGAAAGAACTATTTCTCAAGAAACAGAATGAGCTCAAGGAGATATGCAAAAAATCACACATGGAGATTCCTGTGCAAGCAAAGATGGATAATATAATTAACCTCATGAAATCTGGGGAAATTGACCATGCAGATCTTGTTATGAGCATGGATCAACAGATATCAAGAGCAGAAGATGAAGCTTGTAGCAGAAAGACAATTATGGAAAAGGTGGAAAAGTGGATCCTAGCACGTGATGAGGAGCGCTGGTTGGAAGAATATGGAAGAGACGAAAACCGATACTCAGTCAGAAGAGGTGCACACAAGAACCTGAGACGTGCAGAACGTGCTCGAGTATTAGTTAGCAAAATTCCAGGTAATATTGAGATACCTGTACGCATTCATACCCAACAGTAA

Protein Analysis

450

Amino Acids

51.74

Weight (kDa)

7.67

Isoelectric Point (pI)

51.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 22 - 438 9.5e-59 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 217
AciI CCGC 2 cut(s) 559, 745
AclWI GGATC 5 cut(s) 872, 945, 1116, 1174, 1187
AcoI YGGCCR 1 cut(s) 284
AcsI RAATTY 3 cut(s) 236, 402, 1305
AcuI CTGAAG 1 cut(s) 942
AcvI CACGTG 1 cut(s) 1190
AfaI GTAC 4 cut(s) 129, 393, 509, 1332
AfeI AGCGCT 1 cut(s) 1202
AfiI CCNNNNNNNGG 2 cut(s) 211, 217
AgsI TTSAA 5 cut(s) 71, 493, 600, 652, 752
AjiI CACGTC 1 cut(s) 1274
AjnI CCWGG 4 cut(s) 153, 210, 880, 1309
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
Alw21I GWGCWC 5 cut(s) 19, 202, 993, 1258, 1288
Alw26I GTCTC 6 cut(s) 276, 339, 862, 925, 1218, 1264
Alw44I GTGCAC 1 cut(s) 1254
AlwI GGATC 5 cut(s) 872, 945, 1116, 1174, 1187
AlwNI CAGNNNCTG 1 cut(s) 227
Ama87I CYCGRG 1 cut(s) 1287
Aor51HI AGCGCT 1 cut(s) 1202
AoxI GGCC 1 cut(s) 284
ApaLI GTGCAC 1 cut(s) 1254
ApeKI GCWGC 2 cut(s) 182, 316
ApoI RAATTY 3 cut(s) 236, 402, 1305
Asp700I GAANNNNTTC 3 cut(s) 380, 756, 969
AspLEI GCGC 1 cut(s) 1203
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 5 cut(s) 385, 481, 622, 632, 752
AvaI CYCGRG 1 cut(s) 1287
BaeGI GKGCMC 1 cut(s) 1258
BamHI GGATCC 1 cut(s) 1179
BanII GRGCYC 2 cut(s) 202, 993
BbrPI CACGTG 1 cut(s) 1190
BbsI GAAGAC 1 cut(s) 588
Bbv12I GWGCWC 5 cut(s) 19, 202, 993, 1258, 1288
BbvCI CCTCAGC 2 cut(s) 228, 915
BbvI GCAGC 2 cut(s) 169, 328
BccI CCATC 3 cut(s) 590, 771, 1036
BceAI ACGGC 1 cut(s) 299
BciT130I CCWGG 4 cut(s) 155, 212, 882, 1311
BciVI GTATCC 1 cut(s) 796
BclI TGATCA 1 cut(s) 624
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 6 cut(s) 276, 339, 862, 925, 1218, 1264
BfaI CTAG 4 cut(s) 320, 500, 725, 1184
BfmI CTRYAG 3 cut(s) 77, 163, 417
BfoI RGCGCY 1 cut(s) 1204
BfuI GTATCC 1 cut(s) 796
BglII AGATCT 2 cut(s) 411, 1090
BisI GCNGC 3 cut(s) 183, 317, 746
BlsI GCNGC 3 cut(s) 184, 318, 747
Bme1390I CCNGG 5 cut(s) 155, 212, 279, 882, 1311
BmeT110I CYCGRG 1 cut(s) 1287
BmgBI CACGTC 1 cut(s) 1274
BmiI GGNNCC 1 cut(s) 1181
BmrFI CCNGG 5 cut(s) 155, 212, 279, 882, 1311
BmsI GCATC 1 cut(s) 169
BpiI GAAGAC 1 cut(s) 588
BpmI CTGGAG 1 cut(s) 176
Bpu10I CCTNAGC 2 cut(s) 228, 915
BpuEI CTTGAG 4 cut(s) 178, 539, 959, 977
BpuMI CCSGG 1 cut(s) 279
BsaAI YACGTR 1 cut(s) 1190
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 2 cut(s) 277, 633
BsaXI ACNNNNNCTCC 6 cut(s) 258, 288, 513, 543, 1190, 1220
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 217
Bse118I RCCGGY 1 cut(s) 842
Bse1I ACTGG 3 cut(s) 20, 135, 939
BseBI CCWGG 4 cut(s) 155, 212, 882, 1311
BseDI CCNNGG 2 cut(s) 277, 633
BseGI GGATG 1 cut(s) 582
BseLI CCNNNNNNNGG 2 cut(s) 211, 217
BseMII CTCAG 4 cut(s) 219, 906, 1254, 1259
BseNI ACTGG 3 cut(s) 20, 135, 939
BseRI GAGGAG 3 cut(s) 518, 607, 1211
BseSI GKGCMC 1 cut(s) 1258
BseXI GCAGC 2 cut(s) 169, 328
BsgI GTGCAG 3 cut(s) 21, 191, 1296
BshFI GGCC 1 cut(s) 286
BsiHKAI GWGCWC 5 cut(s) 19, 202, 993, 1258, 1288
BsiHKCI CYCGRG 1 cut(s) 1287
BsiSI CCGG 2 cut(s) 279, 843
BslI CCNNNNNNNGG 2 cut(s) 211, 217
BsmAI GTCTC 6 cut(s) 276, 339, 862, 925, 1218, 1264
BsmBI CGTCTC 2 cut(s) 1218, 1264
BsmI GAATGC 2 cut(s) 155, 1335
BsnI GGCC 1 cut(s) 286
Bso31I GGTCTC 1 cut(s) 276
BsoBI CYCGRG 1 cut(s) 1287
Bsp1286I GDGCHC 5 cut(s) 19, 202, 993, 1258, 1288
Bsp1407I TGTACA 1 cut(s) 391
BspACI CCGC 2 cut(s) 559, 745
BspANI GGCC 1 cut(s) 286
BspCNI CTCAG 4 cut(s) 220, 907, 1253, 1260
BspHI TCATGA 1 cut(s) 1062
BspLI GGNNCC 1 cut(s) 1181
BspMAI CTGCAG 1 cut(s) 421
BspPI GGATC 5 cut(s) 872, 945, 1116, 1174, 1187
BspTNI GGTCTC 1 cut(s) 276
BsrFI RCCGGY 1 cut(s) 842
BsrGI TGTACA 1 cut(s) 391
BsrI ACTGG 3 cut(s) 20, 135, 939
BssAI RCCGGY 1 cut(s) 842
BssECI CCNNGG 2 cut(s) 277, 633
BssT1I CCWWGG 1 cut(s) 633
Bst2UI CCWGG 4 cut(s) 155, 212, 882, 1311
Bst4CI ACNGT 5 cut(s) 270, 435, 689, 715, 1350
Bst6I CTCTTC 2 cut(s) 1216, 1243
BstAPI GCANNNNNTGC 1 cut(s) 1283
BstAUI TGTACA 1 cut(s) 391
BstBAI YACGTR 1 cut(s) 1190
BstC8I GCNNGC 5 cut(s) 202, 206, 336, 912, 1035
BstDEI CTNAG 4 cut(s) 228, 915, 1240, 1268
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 1 cut(s) 582
BstH2I RGCGCY 1 cut(s) 1204
BstHHI GCGC 1 cut(s) 1203
BstMAI GTCTC 6 cut(s) 276, 339, 862, 925, 1218, 1264
BstMWI GCNNNNNNNGC 5 cut(s) 340, 425, 745, 754, 1283
BstNI CCWGG 4 cut(s) 155, 212, 882, 1311
BstSCI CCNGG 5 cut(s) 153, 210, 277, 880, 1309
BstSFI CTRYAG 3 cut(s) 77, 163, 417
BstSLI GKGCMC 1 cut(s) 1258
BstV1I GCAGC 2 cut(s) 169, 328
BstV2I GAAGAC 1 cut(s) 588
BstX2I RGATCY 4 cut(s) 411, 877, 1090, 1179
BstYI RGATCY 4 cut(s) 411, 877, 1090, 1179
BsuI GTATCC 1 cut(s) 796
BsuRI GGCC 1 cut(s) 286
BtrI CACGTC 1 cut(s) 1274
BtsCI GGATG 1 cut(s) 582
BtsIMutI CAGTG 3 cut(s) 27, 305, 720
Cac8I GCNNGC 5 cut(s) 202, 206, 336, 912, 1035
CaiI CAGNNNCTG 1 cut(s) 227
CciI TCATGA 1 cut(s) 1062
CfoI GCGC 1 cut(s) 1203
Cfr10I RCCGGY 1 cut(s) 842
Csp6I GTAC 4 cut(s) 128, 392, 508, 1331
CviAII CATG 8 cut(s) 54, 349, 799, 836, 1018, 1063, 1085, 1105
CviQI GTAC 4 cut(s) 128, 392, 508, 1331
DdeI CTNAG 4 cut(s) 228, 915, 1240, 1268
EaeI YGGCCR 1 cut(s) 284
Eam1104I CTCTTC 2 cut(s) 1216, 1243
EarI CTCTTC 2 cut(s) 1216, 1243
Ecl136II GAGCTC 2 cut(s) 200, 991
Eco130I CCWWGG 1 cut(s) 633
Eco24I GRGCYC 2 cut(s) 202, 993
Eco31I GGTCTC 1 cut(s) 276
Eco32I GATATC 1 cut(s) 1119
Eco47III AGCGCT 1 cut(s) 1202
Eco53kI GAGCTC 2 cut(s) 200, 991
Eco57I CTGAAG 1 cut(s) 942
Eco72I CACGTG 1 cut(s) 1190
Eco88I CYCGRG 1 cut(s) 1287
EcoICRI GAGCTC 2 cut(s) 200, 991
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoRII CCWGG 4 cut(s) 153, 210, 880, 1309
EcoRV GATATC 1 cut(s) 1119
EcoT14I CCWWGG 1 cut(s) 633
EcoT38I GRGCYC 2 cut(s) 202, 993
ErhI CCWWGG 1 cut(s) 633
Esp3I CGTCTC 2 cut(s) 1218, 1264
FaeI CATG 8 cut(s) 57, 352, 802, 839, 1021, 1066, 1088, 1108
FatI CATG 8 cut(s) 53, 348, 798, 835, 1017, 1062, 1084, 1104
FauI CCCGC 1 cut(s) 552
FbaI TGATCA 1 cut(s) 624
Fnu4HI GCNGC 3 cut(s) 183, 317, 746
FokI GGATG 1 cut(s) 569
FriOI GRGCYC 2 cut(s) 202, 993
Fsp4HI GCNGC 3 cut(s) 183, 317, 746
FspBI CTAG 4 cut(s) 320, 500, 725, 1184
GlaI GCGC 1 cut(s) 1202
GluI GCNGC 3 cut(s) 183, 317, 746
GsuI CTGGAG 1 cut(s) 176
HaeII RGCGCY 1 cut(s) 1204
HaeIII GGCC 1 cut(s) 286
HapII CCGG 2 cut(s) 279, 843
HhaI GCGC 1 cut(s) 1203
Hin1II CATG 8 cut(s) 57, 352, 802, 839, 1021, 1066, 1088, 1108
Hin6I GCGC 1 cut(s) 1201
HinP1I GCGC 1 cut(s) 1201
HindIII AAGCTT 3 cut(s) 646, 755, 1137
HinfI GANTC 7 cut(s) 47, 323, 528, 614, 719, 793, 1024
HpaII CCGG 2 cut(s) 279, 843
HphI GGTGA 5 cut(s) 385, 481, 622, 632, 752
Hpy166II GTNNAC 3 cut(s) 376, 640, 1256
Hpy188I TCNGA 6 cut(s) 386, 408, 439, 460, 877, 1247
Hpy188III TCNNGA 7 cut(s) 44, 71, 195, 928, 976, 1063, 1122
Hpy8I GTNNAC 3 cut(s) 376, 640, 1256
HpyAV CCTTC 2 cut(s) 118, 166
HpyCH4III ACNGT 5 cut(s) 270, 435, 689, 715, 1350
HpyCH4IV ACGT 3 cut(s) 1189, 1273, 1282
HpyCH4V TGCA 8 cut(s) 38, 208, 419, 1005, 1033, 1088, 1256, 1277
HpyF10VI GCNNNNNNNGC 5 cut(s) 340, 425, 745, 754, 1283
HpyF3I CTNAG 4 cut(s) 228, 915, 1240, 1268
HpySE526I ACGT 3 cut(s) 1189, 1273, 1282
Hsp92II CATG 8 cut(s) 57, 352, 802, 839, 1021, 1066, 1088, 1108
HspAI GCGC 1 cut(s) 1201
Ksp22I TGATCA 1 cut(s) 624
LmnI GCTCC 2 cut(s) 517, 1198
Lsp1109I GCAGC 2 cut(s) 169, 328
LweI GCATC 1 cut(s) 169
MaeI CTAG 4 cut(s) 320, 500, 725, 1184
MaeII ACGT 3 cut(s) 1189, 1273, 1282
MaeIII GTNAC 2 cut(s) 255, 838
MflI RGATCY 4 cut(s) 411, 877, 1090, 1179
MhlI GDGCHC 5 cut(s) 19, 202, 993, 1258, 1288
MluCI AATT 9 cut(s) 96, 236, 402, 824, 941, 1053, 1077, 1155, 1305
MlyI GAGTC 2 cut(s) 522, 608
MmeI TCCRAC 2 cut(s) 93, 1188
MroXI GAANNNNTTC 3 cut(s) 380, 756, 969
MseI TTAA 2 cut(s) 944, 1056
MspI CCGG 2 cut(s) 279, 843
MspR9I CCNGG 5 cut(s) 155, 212, 279, 882, 1311
Mva1269I GAATGC 2 cut(s) 155, 1335
MvaI CCWGG 4 cut(s) 155, 212, 882, 1311
MwoI GCNNNNNNNGC 5 cut(s) 340, 425, 745, 754, 1283
NciI CCSGG 1 cut(s) 279
NlaIII CATG 8 cut(s) 57, 352, 802, 839, 1021, 1066, 1088, 1108
NlaIV GGNNCC 1 cut(s) 1181
NmuCI GTSAC 1 cut(s) 255
PaeR7I CTCGAG 1 cut(s) 1287
PagI TCATGA 1 cut(s) 1062
PcsI WCGNNNNNNNCGW 1 cut(s) 1279
PctI GAATGC 2 cut(s) 155, 1335
PdmI GAANNNNTTC 3 cut(s) 380, 756, 969
PfeI GAWTC 5 cut(s) 47, 323, 719, 793, 1024
PflMI CCANNNNNTGG 1 cut(s) 217
PfoI TCCNGGA 1 cut(s) 880
PkrI GCNGC 3 cut(s) 184, 318, 747
PleI GAGTC 2 cut(s) 522, 608
PmaCI CACGTG 1 cut(s) 1190
PmlI CACGTG 1 cut(s) 1190
PpsI GAGTC 2 cut(s) 522, 608
Ppu21I YACGTR 1 cut(s) 1190
Psp124BI GAGCTC 2 cut(s) 202, 993
Psp6I CCWGG 4 cut(s) 153, 210, 880, 1309
PspCI CACGTG 1 cut(s) 1190
PspGI CCWGG 4 cut(s) 153, 210, 880, 1309
PspN4I GGNNCC 1 cut(s) 1181
PspXI VCTCGAGB 1 cut(s) 1287
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 227
PsuI RGATCY 4 cut(s) 411, 877, 1090, 1179
RsaI GTAC 4 cut(s) 129, 393, 509, 1332
RsaNI GTAC 4 cut(s) 128, 392, 508, 1331
SacI GAGCTC 2 cut(s) 202, 993
SaqAI TTAA 2 cut(s) 944, 1056
SatI GCNGC 3 cut(s) 183, 317, 746
SchI GAGTC 2 cut(s) 522, 608
ScrFI CCNGG 5 cut(s) 155, 212, 279, 882, 1311
SduI GDGCHC 5 cut(s) 19, 202, 993, 1258, 1288
SfaNI GCATC 1 cut(s) 169
SfcI CTRYAG 3 cut(s) 77, 163, 417
Sfr274I CTCGAG 1 cut(s) 1287
SlaI CTCGAG 1 cut(s) 1287
SmlI CTYRAG 5 cut(s) 193, 554, 974, 992, 1287
SmoI CTYRAG 5 cut(s) 193, 554, 974, 992, 1287
Sse9I AATT 9 cut(s) 96, 236, 402, 824, 941, 1053, 1077, 1155, 1305
SsiI CCGC 2 cut(s) 559, 745
SspI AATATT 2 cut(s) 603, 1318
SspMI CTAG 4 cut(s) 320, 500, 725, 1184
SstI GAGCTC 2 cut(s) 202, 993
StyD4I CCNGG 5 cut(s) 153, 210, 277, 880, 1309
StyI CCWWGG 1 cut(s) 633
TaaI ACNGT 5 cut(s) 270, 435, 689, 715, 1350
TaiI ACGT 3 cut(s) 1192, 1276, 1285
TaqI TCGA 3 cut(s) 13, 326, 1288
TasI AATT 9 cut(s) 96, 236, 402, 824, 941, 1053, 1077, 1155, 1305
TatI WGTACW 1 cut(s) 391
TauI GCSGC 1 cut(s) 748
TfiI GAWTC 5 cut(s) 47, 323, 719, 793, 1024
Tru1I TTAA 2 cut(s) 944, 1056
Tru9I TTAA 2 cut(s) 944, 1056
TscAI CASTG 3 cut(s) 27, 312, 720
TseFI GTSAC 1 cut(s) 255
TseI GCWGC 2 cut(s) 182, 316
Tsp45I GTSAC 1 cut(s) 255
TspDTI ATGAA 6 cut(s) 108, 561, 974, 1079, 1149, 1328
TspGWI ACGGA 1 cut(s) 530
TspRI CASTG 3 cut(s) 27, 312, 720
Van91I CCANNNNNTGG 1 cut(s) 217
VneI GTGCAC 1 cut(s) 1254
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 3 cut(s) 236, 402, 1305
XhoI CTCGAG 1 cut(s) 1287
XmnI GAANNNNTTC 3 cut(s) 380, 756, 969
XspI CTAG 4 cut(s) 320, 500, 725, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.