RLG00000006978

65-kDa microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0007124
Physical Location & Seq
Forward (+)
12325547 .. 12329673
4127 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006978

Sequence Viewer

Length: 1779 bp
ATGGGTTCATTTCAAGCACCGGTAGGGAGAAGCTCTGCGCTTCCTGAAACATCATGTGGATATTTGCTTCAAGAACTACAGATGATTTGGAATGAAATTGGACAAGATCAGTTGGAAAGGGAGAAGGTACTGGTGGATTTAGAGCAGGAATGTCTGGAGGTCTATAGGAGGAAGGTTGATGCTGCTAATACCTCAAGAGCTCGCCTGCACCAGGAGTTGGCAGAAGCTGAGGCTGAATTTACTCATCTTCTTTTGTCACTTGGCGAACGGTCTCTCCCCGGACGGCCAGAAAAAATGGTGGGAACACTGAAAGAGCAGCTAGATTCCATCACACCAGCTCTGCGAGACATGAGATTGAGGAAAGAAGATAGGGTGAACAAGTTCCGAGCTGTACAAGGGCAAATTCAGAAGATCTCTGCAGAAATAGCAGGACAGTCAGAGTATGAGAACTCATCATCAGATGTCAGGGTAAATGAGAATGATCTTTCATTGAAGAAACTAGAGGAGTACCAGACAGAGCTACAAAGACTCCACAATGACAGGAATGAAAGGCTCAAGCGGGTGGAGAAATATATAAACTCCATCCAAAAGTTGTCTTCAATATTAGGAACAGACTCCTCTATGGTCATCACCAAGGTTCACCCAAGCTTGAATGAATTATGTGGGATAGCCAAGAACATAAGCAACAGTATTTTGGCTAAACTCAACAGCACAGTGGAATCCCTAGAGGAAGAAAAGAAAAAGCGGCTTGAAAAGCTTCACCATCTTGGTAAAGCATTGACAAACTTATGGAATTTCATGGACACGCCCTATAAAGATCGTCAATTGTTTTCCCATGTTACCTGCTTATTATCAGTCTCATCAGCAGAAGTATCAGATCCAGGAAGCCTAACCCAAAAAAAAATCCAGCAGGCTGAGGCTGAAGTCAAGAGACTGGATCAATTGAAAGTAAGCAAGATGAAAGAACTATTTCTCAAGAAACAGAATGAGCTCAAGGAGATATGCAAAAAATCACACATGGAGATTCCTGTGCAAGCAAAGATGGATAATATAATTAACCTCATGAAATCTGGGGAAATTGACCATGCAGATCTTGTTATGAGCATGGATCAACAGATATCAAGAGCAGAAGATGAGGCTTGTAGCAGAAAGACAATTATGGAAAAGGTGGAAAAGTGGATGCTTGCACGTGATGAGGAGCGCTGGTTGGAAGAATATGGAAGAGACGAAAACCGATACTCAGTCAGAAGAGGTGCACACAAGAACCTGAGACGCGCAGAACGTGCTCGAGTATTAGTCAGCAAAATTCCAGCTTTGGTGGATTTGCTGATAGAAAACACTAAGAGTTGGGAAGAAGAAAGAAAGAAGACTTTCTTATATGATGAGGTACCTCTCCTAGCAATGTTGGAAGAGTATATTATGCTGAGGCAGGAAAAGGAGGAGGAGAGGCAAAGACAAAGGGAAGTGAAGTTCCAAGGCCTAATGGGGCAACATCAGCCAGAAAATTTGTTCCTGCCCAGTCCAAGCAGCAGTCCTAGGCGAACTTCAAACAGAAGTGTAAATGGAGGTTTTAGCACTGCTATGCCTCTGAACAGAAGGCTTTCTCTGGGCCTTCAGAAACTGGGACCAAACAGCATAGATTTAGGCACTCAAGGCATATCAAAGGAAGGTAAGAAGGCACAGAGACAAAAGATGTTTGCAAGACCTGGTCTTGTTTCTCCTGCTAGAGATGAAACAGCTTCAGTGGTATCAACATTTTCTGGCCCACTATCTCCCTGA

Protein Analysis

593

Amino Acids

67.79

Weight (kDa)

8.72

Isoelectric Point (pI)

54.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 22 - 492 1.1e-71 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 851
Acc65I GGTACC 1 cut(s) 1387
AccB1I GGYRCC 1 cut(s) 1387
AccB7I CCANNNNNTGG 1 cut(s) 217
AccII CGCG 1 cut(s) 1275
AciI CCGC 2 cut(s) 559, 745
AclWI GGATC 3 cut(s) 872, 945, 1116
AcoI YGGCCR 1 cut(s) 284
AcsI RAATTY 5 cut(s) 236, 402, 793, 1305, 1504
AcuI CTGAAG 3 cut(s) 942, 1598, 1725
AcvI CACGTG 1 cut(s) 1190
AfaI GTAC 4 cut(s) 129, 393, 509, 1389
AfeI AGCGCT 1 cut(s) 1202
AfiI CCNNNNNNNGG 2 cut(s) 211, 217
AgeI ACCGGT 1 cut(s) 19
AgsI TTSAA 8 cut(s) 14, 71, 493, 600, 652, 752, 946, 1548
AjnI CCWGG 3 cut(s) 210, 880, 1705
AloI GAACNNNNNNTCC 4 cut(s) 258, 290, 1454, 1486
Alw21I GWGCWC 4 cut(s) 202, 993, 1258, 1288
Alw26I GTCTC 7 cut(s) 276, 339, 862, 925, 1218, 1264, 1678
Alw44I GTGCAC 1 cut(s) 1254
AlwI GGATC 3 cut(s) 872, 945, 1116
AlwNI CAGNNNCTG 2 cut(s) 227, 1621
Ama87I CYCGRG 1 cut(s) 1287
Aor51HI AGCGCT 1 cut(s) 1202
AoxI GGCC 4 cut(s) 284, 1477, 1609, 1762
ApaLI GTGCAC 1 cut(s) 1254
ApeKI GCWGC 3 cut(s) 182, 316, 1527
ApoI RAATTY 5 cut(s) 236, 402, 793, 1305, 1504
AsiGI ACCGGT 1 cut(s) 19
Asp700I GAANNNNTTC 5 cut(s) 380, 756, 969, 1370, 1600
Asp718I GGTACC 1 cut(s) 1387
AspA2I CCTAGG 1 cut(s) 1535
AspLEI GCGC 3 cut(s) 40, 1203, 1277
AspS9I GGNCC 3 cut(s) 1609, 1625, 1763
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 4 cut(s) 385, 622, 632, 752
AvaI CYCGRG 1 cut(s) 1287
AvaII GGWCC 1 cut(s) 1625
AvrII CCTAGG 1 cut(s) 1535
BaeGI GKGCMC 1 cut(s) 1258
BanI GGYRCC 1 cut(s) 1387
BanII GRGCYC 2 cut(s) 202, 993
BbrPI CACGTG 1 cut(s) 1190
BbsI GAAGAC 2 cut(s) 588, 1373
Bbv12I GWGCWC 4 cut(s) 202, 993, 1258, 1288
BbvCI CCTCAGC 3 cut(s) 228, 915, 1424
BbvI GCAGC 3 cut(s) 169, 328, 1539
BccI CCATC 4 cut(s) 335, 590, 771, 1036
BceAI ACGGC 1 cut(s) 299
BciT130I CCWGG 3 cut(s) 212, 882, 1707
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 7 cut(s) 276, 339, 862, 925, 1218, 1264, 1678
BfaI CTAG 6 cut(s) 320, 500, 725, 1397, 1536, 1725
BfmI CTRYAG 3 cut(s) 77, 163, 417
BfoI RGCGCY 1 cut(s) 1204
BfuAI ACCTGC 1 cut(s) 851
BglII AGATCT 2 cut(s) 411, 1090
BisI GCNGC 4 cut(s) 183, 317, 746, 1528
BlnI CCTAGG 1 cut(s) 1535
BlsI GCNGC 4 cut(s) 184, 318, 747, 1529
Bme1390I CCNGG 4 cut(s) 212, 279, 882, 1707
Bme18I GGWCC 1 cut(s) 1625
BmeT110I CYCGRG 1 cut(s) 1287
BmgT120I GGNCC 3 cut(s) 1609, 1625, 1763
BmiI GGNNCC 2 cut(s) 1389, 1626
BmrFI CCNGG 4 cut(s) 212, 279, 882, 1707
BmrI ACTGGG 2 cut(s) 1512, 1631
BmsI GCATC 2 cut(s) 169, 1170
BmuI ACTGGG 2 cut(s) 1512, 1631
BpiI GAAGAC 2 cut(s) 588, 1373
BpmI CTGGAG 1 cut(s) 176
Bpu10I CCTNAGC 3 cut(s) 228, 915, 1424
BpuEI CTTGAG 5 cut(s) 178, 539, 959, 977, 1635
BpuMI CCSGG 1 cut(s) 279
BsaAI YACGTR 1 cut(s) 1190
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 4 cut(s) 277, 633, 1474, 1535
BsaWI WCCGGW 1 cut(s) 19
BsaXI ACNNNNNCTCC 6 cut(s) 258, 288, 513, 543, 1190, 1220
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 217
Bse118I RCCGGY 1 cut(s) 19
Bse1I ACTGG 4 cut(s) 135, 939, 1518, 1626
Bse3DI GCAATG 1 cut(s) 1407
BseBI CCWGG 3 cut(s) 212, 882, 1707
BseDI CCNNGG 4 cut(s) 277, 633, 1474, 1535
BseGI GGATG 2 cut(s) 582, 1185
BseLI CCNNNNNNNGG 2 cut(s) 211, 217
BseMI GCAATG 1 cut(s) 1407
BseMII CTCAG 5 cut(s) 219, 906, 1254, 1259, 1415
BseNI ACTGG 4 cut(s) 135, 939, 1518, 1626
BseRI GAGGAG 5 cut(s) 518, 607, 1211, 1454, 1457
BseSI GKGCMC 1 cut(s) 1258
BseXI GCAGC 3 cut(s) 169, 328, 1539
BsgI GTGCAG 1 cut(s) 191
Bsh1236I CGCG 1 cut(s) 1275
BshFI GGCC 4 cut(s) 286, 1479, 1611, 1764
BshNI GGYRCC 1 cut(s) 1387
BshTI ACCGGT 1 cut(s) 19
BsiHKAI GWGCWC 4 cut(s) 202, 993, 1258, 1288
BsiHKCI CYCGRG 1 cut(s) 1287
BsiSI CCGG 2 cut(s) 20, 279
BslFI GGGAC 1 cut(s) 1638
BslI CCNNNNNNNGG 2 cut(s) 211, 217
BsmAI GTCTC 7 cut(s) 276, 339, 862, 925, 1218, 1264, 1678
BsmBI CGTCTC 2 cut(s) 1218, 1264
BsmFI GGGAC 1 cut(s) 1638
BsnI GGCC 4 cut(s) 286, 1479, 1611, 1764
Bso31I GGTCTC 1 cut(s) 276
BsoBI CYCGRG 1 cut(s) 1287
Bsp1286I GDGCHC 4 cut(s) 202, 993, 1258, 1288
Bsp1407I TGTACA 1 cut(s) 391
Bsp143I GATC 8 cut(s) 106, 411, 481, 817, 877, 937, 1090, 1108
BspACI CCGC 2 cut(s) 559, 745
BspANI GGCC 4 cut(s) 286, 1479, 1611, 1764
BspCNI CTCAG 5 cut(s) 220, 907, 1253, 1260, 1416
BspFNI CGCG 1 cut(s) 1275
BspHI TCATGA 1 cut(s) 1062
BspLI GGNNCC 2 cut(s) 1389, 1626
BspMAI CTGCAG 1 cut(s) 421
BspMI ACCTGC 1 cut(s) 851
BspPI GGATC 3 cut(s) 872, 945, 1116
BspT107I GGYRCC 1 cut(s) 1387
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 1407
BsrFI RCCGGY 1 cut(s) 19
BsrGI TGTACA 1 cut(s) 391
BsrI ACTGG 4 cut(s) 135, 939, 1518, 1626
BssAI RCCGGY 1 cut(s) 19
BssECI CCNNGG 4 cut(s) 277, 633, 1474, 1535
BssMI GATC 8 cut(s) 106, 411, 481, 817, 877, 937, 1090, 1108
BssT1I CCWWGG 3 cut(s) 633, 1474, 1535
Bst2UI CCWGG 3 cut(s) 212, 882, 1707
Bst4CI ACNGT 4 cut(s) 270, 435, 689, 715
Bst6I CTCTTC 3 cut(s) 1216, 1243, 1404
BstAPI GCANNNNNTGC 1 cut(s) 1283
BstAUI TGTACA 1 cut(s) 391
BstBAI YACGTR 1 cut(s) 1190
BstC8I GCNNGC 5 cut(s) 202, 206, 912, 1035, 1185
BstDEI CTNAG 6 cut(s) 228, 915, 1240, 1268, 1341, 1424
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 2 cut(s) 582, 1185
BstFNI CGCG 1 cut(s) 1275
BstH2I RGCGCY 1 cut(s) 1204
BstHHI GCGC 3 cut(s) 40, 1203, 1277
BstKTI GATC 8 cut(s) 109, 414, 484, 820, 880, 940, 1093, 1111
BstMAI GTCTC 7 cut(s) 276, 339, 862, 925, 1218, 1264, 1678
BstMBI GATC 8 cut(s) 106, 411, 481, 817, 877, 937, 1090, 1108
BstMWI GCNNNNNNNGC 5 cut(s) 425, 754, 1283, 1495, 1653
BstNI CCWGG 3 cut(s) 212, 882, 1707
BstSCI CCNGG 4 cut(s) 210, 277, 880, 1705
BstSFI CTRYAG 3 cut(s) 77, 163, 417
BstSLI GKGCMC 1 cut(s) 1258
BstUI CGCG 1 cut(s) 1275
BstV1I GCAGC 3 cut(s) 169, 328, 1539
BstV2I GAAGAC 2 cut(s) 588, 1373
BstX2I RGATCY 3 cut(s) 411, 877, 1090
BstYI RGATCY 3 cut(s) 411, 877, 1090
BsuRI GGCC 4 cut(s) 286, 1479, 1611, 1764
BtsCI GGATG 2 cut(s) 582, 1185
BtsI GCAGTG 1 cut(s) 1575
BtsIMutI CAGTG 4 cut(s) 305, 720, 1575, 1749
BveI ACCTGC 1 cut(s) 851
Cac8I GCNNGC 5 cut(s) 202, 206, 912, 1035, 1185
CaiI CAGNNNCTG 2 cut(s) 227, 1621
CciI TCATGA 1 cut(s) 1062
CfoI GCGC 3 cut(s) 40, 1203, 1277
Cfr10I RCCGGY 1 cut(s) 19
Cfr13I GGNCC 3 cut(s) 1609, 1625, 1763
CseI GACGC 1 cut(s) 1281
CsiI ACCWGGT 1 cut(s) 1705
Csp6I GTAC 4 cut(s) 128, 392, 508, 1388
CspAI ACCGGT 1 cut(s) 19
CviAII CATG 8 cut(s) 54, 349, 799, 836, 1018, 1063, 1085, 1105
CviQI GTAC 4 cut(s) 128, 392, 508, 1388
DdeI CTNAG 6 cut(s) 228, 915, 1240, 1268, 1341, 1424
DpnI GATC 8 cut(s) 108, 413, 483, 819, 879, 939, 1092, 1110
DpnII GATC 8 cut(s) 106, 411, 481, 817, 877, 937, 1090, 1108
EaeI YGGCCR 1 cut(s) 284
Eam1104I CTCTTC 3 cut(s) 1216, 1243, 1404
EarI CTCTTC 3 cut(s) 1216, 1243, 1404
Ecl136II GAGCTC 2 cut(s) 200, 991
Eco130I CCWWGG 3 cut(s) 633, 1474, 1535
Eco147I AGGCCT 1 cut(s) 1479
Eco24I GRGCYC 2 cut(s) 202, 993
Eco31I GGTCTC 1 cut(s) 276
Eco32I GATATC 1 cut(s) 1119
Eco47I GGWCC 1 cut(s) 1625
Eco47III AGCGCT 1 cut(s) 1202
Eco53kI GAGCTC 2 cut(s) 200, 991
Eco57I CTGAAG 3 cut(s) 942, 1598, 1725
Eco72I CACGTG 1 cut(s) 1190
Eco88I CYCGRG 1 cut(s) 1287
EcoICRI GAGCTC 2 cut(s) 200, 991
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoRII CCWGG 3 cut(s) 210, 880, 1705
EcoRV GATATC 1 cut(s) 1119
EcoT14I CCWWGG 3 cut(s) 633, 1474, 1535
EcoT38I GRGCYC 2 cut(s) 202, 993
ErhI CCWWGG 3 cut(s) 633, 1474, 1535
Esp3I CGTCTC 2 cut(s) 1218, 1264
FaeI CATG 8 cut(s) 57, 352, 802, 839, 1021, 1066, 1088, 1108
FalI AAGNNNNNCTT 2 cut(s) 1358, 1390
FaqI GGGAC 1 cut(s) 1638
FatI CATG 8 cut(s) 53, 348, 798, 835, 1017, 1062, 1084, 1104
FauI CCCGC 1 cut(s) 552
Fnu4HI GCNGC 4 cut(s) 183, 317, 746, 1528
FokI GGATG 2 cut(s) 569, 1192
FriOI GRGCYC 2 cut(s) 202, 993
Fsp4HI GCNGC 4 cut(s) 183, 317, 746, 1528
FspBI CTAG 6 cut(s) 320, 500, 725, 1397, 1536, 1725
GlaI GCGC 3 cut(s) 39, 1202, 1276
GluI GCNGC 4 cut(s) 183, 317, 746, 1528
GsuI CTGGAG 1 cut(s) 176
HaeII RGCGCY 1 cut(s) 1204
HaeIII GGCC 4 cut(s) 286, 1479, 1611, 1764
HapII CCGG 2 cut(s) 20, 279
HgaI GACGC 1 cut(s) 1281
HhaI GCGC 3 cut(s) 40, 1203, 1277
Hin1II CATG 8 cut(s) 57, 352, 802, 839, 1021, 1066, 1088, 1108
Hin6I GCGC 3 cut(s) 38, 1201, 1275
HinP1I GCGC 3 cut(s) 38, 1201, 1275
HindIII AAGCTT 2 cut(s) 646, 755
HinfI GANTC 5 cut(s) 323, 528, 614, 719, 1024
HpaII CCGG 2 cut(s) 20, 279
HphI GGTGA 4 cut(s) 385, 622, 632, 752
Hpy166II GTNNAC 3 cut(s) 376, 640, 1256
Hpy188I TCNGA 8 cut(s) 386, 408, 439, 460, 877, 1247, 1590, 1617
Hpy188III TCNNGA 8 cut(s) 44, 71, 155, 195, 928, 976, 1063, 1122
Hpy8I GTNNAC 3 cut(s) 376, 640, 1256
HpyAV CCTTC 6 cut(s) 118, 166, 1590, 1622, 1661, 1669
HpyCH4III ACNGT 4 cut(s) 270, 435, 689, 715
HpyCH4IV ACGT 2 cut(s) 1189, 1282
HpyCH4V TGCA 8 cut(s) 208, 419, 1005, 1033, 1088, 1187, 1256, 1700
HpyF10VI GCNNNNNNNGC 5 cut(s) 425, 754, 1283, 1495, 1653
HpyF3I CTNAG 6 cut(s) 228, 915, 1240, 1268, 1341, 1424
HpySE526I ACGT 2 cut(s) 1189, 1282
Hsp92II CATG 8 cut(s) 57, 352, 802, 839, 1021, 1066, 1088, 1108
HspAI GCGC 3 cut(s) 38, 1201, 1275
KpnI GGTACC 1 cut(s) 1391
Kzo9I GATC 8 cut(s) 106, 411, 481, 817, 877, 937, 1090, 1108
LmnI GCTCC 1 cut(s) 1198
Lsp1109I GCAGC 3 cut(s) 169, 328, 1539
LweI GCATC 2 cut(s) 169, 1170
MabI ACCWGGT 1 cut(s) 1705
MaeI CTAG 6 cut(s) 320, 500, 725, 1397, 1536, 1725
MaeII ACGT 2 cut(s) 1189, 1282
MaeIII GTNAC 2 cut(s) 255, 838
MalI GATC 8 cut(s) 108, 413, 483, 819, 879, 939, 1092, 1110
MboI GATC 8 cut(s) 106, 411, 481, 817, 877, 937, 1090, 1108
MfeI CAATTG 2 cut(s) 824, 941
MflI RGATCY 3 cut(s) 411, 877, 1090
MhlI GDGCHC 4 cut(s) 202, 993, 1258, 1288
MlyI GAGTC 2 cut(s) 522, 608
MmeI TCCRAC 3 cut(s) 93, 1188, 1386
MroXI GAANNNNTTC 5 cut(s) 380, 756, 969, 1370, 1600
MseI TTAA 1 cut(s) 1056
MslI CAYNNNNRTG 1 cut(s) 1580
MspI CCGG 2 cut(s) 20, 279
MspR9I CCNGG 4 cut(s) 212, 279, 882, 1707
MunI CAATTG 2 cut(s) 824, 941
MvaI CCWGG 3 cut(s) 212, 882, 1707
MvnI CGCG 1 cut(s) 1275
MwoI GCNNNNNNNGC 5 cut(s) 425, 754, 1283, 1495, 1653
NciI CCSGG 1 cut(s) 279
NdeII GATC 8 cut(s) 106, 411, 481, 817, 877, 937, 1090, 1108
NlaIII CATG 8 cut(s) 57, 352, 802, 839, 1021, 1066, 1088, 1108
NlaIV GGNNCC 2 cut(s) 1389, 1626
NmuCI GTSAC 1 cut(s) 255
PaeR7I CTCGAG 1 cut(s) 1287
PagI TCATGA 1 cut(s) 1062
PceI AGGCCT 1 cut(s) 1479
PcsI WCGNNNNNNNCGW 1 cut(s) 1279
PdmI GAANNNNTTC 5 cut(s) 380, 756, 969, 1370, 1600
PfeI GAWTC 3 cut(s) 323, 719, 1024
PflFI GACNNNGTC 1 cut(s) 1707
PflMI CCANNNNNTGG 1 cut(s) 217
PfoI TCCNGGA 1 cut(s) 880
PinAI ACCGGT 1 cut(s) 19
PkrI GCNGC 4 cut(s) 184, 318, 747, 1529
PleI GAGTC 2 cut(s) 522, 608
PmaCI CACGTG 1 cut(s) 1190
PmlI CACGTG 1 cut(s) 1190
PpsI GAGTC 2 cut(s) 522, 608
Ppu21I YACGTR 1 cut(s) 1190
Psp124BI GAGCTC 2 cut(s) 202, 993
Psp6I CCWGG 3 cut(s) 210, 880, 1705
PspCI CACGTG 1 cut(s) 1190
PspGI CCWGG 3 cut(s) 210, 880, 1705
PspN4I GGNNCC 2 cut(s) 1389, 1626
PspPI GGNCC 3 cut(s) 1609, 1625, 1763
PspXI VCTCGAGB 1 cut(s) 1287
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 2 cut(s) 227, 1621
PsuI RGATCY 3 cut(s) 411, 877, 1090
PsyI GACNNNGTC 1 cut(s) 1707
RsaI GTAC 4 cut(s) 129, 393, 509, 1389
RsaNI GTAC 4 cut(s) 128, 392, 508, 1388
RseI CAYNNNNRTG 1 cut(s) 1580
SacI GAGCTC 2 cut(s) 202, 993
SaqAI TTAA 1 cut(s) 1056
SatI GCNGC 4 cut(s) 183, 317, 746, 1528
Sau3AI GATC 8 cut(s) 106, 411, 481, 817, 877, 937, 1090, 1108
Sau96I GGNCC 3 cut(s) 1609, 1625, 1763
SchI GAGTC 2 cut(s) 522, 608
ScrFI CCNGG 4 cut(s) 212, 279, 882, 1707
SduI GDGCHC 4 cut(s) 202, 993, 1258, 1288
SexAI ACCWGGT 1 cut(s) 1705
SfaNI GCATC 2 cut(s) 169, 1170
SfcI CTRYAG 3 cut(s) 77, 163, 417
Sfr274I CTCGAG 1 cut(s) 1287
SinI GGWCC 1 cut(s) 1625
SlaI CTCGAG 1 cut(s) 1287
SmiMI CAYNNNNRTG 1 cut(s) 1580
SmlI CTYRAG 6 cut(s) 193, 554, 974, 992, 1287, 1650
SmoI CTYRAG 6 cut(s) 193, 554, 974, 992, 1287, 1650
SseBI AGGCCT 1 cut(s) 1479
SsiI CCGC 2 cut(s) 559, 745
SspI AATATT 1 cut(s) 603
SspMI CTAG 6 cut(s) 320, 500, 725, 1397, 1536, 1725
SstI GAGCTC 2 cut(s) 202, 993
StuI AGGCCT 1 cut(s) 1479
StyD4I CCNGG 4 cut(s) 210, 277, 880, 1705
StyI CCWWGG 3 cut(s) 633, 1474, 1535
TaaI ACNGT 4 cut(s) 270, 435, 689, 715
TaiI ACGT 2 cut(s) 1192, 1285
TaqI TCGA 1 cut(s) 1288
TatI WGTACW 1 cut(s) 391
TauI GCSGC 1 cut(s) 748
TfiI GAWTC 3 cut(s) 323, 719, 1024
Tru1I TTAA 1 cut(s) 1056
Tru9I TTAA 1 cut(s) 1056
TscAI CASTG 4 cut(s) 312, 720, 1582, 1749
TseFI GTSAC 1 cut(s) 255
TseI GCWGC 3 cut(s) 182, 316, 1527
Tsp45I GTSAC 1 cut(s) 255
TspDTI ATGAA 8 cut(s) 108, 477, 561, 669, 787, 974, 1079, 1746
TspRI CASTG 4 cut(s) 312, 720, 1582, 1749
Tth111I GACNNNGTC 1 cut(s) 1707
Van91I CCANNNNNTGG 1 cut(s) 217
VneI GTGCAC 1 cut(s) 1254
VpaK11BI GGWCC 1 cut(s) 1625
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 5 cut(s) 236, 402, 793, 1305, 1504
XhoI CTCGAG 1 cut(s) 1287
XmaJI CCTAGG 1 cut(s) 1535
XmnI GAANNNNTTC 5 cut(s) 380, 756, 969, 1370, 1600
XspI CTAG 6 cut(s) 320, 500, 725, 1397, 1536, 1725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.