Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007124

Physical Location & Seq

Genomic Coordinates Forward (+)

8409832 .. 8414048

Gene Structure 4217 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1118300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1788 bp

ATGGGTTCGTTTCAGACGCCGATGGGAATGCGAAGCTCCACGCTTTTGGAAACTTCGTGCGGATACTTGCTTCAGGAATTGCAGATGATTTGGAATGAAGTTGGGCAAGATCAGTTTGAAAGGGAGAAGGTGCTGCTGGATTTAGAGCAAGAATGTCTGGAGGTTTATAGGAAGAAGGTTGATACTGCAAATACCTCGAGAGCTCGCTTGCACCAGGAGTTGGCTGAAGCTGAGGCTGAGTTCACCCATCTTCTTCTGTCGCTGGGCGAACGGTCTCTCCCTGGACGGCCAGAAAAATTGGCGGGAACGCTGAAAGAGCAGCTGGATTCACTCACGCCGGCATTGAGGGAGATGAGATTGAGGAAAAAAGATAGGGTGAATAAGTTTCGAGCAGTACAAGGGCAAATTCAGAAAATTTCTGCAGAAATAGCAGGTCAGTCAGAGTACGGAGACTTATCATCAAATATCGTGGTGAATGAGAATGATCTTTCGTTGAAAAAACTAGAGGAGTATCAGACGGAGCTACAAACACTCCACAACGAGAAGAATGAAAGGCTCATGAGAGTCGAAAAATATATAGATGCAGTCCACAATTTGTCTTCGATATTAGGAACAGAAGCCTCAATGGTAATCACCAAGGTTCATCCAAGCTTGAATGAATTATGTGGGTTGGCCAAAAACATAAGCAACAATATTTTGGCTAAACTTAACAGCACAGTTGAATCCCTAGAGGAAGAAAAGCAAAAGCGACTCGAAAAGCTTCACCATCTTGGCAAAGTATTGACAAACTTATGGGATCTCATGGACACACCCTATAAAGATCGTCAATCGTTTTCCCATGTCACTGGCTTATTATCACTCTCATCGGCAGAAGTATCAGATCCAGGAAGCCTGACCCTAAATATAATACAACAGGCCGAAGCTGAAGTCAAGAGACTGGATCACTTAAAAGCAAGCAAGATGAAAGAACTATTTTTCAAGAAACAGAATGAGCTCAAGGAGATATGCAATAAATCGCACATGGAAATTCCTTTGCAATCAGAGATTGATAATCTTATTAACCTCATCAACTCTGGGGAGATTGACCATGCAGATCTCCTAATGAGCTTGGATCAACAGATATCAAGAGCAAAGGAAGAAGCTTCTAGCAGAATGACTATCATGGAAAAGGTGGAGAAGTGGATGCTAGCACGTGACGAGGAGCGTTGGTTGGAAGAATACACTAGAGACGAGAACCGGTACTCAGTCAGCAGAGGTGCACACAAGAACCTGAGACGTGCAGAACGTGCCCGAGTATTAGTCAACAAAATCCCAGCTTTGGTGGATTTGCTAATAACAAATACTAAGAGTTGGGAAGAAGAAAGAAAGAAAACTTTCTTGTATGATGAGGTACCACTCCTAGAAATGTTGGAAGAGTATAATGTGCTAAGGCAGGAAAAGGAGGAGGATAAGCAAAGACAACGGGAAGTGAAGAAGGTCCAAAGCCAAGTAGTAGTTGAGCAAGAAAATCTGTTCATAACCAGGCCAAGCACCAGTACAAGGCGAACTTCAAGCAGGACTTTGAATGGAGGTTTTAGCAACGCCATGCCTCTAAACAGAAGGCTTTCACTAGGCCTTCAGCAATTGGGACCAAACAGTGTAAACTCAGCCCCTCAAGGCATTTCCTTTATGAAGGAAGGCAAGAAGGCACACAGACAAAAGATGTCTGCTCGACCTGGCCTTGTTTCTCATCTTAGAGATGAAACAGCTTCAGTGGTGTCAACGTTTTCTGGTCCACAATCTCCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0009524 GO:0015630 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0099080 GO:0099081 GO:0099512 GO:0099513

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MAP65_ASE1

Protein Analysis

596

Amino Acids

68.1

Weight (kDa)

6.59

Isoelectric Point (pI)

52.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MAP65_ASE1	PF03999	23 - 517	2.7e-73	Microtubule associated protein (MAP65/ASE1 family)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007124)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27920 AT1G27920
fragaria_vesca	FvH4_4g23850 FvH4_4g23850
malus_domestica	MD13G1118200.v1.1 MD16G1118300.v1.1
prunus_persica	Prupe.1G226800_v2.0.a1 Prupe.1G226800_v2.0.a1 Prupe.1G226800_v2.0.a1 Prupe.1G226800_v2.0.a1
pyrus_communis	pycom13g10290 pycom16g10130
rosa_chinensis	RchiOBHm_Chr4g0430421
rosa_laevigata	RLG00000006978
rosa_multiflora	Rmu_sc0000131.1_g000043 Rmu_sc0016735.1_g000001
rosa_roxburghii	Rroxscaffold_5G00372200
rosa_rugosa	Rorug04G0242800 Rorug04G0242900 Rorug04G0243000
rosa_samantha	Rh4AG299300 Rh4BG306100 Rh4CG321900 Rh4DG303500
rosa_wichuraiana	Rw4G025990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	422
Acc65I	GGTACC	1 cut(s)	1390
AccB1I	GGYRCC	1 cut(s)	1390
AccB7I	CCANNNNNTGG	1 cut(s)	220
AciI	CCGC	2 cut(s)	60, 302
AclI	AACGTT	1 cut(s)	1763
AclWI	GGATC	4 cut(s)	804, 875, 948, 1119
AcoI	YGGCCR	2 cut(s)	287, 672
AcsI	RAATTY	3 cut(s)	405, 414, 1026
AcuI	CTGAAG	5 cut(s)	56, 246, 945, 1601, 1734
AcvI	CACGTG	1 cut(s)	1193
AcyI	GRCGYC	1 cut(s)	17
AfaI	GTAC	5 cut(s)	396, 446, 1241, 1392, 1537
AfiI	CCNNNNNNNGG	3 cut(s)	220, 1318, 1539
AgeI	ACCGGT	1 cut(s)	1236
AgsI	TTSAA	7 cut(s)	119, 496, 655, 722, 979, 1551, 1564
AjiI	CACGTC	1 cut(s)	1277
AjnI	CCWGG	5 cut(s)	213, 280, 883, 1520, 1714
AloI	GAACNNNNNNTCC	2 cut(s)	261, 293
Alw21I	GWGCWC	3 cut(s)	205, 996, 1261
Alw26I	GTCTC	5 cut(s)	279, 444, 928, 1221, 1267
Alw44I	GTGCAC	1 cut(s)	1257
AlwI	GGATC	4 cut(s)	804, 875, 948, 1119
Ama87I	CYCGRG	2 cut(s)	196, 1290
AoxI	GGCC	6 cut(s)	287, 672, 915, 1523, 1612, 1717
ApaLI	GTGCAC	1 cut(s)	1257
ApeKI	GCWGC	2 cut(s)	133, 319
ApoI	RAATTY	3 cut(s)	405, 414, 1026
ArsI	GACNNNNNNTTYG	2 cut(s)	912, 944
AsiGI	ACCGGT	1 cut(s)	1236
Asp700I	GAANNNNTTC	3 cut(s)	759, 1373, 1603
Asp718I	GGTACC	1 cut(s)	1390
AspS9I	GGNCC	3 cut(s)	1477, 1628, 1772
AsuHPI	GGTGA	5 cut(s)	235, 388, 484, 625, 755
AsuNHI	GCTAGC	1 cut(s)	1186
AvaI	CYCGRG	2 cut(s)	196, 1290
AvaII	GGWCC	3 cut(s)	1477, 1628, 1772
BaeGI	GKGCMC	2 cut(s)	1261, 1291
BalI	TGGCCA	1 cut(s)	674
BanI	GGYRCC	1 cut(s)	1390
BanII	GRGCYC	2 cut(s)	205, 996
BbrPI	CACGTG	1 cut(s)	1193
BbsI	GAAGAC	1 cut(s)	591
Bbv12I	GWGCWC	3 cut(s)	205, 996, 1261
BbvCI	CCTCAGC	1 cut(s)	231
BbvI	GCAGC	2 cut(s)	120, 331
BccI	CCATC	3 cut(s)	16, 255, 774
BceAI	ACGGC	1 cut(s)	302
BcgI	CGANNNNNNTGC	4 cut(s)	10, 44, 177, 211
BciT130I	CCWGG	5 cut(s)	215, 282, 885, 1522, 1716
BciVI	GTATCC	1 cut(s)	56
BcoDI	GTCTC	5 cut(s)	279, 444, 928, 1221, 1267
BfaI	CTAG	7 cut(s)	503, 728, 1146, 1187, 1224, 1400, 1610
BfmI	CTRYAG	1 cut(s)	420
BfuAI	ACCTGC	1 cut(s)	422
BfuI	GTATCC	1 cut(s)	56
BglII	AGATCT	1 cut(s)	1093
BisI	GCNGC	2 cut(s)	134, 320
BlsI	GCNGC	2 cut(s)	135, 321
Bme1390I	CCNGG	5 cut(s)	215, 282, 885, 1522, 1716
Bme18I	GGWCC	3 cut(s)	1477, 1628, 1772
BmeT110I	CYCGRG	2 cut(s)	196, 1290
BmgBI	CACGTC	1 cut(s)	1277
BmgT120I	GGNCC	3 cut(s)	1477, 1628, 1772
BmiI	GGNNCC	2 cut(s)	1392, 1629
BmrFI	CCNGG	5 cut(s)	215, 282, 885, 1522, 1716
BmsI	GCATC	2 cut(s)	571, 1173
BmtI	GCTAGC	1 cut(s)	1190
BpiI	GAAGAC	1 cut(s)	591
BpmI	CTGGAG	1 cut(s)	179
Bpu10I	CCTNAGC	2 cut(s)	231, 1427
BpuEI	CTTGAG	2 cut(s)	980, 1638
BsaAI	YACGTR	1 cut(s)	1193
BsaHI	GRCGYC	1 cut(s)	17
BsaI	GGTCTC	1 cut(s)	279
BsaJI	CCNNGG	2 cut(s)	280, 636
BsaWI	WCCGGW	1 cut(s)	1236
BsaXI	ACNNNNNCTCC	6 cut(s)	261, 291, 516, 546, 1193, 1223
Bsc4I	CCNNNNNNNGG	3 cut(s)	220, 1318, 1539
Bse118I	RCCGGY	2 cut(s)	337, 1236
Bse1I	ACTGG	3 cut(s)	850, 942, 1533
BseBI	CCWGG	5 cut(s)	215, 282, 885, 1522, 1716
BseDI	CCNNGG	2 cut(s)	280, 636
BseGI	GGATG	2 cut(s)	643, 1188
BseLI	CCNNNNNNNGG	3 cut(s)	220, 1318, 1539
BseMII	CTCAG	5 cut(s)	222, 228, 1257, 1262, 1659
BseNI	ACTGG	3 cut(s)	850, 942, 1533
BseRI	GAGGAG	3 cut(s)	521, 1214, 1457
BseSI	GKGCMC	2 cut(s)	1261, 1291
BseXI	GCAGC	2 cut(s)	120, 331
BseYI	CCCAGC	2 cut(s)	262, 1312
BsgI	GTGCAG	1 cut(s)	1299
BshFI	GGCC	6 cut(s)	289, 674, 917, 1525, 1614, 1719
BshNI	GGYRCC	1 cut(s)	1390
BshTI	ACCGGT	1 cut(s)	1236
BsiHKAI	GWGCWC	3 cut(s)	205, 996, 1261
BsiHKCI	CYCGRG	2 cut(s)	196, 1290
BsiSI	CCGG	2 cut(s)	338, 1237
BslFI	GGGAC	1 cut(s)	1641
BslI	CCNNNNNNNGG	3 cut(s)	220, 1318, 1539
BsmAI	GTCTC	5 cut(s)	279, 444, 928, 1221, 1267
BsmBI	CGTCTC	2 cut(s)	1221, 1267
BsmFI	GGGAC	1 cut(s)	1641
BsmI	GAATGC	1 cut(s)	33
BsnI	GGCC	6 cut(s)	289, 674, 917, 1525, 1614, 1719
Bso31I	GGTCTC	1 cut(s)	279
BsoBI	CYCGRG	2 cut(s)	196, 1290
Bsp1286I	GDGCHC	4 cut(s)	205, 996, 1261, 1291
Bsp143I	GATC	8 cut(s)	109, 484, 796, 820, 880, 940, 1093, 1111
BspACI	CCGC	2 cut(s)	60, 302
BspANI	GGCC	6 cut(s)	289, 674, 917, 1525, 1614, 1719
BspCNI	CTCAG	5 cut(s)	223, 229, 1256, 1263, 1658
BspHI	TCATGA	1 cut(s)	558
BspLI	GGNNCC	2 cut(s)	1392, 1629
BspMAI	CTGCAG	1 cut(s)	424
BspMI	ACCTGC	1 cut(s)	422
BspOI	GCTAGC	1 cut(s)	1190
BspPI	GGATC	4 cut(s)	804, 875, 948, 1119
BspT107I	GGYRCC	1 cut(s)	1390
BspTNI	GGTCTC	1 cut(s)	279
BsrFI	RCCGGY	2 cut(s)	337, 1236
BsrI	ACTGG	3 cut(s)	850, 942, 1533
BssAI	RCCGGY	2 cut(s)	337, 1236
BssECI	CCNNGG	2 cut(s)	280, 636
BssMI	GATC	8 cut(s)	109, 484, 796, 820, 880, 940, 1093, 1111
BssNI	GRCGYC	1 cut(s)	17
BssT1I	CCWWGG	1 cut(s)	636
Bst2UI	CCWGG	5 cut(s)	215, 282, 885, 1522, 1716
Bst4CI	ACNGT	3 cut(s)	273, 718, 1637
Bst6I	CTCTTC	1 cut(s)	1407
BstACI	GRCGYC	1 cut(s)	17
BstAPI	GCANNNNNTGC	1 cut(s)	1286
BstBAI	YACGTR	1 cut(s)	1193
BstC8I	GCNNGC	5 cut(s)	205, 209, 339, 955, 1188
BstDEI	CTNAG	8 cut(s)	231, 237, 1243, 1271, 1344, 1427, 1645, 1733
BstF5I	GGATG	2 cut(s)	643, 1188
BstKTI	GATC	8 cut(s)	112, 487, 799, 823, 883, 943, 1096, 1114
BstMAI	GTCTC	5 cut(s)	279, 444, 928, 1221, 1267
BstMBI	GATC	8 cut(s)	109, 484, 796, 820, 880, 940, 1093, 1111
BstMWI	GCNNNNNNNGC	3 cut(s)	316, 428, 1286
BstNI	CCWGG	5 cut(s)	215, 282, 885, 1522, 1716
BstSCI	CCNGG	5 cut(s)	213, 280, 883, 1520, 1714
BstSFI	CTRYAG	1 cut(s)	420
BstSLI	GKGCMC	2 cut(s)	1261, 1291
BstV1I	GCAGC	2 cut(s)	120, 331
BstV2I	GAAGAC	1 cut(s)	591
BstX2I	RGATCY	3 cut(s)	796, 880, 1093
BstXI	CCANNNNNNTGG	2 cut(s)	46, 845
BstYI	RGATCY	3 cut(s)	796, 880, 1093
BsuI	GTATCC	1 cut(s)	56
BsuRI	GGCC	6 cut(s)	289, 674, 917, 1525, 1614, 1719
BtrI	CACGTC	1 cut(s)	1277
BtsCI	GGATG	2 cut(s)	643, 1188
BtsIMutI	CAGTG	3 cut(s)	843, 1642, 1758
BveI	ACCTGC	1 cut(s)	422
Cac8I	GCNNGC	5 cut(s)	205, 209, 339, 955, 1188
CciI	TCATGA	1 cut(s)	558
Cfr10I	RCCGGY	2 cut(s)	337, 1236
Cfr13I	GGNCC	3 cut(s)	1477, 1628, 1772
CseI	GACGC	1 cut(s)	25
Csp6I	GTAC	5 cut(s)	395, 445, 1240, 1391, 1536
CspAI	ACCGGT	1 cut(s)	1236
CspCI	CAANNNNNGTGG	2 cut(s)	450, 485
CviAII	CATG	7 cut(s)	559, 802, 839, 1021, 1088, 1162, 1585
CviQI	GTAC	5 cut(s)	395, 445, 1240, 1391, 1536
DdeI	CTNAG	8 cut(s)	231, 237, 1243, 1271, 1344, 1427, 1645, 1733
DpnI	GATC	8 cut(s)	111, 486, 798, 822, 882, 942, 1095, 1113
DpnII	GATC	8 cut(s)	109, 484, 796, 820, 880, 940, 1093, 1111
EaeI	YGGCCR	2 cut(s)	287, 672
Eam1104I	CTCTTC	1 cut(s)	1407
EarI	CTCTTC	1 cut(s)	1407
Ecl136II	GAGCTC	2 cut(s)	203, 994
Eco130I	CCWWGG	1 cut(s)	636
Eco147I	AGGCCT	1 cut(s)	1614
Eco24I	GRGCYC	2 cut(s)	205, 996
Eco31I	GGTCTC	1 cut(s)	279
Eco32I	GATATC	1 cut(s)	1122
Eco47I	GGWCC	3 cut(s)	1477, 1628, 1772
Eco53kI	GAGCTC	2 cut(s)	203, 994
Eco57I	CTGAAG	5 cut(s)	56, 246, 945, 1601, 1734
Eco72I	CACGTG	1 cut(s)	1193
Eco88I	CYCGRG	2 cut(s)	196, 1290
EcoICRI	GAGCTC	2 cut(s)	203, 994
EcoRII	CCWGG	5 cut(s)	213, 280, 883, 1520, 1714
EcoRV	GATATC	1 cut(s)	1122
EcoT14I	CCWWGG	1 cut(s)	636
EcoT38I	GRGCYC	2 cut(s)	205, 996
ErhI	CCWWGG	1 cut(s)	636
Esp3I	CGTCTC	2 cut(s)	1221, 1267
FaeI	CATG	7 cut(s)	562, 805, 842, 1024, 1091, 1165, 1588
FalI	AAGNNNNNCTT	4 cut(s)	1531, 1563, 1543, 1575
FaqI	GGGAC	1 cut(s)	1641
FatI	CATG	7 cut(s)	558, 801, 838, 1020, 1087, 1161, 1584
FauI	CCCGC	1 cut(s)	295
Fnu4HI	GCNGC	2 cut(s)	134, 320
FokI	GGATG	2 cut(s)	630, 1195
FriOI	GRGCYC	2 cut(s)	205, 996
Fsp4HI	GCNGC	2 cut(s)	134, 320
FspBI	CTAG	7 cut(s)	503, 728, 1146, 1187, 1224, 1400, 1610
GluI	GCNGC	2 cut(s)	134, 320
GsaI	CCCAGC	2 cut(s)	266, 1316
GsuI	CTGGAG	1 cut(s)	179
HaeIII	GGCC	6 cut(s)	289, 674, 917, 1525, 1614, 1719
HapII	CCGG	2 cut(s)	338, 1237
HgaI	GACGC	1 cut(s)	25
Hin1I	GRCGYC	1 cut(s)	17
Hin1II	CATG	7 cut(s)	562, 805, 842, 1024, 1091, 1165, 1588
HincII	GTYRAC	2 cut(s)	1303, 1761
HindII	GTYRAC	2 cut(s)	1303, 1761
HindIII	AAGCTT	3 cut(s)	649, 758, 1140
HinfI	GANTC	4 cut(s)	326, 564, 722, 750
HpaII	CCGG	2 cut(s)	338, 1237
HphI	GGTGA	5 cut(s)	235, 388, 484, 625, 755
Hpy166II	GTNNAC	7 cut(s)	243, 589, 1259, 1303, 1642, 1761, 1775
Hpy188I	TCNGA	6 cut(s)	15, 411, 442, 516, 880, 1042
Hpy188III	TCNNGA	7 cut(s)	74, 158, 198, 559, 931, 979, 1125
Hpy8I	GTNNAC	7 cut(s)	243, 589, 1259, 1303, 1642, 1761, 1775
HpyAV	CCTTC	8 cut(s)	121, 169, 1468, 1593, 1625, 1666, 1670, 1678
HpyCH4III	ACNGT	3 cut(s)	273, 718, 1637
HpyCH4IV	ACGT	4 cut(s)	1192, 1276, 1285, 1763
HpyF10VI	GCNNNNNNNGC	3 cut(s)	316, 428, 1286
HpyF3I	CTNAG	8 cut(s)	231, 237, 1243, 1271, 1344, 1427, 1645, 1733
HpySE526I	ACGT	4 cut(s)	1192, 1276, 1285, 1763
Hsp92I	GRCGYC	1 cut(s)	17
Hsp92II	CATG	7 cut(s)	562, 805, 842, 1024, 1091, 1165, 1588
KpnI	GGTACC	1 cut(s)	1394
KroI	GCCGGC	1 cut(s)	337
KroNI	GCCGGC	1 cut(s)	339
Kzo9I	GATC	8 cut(s)	109, 484, 796, 820, 880, 940, 1093, 1111
LmnI	GCTCC	3 cut(s)	41, 520, 1201
Lsp1109I	GCAGC	2 cut(s)	120, 331
LweI	GCATC	2 cut(s)	571, 1173
MaeI	CTAG	7 cut(s)	503, 728, 1146, 1187, 1224, 1400, 1610
MaeII	ACGT	4 cut(s)	1192, 1276, 1285, 1763
MaeIII	GTNAC	2 cut(s)	841, 1193
MalI	GATC	8 cut(s)	111, 486, 798, 822, 882, 942, 1095, 1113
MboI	GATC	8 cut(s)	109, 484, 796, 820, 880, 940, 1093, 1111
MfeI	CAATTG	1 cut(s)	1622
MflI	RGATCY	3 cut(s)	796, 880, 1093
MhlI	GDGCHC	4 cut(s)	205, 996, 1261, 1291
MlsI	TGGCCA	1 cut(s)	674
MluCI	AATT	8 cut(s)	77, 296, 405, 414, 592, 659, 1026, 1622
MluNI	TGGCCA	1 cut(s)	674
MlyI	GAGTC	2 cut(s)	573, 744
MmeI	TCCRAC	2 cut(s)	1191, 1389
Mox20I	TGGCCA	1 cut(s)	674
MroNI	GCCGGC	1 cut(s)	337
MroXI	GAANNNNTTC	3 cut(s)	759, 1373, 1603
MscI	TGGCCA	1 cut(s)	674
MseI	TTAA	3 cut(s)	708, 947, 1059
Msp20I	TGGCCA	1 cut(s)	674
MspA1I	CMGCKG	1 cut(s)	322
MspI	CCGG	2 cut(s)	338, 1237
MspR9I	CCNGG	5 cut(s)	215, 282, 885, 1522, 1716
MunI	CAATTG	1 cut(s)	1622
Mva1269I	GAATGC	1 cut(s)	33
MvaI	CCWGG	5 cut(s)	215, 282, 885, 1522, 1716
MwoI	GCNNNNNNNGC	3 cut(s)	316, 428, 1286
NaeI	GCCGGC	1 cut(s)	339
NdeII	GATC	8 cut(s)	109, 484, 796, 820, 880, 940, 1093, 1111
NgoMIV	GCCGGC	1 cut(s)	337
NheI	GCTAGC	1 cut(s)	1186
NlaIII	CATG	7 cut(s)	562, 805, 842, 1024, 1091, 1165, 1588
NlaIV	GGNNCC	2 cut(s)	1392, 1629
NmuCI	GTSAC	2 cut(s)	841, 1193
PaeR7I	CTCGAG	1 cut(s)	196
PagI	TCATGA	1 cut(s)	558
PceI	AGGCCT	1 cut(s)	1614
PcsI	WCGNNNNNNNCGW	1 cut(s)	1282
PctI	GAATGC	1 cut(s)	33
PdiI	GCCGGC	1 cut(s)	339
PdmI	GAANNNNTTC	3 cut(s)	759, 1373, 1603
PfeI	GAWTC	2 cut(s)	326, 722
PflMI	CCANNNNNTGG	1 cut(s)	220
PfoI	TCCNGGA	1 cut(s)	883
PinAI	ACCGGT	1 cut(s)	1236
PkrI	GCNGC	2 cut(s)	135, 321
PleI	GAGTC	2 cut(s)	572, 744
PmaCI	CACGTG	1 cut(s)	1193
PmlI	CACGTG	1 cut(s)	1193
PpsI	GAGTC	2 cut(s)	572, 744
Ppu21I	YACGTR	1 cut(s)	1193
Psp124BI	GAGCTC	2 cut(s)	205, 996
Psp1406I	AACGTT	1 cut(s)	1763
Psp6I	CCWGG	5 cut(s)	213, 280, 883, 1520, 1714
PspCI	CACGTG	1 cut(s)	1193
PspFI	CCCAGC	2 cut(s)	262, 1312
PspGI	CCWGG	5 cut(s)	213, 280, 883, 1520, 1714
PspN4I	GGNNCC	2 cut(s)	1392, 1629
PspPI	GGNCC	3 cut(s)	1477, 1628, 1772
PstI	CTGCAG	1 cut(s)	424
PsuI	RGATCY	3 cut(s)	796, 880, 1093
PvuII	CAGCTG	1 cut(s)	322
RsaI	GTAC	5 cut(s)	396, 446, 1241, 1392, 1537
RsaNI	GTAC	5 cut(s)	395, 445, 1240, 1391, 1536
SacI	GAGCTC	2 cut(s)	205, 996
SaqAI	TTAA	3 cut(s)	708, 947, 1059
SatI	GCNGC	2 cut(s)	134, 320
Sau3AI	GATC	8 cut(s)	109, 484, 796, 820, 880, 940, 1093, 1111
Sau96I	GGNCC	3 cut(s)	1477, 1628, 1772
SchI	GAGTC	2 cut(s)	573, 744
ScrFI	CCNGG	5 cut(s)	215, 282, 885, 1522, 1716
SduI	GDGCHC	4 cut(s)	205, 996, 1261, 1291
SfaNI	GCATC	2 cut(s)	571, 1173
SfcI	CTRYAG	1 cut(s)	420
Sfr274I	CTCGAG	1 cut(s)	196
SinI	GGWCC	3 cut(s)	1477, 1628, 1772
SlaI	CTCGAG	1 cut(s)	196
SmlI	CTYRAG	3 cut(s)	196, 995, 1653
SmoI	CTYRAG	3 cut(s)	196, 995, 1653
Sse9I	AATT	8 cut(s)	77, 296, 405, 414, 592, 659, 1026, 1622
SseBI	AGGCCT	1 cut(s)	1614
SsiI	CCGC	2 cut(s)	60, 302
SspI	AATATT	1 cut(s)	694
SspMI	CTAG	7 cut(s)	503, 728, 1146, 1187, 1224, 1400, 1610
SstI	GAGCTC	2 cut(s)	205, 996
StuI	AGGCCT	1 cut(s)	1614
StyD4I	CCNGG	5 cut(s)	213, 280, 883, 1520, 1714
StyI	CCWWGG	1 cut(s)	636
TaaI	ACNGT	3 cut(s)	273, 718, 1637
TaiI	ACGT	4 cut(s)	1195, 1279, 1288, 1766
TaqI	TCGA	6 cut(s)	197, 388, 567, 602, 753, 1711
TasI	AATT	8 cut(s)	77, 296, 405, 414, 592, 659, 1026, 1622
TatI	WGTACW	2 cut(s)	394, 1535
TfiI	GAWTC	2 cut(s)	326, 722
Tru1I	TTAA	3 cut(s)	708, 947, 1059
Tru9I	TTAA	3 cut(s)	708, 947, 1059
TscAI	CASTG	3 cut(s)	850, 1642, 1758
TseFI	GTSAC	2 cut(s)	841, 1193
TseI	GCWGC	2 cut(s)	133, 319
Tsp45I	GTSAC	2 cut(s)	841, 1193
TspDTI	ATGAA	8 cut(s)	111, 564, 632, 672, 977, 1504, 1685, 1755
TspGWI	ACGGA	2 cut(s)	462, 533
TspRI	CASTG	3 cut(s)	850, 1642, 1758
Van91I	CCANNNNNTGG	1 cut(s)	220
VneI	GTGCAC	1 cut(s)	1257
VpaK11BI	GGWCC	3 cut(s)	1477, 1628, 1772
XapI	RAATTY	3 cut(s)	405, 414, 1026
XhoI	CTCGAG	1 cut(s)	196
XmnI	GAANNNNTTC	3 cut(s)	759, 1373, 1603
XspI	CTAG	7 cut(s)	503, 728, 1146, 1187, 1224, 1400, 1610

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.