AT1G30380

Photosystem I reaction center subunit psaK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0015469
Physical Location & Seq
Forward (+)
10722183 .. 10723247
1065 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G30380.1

Sequence Viewer

Length: 393 bp
ATGGCTAGCACTATGATGACTACATTGCCTCAGTTCAATGGTCTTCGAGCCACCAAAATCTCTGCAGCTCCTGTACAAGGCCTGGCAAGTGTTCAGCCCATGAGACGCAAGGGAAATGGAGCTTTGGGTGCAAAGTGTGACTTCATCGGTTCATCAACAAATCTGATAATGGTAACGTCGACGACCCTGATGTTGTTCGCGGGGAGATTCGGACTTGCGCCATCAGCCAATAGGAAGGCGACAGCTGGACTTAGGTTGGAGGCACGTGACTCAGGTCTACAAACGGGTGACCCGGCCGGGTTCACGCTTGCGGACACTTTGGCTTGTGGCACCGTTGGTCATATCATCGGTGTAGGAGTTGTCCTTGGCCTTAAAAACATTGGTGCTATTTGA

Protein Analysis

130

Amino Acids

13.21

Weight (kDa)

10.52

Isoelectric Point (pI)

31.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PSAK PF01241 52 - 128 7.5e-19 Photosystem I psaG / psaK
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30380
fragaria_vesca FvH4_3g09680
malus_domestica MD00G1109700.v1.1 MD05G1276100.v1.1
prunus_persica Prupe.4G088200_v2.0.a1
pyrus_communis pycom05g25710 pycom10g21120
rosa_chinensis RchiOBHm_Chr5g0015361
rosa_laevigata RLG00000032195
rosa_multiflora Rmu_sc0014430.1_g000001
rosa_rugosa Rorug05G0026800
rosa_samantha Rh5AG120400 Rh5BG119400 Rh5CG129800 Rh5DG118400
rosa_wichuraiana Rw5G010410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 329
AccI GTMKAC 2 cut(s) 179, 277
AccII CGCG 1 cut(s) 200
AciI CCGC 2 cut(s) 200, 311
AcoI YGGCCR 1 cut(s) 294
AcvI CACGTG 1 cut(s) 266
AfaI GTAC 1 cut(s) 75
AfiI CCNNNNNNNGG 1 cut(s) 77
AgsI TTSAA 1 cut(s) 37
AjnI CCWGG 1 cut(s) 81
AluBI AGCT 3 cut(s) 68, 122, 245
AluI AGCT 3 cut(s) 68, 122, 245
Alw26I GTCTC 1 cut(s) 97
AlwNI CAGNNNCTG 1 cut(s) 71
AoxI GGCC 3 cut(s) 79, 294, 367
ApeKI GCWGC 1 cut(s) 65
AspLEI GCGC 1 cut(s) 220
AsuC2I CCSGG 2 cut(s) 293, 298
AsuHPI GGTGA 1 cut(s) 299
AsuNHI GCTAGC 1 cut(s) 5
BanI GGYRCC 1 cut(s) 329
BbrPI CACGTG 1 cut(s) 266
BbsI GAAGAC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 77
BccI CCATC 1 cut(s) 229
BciT130I CCWGG 1 cut(s) 83
BcnI CCSGG 2 cut(s) 293, 298
BcoDI GTCTC 1 cut(s) 97
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 63
BisI GCNGC 1 cut(s) 66
BlsI GCNGC 1 cut(s) 67
Bme1390I CCNGG 3 cut(s) 83, 293, 298
BmiI GGNNCC 1 cut(s) 331
BmrFI CCNGG 3 cut(s) 83, 293, 298
BmtI GCTAGC 1 cut(s) 9
BoxI GACNNNNGTC 1 cut(s) 273
BpiI GAAGAC 1 cut(s) 35
BpuMI CCSGG 2 cut(s) 293, 298
BsaAI YACGTR 1 cut(s) 266
BsaJI CCNNGG 1 cut(s) 364
Bsc4I CCNNNNNNNGG 1 cut(s) 77
Bse3DI GCAATG 1 cut(s) 23
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 1 cut(s) 364
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMI GCAATG 1 cut(s) 23
BseMII CTCAG 2 cut(s) 44, 285
BseX3I CGGCCG 1 cut(s) 294
BseXI GCAGC 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 200
Bsh1285I CGRYCG 1 cut(s) 297
BshFI GGCC 3 cut(s) 81, 296, 369
BshNI GGYRCC 1 cut(s) 329
BsiEI CGRYCG 1 cut(s) 297
BsiSI CCGG 2 cut(s) 293, 297
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 97
BsmBI CGTCTC 1 cut(s) 97
BsnI GGCC 3 cut(s) 81, 296, 369
Bsp1407I TGTACA 1 cut(s) 73
BspACI CCGC 2 cut(s) 200, 311
BspANI GGCC 3 cut(s) 81, 296, 369
BspCNI CTCAG 2 cut(s) 43, 284
BspFNI CGCG 1 cut(s) 200
BspLI GGNNCC 1 cut(s) 331
BspMAI CTGCAG 1 cut(s) 67
BspOI GCTAGC 1 cut(s) 9
BspT107I GGYRCC 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 23
BsrGI TGTACA 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 364
BssT1I CCWWGG 1 cut(s) 364
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 334
BstAUI TGTACA 1 cut(s) 73
BstBAI YACGTR 1 cut(s) 266
BstC8I GCNNGC 2 cut(s) 7, 309
BstDEI CTNAG 3 cut(s) 30, 251, 271
BstEII GGTNACC 1 cut(s) 287
BstENI CCTNNNNNAGG 1 cut(s) 75
BstFNI CGCG 1 cut(s) 200
BstHHI GCGC 1 cut(s) 220
BstMAI GTCTC 1 cut(s) 97
BstMCI CGRYCG 1 cut(s) 297
BstMWI GCNNNNNNNGC 2 cut(s) 128, 224
BstNI CCWGG 1 cut(s) 83
BstPAI GACNNNNGTC 1 cut(s) 273
BstPI GGTNACC 1 cut(s) 287
BstSCI CCNGG 3 cut(s) 81, 291, 296
BstSFI CTRYAG 1 cut(s) 63
BstUI CGCG 1 cut(s) 200
BstV1I GCAGC 1 cut(s) 77
BstV2I GAAGAC 1 cut(s) 35
BstZI CGGCCG 1 cut(s) 294
BsuRI GGCC 3 cut(s) 81, 296, 369
Cac8I GCNNGC 2 cut(s) 7, 309
CaiI CAGNNNCTG 1 cut(s) 71
CfoI GCGC 1 cut(s) 220
CseI GACGC 1 cut(s) 114
Csp6I GTAC 1 cut(s) 74
CviAII CATG 1 cut(s) 100
CviQI GTAC 1 cut(s) 74
DdeI CTNAG 3 cut(s) 30, 251, 271
EaeI YGGCCR 1 cut(s) 294
EagI CGGCCG 1 cut(s) 294
EclXI CGGCCG 1 cut(s) 294
Eco130I CCWWGG 1 cut(s) 364
Eco147I AGGCCT 1 cut(s) 81
Eco52I CGGCCG 1 cut(s) 294
Eco72I CACGTG 1 cut(s) 266
Eco91I GGTNACC 1 cut(s) 287
EcoNI CCTNNNNNAGG 1 cut(s) 75
EcoO65I GGTNACC 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 364
ErhI CCWWGG 1 cut(s) 364
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 1 cut(s) 103
FaiI YATR 3 cut(s) 14, 101, 342
FalI AAGNNNNNCTT 2 cut(s) 125, 157
FatI CATG 1 cut(s) 99
FauI CCCGC 1 cut(s) 193
FblI GTMKAC 2 cut(s) 179, 277
Fnu4HI GCNGC 1 cut(s) 66
Fsp4HI GCNGC 1 cut(s) 66
FspBI CTAG 1 cut(s) 6
GlaI GCGC 1 cut(s) 219
GluI GCNGC 1 cut(s) 66
HaeIII GGCC 3 cut(s) 81, 296, 369
HapII CCGG 2 cut(s) 293, 297
HgaI GACGC 1 cut(s) 114
HhaI GCGC 1 cut(s) 220
Hin1II CATG 1 cut(s) 103
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HincII GTYRAC 1 cut(s) 180
HindII GTYRAC 1 cut(s) 180
HinfI GANTC 2 cut(s) 207, 269
HpaII CCGG 2 cut(s) 293, 297
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 3 cut(s) 180, 278, 303
Hpy188I TCNGA 2 cut(s) 165, 212
Hpy8I GTNNAC 3 cut(s) 180, 278, 303
Hpy99I CGWCG 2 cut(s) 181, 184
HpyAV CCTTC 1 cut(s) 229
HpyCH4III ACNGT 1 cut(s) 334
HpyCH4IV ACGT 2 cut(s) 176, 265
HpyCH4V TGCA 2 cut(s) 65, 131
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 224
HpyF3I CTNAG 3 cut(s) 30, 251, 271
HpySE526I ACGT 2 cut(s) 176, 265
Hsp92II CATG 1 cut(s) 103
HspAI GCGC 1 cut(s) 218
LmnI GCTCC 2 cut(s) 73, 119
LpnPI CCDG 8 cut(s) 68, 84, 95, 200, 231, 258, 306, 310
Lsp1109I GCAGC 1 cut(s) 77
MaeI CTAG 1 cut(s) 6
MaeII ACGT 2 cut(s) 176, 265
MaeIII GTNAC 4 cut(s) 137, 172, 266, 287
MboII GAAGA 1 cut(s) 35
MlyI GAGTC 1 cut(s) 263
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 2 cut(s) 39, 253
MseI TTAA 1 cut(s) 372
MslI CAYNNNNRTG 1 cut(s) 14
MspA1I CMGCKG 1 cut(s) 245
MspI CCGG 2 cut(s) 293, 297
MspR9I CCNGG 3 cut(s) 83, 293, 298
MvaI CCWGG 1 cut(s) 83
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 2 cut(s) 128, 224
NciI CCSGG 2 cut(s) 293, 298
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 1 cut(s) 103
NlaIV GGNNCC 1 cut(s) 331
NmuCI GTSAC 3 cut(s) 137, 266, 287
PceI AGGCCT 1 cut(s) 81
PfeI GAWTC 1 cut(s) 207
PkrI GCNGC 1 cut(s) 67
PleI GAGTC 1 cut(s) 263
PmaCI CACGTG 1 cut(s) 266
PmlI CACGTG 1 cut(s) 266
PpsI GAGTC 1 cut(s) 263
Ppu21I YACGTR 1 cut(s) 266
PshAI GACNNNNGTC 1 cut(s) 273
Psp6I CCWGG 1 cut(s) 81
PspCI CACGTG 1 cut(s) 266
PspEI GGTNACC 1 cut(s) 287
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 1 cut(s) 331
PstI CTGCAG 1 cut(s) 67
PstNI CAGNNNCTG 1 cut(s) 71
PvuII CAGCTG 1 cut(s) 245
RsaI GTAC 1 cut(s) 75
RsaNI GTAC 1 cut(s) 74
RseI CAYNNNNRTG 1 cut(s) 14
SalI GTCGAC 1 cut(s) 178
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 1 cut(s) 66
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 3 cut(s) 83, 293, 298
SetI ASST 7 cut(s) 70, 124, 179, 247, 257, 268, 277
SfcI CTRYAG 1 cut(s) 63
SgrDI CGTCGACG 1 cut(s) 178
SmiMI CAYNNNNRTG 1 cut(s) 14
SseBI AGGCCT 1 cut(s) 81
SsiI CCGC 2 cut(s) 200, 311
SspMI CTAG 1 cut(s) 6
StuI AGGCCT 1 cut(s) 81
StyD4I CCNGG 3 cut(s) 81, 291, 296
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 1 cut(s) 334
TaiI ACGT 2 cut(s) 179, 268
TaqI TCGA 2 cut(s) 46, 179
TatI WGTACW 1 cut(s) 73
TfiI GAWTC 1 cut(s) 207
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TseFI GTSAC 3 cut(s) 137, 266, 287
TseI GCWGC 1 cut(s) 65
Tsp45I GTSAC 3 cut(s) 137, 266, 287
TspDTI ATGAA 2 cut(s) 133, 141
XagI CCTNNNNNAGG 1 cut(s) 75
XmiI GTMKAC 2 cut(s) 179, 277
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.