RLG00000032195

Photosystem I reaction center subunit psaK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015469
Physical Location & Seq
Forward (+)
11960119 .. 11962788
2670 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032195

Sequence Viewer

Length: 759 bp
ATGGAGTCCCTATCGGGTTTCGGCCTATACCTAGTTGTTATTGTCGCTTTCCACAGTAGAGGTGGCGGTGCGGTGGATGGCTGCGGTCGTCTGCAATTTCAAGGACGCTTGACAATGGCAACCTCGTCTAGAGGTTGGGCATCGTCAACGTCTTTCAGTTGGCCCGACCTCTTTCTTCGACTTCTCCATGTCGCTGGTGGACTGGCTATCAGAGATCTAGCCGACGGTGGTGCTGGGTCAGTGGCCATTTCTAAATCAGGGTCATTGTCCATTCTCAGCTCAATACCTGAAATTCCATCATCAATAATCCAAATGGACATTTCTGAAGCTCACAAAAACCTTATCCAACTGAGAAAATCCAATGAGAAAATGGCAGCAGCGACTGTGACTACTTCTCTCCCCCAGTTCAATGGGTTGAGACCCAAGATCTCATCCAATGTACAGAGCTTGGCTGCTGTTCAACCAATGAGGCGCAAGAGCCAAGGTGCCTTGGGAGCTCGCTGTGATTTCATCGGTTCACCCACCAATTTGATAATGGTGACTACTACAAGCCTAATGTTGTTTGCTGGAAGATTCGGGTTGGCGCCATCTGCAAACAGGAAGGCAACAGCAGGATTGAAGCTTGAAATGAGGGACTCAGGGCTTCAGACCGGTGACCCAGCTGGGTTCACCCTTGCTGATACCTTGGCTTGTGGGAGTGTAGGTCACATCATTGGGGTTGGGGTTGTTCTTGGCCTTAAGAATATAGGTGCTCTGTAA

Protein Analysis

253

Amino Acids

26.13

Weight (kDa)

10.02

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PSAK PF01241 175 - 250 4.9e-18 Photosystem I psaG / psaK
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30380
fragaria_vesca FvH4_3g09680
malus_domestica MD00G1109700.v1.1 MD05G1276100.v1.1
prunus_persica Prupe.4G088200_v2.0.a1
pyrus_communis pycom05g25710 pycom10g21120
rosa_chinensis RchiOBHm_Chr5g0015361
rosa_laevigata RLG00000032195
rosa_multiflora Rmu_sc0014430.1_g000001
rosa_rugosa Rorug05G0026800
rosa_samantha Rh5AG120400 Rh5BG119400 Rh5CG129800 Rh5DG118400
rosa_wichuraiana Rw5G010410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 485, 583
AciI CCGC 3 cut(s) 66, 71, 84
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 1 cut(s) 291
AcuI CTGAAG 2 cut(s) 345, 629
AcyI GRCGYC 1 cut(s) 584
AfaI GTAC 1 cut(s) 441
AflII CTTAAG 1 cut(s) 737
AgeI ACCGGT 1 cut(s) 650
AgsI TTSAA 5 cut(s) 101, 409, 461, 619, 626
AluBI AGCT 6 cut(s) 279, 329, 447, 497, 622, 662
AluI AGCT 6 cut(s) 279, 329, 447, 497, 622, 662
Alw21I GWGCWC 2 cut(s) 499, 754
Alw26I GTCTC 1 cut(s) 412
AlwNI CAGNNNCTG 1 cut(s) 383
AoxI GGCC 4 cut(s) 22, 161, 243, 733
ApeKI GCWGC 4 cut(s) 81, 374, 377, 452
ApoI RAATTY 1 cut(s) 291
AsiGI ACCGGT 1 cut(s) 650
AspLEI GCGC 2 cut(s) 474, 586
AspS9I GGNCC 1 cut(s) 162
AsuHPI GGTGA 4 cut(s) 510, 550, 661, 665
BalI TGGCCA 1 cut(s) 245
BanI GGYRCC 2 cut(s) 485, 583
BanII GRGCYC 1 cut(s) 499
Bbv12I GWGCWC 2 cut(s) 499, 754
BbvI GCAGC 4 cut(s) 68, 386, 389, 439
BccI CCATC 3 cut(s) 71, 304, 595
BcgI CGANNNNNNTGC 2 cut(s) 212, 246
BcoDI GTCTC 1 cut(s) 412
BfaI CTAG 3 cut(s) 32, 129, 218
BfoI RGCGCY 1 cut(s) 587
BfrI CTTAAG 1 cut(s) 737
BglII AGATCT 2 cut(s) 214, 426
BisI GCNGC 4 cut(s) 82, 375, 378, 453
BlsI GCNGC 4 cut(s) 83, 376, 379, 454
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 2 cut(s) 487, 585
BmrI ACTGGG 1 cut(s) 397
BmsI GCATC 1 cut(s) 149
BmuI ACTGGG 1 cut(s) 397
BsaHI GRCGYC 1 cut(s) 584
BsaI GGTCTC 1 cut(s) 412
BsaJI CCNNGG 3 cut(s) 481, 489, 684
BsaWI WCCGGW 1 cut(s) 650
BsaXI ACNNNNNCTCC 2 cut(s) 688, 718
Bse118I RCCGGY 1 cut(s) 650
Bse1I ACTGG 2 cut(s) 207, 403
BseDI CCNNGG 3 cut(s) 481, 489, 684
BseGI GGATG 2 cut(s) 82, 431
BseMII CTCAG 3 cut(s) 289, 341, 651
BseNI ACTGG 2 cut(s) 207, 403
BseXI GCAGC 4 cut(s) 68, 386, 389, 439
BseYI CCCAGC 3 cut(s) 233, 658, 662
Bsh1285I CGRYCG 1 cut(s) 88
BshFI GGCC 4 cut(s) 24, 163, 245, 735
BshNI GGYRCC 2 cut(s) 485, 583
BshTI ACCGGT 1 cut(s) 650
BsiEI CGRYCG 1 cut(s) 88
BsiHKAI GWGCWC 2 cut(s) 499, 754
BsiSI CCGG 1 cut(s) 651
BslFI GGGAC 1 cut(s) 647
BsmAI GTCTC 1 cut(s) 412
BsmFI GGGAC 1 cut(s) 647
BsnI GGCC 4 cut(s) 24, 163, 245, 735
Bso31I GGTCTC 1 cut(s) 412
Bsp1286I GDGCHC 2 cut(s) 499, 754
Bsp1407I TGTACA 1 cut(s) 439
Bsp143I GATC 2 cut(s) 214, 426
BspACI CCGC 3 cut(s) 66, 71, 84
BspANI GGCC 4 cut(s) 24, 163, 245, 735
BspCNI CTCAG 3 cut(s) 288, 342, 650
BspLI GGNNCC 2 cut(s) 487, 585
BspT107I GGYRCC 2 cut(s) 485, 583
BspTI CTTAAG 1 cut(s) 737
BspTNI GGTCTC 1 cut(s) 412
BsrFI RCCGGY 1 cut(s) 650
BsrGI TGTACA 1 cut(s) 439
BsrI ACTGG 2 cut(s) 207, 403
BssAI RCCGGY 1 cut(s) 650
BssECI CCNNGG 3 cut(s) 481, 489, 684
BssMI GATC 2 cut(s) 214, 426
BssNI GRCGYC 1 cut(s) 584
BssT1I CCWWGG 3 cut(s) 481, 489, 684
Bst4CI ACNGT 3 cut(s) 56, 227, 385
BstACI GRCGYC 1 cut(s) 584
BstAFI CTTAAG 1 cut(s) 737
BstAUI TGTACA 1 cut(s) 439
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 3 cut(s) 275, 350, 637
BstEII GGTNACC 1 cut(s) 653
BstF5I GGATG 2 cut(s) 82, 431
BstH2I RGCGCY 1 cut(s) 587
BstHHI GCGC 2 cut(s) 474, 586
BstKTI GATC 2 cut(s) 217, 429
BstMAI GTCTC 1 cut(s) 412
BstMBI GATC 2 cut(s) 214, 426
BstMCI CGRYCG 1 cut(s) 88
BstMWI GCNNNNNNNGC 2 cut(s) 494, 590
BstPI GGTNACC 1 cut(s) 653
BstV1I GCAGC 4 cut(s) 68, 386, 389, 439
BstX2I RGATCY 2 cut(s) 214, 426
BstXI CCANNNNNNTGG 2 cut(s) 194, 410
BstYI RGATCY 2 cut(s) 214, 426
BsuRI GGCC 4 cut(s) 24, 163, 245, 735
BtsCI GGATG 2 cut(s) 82, 431
BtsIMutI CAGTG 1 cut(s) 246
Cac8I GCNNGC 1 cut(s) 499
CaiI CAGNNNCTG 1 cut(s) 383
CfoI GCGC 2 cut(s) 474, 586
Cfr10I RCCGGY 1 cut(s) 650
Cfr13I GGNCC 1 cut(s) 162
CseI GACGC 1 cut(s) 114
Csp6I GTAC 1 cut(s) 440
CspAI ACCGGT 1 cut(s) 650
CviAII CATG 1 cut(s) 188
CviQI GTAC 1 cut(s) 440
DdeI CTNAG 3 cut(s) 275, 350, 637
DinI GGCGCC 1 cut(s) 585
DpnI GATC 2 cut(s) 216, 428
DpnII GATC 2 cut(s) 214, 426
EaeI YGGCCR 1 cut(s) 243
Ecl136II GAGCTC 1 cut(s) 497
Eco130I CCWWGG 3 cut(s) 481, 489, 684
Eco24I GRGCYC 1 cut(s) 499
Eco31I GGTCTC 1 cut(s) 412
Eco53kI GAGCTC 1 cut(s) 497
Eco57I CTGAAG 2 cut(s) 345, 629
Eco91I GGTNACC 1 cut(s) 653
EcoICRI GAGCTC 1 cut(s) 497
EcoO65I GGTNACC 1 cut(s) 653
EcoT14I CCWWGG 3 cut(s) 481, 489, 684
EcoT38I GRGCYC 1 cut(s) 499
EgeI GGCGCC 1 cut(s) 585
EheI GGCGCC 1 cut(s) 585
ErhI CCWWGG 3 cut(s) 481, 489, 684
FaeI CATG 1 cut(s) 191
FaiI YATR 3 cut(s) 28, 189, 746
FaqI GGGAC 1 cut(s) 647
FatI CATG 1 cut(s) 187
Fnu4HI GCNGC 4 cut(s) 82, 375, 378, 453
FokI GGATG 2 cut(s) 89, 418
FriOI GRGCYC 1 cut(s) 499
Fsp4HI GCNGC 4 cut(s) 82, 375, 378, 453
FspBI CTAG 3 cut(s) 32, 129, 218
GlaI GCGC 2 cut(s) 473, 585
GluI GCNGC 4 cut(s) 82, 375, 378, 453
GsaI CCCAGC 3 cut(s) 237, 662, 666
HaeII RGCGCY 1 cut(s) 587
HaeIII GGCC 4 cut(s) 24, 163, 245, 735
HapII CCGG 1 cut(s) 651
HgaI GACGC 1 cut(s) 114
HhaI GCGC 2 cut(s) 474, 586
Hin1I GRCGYC 1 cut(s) 584
Hin1II CATG 1 cut(s) 191
Hin6I GCGC 2 cut(s) 472, 584
HinP1I GCGC 2 cut(s) 472, 584
HincII GTYRAC 1 cut(s) 147
HindII GTYRAC 1 cut(s) 147
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 3 cut(s) 5, 573, 635
HpaII CCGG 1 cut(s) 651
HphI GGTGA 4 cut(s) 510, 550, 661, 665
Hpy166II GTNNAC 4 cut(s) 147, 200, 518, 669
Hpy188I TCNGA 3 cut(s) 212, 325, 648
Hpy188III TCNNGA 1 cut(s) 129
Hpy8I GTNNAC 4 cut(s) 147, 200, 518, 669
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 1 cut(s) 595
HpyCH4III ACNGT 3 cut(s) 56, 227, 385
HpyCH4IV ACGT 1 cut(s) 149
HpyCH4V TGCA 2 cut(s) 94, 593
HpyF10VI GCNNNNNNNGC 2 cut(s) 494, 590
HpyF3I CTNAG 3 cut(s) 275, 350, 637
HpySE526I ACGT 1 cut(s) 149
Hsp92I GRCGYC 1 cut(s) 584
Hsp92II CATG 1 cut(s) 191
HspAI GCGC 2 cut(s) 472, 584
KasI GGCGCC 1 cut(s) 583
Kzo9I GATC 2 cut(s) 214, 426
LmnI GCTCC 1 cut(s) 494
Lsp1109I GCAGC 4 cut(s) 68, 386, 389, 439
LweI GCATC 1 cut(s) 149
MaeI CTAG 3 cut(s) 32, 129, 218
MaeII ACGT 1 cut(s) 149
MaeIII GTNAC 4 cut(s) 385, 538, 653, 704
MalI GATC 2 cut(s) 216, 428
MboI GATC 2 cut(s) 214, 426
MboII GAAGA 2 cut(s) 167, 582
MflI RGATCY 2 cut(s) 214, 426
MhlI GDGCHC 2 cut(s) 499, 754
MlsI TGGCCA 1 cut(s) 245
MluCI AATT 3 cut(s) 95, 291, 526
MluNI TGGCCA 1 cut(s) 245
Mly113I GGCGCC 1 cut(s) 584
MlyI GAGTC 2 cut(s) 14, 629
MmeI TCCRAC 1 cut(s) 370
MnlI CCTC 6 cut(s) 53, 125, 133, 179, 462, 624
Mox20I TGGCCA 1 cut(s) 245
MscI TGGCCA 1 cut(s) 245
MseI TTAA 1 cut(s) 738
Msp20I TGGCCA 1 cut(s) 245
MspA1I CMGCKG 1 cut(s) 662
MspCI CTTAAG 1 cut(s) 737
MspI CCGG 1 cut(s) 651
MwoI GCNNNNNNNGC 2 cut(s) 494, 590
NarI GGCGCC 1 cut(s) 584
NdeII GATC 2 cut(s) 214, 426
NlaIII CATG 1 cut(s) 191
NlaIV GGNNCC 2 cut(s) 487, 585
NmuCI GTSAC 4 cut(s) 385, 538, 653, 704
PfeI GAWTC 1 cut(s) 573
PinAI ACCGGT 1 cut(s) 650
PkrI GCNGC 4 cut(s) 83, 376, 379, 454
PleI GAGTC 2 cut(s) 13, 629
PluTI GGCGCC 1 cut(s) 587
PpsI GAGTC 2 cut(s) 13, 629
Psp124BI GAGCTC 1 cut(s) 499
PspEI GGTNACC 1 cut(s) 653
PspFI CCCAGC 3 cut(s) 233, 658, 662
PspN4I GGNNCC 2 cut(s) 487, 585
PspPI GGNCC 1 cut(s) 162
PstNI CAGNNNCTG 1 cut(s) 383
PsuI RGATCY 2 cut(s) 214, 426
PvuII CAGCTG 1 cut(s) 662
RsaI GTAC 1 cut(s) 441
RsaNI GTAC 1 cut(s) 440
SacI GAGCTC 1 cut(s) 499
SaqAI TTAA 1 cut(s) 738
SatI GCNGC 4 cut(s) 82, 375, 378, 453
Sau3AI GATC 2 cut(s) 214, 426
Sau96I GGNCC 1 cut(s) 162
SchI GAGTC 2 cut(s) 14, 629
SduI GDGCHC 2 cut(s) 499, 754
SfaNI GCATC 1 cut(s) 149
SfoI GGCGCC 1 cut(s) 585
SmlI CTYRAG 1 cut(s) 737
SmoI CTYRAG 1 cut(s) 737
Sse9I AATT 3 cut(s) 95, 291, 526
SsiI CCGC 3 cut(s) 66, 71, 84
SspDI GGCGCC 1 cut(s) 583
SspMI CTAG 3 cut(s) 32, 129, 218
SstI GAGCTC 1 cut(s) 499
StyI CCWWGG 3 cut(s) 481, 489, 684
TaaI ACNGT 3 cut(s) 56, 227, 385
TaiI ACGT 1 cut(s) 152
TaqI TCGA 1 cut(s) 178
TasI AATT 3 cut(s) 95, 291, 526
TatI WGTACW 1 cut(s) 439
TfiI GAWTC 1 cut(s) 573
Tru1I TTAA 1 cut(s) 738
Tru9I TTAA 1 cut(s) 738
TscAI CASTG 1 cut(s) 246
TseFI GTSAC 4 cut(s) 385, 538, 653, 704
TseI GCWGC 4 cut(s) 81, 374, 377, 452
Tsp45I GTSAC 4 cut(s) 385, 538, 653, 704
TspDTI ATGAA 1 cut(s) 499
TspRI CASTG 1 cut(s) 246
Vha464I CTTAAG 1 cut(s) 737
XapI RAATTY 1 cut(s) 291
XbaI TCTAGA 1 cut(s) 128
XcmI CCANNNNNNNNNTGG 4 cut(s) 59, 194, 367, 532
XspI CTAG 3 cut(s) 32, 129, 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.