Rh5CG129800

Photosystem I reaction center subunit psaK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0015469
Physical Location & Seq
Forward (+)
10802599 .. 10804084
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG129800.1

Sequence Viewer

Length: 294 bp
ATGAGGCGCAAGAGCCAAGGTGCCTTGGGAGCTCGCTGTGATTTCATCGGTTCACCCACCAATTTGATAATGGTGACTACTACAAGCCTAATGTTGTTTGCTGGGAGATTCGGGTTGGCGCCATCTGCAAACAGGAAGGCAACAGCAGGATTGAAGCTTGAAATAAGGGACTCAGGGCTTCAGACCGGTGACCCAGCTGGGTTCACCCTTGCTGATACCTTGGCTTGTGGGACTGTGGGTCACATCATTGGGGTTGGGGTTGTTCTTGGCCTTAAGAACATTGGTGCTCTGTAA

Protein Analysis

97

Amino Acids

9.9

Weight (kDa)

10.04

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PSAK PF01241 20 - 95 5.5e-18 Photosystem I psaG / psaK
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30380
fragaria_vesca FvH4_3g09680
malus_domestica MD00G1109700.v1.1 MD05G1276100.v1.1
prunus_persica Prupe.4G088200_v2.0.a1
pyrus_communis pycom05g25710 pycom10g21120
rosa_chinensis RchiOBHm_Chr5g0015361
rosa_laevigata RLG00000032195
rosa_multiflora Rmu_sc0014430.1_g000001
rosa_rugosa Rorug05G0026800
rosa_samantha Rh5AG120400 Rh5BG119400 Rh5CG129800 Rh5DG118400
rosa_wichuraiana Rw5G010410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 20, 118
AcuI CTGAAG 1 cut(s) 164
AcyI GRCGYC 1 cut(s) 119
AflII CTTAAG 1 cut(s) 272
AgeI ACCGGT 1 cut(s) 185
AgsI TTSAA 2 cut(s) 154, 161
AhdI GACNNNNNGTC 1 cut(s) 237
AluBI AGCT 3 cut(s) 32, 157, 197
AluI AGCT 3 cut(s) 32, 157, 197
Alw21I GWGCWC 2 cut(s) 34, 289
AoxI GGCC 1 cut(s) 268
AsiGI ACCGGT 1 cut(s) 185
AspLEI GCGC 2 cut(s) 9, 121
AsuHPI GGTGA 4 cut(s) 45, 85, 196, 200
BanI GGYRCC 2 cut(s) 20, 118
BanII GRGCYC 1 cut(s) 34
Bbv12I GWGCWC 2 cut(s) 34, 289
BccI CCATC 1 cut(s) 130
BfoI RGCGCY 1 cut(s) 122
BfrI CTTAAG 1 cut(s) 272
BmeRI GACNNNNNGTC 1 cut(s) 237
BmiI GGNNCC 2 cut(s) 22, 120
BsaHI GRCGYC 1 cut(s) 119
BsaJI CCNNGG 3 cut(s) 16, 24, 219
BsaWI WCCGGW 1 cut(s) 185
Bse118I RCCGGY 1 cut(s) 185
BseDI CCNNGG 3 cut(s) 16, 24, 219
BseMII CTCAG 1 cut(s) 186
BseYI CCCAGC 3 cut(s) 101, 193, 197
BshFI GGCC 1 cut(s) 270
BshNI GGYRCC 2 cut(s) 20, 118
BshTI ACCGGT 1 cut(s) 185
BsiHKAI GWGCWC 2 cut(s) 34, 289
BsiSI CCGG 1 cut(s) 186
BslFI GGGAC 2 cut(s) 182, 244
BsmFI GGGAC 2 cut(s) 182, 244
BsnI GGCC 1 cut(s) 270
Bsp1286I GDGCHC 2 cut(s) 34, 289
BspANI GGCC 1 cut(s) 270
BspCNI CTCAG 1 cut(s) 185
BspLI GGNNCC 2 cut(s) 22, 120
BspT107I GGYRCC 2 cut(s) 20, 118
BspTI CTTAAG 1 cut(s) 272
BsrFI RCCGGY 1 cut(s) 185
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 3 cut(s) 16, 24, 219
BssNI GRCGYC 1 cut(s) 119
BssT1I CCWWGG 3 cut(s) 16, 24, 219
Bst4CI ACNGT 1 cut(s) 235
BstACI GRCGYC 1 cut(s) 119
BstAFI CTTAAG 1 cut(s) 272
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 1 cut(s) 172
BstEII GGTNACC 1 cut(s) 188
BstH2I RGCGCY 1 cut(s) 122
BstHHI GCGC 2 cut(s) 9, 121
BstMWI GCNNNNNNNGC 2 cut(s) 29, 125
BstPI GGTNACC 1 cut(s) 188
BsuRI GGCC 1 cut(s) 270
Cac8I GCNNGC 1 cut(s) 34
CfoI GCGC 2 cut(s) 9, 121
Cfr10I RCCGGY 1 cut(s) 185
CspAI ACCGGT 1 cut(s) 185
CviJI RGCY 8 cut(s) 15, 32, 87, 157, 178, 197, 224, 270
CviKI_1 RGCY 8 cut(s) 15, 32, 87, 157, 178, 197, 224, 270
DdeI CTNAG 1 cut(s) 172
DinI GGCGCC 1 cut(s) 120
DriI GACNNNNNGTC 1 cut(s) 237
Eam1105I GACNNNNNGTC 1 cut(s) 237
Ecl136II GAGCTC 1 cut(s) 32
Eco130I CCWWGG 3 cut(s) 16, 24, 219
Eco24I GRGCYC 1 cut(s) 34
Eco53kI GAGCTC 1 cut(s) 32
Eco57I CTGAAG 1 cut(s) 164
Eco91I GGTNACC 1 cut(s) 188
EcoICRI GAGCTC 1 cut(s) 32
EcoO65I GGTNACC 1 cut(s) 188
EcoT14I CCWWGG 3 cut(s) 16, 24, 219
EcoT38I GRGCYC 1 cut(s) 34
EgeI GGCGCC 1 cut(s) 120
EheI GGCGCC 1 cut(s) 120
ErhI CCWWGG 3 cut(s) 16, 24, 219
FaqI GGGAC 2 cut(s) 182, 244
FriOI GRGCYC 1 cut(s) 34
GlaI GCGC 2 cut(s) 8, 120
GsaI CCCAGC 3 cut(s) 105, 197, 201
HaeII RGCGCY 1 cut(s) 122
HaeIII GGCC 1 cut(s) 270
HapII CCGG 1 cut(s) 186
HhaI GCGC 2 cut(s) 9, 121
Hin1I GRCGYC 1 cut(s) 119
Hin6I GCGC 2 cut(s) 7, 119
HinP1I GCGC 2 cut(s) 7, 119
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 2 cut(s) 108, 170
HpaII CCGG 1 cut(s) 186
HphI GGTGA 4 cut(s) 45, 85, 196, 200
Hpy166II GTNNAC 2 cut(s) 53, 204
Hpy188I TCNGA 1 cut(s) 183
Hpy8I GTNNAC 2 cut(s) 53, 204
HpyAV CCTTC 1 cut(s) 130
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4V TGCA 1 cut(s) 128
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 125
HpyF3I CTNAG 1 cut(s) 172
Hsp92I GRCGYC 1 cut(s) 119
HspAI GCGC 2 cut(s) 7, 119
KasI GGCGCC 1 cut(s) 118
LmnI GCTCC 1 cut(s) 29
LpnPI CCDG 7 cut(s) 87, 118, 132, 159, 183, 199, 207
MaeIII GTNAC 3 cut(s) 73, 188, 239
MhlI GDGCHC 2 cut(s) 34, 289
MluCI AATT 1 cut(s) 61
Mly113I GGCGCC 1 cut(s) 119
MlyI GAGTC 1 cut(s) 164
MseI TTAA 1 cut(s) 273
MspA1I CMGCKG 1 cut(s) 197
MspCI CTTAAG 1 cut(s) 272
MspI CCGG 1 cut(s) 186
MwoI GCNNNNNNNGC 2 cut(s) 29, 125
NarI GGCGCC 1 cut(s) 119
NlaIV GGNNCC 2 cut(s) 22, 120
NmuCI GTSAC 3 cut(s) 73, 188, 239
PfeI GAWTC 1 cut(s) 108
PinAI ACCGGT 1 cut(s) 185
PleI GAGTC 1 cut(s) 164
PluTI GGCGCC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 164
Psp124BI GAGCTC 1 cut(s) 34
PspEI GGTNACC 1 cut(s) 188
PspFI CCCAGC 3 cut(s) 101, 193, 197
PspN4I GGNNCC 2 cut(s) 22, 120
PvuII CAGCTG 1 cut(s) 197
SacI GAGCTC 1 cut(s) 34
SaqAI TTAA 1 cut(s) 273
SchI GAGTC 1 cut(s) 164
SduI GDGCHC 2 cut(s) 34, 289
SetI ASST 5 cut(s) 22, 34, 159, 199, 221
SfoI GGCGCC 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 1 cut(s) 61
SspDI GGCGCC 1 cut(s) 118
SstI GAGCTC 1 cut(s) 34
StyI CCWWGG 3 cut(s) 16, 24, 219
TaaI ACNGT 1 cut(s) 235
TasI AATT 1 cut(s) 61
TfiI GAWTC 1 cut(s) 108
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TseFI GTSAC 3 cut(s) 73, 188, 239
Tsp45I GTSAC 3 cut(s) 73, 188, 239
TspDTI ATGAA 1 cut(s) 34
Vha464I CTTAAG 1 cut(s) 272
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.