Rh5BG119400

Photosystem I reaction center subunit psaK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0015469
Physical Location & Seq
Forward (+)
11217272 .. 11218434
1163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG119400.1

Sequence Viewer

Length: 390 bp
ATGGCAGCAGCTACTGTGACTACTTCTCTCCCCCAGTTCAATGGGTTGAGACCCAAGATCTCGTCCAATGTACAGAGCTTGGCTGCTGTTCAACCAATGAGGCGCAAGAGCCAAGGTGCCTTGGGAGCTCGCTGTGATTTCATCGGTTCACCCACCAATTTGATAATGGTGACTACTACAAGCCTAATGTTGTTTGCTGGGAGATTCGGGTTGGCGCCATCTGCAAACAGGAAGGCAACAGCAGGATTGAAGCTTGAAATTAGGGACTCAGGGCTTCAGACCGGTGACCCAGCTGGGTTCACCCTTGCTGATACCTTGGCTTGTGGGACTGTAGGTCACATCATTGGGGTTGGGGTTGTTCTTGGCCTTAAGAACATTGGTGCTCTGTAA

Protein Analysis

129

Amino Acids

13.18

Weight (kDa)

10.52

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSI_PSAK PF01241 52 - 127 1.3e-17 Photosystem I psaG / psaK
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30380
fragaria_vesca FvH4_3g09680
malus_domestica MD00G1109700.v1.1 MD05G1276100.v1.1
prunus_persica Prupe.4G088200_v2.0.a1
pyrus_communis pycom05g25710 pycom10g21120
rosa_chinensis RchiOBHm_Chr5g0015361
rosa_laevigata RLG00000032195
rosa_multiflora Rmu_sc0014430.1_g000001
rosa_rugosa Rorug05G0026800
rosa_samantha Rh5AG120400 Rh5BG119400 Rh5CG129800 Rh5DG118400
rosa_wichuraiana Rw5G010410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 116, 214
AcuI CTGAAG 1 cut(s) 260
AcyI GRCGYC 1 cut(s) 215
AfaI GTAC 1 cut(s) 72
AflII CTTAAG 1 cut(s) 368
AgeI ACCGGT 1 cut(s) 281
AgsI TTSAA 4 cut(s) 40, 92, 250, 257
AhdI GACNNNNNGTC 1 cut(s) 333
AluBI AGCT 5 cut(s) 11, 78, 128, 253, 293
AluI AGCT 5 cut(s) 11, 78, 128, 253, 293
Alw21I GWGCWC 2 cut(s) 130, 385
Alw26I GTCTC 1 cut(s) 43
AlwNI CAGNNNCTG 1 cut(s) 14
AoxI GGCC 1 cut(s) 364
ApeKI GCWGC 3 cut(s) 5, 8, 83
AsiGI ACCGGT 1 cut(s) 281
AspLEI GCGC 2 cut(s) 105, 217
AsuHPI GGTGA 4 cut(s) 141, 181, 292, 296
BanI GGYRCC 2 cut(s) 116, 214
BanII GRGCYC 1 cut(s) 130
Bbv12I GWGCWC 2 cut(s) 130, 385
BbvI GCAGC 3 cut(s) 17, 20, 70
BccI CCATC 1 cut(s) 226
BcoDI GTCTC 1 cut(s) 43
BfmI CTRYAG 1 cut(s) 330
BfoI RGCGCY 1 cut(s) 218
BfrI CTTAAG 1 cut(s) 368
BglII AGATCT 1 cut(s) 57
BisI GCNGC 3 cut(s) 6, 9, 84
BlsI GCNGC 3 cut(s) 7, 10, 85
BmeRI GACNNNNNGTC 1 cut(s) 333
BmiI GGNNCC 2 cut(s) 118, 216
BmrI ACTGGG 1 cut(s) 28
BmuI ACTGGG 1 cut(s) 28
BsaHI GRCGYC 1 cut(s) 215
BsaI GGTCTC 1 cut(s) 43
BsaJI CCNNGG 3 cut(s) 112, 120, 315
BsaWI WCCGGW 1 cut(s) 281
Bse118I RCCGGY 1 cut(s) 281
Bse1I ACTGG 1 cut(s) 34
BseDI CCNNGG 3 cut(s) 112, 120, 315
BseMII CTCAG 1 cut(s) 282
BseNI ACTGG 1 cut(s) 34
BseXI GCAGC 3 cut(s) 17, 20, 70
BseYI CCCAGC 3 cut(s) 197, 289, 293
BshFI GGCC 1 cut(s) 366
BshNI GGYRCC 2 cut(s) 116, 214
BshTI ACCGGT 1 cut(s) 281
BsiHKAI GWGCWC 2 cut(s) 130, 385
BsiSI CCGG 1 cut(s) 282
BslFI GGGAC 2 cut(s) 278, 340
BsmAI GTCTC 1 cut(s) 43
BsmFI GGGAC 2 cut(s) 278, 340
BsnI GGCC 1 cut(s) 366
Bso31I GGTCTC 1 cut(s) 43
Bsp1286I GDGCHC 2 cut(s) 130, 385
Bsp1407I TGTACA 1 cut(s) 70
Bsp143I GATC 1 cut(s) 57
BspANI GGCC 1 cut(s) 366
BspCNI CTCAG 1 cut(s) 281
BspLI GGNNCC 2 cut(s) 118, 216
BspT107I GGYRCC 2 cut(s) 116, 214
BspTI CTTAAG 1 cut(s) 368
BspTNI GGTCTC 1 cut(s) 43
BsrFI RCCGGY 1 cut(s) 281
BsrGI TGTACA 1 cut(s) 70
BsrI ACTGG 1 cut(s) 34
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 3 cut(s) 112, 120, 315
BssMI GATC 1 cut(s) 57
BssNI GRCGYC 1 cut(s) 215
BssT1I CCWWGG 3 cut(s) 112, 120, 315
Bst4CI ACNGT 2 cut(s) 16, 331
BstACI GRCGYC 1 cut(s) 215
BstAFI CTTAAG 1 cut(s) 368
BstAUI TGTACA 1 cut(s) 70
BstC8I GCNNGC 1 cut(s) 130
BstDEI CTNAG 1 cut(s) 268
BstEII GGTNACC 1 cut(s) 284
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 2 cut(s) 105, 217
BstKTI GATC 1 cut(s) 60
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 1 cut(s) 57
BstMWI GCNNNNNNNGC 2 cut(s) 125, 221
BstPI GGTNACC 1 cut(s) 284
BstSFI CTRYAG 1 cut(s) 330
BstV1I GCAGC 3 cut(s) 17, 20, 70
BstX2I RGATCY 1 cut(s) 57
BstXI CCANNNNNNTGG 1 cut(s) 41
BstYI RGATCY 1 cut(s) 57
BsuRI GGCC 1 cut(s) 366
Cac8I GCNNGC 1 cut(s) 130
CaiI CAGNNNCTG 1 cut(s) 14
CfoI GCGC 2 cut(s) 105, 217
Cfr10I RCCGGY 1 cut(s) 281
Csp6I GTAC 1 cut(s) 71
CspAI ACCGGT 1 cut(s) 281
CviQI GTAC 1 cut(s) 71
DdeI CTNAG 1 cut(s) 268
DinI GGCGCC 1 cut(s) 216
DpnI GATC 1 cut(s) 59
DpnII GATC 1 cut(s) 57
DriI GACNNNNNGTC 1 cut(s) 333
Eam1105I GACNNNNNGTC 1 cut(s) 333
Ecl136II GAGCTC 1 cut(s) 128
Eco130I CCWWGG 3 cut(s) 112, 120, 315
Eco24I GRGCYC 1 cut(s) 130
Eco31I GGTCTC 1 cut(s) 43
Eco53kI GAGCTC 1 cut(s) 128
Eco57I CTGAAG 1 cut(s) 260
Eco91I GGTNACC 1 cut(s) 284
EcoICRI GAGCTC 1 cut(s) 128
EcoO65I GGTNACC 1 cut(s) 284
EcoT14I CCWWGG 3 cut(s) 112, 120, 315
EcoT38I GRGCYC 1 cut(s) 130
EgeI GGCGCC 1 cut(s) 216
EheI GGCGCC 1 cut(s) 216
ErhI CCWWGG 3 cut(s) 112, 120, 315
FaqI GGGAC 2 cut(s) 278, 340
Fnu4HI GCNGC 3 cut(s) 6, 9, 84
FriOI GRGCYC 1 cut(s) 130
Fsp4HI GCNGC 3 cut(s) 6, 9, 84
GlaI GCGC 2 cut(s) 104, 216
GluI GCNGC 3 cut(s) 6, 9, 84
GsaI CCCAGC 3 cut(s) 201, 293, 297
HaeII RGCGCY 1 cut(s) 218
HaeIII GGCC 1 cut(s) 366
HapII CCGG 1 cut(s) 282
HhaI GCGC 2 cut(s) 105, 217
Hin1I GRCGYC 1 cut(s) 215
Hin6I GCGC 2 cut(s) 103, 215
HinP1I GCGC 2 cut(s) 103, 215
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 2 cut(s) 204, 266
HpaII CCGG 1 cut(s) 282
HphI GGTGA 4 cut(s) 141, 181, 292, 296
Hpy166II GTNNAC 2 cut(s) 149, 300
Hpy188I TCNGA 1 cut(s) 279
Hpy8I GTNNAC 2 cut(s) 149, 300
HpyAV CCTTC 1 cut(s) 226
HpyCH4III ACNGT 2 cut(s) 16, 331
HpyCH4V TGCA 1 cut(s) 224
HpyF10VI GCNNNNNNNGC 2 cut(s) 125, 221
HpyF3I CTNAG 1 cut(s) 268
Hsp92I GRCGYC 1 cut(s) 215
HspAI GCGC 2 cut(s) 103, 215
KasI GGCGCC 1 cut(s) 214
Kzo9I GATC 1 cut(s) 57
LmnI GCTCC 1 cut(s) 125
LpnPI CCDG 8 cut(s) 47, 183, 214, 228, 255, 279, 295, 303
Lsp1109I GCAGC 3 cut(s) 17, 20, 70
MaeIII GTNAC 4 cut(s) 16, 169, 284, 335
MalI GATC 1 cut(s) 59
MboI GATC 1 cut(s) 57
MflI RGATCY 1 cut(s) 57
MhlI GDGCHC 2 cut(s) 130, 385
MluCI AATT 2 cut(s) 157, 258
Mly113I GGCGCC 1 cut(s) 215
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 1 cut(s) 93
MseI TTAA 1 cut(s) 369
MspA1I CMGCKG 1 cut(s) 293
MspCI CTTAAG 1 cut(s) 368
MspI CCGG 1 cut(s) 282
MwoI GCNNNNNNNGC 2 cut(s) 125, 221
NarI GGCGCC 1 cut(s) 215
NdeII GATC 1 cut(s) 57
NlaIV GGNNCC 2 cut(s) 118, 216
NmuCI GTSAC 4 cut(s) 16, 169, 284, 335
PfeI GAWTC 1 cut(s) 204
PinAI ACCGGT 1 cut(s) 281
PkrI GCNGC 3 cut(s) 7, 10, 85
PleI GAGTC 1 cut(s) 260
PluTI GGCGCC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 260
Psp124BI GAGCTC 1 cut(s) 130
PspEI GGTNACC 1 cut(s) 284
PspFI CCCAGC 3 cut(s) 197, 289, 293
PspN4I GGNNCC 2 cut(s) 118, 216
PstNI CAGNNNCTG 1 cut(s) 14
PsuI RGATCY 1 cut(s) 57
PvuII CAGCTG 1 cut(s) 293
RsaI GTAC 1 cut(s) 72
RsaNI GTAC 1 cut(s) 71
SacI GAGCTC 1 cut(s) 130
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 3 cut(s) 6, 9, 84
Sau3AI GATC 1 cut(s) 57
SchI GAGTC 1 cut(s) 260
SduI GDGCHC 2 cut(s) 130, 385
SetI ASST 8 cut(s) 13, 80, 118, 130, 255, 295, 317, 337
SfcI CTRYAG 1 cut(s) 330
SfoI GGCGCC 1 cut(s) 216
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 2 cut(s) 157, 258
SspDI GGCGCC 1 cut(s) 214
SstI GAGCTC 1 cut(s) 130
StyI CCWWGG 3 cut(s) 112, 120, 315
TaaI ACNGT 2 cut(s) 16, 331
TasI AATT 2 cut(s) 157, 258
TatI WGTACW 1 cut(s) 70
TfiI GAWTC 1 cut(s) 204
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TseFI GTSAC 4 cut(s) 16, 169, 284, 335
TseI GCWGC 3 cut(s) 5, 8, 83
Tsp45I GTSAC 4 cut(s) 16, 169, 284, 335
TspDTI ATGAA 1 cut(s) 130
Vha464I CTTAAG 1 cut(s) 368
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.