Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005331

Physical Location & Seq

Genomic Coordinates Reverse (-)

11158618 .. 11163274

Gene Structure 4657 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G31230.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2736 bp

ATGCCGGTGGTTTCTCTGGCTAAGGTTGTTACTTCTCCGGCGGTGGCCGGAGATTTAGCGGTTCGTGTTCCGTTCATTTATGGGAAACGACTAGTGTCGAATCGTGTTTCTTTCGGGAAATTGAGGCGCCGGAGTTGTATAGGTCAATGCGTAAGAAGCGAATTGCAAAGTCCTCGTGTCTTAGGTTCCGTCACAGATTTAGCGTTGGATAATTCTGTGGAGAATGGTCATCTTCCCAAAGGAGATTCATGGGCTGTACACAAATTTGGAGGAACTTGTGTGGGAAATTCTGAGAGGATAAAGGATGTTGCTGCTGTTGTTGTTAAGGATGACTCTGAGAGGAAGTTGGTGGTTGTGTCAGCAATGTCGAAAGTCACTGATATGATGTATGATCTCATTCACAGAGCAGAGTCTCGTGATGATTCATATCTGTCTGCTTTGAGTGGTGTTCTTGAAAAGCACCGAGCAACTGCTGTTGACTTGCTTGATGGAGATGAACTCTCAAGTTTCTTGGCTCGGTTAAATGATGATATAAATAATCTCAAAGCAATGCTTCGTGCCATTTACATAGCTGGTCATGCAACCGAATCTTTCTCAGACTTCGTTGTTGGCCACGGAGAGTTGTGGTCTGCTCAGATGTTAGCTGCTGTTGTGAGAAAGAGCGGATTGGACTGCACTTGGATGGATGCAAGGGATGTGCTTGTTGTTATTCCAACGAGCTCTAATCAAGTTGACCCTGACTTTGTGGAATCAGAAAAAAGACTGGAAAAATGGTTTACTCAGAACTCGGCAAAGATTATTATAGCAACTGGTTTCATAGCCAGTACACCACAGAACATTCCAACGACTCTTAAAAGGGATGGGAGTGACTTCTCTGCAGCTATAATGAGTGCTCTGTTTAGATCTCACCAACTCACAATCTGGACAGATGTTGATGGTGTGTACAGTGCAGATCCCAGGAAAGTTAGTGAAGCTGTTGTGCTGAAGACTCTTTCTTATCAAGAGGCTTGGGAAATGTCTTACTTTGGGGCAAACGTTTTACATCCTCGGACCATTATTCCAGTGATGAAATATGACATTCCAATTGTAATAAGGAATATTTTCAACCTCTCTGCCCCTGGAACAATGATATGCCGGCAGATTGATGATGAAGATGGATTCAAATTAGACGCTCCTGTGAAAGGATTTGCGACGATTGACAATTTGGCTCTTGTCAATGTAGAAGGAACTGGAATGGCTGGTGTTCCTGGTACTGCCAGTGCCATTTTTTCTGCTGTCAAGGAAGTTGGAGCCAATGTGATTATGATATCGCAGGCTAGTAGCGAGCATTCTGTGTGCTTTGCTGTACCTGAGAAGGAAGTGAAAGCTGTTTCTGAAGCATTGAACTCAAGATTTCGTCAAGCTTTGGCTGGTGGCCGCCTTTCCCAGATTGAAATCATCCCTAATTGTAGCATATTAGCAGCAGTTGGCCAGAAAATGGCGAGCACTCCTGGTGTTTCTGCCACTTTTTTTAATGCATTAGCAAAGGCCAATATCAACATCCGTGCTATAGCCCAAGGTTGCTCCGAGTTCAATATTACAGTAGTCGTCAAGCGTGAAGACTGCATCAGGGCATTAAGAGCTGTGCACTCAAGATTTTACCTTTCGAGAACCACTTTGGCAGTGGGAATCATAGGACCGGGATTAATTGGTGGAACCTTACTTGATCAGATTAGAGATCAGGCGGCAGTGCTCAAAGAAGAATTTAAAATTGACTTGCGTGTTATAGGGATCACGGGCTCAAGTAAAATGTTGATGAGTGAATCGGGGATTGACTTATCAAGATGGAGAGAGCTTATGAAAGAAGAGGGAGAAAAAGCTGACATGGAGAAGTTCACCCAATATGTGAAGGGAAATCATTTCATCCCAAACTCTGTTATGGTTGATTGTACAGCCGATGCTGACATCGCTAGCTGTTACTACGACTGGTTGCTACGAGGAATTCATGTGGTCACTCCGAACAAAAAGGCTAACTCTGGACCACTTGATCAGTATCTAAAGATCAGAGATCTTCAACGAAAATCGTACACACATTACTTTTATGAAGCCACCGTTGGAGCTGGTCTTCCAATTATTAGCACCTTACGTGGTCTCCTCGAAACAGGGGATAAAATACTGCGAATTGAGGGAATTTTCAGTGGTACTTTAAGTTACCTCTTCAACAACTTTGTCGGCACCAGAAGCTTCAGTGAAGTTGTAGCAGAAGCAAAGCAAGCAGGTTTCACAGAACCAGATCCACGAGATGATCTATCTGGAACAGATGTTGCCAGAAAAGTAACAATCCTTGCCAGAGAATCAGGCTTAAAATTGGATCTTGAGGGCCTTCCAGTCCAGAATCTTGTGCCAAAGCCGTTACAAGCTTGTGCATCAGCAGAAGAGTTCATGGAGAAGCTTCCTCAGTTTGATGAAGAATTATCCAAACAAAGAGAAGAGGCTGAAGCAGCAGGAGAAGTCTTGAGATACGTAGGAGTTGTAGATGCAGTAGAGAAAAAGGGAACAGTCGAGTTGAAACGGTACAAAAAAGATCATCCGTTTGCTCAGCTATCGGGTGCTGATAACATCATCGCTTTCACAACCAAACGGTACAAAGAACAGCCTCTGATTGTTCGTGGACCTGGTGCTGGTGCTCAAGTCACAGCCGGTGGAATCTTCAGTGACATTCTTCGCCTTGCTTTCTATCTCGGGGCTCCGTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004072 GO:0004412 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009069 GO:0009070 GO:0009090 GO:0009092 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016301 GO:0016310 GO:0016491 GO:0016614 GO:0016616 GO:0016740 GO:0016772 GO:0016774 GO:0019202 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0055114 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00260 ko00261 ko00270 ko00300 ko01100 ko01110 ko01120 ko01130 ko01230 map00260 map00261 map00270 map00300 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

AA_kinase ACT ACT_7 Homoserine_dh NAD_binding_3

Protein Analysis

911

Amino Acids

99.4

Weight (kDa)

6.34

Isoelectric Point (pI)

38.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_kinase	PF00696	84 - 366	4.5e-42	Amino acid kinase family
ACT_7	PF13840	400 - 462	8.9e-06	ACT domain
ACT_9	PF22468	405 - 465	6.4e-18	ACT domain
ACT_7	PF13840	479 - 544	1.9e-10	ACT domain
ACT_9	PF22468	487 - 546	1.8e-22	ACT domain
ACT	PF01842	493 - 534	3.5e-06	ACT domain
NAD_binding_3	PF03447	559 - 695	3.4e-18	Homoserine dehydrogenase, NAD binding domain
Homoserine_dh	PF00742	703 - 901	5.4e-51	Homoserine dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005331)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31230 AT4G19710 AT4G19710
fragaria_vesca	FvH4_2g00800 FvH4_2g00800 FvH4_2g00800 FvH4_2g00800
malus_domestica	MD05G1003800.v1.1 MD10G1004700.v1.1
prunus_persica	Prupe.5G007500_v2.0.a1 Prupe.5G007500_v2.0.a1 Prupe.5G007500_v2.0.a1 Prupe.8G007000_v2.0.a1 Prupe.8G007000_v2.0.a1
pyrus_communis	pycom05g00330 pycom10g00370 pycom10g00380
rosa_chinensis	RchiOBHm_Chr6g0251231
rosa_laevigata	RLG00000015472
rosa_multiflora	Rmu_sc0001637.1_g000003 Rmu_sc0015396.1_g000003
rosa_roxburghii	Rroxscaffold_7G00216900
rosa_rugosa	Rorug05G0500900.1 Rorug05G0501000
rosa_samantha	Rh6BG001700 Rh6CG007400 Rh6DG007800
rosa_wichuraiana	Rw6G001010 Rw6G001120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2246
AccB1I	GGYRCC	2 cut(s)	126, 2213
AccB7I	CCANNNNNTGG	1 cut(s)	1477
AccBSI	CCGCTC	1 cut(s)	663
AciI	CCGC	5 cut(s)	41, 59, 663, 1417, 1724
AclI	AACGTT	1 cut(s)	1035
AclWI	GGATC	4 cut(s)	947, 1778, 2267, 2358
AcoI	YGGCCR	4 cut(s)	45, 610, 1414, 1468
AcsI	RAATTY	5 cut(s)	263, 286, 1742, 1980, 2169
AcuI	CTGAAG	5 cut(s)	1004, 1395, 2209, 2496, 2674
AcyI	GRCGYC	1 cut(s)	127
AfiI	CCNNNNNNNGG	3 cut(s)	1181, 1477, 2660
AgsI	TTSAA	9 cut(s)	455, 1105, 1162, 1384, 1433, 1573, 2054, 2200, 2548
AhlI	ACTAGT	1 cut(s)	91
AjnI	CCWGG	5 cut(s)	956, 1117, 1246, 1489, 2653
AjuI	GAANNNNNNNTTGG	2 cut(s)	2076, 2108
Alw21I	GWGCWC	6 cut(s)	722, 895, 1487, 1629, 1734, 2668
Alw26I	GTCTC	2 cut(s)	417, 2135
Alw44I	GTGCAC	1 cut(s)	1625
AlwI	GGATC	4 cut(s)	947, 1778, 2267, 2358
AlwNI	CAGNNNCTG	1 cut(s)	2638
Ama87I	CYCGRG	1 cut(s)	2720
AoxI	GGCC	6 cut(s)	45, 610, 1414, 1468, 1527, 2359
ApaLI	GTGCAC	1 cut(s)	1625
ApeKI	GCWGC	5 cut(s)	311, 644, 878, 1460, 2480
ApoI	RAATTY	5 cut(s)	263, 286, 1742, 1980, 2169
ArsI	GACNNNNNNTTYG	2 cut(s)	725, 757
AseI	ATTAAT	1 cut(s)	1685
Asp700I	GAANNNNTTC	1 cut(s)	1100
AspLEI	GCGC	1 cut(s)	129
AspS9I	GGNCC	5 cut(s)	1050, 1676, 2018, 2359, 2651
AsuC2I	CCSGG	1 cut(s)	1680
AsuHPI	GGTGA	2 cut(s)	899, 1867
AsuNHI	GCTAGC	1 cut(s)	1949
AvaI	CYCGRG	1 cut(s)	2720
AvaII	GGWCC	4 cut(s)	1050, 1676, 2018, 2651
BaeGI	GKGCMC	1 cut(s)	1629
BalI	TGGCCA	2 cut(s)	612, 1470
BanI	GGYRCC	2 cut(s)	126, 2213
BanII	GRGCYC	3 cut(s)	722, 1781, 2728
BauI	CACGAG	3 cut(s)	174, 414, 2277
BbsI	GAAGAC	3 cut(s)	992, 1605, 2096
Bbv12I	GWGCWC	6 cut(s)	722, 895, 1487, 1629, 1734, 2668
BbvI	GCAGC	5 cut(s)	298, 631, 890, 1472, 2492
BccI	CCATC	6 cut(s)	482, 676, 854, 929, 1148, 1818
BceAI	ACGGC	1 cut(s)	2374
BcgI	CGANNNNNNTGC	2 cut(s)	155, 189
BciT130I	CCWGG	5 cut(s)	958, 1119, 1248, 1491, 2655
BclI	TGATCA	2 cut(s)	1705, 2026
BcnI	CCSGG	1 cut(s)	1680
BcoDI	GTCTC	2 cut(s)	417, 2135
BcuI	ACTAGT	1 cut(s)	91
BfaI	CTAG	3 cut(s)	92, 1317, 1950
BfmI	CTRYAG	2 cut(s)	876, 1548
BfoI	RGCGCY	1 cut(s)	130
BfuAI	ACCTGC	1 cut(s)	2246
BglII	AGATCT	2 cut(s)	902, 2047
BisI	GCNGC	7 cut(s)	312, 645, 879, 1417, 1461, 1725, 2481
BlpI	GCTNAGC	1 cut(s)	2577
BlsI	GCNGC	7 cut(s)	313, 646, 880, 1418, 1462, 1726, 2482
Bme1390I	CCNGG	6 cut(s)	958, 1119, 1248, 1491, 1680, 2655
Bme18I	GGWCC	4 cut(s)	1050, 1676, 2018, 2651
BmeT110I	CYCGRG	1 cut(s)	2720
BmgT120I	GGNCC	5 cut(s)	1050, 1676, 2018, 2359, 2651
BmiI	GGNNCC	6 cut(s)	128, 187, 1291, 1696, 2215, 2727
BmrFI	CCNGG	6 cut(s)	958, 1119, 1248, 1491, 1680, 2655
BmsI	GCATC	5 cut(s)	676, 1614, 1927, 2414, 2506
BmtI	GCTAGC	1 cut(s)	1953
BoxI	GACNNNNGTC	1 cut(s)	94
BpiI	GAAGAC	3 cut(s)	992, 1605, 2096
BplI	GAGNNNNNCTC	2 cut(s)	483, 515
Bpu10I	CCTNAGC	1 cut(s)	21
Bpu1102I	GCTNAGC	1 cut(s)	2577
BpuEI	CTTGAG	7 cut(s)	487, 1372, 1615, 1765, 2375, 2515, 2652
BpuMI	CCSGG	1 cut(s)	1680
BsaAI	YACGTR	2 cut(s)	2126, 2503
BsaBI	GATNNNNATC	3 cut(s)	426, 1433, 2031
BsaHI	GRCGYC	1 cut(s)	127
BsaI	GGTCTC	1 cut(s)	2135
BsaJI	CCNNGG	5 cut(s)	613, 956, 1046, 1117, 1555
BsaXI	ACNNNNNCTCC	4 cut(s)	1281, 1311, 2115, 2145
Bsc4I	CCNNNNNNNGG	3 cut(s)	1181, 1477, 2660
Bse118I	RCCGGY	3 cut(s)	4, 1134, 2678
Bse1I	ACTGG	8 cut(s)	768, 814, 822, 1061, 1234, 1257, 1970, 2366
Bse3DI	GCAATG	2 cut(s)	369, 555
Bse8I	GATNNNNATC	3 cut(s)	426, 1433, 2031
BseBI	CCWGG	5 cut(s)	958, 1119, 1248, 1491, 2655
BseDI	CCNNGG	5 cut(s)	613, 956, 1046, 1117, 1555
BseJI	GATNNNNATC	3 cut(s)	426, 1433, 2031
BseLI	CCNNNNNNNGG	3 cut(s)	1181, 1477, 2660
BseMI	GCAATG	2 cut(s)	369, 555
BseMII	CTCAG	8 cut(s)	282, 327, 609, 647, 794, 1341, 2450, 2591
BseNI	ACTGG	8 cut(s)	768, 814, 822, 1061, 1234, 1257, 1970, 2366
BseRI	GAGGAG	1 cut(s)	2123
BseSI	GKGCMC	1 cut(s)	1629
BseXI	GCAGC	5 cut(s)	298, 631, 890, 1472, 2492
BsgI	GTGCAG	2 cut(s)	658, 969
BshFI	GGCC	6 cut(s)	47, 612, 1416, 1470, 1529, 2361
BshNI	GGYRCC	2 cut(s)	126, 2213
BsiHKAI	GWGCWC	6 cut(s)	722, 895, 1487, 1629, 1734, 2668
BsiHKCI	CYCGRG	1 cut(s)	2720
BsiSI	CCGG	7 cut(s)	5, 38, 48, 130, 1135, 1679, 2679
BslI	CCNNNNNNNGG	3 cut(s)	1181, 1477, 2660
BsmAI	GTCTC	2 cut(s)	417, 2135
BsmI	GAATGC	1 cut(s)	1327
BsnI	GGCC	6 cut(s)	47, 612, 1416, 1470, 1529, 2361
Bso31I	GGTCTC	1 cut(s)	2135
BsoBI	CYCGRG	1 cut(s)	2720
Bsp1286I	GDGCHC	8 cut(s)	722, 895, 1487, 1629, 1734, 1781, 2668, 2728
Bsp1407I	TGTACA	3 cut(s)	256, 942, 1928
Bsp1720I	GCTNAGC	1 cut(s)	2577
BspACI	CCGC	5 cut(s)	41, 59, 663, 1417, 1724
BspANI	GGCC	6 cut(s)	47, 612, 1416, 1470, 1529, 2361
BspCNI	CTCAG	8 cut(s)	283, 328, 608, 646, 793, 1342, 2449, 2590
BspLI	GGNNCC	6 cut(s)	128, 187, 1291, 1696, 2215, 2727
BspMAI	CTGCAG	1 cut(s)	880
BspMI	ACCTGC	1 cut(s)	2246
BspOI	GCTAGC	1 cut(s)	1953
BspPI	GGATC	4 cut(s)	947, 1778, 2267, 2358
BspT107I	GGYRCC	2 cut(s)	126, 2213
BspTNI	GGTCTC	1 cut(s)	2135
BsrBI	CCGCTC	1 cut(s)	663
BsrDI	GCAATG	2 cut(s)	369, 555
BsrFI	RCCGGY	3 cut(s)	4, 1134, 2678
BsrGI	TGTACA	3 cut(s)	256, 942, 1928
BsrI	ACTGG	8 cut(s)	768, 814, 822, 1061, 1234, 1257, 1970, 2366
BssAI	RCCGGY	3 cut(s)	4, 1134, 2678
BssECI	CCNNGG	5 cut(s)	613, 956, 1046, 1117, 1555
BssNI	GRCGYC	1 cut(s)	127
BssSI	CACGAG	3 cut(s)	174, 414, 2277
BssT1I	CCWWGG	1 cut(s)	1555
Bst2BI	CACGAG	3 cut(s)	174, 414, 2277
Bst2UI	CCWGG	5 cut(s)	958, 1119, 1248, 1491, 2655
Bst4CI	ACNGT	6 cut(s)	947, 1582, 2092, 2539, 2553, 2622
Bst6I	CTCTTC	4 cut(s)	1839, 2201, 2409, 2463
BstACI	GRCGYC	1 cut(s)	127
BstAUI	TGTACA	3 cut(s)	256, 942, 1928
BstBAI	YACGTR	2 cut(s)	2126, 2503
BstC8I	GCNNGC	6 cut(s)	1136, 1314, 1325, 1483, 1951, 2253
BstDSI	CCRYGG	1 cut(s)	613
BstENI	CCTNNNNNAGG	1 cut(s)	1179
BstH2I	RGCGCY	1 cut(s)	130
BstHHI	GCGC	1 cut(s)	129
BstMAI	GTCTC	2 cut(s)	417, 2135
BstMWI	GCNNNNNNNGC	7 cut(s)	156, 578, 1619, 1946, 2220, 2252, 2480
BstNI	CCWGG	5 cut(s)	958, 1119, 1248, 1491, 2655
BstPAI	GACNNNNGTC	1 cut(s)	94
BstSCI	CCNGG	6 cut(s)	956, 1117, 1246, 1489, 1678, 2653
BstSFI	CTRYAG	2 cut(s)	876, 1548
BstSLI	GKGCMC	1 cut(s)	1629
BstSNI	TACGTA	1 cut(s)	2503
BstV1I	GCAGC	5 cut(s)	298, 631, 890, 1472, 2492
BstV2I	GAAGAC	3 cut(s)	992, 1605, 2096
BstX2I	RGATCY	5 cut(s)	902, 952, 2047, 2272, 2350
BstYI	RGATCY	5 cut(s)	902, 952, 2047, 2272, 2350
BsuRI	GGCC	6 cut(s)	47, 612, 1416, 1470, 1529, 2361
BtgI	CCRYGG	1 cut(s)	613
BtgZI	GCGATG	2 cut(s)	1930, 2587
BtsI	GCAGTG	2 cut(s)	1668, 1734
BtsIMutI	CAGTG	9 cut(s)	375, 952, 1068, 1264, 1668, 1734, 2182, 2233, 2698
BveI	ACCTGC	1 cut(s)	2246
Cac8I	GCNNGC	6 cut(s)	1136, 1314, 1325, 1483, 1951, 2253
CaiI	CAGNNNCTG	1 cut(s)	2638
CfoI	GCGC	1 cut(s)	129
Cfr10I	RCCGGY	3 cut(s)	4, 1134, 2678
Cfr13I	GGNCC	5 cut(s)	1050, 1676, 2018, 2359, 2651
CseI	GACGC	1 cut(s)	1178
CsiI	ACCWGGT	1 cut(s)	2653
CviAII	CATG	5 cut(s)	249, 578, 1864, 1985, 2422
DinI	GGCGCC	1 cut(s)	128
DraI	TTTAAA	1 cut(s)	1747
EaeI	YGGCCR	4 cut(s)	45, 610, 1414, 1468
Eam1104I	CTCTTC	4 cut(s)	1839, 2201, 2409, 2463
EarI	CTCTTC	4 cut(s)	1839, 2201, 2409, 2463
Ecl136II	GAGCTC	1 cut(s)	720
Eco105I	TACGTA	1 cut(s)	2503
Eco130I	CCWWGG	1 cut(s)	1555
Eco24I	GRGCYC	3 cut(s)	722, 1781, 2728
Eco31I	GGTCTC	1 cut(s)	2135
Eco32I	GATATC	1 cut(s)	1308
Eco47I	GGWCC	4 cut(s)	1050, 1676, 2018, 2651
Eco53kI	GAGCTC	1 cut(s)	720
Eco57I	CTGAAG	5 cut(s)	1004, 1395, 2209, 2496, 2674
Eco88I	CYCGRG	1 cut(s)	2720
EcoICRI	GAGCTC	1 cut(s)	720
EcoNI	CCTNNNNNAGG	1 cut(s)	1179
EcoO109I	RGGNCCY	1 cut(s)	2359
EcoRI	GAATTC	1 cut(s)	1980
EcoRII	CCWGG	5 cut(s)	956, 1117, 1246, 1489, 2653
EcoRV	GATATC	1 cut(s)	1308
EcoT14I	CCWWGG	1 cut(s)	1555
EcoT22I	ATGCAT	1 cut(s)	1519
EcoT38I	GRGCYC	3 cut(s)	722, 1781, 2728
EgeI	GGCGCC	1 cut(s)	128
EheI	GGCGCC	1 cut(s)	128
ErhI	CCWWGG	1 cut(s)	1555
FaeI	CATG	5 cut(s)	252, 581, 1867, 1988, 2425
FalI	AAGNNNNNCTT	2 cut(s)	537, 569
FatI	CATG	5 cut(s)	248, 577, 1863, 1984, 2421
FbaI	TGATCA	2 cut(s)	1705, 2026
Fnu4HI	GCNGC	7 cut(s)	312, 645, 879, 1417, 1461, 1725, 2481
FriOI	GRGCYC	3 cut(s)	722, 1781, 2728
Fsp4HI	GCNGC	7 cut(s)	312, 645, 879, 1417, 1461, 1725, 2481
FspBI	CTAG	3 cut(s)	92, 1317, 1950
GlaI	GCGC	1 cut(s)	128
GluI	GCNGC	7 cut(s)	312, 645, 879, 1417, 1461, 1725, 2481
HaeII	RGCGCY	1 cut(s)	130
HaeIII	GGCC	6 cut(s)	47, 612, 1416, 1470, 1529, 2361
HapII	CCGG	7 cut(s)	5, 38, 48, 130, 1135, 1679, 2679
HgaI	GACGC	1 cut(s)	1178
HhaI	GCGC	1 cut(s)	129
Hin1I	GRCGYC	1 cut(s)	127
Hin1II	CATG	5 cut(s)	252, 581, 1867, 1988, 2425
Hin6I	GCGC	1 cut(s)	127
HinP1I	GCGC	1 cut(s)	127
HincII	GTYRAC	2 cut(s)	478, 733
HindII	GTYRAC	2 cut(s)	478, 733
HindIII	AAGCTT	4 cut(s)	1401, 2221, 2397, 2429
HpaII	CCGG	7 cut(s)	5, 38, 48, 130, 1135, 1679, 2679
HphI	GGTGA	2 cut(s)	899, 1867
Hpy99I	CGWCG	1 cut(s)	1195
HpyAV	CCTTC	4 cut(s)	1217, 1348, 1882, 2372
HpyCH4III	ACNGT	6 cut(s)	947, 1582, 2092, 2539, 2553, 2622
HpyCH4IV	ACGT	3 cut(s)	1035, 2125, 2502
HpyF10VI	GCNNNNNNNGC	7 cut(s)	156, 578, 1619, 1946, 2220, 2252, 2480
HpySE526I	ACGT	3 cut(s)	1035, 2125, 2502
Hsp92I	GRCGYC	1 cut(s)	127
Hsp92II	CATG	5 cut(s)	252, 581, 1867, 1988, 2425
HspAI	GCGC	1 cut(s)	127
KasI	GGCGCC	1 cut(s)	126
KroI	GCCGGC	1 cut(s)	1134
KroNI	GCCGGC	1 cut(s)	1136
Ksp22I	TGATCA	2 cut(s)	1705, 2026
LmnI	GCTCC	5 cut(s)	1177, 1289, 1568, 2096, 2731
Lsp1109I	GCAGC	5 cut(s)	298, 631, 890, 1472, 2492
LweI	GCATC	5 cut(s)	676, 1614, 1927, 2414, 2506
MabI	ACCWGGT	1 cut(s)	2653
MaeI	CTAG	3 cut(s)	92, 1317, 1950
MaeII	ACGT	3 cut(s)	1035, 2125, 2502
MbiI	CCGCTC	1 cut(s)	663
MfeI	CAATTG	1 cut(s)	1083
MflI	RGATCY	5 cut(s)	902, 952, 2047, 2272, 2350
MhlI	GDGCHC	8 cut(s)	722, 895, 1487, 1629, 1734, 1781, 2668, 2728
MlsI	TGGCCA	2 cut(s)	612, 1470
MluNI	TGGCCA	2 cut(s)	612, 1470
Mly113I	GGCGCC	1 cut(s)	127
MlyI	GAGTC	4 cut(s)	326, 419, 841, 982
MmeI	TCCRAC	5 cut(s)	186, 737, 866, 1267, 2074
Mox20I	TGGCCA	2 cut(s)	612, 1470
Mph1103I	ATGCAT	1 cut(s)	1519
MroNI	GCCGGC	1 cut(s)	1134
MroXI	GAANNNNTTC	1 cut(s)	1100
MscI	TGGCCA	2 cut(s)	612, 1470
MslI	CAYNNNNRTG	2 cut(s)	380, 680
Msp20I	TGGCCA	2 cut(s)	612, 1470
MspI	CCGG	7 cut(s)	5, 38, 48, 130, 1135, 1679, 2679
MspR9I	CCNGG	6 cut(s)	958, 1119, 1248, 1491, 1680, 2655
MunI	CAATTG	1 cut(s)	1083
Mva1269I	GAATGC	1 cut(s)	1327
MvaI	CCWGG	5 cut(s)	958, 1119, 1248, 1491, 2655
MwoI	GCNNNNNNNGC	7 cut(s)	156, 578, 1619, 1946, 2220, 2252, 2480
NaeI	GCCGGC	1 cut(s)	1136
NarI	GGCGCC	1 cut(s)	127
NciI	CCSGG	1 cut(s)	1680
NgoMIV	GCCGGC	1 cut(s)	1134
NheI	GCTAGC	1 cut(s)	1949
NlaIII	CATG	5 cut(s)	252, 581, 1867, 1988, 2425
NlaIV	GGNNCC	6 cut(s)	128, 187, 1291, 1696, 2215, 2727
NmeAIII	GCCGAG	1 cut(s)	767
NmuCI	GTSAC	6 cut(s)	190, 373, 866, 1990, 2671, 2693
NsiI	ATGCAT	1 cut(s)	1519
PctI	GAATGC	1 cut(s)	1327
PdiI	GCCGGC	1 cut(s)	1136
PdmI	GAANNNNTTC	1 cut(s)	1100
PflMI	CCANNNNNTGG	1 cut(s)	1477
PkrI	GCNGC	7 cut(s)	313, 646, 880, 1418, 1462, 1726, 2482
PleI	GAGTC	4 cut(s)	326, 418, 841, 982
PluTI	GGCGCC	1 cut(s)	130
PpsI	GAGTC	4 cut(s)	326, 418, 841, 982
Ppu21I	YACGTR	2 cut(s)	2126, 2503
PshAI	GACNNNNGTC	1 cut(s)	94
PshBI	ATTAAT	1 cut(s)	1685
Psp124BI	GAGCTC	1 cut(s)	722
Psp1406I	AACGTT	1 cut(s)	1035
Psp6I	CCWGG	5 cut(s)	956, 1117, 1246, 1489, 2653
PspGI	CCWGG	5 cut(s)	956, 1117, 1246, 1489, 2653
PspN4I	GGNNCC	6 cut(s)	128, 187, 1291, 1696, 2215, 2727
PspPI	GGNCC	5 cut(s)	1050, 1676, 2018, 2359, 2651
PstI	CTGCAG	1 cut(s)	880
PstNI	CAGNNNCTG	1 cut(s)	2638
PsuI	RGATCY	5 cut(s)	902, 952, 2047, 2272, 2350
RseI	CAYNNNNRTG	2 cut(s)	380, 680
SacI	GAGCTC	1 cut(s)	722
SatI	GCNGC	7 cut(s)	312, 645, 879, 1417, 1461, 1725, 2481
Sau96I	GGNCC	5 cut(s)	1050, 1676, 2018, 2359, 2651
SchI	GAGTC	4 cut(s)	326, 419, 841, 982
ScrFI	CCNGG	6 cut(s)	958, 1119, 1248, 1491, 1680, 2655
SduI	GDGCHC	8 cut(s)	722, 895, 1487, 1629, 1734, 1781, 2668, 2728
SexAI	ACCWGGT	1 cut(s)	2653
SfaNI	GCATC	5 cut(s)	676, 1614, 1927, 2414, 2506
SfcI	CTRYAG	2 cut(s)	876, 1548
SfoI	GGCGCC	1 cut(s)	128
SinI	GGWCC	4 cut(s)	1050, 1676, 2018, 2651
SmiMI	CAYNNNNRTG	2 cut(s)	380, 680
SmlI	CTYRAG	7 cut(s)	502, 1387, 1630, 1780, 2354, 2494, 2667
SmoI	CTYRAG	7 cut(s)	502, 1387, 1630, 1780, 2354, 2494, 2667
SnaBI	TACGTA	1 cut(s)	2503
SpeI	ACTAGT	1 cut(s)	91
SsiI	CCGC	5 cut(s)	41, 59, 663, 1417, 1724
SspDI	GGCGCC	1 cut(s)	126
SspI	AATATT	2 cut(s)	1099, 1576
SspMI	CTAG	3 cut(s)	92, 1317, 1950
SstI	GAGCTC	1 cut(s)	722
StyD4I	CCNGG	6 cut(s)	956, 1117, 1246, 1489, 1678, 2653
StyI	CCWWGG	1 cut(s)	1555
TaaI	ACNGT	6 cut(s)	947, 1582, 2092, 2539, 2553, 2622
TaiI	ACGT	3 cut(s)	1038, 2128, 2505
TaqI	TCGA	5 cut(s)	98, 368, 1646, 2136, 2541
TatI	WGTACW	4 cut(s)	256, 824, 942, 1928
TauI	GCSGC	2 cut(s)	1419, 1727
TscAI	CASTG	9 cut(s)	382, 952, 1068, 1264, 1668, 1734, 2182, 2233, 2698
TseFI	GTSAC	6 cut(s)	190, 373, 866, 1990, 2671, 2693
TseI	GCWGC	5 cut(s)	311, 644, 878, 1460, 2480
Tsp45I	GTSAC	6 cut(s)	190, 373, 866, 1990, 2671, 2693
TspGWI	ACGGA	6 cut(s)	60, 178, 630, 1532, 2559, 2718
TspRI	CASTG	9 cut(s)	382, 952, 1068, 1264, 1668, 1734, 2182, 2233, 2698
Van91I	CCANNNNNTGG	1 cut(s)	1477
VneI	GTGCAC	1 cut(s)	1625
VpaK11BI	GGWCC	4 cut(s)	1050, 1676, 2018, 2651
VspI	ATTAAT	1 cut(s)	1685
XagI	CCTNNNNNAGG	1 cut(s)	1179
XapI	RAATTY	5 cut(s)	263, 286, 1742, 1980, 2169
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1660
XmnI	GAANNNNTTC	1 cut(s)	1100
XspI	CTAG	3 cut(s)	92, 1317, 1950
Zsp2I	ATGCAT	1 cut(s)	1519

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.